AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1057880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1057880 MtrunA17_Chr3g0143461 70.455 132 20 2 50 162 246 377 8.93e-50 164
Msa1057880 MtrunA17_Chr1g0209041 91.045 67 6 0 4 70 250 316 3.90e-41 140
Msa1057880 MtrunA17_Chr1g0211401 49.550 111 38 1 69 161 316 426 7.82e-29 109
Msa1057880 MtrunA17_Chr5g0442111 68.889 45 5 1 26 70 24 59 1.22e-14 65.9
Msa1057880 MtrunA17_Chr1g0198451 39.726 73 44 0 83 155 348 420 6.53e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1057880 AT1G68200.1 74.286 70 15 1 4 70 196 265 6.58e-29 108
Msa1057880 AT1G68200.5 74.286 70 15 1 4 70 196 265 6.58e-29 108
Msa1057880 AT1G68200.2 73.913 69 15 1 5 70 196 264 3.95e-28 106
Msa1057880 AT1G68200.6 73.913 69 15 1 5 70 196 264 3.95e-28 106
Msa1057880 AT5G42210.1 50.450 111 37 1 68 160 322 432 4.73e-28 108
Msa1057880 AT1G66810.1 68.657 67 21 0 4 70 209 275 4.53e-26 100
Msa1057880 AT1G66810.3 68.657 67 21 0 4 70 209 275 4.53e-26 100
Msa1057880 AT2G16980.3 42.857 105 42 1 74 160 357 461 7.02e-22 91.3
Msa1057880 AT2G16980.2 42.857 105 42 1 74 160 342 446 8.04e-22 91.3
Msa1057880 AT2G16970.5 42.308 104 42 1 74 159 235 338 9.50e-22 90.1
Msa1057880 AT2G16970.2 42.308 104 42 1 74 159 342 445 1.45e-21 90.5
Msa1057880 AT2G16990.8 37.692 130 62 2 49 159 285 414 6.76e-18 80.1
Msa1057880 AT2G16990.1 37.692 130 62 2 49 159 312 441 8.03e-18 79.7
Msa1057880 AT2G16990.2 37.692 130 62 2 49 159 312 441 8.03e-18 79.7
Msa1057880 AT2G16990.7 38.596 114 51 2 49 143 312 425 3.59e-17 77.8
Msa1057880 AT2G16980.6 40.952 105 43 2 74 160 224 327 4.67e-17 77.0
Msa1057880 AT2G16980.4 40.952 105 43 2 74 160 357 460 4.77e-17 77.8
Msa1057880 AT5G42210.2 46.479 71 20 1 68 120 322 392 2.73e-11 60.8

Find 104 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAACCTCTCACATATTTA+AGG 0.154590 7_2:+40940197 None:intergenic
TTATAGATTGGGTTCTATAT+TGG 0.218702 7_2:-40940519 Msa1057880:three_prime_UTR
TCTTGAAAGATCCTCGTTAA+TGG 0.242729 7_2:+40941439 None:intergenic
TAGCGTGGGTGGCGGATCAT+TGG 0.277337 7_2:+40943050 None:intergenic
TATAGATTGGGTTCTATATT+GGG 0.279264 7_2:-40940518 Msa1057880:three_prime_UTR
CTGATAGGAGAAAGAGAAAA+AGG 0.299654 7_2:+40940271 None:intergenic
GATGGCCTAATAGTTGGATT+AGG 0.320977 7_2:+40940391 None:intergenic
ATGAAGCCAGGAAAATTAAA+AGG 0.324337 7_2:+40941968 None:intergenic
CTTGGCTTTCTCTTCATTAA+CGG 0.331892 7_2:-40942094 Msa1057880:CDS
CAACAGGCAGTGGCGGCATT+TGG 0.339764 7_2:+40941462 None:intergenic
GAAGAAGGAACACGATAATA+TGG 0.342760 7_2:-40941565 Msa1057880:three_prime_UTR
TATGTCCATCTAACTCAAAA+TGG 0.345856 7_2:+40941734 None:intergenic
GGAAATTCGCTCACGACATT+GGG 0.351212 7_2:-40943082 Msa1057880:CDS
GAACTTACCTAAATTCTATT+AGG 0.360006 7_2:+40941407 None:intergenic
GGCTTCATTATAATGTGTAT+TGG 0.364749 7_2:-40941953 Msa1057880:CDS
TTAGCTTACAATTAACTGAT+AGG 0.365879 7_2:+40940256 None:intergenic
ATGACATGATACTTATAGAT+TGG 0.369608 7_2:-40940531 Msa1057880:three_prime_UTR
TGTGCCCTAATCCAACTATT+AGG 0.379826 7_2:-40940396 Msa1057880:three_prime_UTR
AGGGCTCATGGTTGCATCTC+AGG 0.383344 7_2:-40942147 Msa1057880:CDS
GAAACTGGCACATGCCCTTA+TGG 0.383739 7_2:-40943113 Msa1057880:CDS
TTGGCTTTCTCTTCATTAAC+GGG 0.383799 7_2:-40942093 Msa1057880:intron
CACAAGACCTTTGCAATAGA+TGG 0.386099 7_2:+40940373 None:intergenic
