AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300010 MtrunA17_Chr1g0209041 91.837 98 8 0 4 101 250 347 9.09e-66 205
Msa0300010 MtrunA17_Chr5g0442111 78.667 75 7 1 26 100 24 89 4.79e-37 123
Msa0300010 MtrunA17_Chr3g0143461 94.030 67 4 0 113 179 311 377 1.80e-34 124
Msa0300010 MtrunA17_Chr1g0211401 60.938 64 25 0 115 178 363 426 5.15e-20 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300010 AT1G68200.1 76.768 99 20 1 4 99 196 294 3.21e-50 164
Msa0300010 AT1G68200.5 76.768 99 20 1 4 99 196 294 3.21e-50 164
Msa0300010 AT1G68200.2 76.531 98 20 1 5 99 196 293 2.16e-49 162
Msa0300010 AT1G68200.6 76.531 98 20 1 5 99 196 293 2.16e-49 162
Msa0300010 AT1G66810.1 70.213 94 28 0 4 97 209 302 2.93e-45 151
Msa0300010 AT1G66810.3 70.213 94 28 0 4 97 209 302 2.93e-45 151
Msa0300010 AT5G42210.1 60.000 65 26 0 113 177 368 432 1.35e-19 85.5
Msa0300010 AT2G16980.3 52.308 65 31 0 113 177 397 461 3.75e-17 78.6
Msa0300010 AT2G16980.2 52.308 65 31 0 113 177 382 446 4.40e-17 78.2
Msa0300010 AT2G16970.5 51.562 64 31 0 113 176 275 338 4.08e-16 75.1
Msa0300010 AT2G16970.2 51.562 64 31 0 113 176 382 445 4.39e-16 75.5
Msa0300010 AT2G16980.4 49.231 65 32 1 113 177 397 460 1.59e-12 65.1
Msa0300010 AT2G16980.6 49.231 65 32 1 113 177 264 327 1.87e-12 64.7
Msa0300010 AT2G16990.1 48.438 64 33 0 113 176 378 441 6.51e-12 63.5
Msa0300010 AT2G16990.2 48.438 64 33 0 113 176 378 441 6.51e-12 63.5
Msa0300010 AT2G16990.8 48.438 64 33 0 113 176 351 414 6.86e-12 63.2
Msa0300010 AT2G16990.7 54.167 48 22 0 113 160 378 425 4.04e-11 61.2

Find 113 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAACCTCTCACATATTTA+AGG 0.143428 2_3:-55339780 None:intergenic
GTCTGCAGGATGGTCCTTGC+TGG 0.159266 2_3:+55336836 Msa0300010:CDS
TTATAGATTGGGTTCTATAT+TGG 0.218702 2_3:+55339333 Msa0300010:three_prime_UTR
TCTTGAAAGATCCTCGTTAA+TGG 0.242729 2_3:-55338414 None:intergenic
TATAGATTGGGTTCTATATT+GGG 0.279264 2_3:+55339334 Msa0300010:three_prime_UTR
CTGATAGGAGAAAGAGAAAA+AGG 0.299654 2_3:-55339706 None:intergenic
GATGGCCTAATAGTTGGATT+AGG 0.320977 2_3:-55339501 None:intergenic
ATGAAGCCAGGAAAATTAAA+AGG 0.324337 2_3:-55337885 None:intergenic
CTTGGCTTTCTCTTCATTAA+CGG 0.331892 2_3:+55337759 Msa0300010:CDS
TAGCGTGGGTGGCAGATCAT+TGG 0.336562 2_3:-55336803 None:intergenic
CAACAGGCAGTGGCGGCATT+TGG 0.339764 2_3:-55338391 None:intergenic
TGCAACACCTTTGCAATAGA+TGG 0.340385 2_3:-55339519 None:intergenic
GAAGAAGGAACACGATAATA+TGG 0.342760 2_3:+55338288 Msa0300010:three_prime_UTR
TATGTCCATCTAACTCAAAA+TGG 0.345856 2_3:-55338119 None:intergenic
GAACTTACCTAAATTCTATT+AGG 0.360006 2_3:-55338446 None:intergenic
GTAAATTCGCTCACGACATT+GGG 0.363687 2_3:+55336771 Msa0300010:CDS
GGCTTCATTATAATGTGTAT+TGG 0.364749 2_3:+55337900 Msa0300010:CDS
TTAGCTTACAATTAACTGAT+AGG 0.365879 2_3:-55339721 None:intergenic
CATGACGGAAATGGCATCTA+TGG 0.367595 2_3:-55336877 None:intergenic
ATGACATGATACTTATAGAT+TGG 0.369608 2_3:+55339321 Msa0300010:three_prime_UTR
TGTGCCCTAATCCAACTATT+AGG 0.379826 2_3:+55339496 Msa0300010:three_prime_UTR
AGGGCTCATGGTTGCATCTC+AGG 0.383344 2_3:+55337706 Msa0300010:intron
GAAACTGGCACATGCCCTTA+TGG 0.383739 2_3:+55336740 Msa0300010:CDS
TTGGCTTTCTCTTCATTAAC+GGG 0.383799 2_3:+55337760 Msa0300010:CDS
AGTGTTCCACCATTGTTTGA+TGG 0.394571 2_3:+55339567 Msa0300010:three_prime_UTR
AAGTTGTTAATGATTTCATT+AGG 0.396201 2_3:+55339439 Msa0300010:three_prime_UTR
