AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1117180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1117180 MtrunA17_Chr7g0259691 100.000 115 0 0 1 115 1 115 7.06e-78 224
Msa1117180 MtrunA17_Chr1g0185641 99.130 115 0 1 1 115 1 114 1.97e-75 218
Msa1117180 MtrunA17_Chr8g0383391 28.070 114 76 2 4 114 9 119 7.00e-12 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1117180 AT4G02840.1 92.857 98 7 0 1 98 1 98 1.87e-61 184
Msa1117180 AT3G07590.2 91.579 95 8 0 1 95 1 95 8.36e-60 179
Msa1117180 AT3G07590.1 91.579 95 8 0 1 95 1 95 8.36e-60 179
Msa1117180 AT4G02840.2 84.259 108 7 1 1 98 1 108 3.29e-58 176
Msa1117180 AT3G07590.3 87.879 66 8 0 30 95 4 69 4.57e-38 124
Msa1117180 AT1G20580.1 29.245 106 72 1 4 109 9 111 3.22e-12 59.3

Find 53 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCCTTTGGGGCGAGGACG+GGG 0.284771 7_3:+65466036 Msa1117180:CDS
GACAGCTTCTTTCAGTTGAT+TGG 0.291608 7_3:-65466270 None:intergenic
CTGTCTTAGTAGGAGCTTAT+AGG 0.302229 7_3:+65466288 Msa1117180:three_prime_UTR
GTACCAGCAGTTGGCTTCTT+AGG 0.317812 7_3:-65465897 None:intergenic
CCCCTTTAGAGTTAGCTTAA+CGG 0.355184 7_3:-65465757 None:intergenic
ACCGTTAAGCTAACTCTAAA+GGG 0.355730 7_3:+65465756 Msa1117180:CDS
CTTCAACAAGTAAAGTTTCA+AGG 0.362204 7_3:-65465857 None:intergenic
CTGAGATGATCCAAAGTAAC+TGG 0.363042 7_3:-65465786 None:intergenic
CAGAAAATTGCCCTACAATA+AGG 0.371910 7_3:-65466125 None:intergenic
TATGGATTTGACCTTATTGT+AGG 0.377788 7_3:+65466114 Msa1117180:three_prime_UTR
ATTGCAGGAGGAAAGCCTTT+GGG 0.380608 7_3:+65466024 Msa1117180:intron
ACCTGTGATACACAATTTAA+CGG 0.381233 7_3:-65466089 None:intergenic
AAAGCCTTTGGGGCGAGGAC+GGG 0.388156 7_3:+65466035 Msa1117180:CDS
AAGAATGGTACTATTGTTCA+TGG 0.396668 7_3:+65462464 Msa1117180:CDS
AATTGTGTTATTTGAACAAA+TGG 0.406190 7_3:+65466353 Msa1117180:three_prime_UTR
GTTTCAAGGTTCAAGCTGTC+AGG 0.435597 7_3:-65465843 None:intergenic
CTGAAAGAAGCTGTCTTAGT+AGG 0.440471 7_3:+65466278 Msa1117180:three_prime_UTR
AATTGCAGGAGGAAAGCCTT+TGG 0.442871 7_3:+65466023 Msa1117180:intron
GAAAGCCTTTGGGGCGAGGA+CGG 0.445123 7_3:+65466034 Msa1117180:CDS
CGTGGTCGTGGACGCGGTCG+TGG 0.448245 7_3:+65466066 Msa1117180:CDS
AACCGTTAAGCTAACTCTAA+AGG 0.451979 7_3:+65465755 Msa1117180:CDS
TGCCTTGTCTGCAGTGGTAA+TGG 0.454058 7_3:+65466329 Msa1117180:three_prime_UTR
CGAGGACGTGGTCGTGGACG+CGG 0.454065 7_3:+65466060 Msa1117180:CDS
GTGATACCTAACGAGCTTCA+TGG 0.454978 7_3:-65462319 None:intergenic
CCACCACCATGAAGCTCGTT+AGG 0.465256 7_3:+65462313 Msa1117180:exon
ATCTAGTTTATAATCCCTGC+TGG 0.466639 7_3:+65466234 Msa1117180:three_prime_UTR
GGCATTTGACATTACCCAGC+AGG 0.468140 7_3:-65466249 None:intergenic
GCCGTTAAATTGTGTATCAC+AGG 0.473452 7_3:+65466088 Msa1117180:exon
AGCCTTTGGGGCGAGGACGG+GGG 0.474600 7_3:+65466037 Msa1117180:CDS
GGAGGAAAGCCTTTGGGGCG+AGG 0.495880 7_3:+65466030 Msa1117180:intron
TACTTGTTGAAGAAGCTCCT+AGG 0.498295 7_3:+65465868 Msa1117180:CDS
ACTTGTTGAAGAAGCTCCTA+GGG 0.502700 7_3:+65465869 Msa1117180:CDS
CACTAAACACTAGTGTAGTT+CGG 0.503209 7_3:+65462225 Msa1117180:five_prime_UTR
ATGGATTTGACCTTATTGTA+GGG 0.504921 7_3:+65466115 Msa1117180:three_prime_UTR
CCTAACGAGCTTCATGGTGG+TGG 0.519786 7_3:-65462313 None:intergenic
GGCTTCTTAGGCTTGACCCT+AGG 0.525010 7_3:-65465885 None:intergenic
GTCTCAATCGAGTTGAAGAA+TGG 0.539433 7_3:+65462449 Msa1117180:CDS
ATTGTTCATGGCACTATCAC+AGG 0.543560 7_3:+65462476 Msa1117180:CDS
AAGCCTAAGAAGCCAACTGC+TGG 0.548844 7_3:+65465894 Msa1117180:CDS
GTGTGTTATTGTTAGTTGTG+TGG 0.568402 7_3:+65466169 Msa1117180:three_prime_UTR
TTGCAGGAGGAAAGCCTTTG+GGG 0.577723 7_3:+65466025 Msa1117180:intron
