AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1075860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1075860 MtrunA17_Chr7g0259691 100.000 115 0 0 1 115 1 115 7.06e-78 224
Msa1075860 MtrunA17_Chr1g0185641 99.130 115 0 1 1 115 1 114 1.97e-75 218
Msa1075860 MtrunA17_Chr8g0383391 28.070 114 76 2 4 114 9 119 7.00e-12 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1075860 AT4G02840.1 92.857 98 7 0 1 98 1 98 1.87e-61 184
Msa1075860 AT3G07590.2 91.579 95 8 0 1 95 1 95 8.36e-60 179
Msa1075860 AT3G07590.1 91.579 95 8 0 1 95 1 95 8.36e-60 179
Msa1075860 AT4G02840.2 84.259 108 7 1 1 98 1 108 3.29e-58 176
Msa1075860 AT3G07590.3 87.879 66 8 0 30 95 4 69 4.57e-38 124
Msa1075860 AT1G20580.1 29.245 106 72 1 4 109 9 111 3.22e-12 59.3

Find 53 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCCTTTGGGGCGAGGACG+GGG 0.284771 7_2:+74277015 Msa1075860:CDS
GACAGCTTCTTTCAGTTGAT+TGG 0.291608 7_2:-74277249 None:intergenic
CTGTCTTAGTAGGAGCTTAT+AGG 0.302229 7_2:+74277267 Msa1075860:three_prime_UTR
GTACCAGCAGTTGGCTTCTT+AGG 0.317812 7_2:-74276876 None:intergenic
CCCCTTTAGAGTTAGCTTAA+CGG 0.355184 7_2:-74276736 None:intergenic
ACCGTTAAGCTAACTCTAAA+GGG 0.355730 7_2:+74276735 Msa1075860:CDS
CTTCAACAAGTAAAGTTTCA+AGG 0.362204 7_2:-74276836 None:intergenic
CTGAGATGATCCAAAGTAAC+TGG 0.363042 7_2:-74276765 None:intergenic
CAGAAAATTGCCCTACAATA+AGG 0.371910 7_2:-74277104 None:intergenic
TATGGATTTGACCTTATTGT+AGG 0.377788 7_2:+74277093 Msa1075860:three_prime_UTR
ATTGCAGGAGGAAAGCCTTT+GGG 0.380608 7_2:+74277003 Msa1075860:intron
ACCTGTGATACACAATTTAA+CGG 0.381233 7_2:-74277068 None:intergenic
AAAGCCTTTGGGGCGAGGAC+GGG 0.388156 7_2:+74277014 Msa1075860:CDS
AAGAATGGTACTATTGTTCA+TGG 0.396668 7_2:+74273130 Msa1075860:CDS
AATTGTGTTATTTGAACAAA+TGG 0.406190 7_2:+74277332 Msa1075860:three_prime_UTR
GTTTCAAGGTTCAAGCTGTC+AGG 0.435597 7_2:-74276822 None:intergenic
CTGAAAGAAGCTGTCTTAGT+AGG 0.440471 7_2:+74277257 Msa1075860:three_prime_UTR
AATTGCAGGAGGAAAGCCTT+TGG 0.442871 7_2:+74277002 Msa1075860:intron
GAAAGCCTTTGGGGCGAGGA+CGG 0.445123 7_2:+74277013 Msa1075860:CDS
CGTGGTCGTGGACGCGGTCG+TGG 0.448245 7_2:+74277045 Msa1075860:CDS
AACCGTTAAGCTAACTCTAA+AGG 0.451979 7_2:+74276734 Msa1075860:CDS
TGCCTTGTCTGCAGTGGTAA+TGG 0.454058 7_2:+74277308 Msa1075860:three_prime_UTR
CGAGGACGTGGTCGTGGACG+CGG 0.454065 7_2:+74277039 Msa1075860:CDS
GTGATACCTAACGAGCTTCA+TGG 0.454978 7_2:-74272985 None:intergenic
CCACCACCATGAAGCTCGTT+AGG 0.465256 7_2:+74272979 Msa1075860:exon
ATCTAGTTTATAATCCCTGC+TGG 0.466639 7_2:+74277213 Msa1075860:three_prime_UTR
GGCATTTGACATTACCCAGC+AGG 0.468140 7_2:-74277228 None:intergenic
GCCGTTAAATTGTGTATCAC+AGG 0.473452 7_2:+74277067 Msa1075860:exon
AGCCTTTGGGGCGAGGACGG+GGG 0.474600 7_2:+74277016 Msa1075860:CDS
GGAGGAAAGCCTTTGGGGCG+AGG 0.495880 7_2:+74277009 Msa1075860:intron
TACTTGTTGAAGAAGCTCCT+AGG 0.498295 7_2:+74276847 Msa1075860:CDS
ACTTGTTGAAGAAGCTCCTA+GGG 0.502700 7_2:+74276848 Msa1075860:CDS
CACTAAACACTAGTGTAGTT+CGG 0.503209 7_2:+74272891 Msa1075860:five_prime_UTR
ATGGATTTGACCTTATTGTA+GGG 0.504921 7_2:+74277094 Msa1075860:three_prime_UTR
CCTAACGAGCTTCATGGTGG+TGG 0.519786 7_2:-74272979 None:intergenic
GGCTTCTTAGGCTTGACCCT+AGG 0.525010 7_2:-74276864 None:intergenic
GTCTCAATCGAGTTGAAGAA+TGG 0.539433 7_2:+74273115 Msa1075860:CDS
ATTGTTCATGGCACTATCAC+AGG 0.543560 7_2:+74273142 Msa1075860:CDS
AAGCCTAAGAAGCCAACTGC+TGG 0.548844 7_2:+74276873 Msa1075860:CDS
GTGTGTTATTGTTAGTTGTG+TGG 0.568402 7_2:+74277148 Msa1075860:three_prime_UTR
TTGCAGGAGGAAAGCCTTTG+GGG 0.577723 7_2:+74277004 Msa1075860:intron
TACCATTACCACTGCAGACA+AGG 0.595627 7_2:-74277310 None:intergenic
GCATTTGACATTACCCAGCA+GGG 0.597689 7_2:-74277227 None:intergenic