TGTAACAAATGGCAGGAAAC+TGG 0.399409 2_3:+55336725 Msa0300010:CDS
TCTGCAGGATGGTCCTTGCT+GGG 0.402584 2_3:+55336837 Msa0300010:CDS
TAGAGGGACAATGGGATGTT+TGG 0.404956 2_3:+55339163 Msa0300010:three_prime_UTR
TTCTTTCTGGGAAGGGCTCA+TGG 0.408059 2_3:+55337694 Msa0300010:intron
GCAATAGATGGCCTAATAGT+TGG 0.413635 2_3:-55339507 None:intergenic
CTTTAATTGTGCTTCTGATA+AGG 0.414620 2_3:+55338195 Msa0300010:three_prime_UTR
CAGTAAGTGCATGACGGAAA+TGG 0.414882 2_3:-55336886 None:intergenic
ACAAAATAAAGAGCAAGAAT+TGG 0.422434 2_3:-55339603 None:intergenic
AATGCCAGGCATCTCATAGA+AGG 0.427520 2_3:-55338363 None:intergenic
ATGGCCTAATAGTTGGATTA+GGG 0.430405 2_3:-55339500 None:intergenic
TGCACATATTGTATCTCCCT+TGG 0.435532 2_3:+55337741 Msa0300010:CDS
TGTAAATTCGCTCACGACAT+TGG 0.444174 2_3:+55336770 Msa0300010:CDS
AGCTCTATTTCTATCTGAAA+AGG 0.445760 2_3:+55337860 Msa0300010:intron
AGATCCTCGTTAATGGCAAC+AGG 0.447502 2_3:-55338407 None:intergenic
TAGGTTTGAGAGAATGACTA+TGG 0.451333 2_3:+55339458 Msa0300010:three_prime_UTR
TGACATGATACTTATAGATT+GGG 0.452945 2_3:+55339322 Msa0300010:three_prime_UTR
TCTGTCTTTCATTAGCAGCT+TGG 0.455797 2_3:-55339120 None:intergenic
AGCGTGGGTGGCAGATCATT+GGG 0.457762 2_3:-55336802 None:intergenic
TGTACGATGGTGTTTAAACT+TGG 0.459476 2_3:-55339033 None:intergenic
GGTTCTATATTGGGCAAAAG+AGG 0.465154 2_3:+55339343 Msa0300010:three_prime_UTR
AATTTACAGTGGTCTCCATA+AGG 0.465847 2_3:-55336755 None:intergenic
TAATGTGTATTGGAATTGCT+TGG 0.466009 2_3:+55337910 Msa0300010:CDS
AGAACAAGCACAAATGTTAG+AGG 0.472788 2_3:+55339146 Msa0300010:three_prime_UTR
CATTAACAACTTCAACATCA+TGG 0.488816 2_3:-55339428 None:intergenic
GAGTTGCATCAAGTTGAAGA+TGG 0.493345 2_3:+55339284 Msa0300010:three_prime_UTR
TATTCCTTCTATGAGATGCC+TGG 0.496545 2_3:+55338359 Msa0300010:three_prime_UTR
AACAGAGCAAACATACTTTG+TGG 0.497241 2_3:+55339756 Msa0300010:three_prime_UTR
TCGTTAATGGCAACAGGCAG+TGG 0.497673 2_3:-55338401 None:intergenic
TGCACCTATCACTGCAAAAG+TGG 0.498118 2_3:+55339205 Msa0300010:three_prime_UTR
AAAGTGGATATCTACAGCTT+TGG 0.498218 2_3:+55339221 Msa0300010:three_prime_UTR
AAAGTTTCTATAGATAAAGA+AGG 0.504229 2_3:-55338170 None:intergenic
GGCATTTGGCTCATAATGCC+AGG 0.504404 2_3:-55338377 None:intergenic
TTTGAGAGAATGACTATGGT+TGG 0.507037 2_3:+55339462 Msa0300010:three_prime_UTR
AAATGGCATCTATGGCCATA+CGG 0.511220 2_3:-55336869 None:intergenic
GTTCTATATTGGGCAAAAGA+GGG 0.512150 2_3:+55339344 Msa0300010:three_prime_UTR
GCTGGGGTTGTGTGCCCGTA+TGG 0.512898 2_3:+55336854 Msa0300010:CDS
AATGTGTATTGGAATTGCTT+GGG 0.517681 2_3:+55337911 Msa0300010:CDS
ACAAATGTTAGAGGGACAAT+GGG 0.521045 2_3:+55339155 Msa0300010:three_prime_UTR
GCTACAAAACTGAGGTCTGC+AGG 0.527724 2_3:+55336822 Msa0300010:CDS
ATTTACAGTGGTCTCCATAA+GGG 0.527756 2_3:-55336754 None:intergenic
TGTTGCTATGCTGCAGCAAA+AGG 0.528129 2_3:+55336622 None:intergenic
AGACTGAGCTCTGTAACAAA+TGG 0.531908 2_3:+55336714 Msa0300010:CDS
AATGGCATCTATGGCCATAC+GGG 0.531942 2_3:-55336868 None:intergenic
CTTCATACTAAGAAAGAAGA+AGG 0.534068 2_3:+55338273 Msa0300010:three_prime_UTR
CACAAATGTTAGAGGGACAA+TGG 0.540064 2_3:+55339154 Msa0300010:three_prime_UTR
GCTCAGTCTTGAACATCCCC+TGG 0.541107 2_3:-55336700 None:intergenic
ACAAGATGATCCCTGTGAGC+AGG 0.541778 2_3:+55337487 Msa0300010:CDS
TGAACATGATACCTGCTCAC+AGG 0.545763 2_3:-55337498 None:intergenic
GTGCTTCTGATAAGGAAGAA+AGG 0.547745 2_3:+55338203 Msa0300010:three_prime_UTR
TGCAAAGGTGTTGCAGATGT+CGG 0.550893 2_3:+55339527 Msa0300010:three_prime_UTR