AAGTTGTTAATGATTTCATT+AGG 0.396201 7_2:-40940453 Msa1057880:three_prime_UTR
TGTAACAAATGGCAGGAAAC+TGG 0.399409 7_2:-40943128 Msa1057880:CDS
TAGAGGGACAATGGGATGTT+TGG 0.404956 7_2:-40940689 Msa1057880:three_prime_UTR
GCAATAGATGGCCTAATAGT+TGG 0.413635 7_2:+40940385 None:intergenic
ACAGTATAGCATGGTCCTCT+TGG 0.414780 7_2:-40942744 Msa1057880:CDS
AATGCTAGGCATCTCATAGA+AGG 0.422742 7_2:+40941490 None:intergenic
TTCTTTCAGGGAAGGGCTCA+TGG 0.426994 7_2:-40942159 Msa1057880:intron
ATGGCCTAATAGTTGGATTA+GGG 0.430405 7_2:+40940392 None:intergenic
TGGAAATTCGCTCACGACAT+TGG 0.432744 7_2:-40943083 Msa1057880:CDS
TGCACATATTGTATCTCCCT+TGG 0.435532 7_2:-40942112 Msa1057880:CDS
AGCGTGGGTGGCGGATCATT+GGG 0.435554 7_2:+40943051 None:intergenic
ACTAATGAAAGCTACATTTG+AGG 0.437135 7_2:+40940083 None:intergenic
TATATGCATCTTTCGTCAAT+CGG 0.437649 7_2:-40940143 Msa1057880:three_prime_UTR
AGCTCTATTTCTATCTGAAA+AGG 0.445760 7_2:-40941993 Msa1057880:CDS
AGATCCTCGTTAATGGCAAC+AGG 0.447502 7_2:+40941446 None:intergenic
CAGTATAGCATGGTCCTCTT+GGG 0.447602 7_2:-40942743 Msa1057880:intron
GCATCTTTCGTCAATCGGTT+TGG 0.451112 7_2:-40940138 Msa1057880:three_prime_UTR
TAGGTTTGAGAGAATGACTA+TGG 0.451333 7_2:-40940434 Msa1057880:three_prime_UTR
TGACATGATACTTATAGATT+GGG 0.452945 7_2:-40940530 Msa1057880:three_prime_UTR
TCTGTCTTTCATTAGCAGCT+TGG 0.455797 7_2:+40940732 None:intergenic
TGTACGATGGTGTTTAAACT+TGG 0.459476 7_2:+40940819 None:intergenic
GGTTCTATATTGGGCAAAAG+AGG 0.465154 7_2:-40940509 Msa1057880:three_prime_UTR
TAATGTGTATTGGAATTGCT+TGG 0.466009 7_2:-40941943 Msa1057880:CDS
AATTTCCAGTGGTCTCCATA+AGG 0.470484 7_2:+40943098 None:intergenic
AGAACAAGCACAAATGTTAG+AGG 0.472788 7_2:-40940706 Msa1057880:three_prime_UTR
GAGTTGCATCAAGTTGAAGA+TGG 0.493345 7_2:-40940568 Msa1057880:three_prime_UTR
AACAGAGCAAACATACTTTG+TGG 0.497241 7_2:-40940221 Msa1057880:three_prime_UTR
TCGTTAATGGCAACAGGCAG+TGG 0.497673 7_2:+40941452 None:intergenic
TGCACCTATCACTGCAAAAG+TGG 0.498118 7_2:-40940647 Msa1057880:three_prime_UTR
AAAGTGGATATCTACAGCTT+TGG 0.498218 7_2:-40940631 Msa1057880:three_prime_UTR
AAAGTTTCTATAGATAAAGA+AGG 0.504229 7_2:+40941683 None:intergenic
TTTGAGAGAATGACTATGGT+TGG 0.507037 7_2:-40940430 Msa1057880:three_prime_UTR
GTTCTATATTGGGCAAAAGA+GGG 0.512150 7_2:-40940508 Msa1057880:three_prime_UTR
AATGTGTATTGGAATTGCTT+GGG 0.517681 7_2:-40941942 Msa1057880:intron
ATTTCCAGTGGTCTCCATAA+GGG 0.519626 7_2:+40943099 None:intergenic
ACAAATGTTAGAGGGACAAT+GGG 0.521045 7_2:-40940697 Msa1057880:three_prime_UTR
TGTTGCTATGCTGCAGCAAA+AGG 0.528129 7_2:-40943231 None:intergenic
AGACTGAGCTCTGTAACAAA+TGG 0.531908 7_2:-40943139 Msa1057880:CDS
CTTCATACTAAGAAAGAAGA+AGG 0.534068 7_2:-40941580 Msa1057880:three_prime_UTR
CATTAACAACTTCAACATCA+TGG 0.537170 7_2:+40940464 None:intergenic
CACAAATGTTAGAGGGACAA+TGG 0.540064 7_2:-40940698 Msa1057880:three_prime_UTR
GCTCAGTCTTGAACATCCCC+TGG 0.541107 7_2:+40943153 None:intergenic
ACAAGATGATCCCTGTGAGC+AGG 0.541778 7_2:-40942366 Msa1057880:intron
TGAACATGATACCTGCTCAC+AGG 0.545763 7_2:+40942355 None:intergenic
ATACACATTATAATGAAGCC+AGG 0.562281 7_2:+40941956 None:intergenic
TGAGCTCTGTAACAAATGGC+AGG 0.566245 7_2:-40943135 Msa1057880:CDS
AAAAGGTATTTGTGCGAGGA+GGG 0.574881 7_2:-40943214 Msa1057880:CDS
CAAGTTGAAGATGGAACAGA+AGG 0.575586 7_2:-40940559 Msa1057880:three_prime_UTR
