AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1073970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1073970 MtrunA17_Chr7g0256981 92.835 321 16 2 1 321 1 314 0.0 533
Msa1073970 MtrunA17_Chr7g0257031 74.534 322 69 4 1 321 1 310 3.01e-145 411
Msa1073970 MtrunA17_Chr7g0257011 68.847 321 65 6 1 321 1 286 1.11e-130 374
Msa1073970 MtrunA17_Chr7g0256971 70.566 265 69 3 34 297 3 259 5.57e-118 340
Msa1073970 MtrunA17_Chr1g0172581 43.094 181 82 6 64 233 1 171 3.15e-37 131
Msa1073970 MtrunA17_Chr3g0085991 40.984 122 71 1 22 142 21 142 2.34e-29 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1073970 AT5G03990.1 48.428 159 72 3 1 154 1 154 5.27e-41 145
Msa1073970 AT3G51940.1 45.714 105 56 1 25 128 20 124 8.41e-29 115
Msa1073970 AT3G51940.2 50.000 92 45 1 38 128 37 128 1.72e-28 114
Msa1073970 AT5G03990.2 52.747 91 40 1 64 154 1 88 5.37e-24 98.6

Find 116 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATCCCAATGTGGTAAAT+TGG 0.166892 7_2:-71638925 Msa1073970:CDS
CAAAGGTGGCTTTCCTTTAT+AGG 0.179989 7_2:+71640459 None:intergenic
GAAATGGCGGTAATGGAAAT+TGG 0.216567 7_2:-71638394 Msa1073970:CDS
TAATTACTGAAATGGATAAT+TGG 0.271436 7_2:-71640540 Msa1073970:exon
TGTCGACCCCGAACTTTATC+TGG 0.292705 7_2:-71638771 Msa1073970:CDS
ATTAAGAAACTTTGGGAAAA+GGG 0.301759 7_2:+71640639 None:intergenic
GGCGAGGCAGCCGTGATTCT+TGG 0.305461 7_2:-71638704 Msa1073970:CDS
GGAGGAGGAGGAAGAGGTGG+AGG 0.309961 7_2:-71638239 Msa1073970:CDS
GGAGGAGGAAGAGGTGGAGG+AGG 0.311785 7_2:-71638236 Msa1073970:CDS
GTTGAACCTTCAAAGGAATA+TGG 0.318838 7_2:-71638500 Msa1073970:CDS
CAGATGGCTTTATTGTCTAT+TGG 0.319056 7_2:-71637987 Msa1073970:three_prime_UTR
GGTGGAGGAGGAGGAAGGAG+GGG 0.319614 7_2:-71638224 Msa1073970:CDS
AGAGGAAGAGGAGGAGGAGG+AGG 0.325244 7_2:-71638251 Msa1073970:CDS
AGGTGGAGGAGGAGGAAGGA+GGG 0.326176 7_2:-71638225 Msa1073970:CDS
CGCCGCAACATGGAAGAAAA+AGG 0.329418 7_2:-71638725 Msa1073970:CDS
GGAGGAAGAGGTGGAGGAGG+AGG 0.330303 7_2:-71638233 Msa1073970:CDS
TGACCCTTTAGCAGCTTCAT+AGG 0.335346 7_2:+71638119 None:intergenic
CGCCAACCATATTCCTTTGA+AGG 0.340454 7_2:+71638494 None:intergenic
GGATGGGGACCTACTGGATG+GGG 0.344597 7_2:-71638644 Msa1073970:CDS
TCCATCCATGATAATCATTC+TGG 0.346408 7_2:+71638465 None:intergenic
GAGGTGGAGGAGGAGGAAGG+AGG 0.349043 7_2:-71638226 Msa1073970:CDS
AAATGGCGGTAATGGAAATT+GGG 0.360900 7_2:-71638393 Msa1073970:CDS
AGAAGAAGAAGCGGAGGAAG+AGG 0.362675 7_2:-71638269 Msa1073970:CDS
GATGGGGACCTACTGGATGG+GGG 0.369719 7_2:-71638643 Msa1073970:CDS
GGAAGAGGAAGAGGAGGAGG+AGG 0.369969 7_2:-71638254 Msa1073970:CDS
AAATCCTCATCGTCCTATAA+AGG 0.372642 7_2:-71640472 Msa1073970:CDS
ATTATATCATATAAGAAGTA+TGG 0.374963 7_2:-71638037 Msa1073970:three_prime_UTR
TCCTTTGAAGGTTCAACAAC+AGG 0.379188 7_2:+71638506 None:intergenic
TTCCACATCCAGATAAAGTT+CGG 0.380635 7_2:+71638763 None:intergenic
GGAGGAAGAGGAAGAGGAGG+AGG 0.382208 7_2:-71638257 Msa1073970:CDS
TCCACATCCAGATAAAGTTC+GGG 0.382465 7_2:+71638764 None:intergenic
AATTAAGAAACTTTGGGAAA+AGG 0.384483 7_2:+71640638 None:intergenic
CTATAAAGGAAAGCCACCTT+TGG 0.390395 7_2:-71640458 Msa1073970:CDS
AAGGAATATGGTTGGCGAAA+TGG 0.391675 7_2:-71638488 Msa1073970:CDS
CTGGATGGGGACCTACTGGA+TGG 0.396986 7_2:-71638646 Msa1073970:CDS
AGAAGCGGAGGAAGAGGAAG+AGG 0.404491 7_2:-71638263 Msa1073970:CDS
GAAGAGGTGGAGGAGGAGGA+AGG 0.407371 7_2:-71638229 Msa1073970:CDS
CAAGGAAGAAATGGCGGTAA+TGG 0.409545 7_2:-71638401 Msa1073970:CDS
TGGATGGGGACCTACTGGAT+GGG 0.411862 7_2:-71638645 Msa1073970:CDS
TGAACAGAATTATGCTGCTC+AGG 0.411888 7_2:-71638588 Msa1073970:CDS
AACAAGTATCAAGGAAGAAA+TGG 0.421942 7_2:-71638410 Msa1073970:CDS
TCATCCCAATTTACCACATT+GGG 0.428459 7_2:+71638920 None:intergenic