ATTCTTCAACTCGATTGAGA+CGG 0.606708 7_2:-74273113 None:intergenic
TTGGATCATCTCAGTGTGAG+AGG 0.610982 7_2:+74276774 Msa1075860:CDS
CAAGGTTCAAGCTGTCAGGA+AGG 0.613711 7_2:-74276818 None:intergenic
TCTAGTTTATAATCCCTGCT+GGG 0.621220 7_2:+74277214 Msa1075860:three_prime_UTR
CCGTTAAGCTAACTCTAAAG+GGG 0.635466 7_2:+74276736 Msa1075860:CDS
CTGACTTGCCTTGTCTGCAG+TGG 0.640301 7_2:+74277302 Msa1075860:three_prime_UTR
TCGTATACAGTACCAGCAGT+TGG 0.655537 7_2:-74276885 None:intergenic
AAATAGCGGCAGAAAGAGTG+AGG 0.656474 7_2:-74272927 None:intergenic
CGGTGGAAAATCGCAAATAG+CGG 0.672058 7_2:-74272941 None:intergenic
ATACCTAACGAGCTTCATGG+TGG 0.754248 7_2:-74272982 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAATAATAATTA+GGG - chr7_2:74273249-74273268 None:intergenic 0.0%
!! TAATAATAATAATAATAATT+AGG - chr7_2:74273250-74273269 None:intergenic 0.0%
!! ATAATAATAATAGAGAAAAT+AGG - chr7_2:74273443-74273462 None:intergenic 10.0%
!!! TAAAAAAATATTTTGCATTT+TGG - chr7_2:74274264-74274283 None:intergenic 10.0%
!! AAAGTCAATATATAAAAATA+GGG - chr7_2:74275701-74275720 None:intergenic 10.0%
!! AAAAGTCAATATATAAAAAT+AGG - chr7_2:74275702-74275721 None:intergenic 10.0%
!! GGATAAAAAAAAATACATTT+CGG - chr7_2:74274201-74274220 None:intergenic 15.0%
!!! AAGCAGTTTTTTTTTAAATA+AGG - chr7_2:74274346-74274365 None:intergenic 15.0%
!! TACATAAATAAGAAAAGAAA+TGG - chr7_2:74274590-74274609 None:intergenic 15.0%
!! TGATATTAATTAATGAATCA+TGG + chr7_2:74275124-74275143 Msa1075860:intron 15.0%
!!! TGGTAAATATTTTAATCTTA+TGG - chr7_2:74275276-74275295 None:intergenic 15.0%
!!! ATAAGCTGTTTTTTAAAATA+AGG + chr7_2:74275324-74275343 Msa1075860:intron 15.0%
!!! ATTGAAAACAACTTATTTTA+TGG - chr7_2:74275356-74275375 None:intergenic 15.0%
!!! TAAGTTGTTTTCATAAAATA+AGG + chr7_2:74275440-74275459 Msa1075860:intron 15.0%
!!! TTTCAAATAAGTTGTTTTTT+TGG + chr7_2:74275498-74275517 Msa1075860:intron 15.0%
!! AAGAACTGTTAATAAATTAA+CGG + chr7_2:74275581-74275600 Msa1075860:intron 15.0%
!! AAGTCAATATATAAAAATAG+GGG - chr7_2:74275700-74275719 None:intergenic 15.0%
!! TTTAAATACTTTCTTATTCT+TGG + chr7_2:74275880-74275899 Msa1075860:intron 15.0%
!! AAGATTACCAAAAAAATATA+CGG - chr7_2:74276074-74276093 None:intergenic 15.0%
!!! TACAAATTATGTTTTGATTA+TGG + chr7_2:74276098-74276117 Msa1075860:intron 15.0%
!! ACAATTAGTATACAAAAATA+TGG - chr7_2:74276149-74276168 None:intergenic 15.0%
!! TATTATTACTACGAGTTTAA+TGG + chr7_2:74273262-74273281 Msa1075860:intron 20.0%
!! GAAGTTGTTCTTAATATTAT+AGG - chr7_2:74273606-74273625 None:intergenic 20.0%
!! AATTAAGAAAAAAGGGATTA+GGG - chr7_2:74273942-74273961 None:intergenic 20.0%
!! AAATTAAGAAAAAAGGGATT+AGG - chr7_2:74273943-74273962 None:intergenic 20.0%
!!! TTTTCTGCTTCTTTTTATAT+AGG + chr7_2:74274004-74274023 Msa1075860:intron 20.0%
!!! TTTCTGCTTCTTTTTATATA+GGG + chr7_2:74274005-74274024 Msa1075860:intron 20.0%
!! AACACAATTAAATACACAAA+AGG - chr7_2:74274222-74274241 None:intergenic 20.0%
!! AAATAACTGAACCATTATAA+TGG - chr7_2:74275620-74275639 None:intergenic 20.0%
!!! TATAATGGTTCAGTTATTTT+TGG + chr7_2:74275621-74275640 Msa1075860:intron 20.0%
!! ATTAAAAAGTGAATGCATAT+CGG + chr7_2:74276569-74276588 Msa1075860:intron 20.0%
!!! AAAGTTTTGATTTTAATTGC+AGG + chr7_2:74276988-74277007 Msa1075860:intron 20.0%
!!! AATTGTGTTATTTGAACAAA+TGG + chr7_2:74277332-74277351 Msa1075860:three_prime_UTR 20.0%
! ACAGTAAACAAGAACAAAAA+GGG - chr7_2:74273019-74273038 None:intergenic 25.0%
! AACAGTAAACAAGAACAAAA+AGG - chr7_2:74273020-74273039 None:intergenic 25.0%
!! ATTTTAGACTGAGATTGAAA+AGG - chr7_2:74273045-74273064 None:intergenic 25.0%
! ATTAGGGTTTAACAAAAATG+TGG - chr7_2:74273233-74273252 None:intergenic 25.0%
! GTTTAAAAACAGATCTATTG+TGG - chr7_2:74273525-74273544 None:intergenic 25.0%
