AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1121350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1121350 MtrunA17_Chr7g0249931 88.095 84 10 0 1 84 311 394 2.60e-47 157
Msa1121350 MtrunA17_Chr7g0249981 70.238 84 25 0 1 84 295 378 2.53e-38 133
Msa1121350 MtrunA17_Chr7g0249961 66.667 87 29 0 1 87 88 174 6.42e-38 127
Msa1121350 MtrunA17_Chr7g0250001 71.429 84 24 0 1 84 276 359 2.14e-35 125
Msa1121350 MtrunA17_Chr6g0464881 60.241 83 33 0 2 84 284 366 1.59e-32 117
Msa1121350 MtrunA17_Chr6g0464871 63.855 83 30 0 2 84 296 378 7.40e-32 115
Msa1121350 MtrunA17_Chr6g0464921 61.039 77 29 1 1 76 283 359 5.34e-31 113
Msa1121350 MtrunA17_Chr6g0464841 59.524 84 34 0 1 84 283 366 1.35e-29 109
Msa1121350 MtrunA17_Chr6g0464861 59.524 84 34 0 1 84 282 365 1.67e-29 108
Msa1121350 MtrunA17_Chr5g0414331 55.682 88 38 1 1 87 281 368 1.39e-27 103
Msa1121350 MtrunA17_Chr5g0414321 54.545 88 39 1 1 87 279 366 3.12e-26 100
Msa1121350 MtrunA17_Chr1g0207981 52.564 78 32 2 4 76 299 376 5.77e-23 91.3
Msa1121350 MtrunA17_Chr8g0377941 51.948 77 33 2 4 79 292 365 6.29e-20 82.8
Msa1121350 MtrunA17_Chr8g0377921 49.367 79 37 1 4 79 289 367 1.98e-19 81.3
Msa1121350 MtrunA17_Chr3g0098111 45.455 88 41 2 4 84 292 379 2.23e-19 81.3
Msa1121350 MtrunA17_Chr8g0380531 46.835 79 39 1 1 76 283 361 4.85e-19 80.1
Msa1121350 MtrunA17_Chr3g0122001 40.625 96 44 2 1 84 289 383 5.87e-19 80.1
Msa1121350 MtrunA17_Chr3g0122001 40.625 96 44 2 1 84 769 863 8.50e-19 79.7
Msa1121350 MtrunA17_Chr5g0429681 43.956 91 42 2 3 84 322 412 7.32e-19 79.7
Msa1121350 MtrunA17_Chr7g0250021 83.333 42 7 0 1 42 283 324 9.58e-19 78.6
Msa1121350 MtrunA17_Chr4g0044481 48.148 81 37 3 1 76 284 364 1.17e-18 79.0
Msa1121350 MtrunA17_Chr3g0121991 39.796 98 42 2 1 84 362 456 3.01e-18 77.8
Msa1121350 MtrunA17_Chr1g0207931 43.182 88 43 2 4 84 320 407 1.85e-17 75.9
Msa1121350 MtrunA17_Chr5g0448551 36.782 87 52 1 4 87 283 369 2.89e-17 75.1
Msa1121350 MtrunA17_Chr1g0161351 44.318 88 42 2 4 84 320 407 3.67e-17 74.7
Msa1121350 MtrunA17_Chr4g0053321 42.268 97 46 3 1 87 298 394 4.24e-17 74.7
Msa1121350 MtrunA17_Chr7g0243631 41.304 92 46 3 1 84 296 387 4.49e-17 74.7
Msa1121350 MtrunA17_Chr5g0429711 43.529 85 44 2 3 84 289 372 4.61e-17 74.7
Msa1121350 MtrunA17_Chr5g0442491 34.694 98 47 2 4 84 283 380 4.80e-17 74.3
Msa1121350 MtrunA17_Chr5g0401721 41.463 82 41 2 4 78 295 376 4.94e-17 74.3
Msa1121350 MtrunA17_Chr2g0302961 36.735 98 48 2 1 84 279 376 5.05e-17 74.3
Msa1121350 MtrunA17_Chr5g0393591 48.101 79 34 2 4 75 277 355 8.82e-17 73.6
Msa1121350 MtrunA17_Chr5g0442601 34.000 100 49 2 2 84 682 781 1.01e-16 73.6
Msa1121350 MtrunA17_Chr5g0442601 33.673 98 48 3 4 84 211 308 3.25e-13 63.5
Msa1121350 MtrunA17_Chr5g0412111 42.697 89 47 2 3 87 293 381 1.17e-16 73.6
Msa1121350 MtrunA17_Chr1g0207861 41.758 91 46 2 1 84 288 378 1.29e-16 73.2
Msa1121350 MtrunA17_Chr7g0243681 39.130 92 48 2 1 84 289 380 1.36e-16 73.2
Msa1121350 MtrunA17_Chr4g0053501 45.349 86 37 3 1 76 283 368 1.40e-16 73.2
Msa1121350 MtrunA17_Chr8g0366391 45.679 81 39 3 1 76 296 376 1.87e-16 72.8
Msa1121350 MtrunA17_Chr8g0382021 38.776 98 44 3 3 84 301 398 2.37e-16 72.4
Msa1121350 MtrunA17_Chr5g0448521 38.000 100 45 3 2 84 274 373 2.59e-16 72.4
Msa1121350 MtrunA17_Chr1g0207951 42.353 85 46 2 1 84 936 1018 3.15e-16 72.4
Msa1121350 MtrunA17_Chr1g0207951 41.250 80 43 2 6 84 406 482 1.94e-15 70.1
Msa1121350 MtrunA17_Chr8g0336651 39.000 100 44 3 2 84 277 376 3.19e-16 72.4
Msa1121350 MtrunA17_Chr8g0336651 38.095 84 37 2 2 84 617 686 2.32e-12 61.2