TCCATCTAACTCAAAATGGA+AGG 0.576928 7_2:+40941738 None:intergenic
GGCATTTGGCTCATAATGCT+AGG 0.577053 7_2:+40941476 None:intergenic
TGGGATGTTTGGCACCAGAA+TGG 0.578599 7_2:-40940678 Msa1057880:three_prime_UTR
CTTTAATTGTGCTTCTGATG+AGG 0.580908 7_2:-40941658 Msa1057880:three_prime_UTR
TGTGCGAGGAGGGCGGAAAG+AGG 0.581667 7_2:-40943204 Msa1057880:CDS
TGAGATGGTTGTGTACAACC+AGG 0.582022 7_2:-40943171 Msa1057880:CDS
GTGGTCCTTAAATATGTGAG+AGG 0.591328 7_2:-40940202 Msa1057880:three_prime_UTR
AGAGGAAGAGCCTCTTGAGA+TGG 0.592690 7_2:-40943186 Msa1057880:CDS
CATGCCCTTATGGAGACCAC+TGG 0.593639 7_2:-40943103 Msa1057880:CDS
TCAGAGTATGATGCTTAGAG+TGG 0.595054 7_2:-40941763 Msa1057880:CDS
GAACATGATACCTGCTCACA+GGG 0.599005 7_2:+40942356 None:intergenic
TTCTATATTGGGCAAAAGAG+GGG 0.600711 7_2:-40940507 Msa1057880:three_prime_UTR
CAAAAGGTATTTGTGCGAGG+AGG 0.603500 7_2:-40943215 Msa1057880:CDS
GTGCTTCTGATGAGGAAGAA+AGG 0.605953 7_2:-40941650 Msa1057880:three_prime_UTR
TATTAGGCCATCTATTGCAA+AGG 0.612175 7_2:-40940380 Msa1057880:three_prime_UTR
TGTTCATTTACAGTATAGCA+TGG 0.612842 7_2:-40942753 Msa1057880:CDS
CGTTAATGAAGAGAAAGCCA+AGG 0.620962 7_2:+40942095 None:intergenic
GAACAAGCACAAATGTTAGA+GGG 0.627687 7_2:-40940705 Msa1057880:three_prime_UTR
CAGCAAAAGGTATTTGTGCG+AGG 0.628338 7_2:-40943218 Msa1057880:CDS
AGGTATTTGTGCGAGGAGGG+CGG 0.633035 7_2:-40943211 Msa1057880:CDS
GTTGAAGATGGAACAGAAGG+TGG 0.634859 7_2:-40940556 Msa1057880:three_prime_UTR
GAGATGGTTGTGTACAACCA+GGG 0.638365 7_2:-40943170 Msa1057880:CDS
TTGTACACAACCATCTCAAG+AGG 0.647753 7_2:+40943176 None:intergenic
TTAATGGCAACAGGCAGTGG+CGG 0.648108 7_2:+40941455 None:intergenic
GAAATTCGCTCACGACATTG+GGG 0.659026 7_2:-40943081 Msa1057880:CDS
TGTCGTGAGCGAATTTCCAG+TGG 0.672103 7_2:+40943087 None:intergenic
GAAGGAACACGATAATATGG+TGG 0.676501 7_2:-40941562 Msa1057880:three_prime_UTR
GTTAATGAAGAGAAAGCCAA+GGG 0.677516 7_2:+40942096 None:intergenic
TAGCTAAAGACATACCCAAG+AGG 0.693874 7_2:+40942729 None:intergenic
ACTGCCTGTTGCCATTAACG+AGG 0.709980 7_2:-40941450 Msa1057880:three_prime_UTR
CCACCCACGCTACAAAACTG+AGG 0.721664 7_2:-40943039 Msa1057880:intron
AGATGGTTGTGTACAACCAG+GGG 0.741581 7_2:-40943169 Msa1057880:CDS
GAATGACTATGGTTGGACTG+TGG 0.741878 7_2:-40940423 Msa1057880:three_prime_UTR
ATATGCAAGAAAGTGTACGA+TGG 0.782498 7_2:+40940806 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTTTGAATAACAAA+GGG + chr7_2:40940877-40940896 None:intergenic 10.0%
!!! AATTATATTTTGAATAACAA+AGG + chr7_2:40940878-40940897 None:intergenic 10.0%
!! TTATGCTGTATTAATTAATA+AGG - chr7_2:40940728-40940747 Msa1057880:three_prime_UTR 15.0%
!!! TTATATTTTGAATAACAAAG+GGG + chr7_2:40940876-40940895 None:intergenic 15.0%
!!! TATGCAAATCATTTTAAAAA+AGG + chr7_2:40942334-40942353 None:intergenic 15.0%
!!! TGTATATGATATTTTTTCAA+AGG + chr7_2:40942398-40942417 None:intergenic 15.0%
!!! AATTAAAGCATTTTTTCAAA+TGG - chr7_2:40942558-40942577 Msa1057880:intron 15.0%
!!! CTAATTTAGAATTTTTGTCT+TGG + chr7_2:40940519-40940538 None:intergenic 20.0%
!!! TAATGTGTAGTAACTTATTT+TGG - chr7_2:40940648-40940667 Msa1057880:three_prime_UTR 20.0%
!! TAATAAGGTAACATGTTTAT+AGG - chr7_2:40940743-40940762 Msa1057880:three_prime_UTR 20.0%
!!! AGTGGTAATATATTTTTGTT+AGG - chr7_2:40940992-40941011 Msa1057880:intron 20.0%
!!! GATTGAATGCTATTTATTTT+TGG - chr7_2:40941085-40941104 Msa1057880:intron 20.0%
