AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1041220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1041220 MtrunA17_Chr7g0269451 100.000 114 0 0 1 114 75 188 1.63e-78 229
Msa1041220 MtrunA17_Chr1g0192271 90.351 114 10 1 1 114 75 187 1.26e-69 206
Msa1041220 MtrunA17_Chr7g0272381 86.842 114 14 1 1 114 75 187 5.94e-67 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1041220 AT5G27850.2 85.088 114 16 1 1 114 22 134 7.69e-67 197
Msa1041220 AT5G27850.1 85.088 114 16 1 1 114 75 187 2.01e-66 199
Msa1041220 AT3G05590.3 84.211 114 17 1 1 114 22 134 4.07e-66 196
Msa1041220 AT3G05590.2 84.211 114 17 1 1 114 22 134 4.07e-66 196
Msa1041220 AT3G05590.1 84.211 114 17 1 1 114 75 187 1.21e-65 196
Msa1041220 AT2G47570.1 74.783 115 27 2 1 114 73 186 7.19e-57 174

Find 50 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGAAGTACCTGCAATTA+AGG 0.289178 7_1:+78809505 Msa1041220:CDS
CTGGCTCTTAGAGCTCCTTT+AGG 0.297513 7_1:+78809605 Msa1041220:CDS
TGGCTCTTAGAGCTCCTTTA+GGG 0.321716 7_1:+78809606 Msa1041220:CDS
GTGCCGTGACAGATGACATT+AGG 0.331949 7_1:+78809480 Msa1041220:CDS
TGATAATGAGATATGATAAT+AGG 0.339000 7_1:-78810452 None:intergenic
AGGCTTTAAAGTTTGAGTTT+TGG 0.349919 7_1:+78810407 Msa1041220:exon
AGAGCAGTTACCTTAATTGC+AGG 0.362185 7_1:-78809515 None:intergenic
TTGAAGCCGCTAGAGGAAAA+AGG 0.383923 7_1:+78810376 Msa1041220:CDS
TGCCGTGACAGATGACATTA+GGG 0.403697 7_1:+78809481 Msa1041220:CDS
CAATTAAGGTAACTGCTCTA+AGG 0.406837 7_1:+78809519 Msa1041220:CDS
TTATCATATCTCATTATCAT+TGG 0.408608 7_1:+78810456 Msa1041220:three_prime_UTR
GTGCCTCACCTTTGATCAGC+TGG 0.415929 7_1:+78809586 Msa1041220:CDS
TCTTGTGCGCTCGTAGTGTT+GGG 0.441971 7_1:+78810659 Msa1041220:three_prime_UTR
TTCTTGTGCGCTCGTAGTGT+TGG 0.445083 7_1:+78810658 Msa1041220:three_prime_UTR
TCCTTCTCCCCAAGTGGAAA+AGG 0.454454 7_1:+78810539 Msa1041220:three_prime_UTR
TGCTCTAAGGTTCACCGAAA+AGG 0.469427 7_1:+78809532 Msa1041220:CDS
GGTAAGATTGCTGTAGTTGT+TGG 0.475236 7_1:+78809458 Msa1041220:CDS
AAGCACTTTGGACGAGCTCC+TGG 0.482073 7_1:+78810300 Msa1041220:CDS
CGTGAGGCTGTCAAGCACTT+TGG 0.482505 7_1:+78810288 Msa1041220:CDS
TAAGGTTCACCGAAAAGGCC+AGG 0.486544 7_1:+78809537 Msa1041220:CDS
GTGCGCTCGTAGTGTTGGGA+AGG 0.487077 7_1:+78810663 Msa1041220:three_prime_UTR
CATACCGTGTTCTGCCCTAA+AGG 0.491830 7_1:-78809620 None:intergenic
CGCTCGTAGTGTTGGGAAGG+GGG 0.492651 7_1:+78810666 Msa1041220:three_prime_UTR