TACCATTACCACTGCAGACA+AGG 0.595627 7_3:-65466331 None:intergenic
GCATTTGACATTACCCAGCA+GGG 0.597689 7_3:-65466248 None:intergenic
ATTCTTCAACTCGATTGAGA+CGG 0.606708 7_3:-65462447 None:intergenic
TTGGATCATCTCAGTGTGAG+AGG 0.610982 7_3:+65465795 Msa1117180:CDS
CAAGGTTCAAGCTGTCAGGA+AGG 0.613711 7_3:-65465839 None:intergenic
TCTAGTTTATAATCCCTGCT+GGG 0.621220 7_3:+65466235 Msa1117180:three_prime_UTR
CCGTTAAGCTAACTCTAAAG+GGG 0.635466 7_3:+65465757 Msa1117180:CDS
CTGACTTGCCTTGTCTGCAG+TGG 0.640301 7_3:+65466323 Msa1117180:three_prime_UTR
TCGTATACAGTACCAGCAGT+TGG 0.655537 7_3:-65465906 None:intergenic
AAATAGCGGCAGAAAGAGTG+AGG 0.656474 7_3:-65462261 None:intergenic
CGGTGGAAAATCGCAAATAG+CGG 0.672058 7_3:-65462275 None:intergenic
ATACCTAACGAGCTTCATGG+TGG 0.754248 7_3:-65462316 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAATAATAATTA+GGG - chr7_3:65462583-65462602 None:intergenic 0.0%
!! TAATAATAATAATAATAATT+AGG - chr7_3:65462584-65462603 None:intergenic 0.0%
!!! TAAAAAAATATTTTGCATTT+TGG - chr7_3:65463265-65463284 None:intergenic 10.0%
!! AAAGTCAATATATAAAAATA+GGG - chr7_3:65464722-65464741 None:intergenic 10.0%
!! AAAAGTCAATATATAAAAAT+AGG - chr7_3:65464723-65464742 None:intergenic 10.0%
!! GGATAAAAAAAAATACATTT+CGG - chr7_3:65463202-65463221 None:intergenic 15.0%
!!! AGCAGTTTTTTTTTTAAATA+AGG - chr7_3:65463346-65463365 None:intergenic 15.0%
!! TACATAAATAAGAAAAGAAA+TGG - chr7_3:65463591-65463610 None:intergenic 15.0%
!! TGATATTAATTAATGAATCA+TGG + chr7_3:65464125-65464144 Msa1117180:intron 15.0%
!!! TGGTAAATATTTTAATCTTA+TGG - chr7_3:65464277-65464296 None:intergenic 15.0%
!!! ATAAGCTGTTTTTTAAAATA+AGG + chr7_3:65464325-65464344 Msa1117180:intron 15.0%
!!! ATTGAAAACAACTTATTTTA+TGG - chr7_3:65464357-65464376 None:intergenic 15.0%
!!! TAAGTTGTTTTCATAAAATA+AGG + chr7_3:65464443-65464462 Msa1117180:intron 15.0%
!!! TTTCAAATAAGTTGTTTTTT+TGG + chr7_3:65464501-65464520 Msa1117180:intron 15.0%
!! AAGAACTGTTAATAAATTAA+CGG + chr7_3:65464584-65464603 Msa1117180:intron 15.0%
!! AAGTCAATATATAAAAATAG+GGG - chr7_3:65464721-65464740 None:intergenic 15.0%
!! TTTAAATACTTTCTTATTCT+TGG + chr7_3:65464901-65464920 Msa1117180:intron 15.0%
!! AAGATTACCAAAAAAATATA+CGG - chr7_3:65465095-65465114 None:intergenic 15.0%
!!! TACAAATTATGTTTTGATTA+TGG + chr7_3:65465119-65465138 Msa1117180:intron 15.0%
!! ACAATTAGTATACAAAAATA+TGG - chr7_3:65465170-65465189 None:intergenic 15.0%
!! TATTATTACTACGAGTTTAA+TGG + chr7_3:65462596-65462615 Msa1117180:intron 20.0%
!!! TTTTCTGCTTCTTTTTATAT+AGG + chr7_3:65463000-65463019 Msa1117180:intron 20.0%
!!! TTTCTGCTTCTTTTTATATA+GGG + chr7_3:65463001-65463020 Msa1117180:intron 20.0%
!! AACACAATTAAATTCACAAA+AGG - chr7_3:65463223-65463242 None:intergenic 20.0%
!! AAATAACTGAACCATTATAA+TGG - chr7_3:65464623-65464642 None:intergenic 20.0%
!!! TATAATGGTTCAGTTATTTT+TGG + chr7_3:65464624-65464643 Msa1117180:intron 20.0%
!! ATTAAAAAGTGAATGCATAT+CGG + chr7_3:65465590-65465609 Msa1117180:intron 20.0%
!!! AAAGTTTTGATTTTAATTGC+AGG + chr7_3:65466009-65466028 Msa1117180:intron 20.0%
!!! AATTGTGTTATTTGAACAAA+TGG + chr7_3:65466353-65466372 Msa1117180:three_prime_UTR 20.0%
! ACAGTAAACAAGAACAAAAA+GGG - chr7_3:65462353-65462372 None:intergenic 25.0%
! AACAGTAAACAAGAACAAAA+AGG - chr7_3:65462354-65462373 None:intergenic 25.0%
!! ATTTTAGACTGAGATTGAAA+AGG - chr7_3:65462379-65462398 None:intergenic 25.0%
! AAATTAGGGTTTAAGAAAAG+CGG - chr7_3:65462525-65462544 None:intergenic 25.0%
! ATTAGGGTTTAACAAAAATG+TGG - chr7_3:65462567-65462586 None:intergenic 25.0%
! TTTATTTCATAAGCTTGTCA+AGG - chr7_3:65462842-65462861 None:intergenic 25.0%