!! CAGATTATAATCTATTAAGT+TGG - chr7_2:40941112-40941131 Msa1057880:intron 20.0%
!! AAGAAAGAATCATACAAATA+TGG + chr7_2:40941473-40941492 None:intergenic 20.0%
!! TTCTTTCTTGTCTAATTATA+TGG - chr7_2:40941484-40941503 Msa1057880:three_prime_UTR 20.0%
!! AAAGTTTCTATAGATAAAGA+AGG + chr7_2:40941633-40941652 None:intergenic 20.0%
!! ATTTCATTAAATAATGCACT+TGG + chr7_2:40942010-40942029 None:intergenic 20.0%
!! AATTAAATTGAAACATAGTG+TGG + chr7_2:40942069-40942088 None:intergenic 20.0%
!!! ATTGATTTACCTAATTAGTT+TGG - chr7_2:40942086-40942105 Msa1057880:intron 20.0%
!!! TTGAAATCTTATTTTGTAGA+AGG - chr7_2:40942716-40942735 Msa1057880:intron 20.0%
!! AAGTTGTTAATGATTTCATT+AGG - chr7_2:40942860-40942879 Msa1057880:intron 20.0%
!!! TAAGTCACCAAATATATTTT+CGG - chr7_2:40943003-40943022 Msa1057880:intron 20.0%
!! AAGAAAAACTCTGAAAAGTT+TGG + chr7_2:40940458-40940477 None:intergenic 25.0%
!! GAGTTTTTCTTTTTGCAATA+TGG - chr7_2:40940467-40940486 Msa1057880:three_prime_UTR 25.0%
! TAATACAGCATAAAATGAGA+AGG + chr7_2:40940721-40940740 None:intergenic 25.0%
! TTCCTCATTCTATATATCTT+TGG - chr7_2:40940829-40940848 Msa1057880:three_prime_UTR 25.0%
! AACCAAAGATATATAGAATG+AGG + chr7_2:40940834-40940853 None:intergenic 25.0%
!! AAGGGGAAAGTATATTTAAA+AGG + chr7_2:40940859-40940878 None:intergenic 25.0%
!! TTTTTGTTAGGCATACTAAA+AGG - chr7_2:40941004-40941023 Msa1057880:intron 25.0%
! TCACTTATCTTATTCTTTCA+GGG - chr7_2:40941142-40941161 Msa1057880:intron 25.0%
! AGAAATAAAAATAGAGAGCA+TGG + chr7_2:40941423-40941442 None:intergenic 25.0%
!!! GATTAATTTTTGTTTGCAGA+TGG - chr7_2:40941517-40941536 Msa1057880:three_prime_UTR 25.0%
!! CATTTTGCCTAATAGAATTT+AGG - chr7_2:40941899-40941918 Msa1057880:intron 25.0%
! GAACTTACCTAAATTCTATT+AGG + chr7_2:40941909-40941928 None:intergenic 25.0%
! ATAGAATTTAGGTAAGTTCT+TGG - chr7_2:40941910-40941929 Msa1057880:intron 25.0%
! GGACACAAAAAAAAAACAAA+TGG + chr7_2:40941989-40942008 None:intergenic 25.0%
! TTCAAACTTCCAAACTAATT+AGG + chr7_2:40942098-40942117 None:intergenic 25.0%
! CTAATTTCAACTTATTAGGA+AGG + chr7_2:40942148-40942167 None:intergenic 25.0%
! CTCACTAATTTCAACTTATT+AGG + chr7_2:40942152-40942171 None:intergenic 25.0%
!! CTAATAAGTTGAAATTAGTG+AGG - chr7_2:40942150-40942169 Msa1057880:CDS 25.0%
!!! ATTAGTGAGGTTTTTCAATT+TGG - chr7_2:40942163-40942182 Msa1057880:intron 25.0%
! ATAGTATGAGCTAATAATCT+CGG - chr7_2:40942223-40942242 Msa1057880:intron 25.0%
!! TGCATATTCATTATGCATTT+TGG - chr7_2:40942348-40942367 Msa1057880:intron 25.0%
! ATGACATGATACTTATAGAT+TGG - chr7_2:40942782-40942801 Msa1057880:intron 25.0%
! TGACATGATACTTATAGATT+GGG - chr7_2:40942783-40942802 Msa1057880:intron 25.0%
! TTATAGATTGGGTTCTATAT+TGG - chr7_2:40942794-40942813 Msa1057880:intron 25.0%
! TATAGATTGGGTTCTATATT+GGG - chr7_2:40942795-40942814 Msa1057880:intron 25.0%
!! AAGTCACCAAATATATTTTC+GGG - chr7_2:40943004-40943023 Msa1057880:intron 25.0%
! GAAAAACCCGAAAATATATT+TGG + chr7_2:40943013-40943032 None:intergenic 25.0%
!!! ATATATTTTCGGGTTTTTCA+TGG - chr7_2:40943014-40943033 Msa1057880:intron 25.0%
! TTAGCTTACAATTAACTGAT+AGG + chr7_2:40943060-40943079 None:intergenic 25.0%
TGTTCATTTACAGTATAGCA+TGG - chr7_2:40940560-40940579 Msa1057880:three_prime_UTR 30.0%
!!! GTAGTAACTTATTTTGGAAG+TGG - chr7_2:40940654-40940673 Msa1057880:three_prime_UTR 30.0%
