AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1100570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1100570 MtrunA17_Chr3g0145981 64.286 154 51 2 1 151 103 255 6.16e-66 204
Msa1100570 MtrunA17_Chr8g0370851 52.381 147 65 3 1 144 92 236 1.19e-50 164
Msa1100570 MtrunA17_Chr8g0370821 51.701 147 66 3 1 144 103 247 6.22e-49 160
Msa1100570 MtrunA17_Chr8g0370831 48.299 147 58 2 1 144 94 225 3.28e-42 142
Msa1100570 MtrunA17_Chr7g0270841 43.871 155 82 3 1 151 48 201 6.28e-40 136
Msa1100570 MtrunA17_Chr2g0329781 39.716 141 72 3 7 145 78 207 5.89e-31 112
Msa1100570 MtrunA17_Chr2g0329791 38.462 156 83 3 7 160 98 242 1.10e-30 112
Msa1100570 MtrunA17_Chr2g0294721 34.839 155 92 5 1 148 68 220 3.38e-23 93.2
Msa1100570 MtrunA17_Chr1g0206111 33.333 171 104 5 1 164 75 242 2.52e-22 90.9
Msa1100570 MtrunA17_Chr7g0221751 32.749 171 105 5 1 164 72 239 4.34e-22 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1100570 AT3G12500.1 60.390 154 57 2 1 151 98 250 6.72e-62 194
Msa1100570 AT4G01700.1 46.452 155 78 3 1 151 50 203 5.35e-44 147
Msa1100570 AT1G02360.1 42.581 155 84 3 1 151 42 195 9.99e-39 133
Msa1100570 AT1G02360.3 42.581 155 84 3 1 151 94 247 2.86e-38 133
Msa1100570 AT1G02360.2 42.581 155 84 3 1 151 88 241 3.32e-38 133
Msa1100570 AT3G54420.1 41.935 155 77 3 8 160 90 233 4.80e-35 123
Msa1100570 AT2G43590.1 37.267 161 84 4 2 160 80 225 1.47e-34 122
Msa1100570 AT2G43580.1 36.025 161 86 5 2 160 81 226 3.94e-31 113
Msa1100570 AT2G43610.1 37.107 159 84 3 2 160 100 242 2.66e-29 108
Msa1100570 AT2G43620.1 37.107 159 84 3 2 160 102 244 1.33e-28 107
Msa1100570 AT1G56680.1 42.282 149 69 5 2 149 96 228 1.14e-27 104
Msa1100570 AT1G56680.2 43.357 143 64 5 2 143 119 245 1.67e-27 104
Msa1100570 AT2G43600.1 38.255 149 74 4 2 149 92 223 4.82e-25 97.8
Msa1100570 AT2G43570.1 38.194 144 76 4 1 143 89 220 8.58e-25 97.1
Msa1100570 AT2G43600.2 38.255 149 74 4 2 149 113 244 9.37e-25 97.4
Msa1100570 AT1G05850.1 35.762 151 89 4 1 144 72 221 5.27e-23 93.2
Msa1100570 AT1G05850.2 35.762 151 89 4 1 144 72 221 5.27e-23 93.2
Msa1100570 AT3G16920.1 32.903 155 95 5 1 148 80 232 6.33e-21 87.8
Msa1100570 AT3G16920.2 32.903 155 95 5 1 148 104 256 8.87e-21 87.4
Msa1100570 AT3G47540.1 27.848 158 85 6 10 165 50 180 2.63e-12 62.8
Msa1100570 AT3G47540.2 27.848 158 85 6 10 165 50 180 4.93e-12 62.8

Find 57 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCCTTCGTTGCCGAACC+TGG 0.245191 7_3:-30587099 None:intergenic
TCAAGAGTGCTATATGGTTT+TGG 0.289569 7_3:+30587420 Msa1100570:CDS
TCTAAATTTCCATGTGTTCC+TGG 0.293914 7_3:+30587269 Msa1100570:CDS