ATACACATTATAATGAAGCC+AGG 0.562281 2_3:-55337897 None:intergenic
TGAGCTCTGTAACAAATGGC+AGG 0.566245 2_3:+55336718 Msa0300010:CDS
CTGCAGGATGGTCCTTGCTG+GGG 0.572424 2_3:+55336838 Msa0300010:CDS
AAAAGGTATTTGTGCGAGGA+GGG 0.574881 2_3:+55336639 Msa0300010:CDS
CAAGTTGAAGATGGAACAGA+AGG 0.575586 2_3:+55339293 Msa0300010:three_prime_UTR
TCCATCTAACTCAAAATGGA+AGG 0.576928 2_3:-55338115 None:intergenic
TGGGATGTTTGGCACCAGAA+TGG 0.578599 2_3:+55339174 Msa0300010:three_prime_UTR
TGTGCGAGGAGGGCGGAAAG+AGG 0.581667 2_3:+55336649 Msa0300010:CDS
TGAGATGGTTGTGTACAACC+AGG 0.582022 2_3:+55336682 Msa0300010:CDS
AGAGGAAGAGCCTCTTGAGA+TGG 0.592690 2_3:+55336667 Msa0300010:CDS
TCAGAGTATGATGCTTAGAG+TGG 0.595054 2_3:+55338090 Msa0300010:CDS
GAACATGATACCTGCTCACA+GGG 0.599005 2_3:-55337497 None:intergenic
TTCTATATTGGGCAAAAGAG+GGG 0.600711 2_3:+55339345 Msa0300010:three_prime_UTR
CAAAAGGTATTTGTGCGAGG+AGG 0.603500 2_3:+55336638 Msa0300010:CDS
CTTGTTCAGTAAGTGCATGA+CGG 0.608476 2_3:-55336892 None:intergenic
CAAAACTGAGGTCTGCAGGA+TGG 0.616724 2_3:+55336826 Msa0300010:CDS
AAGGTGTTGCAGATGTCGGA+AGG 0.619686 2_3:+55339531 Msa0300010:three_prime_UTR
CGTTAATGAAGAGAAAGCCA+AGG 0.620962 2_3:-55337758 None:intergenic
TATTAGGCCATCTATTGCAA+AGG 0.622506 2_3:+55339512 Msa0300010:three_prime_UTR
GTGGTCCTTAAATATGTGAG+AGG 0.625201 2_3:+55339775 Msa0300010:three_prime_UTR
ACGGGCACACAACCCCAGCA+AGG 0.626804 2_3:-55336850 None:intergenic
GAACAAGCACAAATGTTAGA+GGG 0.627687 2_3:+55339147 Msa0300010:three_prime_UTR
CAGCAAAAGGTATTTGTGCG+AGG 0.628338 2_3:+55336635 Msa0300010:CDS
AGGTATTTGTGCGAGGAGGG+CGG 0.633035 2_3:+55336642 Msa0300010:CDS
GTTGAAGATGGAACAGAAGG+TGG 0.634859 2_3:+55339296 Msa0300010:three_prime_UTR
GAGATGGTTGTGTACAACCA+GGG 0.638365 2_3:+55336683 Msa0300010:CDS
TTGTACACAACCATCTCAAG+AGG 0.647753 2_3:-55336677 None:intergenic
TTAATGGCAACAGGCAGTGG+CGG 0.648108 2_3:-55338398 None:intergenic
TAAATTCGCTCACGACATTG+GGG 0.660354 2_3:+55336772 Msa0300010:CDS
GAAGGAACACGATAATATGG+TGG 0.676501 2_3:+55338291 Msa0300010:three_prime_UTR
GTTAATGAAGAGAAAGCCAA+GGG 0.677516 2_3:-55337757 None:intergenic
TGTCGTGAGCGAATTTACAG+TGG 0.707236 2_3:-55336766 None:intergenic
ACTGCCTGTTGCCATTAACG+AGG 0.709980 2_3:+55338403 Msa0300010:three_prime_UTR
CCACCCACGCTACAAAACTG+AGG 0.721664 2_3:+55336814 Msa0300010:CDS
AGATGGTTGTGTACAACCAG+GGG 0.741581 2_3:+55336684 Msa0300010:CDS
GAATGACTATGGTTGGACTG+TGG 0.741878 2_3:+55339469 Msa0300010:three_prime_UTR
ATATGCAAGAAAGTGTACGA+TGG 0.782498 2_3:-55339046 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTTTGAATAACAAA+GGG - chr2_3:55337417-55337436 None:intergenic 10.0%
!!! AATTATATTTTGAATAACAA+AGG - chr2_3:55337418-55337437 None:intergenic 10.0%
!! TTATGCTGTATTAATTAATA+AGG + chr2_3:55337268-55337287 Msa0300010:intron 15.0%
!!! TTATATTTTGAATAACAAAG+GGG - chr2_3:55337416-55337435 None:intergenic 15.0%
!!! TATGCAAATCATTTTAAAAA+AGG - chr2_3:55338873-55338892 None:intergenic 15.0%
!!! TGTATATGATATTTTTTCAA+AGG - chr2_3:55338937-55338956 None:intergenic 15.0%
!!! CTAATTTAGAATTTTTGTCT+TGG - chr2_3:55337059-55337078 None:intergenic 20.0%
!! TAATAAGGTAACATGTTTAT+AGG + chr2_3:55337283-55337302 Msa0300010:intron 20.0%
!!! AGTGGTAATATATTTTTGTT+AGG + chr2_3:55337532-55337551 Msa0300010:intron 20.0%
!!! GATTGAATGCTATTTATTTT+TGG + chr2_3:55337625-55337644 Msa0300010:intron 20.0%
!! CAGATTATAATCTATTAAGT+TGG + chr2_3:55337652-55337671 Msa0300010:intron 20.0%