Msa1121350 MtrunA17_Chr8g0336651 35.577 104 46 4 2 84 1052 1155 1.98e-11 58.5
Msa1121350 MtrunA17_Chr1g0207831 42.500 80 43 2 6 84 295 372 3.44e-16 72.0
Msa1121350 MtrunA17_Chr7g0243801 39.130 92 48 2 1 84 287 378 3.47e-16 72.0
Msa1121350 MtrunA17_Chr8g0336741 38.000 100 45 3 2 84 284 383 3.79e-16 72.0
Msa1121350 MtrunA17_Chr7g0243771 39.130 92 48 2 1 84 286 377 3.86e-16 72.0
Msa1121350 MtrunA17_Chr5g0442561 33.663 101 50 2 1 84 278 378 5.29e-16 71.6
Msa1121350 MtrunA17_Chr2g0302991 35.714 98 49 2 1 84 276 373 6.70e-16 71.2
Msa1121350 MtrunA17_Chr7g0235051 38.043 92 49 2 1 84 264 355 6.78e-16 71.2
Msa1121350 MtrunA17_Chr3g0106251 48.611 72 33 2 3 74 124 191 8.09e-16 70.5
Msa1121350 MtrunA17_Chr6g0454371 38.202 89 50 2 4 87 1 89 1.55e-15 67.8
Msa1121350 MtrunA17_Chr5g0412131 43.529 85 44 2 7 87 305 389 1.67e-15 70.1
Msa1121350 MtrunA17_Chr5g0442521 31.683 101 51 2 2 84 281 381 1.67e-15 70.1
Msa1121350 MtrunA17_Chr5g0412101 42.045 88 47 2 4 87 303 390 1.95e-15 70.1
Msa1121350 MtrunA17_Chr6g0459311 39.362 94 40 3 2 78 283 376 2.01e-15 69.7
Msa1121350 MtrunA17_Chr4g0044511 39.535 86 42 3 1 76 317 402 2.05e-15 69.7
Msa1121350 MtrunA17_Chr4g0066201 39.241 79 38 2 6 74 288 366 3.77e-15 68.9
Msa1121350 MtrunA17_Chr5g0442581 33.673 98 48 2 4 84 285 382 3.82e-15 68.9
Msa1121350 MtrunA17_Chr8g0365811 40.217 92 44 2 4 84 776 867 3.89e-15 69.3
Msa1121350 MtrunA17_Chr8g0365811 36.842 95 49 2 1 84 291 385 6.84e-15 68.6
Msa1121350 MtrunA17_Chr7g0243611 38.043 92 49 2 1 84 155 246 4.75e-15 68.2
Msa1121350 MtrunA17_Chr5g0401851 41.176 85 42 3 8 84 302 386 6.26e-15 68.6
Msa1121350 MtrunA17_Chr1g0207971 41.463 82 45 2 4 84 286 365 6.96e-15 68.2
Msa1121350 MtrunA17_Chr5g0442541 33.000 100 50 2 2 84 272 371 6.99e-15 68.2
Msa1121350 MtrunA17_Chr5g0431441 47.945 73 34 3 3 75 296 364 7.13e-15 68.2
Msa1121350 MtrunA17_Chr7g0243761 35.870 92 51 2 1 84 294 385 7.33e-15 68.2
Msa1121350 MtrunA17_Chr4g0066171 39.241 79 38 1 6 74 290 368 8.06e-15 68.2
Msa1121350 MtrunA17_Chr3g0079731 42.105 95 35 4 2 76 289 383 9.06e-15 68.2
Msa1121350 MtrunA17_Chr1g0159551 36.842 95 47 3 3 84 275 369 9.15e-15 68.2
Msa1121350 MtrunA17_Chr8g0355221 39.080 87 44 2 1 78 297 383 1.00e-14 67.8
Msa1121350 MtrunA17_Chr4g0072761 40.000 70 41 1 8 76 290 359 1.14e-14 67.8
Msa1121350 MtrunA17_Chr1g0173121 36.842 95 47 3 3 84 278 372 1.17e-14 67.8
Msa1121350 MtrunA17_Chr8g0335141 39.362 94 40 3 2 78 280 373 1.18e-14 67.8
Msa1121350 MtrunA17_Chr3g0118721 33.333 93 56 2 1 87 116 208 1.19e-14 67.0
Msa1121350 MtrunA17_Chr7g0217881 36.957 92 53 2 1 87 289 380 1.23e-14 67.8
Msa1121350 MtrunA17_Chr7g0232401 37.895 95 41 3 2 78 279 373 1.26e-14 67.8
Msa1121350 MtrunA17_Chr5g0431431 43.902 82 42 2 3 84 227 304 1.28e-14 67.4
Msa1121350 MtrunA17_Chr7g0243781 35.870 92 51 2 1 84 284 375 1.39e-14 67.4
Msa1121350 MtrunA17_Chr8g0336261 39.326 89 49 2 4 87 1 89 1.40e-14 64.3
Msa1121350 MtrunA17_Chr4g0044501 43.902 82 41 3 8 84 301 382 1.53e-14 67.4
Msa1121350 MtrunA17_Chr3g0106231 47.222 72 34 2 3 74 282 349 1.79e-14 67.0
Msa1121350 MtrunA17_Chr3g0118701 33.333 93 56 2 1 87 309 401 1.94e-14 67.0
Msa1121350 MtrunA17_Chr7g0218841 37.363 91 51 2 1 87 270 358 1.98e-14 67.0
Msa1121350 MtrunA17_Chr5g0426401 38.043 92 52 2 1 87 138 229 2.00e-14 66.6
Msa1121350 MtrunA17_Chr8g0336731 36.000 100 47 3 2 84 303 402 2.10e-14 67.0
Msa1121350 MtrunA17_Chr7g0232411 33.000 100 50 3 2 84 278 377 2.23e-14 67.0
Msa1121350 MtrunA17_Chr4g0060511 36.957 92 50 2 1 84 298 389 2.25e-14 67.0