AAAATAATGAAACAAACTCT+TGG 0.438543 7_2:-71638544 Msa1073970:CDS
TGGAATCAGACGGAGCAATA+TGG 0.440258 7_2:-71638446 Msa1073970:CDS
GGTTTCCAACAGTGCCTTTG+TGG 0.441064 7_2:-71639021 Msa1073970:CDS
ATCATCATCAAGTGGGTCAC+TGG 0.445627 7_2:-71640495 Msa1073970:CDS
TCCCCATCCAGGACCGGAAA+AGG 0.450165 7_2:+71638658 None:intergenic
ATGGGGACCTACTGGATGGG+GGG 0.451326 7_2:-71638642 Msa1073970:CDS
CCATGGCGAAAGGTAATAGA+AGG 0.451419 7_2:-71638965 Msa1073970:CDS
GGTCCTGGATGGGGACCTAC+TGG 0.453239 7_2:-71638650 Msa1073970:CDS
CTCACATTGATGATGTAGAT+TGG 0.455451 7_2:-71638802 Msa1073970:CDS
AGCGGAGGAAGAGGAAGAGG+AGG 0.458431 7_2:-71638260 Msa1073970:CDS
TAAATGAAGATCAAGTGCTT+TGG 0.464392 7_2:-71638158 Msa1073970:three_prime_UTR
ATTAGTGCCATGATGATGTC+CGG 0.466379 7_2:+71638313 None:intergenic
GATGATGATTACTGATCCCT+TGG 0.467657 7_2:+71640509 None:intergenic
TATAAAGGAAAGCCACCTTT+GGG 0.476143 7_2:-71640457 Msa1073970:intron
AAATAATGAAACAAACTCTT+GGG 0.477297 7_2:-71638543 Msa1073970:CDS
TCAACAACAGGAGCACGATA+TGG 0.478493 7_2:+71638518 None:intergenic
AATGAATATCAGATGAATAG+AGG 0.493050 7_2:-71638293 Msa1073970:CDS
GACTCCTATGAAGCTGCTAA+AGG 0.497038 7_2:-71638123 Msa1073970:three_prime_UTR
AGAATTATGCTGCTCAGGGA+TGG 0.497386 7_2:-71638583 Msa1073970:CDS
GCATCCCAATGTGGTAAATT+GGG 0.497460 7_2:-71638924 Msa1073970:CDS
TCCTGTTGTTGAACCTTCAA+AGG 0.497931 7_2:-71638507 Msa1073970:CDS
TCAATGTGAGTGTTGAGATC+AGG 0.498096 7_2:+71638815 None:intergenic
TTTCAGAGATAATTATATGA+AGG 0.501379 7_2:+71640696 None:intergenic
ACTCCTATGAAGCTGCTAAA+GGG 0.501415 7_2:-71638122 Msa1073970:three_prime_UTR
GTAATGGAAATTGGGGAGCA+TGG 0.502202 7_2:-71638385 Msa1073970:CDS
GTTTCCAACAGTGCCTTTGT+GGG 0.503784 7_2:-71639020 Msa1073970:CDS
ATCAGGCAATGGTATGTCGC+AGG 0.505403 7_2:+71638832 None:intergenic
GGAAATTGGGGAGCATGGGA+TGG 0.507278 7_2:-71638380 Msa1073970:CDS
TTAGTGCCATGATGATGTCC+GGG 0.507899 7_2:+71638314 None:intergenic
CATCCAGTAGGTCCCCATCC+AGG 0.508679 7_2:+71638647 None:intergenic
ATGATTCAGTACATTAGTAC+TGG 0.514710 7_2:-71637926 Msa1073970:three_prime_UTR
AGAGGAAGAAGAAGAAGCGG+AGG 0.514759 7_2:-71638275 Msa1073970:CDS
ATATGAAGGTGATGAGAAGA+AGG 0.520437 7_2:+71640710 None:intergenic
AATCGATTGGCTCAGTTCCA+TGG 0.520978 7_2:-71638982 Msa1073970:CDS
AGCAGAGGCCCGCCGCAACA+TGG 0.520992 7_2:-71638735 Msa1073970:CDS
AGTACTAATGTACTGAATCA+TGG 0.528010 7_2:+71637928 None:intergenic
GTCATCCCAATTTACCACAT+TGG 0.528139 7_2:+71638919 None:intergenic
TAATGGAAATTGGGGAGCAT+GGG 0.534866 7_2:-71638384 Msa1073970:CDS
CCCCGAACTTTATCTGGATG+TGG 0.535767 7_2:-71638765 Msa1073970:CDS
GTGGAAAAGGTAGCTACTCC+TGG 0.538528 7_2:-71638188 Msa1073970:CDS
CCTTCTATTACCTTTCGCCA+TGG 0.539041 7_2:+71638965 None:intergenic
ATCTTCATTTACCATGCACC+AGG 0.539123 7_2:+71638170 None:intergenic
GAATTATGCTGCTCAGGGAT+GGG 0.543052 7_2:-71638582 Msa1073970:CDS
GCCAGAATGATTATCATGGA+TGG 0.543243 7_2:-71638466 Msa1073970:CDS
GACTTGCATAACAAGTATCA+AGG 0.550052 7_2:-71638419 Msa1073970:CDS
AATAGAGGAAGAAGAAGAAG+CGG 0.550756 7_2:-71638278 Msa1073970:CDS
AACCTTCAAAGGAATATGGT+TGG 0.557337 7_2:-71638496 Msa1073970:CDS
AGTGCTTTGGACTGCATGCA+AGG 0.558385 7_2:-71638145 Msa1073970:three_prime_UTR
AATGGCCAGAATGATTATCA+TGG 0.560785 7_2:-71638470 Msa1073970:CDS
AGTAGGTCCCCATCCAGGAC+CGG 0.560984 7_2:+71638652 None:intergenic
AATAACCCCGGACATCATCA+TGG 0.580939 7_2:-71638320 Msa1073970:CDS
GAACAGAATTATGCTGCTCA+GGG 0.595487 7_2:-71638587 Msa1073970:CDS
GATCAGTAATCATCATCAAG+TGG 0.601542 7_2:-71640503 Msa1073970:CDS
CTTTCCTTTATAGGACGATG+AGG 0.606347 7_2:+71640468 None:intergenic
TATCACATATATAATATACC+TGG 0.613911 7_2:+71638059 None:intergenic
