AlfalfaGEDB | Msa1063980

M. sativa cultivar zhongmu-4 / Msa1063980

KEGG: K06620|K12590
GO: GO:0000902 GO:0000904 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0008284 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010033 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032989 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045787 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051302 GO:0051445 GO:0051446 GO:0051726 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090626 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000241 GO:2000243 GO:2001141
PFAM: PF02319.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063980	XP_003606095.2	73.377	308	19	5	1	245	108	415	3.17e-145	424

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063980	sp\|Q9FV71\|E2FB_ARATH	49.533	321	82	8	2	244	101	419	4.50e-82	256

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063980	A0A396I6V2	73.377	308	19	5	1	245	108	415	1.51e-145	424

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TFs/TRs:

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Gene ID	Type	Classification
Msa1063980	TF	E2F-DP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1024070	Msa1063980	0.885590	7.130098e-72	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063980	MtrunA17_Chr4g0025881	73.377	308	19	5	1	245	108	415	2.91e-149	424
Msa1063980	MtrunA17_Chr1g0177351	57.246	276	74	6	14	245	145	420	2.37e-94	284
Msa1063980	MtrunA17_Chr3g0142581	37.589	282	109	5	14	245	105	369	6.47e-52	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1063980	AT5G22220.3	48.750	320	86	6	2	244	101	419	4.31e-83	256
Msa1063980	AT5G22220.2	49.533	321	82	8	2	244	101	419	4.58e-83	256
Msa1063980	AT2G36010.1	50.000	282	89	6	14	245	170	449	9.26e-79	245
Msa1063980	AT2G36010.3	49.648	284	89	6	14	245	170	451	7.52e-78	243
Msa1063980	AT2G36010.2	46.071	280	80	7	18	245	220	480	1.19e-63	207
Msa1063980	AT1G47870.2	50.000	182	61	4	14	167	157	336	7.30e-49	165
Msa1063980	AT1G47870.1	50.000	182	61	4	14	167	158	337	7.46e-49	165

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Find 67 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACTTGCCCTGCTTCC+AGG	0.295888	7_2:-56280985	Msa1063980:intron
AAAGCATACGGTACCTAACA+AGG	0.595411	7_2:+56280643	None:intergenic
AACAATCACGACTTCGTGAG+TGG	0.509503	7_2:-56280312	Msa1063980:CDS
AACAATTGAGGAACGCCGGT+TGG	0.534246	7_2:-56281301	Msa1063980:CDS
AATTGAAAATTTAACAATTG+AGG	0.437163	7_2:-56281313	Msa1063980:CDS
ACAATCACGACTTCGTGAGT+GGG	0.632953	7_2:-56280311	Msa1063980:CDS
ACCCAGTCCAGAAGTTGACA+AGG	0.647730	7_2:-56280421	Msa1063980:CDS
ACCTTATTTGTTCATCCAAC+CGG	0.394452	7_2:+56281286	None:intergenic
ACTAATGTTCTTGAAGGGAT+TGG	0.429531	7_2:-56281775	Msa1063980:CDS
ACTATCCTGTATCTTCTCTG+GGG	0.718625	7_2:+56280699	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTAGTTGTCAACCAAAATAT+TGG	+	chr7_2:56280402-56280421	None:intergenic	30.0%
TAGTGTCATGAAAAAAAGGT+TGG	+	chr7_2:56280672-56280691	None:intergenic	30.0%
GACTTAGTGTCATGAAAAAA+AGG	+	chr7_2:56280676-56280695	None:intergenic	30.0%
CCAATTTACATTTAGTTAGG+AGG	+	chr7_2:56280785-56280804	None:intergenic	30.0%
CCTCCTAACTAAATGTAAAT+TGG	-	chr7_2:56280782-56280801	Msa1063980:intron	30.0%
CCACCAATTTACATTTAGTT+AGG	+	chr7_2:56280788-56280807	None:intergenic	30.0%
CCTAACTAAATGTAAATTGG+TGG	-	chr7_2:56280785-56280804	Msa1063980:intron	30.0%
AAGTTAAACCAATGTTTAGG+GGG	-	chr7_2:56280860-56280879	Msa1063980:intron	30.0%
CACTTTAATAAAACAAGCAG+AGG	-	chr7_2:56281069-56281088	Msa1063980:intron	30.0%
TTAATAAAACAAGCAGAGGA+TGG	-	chr7_2:56281073-56281092	Msa1063980:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	56280254	56282783	56280254	ID=Msa1063980;Name=Msa1063980
chr7_2	mRNA	56280254	56282783	56280254	ID=Msa1063980-mRNA-1;Parent=Msa1063980;Name=Msa1063980-mRNA-1;_AED=0.26;_eAED=0.26;_QI=15\|0.62\|0.66\|1\|0.75\|0.77\|9\|0\|246
chr7_2	exon	56280254	56280500	56280254	ID=Msa1063980-mRNA-1:exon:10607;Parent=Msa1063980-mRNA-1
chr7_2	exon	56280657	56280733	56280657	ID=Msa1063980-mRNA-1:exon:10606;Parent=Msa1063980-mRNA-1
chr7_2	exon	56280817	56280852	56280817	ID=Msa1063980-mRNA-1:exon:10605;Parent=Msa1063980-mRNA-1
chr7_2	exon	56280986	56281034	56280986	ID=Msa1063980-mRNA-1:exon:10604;Parent=Msa1063980-mRNA-1
chr7_2	exon	56281141	56281191	56281141	ID=Msa1063980-mRNA-1:exon:10603;Parent=Msa1063980-mRNA-1
chr7_2	exon	56281288	56281340	56281288	ID=Msa1063980-mRNA-1:exon:10602;Parent=Msa1063980-mRNA-1
chr7_2	exon	56281735	56281824	56281735	ID=Msa1063980-mRNA-1:exon:10601;Parent=Msa1063980-mRNA-1
chr7_2	exon	56281905	56281990	56281905	ID=Msa1063980-mRNA-1:exon:10600;Parent=Msa1063980-mRNA-1
chr7_2	exon	56282720	56282783	56282720	ID=Msa1063980-mRNA-1:exon:10599;Parent=Msa1063980-mRNA-1
chr7_2	five_prime_UTR	56282769	56282783	56282769	ID=Msa1063980-mRNA-1:five_prime_utr;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56282720	56282768	56282720	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56281905	56281990	56281905	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56281735	56281824	56281735	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56281288	56281340	56281288	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56281141	56281191	56281141	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56280986	56281034	56280986	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56280817	56280852	56280817	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56280657	56280733	56280657	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1
chr7_2	CDS	56280254	56280500	56280254	ID=Msa1063980-mRNA-1:cds;Parent=Msa1063980-mRNA-1