!! TTCTTTCTTGTCTAATTATA+TGG + chr2_3:55338024-55338043 Msa0300010:intron 20.0%
!! AAAGTTTCTATAGATAAAGA+AGG - chr2_3:55338173-55338192 None:intergenic 20.0%
!! TAGACACAAAAAAAAACAAA+TGG - chr2_3:55338529-55338548 None:intergenic 20.0%
!! AATTAAATTGAAACATAGTG+TGG - chr2_3:55338608-55338627 None:intergenic 20.0%
!!! ATTGATTTACCTAATTAGTT+TGG + chr2_3:55338625-55338644 Msa0300010:intron 20.0%
!!! AATTAAAGCATTTTTTCGAA+TGG + chr2_3:55339097-55339116 Msa0300010:three_prime_UTR 20.0%
!!! TTGAAATCTTATTTTGTAGA+AGG + chr2_3:55339255-55339274 Msa0300010:three_prime_UTR 20.0%
!! AAAAAAAAACTTCAACATCA+TGG - chr2_3:55339391-55339410 None:intergenic 20.0%
!! AAGTTGTTAATGATTTCATT+AGG + chr2_3:55339439-55339458 Msa0300010:three_prime_UTR 20.0%
!!! AAAAATTTTCCATCAAACAA+TGG - chr2_3:55339579-55339598 None:intergenic 20.0%
!!! TAAGTCACCAAATATATTTT+CGG + chr2_3:55339667-55339686 Msa0300010:three_prime_UTR 20.0%
!! AAGAAAAACTCTGAAAAGTT+TGG - chr2_3:55336998-55337017 None:intergenic 25.0%
!! GAGTTTTTCTTTTTGCAATA+TGG + chr2_3:55337007-55337026 Msa0300010:intron 25.0%
! ACATTATACAATACTTGACA+GGG - chr2_3:55337174-55337193 None:intergenic 25.0%
!!! GTAGTAACTTATTTTAGAAG+TGG + chr2_3:55337194-55337213 Msa0300010:intron 25.0%
! TAATACAGCATAAAATGAGA+AGG - chr2_3:55337261-55337280 None:intergenic 25.0%
! TTCCTCATTCTATATATCTT+TGG + chr2_3:55337369-55337388 Msa0300010:intron 25.0%
! AACCAAAGATATATAGAATG+AGG - chr2_3:55337374-55337393 None:intergenic 25.0%
!! AAGGGGAAAGTATATTTAAA+AGG - chr2_3:55337399-55337418 None:intergenic 25.0%
! TCACTTATCTTATTCTTTCT+GGG + chr2_3:55337682-55337701 Msa0300010:intron 25.0%
! AGAAATAAAAATAGAGAGCA+TGG - chr2_3:55337963-55337982 None:intergenic 25.0%
! AAGAAAGAATCATACAAACA+TGG - chr2_3:55338013-55338032 None:intergenic 25.0%
!!! GATTAATTTTTGTTTGCAGA+TGG + chr2_3:55338057-55338076 Msa0300010:intron 25.0%
!! CATTTTGCCTAATAGAATTT+AGG + chr2_3:55338439-55338458 Msa0300010:three_prime_UTR 25.0%
! GAACTTACCTAAATTCTATT+AGG - chr2_3:55338449-55338468 None:intergenic 25.0%
! ATAGAATTTAGGTAAGTTCT+TGG + chr2_3:55338450-55338469 Msa0300010:intron 25.0%
! TTCAAACTTCCAAACTAATT+AGG - chr2_3:55338637-55338656 None:intergenic 25.0%
! CTAATTTCAACTTATTAGGA+AGG - chr2_3:55338687-55338706 None:intergenic 25.0%
! CTCACTAATTTCAACTTATT+AGG - chr2_3:55338691-55338710 None:intergenic 25.0%
!! CTAATAAGTTGAAATTAGTG+AGG + chr2_3:55338689-55338708 Msa0300010:intron 25.0%
!!! ATTAGTGAGGTTTTTCAATT+TGG + chr2_3:55338702-55338721 Msa0300010:intron 25.0%
! ATAGTATGAGCTAATAATCT+CGG + chr2_3:55338762-55338781 Msa0300010:intron 25.0%
!! TGCATATTCATTATGCATTT+TGG + chr2_3:55338887-55338906 Msa0300010:intron 25.0%
! ATGACATGATACTTATAGAT+TGG + chr2_3:55339321-55339340 Msa0300010:three_prime_UTR 25.0%
! TGACATGATACTTATAGATT+GGG + chr2_3:55339322-55339341 Msa0300010:three_prime_UTR 25.0%
! TTATAGATTGGGTTCTATAT+TGG + chr2_3:55339333-55339352 Msa0300010:three_prime_UTR 25.0%
! TATAGATTGGGTTCTATATT+GGG + chr2_3:55339334-55339353 Msa0300010:three_prime_UTR 25.0%
! ACAAAATAAAGAGCAAGAAT+TGG - chr2_3:55339606-55339625 None:intergenic 25.0%
!! AAGTCACCAAATATATTTTC+GGG + chr2_3:55339668-55339687 Msa0300010:three_prime_UTR 25.0%
! GAAAAACCCGAAAATATATT+TGG - chr2_3:55339677-55339696 None:intergenic 25.0%
!!! ATATATTTTCGGGTTTTTCA+TGG + chr2_3:55339678-55339697 Msa0300010:three_prime_UTR 25.0%