! AAAAAATAAGCCAATCCAAT+TGG + chr7_3:65462898-65462917 Msa1117180:intron 25.0%
! AAAAATAAGCCAATCCAATT+GGG + chr7_3:65462899-65462918 Msa1117180:intron 25.0%
!!! TCCCTTTTTTCTTAATTTAG+TGG + chr7_3:65462941-65462960 Msa1117180:intron 25.0%
! CCCACTAAATTAAGAAAAAA+GGG - chr7_3:65462945-65462964 None:intergenic 25.0%
!!! CCCTTTTTTCTTAATTTAGT+GGG + chr7_3:65462942-65462961 Msa1117180:intron 25.0%
!! TTCTGCTTCTTTTTATATAG+GGG + chr7_3:65463002-65463021 Msa1117180:intron 25.0%
!!! TCAAAATATTGGTTTTGGTT+CGG - chr7_3:65463178-65463197 None:intergenic 25.0%
! TACATTTCGGTTCAAAATAT+TGG - chr7_3:65463189-65463208 None:intergenic 25.0%
!! TTGCATTTTGGATAAAAAGA+AGG - chr7_3:65463253-65463272 None:intergenic 25.0%
! GCATGATATATTAGGAAAAA+TGG - chr7_3:65463407-65463426 None:intergenic 25.0%
! TGAATTCTGCATGATATATT+AGG - chr7_3:65463415-65463434 None:intergenic 25.0%
!!! GCAATATTTTGAATGTTCTA+TGG + chr7_3:65463494-65463513 Msa1117180:intron 25.0%
! TGAAAATTAGTGAGAAAGAA+AGG + chr7_3:65463637-65463656 Msa1117180:intron 25.0%
!! ATTAACATTTTCAAAGCCAT+CGG - chr7_3:65464062-65464081 None:intergenic 25.0%
!!! AATCATGGCTTTAATGAATA+TGG + chr7_3:65464140-65464159 Msa1117180:intron 25.0%
! TCACTGTAATACTATACTAA+AGG - chr7_3:65464166-65464185 None:intergenic 25.0%
! GTATATTGTGTGTTAAAATC+AGG - chr7_3:65464231-65464250 None:intergenic 25.0%
! CTGTTAATAAATTAACGGTT+CGG + chr7_3:65464589-65464608 Msa1117180:intron 25.0%
! CAATATATAAAAATAGGGGT+GGG - chr7_3:65464717-65464736 None:intergenic 25.0%
! TCAATATATAAAAATAGGGG+TGG - chr7_3:65464718-65464737 None:intergenic 25.0%
!!! TTTTGATCTTGCTTTAGAAT+AGG + chr7_3:65464797-65464816 Msa1117180:intron 25.0%
!!! TATTTTTCCTTGCTTTTCTT+TGG + chr7_3:65464822-65464841 Msa1117180:intron 25.0%
!! ATTAGTTTATCAAACATGCT+TGG - chr7_3:65465194-65465213 None:intergenic 25.0%
! AAGCATGTTTGATAAACTAA+TGG + chr7_3:65465193-65465212 Msa1117180:intron 25.0%
! TCTTCAATTGTAAGTTTAGA+AGG + chr7_3:65465368-65465387 Msa1117180:intron 25.0%
! TGAATCCTTGTATTCTAAAT+TGG + chr7_3:65465507-65465526 Msa1117180:intron 25.0%
!! TATTGCCAATTTAGAATACA+AGG - chr7_3:65465515-65465534 None:intergenic 25.0%
!! GTGCTTAATTTGATTGTATA+TGG - chr7_3:65465554-65465573 None:intergenic 25.0%
! TTTAATACAAGGAAATTCCT+CGG - chr7_3:65465576-65465595 None:intergenic 25.0%
!!! TGCATTCACTTTTTAATACA+AGG - chr7_3:65465587-65465606 None:intergenic 25.0%
! GATTGTTATCTATGTATTTC+TGG + chr7_3:65465981-65466000 Msa1117180:intron 25.0%
!!! GTTGACAAAGTTATTTTTCA+GGG + chr7_3:65466451-65466470 Msa1117180:three_prime_UTR 25.0%
!!! GAAAGAGTGAGGTTTTTTTT+GGG - chr7_3:65462253-65462272 None:intergenic 30.0%
!!! AGAAAGAGTGAGGTTTTTTT+TGG - chr7_3:65462254-65462273 None:intergenic 30.0%
AAGAATGGTACTATTGTTCA+TGG + chr7_3:65462464-65462483 Msa1117180:CDS 30.0%
GCCCACTAAATTAAGAAAAA+AGG - chr7_3:65462946-65462965 None:intergenic 30.0%
ACCATTTCAAAATTTCGGTT+CGG - chr7_3:65463060-65463079 None:intergenic 30.0%
!!! ACCGAAATTTTGAAATGGTT+CGG + chr7_3:65463061-65463080 Msa1117180:intron 30.0%
!!! TCGGTTCAAAATATTGGTTT+TGG - chr7_3:65463183-65463202 None:intergenic 30.0%
! GTAACCTTTTGAATGTTATG+TGG + chr7_3:65463463-65463482 Msa1117180:intron 30.0%
ATAAAATCGAGAAGAGTCAA+CGG - chr7_3:65463529-65463548 None:intergenic 30.0%
TCTATACGAAACAATCGAAA+AGG - chr7_3:65463615-65463634 None:intergenic 30.0%
GAGAAAGAAAGGAATTGTTT+CGG + chr7_3:65463648-65463667 Msa1117180:intron 30.0%
! GGGCAATGATTAAACTATTT+TGG - chr7_3:65463799-65463818 None:intergenic 30.0%
ATGCTTAGCTGAAAATTAAC+GGG - chr7_3:65463819-65463838 None:intergenic 30.0%