ATCAAAGGGAATAAAATATA+TGG 0.311345 7_3:+30587323 Msa1100570:CDS
GGAGAATGTGGAAGTATAAT+AGG 0.323922 7_3:+30587344 Msa1100570:CDS
AAAGAAATATCCCCGGTCAT+AGG 0.328899 7_3:-30587201 None:intergenic
GCCGAACCTGGGGAATGTTT+TGG 0.339992 7_3:-30587087 None:intergenic
GGGTCTGTGGCAATGAGATC+TGG 0.352495 7_3:-30587386 None:intergenic
GCACTCTTGAAGGATATAAC+AGG 0.377927 7_3:-30587407 None:intergenic
AAGAAATATCCCCGGTCATA+GGG 0.408514 7_3:-30587200 None:intergenic
GGCTGGAAGGAATGCGCGAC+CGG 0.412108 7_3:+30587581 Msa1100570:CDS
GGAATGCGCGACCGGGTGGC+TGG 0.413441 7_3:+30587589 Msa1100570:CDS
TATCCTTCAAGAGTGCTATA+TGG 0.418078 7_3:+30587414 Msa1100570:CDS
AAACCATATAGCACTCTTGA+AGG 0.434167 7_3:-30587417 None:intergenic
TCTCCTTCGTTGCCGAACCT+GGG 0.454761 7_3:-30587098 None:intergenic
GCCAAAACATTCCCCAGGTT+CGG 0.455985 7_3:+30587086 Msa1100570:CDS
CTAAATTTCCATGTGTTCCT+GGG 0.462737 7_3:+30587270 Msa1100570:CDS
ACACATGGAAATTTAGAGCT+TGG 0.463623 7_3:-30587263 None:intergenic
CACCTCTTAGCCCCTATGAC+CGG 0.464103 7_3:+30587189 Msa1100570:CDS
TTCGCAGTAGTCTTCTTCGA+TGG 0.468797 7_3:-30587240 None:intergenic
CTCTCGTAGTATTGCTTCCC+AGG 0.474080 7_3:-30587287 None:intergenic
ACCTCTTAGCCCCTATGACC+GGG 0.474353 7_3:+30587190 Msa1100570:CDS
CAAGATGGCTTTACTTGGAA+AGG 0.479312 7_3:-30587464 None:intergenic
TCGCAGTAGTCTTCTTCGAT+GGG 0.491948 7_3:-30587239 None:intergenic
CCCCGGTCATAGGGGCTAAG+AGG 0.498386 7_3:-30587191 None:intergenic
AAGATGGCTTTACTTGGAAA+GGG 0.503243 7_3:-30587463 None:intergenic
TTATTCCCTTTGATCTGAAG+CGG 0.516176 7_3:-30587314 None:intergenic
AATAAAATATATGGAGAATG+TGG 0.517331 7_3:+30587332 Msa1100570:CDS
AGGGGTCCGCTTCAGATCAA+AGG 0.525246 7_3:+30587308 Msa1100570:CDS
GGCTTTACTTGGAAAGGGAA+AGG 0.540330 7_3:-30587458 None:intergenic
GGTCTGTGGCAATGAGATCT+GGG 0.547304 7_3:-30587385 None:intergenic
CCACATATGTCATAGAAGGC+TGG 0.554306 7_3:+30587564 Msa1100570:intron
ACTTGGAAAGGGAAAGGTGG+AGG 0.555116 7_3:-30587452 None:intergenic
GGGGTCCGCTTCAGATCAAA+GGG 0.557701 7_3:+30587309 Msa1100570:CDS
AAGCTGCCAAAACATTCCCC+AGG 0.566465 7_3:+30587081 Msa1100570:CDS
CTCGTTTACATCCAGCCACC+CGG 0.571779 7_3:-30587600 None:intergenic
GATGATGAGTGTGAGGGGAA+AGG 0.572756 7_3:+30587032 Msa1100570:CDS
GCTGGAAGGAATGCGCGACC+GGG 0.573064 7_3:+30587582 Msa1100570:CDS
AGCTCTCACAAAACAAGAGG+TGG 0.574719 7_3:+30587164 Msa1100570:CDS
GGAAGGAATGCGCGACCGGG+TGG 0.575460 7_3:+30587585 Msa1100570:CDS
CTCCTTCGTTGCCGAACCTG+GGG 0.576604 7_3:-30587097 None:intergenic
TATTGCTTCCCAGGAACACA+TGG 0.580157 7_3:-30587278 None:intergenic
TTCCCCAGGTTCGGCAACGA+AGG 0.585501 7_3:+30587095 Msa1100570:CDS
CACTCTTGAAGGATATAACA+GGG 0.603547 7_3:-30587406 None:intergenic