AGGTATCATGTTCATAGTAT+TGG - chr7_2:40940967-40940986 Msa1057880:intron 30.0%
!! ATGTTCATAGTATTGGTTAG+TGG - chr7_2:40940974-40940993 Msa1057880:intron 30.0%
TACTAAAAGGAAGACATGAT+TGG - chr7_2:40941017-40941036 Msa1057880:intron 30.0%
CTCACTTATCTTATTCTTTC+AGG - chr7_2:40941141-40941160 Msa1057880:intron 30.0%
TTATCTTATTCTTTCAGGGA+AGG - chr7_2:40941146-40941165 Msa1057880:intron 30.0%
TATCTTATTCTTTCAGGGAA+GGG - chr7_2:40941147-40941166 Msa1057880:intron 30.0%
TAGAGCTGAAAAAAGAGAAT+GGG + chr7_2:40941307-40941326 None:intergenic 30.0%
ATAGAGCTGAAAAAAGAGAA+TGG + chr7_2:40941308-40941327 None:intergenic 30.0%
!! AGCTCTATTTCTATCTGAAA+AGG - chr7_2:40941320-40941339 Msa1057880:intron 30.0%
ATGAAGCCAGGAAAATTAAA+AGG + chr7_2:40941348-40941367 None:intergenic 30.0%
ATACACATTATAATGAAGCC+AGG + chr7_2:40941360-40941379 None:intergenic 30.0%
GGCTTCATTATAATGTGTAT+TGG - chr7_2:40941360-40941379 Msa1057880:intron 30.0%
TAATGTGTATTGGAATTGCT+TGG - chr7_2:40941370-40941389 Msa1057880:intron 30.0%
AATGTGTATTGGAATTGCTT+GGG - chr7_2:40941371-40941390 Msa1057880:intron 30.0%
TATGTCCATCTAACTCAAAA+TGG + chr7_2:40941582-40941601 None:intergenic 30.0%
CTTCATACTAAGAAAGAAGA+AGG - chr7_2:40941733-40941752 Msa1057880:exon 30.0%
AAATTGAAACATAGTGTGGT+TGG + chr7_2:40942065-40942084 None:intergenic 30.0%
ACTTATTAGGAAGGATAAAG+AGG + chr7_2:40942139-40942158 None:intergenic 30.0%
ATCTCATTCAGCCAAAAATA+TGG + chr7_2:40942205-40942224 None:intergenic 30.0%
CATTAACAACTTCAACATCA+TGG + chr7_2:40942852-40942871 None:intergenic 30.0%
GATAACCTCTCACATATTTA+AGG + chr7_2:40943119-40943138 None:intergenic 30.0%
TATATGCATCTTTCGTCAAT+CGG - chr7_2:40943170-40943189 Msa1057880:CDS 30.0%
CTTGTTCAGTAAGTGAATGA+CGG + chr7_2:40940355-40940374 None:intergenic 35.0%
! AAACTCTGAAAAGTTTGGCA+GGG + chr7_2:40940453-40940472 None:intergenic 35.0%
! AAAACTCTGAAAAGTTTGGC+AGG + chr7_2:40940454-40940473 None:intergenic 35.0%
GTTAATGAAGAGAAAGCCAA+GGG + chr7_2:40941220-40941239 None:intergenic 35.0%
! CTTGGCTTTCTCTTCATTAA+CGG - chr7_2:40941219-40941238 Msa1057880:intron 35.0%
! TTGGCTTTCTCTTCATTAAC+GGG - chr7_2:40941220-40941239 Msa1057880:intron 35.0%
CTGTAGTAATGTGAACATGA+TGG + chr7_2:40941267-40941286 None:intergenic 35.0%
!!! AAGGCACCTTTTAATTTTCC+TGG - chr7_2:40941339-40941358 Msa1057880:intron 35.0%
!!! TCCTTCCATTTTGAGTTAGA+TGG - chr7_2:40941574-40941593 Msa1057880:three_prime_UTR 35.0%
TCCATCTAACTCAAAATGGA+AGG + chr7_2:40941578-40941597 None:intergenic 35.0%
CTTTAATTGTGCTTCTGATG+AGG - chr7_2:40941655-40941674 Msa1057880:three_prime_UTR 35.0%
GAAGAAGGAACACGATAATA+TGG - chr7_2:40941748-40941767 Msa1057880:CDS 35.0%
TCTTGAAAGATCCTCGTTAA+TGG + chr7_2:40941877-40941896 None:intergenic 35.0%
! TTCAAGTGCAGCCATATTTT+TGG - chr7_2:40942191-40942210 Msa1057880:intron 35.0%
!! AGCAGTATTTTTGATCTAGC+AGG - chr7_2:40942446-40942465 Msa1057880:intron 35.0%
TGTACGATGGTGTTTAAACT+TGG + chr7_2:40942497-40942516 None:intergenic 35.0%
! ATATGCAAGAAAGTGTACGA+TGG + chr7_2:40942510-40942529 None:intergenic 35.0%
AGAACAAGCACAAATGTTAG+AGG - chr7_2:40942607-40942626 Msa1057880:intron 35.0%
GAACAAGCACAAATGTTAGA+GGG - chr7_2:40942608-40942627 Msa1057880:intron 35.0%
ACAAATGTTAGAGGGACAAT+GGG - chr7_2:40942616-40942635 Msa1057880:intron 35.0%
!! ATATCCACTTTTGCAGTGAT+AGG + chr7_2:40942673-40942692 None:intergenic 35.0%
