Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1158610 | XP_013449498.1 | 87.500 | 88 | 10 | 1 | 1 | 88 | 1 | 87 | 4.77e-48 | 157 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1158610 | A0A072U289 | 87.500 | 88 | 10 | 1 | 1 | 88 | 1 | 87 | 2.28e-48 | 157 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0481770 | Msa1158610 | 0.810126 | 1.345925e-50 | -8.615850e-47 |
| Msa1118810 | Msa1158610 | 0.829684 | 4.484663e-55 | -8.615850e-47 |
| Msa1158610 | Msa1346010 | 0.818418 | 1.980318e-52 | -8.615850e-47 |
| Msa1158610 | Msa1383790 | 0.825315 | 5.013568e-54 | -8.615850e-47 |
| Msa1158610 | Msa1453600 | 0.800203 | 1.609221e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1158610 | MtrunA17_Chr7g0253291 | 87.500 | 88 | 10 | 1 | 1 | 88 | 1 | 87 | 4.39e-52 | 157 |
| Msa1158610 | MtrunA17_Chr5g0448111 | 59.302 | 86 | 31 | 2 | 1 | 84 | 1 | 84 | 6.12e-31 | 104 |
| Msa1158610 | MtrunA17_Chr6g0461441 | 51.220 | 82 | 36 | 2 | 9 | 88 | 19 | 98 | 2.75e-21 | 80.1 |
| Msa1158610 | MtrunA17_Chr5g0448171 | 40.000 | 80 | 45 | 2 | 1 | 80 | 1 | 77 | 2.83e-13 | 59.3 |
| Msa1158610 | MtrunA17_Chr5g0432481 | 35.955 | 89 | 40 | 2 | 1 | 76 | 1 | 85 | 3.21e-12 | 57.0 |
| Msa1158610 | MtrunA17_Chr3g0107031 | 40.244 | 82 | 43 | 2 | 1 | 76 | 1 | 82 | 4.68e-11 | 53.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1158610 | AT5G18150.1 | 57.143 | 84 | 28 | 3 | 1 | 84 | 1 | 76 | 9.73e-27 | 93.6 |
| Msa1158610 | AT5G14602.1 | 45.238 | 84 | 42 | 1 | 1 | 84 | 1 | 80 | 3.74e-22 | 82.0 |
| Msa1158610 | AT5G58375.1 | 44.304 | 79 | 43 | 1 | 1 | 78 | 1 | 79 | 4.35e-17 | 69.3 |
Find 25 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGATTAAGAATGGATTT+TGG | 0.215303 | 7_4:-48019446 | Msa1158610:CDS |
| TCATCCAACAAGGATCATTT+TGG | 0.237826 | 7_4:-48019580 | Msa1158610:CDS |
| TGGCCAGTGGGAGGTATCTT+TGG | 0.321714 | 7_4:-48019410 | Msa1158610:CDS |
| TGGAAGAAATGTGTCCTCTT+AGG | 0.322863 | 7_4:-48019717 | Msa1158610:exon |
| TCAATTTGTTGCCTTGGAAT+TGG | 0.340448 | 7_4:-48019737 | Msa1158610:five_prime_UTR |
| AAAACCAAAATGATCCTTGT+TGG | 0.442824 | 7_4:+48019576 | None:intergenic |
| TAGTTGGACATGATTATTTG+TGG | 0.484852 | 7_4:+48019374 | None:intergenic |
| TGGTTTCAGATGATTAAGAA+TGG | 0.487583 | 7_4:-48019453 | Msa1158610:intron |
| AAAATGATCCTTGTTGGATG+AGG | 0.493557 | 7_4:+48019582 | None:intergenic |
| GTCAAACTCCTGTGTGGTGG+AGG | 0.508255 | 7_4:+48019633 | None:intergenic |
| ACTCTTAAGAGTTCTGTAGT+TGG | 0.518236 | 7_4:+48019358 | None:intergenic |
| CTGTGTGGTGGAGGAGCTCA+CGG | 0.518529 | 7_4:+48019642 | None:intergenic |
| ACACATTTCTTCCAATTCCA+AGG | 0.529274 | 7_4:+48019726 | None:intergenic |
| AAGATCAAGAGGAACCTAAG+AGG | 0.559496 | 7_4:+48019703 | None:intergenic |