GATGCTTAGCTGAAAATTAA+CGG - chr7_3:65463820-65463839 None:intergenic 30.0%
ATAAAACATGAACGAAACCA+AGG - chr7_3:65463878-65463897 None:intergenic 30.0%
TTGCCATACTTATATTATCC+AGG + chr7_3:65463926-65463945 Msa1117180:intron 30.0%
TGTCCTGGATAATATAAGTA+TGG - chr7_3:65463932-65463951 None:intergenic 30.0%
!!! CTTGTCTTCGTTTTATTTCA+AGG + chr7_3:65463981-65464000 Msa1117180:intron 30.0%
AATGTTAATGCATAGAGAGA+AGG + chr7_3:65464073-65464092 Msa1117180:intron 30.0%
ATGTTAATGCATAGAGAGAA+GGG + chr7_3:65464074-65464093 Msa1117180:intron 30.0%
GCTTATTCAAAACAGCTTAA+CGG - chr7_3:65464311-65464330 None:intergenic 30.0%
!!! TGGTTCAGTTATTTTTGGTT+CGG + chr7_3:65464629-65464648 Msa1117180:intron 30.0%
AATATCACATTTACCATGCA+AGG - chr7_3:65464931-65464950 None:intergenic 30.0%
!!! TCCTTTGCCGTATATTTTTT+TGG + chr7_3:65465085-65465104 Msa1117180:intron 30.0%
ACCAAAAAAATATACGGCAA+AGG - chr7_3:65465089-65465108 None:intergenic 30.0%
AAGTTGGAAAGCTAAACTAA+TGG - chr7_3:65465285-65465304 None:intergenic 30.0%
! GAAAGTTAGGATTAGTAAGT+TGG - chr7_3:65465301-65465320 None:intergenic 30.0%
ACTTACTAATCCTAACTTTC+CGG + chr7_3:65465301-65465320 Msa1117180:intron 30.0%
ATTGAAGACCACATAATTCT+GGG - chr7_3:65465356-65465375 None:intergenic 30.0%
AATTGAAGACCACATAATTC+TGG - chr7_3:65465357-65465376 None:intergenic 30.0%
!! TTCTTGCCTTTTATTTGTGA+TGG + chr7_3:65465641-65465660 Msa1117180:intron 30.0%
!! TCTTGCCTTTTATTTGTGAT+GGG + chr7_3:65465642-65465661 Msa1117180:intron 30.0%
CTTCAACAAGTAAAGTTTCA+AGG - chr7_3:65465860-65465879 None:intergenic 30.0%
AACAATCCAACTAGACATAA+TGG - chr7_3:65465968-65465987 None:intergenic 30.0%
!!! GTTTTGATTTTAATTGCAGG+AGG + chr7_3:65466012-65466031 Msa1117180:intron 30.0%
ACCTGTGATACACAATTTAA+CGG - chr7_3:65466092-65466111 None:intergenic 30.0%
! ATTGTGTATCACAGGTTTTA+TGG + chr7_3:65466096-65466115 Msa1117180:three_prime_UTR 30.0%
TATGGATTTGACCTTATTGT+AGG + chr7_3:65466114-65466133 Msa1117180:three_prime_UTR 30.0%
ATGGATTTGACCTTATTGTA+GGG + chr7_3:65466115-65466134 Msa1117180:three_prime_UTR 30.0%
!!! CGTTGACAAAGTTATTTTTC+AGG + chr7_3:65466450-65466469 Msa1117180:three_prime_UTR 30.0%
CACTAAACACTAGTGTAGTT+CGG + chr7_3:65462225-65462244 Msa1117180:five_prime_UTR 35.0%
AAACAAGAACAAAAAGGGAG+AGG - chr7_3:65462348-65462367 None:intergenic 35.0%
ATTCTTCAACTCGATTGAGA+CGG - chr7_3:65462450-65462469 None:intergenic 35.0%
CAAAGCACAGAGCAAAATTA+GGG - chr7_3:65462539-65462558 None:intergenic 35.0%
! GAGAAACAGTTGACTTCAAT+TGG - chr7_3:65462660-65462679 None:intergenic 35.0%
AGGGAATCCATATCCTATTA+GGG - chr7_3:65462926-65462945 None:intergenic 35.0%
AAGGGAATCCATATCCTATT+AGG - chr7_3:65462927-65462946 None:intergenic 35.0%
! TGCTTCTTTTTATATAGGGG+TGG + chr7_3:65463005-65463024 Msa1117180:intron 35.0%
! GCTTCTTTTTATATAGGGGT+GGG + chr7_3:65463006-65463025 Msa1117180:intron 35.0%
!! TTCAAAATTTCGGTTCGGTT+CGG - chr7_3:65463055-65463074 None:intergenic 35.0%
!! ACCGAACCGAAATTTTGAAA+TGG + chr7_3:65463056-65463075 Msa1117180:intron 35.0%
CCCGAACCATTTCAAAATTT+CGG - chr7_3:65463065-65463084 None:intergenic 35.0%
!!! CCGAAATTTTGAAATGGTTC+GGG + chr7_3:65463062-65463081 Msa1117180:intron 35.0%
! ACCATAACTTTGGTTCAGTT+CGG - chr7_3:65463123-65463142 None:intergenic 35.0%
ACTGAACCAAAGTTATGGTT+CGG + chr7_3:65463124-65463143 Msa1117180:intron 35.0%
TCGGTTCATAGGTAAGAAAA+CGG - chr7_3:65463154-65463173 None:intergenic 35.0%
!!! ATATTGGTTTTGGTTCGGTT+CGG - chr7_3:65463173-65463192 None:intergenic 35.0%
CCATCCACATAACATTCAAA+AGG - chr7_3:65463470-65463489 None:intergenic 35.0%
! CCTTTTGAATGTTATGTGGA+TGG + chr7_3:65463467-65463486 Msa1117180:intron 35.0%