ATGTTTCAGGTTCTTCTAAG+AGG 0.495334 7_1:+78810252 Msa1041220:intron
CCAAGTGGAAAAGGAATGAA+TGG 0.497670 7_1:+78810548 Msa1041220:three_prime_UTR
AGCCTTCTCAATTCTAGCCC+TGG 0.500353 7_1:-78809555 None:intergenic
ATTGTTCACAACACCACAAA+GGG 0.510119 7_1:-78810499 None:intergenic
TGAGATATGATAATAGGAAA+GGG 0.512617 7_1:-78810446 None:intergenic
GCGCTCGTAGTGTTGGGAAG+GGG 0.517946 7_1:+78810665 Msa1041220:three_prime_UTR
GTGTTGCTGTGTGGCACACC+AGG 0.524962 7_1:-78810318 None:intergenic
TGCGCTCGTAGTGTTGGGAA+GGG 0.527136 7_1:+78810664 Msa1041220:three_prime_UTR
TGGAAAAGGAATGAATGGAT+TGG 0.540570 7_1:+78810553 Msa1041220:three_prime_UTR
ATGAGATATGATAATAGGAA+AGG 0.543603 7_1:-78810447 None:intergenic
CTAGAATTGAGAAGGCTGGT+GGG 0.553032 7_1:+78809561 Msa1041220:CDS
AAAGTGCTTGACAGCCTCAC+GGG 0.558822 7_1:-78810286 None:intergenic
AGGGCTAGAATTGAGAAGGC+TGG 0.575107 7_1:+78809557 Msa1041220:CDS
CAAAGTGCTTGACAGCCTCA+CGG 0.580659 7_1:-78810287 None:intergenic
CGAAAGGTTGAAGCCGCTAG+AGG 0.583684 7_1:+78810369 Msa1041220:CDS
GCTAGAATTGAGAAGGCTGG+TGG 0.586828 7_1:+78809560 Msa1041220:CDS
TCTAAGAGCCAGCTGATCAA+AGG 0.589919 7_1:-78809594 None:intergenic
TAGAATTGAGAAGGCTGGTG+GGG 0.601753 7_1:+78809562 Msa1041220:CDS
TTAGCGTCCTTCTCCCCAAG+TGG 0.609019 7_1:+78810533 Msa1041220:three_prime_UTR
AACCCTAATGTCATCTGTCA+CGG 0.612086 7_1:-78809483 None:intergenic
GGCCAGGGCTAGAATTGAGA+AGG 0.617622 7_1:+78809553 Msa1041220:CDS
AGCTCCTTTAGGGCAGAACA+CGG 0.621376 7_1:+78809616 Msa1041220:CDS
AACACTAAGCCCTATGTCCG+TGG 0.624133 7_1:+78810336 Msa1041220:CDS
AAGGTTCACCGAAAAGGCCA+GGG 0.631182 7_1:+78809538 Msa1041220:CDS
AATTGTTCACAACACCACAA+AGG 0.654715 7_1:-78810500 None:intergenic
GAGCCAGCTGATCAAAGGTG+AGG 0.684975 7_1:-78809589 None:intergenic
TAGGGCTTAGTGTTGCTGTG+TGG 0.709531 7_1:-78810327 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTATAAACTTTTAATCTT+CGG - chr7_1:78809737-78809756 None:intergenic 15.0%
!!! TTTACTTTGTTTTGAAATAT+TGG + chr7_1:78809904-78809923 Msa1041220:intron 15.0%
!!! TGGTAAACATATTATTTTAT+AGG - chr7_1:78809992-78810011 None:intergenic 15.0%
!!! TGTTATAAACTTTTAATCTT+CGG - chr7_1:78809737-78809756 None:intergenic 15.0%
!!! TTTACTTTGTTTTGAAATAT+TGG + chr7_1:78809904-78809923 Msa1041220:intron 15.0%
!!! TGGTAAACATATTATTTTAT+AGG - chr7_1:78809992-78810011 None:intergenic 15.0%
!!! TATTATTTTATAGGCTGTAT+TGG - chr7_1:78809983-78810002 None:intergenic 20.0%
