AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1136050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136050 MtrunA17_Chr3g0145981 63.636 154 52 2 1 151 103 255 3.61e-66 205
Msa1136050 MtrunA17_Chr8g0370851 52.381 147 65 3 1 144 92 236 2.04e-51 166
Msa1136050 MtrunA17_Chr8g0370821 51.701 147 66 3 1 144 103 247 1.28e-49 162
Msa1136050 MtrunA17_Chr8g0370831 48.299 147 58 2 1 144 94 225 6.74e-43 144
Msa1136050 MtrunA17_Chr7g0270841 43.226 155 83 3 1 151 48 201 5.40e-40 135
Msa1136050 MtrunA17_Chr2g0329791 39.716 141 72 3 7 145 98 227 2.20e-29 108
Msa1136050 MtrunA17_Chr2g0329781 38.298 141 74 3 7 145 78 207 2.34e-29 108
Msa1136050 MtrunA17_Chr2g0294721 34.839 155 92 5 1 148 68 220 1.12e-23 94.4
Msa1136050 MtrunA17_Chr1g0206111 33.775 151 92 4 1 144 75 224 1.13e-21 89.0
Msa1136050 MtrunA17_Chr7g0221751 33.113 151 93 4 1 144 72 221 1.99e-21 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136050 AT3G12500.1 60.390 154 57 2 1 151 98 250 2.33e-62 195
Msa1136050 AT4G01700.1 47.368 152 75 3 4 151 53 203 1.02e-44 148
Msa1136050 AT1G02360.1 42.581 155 84 3 1 151 42 195 2.13e-39 134
Msa1136050 AT1G02360.3 43.421 152 81 3 4 151 97 247 4.97e-39 135
Msa1136050 AT1G02360.2 43.421 152 81 3 4 151 91 241 5.47e-39 134
Msa1136050 AT3G54420.1 41.060 151 76 3 8 156 90 229 1.50e-33 119
Msa1136050 AT2G43590.1 36.306 157 83 4 2 156 80 221 3.65e-33 118
Msa1136050 AT2G43580.1 33.333 156 89 2 2 156 81 222 6.06e-30 110
Msa1136050 AT2G43610.1 36.129 155 83 3 2 156 100 238 9.42e-28 104
Msa1136050 AT2G43620.1 36.129 155 83 3 2 156 102 240 5.48e-27 102
Msa1136050 AT1G56680.1 38.926 149 74 4 2 149 96 228 8.77e-26 99.4
Msa1136050 AT1G56680.2 39.860 143 69 4 2 143 119 245 1.47e-25 99.4
Msa1136050 AT2G43600.1 35.897 156 82 4 2 156 92 230 5.57e-24 94.7
Msa1136050 AT1G05850.1 35.762 151 89 4 1 144 72 221 8.55e-24 94.7
Msa1136050 AT1G05850.2 35.762 151 89 4 1 144 72 221 8.55e-24 94.7
Msa1136050 AT2G43600.2 35.897 156 82 4 2 156 113 251 9.70e-24 94.4
Msa1136050 AT2G43570.1 36.242 149 72 6 1 143 89 220 8.48e-23 91.7
Msa1136050 AT3G16920.1 32.903 155 95 5 1 148 80 232 1.67e-21 89.0
Msa1136050 AT3G16920.2 32.903 155 95 5 1 148 104 256 2.11e-21 89.0
Msa1136050 AT3G47540.1 27.152 151 81 6 8 156 48 171 3.33e-11 59.3
Msa1136050 AT3G47540.2 27.517 149 79 6 10 156 50 171 4.88e-11 59.7

Find 44 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGAGTGCTATATGGTTT+TGG 0.289569 7_4:-4005234 Msa1136050:CDS
TCTAAATTTCCATGTGTTCC+TGG 0.293914 7_4:-4005385 Msa1136050:CDS
GTCTCCTTCATTGCCGAACC+TGG 0.310175 7_4:+4005555 None:intergenic
ATCAAAGGGAATAAAATATA+TGG 0.311345 7_4:-4005331 Msa1136050:CDS
GGAGAATGTGGAAGTATAAT+AGG 0.323922 7_4:-4005310 Msa1136050:CDS
GCCGAACCTGGGGAATGTTT+TGG 0.347989 7_4:+4005567 None:intergenic
GGGTCTGTGGCAATGAGATC+TGG 0.352495 7_4:+4005268 None:intergenic