| CGATAAATCCTCATCCAACA+AGG | 0.581880 | 7_4:-48019590 | Msa1158610:CDS |
| TTTCCAAAGATACCTCCCAC+TGG | 0.620026 | 7_4:+48019407 | None:intergenic |
| GTATTAGTTGACATGGCCAG+TGG | 0.636543 | 7_4:-48019423 | Msa1158610:CDS |
| TATTAGTTGACATGGCCAGT+GGG | 0.640261 | 7_4:-48019422 | Msa1158610:CDS |
| CGTGAGCTCCTCCACCACAC+AGG | 0.640612 | 7_4:-48019641 | Msa1158610:CDS |
| GGTGGTCCACGCAAAATAAG+CGG | 0.650418 | 7_4:+48019663 | None:intergenic |
| GAAGTCAAACTCCTGTGTGG+TGG | 0.665207 | 7_4:+48019630 | None:intergenic |
| TAGTTGACATGGCCAGTGGG+AGG | 0.679321 | 7_4:-48019419 | Msa1158610:CDS |
| TGCGAAGTCAAACTCCTGTG+TGG | 0.690647 | 7_4:+48019627 | None:intergenic |
| TGGATGAGGATTTATCGTTG+CGG | 0.701777 | 7_4:+48019596 | None:intergenic |
| TGTGGTGGAGGAGCTCACGG+TGG | 0.767016 | 7_4:+48019645 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAATTGCATAAAGAAAATG+TGG | + | chr7_4:48019528-48019547 | None:intergenic | 25.0% |
| !!! | ATTGACTTGAAATGTTACTT+TGG | - | chr7_4:48019587-48019606 | Msa1158610:CDS | 25.0% |
| !!! | AGATGATTAAGAATGGATTT+TGG | - | chr7_4:48019614-48019633 | Msa1158610:CDS | 25.0% |
| !!! | GATGATTAAGAATGGATTTT+GGG | - | chr7_4:48019615-48019634 | Msa1158610:CDS | 25.0% |
| !! | GAAATTTTTGTCACATACAT+TGG | + | chr7_4:48019772-48019791 | None:intergenic | 25.0% |
| !!! | TTTTCATCAATTTGTTGCCT+TGG | - | chr7_4:48019317-48019336 | Msa1158610:three_prime_UTR | 30.0% |
| AAAACCAAAATGATCCTTGT+TGG | + | chr7_4:48019487-48019506 | None:intergenic | 30.0% | |
| !!! | GGATCATTTTGGTTTTATCA+AGG | - | chr7_4:48019491-48019510 | Msa1158610:intron | 30.0% |
| AATACAAGCACGAAAAAGAT+AGG | + | chr7_4:48019570-48019589 | None:intergenic | 30.0% | |
| TGGTTTCAGATGATTAAGAA+TGG | - | chr7_4:48019607-48019626 | Msa1158610:CDS | 30.0% | |
| !! | ATTTTGGGTATTAGTTGACA+TGG | - | chr7_4:48019630-48019649 | Msa1158610:CDS | 30.0% |
| ! | GGTATCTTTGGAAAAATTTG+AGG | - | chr7_4:48019662-48019681 | Msa1158610:CDS | 30.0% |
| TAGTTGGACATGATTATTTG+TGG | + | chr7_4:48019689-48019708 | None:intergenic | 30.0% | |
| !! | TCAATTTGTTGCCTTGGAAT+TGG | - | chr7_4:48019323-48019342 | Msa1158610:three_prime_UTR | 35.0% |
| ACACATTTCTTCCAATTCCA+AGG | + | chr7_4:48019337-48019356 | None:intergenic | 35.0% | |
| AAAATGATCCTTGTTGGATG+AGG | + | chr7_4:48019481-48019500 | None:intergenic | 35.0% | |
| ! | TCATCCAACAAGGATCATTT+TGG | - | chr7_4:48019480-48019499 | Msa1158610:intron | 35.0% |
| !!! | TGGTTTTATCAAGGTATTCC+CGG | - | chr7_4:48019500-48019519 | Msa1158610:intron | 35.0% |
| CATAAAGAAAATGTGGAACC+GGG | + | chr7_4:48019521-48019540 | None:intergenic | 35.0% | |
| GCATAAAGAAAATGTGGAAC+CGG | + | chr7_4:48019522-48019541 | None:intergenic | 35.0% | |
| ACTCTTAAGAGTTCTGTAGT+TGG | + | chr7_4:48019705-48019724 | None:intergenic | 35.0% | |