!! AACTAAAGCAAAATAAAAAC+AGG - chr7_1:78810031-78810050 None:intergenic 20.0%
!! TCCTAAAGTTGTAATAAAAA+AGG - chr7_1:78810145-78810164 None:intergenic 20.0%
!!! TTTTCTTGTATTAATGTTTC+AGG + chr7_1:78810239-78810258 Msa1041220:intron 20.0%
!!! TGATAATGAGATATGATAAT+AGG - chr7_1:78810455-78810474 None:intergenic 20.0%
!! TTATCATATCTCATTATCAT+TGG + chr7_1:78810456-78810475 Msa1041220:three_prime_UTR 20.0%
!!! TATTATTTTATAGGCTGTAT+TGG - chr7_1:78809983-78810002 None:intergenic 20.0%
!! AACTAAAGCAAAATAAAAAC+AGG - chr7_1:78810031-78810050 None:intergenic 20.0%
!! TCCTAAAGTTGTAATAAAAA+AGG - chr7_1:78810145-78810164 None:intergenic 20.0%
!!! TTTTCTTGTATTAATGTTTC+AGG + chr7_1:78810239-78810258 Msa1041220:intron 20.0%
!!! TGATAATGAGATATGATAAT+AGG - chr7_1:78810455-78810474 None:intergenic 20.0%
!! TTATCATATCTCATTATCAT+TGG + chr7_1:78810456-78810475 Msa1041220:three_prime_UTR 20.0%
!!! TTGCTGTAATTTTAGAAGTT+TGG + chr7_1:78809702-78809721 Msa1041220:intron 25.0%
! GAATAGAATGATAAATGAAG+AGG + chr7_1:78809865-78809884 Msa1041220:intron 25.0%
! ACAAAGTAAAACAAAAACAC+AGG - chr7_1:78809894-78809913 None:intergenic 25.0%
!! CAGAAAGTATAACAGTTATA+AGG - chr7_1:78809958-78809977 None:intergenic 25.0%
! ATAAAAAAGGCAGCAAAAAT+AGG - chr7_1:78810132-78810151 None:intergenic 25.0%
!!! GCCTTTTTTATTACAACTTT+AGG + chr7_1:78810141-78810160 Msa1041220:intron 25.0%
!! TTATTACAACTTTAGGAGAA+AGG + chr7_1:78810148-78810167 Msa1041220:intron 25.0%
! TGAGATATGATAATAGGAAA+GGG - chr7_1:78810449-78810468 None:intergenic 25.0%
! ATGAGATATGATAATAGGAA+AGG - chr7_1:78810450-78810469 None:intergenic 25.0%
!!! TTGCTGTAATTTTAGAAGTT+TGG + chr7_1:78809702-78809721 Msa1041220:intron 25.0%
! GAATAGAATGATAAATGAAG+AGG + chr7_1:78809865-78809884 Msa1041220:intron 25.0%
! ACAAAGTAAAACAAAAACAC+AGG - chr7_1:78809894-78809913 None:intergenic 25.0%
!! CAGAAAGTATAACAGTTATA+AGG - chr7_1:78809958-78809977 None:intergenic 25.0%
! ATAAAAAAGGCAGCAAAAAT+AGG - chr7_1:78810132-78810151 None:intergenic 25.0%
!!! GCCTTTTTTATTACAACTTT+AGG + chr7_1:78810141-78810160 Msa1041220:intron 25.0%
!! TTATTACAACTTTAGGAGAA+AGG + chr7_1:78810148-78810167 Msa1041220:intron 25.0%
! TGAGATATGATAATAGGAAA+GGG - chr7_1:78810449-78810468 None:intergenic 25.0%
! ATGAGATATGATAATAGGAA+AGG - chr7_1:78810450-78810469 None:intergenic 25.0%
! TTATGAAGTACCTGCAATTA+AGG + chr7_1:78809505-78809524 Msa1041220:CDS 30.0%
CAATTGCATGAAGTTTAGTT+TGG - chr7_1:78809770-78809789 None:intergenic 30.0%