!!! CAATAGATCTGTTTTTAAAC+TGG + chr7_2:74273525-74273544 Msa1075860:intron 25.0%
! CTGTCATATAGAAAATAACA+GGG - chr7_2:74273669-74273688 None:intergenic 25.0%
! ACTGTCATATAGAAAATAAC+AGG - chr7_2:74273670-74273689 None:intergenic 25.0%
! ATTACTTGTACAACATATCT+AGG + chr7_2:74273697-74273716 Msa1075860:intron 25.0%
! TTACTTGTACAACATATCTA+GGG + chr7_2:74273698-74273717 Msa1075860:intron 25.0%
! TTTATTTCATAAGCTTGTCA+AGG - chr7_2:74273858-74273877 None:intergenic 25.0%
! AAAAAATAAGCCAATCCAAT+TGG + chr7_2:74273914-74273933 Msa1075860:intron 25.0%
! AAAAATAAGCCAATCCAATT+GGG + chr7_2:74273915-74273934 Msa1075860:intron 25.0%
! ATTAAGAAAAAAGGGATTAG+GGG - chr7_2:74273941-74273960 None:intergenic 25.0%
!!! TCCCTTTTTTCTTAATTTAG+TGG + chr7_2:74273945-74273964 Msa1075860:intron 25.0%
! CCCACTAAATTAAGAAAAAA+GGG - chr7_2:74273949-74273968 None:intergenic 25.0%
!!! CCCTTTTTTCTTAATTTAGT+GGG + chr7_2:74273946-74273965 Msa1075860:intron 25.0%
!! TTCTGCTTCTTTTTATATAG+GGG + chr7_2:74274006-74274025 Msa1075860:intron 25.0%
!!! TCAAAATATTGGTTTTGGTT+CGG - chr7_2:74274177-74274196 None:intergenic 25.0%
! TACATTTCGGTTCAAAATAT+TGG - chr7_2:74274188-74274207 None:intergenic 25.0%
!! TTGCATTTTGGATAAAAAGA+AGG - chr7_2:74274252-74274271 None:intergenic 25.0%
! GCATGATATATTAGGAAAAA+TGG - chr7_2:74274406-74274425 None:intergenic 25.0%
! TGAATTCTGCATGATATATT+AGG - chr7_2:74274414-74274433 None:intergenic 25.0%
!!! GCAATATTTTGAATGTTCTA+TGG + chr7_2:74274493-74274512 Msa1075860:intron 25.0%
! TGAAAATTAGTGAGAAAGAA+AGG + chr7_2:74274636-74274655 Msa1075860:intron 25.0%
!! ATTAACATTTTCAAAGCCAT+CGG - chr7_2:74275061-74275080 None:intergenic 25.0%
!!! AATCATGGCTTTAATGAATA+TGG + chr7_2:74275139-74275158 Msa1075860:intron 25.0%
! TCACTGTAATACTATACTAA+AGG - chr7_2:74275165-74275184 None:intergenic 25.0%
! GTATATTGTGTGTTAAAATC+AGG - chr7_2:74275230-74275249 None:intergenic 25.0%
! CTGTTAATAAATTAACGGTT+CGG + chr7_2:74275586-74275605 Msa1075860:intron 25.0%
! CAATATATAAAAATAGGGGT+GGG - chr7_2:74275696-74275715 None:intergenic 25.0%
! TCAATATATAAAAATAGGGG+TGG - chr7_2:74275697-74275716 None:intergenic 25.0%
!!! TTTTGATCTTGCTTTAGAAT+AGG + chr7_2:74275776-74275795 Msa1075860:intron 25.0%
!!! TATTTTTCCTTGCTTTTCTT+TGG + chr7_2:74275801-74275820 Msa1075860:intron 25.0%
!! ATTAGTTTATCAAACATGCT+TGG - chr7_2:74276173-74276192 None:intergenic 25.0%
! AAGCATGTTTGATAAACTAA+TGG + chr7_2:74276172-74276191 Msa1075860:intron 25.0%
! TCTTCAATTGTAAGTTTAGA+AGG + chr7_2:74276347-74276366 Msa1075860:intron 25.0%
! TGAATCCTTGTATTCTAAAT+TGG + chr7_2:74276486-74276505 Msa1075860:intron 25.0%
!! TATTGCCAATTTAGAATACA+AGG - chr7_2:74276494-74276513 None:intergenic 25.0%
!! GTGCTTAATTTGATTGTATA+TGG - chr7_2:74276533-74276552 None:intergenic 25.0%
! TTTAATACAAGGAAATTCCT+CGG - chr7_2:74276555-74276574 None:intergenic 25.0%
!!! TGCATTCACTTTTTAATACA+AGG - chr7_2:74276566-74276585 None:intergenic 25.0%
! GATTGTTATCTATGTATTTC+TGG + chr7_2:74276960-74276979 Msa1075860:intron 25.0%
!!! GTTGACAAAGTTATTTTTCA+GGG + chr7_2:74277430-74277449 Msa1075860:three_prime_UTR 25.0%
!!! GAAAGAGTGAGGTTTTTTTT+GGG - chr7_2:74272919-74272938 None:intergenic 30.0%
!!! AGAAAGAGTGAGGTTTTTTT+TGG - chr7_2:74272920-74272939 None:intergenic 30.0%
AAGAATGGTACTATTGTTCA+TGG + chr7_2:74273130-74273149 Msa1075860:CDS 30.0%
!!! TTACATACGAAGCATTGTTT+TGG + chr7_2:74273494-74273513 Msa1075860:intron 30.0%
!!! TACATACGAAGCATTGTTTT+GGG + chr7_2:74273495-74273514 Msa1075860:intron 30.0%
ATTTGACATGAGCTGTTAAT+TGG + chr7_2:74273571-74273590 Msa1075860:intron 30.0%
AAGAATAAGTCAATCCAAAC+GGG - chr7_2:74273724-74273743 None:intergenic 30.0%
