Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1118810 | XP_013449498.1 | 86.364 | 88 | 11 | 1 | 1 | 88 | 1 | 87 | 4.10e-47 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1118810 | A0A072U289 | 86.364 | 88 | 11 | 1 | 1 | 88 | 1 | 87 | 1.96e-47 | 155 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1118810 | Msa1158610 | 0.829684 | 4.484663e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1118810 | MtrunA17_Chr7g0253291 | 86.364 | 88 | 11 | 1 | 1 | 88 | 1 | 87 | 3.77e-51 | 155 |
| Msa1118810 | MtrunA17_Chr5g0448111 | 59.302 | 86 | 31 | 2 | 1 | 84 | 1 | 84 | 4.61e-31 | 104 |
| Msa1118810 | MtrunA17_Chr6g0461441 | 51.220 | 82 | 36 | 2 | 9 | 88 | 19 | 98 | 4.31e-21 | 79.7 |
| Msa1118810 | MtrunA17_Chr5g0432481 | 35.955 | 89 | 40 | 2 | 1 | 76 | 1 | 85 | 9.00e-13 | 58.2 |
| Msa1118810 | MtrunA17_Chr5g0448171 | 38.750 | 80 | 46 | 1 | 1 | 80 | 1 | 77 | 2.70e-12 | 56.6 |
| Msa1118810 | MtrunA17_Chr3g0107031 | 39.024 | 82 | 44 | 2 | 1 | 76 | 1 | 82 | 6.34e-11 | 53.5 |
| Msa1118810 | MtrunA17_Chr3g0078131 | 32.967 | 91 | 44 | 2 | 1 | 80 | 1 | 85 | 9.81e-11 | 53.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1118810 | AT5G18150.1 | 55.952 | 84 | 29 | 2 | 1 | 84 | 1 | 76 | 2.88e-27 | 95.1 |
| Msa1118810 | AT5G14602.1 | 45.238 | 84 | 42 | 1 | 1 | 84 | 1 | 80 | 1.21e-21 | 80.9 |
| Msa1118810 | AT5G58375.1 | 45.679 | 81 | 39 | 2 | 1 | 78 | 1 | 79 | 5.24e-17 | 69.3 |
Find 24 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGATTAAGAATGGATTT+TGG | 0.215303 | 7_3:+67584722 | Msa1118810:intron |
| TTATCCAACAAGGATCATTT+TGG | 0.248312 | 7_3:+67584587 | Msa1118810:CDS |
| TGGAAGAAATGTGTCCTCTT+AGG | 0.322863 | 7_3:+67584450 | Msa1118810:exon |
| TCAATTTGTTGCCTTGGAAT+TGG | 0.340448 | 7_3:+67584430 | Msa1118810:five_prime_UTR |
| AAAATGATCCTTGTTGGATA+AGG | 0.362560 | 7_3:-67584585 | None:intergenic |
| TGGCCAGTGGGAGATATCTT+TGG | 0.389035 | 7_3:+67584758 | Msa1118810:CDS |
| AAAACCAAAATGATCCTTGT+TGG | 0.417618 | 7_3:-67584591 | None:intergenic |
| TAGTTGGACATGATTATTTG+TGG | 0.484852 | 7_3:-67584794 | None:intergenic |
| TGGTTTCAGATGATTAAGAA+TGG | 0.487583 | 7_3:+67584715 | Msa1118810:intron |
| GTCAAACTCCTGTGTGGTGG+AGG | 0.508255 | 7_3:-67584534 | None:intergenic |
| ACTCTTAAGAGTTCTGTAGT+TGG | 0.518236 | 7_3:-67584810 | None:intergenic |
| CTGTGTGGTGGAGGAGCTCA+CGG | 0.518529 | 7_3:-67584525 | None:intergenic |
| ACACATTTCTTCCAATTCCA+AGG | 0.529274 | 7_3:-67584441 | None:intergenic |
| AAGATCAAGAGGAACCTAAG+AGG | 0.559496 | 7_3:-67584464 | None:intergenic |
| CGATAAATCCTTATCCAACA+AGG | 0.578287 | 7_3:+67584577 | Msa1118810:CDS |
