Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142580 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.90e-89 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142580 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 9.07e-90 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011800 | Msa1142580 | 0.824443 | 8.050710e-54 | -8.615850e-47 |
| Msa1053310 | Msa1142580 | 0.832778 | 7.786437e-56 | -8.615850e-47 |
| Msa1053340 | Msa1142580 | 0.839006 | 2.050859e-57 | -8.615850e-47 |
| Msa1095400 | Msa1142580 | 0.806746 | 7.080714e-50 | -8.615850e-47 |
| Msa1095410 | Msa1142580 | 0.809986 | 1.442393e-50 | -8.615850e-47 |
| Msa1095420 | Msa1142580 | 0.829889 | 3.998070e-55 | -8.615850e-47 |
| Msa1142570 | Msa1142580 | 0.820042 | 8.451714e-53 | -8.615850e-47 |
| Msa1142580 | Msa1142590 | 0.845259 | 4.540373e-59 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142580 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 3.04e-93 | 266 |
| Msa1142580 | MtrunA17_Chr7g0223401 | 91.971 | 137 | 11 | 0 | 1 | 137 | 6 | 142 | 3.53e-89 | 255 |
| Msa1142580 | MtrunA17_Chr8g0356181 | 49.286 | 140 | 68 | 1 | 1 | 137 | 1 | 140 | 1.99e-42 | 137 |
| Msa1142580 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 3.95e-20 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142580 | AT2G44310.1 | 47.445 | 137 | 70 | 1 | 1 | 135 | 1 | 137 | 1.85e-40 | 132 |
Find 28 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_4:-17099765 | None:intergenic |
| TAAACACGAGCAAGCTCATT+TGG | 0.321898 | 7_4:+17099563 | None:intergenic |
| CATTTAAGCAGCCACTTTAC+CGG | 0.324149 | 7_4:+17099367 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_4:+17099720 | None:intergenic |
| TCTTCCAAGACCATTTGAAT+AGG | 0.402040 | 7_4:+17099425 | None:intergenic |
| CATGCCTATTCAAATGGTCT+TGG | 0.421269 | 7_4:-17099429 | Msa1142580:CDS |
| GGGTTTCATGCCTATTCAAA+TGG | 0.441768 | 7_4:-17099435 | Msa1142580:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_4:+17099726 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7_4:-17099700 | Msa1142580:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_4:+17099640 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_4:+17099564 | None:intergenic |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_4:-17099642 | Msa1142580:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_4:-17099524 | Msa1142580:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7_4:-17099456 | Msa1142580:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_4:-17099624 | Msa1142580:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7_4:-17099455 | Msa1142580:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_4:-17099707 | Msa1142580:CDS |
| TGAAAGAGAGTCCGGTAAAG+TGG | 0.542820 | 7_4:-17099378 | Msa1142580:CDS |
| ATAGCCGTTGAAAGAGAGTC+CGG | 0.546530 | 7_4:-17099386 | Msa1142580:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_4:-17099711 | Msa1142580:CDS |
| TTTACCGGACTCTCTTTCAA+CGG | 0.556793 | 7_4:+17099382 | None:intergenic |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_4:-17099625 | Msa1142580:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7_4:-17099461 | Msa1142580:CDS |
| GACCGTCGTTGTTTGTGTCG+AGG | 0.576811 | 7_4:+17099669 | None:intergenic |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_4:-17099594 | Msa1142580:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_4:+17099736 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_4:-17099468 | Msa1142580:CDS |
| TGCCTCGACACAAACAACGA+CGG | 0.702196 | 7_4:-17099671 | Msa1142580:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_4:17099370-17099389 | Msa1142580:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_4:17099414-17099433 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | chr7_4:17099420-17099439 | Msa1142580:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | - | chr7_4:17099431-17099450 | Msa1142580:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_4:17099526-17099545 | Msa1142580:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_4:17099537-17099556 | Msa1142580:CDS | 35.0% |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_4:17099663-17099682 | Msa1142580:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+AGG | + | chr7_4:17099709-17099728 | None:intergenic | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_4:17099398-17099417 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_4:17099408-17099427 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | chr7_4:17099424-17099443 | Msa1142580:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_4:17099489-17099508 | Msa1142580:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_4:17099494-17099513 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_4:17099570-17099589 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_4:17099571-17099590 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_4:17099607-17099626 | Msa1142580:CDS | 40.0% | |
| GGGTTTCATGCCTATTCAAA+TGG | - | chr7_4:17099696-17099715 | Msa1142580:CDS | 40.0% | |
| CATGCCTATTCAAATGGTCT+TGG | - | chr7_4:17099702-17099721 | Msa1142580:CDS | 40.0% | |
| !! | CTCTTTCAACGGCTATTTTG+AGG | + | chr7_4:17099741-17099760 | None:intergenic | 40.0% |
| TTTACCGGACTCTCTTTCAA+CGG | + | chr7_4:17099752-17099771 | None:intergenic | 40.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_4:17099670-17099689 | Msa1142580:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | - | chr7_4:17099676-17099695 | Msa1142580:CDS | 45.0% | |
| ATAGCCGTTGAAAGAGAGTC+CGG | - | chr7_4:17099745-17099764 | Msa1142580:CDS | 45.0% | |
| TGAAAGAGAGTCCGGTAAAG+TGG | - | chr7_4:17099753-17099772 | Msa1142580:CDS | 45.0% | |
| TGCCTCGACACAAACAACGA+CGG | - | chr7_4:17099460-17099479 | Msa1142580:CDS | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | chr7_4:17099506-17099525 | Msa1142580:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | chr7_4:17099507-17099526 | Msa1142580:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | - | chr7_4:17099675-17099694 | Msa1142580:CDS | 50.0% | |
| GACCGTCGTTGTTTGTGTCG+AGG | + | chr7_4:17099465-17099484 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 17099370 | 17099783 | 17099370 | ID=Msa1142580;Name=Msa1142580 |
| chr7_4 | mRNA | 17099370 | 17099783 | 17099370 | ID=Msa1142580-mRNA-1;Parent=Msa1142580;Name=Msa1142580-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_4 | exon | 17099370 | 17099783 | 17099370 | ID=Msa1142580-mRNA-1:exon:3834;Parent=Msa1142580-mRNA-1 |
| chr7_4 | CDS | 17099370 | 17099783 | 17099370 | ID=Msa1142580-mRNA-1:cds;Parent=Msa1142580-mRNA-1 |
| Gene Sequence |
| Protein sequence |
