Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1060360 | KEH22851.1 | 92.208 | 77 | 6 | 0 | 1 | 77 | 1 | 77 | 8.24e-43 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1060360 | sp|P81748|MIFH_TRITR | 38.158 | 76 | 46 | 1 | 1 | 76 | 1 | 75 | 4.73e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1060360 | A0A072U0D2 | 92.208 | 77 | 6 | 0 | 1 | 77 | 1 | 77 | 3.94e-43 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0366560 | Msa1060360 | 0.822161 | 2.746774e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1060360 | MtrunA17_Chr7g0238011 | 92.208 | 77 | 6 | 0 | 1 | 77 | 1 | 77 | 1.20e-46 | 144 |
| Msa1060360 | MtrunA17_Chr4g0005161 | 61.039 | 77 | 30 | 0 | 1 | 77 | 1 | 77 | 2.34e-28 | 98.2 |
| Msa1060360 | MtrunA17_Chr4g0071771 | 49.351 | 77 | 39 | 0 | 1 | 77 | 1 | 77 | 9.36e-20 | 76.3 |
| Msa1060360 | MtrunA17_Chr4g0005151 | 44.156 | 77 | 43 | 0 | 1 | 77 | 1 | 77 | 1.05e-19 | 76.3 |
| Msa1060360 | MtrunA17_Chr4g0071761 | 49.351 | 77 | 39 | 0 | 1 | 77 | 1 | 77 | 7.34e-19 | 73.9 |
| Msa1060360 | MtrunA17_Chr7g0243451 | 40.260 | 77 | 46 | 0 | 1 | 77 | 1 | 77 | 1.52e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1060360 | AT5G01650.2 | 61.039 | 77 | 30 | 0 | 1 | 77 | 1 | 77 | 8.04e-29 | 100 |
| Msa1060360 | AT5G01650.3 | 61.039 | 77 | 30 | 0 | 1 | 77 | 1 | 77 | 8.04e-29 | 100 |
| Msa1060360 | AT5G01650.4 | 61.039 | 77 | 30 | 0 | 1 | 77 | 1 | 77 | 9.64e-29 | 99.8 |
| Msa1060360 | AT5G01650.1 | 61.039 | 77 | 30 | 0 | 1 | 77 | 1 | 77 | 1.55e-28 | 99.0 |
| Msa1060360 | AT5G57170.2 | 46.753 | 77 | 41 | 0 | 1 | 77 | 1 | 77 | 4.52e-18 | 72.4 |
| Msa1060360 | AT5G57170.1 | 46.753 | 77 | 41 | 0 | 1 | 77 | 1 | 77 | 5.78e-18 | 72.0 |
| Msa1060360 | AT3G51660.1 | 42.857 | 77 | 44 | 0 | 1 | 77 | 1 | 77 | 2.31e-17 | 70.5 |
Find 21 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACTTCATACATATATTT+AGG | 0.180060 | 7_2:-48341256 | None:intergenic |
| TCAACAAACGTTTGCTTTGA+TGG | 0.236580 | 7_2:+48340492 | Msa1060360:CDS |
| CACAAAGATTTGTTTAAATC+TGG | 0.269566 | 7_2:-48341138 | None:intergenic |
| GGAAAGGTACGTACAGATTT+GGG | 0.269570 | 7_2:-48340576 | None:intergenic |
| TGGAAAGGTACGTACAGATT+TGG | 0.338050 | 7_2:-48340577 | None:intergenic |
| CTCTACTATTGCAAACATCT+TGG | 0.353124 | 7_2:+48340548 | Msa1060360:CDS |
| GGATCAATACCAATATGTTT+TGG | 0.376407 | 7_2:+48341060 | Msa1060360:CDS |
| TCAGTTTCACCAAAACATAT+TGG | 0.384156 | 7_2:-48341069 | None:intergenic |
| TATGGTGAATTTGTGGCTAT+TGG | 0.409602 | 7_2:+48341105 | Msa1060360:CDS |
| CAGATTTAAACAAATCTTTG+TGG | 0.414552 | 7_2:+48341139 | Msa1060360:CDS |
| AGAGTTGAGTAGAGAGAGCA+TGG | 0.421965 | 7_2:-48340529 | None:intergenic |
| TCTACTATTGCAAACATCTT+GGG | 0.427652 | 7_2:+48340549 | Msa1060360:CDS |
| AAAGCAAACGTTTGTTGAAA+GGG | 0.442435 | 7_2:-48340487 | None:intergenic |