CTAGGAGGAGTTAAATGAAA+AGG - chr7_3:65463560-65463579 None:intergenic 35.0%
AAAGAAATGGACAAAGAGCT+AGG - chr7_3:65463578-65463597 None:intergenic 35.0%
ATCACACAACAAATCCTCAA+TGG - chr7_3:65463746-65463765 None:intergenic 35.0%
TGATAGACATAGTTACATCG+TGG + chr7_3:65463762-65463781 Msa1117180:intron 35.0%
GCCTCTATATATATGCAACA+AGG + chr7_3:65464010-65464029 Msa1117180:intron 35.0%
! GCCTTGTTGCATATATATAG+AGG - chr7_3:65464014-65464033 None:intergenic 35.0%
GCTTAACGGTTATTAGTTAG+TGG - chr7_3:65464297-65464316 None:intergenic 35.0%
AAACCACTTGAACTCTTTGT+TGG - chr7_3:65464533-65464552 None:intergenic 35.0%
AACAAAGAGTTCAAGTGGTT+TGG + chr7_3:65464532-65464551 Msa1117180:intron 35.0%
!!! CAGTTATTTTTGGTTCGGTT+CGG + chr7_3:65464634-65464653 Msa1117180:intron 35.0%
AAAATTTACCAGTAGACCCT+AGG + chr7_3:65464752-65464771 Msa1117180:intron 35.0%
ATCTAACAAGAAGCTACCTA+GGG - chr7_3:65464771-65464790 None:intergenic 35.0%
AATCTAACAAGAAGCTACCT+AGG - chr7_3:65464772-65464791 None:intergenic 35.0%
!! AGTTCTTGATTTGTCGTTTC+TGG + chr7_3:65464865-65464884 Msa1117180:intron 35.0%
TATTCTTGGTTATCCTTGCA+TGG + chr7_3:65464915-65464934 Msa1117180:intron 35.0%
AGAAAACAATGAGCTGACAA+CGG - chr7_3:65465060-65465079 None:intergenic 35.0%
GAAAGCTAAACTAATGGAAC+AGG - chr7_3:65465279-65465298 None:intergenic 35.0%
! ATAATTCTGGGGGATTCAAT+TGG - chr7_3:65465344-65465363 None:intergenic 35.0%
TTGAAGACCACATAATTCTG+GGG - chr7_3:65465355-65465374 None:intergenic 35.0%
! GTGAACATGATTTGTGAGTA+TGG + chr7_3:65465484-65465503 Msa1117180:intron 35.0%
! TACAATCAAATTAAGCACCG+AGG + chr7_3:65465556-65465575 Msa1117180:intron 35.0%
!! CTTGCCTTTTATTTGTGATG+GGG + chr7_3:65465643-65465662 Msa1117180:intron 35.0%
AACCGTTAAGCTAACTCTAA+AGG + chr7_3:65465755-65465774 Msa1117180:CDS 35.0%
ACCGTTAAGCTAACTCTAAA+GGG + chr7_3:65465756-65465775 Msa1117180:CDS 35.0%
CAGAAAATTGCCCTACAATA+AGG - chr7_3:65466128-65466147 None:intergenic 35.0%
! GTGTGTTATTGTTAGTTGTG+TGG + chr7_3:65466169-65466188 Msa1117180:three_prime_UTR 35.0%
! ATCTAGTTTATAATCCCTGC+TGG + chr7_3:65466234-65466253 Msa1117180:three_prime_UTR 35.0%
! TCTAGTTTATAATCCCTGCT+GGG + chr7_3:65466235-65466254 Msa1117180:three_prime_UTR 35.0%
AAGAACAAAAAGGGAGAGGA+AGG - chr7_3:65462344-65462363 None:intergenic 40.0%
TGAGAAATCTGCACGAGATT+TGG - chr7_3:65462409-65462428 None:intergenic 40.0%
!! GTCTCAATCGAGTTGAAGAA+TGG + chr7_3:65462449-65462468 Msa1117180:CDS 40.0%
ATTGTTCATGGCACTATCAC+AGG + chr7_3:65462476-65462495 Msa1117180:CDS 40.0%
GCAAAGCACAGAGCAAAATT+AGG - chr7_3:65462540-65462559 None:intergenic 40.0%
TCCATATCATGCCATTACAG+TGG + chr7_3:65462678-65462697 Msa1117180:intron 40.0%
CCATATCATGCCATTACAGT+GGG + chr7_3:65462679-65462698 Msa1117180:intron 40.0%
CATATCATGCCATTACAGTG+GGG + chr7_3:65462680-65462699 Msa1117180:intron 40.0%
ATCAAATCACCCCACTGTAA+TGG - chr7_3:65462692-65462711 None:intergenic 40.0%
ATTACAGTGGGGTGATTTGA+TGG + chr7_3:65462691-65462710 Msa1117180:intron 40.0%
GGGAATCCATATCCTATTAG+GGG - chr7_3:65462925-65462944 None:intergenic 40.0%
CCAGACAGAACATCAACATT+TGG - chr7_3:65462968-65462987 None:intergenic 40.0%
CCAAATGTTGATGTTCTGTC+TGG + chr7_3:65462965-65462984 Msa1117180:intron 40.0%
! TTTTTATATAGGGGTGGGCA+TGG + chr7_3:65463011-65463030 Msa1117180:intron 40.0%
ACCGAACTGAACCAAAGTTA+TGG + chr7_3:65463119-65463138 Msa1117180:intron 40.0%
GGTTAACCGAACCATAACTT+TGG - chr7_3:65463133-65463152 None:intergenic 40.0%
GTATAGTGACTGTAATCCAC+TGG + chr7_3:65463720-65463739 Msa1117180:intron 40.0%
! GTAACACTGCAGAATTTTGC+AGG - chr7_3:65463844-65463863 None:intergenic 40.0%