AAAGTGGATATCTACAGCTT+TGG - chr7_2:40942682-40942701 Msa1057880:intron 35.0%
GTTCTATATTGGGCAAAAGA+GGG - chr7_2:40942805-40942824 Msa1057880:intron 35.0%
TTCTATATTGGGCAAAAGAG+GGG - chr7_2:40942806-40942825 Msa1057880:intron 35.0%
TAGGTTTGAGAGAATGACTA+TGG - chr7_2:40942879-40942898 Msa1057880:intron 35.0%
TTTGAGAGAATGACTATGGT+TGG - chr7_2:40942883-40942902 Msa1057880:intron 35.0%
ATGGCCTAATAGTTGGATTA+GGG + chr7_2:40942924-40942943 None:intergenic 35.0%
TATTAGGCCATCTATTGCAA+AGG - chr7_2:40942933-40942952 Msa1057880:intron 35.0%
CTGATAGGAGAAAGAGAAAA+AGG + chr7_2:40943045-40943064 None:intergenic 35.0%
AACAGAGCAAACATACTTTG+TGG - chr7_2:40943092-40943111 Msa1057880:CDS 35.0%
!! AAAAGGTATTTGTGCGAGGA+GGG - chr7_2:40940099-40940118 Msa1057880:three_prime_UTR 40.0%
TTGTACACAACCATCTCAAG+AGG + chr7_2:40940140-40940159 None:intergenic 40.0%
AGACTGAGCTCTGTAACAAA+TGG - chr7_2:40940174-40940193 Msa1057880:three_prime_UTR 40.0%
TGTAACAAATGGCAGGAAAC+TGG - chr7_2:40940185-40940204 Msa1057880:three_prime_UTR 40.0%
ATTTCCAGTGGTCTCCATAA+GGG + chr7_2:40940217-40940236 None:intergenic 40.0%
AATTTCCAGTGGTCTCCATA+AGG + chr7_2:40940218-40940237 None:intergenic 40.0%
AAATGGCATCTATGGCCATA+CGG + chr7_2:40940332-40940351 None:intergenic 40.0%
! AATGACGGAAATGGCATCTA+TGG + chr7_2:40940340-40940359 None:intergenic 40.0%
! CAGTAAGTGAATGACGGAAA+TGG + chr7_2:40940349-40940368 None:intergenic 40.0%
TAGCTAAAGACATACCCAAG+AGG + chr7_2:40940587-40940606 None:intergenic 40.0%
TGAGGAATGAAACTCACACA+CGG + chr7_2:40940816-40940835 None:intergenic 40.0%
GGAACACAGTCAACTTCTAA+TGG - chr7_2:40941038-40941057 Msa1057880:intron 40.0%
TGCACATATTGTATCTCCCT+TGG - chr7_2:40941201-40941220 Msa1057880:intron 40.0%
CGTTAATGAAGAGAAAGCCA+AGG + chr7_2:40941221-40941240 None:intergenic 40.0%
TCAGAGTATGATGCTTAGAG+TGG - chr7_2:40941550-40941569 Msa1057880:three_prime_UTR 40.0%
GAAGGAACACGATAATATGG+TGG - chr7_2:40941751-40941770 Msa1057880:CDS 40.0%
AATGCTAGGCATCTCATAGA+AGG + chr7_2:40941826-40941845 None:intergenic 40.0%
TCTGTCTTTCATTAGCAGCT+TGG + chr7_2:40942584-40942603 None:intergenic 40.0%
CACAAATGTTAGAGGGACAA+TGG - chr7_2:40942615-40942634 Msa1057880:intron 40.0%
!! GAGTTGCATCAAGTTGAAGA+TGG - chr7_2:40942745-40942764 Msa1057880:CDS 40.0%
! CAAGTTGAAGATGGAACAGA+AGG - chr7_2:40942754-40942773 Msa1057880:CDS 40.0%
GGTTCTATATTGGGCAAAAG+AGG - chr7_2:40942804-40942823 Msa1057880:intron 40.0%
TGTGCCCTAATCCAACTATT+AGG - chr7_2:40942917-40942936 Msa1057880:intron 40.0%
GATGGCCTAATAGTTGGATT+AGG + chr7_2:40942925-40942944 None:intergenic 40.0%
GCAATAGATGGCCTAATAGT+TGG + chr7_2:40942931-40942950 None:intergenic 40.0%
CACAAGACCTTTGCAATAGA+TGG + chr7_2:40942943-40942962 None:intergenic 40.0%
GTGGTCCTTAAATATGTGAG+AGG - chr7_2:40943111-40943130 Msa1057880:CDS 40.0%
!! CAGCAAAAGGTATTTGTGCG+AGG - chr7_2:40940095-40940114 Msa1057880:three_prime_UTR 45.0%
!! CAAAAGGTATTTGTGCGAGG+AGG - chr7_2:40940098-40940117 Msa1057880:three_prime_UTR 45.0%
TGAGATGGTTGTGTACAACC+AGG - chr7_2:40940142-40940161 Msa1057880:three_prime_UTR 45.0%
GAGATGGTTGTGTACAACCA+GGG - chr7_2:40940143-40940162 Msa1057880:three_prime_UTR 45.0%
AGATGGTTGTGTACAACCAG+GGG - chr7_2:40940144-40940163 Msa1057880:three_prime_UTR 45.0%
TGAGCTCTGTAACAAATGGC+AGG - chr7_2:40940178-40940197 Msa1057880:three_prime_UTR 45.0%