AAAACATGACTTCATACAAG+TGG - chr7_1:78809807-78809826 None:intergenic 30.0%
!! TTGTCTTAATTTGCAAAGAG+TGG - chr7_1:78810197-78810216 None:intergenic 30.0%
ATTAAGACAACATACATCTG+AGG + chr7_1:78810207-78810226 Msa1041220:intron 30.0%
!!! AGGCTTTAAAGTTTGAGTTT+TGG + chr7_1:78810407-78810426 Msa1041220:exon 30.0%
! TTATGAAGTACCTGCAATTA+AGG + chr7_1:78809505-78809524 Msa1041220:CDS 30.0%
CAATTGCATGAAGTTTAGTT+TGG - chr7_1:78809770-78809789 None:intergenic 30.0%
AAAACATGACTTCATACAAG+TGG - chr7_1:78809807-78809826 None:intergenic 30.0%
!! TTGTCTTAATTTGCAAAGAG+TGG - chr7_1:78810197-78810216 None:intergenic 30.0%
ATTAAGACAACATACATCTG+AGG + chr7_1:78810207-78810226 Msa1041220:intron 30.0%
!!! AGGCTTTAAAGTTTGAGTTT+TGG + chr7_1:78810407-78810426 Msa1041220:exon 30.0%
CAATTAAGGTAACTGCTCTA+AGG + chr7_1:78809519-78809538 Msa1041220:CDS 35.0%
! TTGGATAATGCTGTTACTTG+CGG + chr7_1:78809923-78809942 Msa1041220:intron 35.0%
! GCGGCAAATTAGATCAATTT+TGG + chr7_1:78810087-78810106 Msa1041220:intron 35.0%
!! ATGTTTCAGGTTCTTCTAAG+AGG + chr7_1:78810252-78810271 Msa1041220:intron 35.0%
!!! GATTTTTTTCCACGGACATA+GGG - chr7_1:78810348-78810367 None:intergenic 35.0%
!!! TGATTTTTTTCCACGGACAT+AGG - chr7_1:78810349-78810368 None:intergenic 35.0%
!!! CCTTTCGTGATTTTTTTCCA+CGG - chr7_1:78810356-78810375 None:intergenic 35.0%
!!! TGTTTTGTTTTCTCCCTTTG+TGG + chr7_1:78810486-78810505 Msa1041220:three_prime_UTR 35.0%
ATTGTTCACAACACCACAAA+GGG - chr7_1:78810502-78810521 None:intergenic 35.0%
AATTGTTCACAACACCACAA+AGG - chr7_1:78810503-78810522 None:intergenic 35.0%
! ATTCATTCCTTTTCCACTTG+GGG - chr7_1:78810549-78810568 None:intergenic 35.0%
! CATTCATTCCTTTTCCACTT+GGG - chr7_1:78810550-78810569 None:intergenic 35.0%
TGGAAAAGGAATGAATGGAT+TGG + chr7_1:78810553-78810572 Msa1041220:three_prime_UTR 35.0%
CAATTAAGGTAACTGCTCTA+AGG + chr7_1:78809519-78809538 Msa1041220:CDS 35.0%
! TTGGATAATGCTGTTACTTG+CGG + chr7_1:78809923-78809942 Msa1041220:intron 35.0%
! GCGGCAAATTAGATCAATTT+TGG + chr7_1:78810087-78810106 Msa1041220:intron 35.0%
!! ATGTTTCAGGTTCTTCTAAG+AGG + chr7_1:78810252-78810271 Msa1041220:intron 35.0%
!!! GATTTTTTTCCACGGACATA+GGG - chr7_1:78810348-78810367 None:intergenic 35.0%
!!! TGATTTTTTTCCACGGACAT+AGG - chr7_1:78810349-78810368 None:intergenic 35.0%
!!! CCTTTCGTGATTTTTTTCCA+CGG - chr7_1:78810356-78810375 None:intergenic 35.0%