! TTAGCTTACAATTAACTGAT+AGG - chr2_3:55339724-55339743 None:intergenic 25.0%
TGTTCATTTACAGTATAGCA+TGG + chr2_3:55337100-55337119 Msa0300010:intron 30.0%
CACATTATACAATACTTGAC+AGG - chr2_3:55337175-55337194 None:intergenic 30.0%
AGGTATCATGTTCATAGTAT+TGG + chr2_3:55337507-55337526 Msa0300010:intron 30.0%
!! ATGTTCATAGTATTGGTTAG+TGG + chr2_3:55337514-55337533 Msa0300010:intron 30.0%
! TTTTTGTTAGGCACACTAAA+AGG + chr2_3:55337544-55337563 Msa0300010:intron 30.0%
CTCACTTATCTTATTCTTTC+TGG + chr2_3:55337681-55337700 Msa0300010:intron 30.0%
! TTATCTTATTCTTTCTGGGA+AGG + chr2_3:55337686-55337705 Msa0300010:intron 30.0%
! TATCTTATTCTTTCTGGGAA+GGG + chr2_3:55337687-55337706 Msa0300010:intron 30.0%
TAGAGCTGAAAAAAGAGAAT+GGG - chr2_3:55337847-55337866 None:intergenic 30.0%
ATAGAGCTGAAAAAAGAGAA+TGG - chr2_3:55337848-55337867 None:intergenic 30.0%
!! AGCTCTATTTCTATCTGAAA+AGG + chr2_3:55337860-55337879 Msa0300010:intron 30.0%
ATGAAGCCAGGAAAATTAAA+AGG - chr2_3:55337888-55337907 None:intergenic 30.0%
ATACACATTATAATGAAGCC+AGG - chr2_3:55337900-55337919 None:intergenic 30.0%
GGCTTCATTATAATGTGTAT+TGG + chr2_3:55337900-55337919 Msa0300010:CDS 30.0%
TAATGTGTATTGGAATTGCT+TGG + chr2_3:55337910-55337929 Msa0300010:CDS 30.0%
AATGTGTATTGGAATTGCTT+GGG + chr2_3:55337911-55337930 Msa0300010:CDS 30.0%
TATGTCCATCTAACTCAAAA+TGG - chr2_3:55338122-55338141 None:intergenic 30.0%
CTTTAATTGTGCTTCTGATA+AGG + chr2_3:55338195-55338214 Msa0300010:three_prime_UTR 30.0%
CTTCATACTAAGAAAGAAGA+AGG + chr2_3:55338273-55338292 Msa0300010:three_prime_UTR 30.0%
AAATTGAAACATAGTGTGGT+TGG - chr2_3:55338604-55338623 None:intergenic 30.0%
ACTTATTAGGAAGGATAAAG+AGG - chr2_3:55338678-55338697 None:intergenic 30.0%
ATCTCATTCAGCCAAAAATA+TGG - chr2_3:55338744-55338763 None:intergenic 30.0%
CATTAACAACTTCAACATCA+TGG - chr2_3:55339431-55339450 None:intergenic 30.0%
! AATTTTCCATCAAACAATGG+TGG - chr2_3:55339576-55339595 None:intergenic 30.0%
ATTTACAGTGGTCTCCATAA+GGG - chr2_3:55336757-55336776 None:intergenic 35.0%
AATTTACAGTGGTCTCCATA+AGG - chr2_3:55336758-55336777 None:intergenic 35.0%
! AAACTCTGAAAAGTTTGGCA+GGG - chr2_3:55336993-55337012 None:intergenic 35.0%
! AAAACTCTGAAAAGTTTGGC+AGG - chr2_3:55336994-55337013 None:intergenic 35.0%
CACTAAAAGGAAGACATGAT+TGG + chr2_3:55337557-55337576 Msa0300010:intron 35.0%
GTTAATGAAGAGAAAGCCAA+GGG - chr2_3:55337760-55337779 None:intergenic 35.0%
! CTTGGCTTTCTCTTCATTAA+CGG + chr2_3:55337759-55337778 Msa0300010:CDS 35.0%
! TTGGCTTTCTCTTCATTAAC+GGG + chr2_3:55337760-55337779 Msa0300010:CDS 35.0%
CTGTAGTAATGTGAACATGA+TGG - chr2_3:55337807-55337826 None:intergenic 35.0%
!!! AAGGCACCTTTTAATTTTCC+TGG + chr2_3:55337879-55337898 Msa0300010:CDS 35.0%
!!! TCCTTCCATTTTGAGTTAGA+TGG + chr2_3:55338114-55338133 Msa0300010:exon 35.0%
TCCATCTAACTCAAAATGGA+AGG - chr2_3:55338118-55338137 None:intergenic 35.0%
GAAGAAGGAACACGATAATA+TGG + chr2_3:55338288-55338307 Msa0300010:three_prime_UTR 35.0%
TCTTGAAAGATCCTCGTTAA+TGG - chr2_3:55338417-55338436 None:intergenic 35.0%
! TTCAAGTGCAGCCATATTTT+TGG + chr2_3:55338730-55338749 Msa0300010:intron 35.0%
!! AGCAGTATTTTTGATCTAGC+AGG + chr2_3:55338985-55339004 Msa0300010:intron 35.0%
TGTACGATGGTGTTTAAACT+TGG - chr2_3:55339036-55339055 None:intergenic 35.0%
! ATATGCAAGAAAGTGTACGA+TGG - chr2_3:55339049-55339068 None:intergenic 35.0%
AGAACAAGCACAAATGTTAG+AGG + chr2_3:55339146-55339165 Msa0300010:three_prime_UTR 35.0%