TGGAAATTCGCTCACGACAT+TGG - chr7_2:40940230-40940249 Msa1057880:three_prime_UTR 45.0%
GGAAATTCGCTCACGACATT+GGG - chr7_2:40940231-40940250 Msa1057880:three_prime_UTR 45.0%
GAAATTCGCTCACGACATTG+GGG - chr7_2:40940232-40940251 Msa1057880:three_prime_UTR 45.0%
!! AGACCTCAGTTTTGTAGCGT+GGG + chr7_2:40940280-40940299 None:intergenic 45.0%
AATGGCATCTATGGCCATAC+GGG + chr7_2:40940331-40940350 None:intergenic 45.0%
ACAGTATAGCATGGTCCTCT+TGG - chr7_2:40940569-40940588 Msa1057880:three_prime_UTR 45.0%
CAGTATAGCATGGTCCTCTT+GGG - chr7_2:40940570-40940589 Msa1057880:three_prime_UTR 45.0%
GAGAACATTGCAGCAGCATA+AGG + chr7_2:40940771-40940790 None:intergenic 45.0%
GAACATGATACCTGCTCACA+GGG + chr7_2:40940960-40940979 None:intergenic 45.0%
TGAACATGATACCTGCTCAC+AGG + chr7_2:40940961-40940980 None:intergenic 45.0%
! TCTCTTCATTAACGGGTGAG+TGG - chr7_2:40941227-40941246 Msa1057880:intron 45.0%
GTGCTTCTGATGAGGAAGAA+AGG - chr7_2:40941663-40941682 Msa1057880:three_prime_UTR 45.0%
! GGCATTTGGCTCATAATGCT+AGG + chr7_2:40941840-40941859 None:intergenic 45.0%
AGATCCTCGTTAATGGCAAC+AGG + chr7_2:40941870-40941889 None:intergenic 45.0%
! GATAAAGAGGTGTGACAGCA+AGG + chr7_2:40942126-40942145 None:intergenic 45.0%
TCACTGTCGAGAAAGATGCT+CGG + chr7_2:40942293-40942312 None:intergenic 45.0%
TAGAGGGACAATGGGATGTT+TGG - chr7_2:40942624-40942643 Msa1057880:intron 45.0%
!! GGTGCATTTTTGAGCCATTC+TGG + chr7_2:40942652-40942671 None:intergenic 45.0%
TGCACCTATCACTGCAAAAG+TGG - chr7_2:40942666-40942685 Msa1057880:intron 45.0%
GTTGAAGATGGAACAGAAGG+TGG - chr7_2:40942757-40942776 Msa1057880:CDS 45.0%
GAATGACTATGGTTGGACTG+TGG - chr7_2:40942890-40942909 Msa1057880:intron 45.0%
GCATCTTTCGTCAATCGGTT+TGG - chr7_2:40943175-40943194 Msa1057880:CDS 45.0%
AGAGGAAGAGCCTCTTGAGA+TGG - chr7_2:40940127-40940146 Msa1057880:three_prime_UTR 50.0%
GAAACTGGCACATGCCCTTA+TGG - chr7_2:40940200-40940219 Msa1057880:three_prime_UTR 50.0%
TGTCGTGAGCGAATTTCCAG+TGG + chr7_2:40940229-40940248 None:intergenic 50.0%
!! CAGACCTCAGTTTTGTAGCG+TGG + chr7_2:40940281-40940300 None:intergenic 50.0%
GCTACAAAACTGAGGTCTGC+AGG - chr7_2:40940282-40940301 Msa1057880:three_prime_UTR 50.0%
CAAAACTGAGGTCTGCAGGA+TGG - chr7_2:40940286-40940305 Msa1057880:three_prime_UTR 50.0%
ACAAGATGATCCCTGTGAGC+AGG - chr7_2:40940947-40940966 Msa1057880:intron 50.0%
TTCTTTCAGGGAAGGGCTCA+TGG - chr7_2:40941154-40941173 Msa1057880:intron 50.0%
TTAATGGCAACAGGCAGTGG+CGG + chr7_2:40941861-40941880 None:intergenic 50.0%
TCGTTAATGGCAACAGGCAG+TGG + chr7_2:40941864-40941883 None:intergenic 50.0%
ACTGCCTGTTGCCATTAACG+AGG - chr7_2:40941863-40941882 Msa1057880:intron 50.0%
TGGGATGTTTGGCACCAGAA+TGG - chr7_2:40942635-40942654 Msa1057880:intron 50.0%
AGGTATTTGTGCGAGGAGGG+CGG - chr7_2:40940102-40940121 Msa1057880:three_prime_UTR 55.0%
GCTCAGTCTTGAACATCCCC+TGG + chr7_2:40940163-40940182 None:intergenic 55.0%
CATGCCCTTATGGAGACCAC+TGG - chr7_2:40940210-40940229 Msa1057880:three_prime_UTR 55.0%
!! CAGTTTTGTAGCGTGGGTGG+CGG + chr7_2:40940274-40940293 None:intergenic 55.0%
!! CCTCAGTTTTGTAGCGTGGG+TGG + chr7_2:40940277-40940296 None:intergenic 55.0%
CCACCCACGCTACAAAACTG+AGG - chr7_2:40940274-40940293 Msa1057880:three_prime_UTR 55.0%
TCTGCAGGATGGTCCTTGCT+GGG - chr7_2:40940297-40940316 Msa1057880:three_prime_UTR 55.0%