!!! TGTTTTGTTTTCTCCCTTTG+TGG + chr7_1:78810486-78810505 Msa1041220:three_prime_UTR 35.0%
ATTGTTCACAACACCACAAA+GGG - chr7_1:78810502-78810521 None:intergenic 35.0%
AATTGTTCACAACACCACAA+AGG - chr7_1:78810503-78810522 None:intergenic 35.0%
! ATTCATTCCTTTTCCACTTG+GGG - chr7_1:78810549-78810568 None:intergenic 35.0%
! CATTCATTCCTTTTCCACTT+GGG - chr7_1:78810550-78810569 None:intergenic 35.0%
TGGAAAAGGAATGAATGGAT+TGG + chr7_1:78810553-78810572 Msa1041220:three_prime_UTR 35.0%
GGTAAGATTGCTGTAGTTGT+TGG + chr7_1:78809458-78809477 Msa1041220:CDS 40.0%
AACCCTAATGTCATCTGTCA+CGG - chr7_1:78809486-78809505 None:intergenic 40.0%
AGAGCAGTTACCTTAATTGC+AGG - chr7_1:78809518-78809537 None:intergenic 40.0%
! CAGGAAGTACACTAGATTTG+TGG - chr7_1:78810012-78810031 None:intergenic 40.0%
ACTTCTATACCTATCTGCTG+CGG + chr7_1:78810068-78810087 Msa1041220:intron 40.0%
CCGTGGAAAAAAATCACGAA+AGG + chr7_1:78810353-78810372 Msa1041220:CDS 40.0%
! CCATTCATTCCTTTTCCACT+TGG - chr7_1:78810551-78810570 None:intergenic 40.0%
CCAAGTGGAAAAGGAATGAA+TGG + chr7_1:78810548-78810567 Msa1041220:three_prime_UTR 40.0%
GGTAAGATTGCTGTAGTTGT+TGG + chr7_1:78809458-78809477 Msa1041220:CDS 40.0%
AACCCTAATGTCATCTGTCA+CGG - chr7_1:78809486-78809505 None:intergenic 40.0%
AGAGCAGTTACCTTAATTGC+AGG - chr7_1:78809518-78809537 None:intergenic 40.0%
! CAGGAAGTACACTAGATTTG+TGG - chr7_1:78810012-78810031 None:intergenic 40.0%
ACTTCTATACCTATCTGCTG+CGG + chr7_1:78810068-78810087 Msa1041220:intron 40.0%
CCGTGGAAAAAAATCACGAA+AGG + chr7_1:78810353-78810372 Msa1041220:CDS 40.0%
! CCATTCATTCCTTTTCCACT+TGG - chr7_1:78810551-78810570 None:intergenic 40.0%
CCAAGTGGAAAAGGAATGAA+TGG + chr7_1:78810548-78810567 Msa1041220:three_prime_UTR 40.0%
TGCCGTGACAGATGACATTA+GGG + chr7_1:78809481-78809500 Msa1041220:CDS 45.0%
!! TGCTCTAAGGTTCACCGAAA+AGG + chr7_1:78809532-78809551 Msa1041220:CDS 45.0%
!! AATTCTAGCCCTGGCCTTTT+CGG - chr7_1:78809549-78809568 None:intergenic 45.0%
CTAGAATTGAGAAGGCTGGT+GGG + chr7_1:78809561-78809580 Msa1041220:CDS 45.0%
TAGAATTGAGAAGGCTGGTG+GGG + chr7_1:78809562-78809581 Msa1041220:CDS 45.0%
TCTAAGAGCCAGCTGATCAA+AGG - chr7_1:78809597-78809616 None:intergenic 45.0%
TGGCTCTTAGAGCTCCTTTA+GGG + chr7_1:78809606-78809625 Msa1041220:CDS 45.0%
! TCTAATTTGCCGCAGCAGAT+AGG - chr7_1:78810080-78810099 None:intergenic 45.0%
TTGAAGCCGCTAGAGGAAAA+AGG + chr7_1:78810376-78810395 Msa1041220:CDS 45.0%
! GCTCTTCCTTTTTCCTCTAG+CGG - chr7_1:78810385-78810404 None:intergenic 45.0%