CGCGACGATGATGAGTGTGA+GGG 0.608511 7_3:+30587026 Msa1100570:CDS
CATTCACAAAGAAATATCCC+CGG 0.613688 7_3:-30587208 None:intergenic
TCGCGACGATGATGAGTGTG+AGG 0.615130 7_3:+30587025 Msa1100570:CDS
AGAAATATCCCCGGTCATAG+GGG 0.616770 7_3:-30587199 None:intergenic
GAAGGATATAACAGGGTCTG+TGG 0.619235 7_3:-30587399 None:intergenic
CCTCTTAGCCCCTATGACCG+GGG 0.619811 7_3:+30587191 Msa1100570:CDS
CTGGGAAGCAATACTACGAG+AGG 0.622203 7_3:+30587288 Msa1100570:CDS
CAAAGCTCTCACAAAACAAG+AGG 0.630156 7_3:+30587161 Msa1100570:CDS
ATATGTCATAGAAGGCTGGA+AGG 0.630999 7_3:+30587568 Msa1100570:intron
TGGGAAGCAATACTACGAGA+GGG 0.631128 7_3:+30587289 Msa1100570:CDS
TTTACTTGGAAAGGGAAAGG+TGG 0.645661 7_3:-30587455 None:intergenic
GCGACGATGATGAGTGTGAG+GGG 0.672599 7_3:+30587027 Msa1100570:CDS
GGGAAGCAATACTACGAGAG+GGG 0.685899 7_3:+30587290 Msa1100570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAAGGGAATAAAATATA+TGG + chr7_3:30587323-30587342 Msa1100570:CDS 20.0%
!! AATAAAATATATGGAGAATG+TGG + chr7_3:30587332-30587351 Msa1100570:CDS 20.0%
! GACTAAAATCAAAATCAGTA+GGG - chr7_3:30587517-30587536 None:intergenic 25.0%
! TGACTAAAATCAAAATCAGT+AGG - chr7_3:30587518-30587537 None:intergenic 25.0%
!!! AGAGAAATTGCTGCTTTTTT+TGG + chr7_3:30587137-30587156 Msa1100570:CDS 30.0%
!!! CTGATTTTGATTTTAGTCAG+AGG + chr7_3:30587519-30587538 Msa1100570:intron 30.0%
!!! ATTTTGATTTTAGTCAGAGG+AGG + chr7_3:30587522-30587541 Msa1100570:intron 30.0%
CATTCACAAAGAAATATCCC+CGG - chr7_3:30587211-30587230 None:intergenic 35.0%
! ACACATGGAAATTTAGAGCT+TGG - chr7_3:30587266-30587285 None:intergenic 35.0%
TCTAAATTTCCATGTGTTCC+TGG + chr7_3:30587269-30587288 Msa1100570:CDS 35.0%
CTAAATTTCCATGTGTTCCT+GGG + chr7_3:30587270-30587289 Msa1100570:CDS 35.0%
TTATTCCCTTTGATCTGAAG+CGG - chr7_3:30587317-30587336 None:intergenic 35.0%
! GGAGAATGTGGAAGTATAAT+AGG + chr7_3:30587344-30587363 Msa1100570:CDS 35.0%
CACTCTTGAAGGATATAACA+GGG - chr7_3:30587409-30587428 None:intergenic 35.0%
!! TATCCTTCAAGAGTGCTATA+TGG + chr7_3:30587414-30587433 Msa1100570:CDS 35.0%
AAACCATATAGCACTCTTGA+AGG - chr7_3:30587420-30587439 None:intergenic 35.0%
!!! TCAAGAGTGCTATATGGTTT+TGG + chr7_3:30587420-30587439 Msa1100570:CDS 35.0%
! AAGATGGCTTTACTTGGAAA+GGG - chr7_3:30587466-30587485 None:intergenic 35.0%
ATGACCACATATGTCATAGA+AGG + chr7_3:30587560-30587579 Msa1100570:intron 35.0%
CAAAGCTCTCACAAAACAAG+AGG + chr7_3:30587161-30587180 Msa1100570:CDS 40.0%
AAGAAATATCCCCGGTCATA+GGG - chr7_3:30587203-30587222 None:intergenic 40.0%
AAAGAAATATCCCCGGTCAT+AGG - chr7_3:30587204-30587223 None:intergenic 40.0%