| TGGAAGAAATGTGTCCTCTT+AGG | - | chr7_4:48019343-48019362 | Msa1158610:exon | 40.0% | |
| AAGATCAAGAGGAACCTAAG+AGG | + | chr7_4:48019360-48019379 | None:intergenic | 40.0% | |
| CAGCTGAGAAAAAGATCAAG+AGG | + | chr7_4:48019371-48019390 | None:intergenic | 40.0% | |
| ! | GATCTTTTTCTCAGCTGTGT+TGG | - | chr7_4:48019374-48019393 | Msa1158610:CDS | 40.0% |
| TGGATGAGGATTTATCGTTG+CGG | + | chr7_4:48019467-48019486 | None:intergenic | 40.0% | |
| CGATAAATCCTCATCCAACA+AGG | - | chr7_4:48019470-48019489 | Msa1158610:intron | 40.0% | |
| TATTAGTTGACATGGCCAGT+GGG | - | chr7_4:48019638-48019657 | Msa1158610:CDS | 40.0% | |
| GTATTAGTTGACATGGCCAG+TGG | - | chr7_4:48019637-48019656 | Msa1158610:CDS | 45.0% | |
| TTTCCAAAGATACCTCCCAC+TGG | + | chr7_4:48019656-48019675 | None:intergenic | 45.0% | |
| !!! | TGTTGGCCGCTTATTTTGCG+TGG | - | chr7_4:48019391-48019410 | Msa1158610:CDS | 50.0% |
| GGTGGTCCACGCAAAATAAG+CGG | + | chr7_4:48019400-48019419 | None:intergenic | 50.0% | |
| GAAGTCAAACTCCTGTGTGG+TGG | + | chr7_4:48019433-48019452 | None:intergenic | 50.0% | |
| TGCGAAGTCAAACTCCTGTG+TGG | + | chr7_4:48019436-48019455 | None:intergenic | 50.0% | |
| GTCAAACTCCTGTGTGGTGG+AGG | + | chr7_4:48019430-48019449 | None:intergenic | 55.0% | |
| TAGTTGACATGGCCAGTGGG+AGG | - | chr7_4:48019641-48019660 | Msa1158610:CDS | 55.0% | |
| !! | TGGCCAGTGGGAGGTATCTT+TGG | - | chr7_4:48019650-48019669 | Msa1158610:CDS | 55.0% |
| ! | CTGTGTGGTGGAGGAGCTCA+CGG | + | chr7_4:48019421-48019440 | None:intergenic | 60.0% |
| ! | TGTGGTGGAGGAGCTCACGG+TGG | + | chr7_4:48019418-48019437 | None:intergenic | 65.0% |
| CGTGAGCTCCTCCACCACAC+AGG | - | chr7_4:48019419-48019438 | Msa1158610:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 48019269 | 48019813 | 48019269 | ID=Msa1158610;Name=Msa1158610 |
| chr7_4 | mRNA | 48019269 | 48019813 | 48019269 | ID=Msa1158610-mRNA-1;Parent=Msa1158610;Name=Msa1158610-mRNA-1;_AED=0.01;_eAED=0.01;_QI=82|1|1|1|1|1|2|93|88 |
| chr7_4 | exon | 48019269 | 48019466 | 48019269 | ID=Msa1158610-mRNA-1:exon:12918;Parent=Msa1158610-mRNA-1 |
| chr7_4 | exon | 48019570 | 48019813 | 48019570 | ID=Msa1158610-mRNA-1:exon:12917;Parent=Msa1158610-mRNA-1 |
| chr7_4 | five_prime_UTR | 48019732 | 48019813 | 48019732 | ID=Msa1158610-mRNA-1:five_prime_utr;Parent=Msa1158610-mRNA-1 |
| chr7_4 | CDS | 48019570 | 48019731 | 48019570 | ID=Msa1158610-mRNA-1:cds;Parent=Msa1158610-mRNA-1 |
| chr7_4 | CDS | 48019362 | 48019466 | 48019362 | ID=Msa1158610-mRNA-1:cds;Parent=Msa1158610-mRNA-1 |
| chr7_4 | three_prime_UTR | 48019269 | 48019361 | 48019269 | ID=Msa1158610-mRNA-1:three_prime_utr;Parent=Msa1158610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
