Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1043410 | Msa1043470 | 0.825180 | 5.393509e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGGGAAATGGCATAAT+AGG | 0.306645 | 7_2:-1649741 | None:intergenic |
| GAGTAGCTCAGCATAATTTC+AGG | 0.376396 | 7_2:-1649434 | None:intergenic |
| ATTGTTAGTTCTTTACATTA+AGG | 0.392365 | 7_2:+1649701 | Msa1043470:three_prime_UTR |
| ATGTTATTATAGAGAGGGGT+TGG | 0.476331 | 7_2:+1649404 | Msa1043470:five_prime_UTR |
| TCTGAGGTGCTCCCTGCTGC+AGG | 0.481916 | 7_2:+1649621 | Msa1043470:CDS |
| TGTGTATGTTATTATAGAGA+GGG | 0.534235 | 7_2:+1649399 | Msa1043470:five_prime_UTR |
| ATGTGTATGTTATTATAGAG+AGG | 0.569595 | 7_2:+1649398 | Msa1043470:five_prime_UTR |
| GTGTATGTTATTATAGAGAG+GGG | 0.605967 | 7_2:+1649400 | Msa1043470:five_prime_UTR |
| AAAGAACTAACAATAAAGAG+AGG | 0.665842 | 7_2:-1649692 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAAAAATACTTTCAATTAG+TGG | + | chr7_2:1649527-1649546 | Msa1043470:intron | 20.0% |
| !!! | ATTGTTAGTTCTTTACATTA+AGG | + | chr7_2:1649701-1649720 | Msa1043470:three_prime_UTR | 20.0% |
| ! | ATGTGTATGTTATTATAGAG+AGG | + | chr7_2:1649398-1649417 | Msa1043470:five_prime_UTR | 25.0% |
| ! | TGTGTATGTTATTATAGAGA+GGG | + | chr7_2:1649399-1649418 | Msa1043470:five_prime_UTR | 25.0% |
| ! | AAAGAACTAACAATAAAGAG+AGG | - | chr7_2:1649695-1649714 | None:intergenic | 25.0% |
| GTGTATGTTATTATAGAGAG+GGG | + | chr7_2:1649400-1649419 | Msa1043470:five_prime_UTR | 30.0% | |
| ! | TTAGTGGCTATTTCATTGTT+AGG | + | chr7_2:1649543-1649562 | Msa1043470:intron | 30.0% |
| !! | AAATTAGACAAAGGCATTCT+AGG | - | chr7_2:1649576-1649595 | None:intergenic | 30.0% |
| !! | TAATTTTCGTCGTTGTTTTC+AGG | + | chr7_2:1649590-1649609 | Msa1043470:intron | 30.0% |
| ATGTTATTATAGAGAGGGGT+TGG | + | chr7_2:1649404-1649423 | Msa1043470:five_prime_UTR | 35.0% | |
| CAACGACGAAAATTAGACAA+AGG | - | chr7_2:1649585-1649604 | None:intergenic | 35.0% | |
| GAGTAGCTCAGCATAATTTC+AGG | - | chr7_2:1649437-1649456 | None:intergenic | 40.0% | |
| ! | GTGGCTATTTCATTGTTAGG+TGG | + | chr7_2:1649546-1649565 | Msa1043470:intron | 40.0% |
| !! | TACCATTTTTACCTGCAGCA+GGG | - | chr7_2:1649635-1649654 | None:intergenic | 40.0% |
| !! | ATACCATTTTTACCTGCAGC+AGG | - | chr7_2:1649636-1649655 | None:intergenic | 40.0% |
| !! | TTTTCAGGCTGCGAATTCTG+AGG | + | chr7_2:1649605-1649624 | Msa1043470:intron | 45.0% |
| CTCCCTGCTGCAGGTAAAAA+TGG | + | chr7_2:1649630-1649649 | Msa1043470:CDS | 50.0% | |
| ! | TCTGAGGTGCTCCCTGCTGC+AGG | + | chr7_2:1649621-1649640 | Msa1043470:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 1649391 | 1649751 | 1649391 | ID=Msa1043470;Name=Msa1043470 |
| chr7_2 | mRNA | 1649391 | 1649751 | 1649391 | ID=Msa1043470-mRNA-1;Parent=Msa1043470;Name=Msa1043470-mRNA-1;_AED=0.00;_eAED=0.00;_QI=52|1|1|1|1|1|2|85|44 |
| chr7_2 | exon | 1649391 | 1649522 | 1649391 | ID=Msa1043470-mRNA-1:exon:265;Parent=Msa1043470-mRNA-1 |
| chr7_2 | exon | 1649612 | 1649751 | 1649612 | ID=Msa1043470-mRNA-1:exon:266;Parent=Msa1043470-mRNA-1 |
| chr7_2 | five_prime_UTR | 1649391 | 1649442 | 1649391 | ID=Msa1043470-mRNA-1:five_prime_utr;Parent=Msa1043470-mRNA-1 |
| chr7_2 | CDS | 1649443 | 1649522 | 1649443 | ID=Msa1043470-mRNA-1:cds;Parent=Msa1043470-mRNA-1 |
| chr7_2 | CDS | 1649612 | 1649666 | 1649612 | ID=Msa1043470-mRNA-1:cds;Parent=Msa1043470-mRNA-1 |
| chr7_2 | three_prime_UTR | 1649667 | 1649751 | 1649667 | ID=Msa1043470-mRNA-1:three_prime_utr;Parent=Msa1043470-mRNA-1 |
| Gene Sequence |
| Protein sequence |