ATCAGTAATCATCATCAAGT+GGG 0.617268 7_2:-71640502 Msa1073970:CDS
AGGTAGCTACTCCTGGTGCA+TGG 0.624734 7_2:-71638181 Msa1073970:CDS
GGATGTGGAAAGAGAAGCAG+AGG 0.624882 7_2:-71638750 Msa1073970:CDS
TCAGGCAATGGTATGTCGCA+GGG 0.628570 7_2:+71638833 None:intergenic
TGGCTCAGTTCCATGGCGAA+AGG 0.630820 7_2:-71638975 Msa1073970:CDS
GGATGACTCTGCTGTCAAAG+AGG 0.633767 7_2:-71638903 Msa1073970:CDS
AAGAGAACTACCAAGAATCA+CGG 0.642265 7_2:+71638694 None:intergenic
AATGGCGGTAATGGAAATTG+GGG 0.644690 7_2:-71638392 Msa1073970:CDS
AGAGAGAAAACAATATGTCG+TGG 0.652185 7_2:-71638346 Msa1073970:CDS
AAGTATCAAGGAAGAAATGG+CGG 0.663489 7_2:-71638407 Msa1073970:CDS
AGGACAGAGGAAGCGTTCCG+AGG 0.663665 7_2:+71640789 None:intergenic
ATGTCGTGGTCTAATAACCC+CGG 0.670337 7_2:-71638332 Msa1073970:CDS
TGTGGAAGCAAGAACAACCT+CGG 0.672529 7_2:-71640806 None:intergenic
CCACATCCAGATAAAGTTCG+GGG 0.678042 7_2:+71638765 None:intergenic
GAATGGTACTAACCCAAAGG+TGG 0.681196 7_2:+71640445 None:intergenic
TTATCATGGATGGAATCAGA+CGG 0.682338 7_2:-71638456 Msa1073970:CDS
TAGTGCCATGATGATGTCCG+GGG 0.697507 7_2:+71638315 None:intergenic
TATGTATCTGCATCCCAATG+TGG 0.712444 7_2:-71638933 Msa1073970:CDS
TGAGTGTTGAGATCAGGCAA+TGG 0.737090 7_2:+71638821 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATTAAGAAACTTT+GGG + chr7_2:71638057-71638076 None:intergenic 10.0%
!! AAAAAATAATTAAGAAACTT+TGG + chr7_2:71638058-71638077 None:intergenic 10.0%
!!! TTTTCTTTTAGTTTATTTTA+AGG - chr7_2:71638364-71638383 Msa1073970:CDS 10.0%
!!! TTTTCTTTTAATTACTGAAA+TGG - chr7_2:71638138-71638157 Msa1073970:three_prime_UTR 15.0%
!! TAACAAATAAAACTAAAGAA+AGG + chr7_2:71638302-71638321 None:intergenic 15.0%
!! ATTTAATTATAGAAATGTAC+AGG - chr7_2:71638947-71638966 Msa1073970:CDS 15.0%
!! TTTAATTATAGAAATGTACA+GGG - chr7_2:71638948-71638967 Msa1073970:CDS 15.0%
!! AAATTTAACCAAACTTAAAA+TGG + chr7_2:71639212-71639231 None:intergenic 15.0%
!!! TATCTACTAATTAAATCTTT+TGG - chr7_2:71639401-71639420 Msa1073970:intron 15.0%
!! ATAAACACTTATTTATTGAA+TGG + chr7_2:71639524-71639543 None:intergenic 15.0%
!! ATTATATCATATAAGAAGTA+TGG - chr7_2:71640649-71640668 Msa1073970:five_prime_UTR 15.0%
!!! ATTTTGTAAAATTTATCAGA+TGG - chr7_2:71640683-71640702 Msa1073970:five_prime_UTR 15.0%
!! TTTCAGAGATAATTATATGA+AGG + chr7_2:71637993-71638012 None:intergenic 20.0%
!! TAATTACTGAAATGGATAAT+TGG - chr7_2:71638146-71638165 Msa1073970:three_prime_UTR 20.0%
!!! GATCAGTGATTTAAAATAAT+TGG - chr7_2:71638413-71638432 Msa1073970:CDS 20.0%
!! ATTGAACATATTCAAAAGTA+GGG + chr7_2:71638511-71638530 None:intergenic 20.0%
!! AATTGAACATATTCAAAAGT+AGG + chr7_2:71638512-71638531 None:intergenic 20.0%
!!! TACTTTTGAATATGTTCAAT+TGG - chr7_2:71638511-71638530 Msa1073970:CDS 20.0%
!!! ACTTTTGAATATGTTCAATT+GGG - chr7_2:71638512-71638531 Msa1073970:CDS 20.0%
!! TGAAATGTTGTAAAATATGA+TGG - chr7_2:71638752-71638771 Msa1073970:CDS 20.0%
!! CAAGATATTCAATAATTGAA+GGG - chr7_2:71638812-71638831 Msa1073970:CDS 20.0%
!! TTCTATAATTAAATGACGTA+GGG + chr7_2:71638941-71638960 None:intergenic 20.0%
!! TTTCTATAATTAAATGACGT+AGG + chr7_2:71638942-71638961 None:intergenic 20.0%
!!! AAATGAAACCATTTTAAGTT+TGG - chr7_2:71639201-71639220 Msa1073970:intron 20.0%
!!! AGAATCAATTGGTAATTTAA+GGG + chr7_2:71639617-71639636 None:intergenic 20.0%
!!! TAGAATCAATTGGTAATTTA+AGG + chr7_2:71639618-71639637 None:intergenic 20.0%
!! AAGGAATAAATAGAATCAAT+TGG + chr7_2:71639628-71639647 None:intergenic 20.0%
!!! TTGATTCTATTTATTCCTTT+TGG - chr7_2:71639629-71639648 Msa1073970:intron 20.0%
!!! TTGTTTCATTATTTTCATGT+TGG + chr7_2:71640137-71640156 None:intergenic 20.0%
!! AAAATAATGAAACAAACTCT+TGG - chr7_2:71640142-71640161 Msa1073970:intron 20.0%