CAAGAATAAGTCAATCCAAA+CGG - chr7_2:74273725-74273744 None:intergenic 30.0%
GCCCACTAAATTAAGAAAAA+AGG - chr7_2:74273950-74273969 None:intergenic 30.0%
ACCATTTCAAAATTTCGGTT+CGG - chr7_2:74274064-74274083 None:intergenic 30.0%
!!! ACCGAAATTTTGAAATGGTT+CGG + chr7_2:74274065-74274084 Msa1075860:intron 30.0%
!!! TCGGTTCAAAATATTGGTTT+TGG - chr7_2:74274182-74274201 None:intergenic 30.0%
! GTAACCTTTTGAATGTTATG+TGG + chr7_2:74274462-74274481 Msa1075860:intron 30.0%
ATAAAATCGAGAAGAGTCAA+CGG - chr7_2:74274528-74274547 None:intergenic 30.0%
TCTATACGAAACAATCGAAA+AGG - chr7_2:74274614-74274633 None:intergenic 30.0%
GAGAAAGAAAGGAATTGTTT+CGG + chr7_2:74274647-74274666 Msa1075860:intron 30.0%
! GGGCAATGATTAAACTATTT+TGG - chr7_2:74274798-74274817 None:intergenic 30.0%
ATGCTTAGCTGAAAATTAAC+GGG - chr7_2:74274818-74274837 None:intergenic 30.0%
GATGCTTAGCTGAAAATTAA+CGG - chr7_2:74274819-74274838 None:intergenic 30.0%
ATAAAACATGAACGAAACCA+AGG - chr7_2:74274877-74274896 None:intergenic 30.0%
TTGCCATACTTATATTATCC+AGG + chr7_2:74274925-74274944 Msa1075860:intron 30.0%
TGTCCTGGATAATATAAGTA+TGG - chr7_2:74274931-74274950 None:intergenic 30.0%
!!! CTTGTCTTCGTTTTATTTCA+AGG + chr7_2:74274980-74274999 Msa1075860:intron 30.0%
AATGTTAATGCATAGAGAGA+AGG + chr7_2:74275072-74275091 Msa1075860:intron 30.0%
ATGTTAATGCATAGAGAGAA+GGG + chr7_2:74275073-74275092 Msa1075860:intron 30.0%
GCTTATTCAAAACAGCTTAA+CGG - chr7_2:74275310-74275329 None:intergenic 30.0%
!!! TGGTTCAGTTATTTTTGGTT+CGG + chr7_2:74275626-74275645 Msa1075860:intron 30.0%
AATATCACATTTACCATGCA+AGG - chr7_2:74275910-74275929 None:intergenic 30.0%
!!! TCCTTTGCCGTATATTTTTT+TGG + chr7_2:74276064-74276083 Msa1075860:intron 30.0%
ACCAAAAAAATATACGGCAA+AGG - chr7_2:74276068-74276087 None:intergenic 30.0%
AAGTTGGAAAGCTAAACTAA+TGG - chr7_2:74276264-74276283 None:intergenic 30.0%
! GAAAGTTAGGATTAGTAAGT+TGG - chr7_2:74276280-74276299 None:intergenic 30.0%
ACTTACTAATCCTAACTTTC+CGG + chr7_2:74276280-74276299 Msa1075860:intron 30.0%
ATTGAAGACCACATAATTCT+GGG - chr7_2:74276335-74276354 None:intergenic 30.0%
AATTGAAGACCACATAATTC+TGG - chr7_2:74276336-74276355 None:intergenic 30.0%
!! TTCTTGCCTTTTATTTGTGA+TGG + chr7_2:74276620-74276639 Msa1075860:intron 30.0%
!! TCTTGCCTTTTATTTGTGAT+GGG + chr7_2:74276621-74276640 Msa1075860:intron 30.0%
CTTCAACAAGTAAAGTTTCA+AGG - chr7_2:74276839-74276858 None:intergenic 30.0%
AACAATCCAACTAGACATAA+TGG - chr7_2:74276947-74276966 None:intergenic 30.0%
!!! GTTTTGATTTTAATTGCAGG+AGG + chr7_2:74276991-74277010 Msa1075860:intron 30.0%
ACCTGTGATACACAATTTAA+CGG - chr7_2:74277071-74277090 None:intergenic 30.0%
! ATTGTGTATCACAGGTTTTA+TGG + chr7_2:74277075-74277094 Msa1075860:three_prime_UTR 30.0%
TATGGATTTGACCTTATTGT+AGG + chr7_2:74277093-74277112 Msa1075860:three_prime_UTR 30.0%
ATGGATTTGACCTTATTGTA+GGG + chr7_2:74277094-74277113 Msa1075860:three_prime_UTR 30.0%
!!! CGTTGACAAAGTTATTTTTC+AGG + chr7_2:74277429-74277448 Msa1075860:three_prime_UTR 30.0%
CACTAAACACTAGTGTAGTT+CGG + chr7_2:74272891-74272910 Msa1075860:five_prime_UTR 35.0%
AAACAAGAACAAAAAGGGAG+AGG - chr7_2:74273014-74273033 None:intergenic 35.0%
ATTCTTCAACTCGATTGAGA+CGG - chr7_2:74273116-74273135 None:intergenic 35.0%
CAAAGCACAGAGCAAAATTA+GGG - chr7_2:74273205-74273224 None:intergenic 35.0%
! GAGAAACAGTTGACTTCAAT+TGG - chr7_2:74273326-74273345 None:intergenic 35.0%
GTCAAATGTCGAAATCTGAA+GGG - chr7_2:74273558-74273577 None:intergenic 35.0%
TGTCAAATGTCGAAATCTGA+AGG - chr7_2:74273559-74273578 None:intergenic 35.0%
! TGCTTCTTTTTATATAGGGG+TGG + chr7_2:74274009-74274028 Msa1075860:intron 35.0%
! GCTTCTTTTTATATAGGGGT+GGG + chr7_2:74274010-74274029 Msa1075860:intron 35.0%
!! TTCAAAATTTCGGTTCGGTT+CGG - chr7_2:74274059-74274078 None:intergenic 35.0%