AGAGGAAAAAGGAAGAGCAG+AGG + chr7_1:78810387-78810406 Msa1041220:CDS 45.0%
TGCCGTGACAGATGACATTA+GGG + chr7_1:78809481-78809500 Msa1041220:CDS 45.0%
!! TGCTCTAAGGTTCACCGAAA+AGG + chr7_1:78809532-78809551 Msa1041220:CDS 45.0%
!! AATTCTAGCCCTGGCCTTTT+CGG - chr7_1:78809549-78809568 None:intergenic 45.0%
CTAGAATTGAGAAGGCTGGT+GGG + chr7_1:78809561-78809580 Msa1041220:CDS 45.0%
TAGAATTGAGAAGGCTGGTG+GGG + chr7_1:78809562-78809581 Msa1041220:CDS 45.0%
TCTAAGAGCCAGCTGATCAA+AGG - chr7_1:78809597-78809616 None:intergenic 45.0%
TGGCTCTTAGAGCTCCTTTA+GGG + chr7_1:78809606-78809625 Msa1041220:CDS 45.0%
! TCTAATTTGCCGCAGCAGAT+AGG - chr7_1:78810080-78810099 None:intergenic 45.0%
TTGAAGCCGCTAGAGGAAAA+AGG + chr7_1:78810376-78810395 Msa1041220:CDS 45.0%
! GCTCTTCCTTTTTCCTCTAG+CGG - chr7_1:78810385-78810404 None:intergenic 45.0%
AGAGGAAAAAGGAAGAGCAG+AGG + chr7_1:78810387-78810406 Msa1041220:CDS 45.0%
GTGCCGTGACAGATGACATT+AGG + chr7_1:78809480-78809499 Msa1041220:CDS 50.0%
TAAGGTTCACCGAAAAGGCC+AGG + chr7_1:78809537-78809556 Msa1041220:CDS 50.0%
AAGGTTCACCGAAAAGGCCA+GGG + chr7_1:78809538-78809557 Msa1041220:CDS 50.0%
AGCCTTCTCAATTCTAGCCC+TGG - chr7_1:78809558-78809577 None:intergenic 50.0%
AGGGCTAGAATTGAGAAGGC+TGG + chr7_1:78809557-78809576 Msa1041220:CDS 50.0%
GCTAGAATTGAGAAGGCTGG+TGG + chr7_1:78809560-78809579 Msa1041220:CDS 50.0%
! CTGGCTCTTAGAGCTCCTTT+AGG + chr7_1:78809605-78809624 Msa1041220:CDS 50.0%
!! AGCTCCTTTAGGGCAGAACA+CGG + chr7_1:78809616-78809635 Msa1041220:CDS 50.0%
CATACCGTGTTCTGCCCTAA+AGG - chr7_1:78809623-78809642 None:intergenic 50.0%
! ACAGCCTCACGGGCATTTTT+GGG - chr7_1:78810279-78810298 None:intergenic 50.0%
! AAAGTGCTTGACAGCCTCAC+GGG - chr7_1:78810289-78810308 None:intergenic 50.0%
! CAAAGTGCTTGACAGCCTCA+CGG - chr7_1:78810290-78810309 None:intergenic 50.0%
! TAGGGCTTAGTGTTGCTGTG+TGG - chr7_1:78810330-78810349 None:intergenic 50.0%
AACACTAAGCCCTATGTCCG+TGG + chr7_1:78810336-78810355 Msa1041220:CDS 50.0%
TCCTTCTCCCCAAGTGGAAA+AGG + chr7_1:78810539-78810558 Msa1041220:three_prime_UTR 50.0%
! TCCTTTTCCACTTGGGGAGA+AGG - chr7_1:78810543-78810562 None:intergenic 50.0%
TTCTTGTGCGCTCGTAGTGT+TGG + chr7_1:78810658-78810677 Msa1041220:three_prime_UTR 50.0%
TCTTGTGCGCTCGTAGTGTT+GGG + chr7_1:78810659-78810678 Msa1041220:three_prime_UTR 50.0%
GTGCCGTGACAGATGACATT+AGG + chr7_1:78809480-78809499 Msa1041220:CDS 50.0%