!! AAATAATGAAACAAACTCTT+GGG - chr7_2:71640143-71640162 Msa1073970:intron 20.0%
!!! AAATTTTTCTTATGTGGAAA+AGG - chr7_2:71640485-71640504 Msa1073970:CDS 20.0%
!! TATCACATATATAATATACC+TGG + chr7_2:71640630-71640649 None:intergenic 20.0%
!! ATTAAGAAACTTTGGGAAAA+GGG + chr7_2:71638050-71638069 None:intergenic 25.0%
!! AATTAAGAAACTTTGGGAAA+AGG + chr7_2:71638051-71638070 None:intergenic 25.0%
! GTAATTAAAAGAAAAAGACC+CGG + chr7_2:71638133-71638152 None:intergenic 25.0%
! ATAATTGGAGAAAAAACCAA+GGG - chr7_2:71638161-71638180 Msa1073970:exon 25.0%
!! CTTTTGAATATGTTCAATTG+GGG - chr7_2:71638513-71638532 Msa1073970:CDS 25.0%
!!! TGGTTTTGAATGAACAATTT+TGG - chr7_2:71638555-71638574 Msa1073970:CDS 25.0%
!! ATTTTTCTTAGGATGAGTTT+TGG - chr7_2:71638626-71638645 Msa1073970:CDS 25.0%
! AATGTTGTAAAATATGATGG+AGG - chr7_2:71638755-71638774 Msa1073970:CDS 25.0%
!! ATGTTGTAAAATATGATGGA+GGG - chr7_2:71638756-71638775 Msa1073970:CDS 25.0%
! CCTTCAATTATTGAATATCT+TGG + chr7_2:71638814-71638833 None:intergenic 25.0%
! CCAAGATATTCAATAATTGA+AGG - chr7_2:71638811-71638830 Msa1073970:CDS 25.0%
!! ATGAATTGTTGTCATTTCTT+TGG - chr7_2:71638850-71638869 Msa1073970:CDS 25.0%
! ACAAAAGATAGACTAAAGAA+AGG + chr7_2:71639181-71639200 None:intergenic 25.0%
!!! GGTTAAATTTGTTCTGAAAA+TGG - chr7_2:71639222-71639241 Msa1073970:intron 25.0%
! ACAGGAAATTTAAGTATCTT+TGG + chr7_2:71639339-71639358 None:intergenic 25.0%
!! AAGAGTGTAGTTAAATAAAC+AGG + chr7_2:71639357-71639376 None:intergenic 25.0%
!! CTACTAATTAAATCTTTTGG+AGG - chr7_2:71639404-71639423 Msa1073970:intron 25.0%
!!! TTACTTTTCAAAGTATCCTT+AGG - chr7_2:71639432-71639451 Msa1073970:intron 25.0%
! TTCAATAATTGAATGACCTA+AGG + chr7_2:71639451-71639470 None:intergenic 25.0%
! AGGTCATTCAATTATTGAAT+AGG - chr7_2:71639452-71639471 Msa1073970:intron 25.0%
! AATGAATATCAGATGAATAG+AGG - chr7_2:71640393-71640412 Msa1073970:intron 25.0%
!!! GGATATAAATTTTGTTATCC+AGG - chr7_2:71640609-71640628 Msa1073970:five_prime_UTR 25.0%
!!! TAGTGTATGAAATGTTTTGA+TGG - chr7_2:71640731-71640750 Msa1073970:five_prime_UTR 25.0%
ATGATGTTGGAACAAAAACA+AGG + chr7_2:71637920-71637939 None:intergenic 30.0%
GATAATTGGAGAAAAAACCA+AGG - chr7_2:71638160-71638179 Msa1073970:exon 30.0%
ATCAGTAATCATCATCAAGT+GGG - chr7_2:71638184-71638203 Msa1073970:CDS 30.0%
! TAAAATATGATGGAGGGTTT+AGG - chr7_2:71638762-71638781 Msa1073970:CDS 30.0%
! AGGTTGTAATTTCTTGTTGT+TGG - chr7_2:71638886-71638905 Msa1073970:CDS 30.0%
! AACTATGTTTCTGTTTGTGT+TGG - chr7_2:71638980-71638999 Msa1073970:CDS 30.0%
GTCAATTTAATGATGAGGTA+GGG + chr7_2:71639135-71639154 None:intergenic 30.0%
ATTAAATTGACGAAGCTTAC+AGG - chr7_2:71639144-71639163 Msa1073970:intron 30.0%
!! TCTGAAAATGGTCTTGATTT+TGG - chr7_2:71639234-71639253 Msa1073970:intron 30.0%
!! ATGTTCAAGTTGGTATCTAT+TGG - chr7_2:71639259-71639278 Msa1073970:intron 30.0%
TCAATGATGACACACATTAT+CGG + chr7_2:71639300-71639319 None:intergenic 30.0%
TTCGTGTATTGAAAATGTCT+AGG - chr7_2:71639378-71639397 Msa1073970:intron 30.0%
! CTAGACAGATTTAGTTCAAA+AGG + chr7_2:71639495-71639514 None:intergenic 30.0%
!! AAGAAGTTTTGTGAATCGAT+TGG - chr7_2:71639691-71639710 Msa1073970:intron 30.0%
CATTTGACAATGCAAAAAAC+AGG - chr7_2:71639806-71639825 Msa1073970:intron 30.0%
! ACAATGCAAAAAACAGGTTT+TGG - chr7_2:71639812-71639831 Msa1073970:intron 30.0%
! CAATGCAAAAAACAGGTTTT+GGG - chr7_2:71639813-71639832 Msa1073970:intron 30.0%
AACAAGTATCAAGGAAGAAA+TGG - chr7_2:71640276-71640295 Msa1073970:intron 30.0%
TAAATGAAGATCAAGTGCTT+TGG - chr7_2:71640528-71640547 Msa1073970:CDS 30.0%
AGTACTAATGTACTGAATCA+TGG + chr7_2:71640761-71640780 None:intergenic 30.0%