| TTTCCAAAGATATCTCCCAC+TGG | 0.618699 | 7_3:-67584761 | None:intergenic |
| GTATTAGTTGACATGGCCAG+TGG | 0.636543 | 7_3:+67584745 | Msa1118810:CDS |
| CGTGAGCTCCTCCACCACAC+AGG | 0.640612 | 7_3:+67584526 | Msa1118810:CDS |
| TATTAGTTGACATGGCCAGT+GGG | 0.647697 | 7_3:+67584746 | Msa1118810:CDS |
| GGTGGTCCACGCAAAATAAG+CGG | 0.650418 | 7_3:-67584504 | None:intergenic |
| GAAGTCAAACTCCTGTGTGG+TGG | 0.665207 | 7_3:-67584537 | None:intergenic |
| TGGATAAGGATTTATCGTTG+CGG | 0.668515 | 7_3:-67584571 | None:intergenic |
| TGCGAAGTCAAACTCCTGTG+TGG | 0.690647 | 7_3:-67584540 | None:intergenic |
| TGTGGTGGAGGAGCTCACGG+TGG | 0.767016 | 7_3:-67584522 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTACATAAAGAAAATG+TGG | - | chr7_3:67584635-67584654 | None:intergenic | 20.0% |
| !! | TTGACTTGAAATGTTAACTT+TGG | + | chr7_3:67584695-67584714 | Msa1118810:intron | 25.0% |
| !!! | AGATGATTAAGAATGGATTT+TGG | + | chr7_3:67584722-67584741 | Msa1118810:intron | 25.0% |
| !!! | GATGATTAAGAATGGATTTT+GGG | + | chr7_3:67584723-67584742 | Msa1118810:intron | 25.0% |
| !! | GATATCTTTGGAAAAATTTG+AGG | + | chr7_3:67584770-67584789 | Msa1118810:CDS | 25.0% |
| !! | GAAATTTTTGTCACATACAT+TGG | - | chr7_3:67584880-67584899 | None:intergenic | 25.0% |
| !!! | TTTTCATCAATTTGTTGCCT+TGG | + | chr7_3:67584424-67584443 | Msa1118810:five_prime_UTR | 30.0% |
| AAAATGATCCTTGTTGGATA+AGG | - | chr7_3:67584588-67584607 | None:intergenic | 30.0% | |
| ! | TTATCCAACAAGGATCATTT+TGG | + | chr7_3:67584587-67584606 | Msa1118810:CDS | 30.0% |
| AAAACCAAAATGATCCTTGT+TGG | - | chr7_3:67584594-67584613 | None:intergenic | 30.0% | |
| !!! | GGATCATTTTGGTTTTATCA+AGG | + | chr7_3:67584598-67584617 | Msa1118810:CDS | 30.0% |
| ACATAAAGAAAATGTGGAAC+CGG | - | chr7_3:67584629-67584648 | None:intergenic | 30.0% | |
| AATACAAGCACGAAAAAGAT+AGG | - | chr7_3:67584677-67584696 | None:intergenic | 30.0% | |
| TGGTTTCAGATGATTAAGAA+TGG | + | chr7_3:67584715-67584734 | Msa1118810:intron | 30.0% | |
| !! | ATTTTGGGTATTAGTTGACA+TGG | + | chr7_3:67584738-67584757 | Msa1118810:CDS | 30.0% |
| TAGTTGGACATGATTATTTG+TGG | - | chr7_3:67584797-67584816 | None:intergenic | 30.0% | |
| !! | TCAATTTGTTGCCTTGGAAT+TGG | + | chr7_3:67584430-67584449 | Msa1118810:five_prime_UTR | 35.0% |
| ACACATTTCTTCCAATTCCA+AGG | - | chr7_3:67584444-67584463 | None:intergenic | 35.0% | |
| TGGATAAGGATTTATCGTTG+CGG | - | chr7_3:67584574-67584593 | None:intergenic | 35.0% | |
| CGATAAATCCTTATCCAACA+AGG | + | chr7_3:67584577-67584596 | Msa1118810:CDS | 35.0% | |
| !!! | TGGTTTTATCAAGGTATTCC+CGG | + | chr7_3:67584607-67584626 | Msa1118810:intron | 35.0% |