TAAGGTTCACCGAAAAGGCC+AGG + chr7_1:78809537-78809556 Msa1041220:CDS 50.0%
AAGGTTCACCGAAAAGGCCA+GGG + chr7_1:78809538-78809557 Msa1041220:CDS 50.0%
AGCCTTCTCAATTCTAGCCC+TGG - chr7_1:78809558-78809577 None:intergenic 50.0%
AGGGCTAGAATTGAGAAGGC+TGG + chr7_1:78809557-78809576 Msa1041220:CDS 50.0%
GCTAGAATTGAGAAGGCTGG+TGG + chr7_1:78809560-78809579 Msa1041220:CDS 50.0%
! CTGGCTCTTAGAGCTCCTTT+AGG + chr7_1:78809605-78809624 Msa1041220:CDS 50.0%
!! AGCTCCTTTAGGGCAGAACA+CGG + chr7_1:78809616-78809635 Msa1041220:CDS 50.0%
CATACCGTGTTCTGCCCTAA+AGG - chr7_1:78809623-78809642 None:intergenic 50.0%
! ACAGCCTCACGGGCATTTTT+GGG - chr7_1:78810279-78810298 None:intergenic 50.0%
! AAAGTGCTTGACAGCCTCAC+GGG - chr7_1:78810289-78810308 None:intergenic 50.0%
! CAAAGTGCTTGACAGCCTCA+CGG - chr7_1:78810290-78810309 None:intergenic 50.0%
! TAGGGCTTAGTGTTGCTGTG+TGG - chr7_1:78810330-78810349 None:intergenic 50.0%
AACACTAAGCCCTATGTCCG+TGG + chr7_1:78810336-78810355 Msa1041220:CDS 50.0%
TCCTTCTCCCCAAGTGGAAA+AGG + chr7_1:78810539-78810558 Msa1041220:three_prime_UTR 50.0%
! TCCTTTTCCACTTGGGGAGA+AGG - chr7_1:78810543-78810562 None:intergenic 50.0%
TTCTTGTGCGCTCGTAGTGT+TGG + chr7_1:78810658-78810677 Msa1041220:three_prime_UTR 50.0%
TCTTGTGCGCTCGTAGTGTT+GGG + chr7_1:78810659-78810678 Msa1041220:three_prime_UTR 50.0%
GGCCAGGGCTAGAATTGAGA+AGG + chr7_1:78809553-78809572 Msa1041220:CDS 55.0%
GTGCCTCACCTTTGATCAGC+TGG + chr7_1:78809586-78809605 Msa1041220:CDS 55.0%
GAGCCAGCTGATCAAAGGTG+AGG - chr7_1:78809592-78809611 None:intergenic 55.0%
AGGTCCCAAAAATGCCCGTG+AGG + chr7_1:78810272-78810291 Msa1041220:CDS 55.0%
! GACAGCCTCACGGGCATTTT+TGG - chr7_1:78810280-78810299 None:intergenic 55.0%
CGTGAGGCTGTCAAGCACTT+TGG + chr7_1:78810288-78810307 Msa1041220:CDS 55.0%
AAGCACTTTGGACGAGCTCC+TGG + chr7_1:78810300-78810319 Msa1041220:CDS 55.0%
CGAAAGGTTGAAGCCGCTAG+AGG + chr7_1:78810369-78810388 Msa1041220:CDS 55.0%
TTAGCGTCCTTCTCCCCAAG+TGG + chr7_1:78810533-78810552 Msa1041220:three_prime_UTR 55.0%
! TGCGCTCGTAGTGTTGGGAA+GGG + chr7_1:78810664-78810683 Msa1041220:three_prime_UTR 55.0%
GGCCAGGGCTAGAATTGAGA+AGG + chr7_1:78809553-78809572 Msa1041220:CDS 55.0%
GTGCCTCACCTTTGATCAGC+TGG + chr7_1:78809586-78809605 Msa1041220:CDS 55.0%
GAGCCAGCTGATCAAAGGTG+AGG - chr7_1:78809592-78809611 None:intergenic 55.0%
AGGTCCCAAAAATGCCCGTG+AGG + chr7_1:78810272-78810291 Msa1041220:CDS 55.0%