GCACTCTTGAAGGATATAAC+AGG 0.377927 7_4:+4005247 None:intergenic
TATCCTTCAAGAGTGCTATA+TGG 0.418078 7_4:-4005240 Msa1136050:CDS
AAACCATATAGCACTCTTGA+AGG 0.434167 7_4:+4005237 None:intergenic
GCCAAAACATTCCCCAGGTT+CGG 0.455985 7_4:-4005568 Msa1136050:CDS
CTAAATTTCCATGTGTTCCT+GGG 0.462737 7_4:-4005384 Msa1136050:CDS
ACACATGGAAATTTAGAGCT+TGG 0.463623 7_4:+4005391 None:intergenic
TTCGCAGTAGTCTTCTTCGA+TGG 0.468797 7_4:+4005414 None:intergenic
CTCTCGTAGTATTGCTTCCC+AGG 0.474080 7_4:+4005367 None:intergenic
CAAGATGGCTTTACTTGGAA+AGG 0.478902 7_4:+4005190 None:intergenic
TTCCCCAGGTTCGGCAATGA+AGG 0.485902 7_4:-4005559 Msa1136050:CDS
TCGCAGTAGTCTTCTTCGAT+GGG 0.491948 7_4:+4005415 None:intergenic
AGAGGAGGTTTCCAGGATAT+GGG 0.498919 7_4:-4005117 Msa1136050:intron
AAGATGGCTTTACTTGGAAA+GGG 0.503243 7_4:+4005191 None:intergenic
TTATTCCCTTTGATCTGAAG+CGG 0.516176 7_4:+4005340 None:intergenic
AATAAAATATATGGAGAATG+TGG 0.517331 7_4:-4005322 Msa1136050:CDS
AGGGGTCCGCTTCAGATCAA+AGG 0.525246 7_4:-4005346 Msa1136050:CDS
TCTCCTTCATTGCCGAACCT+GGG 0.529122 7_4:+4005556 None:intergenic
GGCTTTACTTGGAAAGGGAA+AGG 0.540330 7_4:+4005196 None:intergenic
GGTCTGTGGCAATGAGATCT+GGG 0.547304 7_4:+4005269 None:intergenic
ACTTGGAAAGGGAAAGGTGG+AGG 0.555116 7_4:+4005202 None:intergenic
GGGGTCCGCTTCAGATCAAA+GGG 0.557701 7_4:-4005345 Msa1136050:CDS
AAGCTGCCAAAACATTCCCC+AGG 0.566465 7_4:-4005573 Msa1136050:CDS
GATGATGAGTGTGAGGGGAA+AGG 0.572756 7_4:-4005622 Msa1136050:CDS
AGCTCTCACAAAACAAGAGG+TGG 0.574719 7_4:-4005490 Msa1136050:CDS
CTCCTTCATTGCCGAACCTG+GGG 0.576604 7_4:+4005557 None:intergenic
TATTGCTTCCCAGGAACACA+TGG 0.580157 7_4:+4005376 None:intergenic
CACTCTTGAAGGATATAACA+GGG 0.603547 7_4:+4005248 None:intergenic
CGCGACGATGATGAGTGTGA+GGG 0.608511 7_4:-4005628 Msa1136050:CDS
AGCTTCAACAAATGCTTCGT+AGG 0.613992 7_4:+4005591 None:intergenic
TCGCGACGATGATGAGTGTG+AGG 0.615130 7_4:-4005629 Msa1136050:CDS
GAAGGATATAACAGGGTCTG+TGG 0.619235 7_4:+4005255 None:intergenic
CTGGGAAGCAATACTACGAG+AGG 0.622203 7_4:-4005366 Msa1136050:CDS
CAAAGCTCTCACAAAACAAG+AGG 0.630156 7_4:-4005493 Msa1136050:CDS
TGGGAAGCAATACTACGAGA+GGG 0.631128 7_4:-4005365 Msa1136050:CDS
TTTACTTGGAAAGGGAAAGG+TGG 0.645661 7_4:+4005199 None:intergenic
GCGACGATGATGAGTGTGAG+GGG 0.672599 7_4:-4005627 Msa1136050:CDS
GGGAAGCAATACTACGAGAG+GGG 0.685899 7_4:-4005364 Msa1136050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAAGGGAATAAAATATA+TGG - chr7_4:4005422-4005441 Msa1136050:CDS 20.0%
!! AATAAAATATATGGAGAATG+TGG - chr7_4:4005431-4005450 Msa1136050:CDS 20.0%
! AAGAAATATCCCCAAAAATA+GGG + chr7_4:4005302-4005321 None:intergenic 25.0%
! AAAGAAATATCCCCAAAAAT+AGG + chr7_4:4005303-4005322 None:intergenic 25.0%
! GACTAAAATCAAAATCAGTA+GGG + chr7_4:4005616-4005635 None:intergenic 25.0%