ATGATTCAGTACATTAGTAC+TGG - chr7_2:71640760-71640779 Msa1073970:five_prime_UTR 30.0%
! GATGATGATGATGATGATGT+TGG + chr7_2:71637933-71637952 None:intergenic 35.0%
ATATGAAGGTGATGAGAAGA+AGG + chr7_2:71637979-71637998 None:intergenic 35.0%
! GCTTTTTGAATTTCCTCATC+CGG - chr7_2:71638111-71638130 Msa1073970:three_prime_UTR 35.0%
! CTTTTTGAATTTCCTCATCC+GGG - chr7_2:71638112-71638131 Msa1073970:three_prime_UTR 35.0%
GATCAGTAATCATCATCAAG+TGG - chr7_2:71638183-71638202 Msa1073970:CDS 35.0%
AAATCCTCATCGTCCTATAA+AGG - chr7_2:71638214-71638233 Msa1073970:CDS 35.0%
TATAAAGGAAAGCCACCTTT+GGG - chr7_2:71638229-71638248 Msa1073970:CDS 35.0%
ATTCAACTGCAACTCAATTG+TGG + chr7_2:71638461-71638480 None:intergenic 35.0%
AATATGTTCAATTGGGGTGT+TGG - chr7_2:71638519-71638538 Msa1073970:CDS 35.0%
!! TGGGGTGTTGGTTTAAAAAT+TGG - chr7_2:71638531-71638550 Msa1073970:CDS 35.0%
!! GTGTTGGTTTAAAAATTGGC+TGG - chr7_2:71638535-71638554 Msa1073970:CDS 35.0%
!!! CAATTTTGGTTTTGTGCTAG+TGG - chr7_2:71638569-71638588 Msa1073970:CDS 35.0%
!!! TAGGATGAGTTTTGGTTTGA+TGG - chr7_2:71638634-71638653 Msa1073970:CDS 35.0%
!!! CTTGGTCATTTTTGTTGACA+AGG + chr7_2:71638796-71638815 None:intergenic 35.0%
AATTGAAGGGAGTTTCACTA+AGG - chr7_2:71638825-71638844 Msa1073970:CDS 35.0%
! ATTGTTGTCATTTCTTTGGC+TGG - chr7_2:71638854-71638873 Msa1073970:CDS 35.0%
CAATGTGTCACATCAAGATT+CGG + chr7_2:71639104-71639123 None:intergenic 35.0%
CGTCAATTTAATGATGAGGT+AGG + chr7_2:71639136-71639155 None:intergenic 35.0%
GCTTCGTCAATTTAATGATG+AGG + chr7_2:71639140-71639159 None:intergenic 35.0%
!! GATTTTGGCAATGTTCAAGT+TGG - chr7_2:71639249-71639268 Msa1073970:intron 35.0%
AATGATGACACACATTATCG+GGG + chr7_2:71639298-71639317 None:intergenic 35.0%
CAATGATGACACACATTATC+GGG + chr7_2:71639299-71639318 None:intergenic 35.0%
! CTTTTGCATGTTTTCCTTCT+AGG - chr7_2:71639575-71639594 Msa1073970:intron 35.0%
TTCCTTCTAGGTGTGATTAT+AGG - chr7_2:71639587-71639606 Msa1073970:intron 35.0%
AACCTATAATCACACCTAGA+AGG + chr7_2:71639592-71639611 None:intergenic 35.0%
! CAAAACTTCTTTTCCCACAA+AGG + chr7_2:71639682-71639701 None:intergenic 35.0%
TCATCCCAATTTACCACATT+GGG + chr7_2:71639769-71639788 None:intergenic 35.0%
!! CTCACATTGATGATGTAGAT+TGG - chr7_2:71639884-71639903 Msa1073970:intron 35.0%
TTCCACATCCAGATAAAGTT+CGG + chr7_2:71639926-71639945 None:intergenic 35.0%
AAGAGAACTACCAAGAATCA+CGG + chr7_2:71639995-71640014 None:intergenic 35.0%
GTTGAACCTTCAAAGGAATA+TGG - chr7_2:71640186-71640205 Msa1073970:intron 35.0%
AACCTTCAAAGGAATATGGT+TGG - chr7_2:71640190-71640209 Msa1073970:intron 35.0%
AATGGCCAGAATGATTATCA+TGG - chr7_2:71640216-71640235 Msa1073970:intron 35.0%
TCCATCCATGATAATCATTC+TGG + chr7_2:71640224-71640243 None:intergenic 35.0%
TTATCATGGATGGAATCAGA+CGG - chr7_2:71640230-71640249 Msa1073970:intron 35.0%
GACTTGCATAACAAGTATCA+AGG - chr7_2:71640267-71640286 Msa1073970:intron 35.0%
AAGTATCAAGGAAGAAATGG+CGG - chr7_2:71640279-71640298 Msa1073970:intron 35.0%
!! AAATGGCGGTAATGGAAATT+GGG - chr7_2:71640293-71640312 Msa1073970:intron 35.0%
AGAGAGAAAACAATATGTCG+TGG - chr7_2:71640340-71640359 Msa1073970:intron 35.0%
AATAGAGGAAGAAGAAGAAG+CGG - chr7_2:71640408-71640427 Msa1073970:intron 35.0%
! GAGGGGAAATTTTTCTTATG+TGG - chr7_2:71640479-71640498 Msa1073970:CDS 35.0%
!! CAGATGGCTTTATTGTCTAT+TGG - chr7_2:71640699-71640718 Msa1073970:five_prime_UTR 35.0%
TGGAACAAAAACAAGGACAG+AGG + chr7_2:71637913-71637932 None:intergenic 40.0%
AAAAGAAAAAGACCCGGATG+AGG + chr7_2:71638127-71638146 None:intergenic 40.0%
GATGATGATTACTGATCCCT+TGG + chr7_2:71638180-71638199 None:intergenic 40.0%
CTTTCCTTTATAGGACGATG+AGG + chr7_2:71638221-71638240 None:intergenic 40.0%
CAAAGGTGGCTTTCCTTTAT+AGG + chr7_2:71638230-71638249 None:intergenic 40.0%