GAACAAGCACAAATGTTAGA+GGG + chr2_3:55339147-55339166 Msa0300010:three_prime_UTR 35.0%
ACAAATGTTAGAGGGACAAT+GGG + chr2_3:55339155-55339174 Msa0300010:three_prime_UTR 35.0%
!! ATATCCACTTTTGCAGTGAT+AGG - chr2_3:55339212-55339231 None:intergenic 35.0%
AAAGTGGATATCTACAGCTT+TGG + chr2_3:55339221-55339240 Msa0300010:three_prime_UTR 35.0%
GTTCTATATTGGGCAAAAGA+GGG + chr2_3:55339344-55339363 Msa0300010:three_prime_UTR 35.0%
TTCTATATTGGGCAAAAGAG+GGG + chr2_3:55339345-55339364 Msa0300010:three_prime_UTR 35.0%
TAGGTTTGAGAGAATGACTA+TGG + chr2_3:55339458-55339477 Msa0300010:three_prime_UTR 35.0%
TTTGAGAGAATGACTATGGT+TGG + chr2_3:55339462-55339481 Msa0300010:three_prime_UTR 35.0%
ATGGCCTAATAGTTGGATTA+GGG - chr2_3:55339503-55339522 None:intergenic 35.0%
TATTAGGCCATCTATTGCAA+AGG + chr2_3:55339512-55339531 Msa0300010:three_prime_UTR 35.0%
CTGATAGGAGAAAGAGAAAA+AGG - chr2_3:55339709-55339728 None:intergenic 35.0%
AACAGAGCAAACATACTTTG+TGG + chr2_3:55339756-55339775 Msa0300010:three_prime_UTR 35.0%
GACAACCTCTCACATATTTA+AGG - chr2_3:55339783-55339802 None:intergenic 35.0%
!! AAAAGGTATTTGTGCGAGGA+GGG + chr2_3:55336639-55336658 Msa0300010:CDS 40.0%
TTGTACACAACCATCTCAAG+AGG - chr2_3:55336680-55336699 None:intergenic 40.0%
AGACTGAGCTCTGTAACAAA+TGG + chr2_3:55336714-55336733 Msa0300010:CDS 40.0%
TGTAACAAATGGCAGGAAAC+TGG + chr2_3:55336725-55336744 Msa0300010:CDS 40.0%
TGTAAATTCGCTCACGACAT+TGG + chr2_3:55336770-55336789 Msa0300010:CDS 40.0%
GTAAATTCGCTCACGACATT+GGG + chr2_3:55336771-55336790 Msa0300010:CDS 40.0%
TAAATTCGCTCACGACATTG+GGG + chr2_3:55336772-55336791 Msa0300010:CDS 40.0%
AAATGGCATCTATGGCCATA+CGG - chr2_3:55336872-55336891 None:intergenic 40.0%
CTTGTTCAGTAAGTGCATGA+CGG - chr2_3:55336895-55336914 None:intergenic 40.0%
TAGCTAAAGACATACCCAAG+AGG - chr2_3:55337127-55337146 None:intergenic 40.0%
TGAGGAATGAAACTCACACA+CGG - chr2_3:55337356-55337375 None:intergenic 40.0%
GGAACACAGTCAACTTCTAA+TGG + chr2_3:55337578-55337597 Msa0300010:intron 40.0%
TGCACATATTGTATCTCCCT+TGG + chr2_3:55337741-55337760 Msa0300010:CDS 40.0%
CGTTAATGAAGAGAAAGCCA+AGG - chr2_3:55337761-55337780 None:intergenic 40.0%
TCAGAGTATGATGCTTAGAG+TGG + chr2_3:55338090-55338109 Msa0300010:CDS 40.0%
GTGCTTCTGATAAGGAAGAA+AGG + chr2_3:55338203-55338222 Msa0300010:three_prime_UTR 40.0%
GAAGGAACACGATAATATGG+TGG + chr2_3:55338291-55338310 Msa0300010:three_prime_UTR 40.0%
TATTCCTTCTATGAGATGCC+TGG + chr2_3:55338359-55338378 Msa0300010:three_prime_UTR 40.0%
TCTGTCTTTCATTAGCAGCT+TGG - chr2_3:55339123-55339142 None:intergenic 40.0%
CACAAATGTTAGAGGGACAA+TGG + chr2_3:55339154-55339173 Msa0300010:three_prime_UTR 40.0%
!! GAGTTGCATCAAGTTGAAGA+TGG + chr2_3:55339284-55339303 Msa0300010:three_prime_UTR 40.0%
! CAAGTTGAAGATGGAACAGA+AGG + chr2_3:55339293-55339312 Msa0300010:three_prime_UTR 40.0%
GGTTCTATATTGGGCAAAAG+AGG + chr2_3:55339343-55339362 Msa0300010:three_prime_UTR 40.0%
TGTGCCCTAATCCAACTATT+AGG + chr2_3:55339496-55339515 Msa0300010:three_prime_UTR 40.0%
GATGGCCTAATAGTTGGATT+AGG - chr2_3:55339504-55339523 None:intergenic 40.0%
GCAATAGATGGCCTAATAGT+TGG - chr2_3:55339510-55339529 None:intergenic 40.0%
TGCAACACCTTTGCAATAGA+TGG - chr2_3:55339522-55339541 None:intergenic 40.0%
AGTGTTCCACCATTGTTTGA+TGG + chr2_3:55339567-55339586 Msa0300010:three_prime_UTR 40.0%
GTGGTCCTTAAATATGTGAG+AGG + chr2_3:55339775-55339794 Msa0300010:three_prime_UTR 40.0%
!! CAGCAAAAGGTATTTGTGCG+AGG + chr2_3:55336635-55336654 Msa0300010:CDS 45.0%