GCACTCTTGAAGGATATAAC+AGG - chr7_3:30587410-30587429 None:intergenic 40.0%
TTTACTTGGAAAGGGAAAGG+TGG - chr7_3:30587458-30587477 None:intergenic 40.0%
! CAAGATGGCTTTACTTGGAA+AGG - chr7_3:30587467-30587486 None:intergenic 40.0%
ATATGTCATAGAAGGCTGGA+AGG + chr7_3:30587568-30587587 Msa1100570:intron 40.0%
AGCTCTCACAAAACAAGAGG+TGG + chr7_3:30587164-30587183 Msa1100570:CDS 45.0%
AGAAATATCCCCGGTCATAG+GGG - chr7_3:30587202-30587221 None:intergenic 45.0%
TCGCAGTAGTCTTCTTCGAT+GGG - chr7_3:30587242-30587261 None:intergenic 45.0%
TTCGCAGTAGTCTTCTTCGA+TGG - chr7_3:30587243-30587262 None:intergenic 45.0%
TATTGCTTCCCAGGAACACA+TGG - chr7_3:30587281-30587300 None:intergenic 45.0%
TGGGAAGCAATACTACGAGA+GGG + chr7_3:30587289-30587308 Msa1100570:CDS 45.0%
GAAGGATATAACAGGGTCTG+TGG - chr7_3:30587402-30587421 None:intergenic 45.0%
GGCTTTACTTGGAAAGGGAA+AGG - chr7_3:30587461-30587480 None:intergenic 45.0%
TTTAGTCAGAGGAGGTTTCC+AGG + chr7_3:30587530-30587549 Msa1100570:intron 45.0%
AGAGGAGGTTTCCAGGATAT+GGG + chr7_3:30587537-30587556 Msa1100570:intron 45.0%
TATGTGGTCATCCCATATCC+TGG - chr7_3:30587551-30587570 None:intergenic 45.0%
CCAGCCTTCTATGACATATG+TGG - chr7_3:30587567-30587586 None:intergenic 45.0%
CCACATATGTCATAGAAGGC+TGG + chr7_3:30587564-30587583 Msa1100570:intron 45.0%
GATGATGAGTGTGAGGGGAA+AGG + chr7_3:30587032-30587051 Msa1100570:CDS 50.0%
AAGCTGCCAAAACATTCCCC+AGG + chr7_3:30587081-30587100 Msa1100570:CDS 50.0%
GCCAAAACATTCCCCAGGTT+CGG + chr7_3:30587086-30587105 Msa1100570:CDS 50.0%
CTCTCGTAGTATTGCTTCCC+AGG - chr7_3:30587290-30587309 None:intergenic 50.0%
CTGGGAAGCAATACTACGAG+AGG + chr7_3:30587288-30587307 Msa1100570:CDS 50.0%
GGGAAGCAATACTACGAGAG+GGG + chr7_3:30587290-30587309 Msa1100570:CDS 50.0%
GGTCTGTGGCAATGAGATCT+GGG - chr7_3:30587388-30587407 None:intergenic 50.0%
ACTTGGAAAGGGAAAGGTGG+AGG - chr7_3:30587455-30587474 None:intergenic 50.0%
! CGTGGCAAGATGGCTTTACT+TGG - chr7_3:30587472-30587491 None:intergenic 50.0%
GTTATAGCGTCGTGGCAAGA+TGG - chr7_3:30587482-30587501 None:intergenic 50.0%
TCTTGCCACGACGCTATAAC+CGG + chr7_3:30587482-30587501 Msa1100570:intron 50.0%
ATCAGTAGGGATCCATCGTC+CGG - chr7_3:30587504-30587523 None:intergenic 50.0%
CAGAGGAGGTTTCCAGGATA+TGG + chr7_3:30587536-30587555 Msa1100570:intron 50.0%
TCGCGACGATGATGAGTGTG+AGG + chr7_3:30587025-30587044 Msa1100570:CDS 55.0%
CGCGACGATGATGAGTGTGA+GGG + chr7_3:30587026-30587045 Msa1100570:CDS 55.0%
GCGACGATGATGAGTGTGAG+GGG + chr7_3:30587027-30587046 Msa1100570:CDS 55.0%
! GCCGAACCTGGGGAATGTTT+TGG - chr7_3:30587090-30587109 None:intergenic 55.0%
TCTCCTTCGTTGCCGAACCT+GGG - chr7_3:30587101-30587120 None:intergenic 55.0%