CTATAAAGGAAAGCCACCTT+TGG - chr7_2:71638228-71638247 Msa1073970:CDS 40.0%
AGAGAATGGTACTAACCCAA+AGG + chr7_2:71638247-71638266 None:intergenic 40.0%
!!! TTTGGTTTTGTGCTAGTGGT+AGG - chr7_2:71638573-71638592 Msa1073970:CDS 40.0%
TAAGCCTCTTCTCACAAAAG+AGG + chr7_2:71638679-71638698 None:intergenic 40.0%
! TTGTCATTTCTTTGGCTGGT+GGG - chr7_2:71638858-71638877 Msa1073970:CDS 40.0%
! TGGTGTTTTGCATGATCATC+AGG - chr7_2:71638906-71638925 Msa1073970:CDS 40.0%
! GTTTTGCATGATCATCAGGT+TGG - chr7_2:71638910-71638929 Msa1073970:CDS 40.0%
! CTTTGATTTCATGTCGTCTG+TGG - chr7_2:71639048-71639067 Msa1073970:intron 40.0%
ATGATGACACACATTATCGG+GGG + chr7_2:71639297-71639316 None:intergenic 40.0%
CCTATTATCTGCGAACCAAA+AGG + chr7_2:71639647-71639666 None:intergenic 40.0%
!!! CCTTTTGGTTCGCAGATAAT+AGG - chr7_2:71639644-71639663 Msa1073970:intron 40.0%
TATGTATCTGCATCCCAATG+TGG - chr7_2:71639753-71639772 Msa1073970:intron 40.0%
TGCATCCCAATGTGGTAAAT+TGG - chr7_2:71639761-71639780 Msa1073970:intron 40.0%
GCATCCCAATGTGGTAAATT+GGG - chr7_2:71639762-71639781 Msa1073970:intron 40.0%
GTCATCCCAATTTACCACAT+TGG + chr7_2:71639770-71639789 None:intergenic 40.0%
!! TCAATGTGAGTGTTGAGATC+AGG + chr7_2:71639874-71639893 None:intergenic 40.0%
TCCACATCCAGATAAAGTTC+GGG + chr7_2:71639925-71639944 None:intergenic 40.0%
! CTCTTGAATCAGTCCTTTTC+CGG - chr7_2:71640015-71640034 Msa1073970:intron 40.0%
TGAACAGAATTATGCTGCTC+AGG - chr7_2:71640098-71640117 Msa1073970:intron 40.0%
GAACAGAATTATGCTGCTCA+GGG - chr7_2:71640099-71640118 Msa1073970:intron 40.0%
TCCTGTTGTTGAACCTTCAA+AGG - chr7_2:71640179-71640198 Msa1073970:intron 40.0%
! TCCTTTGAAGGTTCAACAAC+AGG + chr7_2:71640183-71640202 None:intergenic 40.0%
AAGGAATATGGTTGGCGAAA+TGG - chr7_2:71640198-71640217 Msa1073970:intron 40.0%
GCCAGAATGATTATCATGGA+TGG - chr7_2:71640220-71640239 Msa1073970:intron 40.0%
!! GAAATGGCGGTAATGGAAAT+TGG - chr7_2:71640292-71640311 Msa1073970:intron 40.0%
!! AATGGCGGTAATGGAAATTG+GGG - chr7_2:71640294-71640313 Msa1073970:intron 40.0%
! TAATGGAAATTGGGGAGCAT+GGG - chr7_2:71640302-71640321 Msa1073970:intron 40.0%
! ATTAGTGCCATGATGATGTC+CGG + chr7_2:71640376-71640395 None:intergenic 40.0%
ATCTTCATTTACCATGCACC+AGG + chr7_2:71640519-71640538 None:intergenic 40.0%
ACTCCTATGAAGCTGCTAAA+GGG - chr7_2:71640564-71640583 Msa1073970:five_prime_UTR 40.0%
!! CAGTTTTGTGAAAGTAGCTG+AGG - chr7_2:71640588-71640607 Msa1073970:five_prime_UTR 40.0%
ATCATCATCAAGTGGGTCAC+TGG - chr7_2:71638191-71638210 Msa1073970:CDS 45.0%
GAATGGTACTAACCCAAAGG+TGG + chr7_2:71638244-71638263 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAGAA+TGG + chr7_2:71638261-71638280 None:intergenic 45.0%
! CATGCCTCTTTTGTGAGAAG+AGG - chr7_2:71638672-71638691 Msa1073970:CDS 45.0%
TGTGTGTGACTCATGTGACA+GGG - chr7_2:71638726-71638745 Msa1073970:CDS 45.0%
! GTTGTCATTTCTTTGGCTGG+TGG - chr7_2:71638857-71638876 Msa1073970:CDS 45.0%
!! TGCATGATCATCAGGTTGGT+TGG - chr7_2:71638914-71638933 Msa1073970:CDS 45.0%
GTTTCCAACAGTGCCTTTGT+GGG - chr7_2:71639666-71639685 Msa1073970:intron 45.0%
!!! TTTTCCCACAAAGGCACTGT+TGG + chr7_2:71639673-71639692 None:intergenic 45.0%
! AATCGATTGGCTCAGTTCCA+TGG - chr7_2:71639704-71639723 Msa1073970:intron 45.0%
CCTTCTATTACCTTTCGCCA+TGG + chr7_2:71639724-71639743 None:intergenic 45.0%
CCATGGCGAAAGGTAATAGA+AGG - chr7_2:71639721-71639740 Msa1073970:intron 45.0%
!!! AGGTTTTGGGCTGAGATCAA+CGG - chr7_2:71639826-71639845 Msa1073970:intron 45.0%
!! TGAGTGTTGAGATCAGGCAA+TGG + chr7_2:71639868-71639887 None:intergenic 45.0%
CCACATCCAGATAAAGTTCG+GGG + chr7_2:71639924-71639943 None:intergenic 45.0%