| TATGTGACGGTTTCATTAGA+AGG | 0.443254 | 7_2:+48341039 | Msa1060360:CDS |
| CAAAGCAAACGTTTGTTGAA+AGG | 0.458324 | 7_2:-48340488 | None:intergenic |
| ACTGAGGAGCCAGCAGCATA+TGG | 0.461454 | 7_2:+48341087 | Msa1060360:CDS |
| AGCAGCATATGGTGAATTTG+TGG | 0.510208 | 7_2:+48341098 | Msa1060360:CDS |
| ACAAATTCACCATATGCTGC+TGG | 0.542569 | 7_2:-48341096 | None:intergenic |
| TTTGTTGAAAGGGTGAGACA+AGG | 0.631716 | 7_2:-48340477 | None:intergenic |
| TATTGATTTGCAGTATGTGA+CGG | 0.638900 | 7_2:+48341026 | Msa1060360:intron |
| GAAAGGTACGTACAGATTTG+GGG | 0.660125 | 7_2:-48340575 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATTATTGATTAGTTTTA+TGG | + | chr7_2:48340639-48340658 | Msa1060360:intron | 10.0% |
| !!! | TAAATTATTGATTAGTTTTA+TGG | + | chr7_2:48340639-48340658 | Msa1060360:intron | 10.0% |
| !!! | GTTTTTATTTATCTAATCCT+TGG | + | chr7_2:48340841-48340860 | Msa1060360:intron | 20.0% |
| !! | ACTACTTCATACATATATTT+AGG | - | chr7_2:48341259-48341278 | None:intergenic | 20.0% |
| !! | AATATATGTATGAAGTAGTT+AGG | + | chr7_2:48341260-48341279 | Msa1060360:three_prime_UTR | 20.0% |
| !!! | GTTTTTATTTATCTAATCCT+TGG | + | chr7_2:48340841-48340860 | Msa1060360:intron | 20.0% |
| !! | ACTACTTCATACATATATTT+AGG | - | chr7_2:48341259-48341278 | None:intergenic | 20.0% |
| !!! | TTAGTTTTATGGTCATGTTA+TGG | + | chr7_2:48340650-48340669 | Msa1060360:intron | 25.0% |
| ! | ATAAATATTAGCAACCAAAG+AGG | + | chr7_2:48340746-48340765 | Msa1060360:intron | 25.0% |
| ! | CTTAGTATATTTAACCTCTT+TGG | - | chr7_2:48340763-48340782 | None:intergenic | 25.0% |
| !! | CACAAAGATTTGTTTAAATC+TGG | - | chr7_2:48341141-48341160 | None:intergenic | 25.0% |
| ! | CAGATTTAAACAAATCTTTG+TGG | + | chr7_2:48341139-48341158 | Msa1060360:CDS | 25.0% |
| !!! | TTAGTTTTATGGTCATGTTA+TGG | + | chr7_2:48340650-48340669 | Msa1060360:intron | 25.0% |
| ! | ATAAATATTAGCAACCAAAG+AGG | + | chr7_2:48340746-48340765 | Msa1060360:intron | 25.0% |
| ! | CTTAGTATATTTAACCTCTT+TGG | - | chr7_2:48340763-48340782 | None:intergenic | 25.0% |
| !! | CACAAAGATTTGTTTAAATC+TGG | - | chr7_2:48341141-48341160 | None:intergenic | 25.0% |
| ! | CAGATTTAAACAAATCTTTG+TGG | + | chr7_2:48341139-48341158 | Msa1060360:CDS | 25.0% |
| ! | AAAGCAAACGTTTGTTGAAA+GGG | - | chr7_2:48340490-48340509 | None:intergenic | 30.0% |
| TCTACTATTGCAAACATCTT+GGG | + | chr7_2:48340549-48340568 | Msa1060360:CDS | 30.0% | |
| ATACATTCATGCAAATAGAG+AGG | - | chr7_2:48340616-48340635 | None:intergenic | 30.0% | |
| !! | GTTATGGTCTTGTGAAAATA+TGG | + | chr7_2:48340666-48340685 | Msa1060360:intron | 30.0% |
| AAGTTCAATGACGTATCTTT+CGG | + | chr7_2:48340780-48340799 | Msa1060360:intron | 30.0% | |
| TAATAATCCTCCCTTGTATT+TGG | - | chr7_2:48340868-48340887 | None:intergenic | 30.0% | |
| !! | TATTGATTTGCAGTATGTGA+CGG | + | chr7_2:48341026-48341045 | Msa1060360:intron | 30.0% |