!! CAAAAGGTATTTGTGCGAGG+AGG + chr2_3:55336638-55336657 Msa0300010:CDS 45.0%
TGAGATGGTTGTGTACAACC+AGG + chr2_3:55336682-55336701 Msa0300010:CDS 45.0%
GAGATGGTTGTGTACAACCA+GGG + chr2_3:55336683-55336702 Msa0300010:CDS 45.0%
AGATGGTTGTGTACAACCAG+GGG + chr2_3:55336684-55336703 Msa0300010:CDS 45.0%
TGAGCTCTGTAACAAATGGC+AGG + chr2_3:55336718-55336737 Msa0300010:CDS 45.0%
TGTCGTGAGCGAATTTACAG+TGG - chr2_3:55336769-55336788 None:intergenic 45.0%
!! AGACCTCAGTTTTGTAGCGT+GGG - chr2_3:55336820-55336839 None:intergenic 45.0%
AATGGCATCTATGGCCATAC+GGG - chr2_3:55336871-55336890 None:intergenic 45.0%
! CATGACGGAAATGGCATCTA+TGG - chr2_3:55336880-55336899 None:intergenic 45.0%
! CAGTAAGTGCATGACGGAAA+TGG - chr2_3:55336889-55336908 None:intergenic 45.0%
ACAGTATAGCATGGTCCTCT+TGG + chr2_3:55337109-55337128 Msa0300010:intron 45.0%
CAGTATAGCATGGTCCTCTT+GGG + chr2_3:55337110-55337129 Msa0300010:intron 45.0%
GAGAACATTGCAGCAGCATA+AGG - chr2_3:55337311-55337330 None:intergenic 45.0%
GAACATGATACCTGCTCACA+GGG - chr2_3:55337500-55337519 None:intergenic 45.0%
TGAACATGATACCTGCTCAC+AGG - chr2_3:55337501-55337520 None:intergenic 45.0%
! TCTCTTCATTAACGGGTGAG+TGG + chr2_3:55337767-55337786 Msa0300010:intron 45.0%
AATGCCAGGCATCTCATAGA+AGG - chr2_3:55338366-55338385 None:intergenic 45.0%
AGATCCTCGTTAATGGCAAC+AGG - chr2_3:55338410-55338429 None:intergenic 45.0%
! GATAAAGAGGTGTGACAGCA+AGG - chr2_3:55338665-55338684 None:intergenic 45.0%
TCACTGTCGAGAAAGATGCT+CGG - chr2_3:55338832-55338851 None:intergenic 45.0%
TAGAGGGACAATGGGATGTT+TGG + chr2_3:55339163-55339182 Msa0300010:three_prime_UTR 45.0%
!! GGTGCATTTTTGAGCCATTC+TGG - chr2_3:55339191-55339210 None:intergenic 45.0%
TGCACCTATCACTGCAAAAG+TGG + chr2_3:55339205-55339224 Msa0300010:three_prime_UTR 45.0%
GTTGAAGATGGAACAGAAGG+TGG + chr2_3:55339296-55339315 Msa0300010:three_prime_UTR 45.0%
GAATGACTATGGTTGGACTG+TGG + chr2_3:55339469-55339488 Msa0300010:three_prime_UTR 45.0%
!! TGCAAAGGTGTTGCAGATGT+CGG + chr2_3:55339527-55339546 Msa0300010:three_prime_UTR 45.0%
AGAGGAAGAGCCTCTTGAGA+TGG + chr2_3:55336667-55336686 Msa0300010:CDS 50.0%
GAAACTGGCACATGCCCTTA+TGG + chr2_3:55336740-55336759 Msa0300010:CDS 50.0%
!! CAGACCTCAGTTTTGTAGCG+TGG - chr2_3:55336821-55336840 None:intergenic 50.0%
GCTACAAAACTGAGGTCTGC+AGG + chr2_3:55336822-55336841 Msa0300010:CDS 50.0%
CAAAACTGAGGTCTGCAGGA+TGG + chr2_3:55336826-55336845 Msa0300010:CDS 50.0%
ACAAGATGATCCCTGTGAGC+AGG + chr2_3:55337487-55337506 Msa0300010:CDS 50.0%
! TTCTTTCTGGGAAGGGCTCA+TGG + chr2_3:55337694-55337713 Msa0300010:intron 50.0%
! GGCATTTGGCTCATAATGCC+AGG - chr2_3:55338380-55338399 None:intergenic 50.0%
TTAATGGCAACAGGCAGTGG+CGG - chr2_3:55338401-55338420 None:intergenic 50.0%
TCGTTAATGGCAACAGGCAG+TGG - chr2_3:55338404-55338423 None:intergenic 50.0%
ACTGCCTGTTGCCATTAACG+AGG + chr2_3:55338403-55338422 Msa0300010:three_prime_UTR 50.0%
TGGGATGTTTGGCACCAGAA+TGG + chr2_3:55339174-55339193 Msa0300010:three_prime_UTR 50.0%
!! AAGGTGTTGCAGATGTCGGA+AGG + chr2_3:55339531-55339550 Msa0300010:three_prime_UTR 50.0%
AGGTATTTGTGCGAGGAGGG+CGG + chr2_3:55336642-55336661 Msa0300010:CDS 55.0%
GCTCAGTCTTGAACATCCCC+TGG - chr2_3:55336703-55336722 None:intergenic 55.0%
AGCGTGGGTGGCAGATCATT+GGG - chr2_3:55336805-55336824 None:intergenic 55.0%
TAGCGTGGGTGGCAGATCAT+TGG - chr2_3:55336806-55336825 None:intergenic 55.0%