Msa1121350 MtrunA17_Chr6g0486251 39.362 94 40 3 2 78 276 369 2.29e-14 67.0
Msa1121350 MtrunA17_Chr6g0486201 39.362 94 40 3 2 78 276 369 2.29e-14 67.0
Msa1121350 MtrunA17_Chr2g0278711 40.230 87 45 2 8 87 308 394 2.30e-14 67.0
Msa1121350 MtrunA17_Chr8g0361371 35.294 102 47 3 2 84 276 377 2.54e-14 66.6
Msa1121350 MtrunA17_Chr7g0218831 37.363 91 51 2 1 87 269 357 2.55e-14 66.6
Msa1121350 MtrunA17_Chr4g0072771 42.857 70 39 1 8 76 303 372 2.66e-14 66.6
Msa1121350 MtrunA17_Chr3g0118691 33.333 93 56 2 1 87 309 401 2.67e-14 66.6
Msa1121350 MtrunA17_Chr8g0373301 37.374 99 46 2 2 84 280 378 2.74e-14 66.6
Msa1121350 MtrunA17_Chr6g0454301 36.957 92 53 2 1 87 558 649 3.11e-14 66.6
Msa1121350 MtrunA17_Chr7g0243951 39.024 82 43 2 4 78 298 379 3.19e-14 66.6
Msa1121350 MtrunA17_Chr1g0165701 37.647 85 46 2 1 78 296 380 3.26e-14 66.6
Msa1121350 MtrunA17_Chr6g0453961 38.202 89 50 2 4 87 320 408 4.27e-14 66.2
Msa1121350 MtrunA17_Chr7g0218811 38.961 77 45 1 1 75 279 355 5.07e-14 65.9
Msa1121350 MtrunA17_Chr7g0232341 33.000 100 50 3 2 84 278 377 5.21e-14 65.9
Msa1121350 MtrunA17_Chr8g0335161 39.130 92 39 3 2 76 278 369 5.26e-14 65.9
Msa1121350 MtrunA17_Chr3g0092641 42.697 89 46 2 4 87 308 396 5.87e-14 65.9
Msa1121350 MtrunA17_Chr6g0454321 37.079 89 51 2 4 87 308 396 6.16e-14 65.9
Msa1121350 MtrunA17_Chr4g0066151 41.667 84 35 4 6 75 289 372 6.24e-14 65.5
Msa1121350 MtrunA17_Chr7g0243811 34.783 92 52 2 1 84 259 350 6.83e-14 65.5
Msa1121350 MtrunA17_Chr6g0462531 40.000 85 42 3 1 76 284 368 6.84e-14 65.5
Msa1121350 MtrunA17_Chr7g0232311 33.613 119 51 4 2 92 274 392 7.04e-14 65.5
Msa1121350 MtrunA17_Chr4g0032331 39.394 99 52 2 1 91 276 374 7.28e-14 65.5
Msa1121350 MtrunA17_Chr7g0243861 35.870 92 51 1 1 84 298 389 7.75e-14 65.5
Msa1121350 MtrunA17_Chr1g0153451 34.653 101 48 3 2 84 275 375 7.77e-14 65.5
Msa1121350 MtrunA17_Chr8g0339631 36.634 101 45 3 3 84 281 381 7.94e-14 65.5
Msa1121350 MtrunA17_Chr2g0307961 41.558 77 43 2 4 78 300 376 8.00e-14 65.5
Msa1121350 MtrunA17_Chr5g0426431 40.217 92 50 2 1 87 305 396 8.10e-14 65.5
Msa1121350 MtrunA17_Chr3g0079711 40.449 89 33 4 8 76 3 91 8.36e-14 63.5
Msa1121350 MtrunA17_Chr6g0454351 42.857 77 39 2 4 75 295 371 8.81e-14 65.1
Msa1121350 MtrunA17_Chr8g0336691 35.294 102 47 3 2 84 1190 1291 8.90e-14 65.5
Msa1121350 MtrunA17_Chr8g0336691 34.314 102 48 3 2 84 284 385 1.77e-12 61.6
Msa1121350 MtrunA17_Chr8g0336331 39.326 89 49 2 4 87 307 395 8.93e-14 65.1
Msa1121350 MtrunA17_Chr3g0118771 31.183 93 58 2 1 87 315 407 9.97e-14 65.1
Msa1121350 MtrunA17_Chr3g0078841 36.957 92 53 2 1 87 305 396 1.26e-13 64.7
Msa1121350 MtrunA17_Chr7g0232391 33.000 100 50 3 2 84 278 377 1.31e-13 64.7
Msa1121350 MtrunA17_Chr7g0243871 34.783 92 52 2 1 84 283 374 1.33e-13 64.7
Msa1121350 MtrunA17_Chr7g0232331 32.673 101 51 3 1 84 277 377 1.39e-13 64.7
Msa1121350 MtrunA17_Chr3g0118671 33.333 90 54 2 4 87 303 392 1.40e-13 64.7
Msa1121350 MtrunA17_Chr7g0218821 37.662 77 46 1 1 75 280 356 1.40e-13 64.7
Msa1121350 MtrunA17_Chr5g0432731 43.836 73 39 2 3 75 125 195 1.44e-13 64.3
Msa1121350 MtrunA17_Chr4g0066261 40.964 83 36 4 6 75 292 374 1.44e-13 64.7
Msa1121350 MtrunA17_Chr4g0044521 39.535 86 42 3 1 76 276 361 1.46e-13 64.7
Msa1121350 MtrunA17_Chr6g0454151 37.079 89 51 2 4 87 308 396 1.63e-13 64.3
Msa1121350 MtrunA17_Chr5g0442511 31.633 98 50 2 4 84 302 399 1.72e-13 64.3
Msa1121350 MtrunA17_Chr6g0462551 36.471 85 45 3 1 76 284 368 1.75e-13 64.3
Msa1121350 MtrunA17_Chr7g0218791 40.449 89 51 1 1 87 277 365 1.93e-13 64.3