! TTTTTCTTCCATGTTGCGGC+GGG + chr7_2:71639962-71639981 None:intergenic 45.0%
! CTTTTTCTTCCATGTTGCGG+CGG + chr7_2:71639963-71639982 None:intergenic 45.0%
! CGCCTTTTTCTTCCATGTTG+CGG + chr7_2:71639966-71639985 None:intergenic 45.0%
CAACATGGAAGAAAAAGGCG+AGG - chr7_2:71639966-71639985 Msa1073970:intron 45.0%
AGAATTATGCTGCTCAGGGA+TGG - chr7_2:71640103-71640122 Msa1073970:intron 45.0%
GAATTATGCTGCTCAGGGAT+GGG - chr7_2:71640104-71640123 Msa1073970:intron 45.0%
! TCAACAACAGGAGCACGATA+TGG + chr7_2:71640171-71640190 None:intergenic 45.0%
CGCCAACCATATTCCTTTGA+AGG + chr7_2:71640195-71640214 None:intergenic 45.0%
TGGAATCAGACGGAGCAATA+TGG - chr7_2:71640240-71640259 Msa1073970:intron 45.0%
CAAGGAAGAAATGGCGGTAA+TGG - chr7_2:71640285-71640304 Msa1073970:intron 45.0%
! GTAATGGAAATTGGGGAGCA+TGG - chr7_2:71640301-71640320 Msa1073970:intron 45.0%
ATGTCGTGGTCTAATAACCC+CGG - chr7_2:71640354-71640373 Msa1073970:intron 45.0%
AATAACCCCGGACATCATCA+TGG - chr7_2:71640366-71640385 Msa1073970:intron 45.0%
! TTAGTGCCATGATGATGTCC+GGG + chr7_2:71640375-71640394 None:intergenic 45.0%
GACTCCTATGAAGCTGCTAA+AGG - chr7_2:71640563-71640582 Msa1073970:five_prime_UTR 45.0%
TGACCCTTTAGCAGCTTCAT+AGG + chr7_2:71640570-71640589 None:intergenic 45.0%
!!! ATTTTAATTTAATTTTTCTT+AGG - chr7_2:71638615-71638634 Msa1073970:CDS 5.0%
GTGTGTGTGACTCATGTGAC+AGG - chr7_2:71638725-71638744 Msa1073970:CDS 50.0%
!! TCTTTGGCTGGTGGGTTGTT+AGG - chr7_2:71638866-71638885 Msa1073970:CDS 50.0%
GGTTTCCAACAGTGCCTTTG+TGG - chr7_2:71639665-71639684 Msa1073970:intron 50.0%
GGATGACTCTGCTGTCAAAG+AGG - chr7_2:71639783-71639802 Msa1073970:intron 50.0%
TCAGGCAATGGTATGTCGCA+GGG + chr7_2:71639856-71639875 None:intergenic 50.0%
ATCAGGCAATGGTATGTCGC+AGG + chr7_2:71639857-71639876 None:intergenic 50.0%
TGTCGACCCCGAACTTTATC+TGG - chr7_2:71639915-71639934 Msa1073970:intron 50.0%
CCCCGAACTTTATCTGGATG+TGG - chr7_2:71639921-71639940 Msa1073970:intron 50.0%
GGATGTGGAAAGAGAAGCAG+AGG - chr7_2:71639936-71639955 Msa1073970:intron 50.0%
CGCCGCAACATGGAAGAAAA+AGG - chr7_2:71639961-71639980 Msa1073970:intron 50.0%
! AATCAGTCCTTTTCCGGTCC+TGG - chr7_2:71640021-71640040 Msa1073970:intron 50.0%
! TAGTGCCATGATGATGTCCG+GGG + chr7_2:71640374-71640393 None:intergenic 50.0%
AGAGGAAGAAGAAGAAGCGG+AGG - chr7_2:71640411-71640430 Msa1073970:intron 50.0%
AGAAGAAGAAGCGGAGGAAG+AGG - chr7_2:71640417-71640436 Msa1073970:intron 50.0%
GTGGAAAAGGTAGCTACTCC+TGG - chr7_2:71640498-71640517 Msa1073970:CDS 50.0%
! AGTGCTTTGGACTGCATGCA+AGG - chr7_2:71640541-71640560 Msa1073970:exon 50.0%
TGGCTCAGTTCCATGGCGAA+AGG - chr7_2:71639711-71639730 Msa1073970:intron 55.0%
! AGTCCTTTTCCGGTCCTGGA+TGG - chr7_2:71640025-71640044 Msa1073970:intron 55.0%
! GTCCTTTTCCGGTCCTGGAT+GGG - chr7_2:71640026-71640045 Msa1073970:intron 55.0%
! TCCTTTTCCGGTCCTGGATG+GGG - chr7_2:71640027-71640046 Msa1073970:intron 55.0%
TGGATGGGGACCTACTGGAT+GGG - chr7_2:71640041-71640060 Msa1073970:intron 55.0%
! GGAAATTGGGGAGCATGGGA+TGG - chr7_2:71640306-71640325 Msa1073970:intron 55.0%
AGAAGCGGAGGAAGAGGAAG+AGG - chr7_2:71640423-71640442 Msa1073970:intron 55.0%
AGGTAGCTACTCCTGGTGCA+TGG - chr7_2:71640505-71640524 Msa1073970:CDS 55.0%
!! AGGACAGAGGAAGCGTTCCG+AGG + chr7_2:71637900-71637919 None:intergenic 60.0%
TCCCCATCCAGGACCGGAAA+AGG + chr7_2:71640031-71640050 None:intergenic 60.0%
AGTAGGTCCCCATCCAGGAC+CGG + chr7_2:71640037-71640056 None:intergenic 60.0%
CATCCAGTAGGTCCCCATCC+AGG + chr7_2:71640042-71640061 None:intergenic 60.0%
CTGGATGGGGACCTACTGGA+TGG - chr7_2:71640040-71640059 Msa1073970:intron 60.0%
GGATGGGGACCTACTGGATG+GGG - chr7_2:71640042-71640061 Msa1073970:intron 60.0%
GATGGGGACCTACTGGATGG+GGG - chr7_2:71640043-71640062 Msa1073970:intron 60.0%
