AlfalfaGEDB | Msa1063120

M. sativa cultivar zhongmu-4 / Msa1063120

KEGG: -
GO: -
PFAM: PF03140.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063120	XP_003623251.1	54.802	177	73	3	5	174	39	215	8.06e-50	194
Msa1063120	XP_003623251.1	70.755	530	142	5	181	708	31	549	0.0	755

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063120	sp\|Q9SD53\|Y3720_ARATH	23.969	509	278	25	211	704	44	458	3.16e-12	73.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063120	G7KRC7	54.802	177	73	3	5	174	39	215	3.85e-50	194
Msa1063120	G7KRC7	70.755	530	142	5	181	708	31	549	0.0	755

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0632370	Msa1063120	0.802745	4.849353e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063120	MtrunA17_Chr7g0242011	70.755	530	142	5	181	708	31	549	0.0	755
Msa1063120	MtrunA17_Chr7g0242011	54.802	177	73	3	5	174	39	215	7.42e-54	194
Msa1063120	MtrunA17_Chr7g0242181	63.128	537	174	9	187	708	10	537	0.0	643
Msa1063120	MtrunA17_Chr7g0242181	60.000	205	75	2	8	205	15	219	1.94e-68	234
Msa1063120	MtrunA17_Chr7g0242101	54.366	607	177	12	191	708	2	597	0.0	578
Msa1063120	MtrunA17_Chr7g0242101	56.571	175	55	3	10	163	5	179	4.23e-50	184
Msa1063120	MtrunA17_Chr7g0242101	47.761	67	35	0	139	205	229	295	6.72e-11	65.5
Msa1063120	MtrunA17_Chr7g0242151	52.803	553	208	8	193	708	9	545	0.0	542
Msa1063120	MtrunA17_Chr7g0242151	73.034	178	44	1	1	174	1	178	2.46e-79	263
Msa1063120	MtrunA17_Chr7g0242091	52.793	555	210	13	187	708	28	563	0.0	528

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063120	AT4G31980.1	30.204	245	139	7	31	253	295	529	2.51e-19	93.2
Msa1063120	AT4G31980.1	28.351	194	120	8	185	373	263	442	2.97e-12	70.5
Msa1063120	AT5G11290.2	35.897	156	91	4	35	187	39	188	5.63e-17	84.3
Msa1063120	AT5G11290.2	32.727	165	98	6	212	375	32	184	2.04e-12	70.1
Msa1063120	AT3G50160.1	22.846	534	297	22	184	707	71	499	1.91e-15	80.1
Msa1063120	AT3G50160.1	28.221	163	112	3	31	191	106	265	1.35e-14	77.4
Msa1063120	AT3G50170.1	30.114	176	114	4	5	174	85	257	2.43e-15	80.1
Msa1063120	AT3G50170.1	30.102	196	119	8	183	371	79	263	5.03e-12	69.3
Msa1063120	AT3G50170.2	30.114	176	114	4	5	174	85	257	4.12e-15	78.6
Msa1063120	AT3G50170.2	30.102	196	119	8	183	371	79	263	4.69e-12	69.3

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Find 133 sgRNAs with CRISPR-Local