Msa1121350 MtrunA17_Chr4g0066281 36.364 88 46 2 7 84 281 368 1.93e-13 64.3
Msa1121350 MtrunA17_Chr5g0423981 36.957 92 53 2 1 87 272 363 1.95e-13 64.3
Msa1121350 MtrunA17_Chr5g0431361 41.892 74 39 2 3 76 282 351 1.97e-13 64.3
Msa1121350 MtrunA17_Chr6g0454341 36.957 92 53 1 1 87 305 396 1.99e-13 64.3
Msa1121350 MtrunA17_Chr6g0462541 37.647 85 44 3 1 76 283 367 2.06e-13 64.3
Msa1121350 MtrunA17_Chr6g0462571 37.647 85 44 3 1 76 281 365 2.08e-13 64.3
Msa1121350 MtrunA17_Chr6g0454401 36.957 92 53 2 1 87 308 399 2.44e-13 63.9
Msa1121350 MtrunA17_Chr8g0334831 32.727 110 47 3 2 84 275 384 2.44e-13 63.9
Msa1121350 MtrunA17_Chr6g0454221 35.870 92 54 2 1 87 58 149 2.53e-13 63.5
Msa1121350 MtrunA17_Chr6g0449931 39.175 97 39 3 2 78 277 373 2.66e-13 63.9
Msa1121350 MtrunA17_Chr3g0112761 40.426 94 49 2 1 87 274 367 2.67e-13 63.9
Msa1121350 MtrunA17_Chr8g0355011 33.696 92 56 2 1 87 189 280 2.73e-13 63.5
Msa1121350 MtrunA17_Chr8g0374901 35.955 89 43 4 1 75 283 371 2.94e-13 63.9
Msa1121350 MtrunA17_Chr7g0232351 32.000 100 51 3 2 84 277 376 3.48e-13 63.5
Msa1121350 MtrunA17_Chr4g0050341 37.634 93 52 2 1 87 317 409 3.94e-13 63.5
Msa1121350 MtrunA17_Chr6g0454211 35.870 92 54 2 1 87 594 685 4.32e-13 63.2
Msa1121350 MtrunA17_Chr6g0453951 35.955 89 52 2 4 87 357 445 4.64e-13 63.2
Msa1121350 MtrunA17_Chr8g0369341 39.759 83 40 4 4 76 292 374 4.91e-13 63.2
Msa1121350 MtrunA17_Chr4g0066221 41.860 86 36 4 4 75 288 373 6.04e-13 62.8
Msa1121350 MtrunA17_Chr8g0374971 36.047 86 41 3 4 75 288 373 6.16e-13 62.8
Msa1121350 MtrunA17_Chr3g0118661 35.955 89 52 2 1 84 307 395 6.33e-13 62.8
Msa1121350 MtrunA17_Chr8g0339461 36.538 104 45 3 2 84 280 383 6.64e-13 62.8
Msa1121350 MtrunA17_Chr6g0462521 38.824 85 43 3 1 76 270 354 6.74e-13 62.8
Msa1121350 MtrunA17_Chr8g0334861 36.170 94 41 3 2 76 274 367 7.45e-13 62.8
Msa1121350 MtrunA17_Chr8g0339451 35.577 104 46 3 2 84 272 375 7.74e-13 62.4
Msa1121350 MtrunA17_Chr3g0118711 32.222 90 55 2 4 87 310 399 8.16e-13 62.4
Msa1121350 MtrunA17_Chr6g0454141 35.955 89 52 2 4 87 310 398 8.40e-13 62.4
Msa1121350 MtrunA17_Chr8g0339441 35.577 104 46 3 2 84 238 341 9.77e-13 62.4
Msa1121350 MtrunA17_Chr6g0454331 35.870 92 54 2 1 87 306 397 1.03e-12 62.0
Msa1121350 MtrunA17_Chr7g0243931 37.647 85 45 3 8 84 308 392 1.09e-12 62.0
Msa1121350 MtrunA17_Chr6g0454201 34.831 89 53 2 4 87 311 399 1.18e-12 62.0
Msa1121350 MtrunA17_Chr6g0454121 35.870 92 54 2 1 87 306 397 1.26e-12 62.0
Msa1121350 MtrunA17_Chr4g0014061 37.349 83 44 2 1 75 283 365 1.26e-12 62.0
Msa1121350 MtrunA17_Chr6g0454291 35.955 89 52 2 4 87 307 395 1.69e-12 61.6
Msa1121350 MtrunA17_Chr8g0374941 36.364 88 42 4 1 74 1222 1309 1.72e-12 61.6
Msa1121350 MtrunA17_Chr8g0374941 36.364 88 42 4 1 74 284 371 2.11e-12 61.2
Msa1121350 MtrunA17_Chr8g0374941 32.584 89 46 3 1 75 749 837 6.80e-12 60.1
Msa1121350 MtrunA17_Chr4g0042891 42.169 83 37 3 3 74 286 368 1.72e-12 61.6
Msa1121350 MtrunA17_Chr1g0153461 32.673 101 50 3 2 84 24 124 1.85e-12 60.5
Msa1121350 MtrunA17_Chr6g0454261 34.783 92 55 2 1 87 307 398 1.94e-12 61.2
Msa1121350 MtrunA17_Chr6g0454271 33.696 92 56 2 1 87 114 205 2.25e-12 60.8
Msa1121350 MtrunA17_Chr6g0454361 35.955 89 52 2 4 87 311 399 2.41e-12 61.2
Msa1121350 MtrunA17_Chr8g0339601 34.615 104 47 3 2 84 139 242 2.67e-12 60.8
Msa1121350 MtrunA17_Chr6g0454131 37.079 89 51 1 4 87 309 397 2.73e-12 60.8
Msa1121350 MtrunA17_Chr6g0454251 34.831 89 53 2 4 87 307 395 2.88e-12 60.8
Msa1121350 MtrunA17_Chr6g0452771 37.662 77 43 2 4 75 310 386 2.98e-12 60.8
