AlfalfaGEDB | Msa1048550

M. sativa cultivar zhongmu-4 / Msa1048550

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1048550	AES77386.1	78.755	273	28	1	6	248	595	867	3.34e-141	427

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1048550	sp\|Q8VZG8\|MIK2_ARATH	42.241	232	102	4	16	216	814	1044	3.93e-53	187

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1048550	G7KZX4	78.755	273	28	1	6	248	595	867	1.60e-141	427

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0533430	Msa1048550	0.810552	1.089196e-50	-8.615850e-47
Msa0580610	Msa1048550	0.802207	6.260093e-49	-8.615850e-47
Msa0784660	Msa1048550	0.802661	5.046621e-49	-8.615850e-47
Msa0818010	Msa1048550	0.816786	4.622245e-52	-8.615850e-47
Msa0884920	Msa1048550	0.835387	1.728082e-56	-8.615850e-47
Msa1006360	Msa1048550	0.819323	1.233807e-52	-8.615850e-47
Msa1048490	Msa1048550	0.811754	5.977474e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1048550	MtrunA17_Chr7g0217081	78.755	273	28	1	6	248	595	867	3.08e-145	427
Msa1048550	MtrunA17_Chr7g0217061	74.632	272	39	1	8	249	520	791	3.10e-135	399
Msa1048550	MtrunA17_Chr7g0215651	69.742	271	52	1	8	248	470	740	1.67e-125	373
Msa1048550	MtrunA17_Chr7g0215681	69.373	271	53	1	8	248	517	787	3.64e-124	370
Msa1048550	MtrunA17_Chr7g0215631	69.004	271	54	1	8	248	479	749	6.38e-123	366
Msa1048550	MtrunA17_Chr7g0215061	68.266	271	56	1	8	248	558	828	5.71e-122	366
Msa1048550	MtrunA17_Chr7g0215641	67.159	271	59	1	8	248	504	774	2.50e-121	363
Msa1048550	MtrunA17_Chr7g0218991	67.159	271	59	2	8	248	493	763	6.39e-118	354
Msa1048550	MtrunA17_Chr7g0215011	69.888	269	51	1	8	246	517	785	9.64e-117	351
Msa1048550	MtrunA17_Chr7g0215671	68.635	271	55	1	8	248	486	756	1.17e-116	350

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1048550	AT4G08850.1	42.241	232	102	4	16	216	814	1044	3.99e-54	187
Msa1048550	AT1G35710.1	41.597	238	104	4	11	216	885	1119	7.67e-54	187
Msa1048550	AT1G17230.1	33.607	244	123	3	7	212	826	1068	2.67e-37	139
Msa1048550	AT1G17230.2	33.607	244	123	3	7	212	826	1068	2.69e-37	139
Msa1048550	AT5G63930.1	32.911	237	118	4	14	212	841	1074	3.22e-35	133
Msa1048550	AT5G63930.2	32.911	237	118	4	14	212	777	1010	3.24e-35	133
Msa1048550	AT4G08850.2	53.333	105	49	0	16	120	814	918	8.91e-34	129
Msa1048550	AT3G49670.1	35.361	263	122	10	7	229	717	971	2.36e-32	125
Msa1048550	AT5G65700.1	32.971	276	133	9	7	237	721	989	3.92e-32	124
Msa1048550	AT5G65700.2	32.971	276	133	9	7	237	721	989	3.92e-32	124