! TGACTAAAATCAAAATCAGT+AGG + chr7_4:4005617-4005636 None:intergenic 25.0%
AGAAATATCCCCAAAAATAG+GGG + chr7_4:4005301-4005320 None:intergenic 30.0%
!!! CTGATTTTGATTTTAGTCAG+AGG - chr7_4:4005618-4005637 Msa1136050:CDS 30.0%
!!! ATTTTGATTTTAGTCAGAGG+AGG - chr7_4:4005621-4005640 Msa1136050:CDS 30.0%
! ACACATGGAAATTTAGAGCT+TGG + chr7_4:4005365-4005384 None:intergenic 35.0%
TCTAAATTTCCATGTGTTCC+TGG - chr7_4:4005368-4005387 Msa1136050:CDS 35.0%
CTAAATTTCCATGTGTTCCT+GGG - chr7_4:4005369-4005388 Msa1136050:CDS 35.0%
TTATTCCCTTTGATCTGAAG+CGG + chr7_4:4005416-4005435 None:intergenic 35.0%
! GGAGAATGTGGAAGTATAAT+AGG - chr7_4:4005443-4005462 Msa1136050:CDS 35.0%
CACTCTTGAAGGATATAACA+GGG + chr7_4:4005508-4005527 None:intergenic 35.0%
!! TATCCTTCAAGAGTGCTATA+TGG - chr7_4:4005513-4005532 Msa1136050:CDS 35.0%
AAACCATATAGCACTCTTGA+AGG + chr7_4:4005519-4005538 None:intergenic 35.0%
!!! TCAAGAGTGCTATATGGTTT+TGG - chr7_4:4005519-4005538 Msa1136050:CDS 35.0%
! AAGATGGCTTTACTTGGAAA+GGG + chr7_4:4005565-4005584 None:intergenic 35.0%
AGCTTCAACAAATGCTTCGT+AGG + chr7_4:4005165-4005184 None:intergenic 40.0%
!!! AGAGACAGTGCTGCTTTTTT+TGG - chr7_4:4005236-4005255 Msa1136050:CDS 40.0%
CAAAGCTCTCACAAAACAAG+AGG - chr7_4:4005260-4005279 Msa1136050:CDS 40.0%
!! ACCTCTTAGCCCCTATTTTT+GGG - chr7_4:4005289-4005308 Msa1136050:CDS 40.0%
GCACTCTTGAAGGATATAAC+AGG + chr7_4:4005509-4005528 None:intergenic 40.0%
TTTACTTGGAAAGGGAAAGG+TGG + chr7_4:4005557-4005576 None:intergenic 40.0%
! CAAGATGGCTTTACTTGGAA+AGG + chr7_4:4005566-4005585 None:intergenic 40.0%
AGCTCTCACAAAACAAGAGG+TGG - chr7_4:4005263-4005282 Msa1136050:CDS 45.0%
! CACCTCTTAGCCCCTATTTT+TGG - chr7_4:4005288-4005307 Msa1136050:CDS 45.0%
!!! CCTCTTAGCCCCTATTTTTG+GGG - chr7_4:4005290-4005309 Msa1136050:CDS 45.0%
TCGCAGTAGTCTTCTTCGAT+GGG + chr7_4:4005341-4005360 None:intergenic 45.0%
TTCGCAGTAGTCTTCTTCGA+TGG + chr7_4:4005342-4005361 None:intergenic 45.0%
TATTGCTTCCCAGGAACACA+TGG + chr7_4:4005380-4005399 None:intergenic 45.0%
TGGGAAGCAATACTACGAGA+GGG - chr7_4:4005388-4005407 Msa1136050:CDS 45.0%
GAAGGATATAACAGGGTCTG+TGG + chr7_4:4005501-4005520 None:intergenic 45.0%
GGCTTTACTTGGAAAGGGAA+AGG + chr7_4:4005560-4005579 None:intergenic 45.0%
! CATGGCAAGATGGCTTTACT+TGG + chr7_4:4005571-4005590 None:intergenic 45.0%
GTTATAGCGTCATGGCAAGA+TGG + chr7_4:4005581-4005600 None:intergenic 45.0%
TCTTGCCATGACGCTATAAC+CGG - chr7_4:4005581-4005600 Msa1136050:CDS 45.0%
TTTAGTCAGAGGAGGTTTCC+AGG - chr7_4:4005629-4005648 Msa1136050:CDS 45.0%
AGAGGAGGTTTCCAGGATAT+GGG - chr7_4:4005636-4005655 Msa1136050:CDS 45.0%
GATGATGAGTGTGAGGGGAA+AGG - chr7_4:4005131-4005150 Msa1136050:intron 50.0%
AAGCTGCCAAAACATTCCCC+AGG - chr7_4:4005180-4005199 Msa1136050:intron 50.0%
GCCAAAACATTCCCCAGGTT+CGG - chr7_4:4005185-4005204 Msa1136050:intron 50.0%
TCTCCTTCATTGCCGAACCT+GGG + chr7_4:4005200-4005219 None:intergenic 50.0%