AACATGAACGAAACCAAGGA+CGG - chr7_3:65463874-65463893 None:intergenic 40.0%
TATTCACATTGCGACACAGT+TGG + chr7_3:65463895-65463914 Msa1117180:intron 40.0%
AGCAAAGTTTCAAACTGTCC+TGG - chr7_3:65463947-65463966 None:intergenic 40.0%
GGTTTCAATTGCATGAAGGA+TGG - chr7_3:65464041-65464060 None:intergenic 40.0%
!! CATCGGTTTCAATTGCATGA+AGG - chr7_3:65464045-65464064 None:intergenic 40.0%
CTTCATGCAATTGAAACCGA+TGG + chr7_3:65464043-65464062 Msa1117180:intron 40.0%
GATCCAACAAAGAGTTCAAG+TGG + chr7_3:65464527-65464546 Msa1117180:intron 40.0%
!! AGAGTTCAAGTGGTTTGGTT+CGG + chr7_3:65464537-65464556 Msa1117180:intron 40.0%
CGGTTCGTGAACCATTATAA+TGG + chr7_3:65464609-65464628 Msa1117180:intron 40.0%
!!! ATTTTTGGTTCGGTTCGGTT+CGG + chr7_3:65464639-65464658 Msa1117180:intron 40.0%
CAGAACACCAAAGAAAAGCA+AGG - chr7_3:65464832-65464851 None:intergenic 40.0%
!!! TTTTCTTTGGTGTTCTGCTC+TGG + chr7_3:65464835-65464854 Msa1117180:intron 40.0%
!! TTTCTTTGGTGTTCTGCTCT+GGG + chr7_3:65464836-65464855 Msa1117180:intron 40.0%
TCTCCCCTTATCTGTACAAT+CGG + chr7_3:65464991-65465010 Msa1117180:intron 40.0%
TGTCCGATTGTACAGATAAG+GGG - chr7_3:65464997-65465016 None:intergenic 40.0%
CTGTCCGATTGTACAGATAA+GGG - chr7_3:65464998-65465017 None:intergenic 40.0%
ACTGTCCGATTGTACAGATA+AGG - chr7_3:65464999-65465018 None:intergenic 40.0%
TATCTGTACAATCGGACAGT+AGG + chr7_3:65464999-65465018 Msa1117180:intron 40.0%
TGACAACGGTTGAAGAGAAT+TGG - chr7_3:65465046-65465065 None:intergenic 40.0%
GCTGATGATATTGCAGCATA+TGG - chr7_3:65465148-65465167 None:intergenic 40.0%
ATGGAACAGGCATTAGAGAT+GGG - chr7_3:65465266-65465285 None:intergenic 40.0%
AATGGAACAGGCATTAGAGA+TGG - chr7_3:65465267-65465286 None:intergenic 40.0%
TTGAATCCCCCAGAATTATG+TGG + chr7_3:65465345-65465364 Msa1117180:intron 40.0%
TGAAGACCACATAATTCTGG+GGG - chr7_3:65465354-65465373 None:intergenic 40.0%
GTTTAGAAGGTCCAATGTGT+AGG + chr7_3:65465381-65465400 Msa1117180:intron 40.0%
TAGTAGACACACCTACACAT+TGG - chr7_3:65465395-65465414 None:intergenic 40.0%
CTCACCCCATCACAAATAAA+AGG - chr7_3:65465650-65465669 None:intergenic 40.0%
! GCATTAGTTGTACTGATCCT+TGG + chr7_3:65465682-65465701 Msa1117180:intron 40.0%
GTAACAAAGCAACAAGTCCA+AGG - chr7_3:65465702-65465721 None:intergenic 40.0%
!! TGGACTTGTTGCTTTGTTAC+AGG + chr7_3:65465702-65465721 Msa1117180:intron 40.0%
!! CCCCTTTAGAGTTAGCTTAA+CGG - chr7_3:65465760-65465779 None:intergenic 40.0%
! CCGTTAAGCTAACTCTAAAG+GGG + chr7_3:65465757-65465776 Msa1117180:CDS 40.0%
GGGGAAAAATCCAGTTACTT+TGG + chr7_3:65465776-65465795 Msa1117180:CDS 40.0%
CTGAGATGATCCAAAGTAAC+TGG - chr7_3:65465789-65465808 None:intergenic 40.0%
! TACTTGTTGAAGAAGCTCCT+AGG + chr7_3:65465868-65465887 Msa1117180:CDS 40.0%
! ACTTGTTGAAGAAGCTCCTA+GGG + chr7_3:65465869-65465888 Msa1117180:CDS 40.0%
TCAGAGCCATTATGTCTAGT+TGG + chr7_3:65465959-65465978 Msa1117180:intron 40.0%
GCCGTTAAATTGTGTATCAC+AGG + chr7_3:65466088-65466107 Msa1117180:exon 40.0%
GACAGCTTCTTTCAGTTGAT+TGG - chr7_3:65466273-65466292 None:intergenic 40.0%
CTGAAAGAAGCTGTCTTAGT+AGG + chr7_3:65466278-65466297 Msa1117180:three_prime_UTR 40.0%
CTGTCTTAGTAGGAGCTTAT+AGG + chr7_3:65466288-65466307 Msa1117180:three_prime_UTR 40.0%
AAATAGCGGCAGAAAGAGTG+AGG - chr7_3:65462264-65462283 None:intergenic 45.0%
CGGTGGAAAATCGCAAATAG+CGG - chr7_3:65462278-65462297 None:intergenic 45.0%
ATACCTAACGAGCTTCATGG+TGG - chr7_3:65462319-65462338 None:intergenic 45.0%
GTGATACCTAACGAGCTTCA+TGG - chr7_3:65462322-65462341 None:intergenic 45.0%
CCCACTGTAATGGCATGATA+TGG - chr7_3:65462682-65462701 None:intergenic 45.0%