!! ACCGAACCGAAATTTTGAAA+TGG + chr7_2:74274060-74274079 Msa1075860:intron 35.0%
CCCGAACCATTTCAAAATTT+CGG - chr7_2:74274069-74274088 None:intergenic 35.0%
!!! CCGAAATTTTGAAATGGTTC+GGG + chr7_2:74274066-74274085 Msa1075860:intron 35.0%
! ACCATAACTTTGGTTCAGTT+CGG - chr7_2:74274122-74274141 None:intergenic 35.0%
ACTGAACCAAAGTTATGGTT+CGG + chr7_2:74274123-74274142 Msa1075860:intron 35.0%
TCGGTTCATAGGTAAGAAAA+CGG - chr7_2:74274153-74274172 None:intergenic 35.0%
!!! ATATTGGTTTTGGTTCGGTT+CGG - chr7_2:74274172-74274191 None:intergenic 35.0%
CCATCCACATAACATTCAAA+AGG - chr7_2:74274469-74274488 None:intergenic 35.0%
! CCTTTTGAATGTTATGTGGA+TGG + chr7_2:74274466-74274485 Msa1075860:intron 35.0%
CTAGGAGGAGTTAAATGAAA+AGG - chr7_2:74274559-74274578 None:intergenic 35.0%
AAAGAAATGGACAAAGAGCT+AGG - chr7_2:74274577-74274596 None:intergenic 35.0%
ATCACACAACAAATCCTCAA+TGG - chr7_2:74274745-74274764 None:intergenic 35.0%
TGATTGACATAGTTACATCG+TGG + chr7_2:74274761-74274780 Msa1075860:intron 35.0%
GCCTCTATATATATGCAACA+AGG + chr7_2:74275009-74275028 Msa1075860:intron 35.0%
! GCCTTGTTGCATATATATAG+AGG - chr7_2:74275013-74275032 None:intergenic 35.0%
GCTTAACGGTTATTAGTTAG+TGG - chr7_2:74275296-74275315 None:intergenic 35.0%
AAACCACTTGAACTCTTTGT+TGG - chr7_2:74275530-74275549 None:intergenic 35.0%
AACAAAGAGTTCAAGTGGTT+TGG + chr7_2:74275529-74275548 Msa1075860:intron 35.0%
!!! CAGTTATTTTTGGTTCGGTT+CGG + chr7_2:74275631-74275650 Msa1075860:intron 35.0%
AAAATTTACCAGTAGACCCT+AGG + chr7_2:74275731-74275750 Msa1075860:intron 35.0%
ATCTAACAAGAAGCTACCTA+GGG - chr7_2:74275750-74275769 None:intergenic 35.0%
AATCTAACAAGAAGCTACCT+AGG - chr7_2:74275751-74275770 None:intergenic 35.0%
!! AGTTCTTGATTTGTCGTTTC+TGG + chr7_2:74275844-74275863 Msa1075860:intron 35.0%
TATTCTTGGTTATCCTTGCA+TGG + chr7_2:74275894-74275913 Msa1075860:intron 35.0%
AGAAAACAATGAGCTGACAA+CGG - chr7_2:74276039-74276058 None:intergenic 35.0%
GAAAGCTAAACTAATGGAAC+AGG - chr7_2:74276258-74276277 None:intergenic 35.0%
! ATAATTCTGGGGGATTCAAT+TGG - chr7_2:74276323-74276342 None:intergenic 35.0%
TTGAAGACCACATAATTCTG+GGG - chr7_2:74276334-74276353 None:intergenic 35.0%
! GTGAACATGATTTGTGAGTA+TGG + chr7_2:74276463-74276482 Msa1075860:intron 35.0%
! TACAATCAAATTAAGCACCG+AGG + chr7_2:74276535-74276554 Msa1075860:intron 35.0%
!! CTTGCCTTTTATTTGTGATG+GGG + chr7_2:74276622-74276641 Msa1075860:intron 35.0%
AACCGTTAAGCTAACTCTAA+AGG + chr7_2:74276734-74276753 Msa1075860:CDS 35.0%
ACCGTTAAGCTAACTCTAAA+GGG + chr7_2:74276735-74276754 Msa1075860:CDS 35.0%
CAGAAAATTGCCCTACAATA+AGG - chr7_2:74277107-74277126 None:intergenic 35.0%
! GTGTGTTATTGTTAGTTGTG+TGG + chr7_2:74277148-74277167 Msa1075860:three_prime_UTR 35.0%
! ATCTAGTTTATAATCCCTGC+TGG + chr7_2:74277213-74277232 Msa1075860:three_prime_UTR 35.0%
! TCTAGTTTATAATCCCTGCT+GGG + chr7_2:74277214-74277233 Msa1075860:three_prime_UTR 35.0%
AAGAACAAAAAGGGAGAGGA+AGG - chr7_2:74273010-74273029 None:intergenic 40.0%
TGAGAAATCTGCACGAGATT+TGG - chr7_2:74273075-74273094 None:intergenic 40.0%
!! GTCTCAATCGAGTTGAAGAA+TGG + chr7_2:74273115-74273134 Msa1075860:CDS 40.0%
ATTGTTCATGGCACTATCAC+AGG + chr7_2:74273142-74273161 Msa1075860:CDS 40.0%
GCAAAGCACAGAGCAAAATT+AGG - chr7_2:74273206-74273225 None:intergenic 40.0%
TCCATATCATGCCATTACAG+TGG + chr7_2:74273344-74273363 Msa1075860:intron 40.0%
CCATATCATGCCATTACAGT+GGG + chr7_2:74273345-74273364 Msa1075860:intron 40.0%
CATATCATGCCATTACAGTG+GGG + chr7_2:74273346-74273365 Msa1075860:intron 40.0%
ATCAAATCACCCCACTGTAA+TGG - chr7_2:74273358-74273377 None:intergenic 40.0%
ATTACAGTGGGGTGATTTGA+TGG + chr7_2:74273357-74273376 Msa1075860:intron 40.0%