CACCTCTTAGCCCCTATGAC+CGG + chr7_3:30587189-30587208 Msa1100570:CDS 55.0%
ACCTCTTAGCCCCTATGACC+GGG + chr7_3:30587190-30587209 Msa1100570:CDS 55.0%
AGGGGTCCGCTTCAGATCAA+AGG + chr7_3:30587308-30587327 Msa1100570:CDS 55.0%
GGGGTCCGCTTCAGATCAAA+GGG + chr7_3:30587309-30587328 Msa1100570:CDS 55.0%
GGGTCTGTGGCAATGAGATC+TGG - chr7_3:30587389-30587408 None:intergenic 55.0%
ATCGTCCGGTTATAGCGTCG+TGG - chr7_3:30587490-30587509 None:intergenic 55.0%
ACGACGCTATAACCGGACGA+TGG + chr7_3:30587489-30587508 Msa1100570:intron 55.0%
TTCCCCAGGTTCGGCAACGA+AGG + chr7_3:30587095-30587114 Msa1100570:CDS 60.0%
CTCCTTCGTTGCCGAACCTG+GGG - chr7_3:30587100-30587119 None:intergenic 60.0%
GTCTCCTTCGTTGCCGAACC+TGG - chr7_3:30587102-30587121 None:intergenic 60.0%
CCTCTTAGCCCCTATGACCG+GGG + chr7_3:30587191-30587210 Msa1100570:CDS 60.0%
CCCCGGTCATAGGGGCTAAG+AGG - chr7_3:30587194-30587213 None:intergenic 65.0%
GGCTGGAAGGAATGCGCGAC+CGG + chr7_3:30587581-30587600 Msa1100570:CDS 65.0%
GCTGGAAGGAATGCGCGACC+GGG + chr7_3:30587582-30587601 Msa1100570:CDS 65.0%
GGAAGGAATGCGCGACCGGG+TGG + chr7_3:30587585-30587604 Msa1100570:CDS 70.0%
GGAATGCGCGACCGGGTGGC+TGG + chr7_3:30587589-30587608 Msa1100570:CDS 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 30587014 30587617 30587014 ID=Msa1100570;Name=Msa1100570
chr7_3 mRNA 30587014 30587617 30587014 ID=Msa1100570-mRNA-1;Parent=Msa1100570;Name=Msa1100570-mRNA-1;_AED=0.37;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|165
chr7_3 exon 30587014 30587472 30587014 ID=Msa1100570-mRNA-1:exon:6483;Parent=Msa1100570-mRNA-1
chr7_3 exon 30587579 30587617 30587579 ID=Msa1100570-mRNA-1:exon:6484;Parent=Msa1100570-mRNA-1
chr7_3 CDS 30587014 30587472 30587014 ID=Msa1100570-mRNA-1:cds;Parent=Msa1100570-mRNA-1
chr7_3 CDS 30587579 30587617 30587579 ID=Msa1100570-mRNA-1:cds;Parent=Msa1100570-mRNA-1
Gene Sequence

>Msa1100570

ATGCTTAAACATCGCGACGATGATGAGTGTGAGGGGAAAGGATTTTACACATACGAAGCATTTGTTGAAGCTGCCAAAACATTCCCCAGGTTCGGCAACGAAGGAGACACTCAAGAGATTAAAAGAGAAATTGCTGCTTTTTTTGGTCAAAGCTCTCACAAAACAAGAGGTGGATCACCTCTTAGCCCCTATGACCGGGGATATTTCTTTGTGAATGAAACGAATCCCATCGAAGAAGACTACTGCGAACCAAGCTCTAAATTTCCATGTGTTCCTGGGAAGCAATACTACGAGAGGGGTCCGCTTCAGATCAAAGGGAATAAAATATATGGAGAATGTGGAAGTATAATAGGTGTTGATTTGCTCAATAACCCAGATCTCATTGCCACAGACCCTGTTATATCCTTCAAGAGTGCTATATGGTTTTGGATGAGAACTCCTCCACCTTTCCCTTTCCAAAAGGCTGGAAGGAATGCGCGACCGGGTGGCTGGATGTAA

Protein sequence

>Msa1100570

MLKHRDDDECEGKGFYTYEAFVEAAKTFPRFGNEGDTQEIKREIAAFFGQSSHKTRGGSPLSPYDRGYFFVNETNPIEEDYCEPSSKFPCVPGKQYYERGPLQIKGNKIYGECGSIIGVDLLNNPDLIATDPVISFKSAIWFWMRTPPPFPFQKAGRNARPGGWM*