Msa1121350 MtrunA17_Chr6g0454191 35.955 89 52 2 4 87 311 399 2.98e-12 60.8
Msa1121350 MtrunA17_Chr7g0243711 36.709 79 42 2 8 78 134 212 3.69e-12 60.5
Msa1121350 MtrunA17_Chr5g0419771 36.782 87 44 3 1 76 317 403 6.48e-12 60.1
Msa1121350 MtrunA17_Chr2g0329111 32.653 98 52 4 1 84 286 383 6.64e-12 60.1
Msa1121350 MtrunA17_Chr7g0243851 34.118 85 48 2 8 84 260 344 6.78e-12 59.7
Msa1121350 MtrunA17_Chr6g0482061 38.710 93 46 3 3 84 300 392 6.96e-12 59.7
Msa1121350 MtrunA17_Chr7g0275511 40.625 96 38 4 2 78 286 381 8.62e-12 59.7
Msa1121350 MtrunA17_Chr4g0066131 31.579 95 51 2 4 84 282 376 8.96e-12 59.7
Msa1121350 MtrunA17_Chr6g0454231 38.889 90 50 3 1 87 49 136 9.13e-12 59.3
Msa1121350 MtrunA17_Chr7g0275681 38.272 81 45 2 1 76 293 373 1.08e-11 59.3
Msa1121350 MtrunA17_Chr8g0359821 35.955 89 52 1 4 87 308 396 1.14e-11 59.3
Msa1121350 MtrunA17_Chr5g0440421 33.684 95 50 4 3 84 306 400 1.22e-11 59.3
Msa1121350 MtrunA17_Chr4g0042931 39.286 84 39 2 3 74 283 366 1.26e-11 58.9
Msa1121350 MtrunA17_Chr5g0420801 37.349 83 42 4 4 76 282 364 1.32e-11 58.9
Msa1121350 MtrunA17_Chr8g0336251 36.782 87 48 3 4 83 291 377 1.50e-11 58.9
Msa1121350 MtrunA17_Chr7g0241781 31.707 82 56 0 3 84 262 343 1.75e-11 58.5
Msa1121350 MtrunA17_Chr8g0336321 36.782 87 48 3 4 83 291 377 1.89e-11 58.5
Msa1121350 MtrunA17_Chr7g0265111 36.782 87 44 3 1 76 322 408 2.07e-11 58.5
Msa1121350 MtrunA17_Chr5g0431421 39.474 76 42 2 3 78 277 348 2.43e-11 58.2
Msa1121350 MtrunA17_Chr7g0241801 32.099 81 55 0 4 84 263 343 2.44e-11 58.2
Msa1121350 MtrunA17_Chr6g0454171 41.250 80 42 2 13 87 149 228 2.48e-11 58.2
Msa1121350 MtrunA17_Chr5g0409591 33.333 90 39 3 7 75 300 389 2.54e-11 58.2
Msa1121350 MtrunA17_Chr8g0367221 31.868 91 54 2 2 84 289 379 2.95e-11 58.2
Msa1121350 MtrunA17_Chr8g0367221 31.868 91 54 3 2 84 1076 1166 6.18e-11 57.4
Msa1121350 MtrunA17_Chr8g0367221 31.868 91 54 2 2 84 1566 1656 8.44e-11 57.0
Msa1121350 MtrunA17_Chr8g0347281 36.170 94 41 3 4 78 285 378 3.40e-11 57.8
Msa1121350 MtrunA17_Chr2g0294941 39.560 91 42 4 7 84 191 281 3.51e-11 57.8
Msa1121350 MtrunA17_Chr7g0218311 35.714 84 41 3 4 74 292 375 4.03e-11 57.8
Msa1121350 MtrunA17_Chr8g0334851 36.634 101 46 4 2 84 281 381 4.13e-11 57.8
Msa1121350 MtrunA17_Chr5g0431401 40.541 74 40 2 3 76 277 346 4.19e-11 57.8
Msa1121350 MtrunA17_Chr1g0179901 37.931 87 43 3 1 76 298 384 5.30e-11 57.4
Msa1121350 MtrunA17_Chr7g0243911 35.294 85 47 2 8 84 48 132 5.35e-11 56.6
Msa1121350 MtrunA17_Chr2g0278741 41.333 75 40 3 4 74 376 450 5.40e-11 57.4
Msa1121350 MtrunA17_Chr5g0416821 33.684 95 51 3 2 84 296 390 5.71e-11 57.4
Msa1121350 MtrunA17_Chr4g0069611 36.364 77 45 3 2 74 287 363 5.91e-11 57.0
Msa1121350 MtrunA17_Chr2g0278721 41.333 75 40 3 4 74 294 368 7.36e-11 57.0
Msa1121350 MtrunA17_Chr6g0466831 39.241 79 44 3 2 76 290 368 7.69e-11 57.0
Msa1121350 MtrunA17_Chr6g0463851 32.955 88 44 3 3 75 289 376 7.81e-11 57.0
Msa1121350 MtrunA17_Chr5g0431371 41.892 74 39 2 3 76 277 346 9.22e-11 56.6
Msa1121350 MtrunA17_Chr2g0294931 35.294 85 42 3 4 75 290 374 9.41e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1121350 AT2G31750.1 54.545 77 34 1 1 76 280 356 1.42e-24 95.9
Msa1121350 AT2G31750.2 54.545 77 34 1 1 76 280 356 1.91e-24 95.9
Msa1121350 AT1G05680.1 49.412 85 42 1 1 84 280 364 2.37e-24 95.5
Msa1121350 AT2G31790.1 53.165 79 34 1 1 76 282 360 8.36e-24 94.0
Msa1121350 AT1G05675.1 49.412 85 42 1 1 84 280 364 1.64e-23 92.8
Msa1121350 AT2G43840.2 47.674 86 41 2 1 84 274 357 6.70e-22 88.6
Msa1121350 AT2G43840.1 47.674 86 41 2 1 84 274 357 7.86e-22 88.2