ATGGGGACCTACTGGATGGG+GGG - chr7_2:71640044-71640063 Msa1073970:intron 60.0%
TCTTCGTCCCCCCATCCAGT+AGG + chr7_2:71640054-71640073 None:intergenic 60.0%
AGCGGAGGAAGAGGAAGAGG+AGG - chr7_2:71640426-71640445 Msa1073970:intron 60.0%
GGAGGAAGAGGAAGAGGAGG+AGG - chr7_2:71640429-71640448 Msa1073970:intron 60.0%
GGAAGAGGAAGAGGAGGAGG+AGG - chr7_2:71640432-71640451 Msa1073970:intron 60.0%
AGAGGAAGAGGAGGAGGAGG+AGG - chr7_2:71640435-71640454 Msa1073970:intron 60.0%
AGAGGAGGAGGAGGAGGAAG+AGG - chr7_2:71640441-71640460 Msa1073970:intron 60.0%
GAAGAGGTGGAGGAGGAGGA+AGG - chr7_2:71640457-71640476 Msa1073970:intron 60.0%
AGGTGGAGGAGGAGGAAGGA+GGG - chr7_2:71640461-71640480 Msa1073970:CDS 60.0%
GGCGAGGCAGCCGTGATTCT+TGG - chr7_2:71639982-71640001 Msa1073970:intron 65.0%
GGTCCTGGATGGGGACCTAC+TGG - chr7_2:71640036-71640055 Msa1073970:intron 65.0%
GGAGGAGGAGGAGGAAGAGG+TGG - chr7_2:71640444-71640463 Msa1073970:intron 65.0%
GGAGGAGGAGGAAGAGGTGG+AGG - chr7_2:71640447-71640466 Msa1073970:intron 65.0%
GGAGGAGGAAGAGGTGGAGG+AGG - chr7_2:71640450-71640469 Msa1073970:intron 65.0%
GGAGGAAGAGGTGGAGGAGG+AGG - chr7_2:71640453-71640472 Msa1073970:intron 65.0%
GAGGTGGAGGAGGAGGAAGG+AGG - chr7_2:71640460-71640479 Msa1073970:CDS 65.0%
GGTGGAGGAGGAGGAAGGAG+GGG - chr7_2:71640462-71640481 Msa1073970:CDS 65.0%
AGCAGAGGCCCGCCGCAACA+TGG - chr7_2:71639951-71639970 Msa1073970:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 71637891 71640817 71637891 ID=Msa1073970;Name=Msa1073970
chr7_2 mRNA 71637891 71640817 71637891 ID=Msa1073970-mRNA-1;Parent=Msa1073970;Name=Msa1073970-mRNA-1;_AED=0.19;_eAED=0.19;_QI=266|1|1|1|1|1|2|287|321
chr7_2 exon 71637891 71639049 71637891 ID=Msa1073970-mRNA-1:exon:16294;Parent=Msa1073970-mRNA-1
chr7_2 exon 71640458 71640817 71640458 ID=Msa1073970-mRNA-1:exon:16293;Parent=Msa1073970-mRNA-1
chr7_2 five_prime_UTR 71640552 71640817 71640552 ID=Msa1073970-mRNA-1:five_prime_utr;Parent=Msa1073970-mRNA-1
chr7_2 CDS 71640458 71640551 71640458 ID=Msa1073970-mRNA-1:cds;Parent=Msa1073970-mRNA-1
chr7_2 CDS 71638178 71639049 71638178 ID=Msa1073970-mRNA-1:cds;Parent=Msa1073970-mRNA-1
chr7_2 three_prime_UTR 71637891 71638177 71637891 ID=Msa1073970-mRNA-1:three_prime_utr;Parent=Msa1073970-mRNA-1
Gene Sequence

>Msa1073970

ATGGATAATTGGAGAAAAAACCAAGGGATCAGTAATCATCATCAAGTGGGTCACTGGAAATCCTCATCGTCCTATAAAGGAAAGCCACCTTTGGATAATAGGTTTCCAACAGTGCCTTTGTGGGAAAAGAAGTTTTGTGAATCGATTGGCTCAGTTCCATGGCGAAAGGTAATAGAAGGCAATAAGTATATGTATCTGCATCCCAATGTGGTAAATTGGGATGACTCTGCTGTCAAAGAGGCATTTGACAATGCAAAAAACAGGTTTTGGGCTGAGATCAACGGCATTCCCTGCGACATACCATTGCCTGATCTCAACACTCACATTGATGATGTAGATTGGAATGCAAGTGTCGACCCCGAACTTTATCTGGATGTGGAAAGAGAAGCAGAGGCCCGCCGCAACATGGAAGAAAAAGGCGAGGCAGCCGTGATTCTTGGTAGTTCTCTTCTCTTGAATCAGTCCTTTTCCGGTCCTGGATGGGGACCTACTGGATGGGGGGACGAAGAAGAAAAAGAAGTAACAAAGACTTCTGAACAGAATTATGCTGCTCAGGGATGGGAATTGAACCAACATGAAAATAATGAAACAAACTCTTGGGAACCATATCGTGCTCCTGTTGTTGAACCTTCAAAGGAATATGGTTGGCGAAATGGCCAGAATGATTATCATGGATGGAATCAGACGGAGCAATATGGTGCTGACTTGCATAACAAGTATCAAGGAAGAAATGGCGGTAATGGAAATTGGGGAGCATGGGATGGATATAACAGAAAGAGAGAAAACAATATGTCGTGGTCTAATAACCCCGGACATCATCATGGCACTAATGAATATCAGATGAATAGAGGAAGAAGAAGAAGCGGAGGAAGAGGAAGAGGAGGAGGAGGAGGAAGAGGTGGAGGAGGAGGAAGGAGGGGAAATTTTTCTTATGTGGAAAAGGTAGCTACTCCTGGTGCATGGTAA
Protein sequence

>Msa1073970

MDNWRKNQGISNHHQVGHWKSSSSYKGKPPLDNRFPTVPLWEKKFCESIGSVPWRKVIEGNKYMYLHPNVVNWDDSAVKEAFDNAKNRFWAEINGIPCDIPLPDLNTHIDDVDWNASVDPELYLDVEREAEARRNMEEKGEAAVILGSSLLLNQSFSGPGWGPTGWGDEEEKEVTKTSEQNYAAQGWELNQHENNETNSWEPYRAPVVEPSKEYGWRNGQNDYHGWNQTEQYGADLHNKYQGRNGGNGNWGAWDGYNRKRENNMSWSNNPGHHHGTNEYQMNRGRRRSGGRGRGGGGGRGGGGGRRGNFSYVEKVATPGAW*