! TACTAATTAGCTCTTCGCCT+TGG - chr7_2:74273637-74273656 None:intergenic 40.0%
CCAGACAGAACATCAACATT+TGG - chr7_2:74273972-74273991 None:intergenic 40.0%
CCAAATGTTGATGTTCTGTC+TGG + chr7_2:74273969-74273988 Msa1075860:intron 40.0%
! TTTTTATATAGGGGTGGGCA+TGG + chr7_2:74274015-74274034 Msa1075860:intron 40.0%
ACCGAACTGAACCAAAGTTA+TGG + chr7_2:74274118-74274137 Msa1075860:intron 40.0%
GGTTAACCGAACCATAACTT+TGG - chr7_2:74274132-74274151 None:intergenic 40.0%
GTATAGTGACTGTAATCCAC+TGG + chr7_2:74274719-74274738 Msa1075860:intron 40.0%
! GTAACACTGCAGAATTTTGC+AGG - chr7_2:74274843-74274862 None:intergenic 40.0%
AACATGAACGAAACCAAGGA+CGG - chr7_2:74274873-74274892 None:intergenic 40.0%
TATTCACATTGCGACACAGT+TGG + chr7_2:74274894-74274913 Msa1075860:intron 40.0%
AGCAAAGTTTCAAACTGTCC+TGG - chr7_2:74274946-74274965 None:intergenic 40.0%
GGTTTCAATTGCATGAAGGA+TGG - chr7_2:74275040-74275059 None:intergenic 40.0%
!! CATCGGTTTCAATTGCATGA+AGG - chr7_2:74275044-74275063 None:intergenic 40.0%
CTTCATGCAATTGAAACCGA+TGG + chr7_2:74275042-74275061 Msa1075860:intron 40.0%
GATCCAACAAAGAGTTCAAG+TGG + chr7_2:74275524-74275543 Msa1075860:intron 40.0%
!! AGAGTTCAAGTGGTTTGGTT+CGG + chr7_2:74275534-74275553 Msa1075860:intron 40.0%
CGGTTCGTGAACCATTATAA+TGG + chr7_2:74275606-74275625 Msa1075860:intron 40.0%
!!! ATTTTTGGTTCGGTTCGGTT+CGG + chr7_2:74275636-74275655 Msa1075860:intron 40.0%
CAGAACACCAAAGAAAAGCA+AGG - chr7_2:74275811-74275830 None:intergenic 40.0%
!!! TTTTCTTTGGTGTTCTGCTC+TGG + chr7_2:74275814-74275833 Msa1075860:intron 40.0%
!! TTTCTTTGGTGTTCTGCTCT+GGG + chr7_2:74275815-74275834 Msa1075860:intron 40.0%
TCTCCCCTTATCTGTACAAT+CGG + chr7_2:74275970-74275989 Msa1075860:intron 40.0%
TGTCCGATTGTACAGATAAG+GGG - chr7_2:74275976-74275995 None:intergenic 40.0%
CTGTCCGATTGTACAGATAA+GGG - chr7_2:74275977-74275996 None:intergenic 40.0%
ACTGTCCGATTGTACAGATA+AGG - chr7_2:74275978-74275997 None:intergenic 40.0%
TATCTGTACAATCGGACAGT+AGG + chr7_2:74275978-74275997 Msa1075860:intron 40.0%
TGACAACGGTTGAAGAGAAT+TGG - chr7_2:74276025-74276044 None:intergenic 40.0%
GCTGATGATATTGCAGCATA+TGG - chr7_2:74276127-74276146 None:intergenic 40.0%
ATGGAACAGGCATTAGAGAT+GGG - chr7_2:74276245-74276264 None:intergenic 40.0%
AATGGAACAGGCATTAGAGA+TGG - chr7_2:74276246-74276265 None:intergenic 40.0%
TTGAATCCCCCAGAATTATG+TGG + chr7_2:74276324-74276343 Msa1075860:intron 40.0%
TGAAGACCACATAATTCTGG+GGG - chr7_2:74276333-74276352 None:intergenic 40.0%
GTTTAGAAGGTCCAATGTGT+AGG + chr7_2:74276360-74276379 Msa1075860:intron 40.0%
TAGTAGACACACCTACACAT+TGG - chr7_2:74276374-74276393 None:intergenic 40.0%
CTCACCCCATCACAAATAAA+AGG - chr7_2:74276629-74276648 None:intergenic 40.0%
! GCATTAGTTGTACTGATCCT+TGG + chr7_2:74276661-74276680 Msa1075860:intron 40.0%
GTAACAAAGCAACAAGTCCA+AGG - chr7_2:74276681-74276700 None:intergenic 40.0%
!! TGGACTTGTTGCTTTGTTAC+AGG + chr7_2:74276681-74276700 Msa1075860:intron 40.0%
!! CCCCTTTAGAGTTAGCTTAA+CGG - chr7_2:74276739-74276758 None:intergenic 40.0%
! CCGTTAAGCTAACTCTAAAG+GGG + chr7_2:74276736-74276755 Msa1075860:CDS 40.0%
GGGGAAAAATCCAGTTACTT+TGG + chr7_2:74276755-74276774 Msa1075860:CDS 40.0%
CTGAGATGATCCAAAGTAAC+TGG - chr7_2:74276768-74276787 None:intergenic 40.0%
! TACTTGTTGAAGAAGCTCCT+AGG + chr7_2:74276847-74276866 Msa1075860:CDS 40.0%
! ACTTGTTGAAGAAGCTCCTA+GGG + chr7_2:74276848-74276867 Msa1075860:CDS 40.0%
TCAGAGCCATTATGTCTAGT+TGG + chr7_2:74276938-74276957 Msa1075860:intron 40.0%
GCCGTTAAATTGTGTATCAC+AGG + chr7_2:74277067-74277086 Msa1075860:exon 40.0%
GACAGCTTCTTTCAGTTGAT+TGG - chr7_2:74277252-74277271 None:intergenic 40.0%
CTGAAAGAAGCTGTCTTAGT+AGG + chr7_2:74277257-74277276 Msa1075860:three_prime_UTR 40.0%
