Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095420 | XP_013447886.1 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 3.55e-89 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095420 | A0A072TYJ6 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.70e-89 | 265 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1095420 | Msa1142580 | 0.829889 | 3.998070e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095420 | MtrunA17_Chr7g0223391 | 94.891 | 137 | 7 | 0 | 1 | 137 | 18 | 154 | 4.37e-93 | 265 |
| Msa1095420 | MtrunA17_Chr7g0223401 | 91.241 | 137 | 12 | 0 | 1 | 137 | 6 | 142 | 5.60e-89 | 254 |
| Msa1095420 | MtrunA17_Chr8g0356181 | 50.000 | 140 | 67 | 1 | 1 | 137 | 1 | 140 | 1.14e-42 | 137 |
| Msa1095420 | MtrunA17_Chr1g0190921 | 35.878 | 131 | 79 | 1 | 1 | 131 | 1 | 126 | 5.58e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095420 | AT2G44310.1 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 2.90e-40 | 132 |
Find 27 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_3:+18294926 | None:intergenic |
| TAAACACGAGCAAGCTCATT+TGG | 0.321898 | 7_3:-18295128 | None:intergenic |
| CATTCAAGCAGCCACTTTAC+CGG | 0.343259 | 7_3:-18295324 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_3:-18294971 | None:intergenic |
| TCTTCCAAGACCATTTGAAT+AGG | 0.402040 | 7_3:-18295266 | None:intergenic |
| CATGCCTATTCAAATGGTCT+TGG | 0.421269 | 7_3:+18295262 | Msa1095420:CDS |
| GGGTTTCATGCCTATTCAAA+TGG | 0.441768 | 7_3:+18295256 | Msa1095420:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_3:-18294965 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_3:-18295051 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_3:-18295127 | None:intergenic |
| TTAATAGCTCGATAGGAAAT+CGG | 0.457868 | 7_3:+18294991 | Msa1095420:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_3:+18295049 | Msa1095420:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_3:+18295167 | Msa1095420:CDS |
| GAAGCATTTAATAGCTCGAT+AGG | 0.503824 | 7_3:+18294984 | Msa1095420:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7_3:+18295235 | Msa1095420:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_3:+18295067 | Msa1095420:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7_3:+18295236 | Msa1095420:CDS |
| TGAAAGAGAGTCCGGTAAAG+TGG | 0.542820 | 7_3:+18295313 | Msa1095420:CDS |
| ATAGCCGTTGAAAGAGAGTC+CGG | 0.546530 | 7_3:+18295305 | Msa1095420:CDS |
| TTTACCGGACTCTCTTTCAA+CGG | 0.556793 | 7_3:-18295309 | None:intergenic |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_3:+18295066 | Msa1095420:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7_3:+18295230 | Msa1095420:CDS |
| GACCGTCGTTGTTTGTGTCG+AGG | 0.576811 | 7_3:-18295022 | None:intergenic |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_3:+18295097 | Msa1095420:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_3:-18294955 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_3:+18295223 | Msa1095420:CDS |
| TGCCTCGACACAAACAACGA+CGG | 0.702196 | 7_3:+18295020 | Msa1095420:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | + | chr7_3:18294930-18294949 | Msa1095420:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7_3:18294974-18294993 | None:intergenic | 30.0% | |
| TTAATAGCTCGATAGGAAAT+CGG | + | chr7_3:18294991-18295010 | Msa1095420:CDS | 30.0% | |
| ! | GAAGCATTTAATAGCTCGAT+AGG | + | chr7_3:18294984-18295003 | Msa1095420:CDS | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7_3:18295086-18295105 | Msa1095420:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7_3:18295097-18295116 | Msa1095420:CDS | 35.0% |
| TAAGCAAATGATGCTTGCAA+TGG | + | chr7_3:18295223-18295242 | Msa1095420:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+AGG | - | chr7_3:18295269-18295288 | None:intergenic | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7_3:18294958-18294977 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | - | chr7_3:18294968-18294987 | None:intergenic | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | + | chr7_3:18295049-18295068 | Msa1095420:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | - | chr7_3:18295054-18295073 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | - | chr7_3:18295130-18295149 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | - | chr7_3:18295131-18295150 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7_3:18295167-18295186 | Msa1095420:CDS | 40.0% | |
| GGGTTTCATGCCTATTCAAA+TGG | + | chr7_3:18295256-18295275 | Msa1095420:CDS | 40.0% | |
| CATGCCTATTCAAATGGTCT+TGG | + | chr7_3:18295262-18295281 | Msa1095420:CDS | 40.0% | |
| !! | CTCTTTCAACGGCTATTTTG+AGG | - | chr7_3:18295301-18295320 | None:intergenic | 40.0% |
| TTTACCGGACTCTCTTTCAA+CGG | - | chr7_3:18295312-18295331 | None:intergenic | 40.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7_3:18295230-18295249 | Msa1095420:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | + | chr7_3:18295236-18295255 | Msa1095420:CDS | 45.0% | |
| ATAGCCGTTGAAAGAGAGTC+CGG | + | chr7_3:18295305-18295324 | Msa1095420:CDS | 45.0% | |
| TGAAAGAGAGTCCGGTAAAG+TGG | + | chr7_3:18295313-18295332 | Msa1095420:CDS | 45.0% | |
| TGCCTCGACACAAACAACGA+CGG | + | chr7_3:18295020-18295039 | Msa1095420:CDS | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | + | chr7_3:18295066-18295085 | Msa1095420:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | + | chr7_3:18295067-18295086 | Msa1095420:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | + | chr7_3:18295235-18295254 | Msa1095420:CDS | 50.0% | |
| GACCGTCGTTGTTTGTGTCG+AGG | - | chr7_3:18295025-18295044 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 18294930 | 18295343 | 18294930 | ID=Msa1095420;Name=Msa1095420 |
| chr7_3 | mRNA | 18294930 | 18295343 | 18294930 | ID=Msa1095420-mRNA-1;Parent=Msa1095420;Name=Msa1095420-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_3 | exon | 18294930 | 18295343 | 18294930 | ID=Msa1095420-mRNA-1:exon:3969;Parent=Msa1095420-mRNA-1 |
| chr7_3 | CDS | 18294930 | 18295343 | 18294930 | ID=Msa1095420-mRNA-1:cds;Parent=Msa1095420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