TATTAGGGGCCCAATTGGAT+TGG - chr7_3:65462911-65462930 None:intergenic 45.0%
AATCCAATTGGGCCCCTAAT+AGG + chr7_3:65462910-65462929 Msa1117180:intron 45.0%
TATCCTATTAGGGGCCCAAT+TGG - chr7_3:65462916-65462935 None:intergenic 45.0%
ATTGGGCCCCTAATAGGATA+TGG + chr7_3:65462916-65462935 Msa1117180:intron 45.0%
! GTTGATGTTCTGTCTGGAGT+TGG + chr7_3:65462971-65462990 Msa1117180:intron 45.0%
!!! TCGGTTCGGTTCGGTTTTTT+AGG - chr7_3:65463046-65463065 None:intergenic 45.0%
!! ATACCTGAAACGGTTCGGTT+CGG - chr7_3:65463092-65463111 None:intergenic 45.0%
! GGTTAGCTCCATACCTGAAA+CGG - chr7_3:65463102-65463121 None:intergenic 45.0%
!! TTTGGTTCGGTTCGGTTCAT+AGG - chr7_3:65463165-65463184 None:intergenic 45.0%
GAAATGGACAAAGAGCTAGG+AGG - chr7_3:65463575-65463594 None:intergenic 45.0%
AACAAATCCTCAATGGCCAG+TGG - chr7_3:65463739-65463758 None:intergenic 45.0%
ACATGAACGAAACCAAGGAC+GGG - chr7_3:65463873-65463892 None:intergenic 45.0%
AGGTTCGTGATATATTCGCG+AGG - chr7_3:65464211-65464230 None:intergenic 45.0%
!! TGGTTTGGTTCGGTTCAAGA+TGG + chr7_3:65464547-65464566 Msa1117180:intron 45.0%
!! TGGTTCGGTTCAAGATGGTT+CGG + chr7_3:65464552-65464571 Msa1117180:intron 45.0%
!! GGTTCAAGATGGTTCGGTTA+TGG + chr7_3:65464558-65464577 Msa1117180:intron 45.0%
TGGTGTGTTTCCGGAAAGTT+AGG - chr7_3:65465314-65465333 None:intergenic 45.0%
GGGATTCAATTGGAGGAGAA+TGG - chr7_3:65465334-65465353 None:intergenic 45.0%
! ATTCTGGGGGATTCAATTGG+AGG - chr7_3:65465341-65465360 None:intergenic 45.0%
! ATTTGTGATGGGGTGAGAAG+TGG + chr7_3:65465653-65465672 Msa1117180:intron 45.0%
TTGGATCATCTCAGTGTGAG+AGG + chr7_3:65465795-65465814 Msa1117180:CDS 45.0%
GTTTCAAGGTTCAAGCTGTC+AGG - chr7_3:65465846-65465865 None:intergenic 45.0%
TCGTATACAGTACCAGCAGT+TGG - chr7_3:65465909-65465928 None:intergenic 45.0%
AATTGCAGGAGGAAAGCCTT+TGG + chr7_3:65466023-65466042 Msa1117180:intron 45.0%
ATTGCAGGAGGAAAGCCTTT+GGG + chr7_3:65466024-65466043 Msa1117180:intron 45.0%
GCATTTGACATTACCCAGCA+GGG - chr7_3:65466251-65466270 None:intergenic 45.0%
TACCATTACCACTGCAGACA+AGG - chr7_3:65466334-65466353 None:intergenic 45.0%
GGTGGAGAAAAAGAGAGTCG+CGG - chr7_3:65462298-65462317 None:intergenic 50.0%
ATATAGGGGTGGGCATGGTT+CGG + chr7_3:65463016-65463035 Msa1117180:intron 50.0%
AAACCGAACCGAACCGTTTC+AGG + chr7_3:65463086-65463105 Msa1117180:intron 50.0%
! GAACCGAACCGTTTCAGGTA+TGG + chr7_3:65463091-65463110 Msa1117180:intron 50.0%
GCTCCATACCTGAAACGGTT+CGG - chr7_3:65463097-65463116 None:intergenic 50.0%
CTGTAATCCACTGGCCATTG+AGG + chr7_3:65463729-65463748 Msa1117180:intron 50.0%
TGTTACGTTCTTCCCGTCCT+TGG + chr7_3:65463858-65463877 Msa1117180:intron 50.0%
AGAAGCTACCTAGGGTCTAC+TGG - chr7_3:65464763-65464782 None:intergenic 50.0%
CTGTACAATCGGACAGTAGG+AGG + chr7_3:65465002-65465021 Msa1117180:intron 50.0%
! CAGATGCACTGACGCTGTAT+AGG - chr7_3:65465230-65465249 None:intergenic 50.0%
GGAGGAGAATGGTGTGTTTC+CGG - chr7_3:65465323-65465342 None:intergenic 50.0%
TGATGGGGTGAGAAGTGGTT+TGG + chr7_3:65465658-65465677 Msa1117180:intron 50.0%
CAAGGTTCAAGCTGTCAGGA+AGG - chr7_3:65465842-65465861 None:intergenic 50.0%
AAGCCTAAGAAGCCAACTGC+TGG + chr7_3:65465894-65465913 Msa1117180:CDS 50.0%
! GTACCAGCAGTTGGCTTCTT+AGG - chr7_3:65465900-65465919 None:intergenic 50.0%
TTGCAGGAGGAAAGCCTTTG+GGG + chr7_3:65466025-65466044 Msa1117180:intron 50.0%
GGCATTTGACATTACCCAGC+AGG - chr7_3:65466252-65466271 None:intergenic 50.0%
TGCCTTGTCTGCAGTGGTAA+TGG + chr7_3:65466329-65466348 Msa1117180:three_prime_UTR 50.0%
GGAGAAAAAGAGAGTCGCGG+TGG - chr7_3:65462295-65462314 None:intergenic 55.0%
CCTAACGAGCTTCATGGTGG+TGG - chr7_3:65462316-65462335 None:intergenic 55.0%