AGGGCTCATGGTTGCATCTC+AGG - chr7_2:40941166-40941185 Msa1057880:intron 55.0%
! CGAGAAAGATGCTCGGTGAG+TGG + chr7_2:40942286-40942305 None:intergenic 55.0%
!! AGCGTGGGTGGCGGATCATT+GGG + chr7_2:40940265-40940284 None:intergenic 60.0%
!! TAGCGTGGGTGGCGGATCAT+TGG + chr7_2:40940266-40940285 None:intergenic 60.0%
GTCTGCAGGATGGTCCTTGC+TGG - chr7_2:40940296-40940315 Msa1057880:three_prime_UTR 60.0%
CTGCAGGATGGTCCTTGCTG+GGG - chr7_2:40940298-40940317 Msa1057880:three_prime_UTR 60.0%
CAACAGGCAGTGGCGGCATT+TGG + chr7_2:40941854-40941873 None:intergenic 60.0%
! GATGCTCGGTGAGTGGTGAC+AGG + chr7_2:40942279-40942298 None:intergenic 60.0%
TGTGCGAGGAGGGCGGAAAG+AGG - chr7_2:40940109-40940128 Msa1057880:three_prime_UTR 65.0%
ACGGGCACACAACCCCAGCA+AGG + chr7_2:40940313-40940332 None:intergenic 65.0%
GCTGGGGTTGTGTGCCCGTA+TGG - chr7_2:40940314-40940333 Msa1057880:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 40940089 40943246 40940089 ID=Msa1057880;Name=Msa1057880
chr7_2 mRNA 40940089 40943246 40940089 ID=Msa1057880-mRNA-1;Parent=Msa1057880;Name=Msa1057880-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0.83|0.85|1|0.83|0.71|7|1107|162
chr7_2 exon 40940089 40940867 40940089 ID=Msa1057880-mRNA-1:exon:7493;Parent=Msa1057880-mRNA-1
chr7_2 exon 40941415 40941799 40941415 ID=Msa1057880-mRNA-1:exon:7492;Parent=Msa1057880-mRNA-1
chr7_2 exon 40941943 40942013 40941943 ID=Msa1057880-mRNA-1:exon:7491;Parent=Msa1057880-mRNA-1
chr7_2 exon 40942094 40942172 40942094 ID=Msa1057880-mRNA-1:exon:7490;Parent=Msa1057880-mRNA-1
chr7_2 exon 40942367 40942408 40942367 ID=Msa1057880-mRNA-1:exon:7489;Parent=Msa1057880-mRNA-1
chr7_2 exon 40942744 40942776 40942744 ID=Msa1057880-mRNA-1:exon:7488;Parent=Msa1057880-mRNA-1
chr7_2 exon 40943040 40943246 40943040 ID=Msa1057880-mRNA-1:exon:7487;Parent=Msa1057880-mRNA-1
chr7_2 CDS 40943040 40943246 40943040 ID=Msa1057880-mRNA-1:cds;Parent=Msa1057880-mRNA-1
chr7_2 CDS 40942744 40942776 40942744 ID=Msa1057880-mRNA-1:cds;Parent=Msa1057880-mRNA-1
chr7_2 CDS 40942367 40942408 40942367 ID=Msa1057880-mRNA-1:cds;Parent=Msa1057880-mRNA-1
chr7_2 CDS 40942094 40942172 40942094 ID=Msa1057880-mRNA-1:cds;Parent=Msa1057880-mRNA-1
chr7_2 CDS 40941943 40942013 40941943 ID=Msa1057880-mRNA-1:cds;Parent=Msa1057880-mRNA-1
chr7_2 CDS 40941743 40941799 40941743 ID=Msa1057880-mRNA-1:cds;Parent=Msa1057880-mRNA-1
chr7_2 three_prime_UTR 40941415 40941742 40941415 ID=Msa1057880-mRNA-1:three_prime_utr;Parent=Msa1057880-mRNA-1
chr7_2 three_prime_UTR 40940089 40940867 40940089 ID=Msa1057880-mRNA-1:three_prime_utr;Parent=Msa1057880-mRNA-1
Gene Sequence

>Msa1057880

ATGCTGCAGCAAAAGGTATTTGTGCGAGGAGGGCGGAAAGAGGAAGAGCCTCTTGAGATGGTTGTGTACAACCAGGGGATGTTCAAGACTGAGCTCTGTAACAAATGGCAGGAAACTGGCACATGCCCTTATGGAGACCACTGGAAATTCGCTCACGACATTGGGGAGCTTCGCCCAATGATCCGCCACCCACGCTACAAAACTGAGATGTTCATTTACAGTATAGCATGGTCCTCTTGGATACGAAGTATTGTTTCTAAACAAGATGATCCCTGTGAGCAGGGAAGGGCTCATGGTTGCATCTCAGGCATATGTTCGATTGCACATATTGTATCTCCCTTGGCTTTCTCTTCATTAACGGCTCTATTTCTATCTGAAAAGGCACCTTTTAATTTTCCTGGCTTCATTATAATGTGTATTGGAATTGCTTGGATGGTATCTTTTGTTCAGAGTATGATGCTTAGAGTGGTTCCTTCCATTTTGAGTTAG
Protein sequence

>Msa1057880

MLQQKVFVRGGRKEEEPLEMVVYNQGMFKTELCNKWQETGTCPYGDHWKFAHDIGELRPMIRHPRYKTEMFIYSIAWSSWIRSIVSKQDDPCEQGRAHGCISGICSIAHIVSPLAFSSLTALFLSEKAPFNFPGFIIMCIGIAWMVSFVQSMMLRVVPSILS*