CTGTCTTAGTAGGAGCTTAT+AGG + chr7_2:74277267-74277286 Msa1075860:three_prime_UTR 40.0%
AAATAGCGGCAGAAAGAGTG+AGG - chr7_2:74272930-74272949 None:intergenic 45.0%
CGGTGGAAAATCGCAAATAG+CGG - chr7_2:74272944-74272963 None:intergenic 45.0%
ATACCTAACGAGCTTCATGG+TGG - chr7_2:74272985-74273004 None:intergenic 45.0%
GTGATACCTAACGAGCTTCA+TGG - chr7_2:74272988-74273007 None:intergenic 45.0%
CCCACTGTAATGGCATGATA+TGG - chr7_2:74273348-74273367 None:intergenic 45.0%
GGGTGATTTGATGGAGTACA+CGG + chr7_2:74273366-74273385 Msa1075860:intron 45.0%
ATGGAGTACACGGTTGCTAT+TGG + chr7_2:74273376-74273395 Msa1075860:intron 45.0%
AACAACTTCCTGTCTTGCCA+AGG + chr7_2:74273617-74273636 Msa1075860:intron 45.0%
! GTTGATGTTCTGTCTGGAGT+TGG + chr7_2:74273975-74273994 Msa1075860:intron 45.0%
!!! TCGGTTCGGTTCGGTTTTTT+AGG - chr7_2:74274050-74274069 None:intergenic 45.0%
! AAACCGAACCGTTTCAGGTA+TGG + chr7_2:74274090-74274109 Msa1075860:intron 45.0%
! GGTTAGCTCCATACCTGAAA+CGG - chr7_2:74274101-74274120 None:intergenic 45.0%
!! TTTGGTTCGGTTCGGTTCAT+AGG - chr7_2:74274164-74274183 None:intergenic 45.0%
GAAATGGACAAAGAGCTAGG+AGG - chr7_2:74274574-74274593 None:intergenic 45.0%
AACAAATCCTCAATGGCCAG+TGG - chr7_2:74274738-74274757 None:intergenic 45.0%
ACATGAACGAAACCAAGGAC+GGG - chr7_2:74274872-74274891 None:intergenic 45.0%
!! TGGTTTGGTTCGGTTCAAGA+TGG + chr7_2:74275544-74275563 Msa1075860:intron 45.0%
!! TGGTTCGGTTCAAGATGGTT+CGG + chr7_2:74275549-74275568 Msa1075860:intron 45.0%
!! GGTTCAAGATGGTTCGGTTA+TGG + chr7_2:74275555-74275574 Msa1075860:intron 45.0%
TGGTGTGTTTCCGGAAAGTT+AGG - chr7_2:74276293-74276312 None:intergenic 45.0%
GGGATTCAATTGGAGGAGAA+TGG - chr7_2:74276313-74276332 None:intergenic 45.0%
! ATTCTGGGGGATTCAATTGG+AGG - chr7_2:74276320-74276339 None:intergenic 45.0%
! ATTTGTGATGGGGTGAGAAG+TGG + chr7_2:74276632-74276651 Msa1075860:intron 45.0%
TTGGATCATCTCAGTGTGAG+AGG + chr7_2:74276774-74276793 Msa1075860:CDS 45.0%
GTTTCAAGGTTCAAGCTGTC+AGG - chr7_2:74276825-74276844 None:intergenic 45.0%
TCGTATACAGTACCAGCAGT+TGG - chr7_2:74276888-74276907 None:intergenic 45.0%
AATTGCAGGAGGAAAGCCTT+TGG + chr7_2:74277002-74277021 Msa1075860:intron 45.0%
ATTGCAGGAGGAAAGCCTTT+GGG + chr7_2:74277003-74277022 Msa1075860:intron 45.0%
GCATTTGACATTACCCAGCA+GGG - chr7_2:74277230-74277249 None:intergenic 45.0%
TACCATTACCACTGCAGACA+AGG - chr7_2:74277313-74277332 None:intergenic 45.0%
GGTGGAGAAAAAGAGAGTCG+CGG - chr7_2:74272964-74272983 None:intergenic 50.0%
! CAACATATCTAGGGCCCGTT+TGG + chr7_2:74273707-74273726 Msa1075860:intron 50.0%
GATTAGGGGCCCAATTGGAT+TGG - chr7_2:74273927-74273946 None:intergenic 50.0%
AAAGGGATTAGGGGCCCAAT+TGG - chr7_2:74273932-74273951 None:intergenic 50.0%
ATATAGGGGTGGGCATGGTT+CGG + chr7_2:74274020-74274039 Msa1075860:intron 50.0%
GCTCCATACCTGAAACGGTT+CGG - chr7_2:74274096-74274115 None:intergenic 50.0%
CTGTAATCCACTGGCCATTG+AGG + chr7_2:74274728-74274747 Msa1075860:intron 50.0%
TGTTACGTTCTTCCCGTCCT+TGG + chr7_2:74274857-74274876 Msa1075860:intron 50.0%
AGAAGCTACCTAGGGTCTAC+TGG - chr7_2:74275742-74275761 None:intergenic 50.0%
CTGTACAATCGGACAGTAGG+AGG + chr7_2:74275981-74276000 Msa1075860:intron 50.0%
! CAGATGCACTGACGCTGTAT+AGG - chr7_2:74276209-74276228 None:intergenic 50.0%
GGAGGAGAATGGTGTGTTTC+CGG - chr7_2:74276302-74276321 None:intergenic 50.0%
TGATGGGGTGAGAAGTGGTT+TGG + chr7_2:74276637-74276656 Msa1075860:intron 50.0%
CAAGGTTCAAGCTGTCAGGA+AGG - chr7_2:74276821-74276840 None:intergenic 50.0%
AAGCCTAAGAAGCCAACTGC+TGG + chr7_2:74276873-74276892 Msa1075860:CDS 50.0%
! GTACCAGCAGTTGGCTTCTT+AGG - chr7_2:74276879-74276898 None:intergenic 50.0%
TTGCAGGAGGAAAGCCTTTG+GGG + chr7_2:74277004-74277023 Msa1075860:intron 50.0%