Msa1121350 AT2G43820.1 48.837 86 40 2 1 84 274 357 9.31e-22 88.2
Msa1121350 AT1G24100.1 51.220 82 39 1 4 84 288 369 8.27e-21 85.5
Msa1121350 AT1G07250.1 44.444 90 43 2 2 84 293 382 7.07e-20 82.8
Msa1121350 AT4G15490.1 44.086 93 43 2 1 87 290 379 4.09e-19 80.9
Msa1121350 AT4G15480.1 42.105 95 47 2 1 87 297 391 4.10e-19 80.9
Msa1121350 AT2G29730.1 42.529 87 47 2 1 84 285 371 7.93e-19 80.1
Msa1121350 AT2G29710.1 42.045 88 47 2 1 84 284 371 1.22e-18 79.3
Msa1121350 AT1G07260.1 45.238 84 39 2 8 84 298 381 2.14e-18 78.6
Msa1121350 AT2G29740.1 42.857 91 45 2 1 84 294 384 5.62e-18 77.4
Msa1121350 AT1G07240.1 38.462 91 49 2 1 84 291 381 2.16e-17 75.9
Msa1121350 AT5G17050.1 41.975 81 46 1 8 87 294 374 9.01e-17 73.9
Msa1121350 AT2G23260.1 45.205 73 38 1 6 76 284 356 1.17e-16 73.9
Msa1121350 AT3G21560.1 38.710 93 48 2 1 87 296 385 1.84e-16 73.2
Msa1121350 AT2G30140.2 48.649 74 35 2 3 76 280 350 2.58e-16 72.8
Msa1121350 AT2G30140.1 48.649 74 35 2 3 76 281 351 2.74e-16 72.8
Msa1121350 AT1G05530.1 42.268 97 43 3 1 84 271 367 2.99e-16 72.8
Msa1121350 AT3G21750.1 37.624 101 46 2 1 84 274 374 3.09e-16 72.8
Msa1121350 AT1G05560.2 39.175 97 46 2 1 84 318 414 4.38e-16 72.0
Msa1121350 AT2G29750.1 37.363 91 50 2 1 84 294 384 4.41e-16 72.0
Msa1121350 AT1G05560.1 39.175 97 46 2 1 84 268 364 4.64e-16 72.0
Msa1121350 AT3G21790.1 39.394 99 45 2 1 84 285 383 4.84e-16 72.0
Msa1121350 AT4G14090.1 46.512 86 42 2 3 84 281 366 7.21e-16 71.6
Msa1121350 AT2G15490.3 46.914 81 36 3 3 76 297 377 7.68e-16 71.6
Msa1121350 AT5G05870.1 42.553 94 47 2 1 87 280 373 8.39e-16 71.2
Msa1121350 AT1G78270.1 43.011 93 47 2 1 87 306 398 8.50e-16 71.2
Msa1121350 AT2G28080.1 38.043 92 51 3 2 87 299 390 1.61e-15 70.5
Msa1121350 AT3G11340.1 40.816 98 51 2 1 91 270 367 1.96e-15 70.5
Msa1121350 AT2G15490.1 45.238 84 36 3 3 76 297 380 2.00e-15 70.5
Msa1121350 AT4G15500.1 39.785 93 47 2 1 87 286 375 2.35e-15 70.1
Msa1121350 AT1G30530.1 41.772 79 45 1 7 84 287 365 2.56e-15 70.1
Msa1121350 AT3G22250.1 39.241 79 44 1 1 75 294 372 3.22e-15 69.7
Msa1121350 AT5G49690.1 42.667 75 39 3 2 74 285 357 3.35e-15 69.7
Msa1121350 AT2G30150.2 47.297 74 36 2 3 76 282 352 3.51e-15 69.7
Msa1121350 AT1G22400.1 40.449 89 48 2 4 87 311 399 3.81e-15 69.3
Msa1121350 AT2G30150.1 47.297 74 36 2 3 76 266 336 3.83e-15 69.3
Msa1121350 AT4G34135.1 40.506 79 42 3 1 74 299 377 3.84e-15 69.3
Msa1121350 AT4G15280.2 38.384 99 46 2 1 84 313 411 4.31e-15 69.3
Msa1121350 AT4G15260.1 39.394 99 45 2 1 84 161 259 5.05e-15 68.9
Msa1121350 AT3G16520.2 43.902 82 35 3 4 74 283 364 5.10e-15 68.9
Msa1121350 AT3G16520.1 43.902 82 35 3 4 74 283 364 5.12e-15 68.9
Msa1121350 AT4G15280.1 38.384 99 46 2 1 84 281 379 5.55e-15 68.9
Msa1121350 AT5G59580.1 39.362 94 50 3 1 87 273 366 6.29e-15 68.9
Msa1121350 AT3G21800.1 36.364 99 48 2 1 84 282 380 6.43e-15 68.9
Msa1121350 AT3G16520.3 41.379 87 40 3 1 76 280 366 7.18e-15 68.6
Msa1121350 AT5G05880.1 41.489 94 48 2 1 87 274 367 9.29e-15 68.6
Msa1121350 AT1G22380.1 39.326 89 49 2 4 87 310 398 9.62e-15 68.6
Msa1121350 AT3G46660.1 37.895 95 52 2 1 88 281 375 1.01e-14 68.2
Msa1121350 AT5G05900.1 39.080 87 53 0 1 87 280 366 1.24e-14 68.2
Msa1121350 AT4G34138.1 44.444 81 38 3 1 74 298 378 1.31e-14 68.2
Msa1121350 AT2G23250.1 41.667 72 40 1 6 75 266 337 1.43e-14 67.8
Msa1121350 AT4G36770.1 42.268 97 36 4 2 78 274 370 1.76e-14 67.8
Msa1121350 AT3G21760.1 37.374 99 47 2 1 84 288 386 2.38e-14 67.4
Msa1121350 AT5G17030.1 38.272 81 49 1 8 87 293 373 2.38e-14 67.4