Gene Sequence

>Msa1063980

ATGACAAAATGCAACAGAACTGGAACTCAAACCCCTGGCTCAAATATTGGCCTGTTGACTAAAAAGCTCATCACTTTAATAAAACAAGCAGAGGATGGTATTCTTGATCTAAATAATGCTGCTGACACATTAGAGGTGCAAAAGAGGAGGATATATGATATTACTAATGTTCTTGAAGGGATTGGCCTTATAGAAAAAAAAGTTCAAGAACAGAATTCAGTGGAAGCAGAAATTGAAAATTTAACAATTGAGGAACGCCGGTTGGATGAACAAATAAGGGAGATGCAAGAAAGACTACGGAATCTCAGTGAAGAAGAAAATAATGAGAAGTTTCTTTTTGTCACTGAGGAAGATATAAAGAACTTGCCCTGCTTCCAGCACGGTACCACTTTGGAAGTACCTGATCCTGATGAGGCTGTTGATTATCCCCAGAGAAGATACAGGATAGTCTTGAGAAGCACAATGGGCCCAATGGATGTTTACCTTGTTAGTCAATTTGAAGAGAAGTTTGAGGAGATCAACGGTGTTGATGTTGCACCCAAATTTCCACCCAGTCCAGAAGTTGACAAGGATCAATCAACTGCGGTCCCAAAAGATAAAGGGAAGGAAATAGAAGTGCAGAGGCAAGATGGTGAAGGGCCTAGTTCAGATCTTACCAACAATCACGACTTCGTGAGTGGGATCATGAAGATTGTTCCTTCAGATGTTGCTGTAAGCATCACCTCAGTCTGGCGGATA

Protein sequence

>Msa1063980

MTKCNRTGTQTPGSNIGLLTKKLITLIKQAEDGILDLNNAADTLEVQKRRIYDITNVLEGIGLIEKKVQEQNSVEAEIENLTIEERRLDEQIREMQERLRNLSEEENNEKFLFVTEEDIKNLPCFQHGTTLEVPDPDEAVDYPQRRYRIVLRSTMGPMDVYLVSQFEEKFEEINGVDVAPKFPPSPEVDKDQSTAVPKDKGKEIEVQRQDGEGPSSDLTNNHDFVSGIMKIVPSDVAVSITSVWRI