| CATAAAGAAAATGTGGAACC+GGG | - | chr7_3:67584628-67584647 | None:intergenic | 35.0% | |
| ACTCTTAAGAGTTCTGTAGT+TGG | - | chr7_3:67584813-67584832 | None:intergenic | 35.0% | |
| TGGAAGAAATGTGTCCTCTT+AGG | + | chr7_3:67584450-67584469 | Msa1118810:exon | 40.0% | |
| AAGATCAAGAGGAACCTAAG+AGG | - | chr7_3:67584467-67584486 | None:intergenic | 40.0% | |
| CAGCTGAGAAAAAGATCAAG+AGG | - | chr7_3:67584478-67584497 | None:intergenic | 40.0% | |
| ! | GATCTTTTTCTCAGCTGTGT+TGG | + | chr7_3:67584481-67584500 | Msa1118810:CDS | 40.0% |
| TATTAGTTGACATGGCCAGT+GGG | + | chr7_3:67584746-67584765 | Msa1118810:CDS | 40.0% | |
| TTTCCAAAGATATCTCCCAC+TGG | - | chr7_3:67584764-67584783 | None:intergenic | 40.0% | |
| GTATTAGTTGACATGGCCAG+TGG | + | chr7_3:67584745-67584764 | Msa1118810:CDS | 45.0% | |
| !!! | TGTTGGCCGCTTATTTTGCG+TGG | + | chr7_3:67584498-67584517 | Msa1118810:CDS | 50.0% |
| GGTGGTCCACGCAAAATAAG+CGG | - | chr7_3:67584507-67584526 | None:intergenic | 50.0% | |
| GAAGTCAAACTCCTGTGTGG+TGG | - | chr7_3:67584540-67584559 | None:intergenic | 50.0% | |
| TGCGAAGTCAAACTCCTGTG+TGG | - | chr7_3:67584543-67584562 | None:intergenic | 50.0% | |
| TGGCCAGTGGGAGATATCTT+TGG | + | chr7_3:67584758-67584777 | Msa1118810:CDS | 50.0% | |
| GTCAAACTCCTGTGTGGTGG+AGG | - | chr7_3:67584537-67584556 | None:intergenic | 55.0% | |
| ! | CTGTGTGGTGGAGGAGCTCA+CGG | - | chr7_3:67584528-67584547 | None:intergenic | 60.0% |
| ! | TGTGGTGGAGGAGCTCACGG+TGG | - | chr7_3:67584525-67584544 | None:intergenic | 65.0% |
| CGTGAGCTCCTCCACCACAC+AGG | + | chr7_3:67584526-67584545 | Msa1118810:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 67584376 | 67584921 | 67584376 | ID=Msa1118810;Name=Msa1118810 |
| chr7_3 | mRNA | 67584376 | 67584921 | 67584376 | ID=Msa1118810-mRNA-1;Parent=Msa1118810;Name=Msa1118810-mRNA-1;_AED=0.04;_eAED=0.04;_QI=82|1|1|1|1|1|2|93|88 |
| chr7_3 | exon | 67584376 | 67584619 | 67584376 | ID=Msa1118810-mRNA-1:exon:16627;Parent=Msa1118810-mRNA-1 |
| chr7_3 | exon | 67584724 | 67584921 | 67584724 | ID=Msa1118810-mRNA-1:exon:16628;Parent=Msa1118810-mRNA-1 |
| chr7_3 | five_prime_UTR | 67584376 | 67584457 | 67584376 | ID=Msa1118810-mRNA-1:five_prime_utr;Parent=Msa1118810-mRNA-1 |
| chr7_3 | CDS | 67584458 | 67584619 | 67584458 | ID=Msa1118810-mRNA-1:cds;Parent=Msa1118810-mRNA-1 |
| chr7_3 | CDS | 67584724 | 67584828 | 67584724 | ID=Msa1118810-mRNA-1:cds;Parent=Msa1118810-mRNA-1 |
| chr7_3 | three_prime_UTR | 67584829 | 67584921 | 67584829 | ID=Msa1118810-mRNA-1:three_prime_utr;Parent=Msa1118810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