Msa1121350 AT2G15480.2 43.038 79 38 3 3 74 300 378 2.63e-14 67.0
Msa1121350 AT5G59590.1 40.426 94 49 3 1 87 275 368 3.33e-14 66.6
Msa1121350 AT1G22360.2 39.326 89 49 2 4 87 307 395 3.67e-14 66.6
Msa1121350 AT1G22360.1 39.326 89 49 2 4 87 307 395 3.68e-14 66.6
Msa1121350 AT1G22340.1 36.559 93 53 2 1 87 307 399 4.03e-14 66.6
Msa1121350 AT3G55700.1 39.583 96 51 2 1 89 276 371 4.15e-14 66.6
Msa1121350 AT2G15480.1 42.500 80 39 3 3 75 300 379 4.31e-14 66.6
Msa1121350 AT5G17040.2 41.026 78 45 1 12 88 179 256 4.57e-14 66.2
Msa1121350 AT5G17040.1 41.558 77 44 1 12 87 281 357 7.91e-14 65.9
Msa1121350 AT2G36970.1 38.043 92 51 2 2 87 296 387 1.10e-13 65.5
Msa1121350 AT3G46720.1 38.636 88 52 2 1 87 273 359 1.89e-13 64.7
Msa1121350 AT3G46680.1 34.951 103 55 3 1 96 275 372 2.15e-13 64.7
Msa1121350 AT3G46670.1 36.842 95 53 2 1 88 274 368 2.15e-13 64.7
Msa1121350 AT1G01420.1 36.842 95 42 3 2 78 279 373 3.14e-13 63.9
Msa1121350 AT1G01420.2 36.842 95 42 3 2 78 282 376 3.14e-13 63.9
Msa1121350 AT3G46700.1 35.106 94 54 2 1 87 270 363 3.49e-13 63.9
Msa1121350 AT4G15550.1 38.889 90 44 2 1 79 288 377 4.41e-13 63.5
Msa1121350 AT1G01390.2 36.842 95 42 3 2 78 296 390 4.48e-13 63.5
Msa1121350 AT1G01390.1 36.842 95 42 3 2 78 279 373 5.16e-13 63.5
Msa1121350 AT3G02100.1 41.667 84 43 1 4 87 299 376 5.89e-13 63.2
Msa1121350 AT5G65550.1 34.884 86 52 3 3 84 289 374 6.50e-13 63.2
Msa1121350 AT1G22370.2 37.079 89 51 2 4 87 305 393 1.22e-12 62.4
Msa1121350 AT4G34131.1 40.000 80 42 3 1 74 298 377 1.52e-12 62.0
Msa1121350 AT4G01070.1 40.000 90 36 3 7 78 284 373 1.65e-12 62.0
Msa1121350 AT2G36780.1 38.961 77 39 3 8 76 305 381 1.87e-12 62.0
Msa1121350 AT3G21780.1 36.364 99 48 2 1 84 276 374 2.02e-12 61.6
Msa1121350 AT1G22370.1 37.079 89 51 2 4 87 135 223 2.17e-12 61.2
Msa1121350 AT1G10400.1 42.105 76 38 3 2 74 291 363 2.28e-12 61.6
Msa1121350 AT3G46690.1 35.484 93 53 2 2 87 276 368 4.72e-12 60.8
Msa1121350 AT5G12890.1 34.694 98 50 4 1 84 293 390 5.15e-12 60.5
Msa1121350 AT5G26310.1 34.343 99 43 4 2 78 274 372 5.45e-12 60.5
Msa1121350 AT5G38040.1 35.484 93 50 2 4 88 279 369 6.20e-12 60.5
Msa1121350 AT3G53150.1 35.484 93 51 2 1 84 308 400 6.42e-12 60.5
Msa1121350 AT5G38010.1 35.165 91 53 1 4 88 283 373 7.69e-12 60.1
Msa1121350 AT5G14860.1 40.260 77 43 3 2 75 298 374 7.99e-12 60.1
Msa1121350 AT3G55710.1 38.947 95 51 2 1 88 280 374 1.04e-11 59.7
Msa1121350 AT2G16890.2 37.333 75 45 2 2 74 291 365 1.09e-11 59.7
Msa1121350 AT1G73880.1 39.744 78 40 3 4 74 293 370 1.14e-11 59.7
Msa1121350 AT2G16890.3 37.333 75 45 2 2 74 291 365 1.19e-11 59.7
Msa1121350 AT1G51210.1 38.667 75 41 3 4 76 291 362 1.40e-11 59.3
Msa1121350 AT3G50740.1 34.343 99 43 3 2 78 279 377 1.66e-11 59.3
Msa1121350 AT3G46650.1 34.043 94 55 2 1 87 258 351 3.05e-11 58.5
Msa1121350 AT5G03490.1 40.789 76 40 3 3 76 294 366 3.17e-11 58.5
Msa1121350 AT5G66690.1 35.052 97 41 4 4 78 276 372 4.70e-11 57.8

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGTGGAAGTTACTTTCTA+TGG 0.266459 7_3:-71424086 Msa1121350:CDS
CATTTGGAAGCTTTGAGTAT+AGG 0.357644 7_3:-71423915 Msa1121350:CDS
CATGAGCTAGAACCTCTAAC+TGG 0.362003 7_3:+71423980 None:intergenic
TAAAGTGGCATTGCAACAAT+TGG 0.372127 7_3:+71423888 None:intergenic
GTTCTAGCTCATGAAGCAAT+AGG 0.390537 7_3:-71423970 Msa1121350:CDS
TGTGGTTGGAATTCTACATT+TGG 0.431437 7_3:-71423931 Msa1121350:CDS
TTTGAAAAGAAATTAGAGAA+TGG 0.467596 7_3:-71424024 Msa1121350:CDS
ACTCAATGAGGAACAAATAG+AGG 0.483111 7_3:-71424130 Msa1121350:CDS
GCACATTGTTTGAAAGATTG+TGG 0.490834 7_3:-71424102 Msa1121350:CDS