| ! | GGATCAATACCAATATGTTT+TGG | + | chr7_2:48341060-48341079 | Msa1060360:CDS | 30.0% |
| TCAGTTTCACCAAAACATAT+TGG | - | chr7_2:48341072-48341091 | None:intergenic | 30.0% | |
| !!! | AATATGTTTTGGTGAAACTG+AGG | + | chr7_2:48341071-48341090 | Msa1060360:CDS | 30.0% |
| ! | AAAGCAAACGTTTGTTGAAA+GGG | - | chr7_2:48340490-48340509 | None:intergenic | 30.0% |
| TCTACTATTGCAAACATCTT+GGG | + | chr7_2:48340549-48340568 | Msa1060360:CDS | 30.0% | |
| ATACATTCATGCAAATAGAG+AGG | - | chr7_2:48340616-48340635 | None:intergenic | 30.0% | |
| !! | GTTATGGTCTTGTGAAAATA+TGG | + | chr7_2:48340666-48340685 | Msa1060360:intron | 30.0% |
| AAGTTCAATGACGTATCTTT+CGG | + | chr7_2:48340780-48340799 | Msa1060360:intron | 30.0% | |
| TAATAATCCTCCCTTGTATT+TGG | - | chr7_2:48340868-48340887 | None:intergenic | 30.0% | |
| !! | TATTGATTTGCAGTATGTGA+CGG | + | chr7_2:48341026-48341045 | Msa1060360:intron | 30.0% |
| ! | GGATCAATACCAATATGTTT+TGG | + | chr7_2:48341060-48341079 | Msa1060360:CDS | 30.0% |
| TCAGTTTCACCAAAACATAT+TGG | - | chr7_2:48341072-48341091 | None:intergenic | 30.0% | |
| !!! | AATATGTTTTGGTGAAACTG+AGG | + | chr7_2:48341071-48341090 | Msa1060360:CDS | 30.0% |
| ! | CAAAGCAAACGTTTGTTGAA+AGG | - | chr7_2:48340491-48340510 | None:intergenic | 35.0% |
| TCAACAAACGTTTGCTTTGA+TGG | + | chr7_2:48340492-48340511 | Msa1060360:CDS | 35.0% | |
| CTCTACTATTGCAAACATCT+TGG | + | chr7_2:48340548-48340567 | Msa1060360:CDS | 35.0% | |
| TAATCCTTGGTCCAAATACA+AGG | + | chr7_2:48340854-48340873 | Msa1060360:intron | 35.0% | |
| AATCCTTGGTCCAAATACAA+GGG | + | chr7_2:48340855-48340874 | Msa1060360:intron | 35.0% | |
| TATGTGACGGTTTCATTAGA+AGG | + | chr7_2:48341039-48341058 | Msa1060360:CDS | 35.0% | |
| TATGGTGAATTTGTGGCTAT+TGG | + | chr7_2:48341105-48341124 | Msa1060360:CDS | 35.0% | |
| ! | CAAAGCAAACGTTTGTTGAA+AGG | - | chr7_2:48340491-48340510 | None:intergenic | 35.0% |
| TCAACAAACGTTTGCTTTGA+TGG | + | chr7_2:48340492-48340511 | Msa1060360:CDS | 35.0% | |
| CTCTACTATTGCAAACATCT+TGG | + | chr7_2:48340548-48340567 | Msa1060360:CDS | 35.0% | |
| TAATCCTTGGTCCAAATACA+AGG | + | chr7_2:48340854-48340873 | Msa1060360:intron | 35.0% | |
| AATCCTTGGTCCAAATACAA+GGG | + | chr7_2:48340855-48340874 | Msa1060360:intron | 35.0% | |
| TATGTGACGGTTTCATTAGA+AGG | + | chr7_2:48341039-48341058 | Msa1060360:CDS | 35.0% | |
| TATGGTGAATTTGTGGCTAT+TGG | + | chr7_2:48341105-48341124 | Msa1060360:CDS | 35.0% | |
| TTTGTTGAAAGGGTGAGACA+AGG | - | chr7_2:48340480-48340499 | None:intergenic | 40.0% | |
| ! | GAAAGGTACGTACAGATTTG+GGG | - | chr7_2:48340578-48340597 | None:intergenic | 40.0% |
| ! | GGAAAGGTACGTACAGATTT+GGG | - | chr7_2:48340579-48340598 | None:intergenic | 40.0% |
| ! | TGGAAAGGTACGTACAGATT+TGG | - | chr7_2:48340580-48340599 | None:intergenic | 40.0% |