! GACAGCCTCACGGGCATTTT+TGG - chr7_1:78810280-78810299 None:intergenic 55.0%
CGTGAGGCTGTCAAGCACTT+TGG + chr7_1:78810288-78810307 Msa1041220:CDS 55.0%
AAGCACTTTGGACGAGCTCC+TGG + chr7_1:78810300-78810319 Msa1041220:CDS 55.0%
CGAAAGGTTGAAGCCGCTAG+AGG + chr7_1:78810369-78810388 Msa1041220:CDS 55.0%
TTAGCGTCCTTCTCCCCAAG+TGG + chr7_1:78810533-78810552 Msa1041220:three_prime_UTR 55.0%
! TGCGCTCGTAGTGTTGGGAA+GGG + chr7_1:78810664-78810683 Msa1041220:three_prime_UTR 55.0%
! GTGTTGCTGTGTGGCACACC+AGG - chr7_1:78810321-78810340 None:intergenic 60.0%
! GTGCGCTCGTAGTGTTGGGA+AGG + chr7_1:78810663-78810682 Msa1041220:three_prime_UTR 60.0%
! GCGCTCGTAGTGTTGGGAAG+GGG + chr7_1:78810665-78810684 Msa1041220:three_prime_UTR 60.0%
! CGCTCGTAGTGTTGGGAAGG+GGG + chr7_1:78810666-78810685 Msa1041220:three_prime_UTR 60.0%
! GTGTTGCTGTGTGGCACACC+AGG - chr7_1:78810321-78810340 None:intergenic 60.0%
! GTGCGCTCGTAGTGTTGGGA+AGG + chr7_1:78810663-78810682 Msa1041220:three_prime_UTR 60.0%
! GCGCTCGTAGTGTTGGGAAG+GGG + chr7_1:78810665-78810684 Msa1041220:three_prime_UTR 60.0%
! CGCTCGTAGTGTTGGGAAGG+GGG + chr7_1:78810666-78810685 Msa1041220:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 78809455 78810695 78809455 ID=Msa1041220;Name=Msa1041220
chr7_1 mRNA 78809455 78810695 78809455 ID=Msa1041220-mRNA-1;Parent=Msa1041220;Name=Msa1041220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|273|114
chr7_1 exon 78809455 78809637 78809455 ID=Msa1041220-mRNA-1:exon:21084;Parent=Msa1041220-mRNA-1
chr7_1 exon 78810261 78810695 78810261 ID=Msa1041220-mRNA-1:exon:21085;Parent=Msa1041220-mRNA-1
chr7_1 CDS 78809455 78809637 78809455 ID=Msa1041220-mRNA-1:cds;Parent=Msa1041220-mRNA-1
chr7_1 CDS 78810261 78810422 78810261 ID=Msa1041220-mRNA-1:cds;Parent=Msa1041220-mRNA-1
chr7_1 three_prime_UTR 78810423 78810695 78810423 ID=Msa1041220-mRNA-1:three_prime_utr;Parent=Msa1041220-mRNA-1
Gene Sequence

>Msa1041220

GAGGGTAAGATTGCTGTAGTTGTTGGTGCCGTGACAGATGACATTAGGGTTTATGAAGTACCTGCAATTAAGGTAACTGCTCTAAGGTTCACCGAAAAGGCCAGGGCTAGAATTGAGAAGGCTGGTGGGGAGTGCCTCACCTTTGATCAGCTGGCTCTTAGAGCTCCTTTAGGGCAGAACACGGTTCTTCTAAGAGGTCCCAAAAATGCCCGTGAGGCTGTCAAGCACTTTGGACGAGCTCCTGGTGTGCCACACAGCAACACTAAGCCCTATGTCCGTGGAAAAAAATCACGAAAGGTTGAAGCCGCTAGAGGAAAAAGGAAGAGCAGAGGCTTTAAAGTTTGA
Protein sequence

>Msa1041220

EGKIAVVVGAVTDDIRVYEVPAIKVTALRFTEKARARIEKAGGECLTFDQLALRAPLGQNTVLLRGPKNAREAVKHFGRAPGVPHSNTKPYVRGKKSRKVEAARGKRKSRGFKV*