GGCATTTGACATTACCCAGC+AGG - chr7_2:74277231-74277250 None:intergenic 50.0%
TGCCTTGTCTGCAGTGGTAA+TGG + chr7_2:74277308-74277327 Msa1075860:three_prime_UTR 50.0%
GGAGAAAAAGAGAGTCGCGG+TGG - chr7_2:74272961-74272980 None:intergenic 55.0%
CCTAACGAGCTTCATGGTGG+TGG - chr7_2:74272982-74273001 None:intergenic 55.0%
CCACCACCATGAAGCTCGTT+AGG + chr7_2:74272979-74272998 Msa1075860:exon 55.0%
CGGGAAAACCGAACCGTTTC+AGG + chr7_2:74274085-74274104 Msa1075860:intron 55.0%
GGCTTCTTAGGCTTGACCCT+AGG - chr7_2:74276867-74276886 None:intergenic 55.0%
! CTGACTTGCCTTGTCTGCAG+TGG + chr7_2:74277302-74277321 Msa1075860:three_prime_UTR 55.0%
GCTCTTCGCCTTGGCAAGAC+AGG - chr7_2:74273628-74273647 None:intergenic 60.0%
!! GTTCGGTTCGGTTCGGTTCG+CGG + chr7_2:74275643-74275662 Msa1075860:intron 60.0%
!! TTCGGTTCGGTTCGGTTCGC+GGG + chr7_2:74275644-74275663 Msa1075860:intron 60.0%
!! TTCGGTTCGGTTCGCGGGTT+CGG + chr7_2:74275649-74275668 Msa1075860:intron 60.0%
! TTCGGTTCGCGGGTTCGGTT+CGG + chr7_2:74275654-74275673 Msa1075860:intron 60.0%
!! GAAAGCCTTTGGGGCGAGGA+CGG + chr7_2:74277013-74277032 Msa1075860:CDS 60.0%
!! AAAGCCTTTGGGGCGAGGAC+GGG + chr7_2:74277014-74277033 Msa1075860:CDS 60.0%
!! CGCGGGTTCGGTTCGGTTCA+TGG + chr7_2:74275661-74275680 Msa1075860:intron 65.0%
!! GGAGGAAAGCCTTTGGGGCG+AGG + chr7_2:74277009-74277028 Msa1075860:intron 65.0%
!! AAGCCTTTGGGGCGAGGACG+GGG + chr7_2:74277015-74277034 Msa1075860:CDS 65.0%
!! AGCCTTTGGGGCGAGGACGG+GGG + chr7_2:74277016-74277035 Msa1075860:CDS 70.0%
CGAGGACGTGGTCGTGGACG+CGG + chr7_2:74277039-74277058 Msa1075860:CDS 70.0%
CGTGGTCGTGGACGCGGTCG+TGG + chr7_2:74277045-74277064 Msa1075860:CDS 75.0%
! CGCCCCCGTCCTCGCCCCAA+AGG - chr7_2:74277021-74277040 None:intergenic 80.0%
! TTGGGGCGAGGACGGGGGCG+AGG + chr7_2:74277021-74277040 Msa1075860:CDS 80.0%
! CGAGGACGGGGGCGAGGACG+TGG + chr7_2:74277027-74277046 Msa1075860:CDS 80.0%
! CGGGGGCGAGGACGTGGTCG+TGG + chr7_2:74277033-74277052 Msa1075860:CDS 80.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 74272855 74277461 74272855 ID=Msa1075860;Name=Msa1075860
chr7_2 mRNA 74272855 74277461 74272855 ID=Msa1075860-mRNA-1;Parent=Msa1075860;Name=Msa1075860-mRNA-1;_AED=0.01;_eAED=0.01;_QI=132|1|1|1|0.66|0.5|4|387|115
chr7_2 exon 74272855 74273000 74272855 ID=Msa1075860-mRNA-1:exon:17380;Parent=Msa1075860-mRNA-1
chr7_2 exon 74273087 74273163 74273087 ID=Msa1075860-mRNA-1:exon:17381;Parent=Msa1075860-mRNA-1
chr7_2 exon 74276703 74276894 74276703 ID=Msa1075860-mRNA-1:exon:17382;Parent=Msa1075860-mRNA-1
chr7_2 exon 74277010 74277461 74277010 ID=Msa1075860-mRNA-1:exon:17383;Parent=Msa1075860-mRNA-1
chr7_2 five_prime_UTR 74272855 74272986 74272855 ID=Msa1075860-mRNA-1:five_prime_utr;Parent=Msa1075860-mRNA-1
chr7_2 CDS 74272987 74273000 74272987 ID=Msa1075860-mRNA-1:cds;Parent=Msa1075860-mRNA-1
chr7_2 CDS 74273087 74273163 74273087 ID=Msa1075860-mRNA-1:cds;Parent=Msa1075860-mRNA-1
chr7_2 CDS 74276703 74276894 74276703 ID=Msa1075860-mRNA-1:cds;Parent=Msa1075860-mRNA-1
chr7_2 CDS 74277010 74277074 74277010 ID=Msa1075860-mRNA-1:cds;Parent=Msa1075860-mRNA-1
chr7_2 three_prime_UTR 74277075 74277461 74277075 ID=Msa1075860-mRNA-1:three_prime_utr;Parent=Msa1075860-mRNA-1
Gene Sequence

>Msa1075860

ATGAAGCTCGTTAGATTTCTCATGAAGTTGAACAACGAAACCGTCTCAATCGAGTTGAAGAATGGTACTATTGTTCATGGCACTATCACAGGTGTTGACATCAGTATGAATACACATTTGAAAACCGTTAAGCTAACTCTAAAGGGGAAAAATCCAGTTACTTTGGATCATCTCAGTGTGAGAGGCAACAACATTCGTTATTATATCCTTCCTGACAGCTTGAACCTTGAAACTTTACTTGTTGAAGAAGCTCCTAGGGTCAAGCCTAAGAAGCCAACTGCTGGAGGAAAGCCTTTGGGGCGAGGACGGGGGCGAGGACGTGGTCGTGGACGCGGTCGTGGCCGTTAA
Protein sequence

>Msa1075860

MKLVRFLMKLNNETVSIELKNGTIVHGTITGVDISMNTHLKTVKLTLKGKNPVTLDHLSVRGNNIRYYILPDSLNLETLLVEEAPRVKPKKPTAGGKPLGRGRGRGRGRGRGRGR*