| GGCTTAAGAAAGAGATGGAA+AGG | - | chr7_2:48340595-48340614 | None:intergenic | 40.0% | |
| AGAGAGGCTTAAGAAAGAGA+TGG | - | chr7_2:48340600-48340619 | None:intergenic | 40.0% | |
| ACAAATTCACCATATGCTGC+TGG | - | chr7_2:48341099-48341118 | None:intergenic | 40.0% | |
| AGCAGCATATGGTGAATTTG+TGG | + | chr7_2:48341098-48341117 | Msa1060360:CDS | 40.0% | |
| TTTGTTGAAAGGGTGAGACA+AGG | - | chr7_2:48340480-48340499 | None:intergenic | 40.0% | |
| ! | GAAAGGTACGTACAGATTTG+GGG | - | chr7_2:48340578-48340597 | None:intergenic | 40.0% |
| ! | GGAAAGGTACGTACAGATTT+GGG | - | chr7_2:48340579-48340598 | None:intergenic | 40.0% |
| ! | TGGAAAGGTACGTACAGATT+TGG | - | chr7_2:48340580-48340599 | None:intergenic | 40.0% |
| GGCTTAAGAAAGAGATGGAA+AGG | - | chr7_2:48340595-48340614 | None:intergenic | 40.0% | |
| AGAGAGGCTTAAGAAAGAGA+TGG | - | chr7_2:48340600-48340619 | None:intergenic | 40.0% | |
| ACAAATTCACCATATGCTGC+TGG | - | chr7_2:48341099-48341118 | None:intergenic | 40.0% | |
| AGCAGCATATGGTGAATTTG+TGG | + | chr7_2:48341098-48341117 | Msa1060360:CDS | 40.0% | |
| AGAGTTGAGTAGAGAGAGCA+TGG | - | chr7_2:48340532-48340551 | None:intergenic | 45.0% | |
| AGAGTTGAGTAGAGAGAGCA+TGG | - | chr7_2:48340532-48340551 | None:intergenic | 45.0% | |
| CCTCCCTTGTATTTGGACCA+AGG | - | chr7_2:48340861-48340880 | None:intergenic | 50.0% | |
| CCTTGGTCCAAATACAAGGG+AGG | + | chr7_2:48340858-48340877 | Msa1060360:intron | 50.0% | |
| CCTCCCTTGTATTTGGACCA+AGG | - | chr7_2:48340861-48340880 | None:intergenic | 50.0% | |
| CCTTGGTCCAAATACAAGGG+AGG | + | chr7_2:48340858-48340877 | Msa1060360:intron | 50.0% | |
| ACTGAGGAGCCAGCAGCATA+TGG | + | chr7_2:48341087-48341106 | Msa1060360:CDS | 55.0% | |
| ACTGAGGAGCCAGCAGCATA+TGG | + | chr7_2:48341087-48341106 | Msa1060360:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 48340469 | 48341280 | 48340469 | ID=Msa1060360;Name=Msa1060360 |
| chr7_2 | mRNA | 48340469 | 48341280 | 48340469 | ID=Msa1060360-mRNA-1;Parent=Msa1060360;Name=Msa1060360-mRNA-1;_AED=0.02;_eAED=0.02;_QI=5|1|1|1|1|1|2|113|79 |
| chr7_2 | exon | 48340469 | 48340584 | 48340469 | ID=Msa1060360-mRNA-1:exon:8730;Parent=Msa1060360-mRNA-1 |
| chr7_2 | exon | 48341039 | 48341280 | 48341039 | ID=Msa1060360-mRNA-1:exon:8731;Parent=Msa1060360-mRNA-1 |
| chr7_2 | five_prime_UTR | 48340469 | 48340473 | 48340469 | ID=Msa1060360-mRNA-1:five_prime_utr;Parent=Msa1060360-mRNA-1 |
| chr7_2 | CDS | 48340474 | 48340584 | 48340474 | ID=Msa1060360-mRNA-1:cds;Parent=Msa1060360-mRNA-1 |
| chr7_2 | CDS | 48341039 | 48341167 | 48341039 | ID=Msa1060360-mRNA-1:cds;Parent=Msa1060360-mRNA-1 |
| chr7_2 | three_prime_UTR | 48341168 | 48341280 | 48341168 | ID=Msa1060360-mRNA-1:three_prime_utr;Parent=Msa1060360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
