Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0766630 | Msa1043410 | 0.813611 | 2.345296e-51 | -8.615850e-47 |
| Msa0131770 | Msa1043410 | 0.804349 | 2.254408e-49 | -8.615850e-47 |
| Msa1014540 | Msa1043410 | 0.800305 | 1.534146e-48 | -8.615850e-47 |
| Msa1043410 | Msa1043470 | 0.825180 | 5.393509e-54 | -8.615850e-47 |
| Msa1043410 | Msa1075220 | -0.801830 | 7.482582e-49 | -8.615850e-47 |
| Msa1043410 | Msa1284680 | 0.812834 | 3.472397e-51 | -8.615850e-47 |
| Msa0270520 | Msa1043410 | 0.806578 | 7.685253e-50 | -8.615850e-47 |
| Msa0885280 | Msa1043410 | 0.801489 | 8.790312e-49 | -8.615850e-47 |
| Msa0885290 | Msa1043410 | 0.810730 | 9.966810e-51 | -8.615850e-47 |
| Msa0954910 | Msa1043410 | 0.802364 | 5.811146e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGGGAAATGGCATAAT+AGG | 0.306645 | 7_2:-1613060 | None:intergenic |
| GAGTAGCTCAGCATAATTTC+AGG | 0.376396 | 7_2:-1612753 | None:intergenic |
| ATTGTTAGTTCTTTACATTA+AGG | 0.392365 | 7_2:+1613020 | Msa1043410:three_prime_UTR |
| ATGTTATTATAGAGAGGGGT+TGG | 0.476331 | 7_2:+1612723 | Msa1043410:five_prime_UTR |
| TCTGAGGTGCTCCCTGCTGC+AGG | 0.481916 | 7_2:+1612940 | Msa1043410:CDS |
| TGTGTATGTTATTATAGAGA+GGG | 0.534235 | 7_2:+1612718 | Msa1043410:five_prime_UTR |
| ATGTGTATGTTATTATAGAG+AGG | 0.569595 | 7_2:+1612717 | Msa1043410:five_prime_UTR |
| GTGTATGTTATTATAGAGAG+GGG | 0.605967 | 7_2:+1612719 | Msa1043410:five_prime_UTR |
| AAAGAACTAACAATAAAGAG+AGG | 0.665842 | 7_2:-1613011 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAAAAATACTTTCAATTAG+TGG | + | chr7_2:1612846-1612865 | Msa1043410:intron | 20.0% |
| !!! | ATTGTTAGTTCTTTACATTA+AGG | + | chr7_2:1613020-1613039 | Msa1043410:three_prime_UTR | 20.0% |
| ! | ATGTGTATGTTATTATAGAG+AGG | + | chr7_2:1612717-1612736 | Msa1043410:five_prime_UTR | 25.0% |
| ! | TGTGTATGTTATTATAGAGA+GGG | + | chr7_2:1612718-1612737 | Msa1043410:five_prime_UTR | 25.0% |
| ! | AAAGAACTAACAATAAAGAG+AGG | - | chr7_2:1613014-1613033 | None:intergenic | 25.0% |
| GTGTATGTTATTATAGAGAG+GGG | + | chr7_2:1612719-1612738 | Msa1043410:five_prime_UTR | 30.0% | |
| ! | TTAGTGGCTATTTCATTGTT+AGG | + | chr7_2:1612862-1612881 | Msa1043410:intron | 30.0% |
| !! | AAATTAGACAAAGGCATTCT+AGG | - | chr7_2:1612895-1612914 | None:intergenic | 30.0% |
| !! | TAATTTTCGTCGTTGTTTTC+AGG | + | chr7_2:1612909-1612928 | Msa1043410:intron | 30.0% |
| ATGTTATTATAGAGAGGGGT+TGG | + | chr7_2:1612723-1612742 | Msa1043410:five_prime_UTR | 35.0% | |
| CAACGACGAAAATTAGACAA+AGG | - | chr7_2:1612904-1612923 | None:intergenic | 35.0% | |
| GAGTAGCTCAGCATAATTTC+AGG | - | chr7_2:1612756-1612775 | None:intergenic | 40.0% | |
| ! | GTGGCTATTTCATTGTTAGG+TGG | + | chr7_2:1612865-1612884 | Msa1043410:intron | 40.0% |
| !! | TACCATTTTTACCTGCAGCA+GGG | - | chr7_2:1612954-1612973 | None:intergenic | 40.0% |
| !! | ATACCATTTTTACCTGCAGC+AGG | - | chr7_2:1612955-1612974 | None:intergenic | 40.0% |
| !! | TTTTCAGGCTGCGAATTCTG+AGG | + | chr7_2:1612924-1612943 | Msa1043410:intron | 45.0% |
| CTCCCTGCTGCAGGTAAAAA+TGG | + | chr7_2:1612949-1612968 | Msa1043410:CDS | 50.0% | |
| ! | TCTGAGGTGCTCCCTGCTGC+AGG | + | chr7_2:1612940-1612959 | Msa1043410:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 1612710 | 1613070 | 1612710 | ID=Msa1043410;Name=Msa1043410 |
| chr7_2 | mRNA | 1612710 | 1613070 | 1612710 | ID=Msa1043410-mRNA-1;Parent=Msa1043410;Name=Msa1043410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=52|1|1|1|1|1|2|85|44 |
| chr7_2 | exon | 1612710 | 1612841 | 1612710 | ID=Msa1043410-mRNA-1:exon:263;Parent=Msa1043410-mRNA-1 |
| chr7_2 | exon | 1612931 | 1613070 | 1612931 | ID=Msa1043410-mRNA-1:exon:264;Parent=Msa1043410-mRNA-1 |
| chr7_2 | five_prime_UTR | 1612710 | 1612761 | 1612710 | ID=Msa1043410-mRNA-1:five_prime_utr;Parent=Msa1043410-mRNA-1 |
| chr7_2 | CDS | 1612762 | 1612841 | 1612762 | ID=Msa1043410-mRNA-1:cds;Parent=Msa1043410-mRNA-1 |
| chr7_2 | CDS | 1612931 | 1612985 | 1612931 | ID=Msa1043410-mRNA-1:cds;Parent=Msa1043410-mRNA-1 |
| chr7_2 | three_prime_UTR | 1612986 | 1613070 | 1612986 | ID=Msa1043410-mRNA-1:three_prime_utr;Parent=Msa1043410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