TTCTAGCTCATGAAGCAATA+GGG 0.491607 7_3:-71423969 Msa1121350:CDS
GAAACTTTGCATCAATGCCT+TGG 0.505772 7_3:+71423859 None:intergenic
TTGTGGAAGTTACTTTCTAT+GGG 0.521178 7_3:-71424085 Msa1121350:CDS
AAAGTGGCATTGCAACAATT+GGG 0.549651 7_3:+71423889 None:intergenic
ATGAGCTAGAACCTCTAACT+GGG 0.569040 7_3:+71423981 None:intergenic
TGGGAGCTTAGTCTCTTCAG+AGG 0.586731 7_3:+71424053 None:intergenic
ATGCCTTGGTCTGAGTAAAG+TGG 0.604432 7_3:+71423873 None:intergenic
ATGCCACTTTACTCAGACCA+AGG 0.625688 7_3:-71423876 Msa1121350:CDS
TGAGCTAGAACCTCTAACTG+GGG 0.637465 7_3:+71423982 None:intergenic
AAGTATGGCATCACTCAATG+AGG 0.672707 7_3:-71424142 None:intergenic
GAGCTAGAACCTCTAACTGG+GGG 0.700576 7_3:+71423983 None:intergenic
AGAATGGCTTAGTTGTGACA+TGG 0.715378 7_3:-71424008 Msa1121350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCTTTTCAAAGTCTTTT+GGG + chr7_3:71423977-71423996 None:intergenic 20.0%
!!! AATTTCTTTTCAAAGTCTTT+TGG + chr7_3:71423978-71423997 None:intergenic 20.0%
!! TTTGAAAAGAAATTAGAGAA+TGG - chr7_3:71423984-71424003 Msa1121350:CDS 20.0%
! ATTGTGGAAGTTACTTTCTA+TGG - chr7_3:71423922-71423941 Msa1121350:CDS 30.0%
! TTGTGGAAGTTACTTTCTAT+GGG - chr7_3:71423923-71423942 Msa1121350:CDS 30.0%
ACTCAATGAGGAACAAATAG+AGG - chr7_3:71423878-71423897 Msa1121350:CDS 35.0%
GCACATTGTTTGAAAGATTG+TGG - chr7_3:71423906-71423925 Msa1121350:CDS 35.0%
!! TTCTAGCTCATGAAGCAATA+GGG - chr7_3:71424039-71424058 Msa1121350:CDS 35.0%
! GTTTTGTAACACATTGTGGT+TGG - chr7_3:71424063-71424082 Msa1121350:CDS 35.0%
TGTGGTTGGAATTCTACATT+TGG - chr7_3:71424077-71424096 Msa1121350:CDS 35.0%
!! CATTTGGAAGCTTTGAGTAT+AGG - chr7_3:71424093-71424112 Msa1121350:CDS 35.0%
AAAGTGGCATTGCAACAATT+GGG + chr7_3:71424122-71424141 None:intergenic 35.0%
TAAAGTGGCATTGCAACAAT+TGG + chr7_3:71424123-71424142 None:intergenic 35.0%
AGAATGGCTTAGTTGTGACA+TGG - chr7_3:71424000-71424019 Msa1121350:CDS 40.0%
ATGAGCTAGAACCTCTAACT+GGG + chr7_3:71424030-71424049 None:intergenic 40.0%
!! GTTCTAGCTCATGAAGCAAT+AGG - chr7_3:71424038-71424057 Msa1121350:CDS 40.0%
! GGGTGTTTTGTAACACATTG+TGG - chr7_3:71424059-71424078 Msa1121350:CDS 40.0%
TGAGCTAGAACCTCTAACTG+GGG + chr7_3:71424029-71424048 None:intergenic 45.0%
CATGAGCTAGAACCTCTAAC+TGG + chr7_3:71424031-71424050 None:intergenic 45.0%
!! ATGCCACTTTACTCAGACCA+AGG - chr7_3:71424132-71424151 Msa1121350:CDS 45.0%
ATGCCTTGGTCTGAGTAAAG+TGG + chr7_3:71424138-71424157 None:intergenic 45.0%
TGGGAGCTTAGTCTCTTCAG+AGG + chr7_3:71423958-71423977 None:intergenic 50.0%
GAGCTAGAACCTCTAACTGG+GGG + chr7_3:71424028-71424047 None:intergenic 50.0%
GACATGGTGCCCCCAGTTAG+AGG - chr7_3:71424016-71424035 Msa1121350:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 71423870 71424160 71423870 ID=Msa1121350;Name=Msa1121350
chr7_3 mRNA 71423870 71424160 71423870 ID=Msa1121350-mRNA-1;Parent=Msa1121350;Name=Msa1121350-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|96
chr7_3 exon 71423870 71424160 71423870 ID=Msa1121350-mRNA-1:exon:18282;Parent=Msa1121350-mRNA-1
chr7_3 CDS 71423870 71424160 71423870 ID=Msa1121350-mRNA-1:cds;Parent=Msa1121350-mRNA-1
Gene Sequence

>Msa1121350

ATGGCATCACTCAATGAGGAACAAATAGAGGAAGTAGCACATTGTTTGAAAGATTGTGGAAGTTACTTTCTATGGGTAGTGAAAACCTCTGAAGAGACTAAGCTCCCAAAAGACTTTGAAAAGAAATTAGAGAATGGCTTAGTTGTGACATGGTGCCCCCAGTTAGAGGTTCTAGCTCATGAAGCAATAGGGTGTTTTGTAACACATTGTGGTTGGAATTCTACATTTGGAAGCTTTGAGTATAGGAGTCCCAATTGTTGCAATGCCACTTTACTCAGACCAAGGCATTGA
Protein sequence

>Msa1121350

MASLNEEQIEEVAHCLKDCGSYFLWVVKTSEETKLPKDFEKKLENGLVVTWCPQLEVLAHEAIGCFVTHCGWNSTFGSFEYRSPNCCNATLLRPRH*