!! CCTCAGTTTTGTAGCGTGGG+TGG - chr2_3:55336817-55336836 None:intergenic 55.0%
CCACCCACGCTACAAAACTG+AGG + chr2_3:55336814-55336833 Msa0300010:CDS 55.0%
TCTGCAGGATGGTCCTTGCT+GGG + chr2_3:55336837-55336856 Msa0300010:CDS 55.0%
AGGGCTCATGGTTGCATCTC+AGG + chr2_3:55337706-55337725 Msa0300010:intron 55.0%
! CGAGAAAGATGCTCGGTGAG+TGG - chr2_3:55338825-55338844 None:intergenic 55.0%
GTCTGCAGGATGGTCCTTGC+TGG + chr2_3:55336836-55336855 Msa0300010:CDS 60.0%
CTGCAGGATGGTCCTTGCTG+GGG + chr2_3:55336838-55336857 Msa0300010:CDS 60.0%
CAACAGGCAGTGGCGGCATT+TGG - chr2_3:55338394-55338413 None:intergenic 60.0%
! GATGCTCGGTGAGTGGTGAC+AGG - chr2_3:55338818-55338837 None:intergenic 60.0%
TGTGCGAGGAGGGCGGAAAG+AGG + chr2_3:55336649-55336668 Msa0300010:CDS 65.0%
ACGGGCACACAACCCCAGCA+AGG - chr2_3:55336853-55336872 None:intergenic 65.0%
GCTGGGGTTGTGTGCCCGTA+TGG + chr2_3:55336854-55336873 Msa0300010:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55336629 55339848 55336629 ID=Msa0300010;Name=Msa0300010
chr2_3 mRNA 55336629 55339848 55336629 ID=Msa0300010-mRNA-1;Parent=Msa0300010;Name=Msa0300010-mRNA-1;_AED=0.28;_eAED=0.29;_QI=0|0.66|0.71|1|0.66|0.57|7|1130|179
chr2_3 exon 55336629 55336924 55336629 ID=Msa0300010-mRNA-1:exon:17683;Parent=Msa0300010-mRNA-1
chr2_3 exon 55337467 55337508 55337467 ID=Msa0300010-mRNA-1:exon:17684;Parent=Msa0300010-mRNA-1
chr2_3 exon 55337708 55337781 55337708 ID=Msa0300010-mRNA-1:exon:17685;Parent=Msa0300010-mRNA-1
chr2_3 exon 55337862 55337932 55337862 ID=Msa0300010-mRNA-1:exon:17686;Parent=Msa0300010-mRNA-1
chr2_3 exon 55338076 55338460 55338076 ID=Msa0300010-mRNA-1:exon:17687;Parent=Msa0300010-mRNA-1
chr2_3 exon 55339007 55339387 55339007 ID=Msa0300010-mRNA-1:exon:17688;Parent=Msa0300010-mRNA-1
chr2_3 exon 55339428 55339848 55339428 ID=Msa0300010-mRNA-1:exon:17689;Parent=Msa0300010-mRNA-1
chr2_3 CDS 55336629 55336924 55336629 ID=Msa0300010-mRNA-1:cds;Parent=Msa0300010-mRNA-1
chr2_3 CDS 55337467 55337508 55337467 ID=Msa0300010-mRNA-1:cds;Parent=Msa0300010-mRNA-1
chr2_3 CDS 55337708 55337781 55337708 ID=Msa0300010-mRNA-1:cds;Parent=Msa0300010-mRNA-1
chr2_3 CDS 55337862 55337932 55337862 ID=Msa0300010-mRNA-1:cds;Parent=Msa0300010-mRNA-1
chr2_3 CDS 55338076 55338132 55338076 ID=Msa0300010-mRNA-1:cds;Parent=Msa0300010-mRNA-1
chr2_3 three_prime_UTR 55338133 55338460 55338133 ID=Msa0300010-mRNA-1:three_prime_utr;Parent=Msa0300010-mRNA-1
chr2_3 three_prime_UTR 55339007 55339387 55339007 ID=Msa0300010-mRNA-1:three_prime_utr;Parent=Msa0300010-mRNA-1
chr2_3 three_prime_UTR 55339428 55339848 55339428 ID=Msa0300010-mRNA-1:three_prime_utr;Parent=Msa0300010-mRNA-1
Gene Sequence

>Msa0300010

ATGCTGCAGCAAAAGGTATTTGTGCGAGGAGGGCGGAAAGAGGAAGAGCCTCTTGAGATGGTTGTGTACAACCAGGGGATGTTCAAGACTGAGCTCTGTAACAAATGGCAGGAAACTGGCACATGCCCTTATGGAGACCACTGTAAATTCGCTCACGACATTGGGGAGCTTCGCCCAATGATCTGCCACCCACGCTACAAAACTGAGGTCTGCAGGATGGTCCTTGCTGGGGTTGTGTGCCCGTATGGCCATAGATGCCATTTCCGTCATGCACTTACTGAACAAGAGAAAGCTATATACGAAGTATTGTTTCTAAACAAGATGATCCCTGTGAGCAGGGCTCATGGTTGCATCTCAGGCATATGTTCGATTGCACATATTGTATCTCCCTTGGCTTTCTCTTCATTAACGGCTCTATTTCTATCTGAAAAGGCACCTTTTAATTTTCCTGGCTTCATTATAATGTGTATTGGAATTGCTTGGATGGTATCTTTTGTTCAGAGTATGATGCTTAGAGTGGTTCCTTCCATTTTGAGTTAG
Protein sequence

>Msa0300010

MLQQKVFVRGGRKEEEPLEMVVYNQGMFKTELCNKWQETGTCPYGDHCKFAHDIGELRPMICHPRYKTEVCRMVLAGVVCPYGHRCHFRHALTEQEKAIYEVLFLNKMIPVSRAHGCISGICSIAHIVSPLAFSSLTALFLSEKAPFNFPGFIIMCIGIAWMVSFVQSMMLRVVPSILS*