Find 484 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGAGAGCAATTATT+TGG	0.139869	7_2:-54619048	None:intergenic
AAACAGTCTGCACAAGAGTG+AGG	0.661263	7_2:-54619001	None:intergenic
AAAGTATTTGTGACAATTTG+TGG	0.414090	7_2:-54618023	None:intergenic
AAATTGGAGAGCAATTATTT+GGG	0.465642	7_2:-54619047	None:intergenic
AACATTTCAGTGGTTCCAGA+AGG	0.592202	7_2:+54612038	Msa1063120:CDS
AACTAAAGCGCTGTGCATCG+AGG	0.688027	7_2:+54618289	Msa1063120:CDS
AAGATCAACTCCAAACGACA+TGG	0.610920	7_2:-54618805	None:intergenic
AAGCAGCCGAAGTAAATTTC+CGG	0.336936	7_2:-54618668	None:intergenic
AATATTTCTGCTGCTGAGTC+CGG	0.384543	7_2:+54618606	Msa1063120:CDS
AATCTTATTCTGTCTATCAT+AGG	0.412582	7_2:+54618111	Msa1063120:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAATCAAACAACATTTCAG+TGG	+	chr7_2:54612028-54612047	Msa1063120:CDS	30.0%
TTCCAGAAGGACTTAAAAAA+TGG	+	chr7_2:54612051-54612070	Msa1063120:CDS	30.0%
CTCTTTATCTAATTCCCAAA+CGG	-	chr7_2:54612228-54612247	None:intergenic	30.0%
ACTCGCTAAAATTATGTTAG+TGG	+	chr7_2:54612292-54612311	Msa1063120:CDS	30.0%
GCTAAAATTATGTTAGTGGA+TGG	+	chr7_2:54612296-54612315	Msa1063120:CDS	30.0%
ACTTATGAAGAACCAAAAAG+GGG	-	chr7_2:54612460-54612479	None:intergenic	30.0%
CAACTTATGAAGAACCAAAA+AGG	-	chr7_2:54612462-54612481	None:intergenic	30.0%
CACATATGGAAAATGTGTTT+GGG	-	chr7_2:54612486-54612505	None:intergenic	30.0%
ACACATATGGAAAATGTGTT+TGG	-	chr7_2:54612487-54612506	None:intergenic	30.0%
TAAACACAAATTCCCATGTT+GGG	+	chr7_2:54612563-54612582	Msa1063120:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	54611951	54619055	54611951	ID=Msa1063120;Name=Msa1063120
chr7_2	mRNA	54611951	54619055	54611951	ID=Msa1063120-mRNA-1;Parent=Msa1063120;Name=Msa1063120-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|0\|0\|1\|1\|1\|2\|0\|708
chr7_2	exon	54611951	54612466	54611951	ID=Msa1063120-mRNA-1:exon:10038;Parent=Msa1063120-mRNA-1
chr7_2	exon	54617445	54619055	54617445	ID=Msa1063120-mRNA-1:exon:10039;Parent=Msa1063120-mRNA-1
chr7_2	CDS	54611951	54612466	54611951	ID=Msa1063120-mRNA-1:cds;Parent=Msa1063120-mRNA-1
chr7_2	CDS	54617445	54619055	54617445	ID=Msa1063120-mRNA-1:cds;Parent=Msa1063120-mRNA-1