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Find 33 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTTAATGTTGTGAAAG+AGG	0.419016	7_2:+10316202	Msa1048550:CDS
AAGTGTGATGTATATAGTTT+TGG	0.407970	7_2:+10316895	Msa1048550:CDS
ACATCTCTATGCACTATTGG+AGG	0.532094	7_2:-10316263	None:intergenic
ACCTTCACATTATTTGGAAG+TGG	0.481399	7_2:-10317030	None:intergenic
ACGGAGGGAGTATGAAGCAG+AGG	0.640255	7_2:+10315986	Msa1048550:CDS
ACTTTAATGGGAAGACACCC+TGG	0.558199	7_2:+10316934	Msa1048550:CDS
AGTAAAACCTTCACATTATT+TGG	0.120792	7_2:-10317036	None:intergenic
AGTGATGACAGTATATCACC+AGG	0.618786	7_2:-10316952	None:intergenic
AGTGGCACTTGAAACTTTAA+TGG	0.283958	7_2:+10316921	Msa1048550:CDS
ATATCTTGCTAAACTCTGAG+TGG	0.627445	7_2:+10316297	Msa1048550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGCTTAATGTTGTGAAAG+AGG	+	chr7_2:10316202-10316221	Msa1048550:CDS	30.0%
GATTCGATGAATCATATTGA+AGG	-	chr7_2:10316356-10316375	None:intergenic	30.0%
AGACCAATGAAAAATTTACC+TGG	-	chr7_2:10316413-10316432	None:intergenic	30.0%
TTTATCAAGTCACCATAAGT+AGG	-	chr7_2:10316837-10316856	None:intergenic	30.0%
AAAGCTTAATGTTGTGAAAG+AGG	+	chr7_2:10316202-10316221	Msa1048550:CDS	30.0%
GATTCGATGAATCATATTGA+AGG	-	chr7_2:10316356-10316375	None:intergenic	30.0%
AGACCAATGAAAAATTTACC+TGG	-	chr7_2:10316413-10316432	None:intergenic	30.0%
TTTATCAAGTCACCATAAGT+AGG	-	chr7_2:10316837-10316856	None:intergenic	30.0%
CTGAAACTCTCATCAAAAGA+TGG	-	chr7_2:10316014-10316033	None:intergenic	35.0%
ATCTACGAGTACATTGAGAA+AGG	+	chr7_2:10316122-10316141	Msa1048550:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	10315963	10317251	10315963	ID=Msa1048550;Name=Msa1048550
chr7_2	mRNA	10315963	10317251	10315963	ID=Msa1048550-mRNA-1;Parent=Msa1048550;Name=Msa1048550-mRNA-1;_AED=0.27;_eAED=0.28;_QI=0\|0\|0\|1\|1\|1\|2\|0\|249
chr7_2	exon	10315963	10316324	10315963	ID=Msa1048550-mRNA-1:exon:2734;Parent=Msa1048550-mRNA-1
chr7_2	exon	10316864	10317251	10316864	ID=Msa1048550-mRNA-1:exon:2735;Parent=Msa1048550-mRNA-1
chr7_2	CDS	10315963	10316324	10315963	ID=Msa1048550-mRNA-1:cds;Parent=Msa1048550-mRNA-1
chr7_2	CDS	10316864	10317251	10316864	ID=Msa1048550-mRNA-1:cds;Parent=Msa1048550-mRNA-1

Gene Sequence

>Msa1048550

ATGAACCTTGCTTATATTTTAAAACGGAGGGAGTATGAAGCAGAGGATCCATCTTTTGATGAGAGTTTCAGAAATGAAGTGAGAATATTATCAGAAATAAAGCACTGGCATATCGTGAAGCTTTATGGATTTTGCCTCCACAAAAGAATCATGTTTTTGATCTACGAGTACATTGAGAAAGGTAGCTTGTTCTCTGTCTTGTATGATGAAGTTGAAGCCGTTGAATTCAATTGGAGAAAAAAGCTTAATGTTGTGAAAGAGGTTGCATTTGGTCTATCATATCTTCATCATGATTGCACCCCTCCAATAGTGCATAGAGATGTATCTACTAGCAATATCTTGCTAAACTCTGAGTGGAAGCAAACTTGCATACACCATATGGTTGTGAGTGAAAAGTGTGATGTATATAGTTTTGGAGTAGTGGCACTTGAAACTTTAATGGGAAGACACCCTGGTGATATACTGTCATCACTTCAGTTAGCATCGACTCAAGGCATGAAATTATGTGAAGTCTTAGACCAGCGTCTCCCACTTCCAAATAATGTGAAGGTTTTACTTGAGATAATTCGTGTTGCTGTAGTAGCATTTGGATGCTTAAATCTCAACCCTTGCGCTCGACCAACAATGAAAGGTGTGTCACAGAGTTTTGTCACAGAGCTTACACCATTAAACATTCCGTTGAGTGAAATTTCAGTGCAGCAACTCATGAGTCATGAACTCAAAGCATTATTGTATATTGTAAACCTTTGA

Protein sequence

>Msa1048550

MNLAYILKRREYEAEDPSFDESFRNEVRILSEIKHWHIVKLYGFCLHKRIMFLIYEYIEKGSLFSVLYDEVEAVEFNWRKKLNVVKEVAFGLSYLHHDCTPPIVHRDVSTSNILLNSEWKQTCIHHMVVSEKCDVYSFGVVALETLMGRHPGDILSSLQLASTQGMKLCEVLDQRLPLPNNVKVLLEIIRVAVVAFGCLNLNPCARPTMKGVSQSFVTELTPLNIPLSEISVQQLMSHELKALLYIVNL*