CCACCACCATGAAGCTCGTT+AGG + chr7_3:65462313-65462332 Msa1117180:exon 55.0%
GGCTTCTTAGGCTTGACCCT+AGG - chr7_3:65465888-65465907 None:intergenic 55.0%
! CTGACTTGCCTTGTCTGCAG+TGG + chr7_3:65466323-65466342 Msa1117180:three_prime_UTR 55.0%
!! GTTCGGTTCGGTTCGGTTCG+CGG + chr7_3:65464646-65464665 Msa1117180:intron 60.0%
!! TTCGGTTCGGTTCGGTTCGC+GGG + chr7_3:65464647-65464666 Msa1117180:intron 60.0%
!! TTCGGTTCGGTTCGCGGGTT+CGG + chr7_3:65464652-65464671 Msa1117180:intron 60.0%
! TTCGGTTCGCGGGTTCGGTT+CGG + chr7_3:65464657-65464676 Msa1117180:intron 60.0%
!! TTCGGTTCGGTTCGCGGGTT+CGG + chr7_3:65464652-65464671 Msa1117180:intron 60.0%
! TTCGGTTCGCGGGTTCGGTT+CGG + chr7_3:65464657-65464676 Msa1117180:intron 60.0%
!! GAAAGCCTTTGGGGCGAGGA+CGG + chr7_3:65466034-65466053 Msa1117180:CDS 60.0%
!! AAAGCCTTTGGGGCGAGGAC+GGG + chr7_3:65466035-65466054 Msa1117180:CDS 60.0%
!! CGCGGGTTCGGTTCGGTTCA+TGG + chr7_3:65464682-65464701 Msa1117180:intron 65.0%
!! GGAGGAAAGCCTTTGGGGCG+AGG + chr7_3:65466030-65466049 Msa1117180:intron 65.0%
!! AAGCCTTTGGGGCGAGGACG+GGG + chr7_3:65466036-65466055 Msa1117180:CDS 65.0%
!! CGCGGGTTCGGTTCGGTTCG+CGG + chr7_3:65464664-65464683 Msa1117180:intron 70.0%
!! GCGGGTTCGGTTCGGTTCGC+GGG + chr7_3:65464665-65464684 Msa1117180:intron 70.0%
!! AGCCTTTGGGGCGAGGACGG+GGG + chr7_3:65466037-65466056 Msa1117180:CDS 70.0%
CGAGGACGTGGTCGTGGACG+CGG + chr7_3:65466060-65466079 Msa1117180:CDS 70.0%
CGTGGTCGTGGACGCGGTCG+TGG + chr7_3:65466066-65466085 Msa1117180:CDS 75.0%
! CGCCCCCGTCCTCGCCCCAA+AGG - chr7_3:65466042-65466061 None:intergenic 80.0%
! TTGGGGCGAGGACGGGGGCG+AGG + chr7_3:65466042-65466061 Msa1117180:CDS 80.0%
! CGAGGACGGGGGCGAGGACG+TGG + chr7_3:65466048-65466067 Msa1117180:CDS 80.0%
! CGGGGGCGAGGACGTGGTCG+TGG + chr7_3:65466054-65466073 Msa1117180:CDS 80.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 65462189 65466482 65462189 ID=Msa1117180;Name=Msa1117180
chr7_3 mRNA 65462189 65466482 65462189 ID=Msa1117180-mRNA-1;Parent=Msa1117180;Name=Msa1117180-mRNA-1;_AED=0.01;_eAED=0.01;_QI=132|1|1|1|0.66|0.5|4|387|115
chr7_3 exon 65462189 65462334 65462189 ID=Msa1117180-mRNA-1:exon:15539;Parent=Msa1117180-mRNA-1
chr7_3 exon 65462421 65462497 65462421 ID=Msa1117180-mRNA-1:exon:15540;Parent=Msa1117180-mRNA-1
chr7_3 exon 65465724 65465915 65465724 ID=Msa1117180-mRNA-1:exon:15541;Parent=Msa1117180-mRNA-1
chr7_3 exon 65466031 65466482 65466031 ID=Msa1117180-mRNA-1:exon:15542;Parent=Msa1117180-mRNA-1
chr7_3 five_prime_UTR 65462189 65462320 65462189 ID=Msa1117180-mRNA-1:five_prime_utr;Parent=Msa1117180-mRNA-1
chr7_3 CDS 65462321 65462334 65462321 ID=Msa1117180-mRNA-1:cds;Parent=Msa1117180-mRNA-1
chr7_3 CDS 65462421 65462497 65462421 ID=Msa1117180-mRNA-1:cds;Parent=Msa1117180-mRNA-1
chr7_3 CDS 65465724 65465915 65465724 ID=Msa1117180-mRNA-1:cds;Parent=Msa1117180-mRNA-1
chr7_3 CDS 65466031 65466095 65466031 ID=Msa1117180-mRNA-1:cds;Parent=Msa1117180-mRNA-1
chr7_3 three_prime_UTR 65466096 65466482 65466096 ID=Msa1117180-mRNA-1:three_prime_utr;Parent=Msa1117180-mRNA-1
Gene Sequence

>Msa1117180

ATGAAGCTCGTTAGATTTCTCATGAAGTTGAACAACGAAACCGTCTCAATCGAGTTGAAGAATGGTACTATTGTTCATGGCACTATCACAGGTGTTGACATCAGTATGAATACACATTTGAAAACCGTTAAGCTAACTCTAAAGGGGAAAAATCCAGTTACTTTGGATCATCTCAGTGTGAGAGGCAACAACATTCGTTATTATATCCTTCCTGACAGCTTGAACCTTGAAACTTTACTTGTTGAAGAAGCTCCTAGGGTCAAGCCTAAGAAGCCAACTGCTGGAGGAAAGCCTTTGGGGCGAGGACGGGGGCGAGGACGTGGTCGTGGACGCGGTCGTGGCCGTTAA
Protein sequence

>Msa1117180

MKLVRFLMKLNNETVSIELKNGTIVHGTITGVDISMNTHLKTVKLTLKGKNPVTLDHLSVRGNNIRYYILPDSLNLETLLVEEAPRVKPKKPTAGGKPLGRGRGRGRGRGRGRGR*