CCCCAAAAATAGGGGCTAAG+AGG + chr7_4:4005293-4005312 None:intergenic 50.0%
CTCTCGTAGTATTGCTTCCC+AGG + chr7_4:4005389-4005408 None:intergenic 50.0%
CTGGGAAGCAATACTACGAG+AGG - chr7_4:4005387-4005406 Msa1136050:CDS 50.0%
GGGAAGCAATACTACGAGAG+GGG - chr7_4:4005389-4005408 Msa1136050:CDS 50.0%
GGTCTGTGGCAATGAGATCT+GGG + chr7_4:4005487-4005506 None:intergenic 50.0%
ACTTGGAAAGGGAAAGGTGG+AGG + chr7_4:4005554-4005573 None:intergenic 50.0%
ATCGTCCGGTTATAGCGTCA+TGG + chr7_4:4005589-4005608 None:intergenic 50.0%
ATGACGCTATAACCGGACGA+TGG - chr7_4:4005588-4005607 Msa1136050:CDS 50.0%
ATCAGTAGGGATCCATCGTC+CGG + chr7_4:4005603-4005622 None:intergenic 50.0%
CAGAGGAGGTTTCCAGGATA+TGG - chr7_4:4005635-4005654 Msa1136050:CDS 50.0%
TCGCGACGATGATGAGTGTG+AGG - chr7_4:4005124-4005143 Msa1136050:intron 55.0%
CGCGACGATGATGAGTGTGA+GGG - chr7_4:4005125-4005144 Msa1136050:intron 55.0%
GCGACGATGATGAGTGTGAG+GGG - chr7_4:4005126-4005145 Msa1136050:intron 55.0%
! GCCGAACCTGGGGAATGTTT+TGG + chr7_4:4005189-4005208 None:intergenic 55.0%
TTCCCCAGGTTCGGCAATGA+AGG - chr7_4:4005194-4005213 Msa1136050:intron 55.0%
CTCCTTCATTGCCGAACCTG+GGG + chr7_4:4005199-4005218 None:intergenic 55.0%
GTCTCCTTCATTGCCGAACC+TGG + chr7_4:4005201-4005220 None:intergenic 55.0%
AGGGGTCCGCTTCAGATCAA+AGG - chr7_4:4005407-4005426 Msa1136050:CDS 55.0%
GGGGTCCGCTTCAGATCAAA+GGG - chr7_4:4005408-4005427 Msa1136050:CDS 55.0%
GGGTCTGTGGCAATGAGATC+TGG + chr7_4:4005488-4005507 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 4005113 4005662 4005113 ID=Msa1136050;Name=Msa1136050
chr7_4 mRNA 4005113 4005662 4005113 ID=Msa1136050-mRNA-1;Parent=Msa1136050;Name=Msa1136050-mRNA-1;_AED=0.36;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|156
chr7_4 exon 4005204 4005662 4005204 ID=Msa1136050-mRNA-1:exon:1097;Parent=Msa1136050-mRNA-1
chr7_4 exon 4005113 4005124 4005113 ID=Msa1136050-mRNA-1:exon:1096;Parent=Msa1136050-mRNA-1
chr7_4 CDS 4005204 4005662 4005204 ID=Msa1136050-mRNA-1:cds;Parent=Msa1136050-mRNA-1
chr7_4 CDS 4005113 4005124 4005113 ID=Msa1136050-mRNA-1:cds;Parent=Msa1136050-mRNA-1
Gene Sequence

>Msa1136050

ATGCTTAAACATCGCGACGATGATGAGTGTGAGGGGAAAGGATTTTACACCTACGAAGCATTTGTTGAAGCTGCCAAAACATTCCCCAGGTTCGGCAATGAAGGAGACACTCAAGAGATTAAAAGAGACAGTGCTGCTTTTTTTGGTCAAAGCTCTCACAAAACAAGAGGTGGATCACCTCTTAGCCCCTATTTTTGGGGATATTTCTTTGTGAATGAAACGAATCCCATCGAAGAAGACTACTGCGAACCAAGCTCTAAATTTCCATGTGTTCCTGGGAAGCAATACTACGAGAGGGGTCCGCTTCAGATCAAAGGGAATAAAATATATGGAGAATGTGGAAGTATAATAGGTGTTGATTTGCTCAATAACCCAGATCTCATTGCCACAGACCCTGTTATATCCTTCAAGAGTGCTATATGGTTTTGGATGAGAACTCCTCCACCTTTCCCTTTCCAAGATATGGGATGA

Protein sequence

>Msa1136050

MLKHRDDDECEGKGFYTYEAFVEAAKTFPRFGNEGDTQEIKRDSAAFFGQSSHKTRGGSPLSPYFWGYFFVNETNPIEEDYCEPSSKFPCVPGKQYYERGPLQIKGNKIYGECGSIIGVDLLNNPDLIATDPVISFKSAIWFWMRTPPPFPFQDMG*