Gene Sequence

>Msa1063120

ATGGCCAATCTAACAACGAAAAGTGAGTGGTTACAATCTATAGAAACATCGCTAACATCTGAAGACTATGATTATGTTCAATCAAACAACATTTCAGTGGTTCCAGAAGGACTTAAAAAATGGAACGAGGATGCTTACATTCCAAGGAAGGTGTCGATCGGACCAAGACTTAGGGGAAGGAGAGAAGACCTTTTGCTTATGGAAGAGGTCAAACTACGATGCATGTTGTCTCTTCTTAACCGAGCAGATAAAAGCTTGGAGCAATGCAGTGATGCCGTTTGGGAATTAGATAAAGAGGTTCGTGAATGCTATCTAGGAGACCTTAAACTCGAACGACATGAACTCGCTAAAATTATGTTAGTGGATGGATGTTTTCTGTTGGAGCTTCTCATATCAAAGGGATTAGATTCTCAACTTCCAAGCCGTTTATTTCCGCCTAGTCCCGCAACTGAAGTGCTGAAAGACGATGATGTTCTCTCTGATCTAATGTTGTTGGAGAACCAGATCCCCTTTTTGACCATGGCCAATTATCAAGTACTGAGCTATGACGAAGCAAGGGATGAAACCAACATGAATGAATGGTTAAATTCTATAGACATGTTGCTGACATCTGTGGACTTCAACTATATTCAATCATGTAGCATTTCCATCGTTCCAGAAGAGCTTATAAACTCATCAAACAAAGAAGATTACTTGCCAAAGGTGGTTTCAATTGGACCCAGATTCAAGGGAAGCAAGGAAGACTTAAATCTCATGGAAGAAGTCAAATTACGCTCGATGATGTACCTGTTACATCGAGCAGGAGAGCCGAAGGATTCCCCAAAATACTTTCGGGAATGTGGTGAAGCCATATGGAAATCAGATGAACAAGTTCGAGCTAGTTATGTGTCTGACATTAAACTAGAAAAGAAAGAACTGGCAAAAATAATGTTAATGGATGGATGTTTTCTGTTGGAGCTTGTGATTACAAAAGGGTTCAATTCTAAACTTCCAAGCCGGTTGCGCTCACCCAGTCCCGCATCTAAAGTGCTGAAAGGCGATGATGTTCTGTCTGATCTTATGTTGTTGGAGAATCAAATACCAATTCTAGTACTCCACAAATTGTCACAAATACTTTTCCCTGATGAGTTTCCTGAACCAGAGTATCTGGAGGACATGAAGGACGGAGCGGAAAAGATTAATAATCTTATTCTGTCTATCATAGGCTACGCTCAGCCTGAAGTTCAAAATCCAAGCTACTTCAAAGCTCCTCATATTCTTGATATTGTCCACTTTTTCGTTAACAGAAAAGGTGAGACAAAAAGTGATGGGGTTGTTAATTATGTAGTGCCCATTATTGGTACTCAGCATCCGAAGCCCAAACTAAAGCGCTGTGCATCGAGGCTCCAAGCTGCGGGGGTGACTATCAAACTTGCTGGAGAAAAACACAGTGGCAATTCTTGCTTTAGTTTCCTTCGGAACTGTTTTGGTGGTATATGCATAAGGCTTGGCAATATGCTTGTCACAACTAATACTCAAGTGGATGCGTCGGAAGAAGTTAAAGGTTTGAACTTTTACTTCAACTTCGAAAAGGGGAAATTTGAAATTGCACGGTTAGAGATCACCAAAACAACGAAAGCAAAGTGGCGCAATATCATTGCTTGGGAGCATCATAAAAACAACTGGAAATGTTTTGAAAATATTTCTGCTGCTGAGTCCGGGAGCAATAACAATAACCAGGTCAATACGAGTAGTCTCAGCCGGAAATTTACTTCGGCTGCTTTGATTTTCGATGGATTGGTTTGTTGTGCGGCTGATGTGAAGCTTCTTAAGGACAAGAATATCATTGTGGATCATTTGAAGATGAGTAACCAGGAGTTGGAAGAGTTTTTTCATTCCATGTCGTTTGGAGTTGATCTTGGGATAGTTGATTCCAGTTATGTTAAAATTGTTGATGACTTGAACAATTACTCGCGCGCATTTTTTATTCTGCGGGTCTTGAAAATATTTTCCCATCTTTTCAAGGGTCGTCTAGAATGGCTTTTCAAGTTTTTGAAAAAAAACTACAACTTTGTTGCTGCGATGCTGGCTTTCCTCACTCTTGTGCAGACTGTTTATACAGTCATATCCTACCATTTACCCAAATAA

Protein sequence

>Msa1063120

MANLTTKSEWLQSIETSLTSEDYDYVQSNNISVVPEGLKKWNEDAYIPRKVSIGPRLRGRREDLLLMEEVKLRCMLSLLNRADKSLEQCSDAVWELDKEVRECYLGDLKLERHELAKIMLVDGCFLLELLISKGLDSQLPSRLFPPSPATEVLKDDDVLSDLMLLENQIPFLTMANYQVLSYDEARDETNMNEWLNSIDMLLTSVDFNYIQSCSISIVPEELINSSNKEDYLPKVVSIGPRFKGSKEDLNLMEEVKLRSMMYLLHRAGEPKDSPKYFRECGEAIWKSDEQVRASYVSDIKLEKKELAKIMLMDGCFLLELVITKGFNSKLPSRLRSPSPASKVLKGDDVLSDLMLLENQIPILVLHKLSQILFPDEFPEPEYLEDMKDGAEKINNLILSIIGYAQPEVQNPSYFKAPHILDIVHFFVNRKGETKSDGVVNYVVPIIGTQHPKPKLKRCASRLQAAGVTIKLAGEKHSGNSCFSFLRNCFGGICIRLGNMLVTTNTQVDASEEVKGLNFYFNFEKGKFEIARLEITKTTKAKWRNIIAWEHHKNNWKCFENISAAESGSNNNNQVNTSSLSRKFTSAALIFDGLVCCAADVKLLKDKNIIVDHLKMSNQELEEFFHSMSFGVDLGIVDSSYVKIVDDLNNYSRAFFILRVLKIFSHLFKGRLEWLFKFLKKNYNFVAAMLAFLTLVQTVYTVISYHLPK*