AlfalfaGEDB | Msa1121420

M. sativa cultivar zhongmu-4 / Msa1121420

KEGG: K08738
GO: GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005758 GO:0005773 GO:0005774 GO:0005829 GO:0006091 GO:0006119 GO:0006122 GO:0006123 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008283 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009987 GO:0010336 GO:0015980 GO:0016020 GO:0016310 GO:0017144 GO:0019637 GO:0019646 GO:0019693 GO:0022900 GO:0022904 GO:0031090 GO:0031967 GO:0031970 GO:0031974 GO:0031975 GO:0034641 GO:0042592 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0048878 GO:0050801 GO:0055081 GO:0055086 GO:0055088 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072521 GO:0098588 GO:0098805 GO:1901135 GO:1901360 GO:1901564
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1121420	CAN60482.1	97.436	39	1	0	1	39	1	39	2.61e-19	85.5

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1121420	sp\|Q0DI31\|CYC_ORYSJ	92.308	39	3	0	1	39	1	39	1.85e-20	80.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1121420	A5AQD0	97.436	39	1	0	1	39	1	39	1.25e-19	85.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0358820	Msa1121420	0.814030	1.895674e-51	-8.615850e-47
Msa1121380	Msa1121420	0.817671	2.922080e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1121420	MtrunA17_Chr7g0249831	94.872	39	2	0	1	39	1	39	6.41e-23	84.3
Msa1121420	MtrunA17_Chr5g0396151	76.923	39	9	0	1	39	1	39	2.52e-17	70.1

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1121420	AT4G10040.1	89.744	39	4	1	39	1	39	2.76e-21	80.5
Msa1121420	AT1G22840.2	84.615	39	6	1	39	1	39	2.32e-19	75.5
Msa1121420	AT1G22840.1	84.615	39	6	1	39	1	39	2.87e-19	75.5

Find 24 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCGCCGGCGGCGGGGTTTC+CGG	0.164134	7_3:-71482449	None:intergenic
TCGCCGGCGGCGGGGTTTCC+GGG	0.240370	7_3:-71482448	None:intergenic
AAGATCTTCTCGCCGGCGGC+GGG	0.329325	7_3:-71482457	None:intergenic
TGAGATGAGAAGAGGAGAGA+TGG	0.384852	7_3:-71482989	None:intergenic
TGTGTTTGCGGCTGTGTATA+TGG	0.393961	7_3:-71482900	None:intergenic
GAGATGAGAAGAGGAGAGAT+GGG	0.405757	7_3:-71482988	None:intergenic
GAAGATCTTCTCGCCGGCGG+CGG	0.418146	7_3:-71482458	None:intergenic
AGATTAGCATACCTTGTTTG+TGG	0.420990	7_3:-71482525	None:intergenic
GATGATGAGATGAGAAGAGA+TGG	0.489585	7_3:-71482951	None:intergenic
GAGAAGAGGAGAGATGGGTT+CGG	0.495393	7_3:-71482983	None:intergenic
GTGGCCGGCGCCTTTGTCGA+CGG	0.510944	7_3:-71482506	None:intergenic
TAGCATACCTTGTTTGTGGC+CGG	0.513390	7_3:-71482521	None:intergenic
GGTGTGGCACTGAGCGCACT+TGG	0.520968	7_3:-71482485	None:intergenic
TCTTTCGATGAAGCTCCTCC+CGG	0.532703	7_3:+71482430	Msa1121420:CDS
AAAGATGATGAGATGAGAAG+AGG	0.543490	7_3:-71482997	None:intergenic
TGATGAAGAAGTTGTGTTTG+CGG	0.545175	7_3:-71482912	None:intergenic
CCTCCCGGAAACCCCGCCGC+CGG	0.551439	7_3:+71482445	Msa1121420:CDS
AGATCTTCTCGCCGGCGGCG+GGG	0.553653	7_3:-71482456	None:intergenic
CAGTGCCACACCGTCGACAA+AGG	0.583975	7_3:+71482496	Msa1121420:CDS
TTTGAAGATCTTCTCGCCGG+CGG	0.594334	7_3:-71482461	None:intergenic
CACACCGTCGACAAAGGCGC+CGG	0.616193	7_3:+71482502	Msa1121420:CDS
AAAGGCGCCGGCCACAAACA+AGG	0.616957	7_3:+71482514	Msa1121420:CDS
CGGCGCCTTTGTCGACGGTG+TGG	0.644445	7_3:-71482501	None:intergenic
CCGGCGGCGGGGTTTCCGGG+AGG	0.653806	7_3:-71482445	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATATAAACTTAACAGCGATT+AGG	-	chr7_3:71482644-71482663	None:intergenic	25.0%
!!	TTCACTTAAAACCGTTTTAA+AGG	+	chr7_3:71482829-71482848	Msa1121420:intron	25.0%
!!	TGCAAAAATGACCTTTAAAA+CGG	-	chr7_3:71482843-71482862	None:intergenic	25.0%
!!	TGTAGATCTGAGAGTTTTAT+GGG	-	chr7_3:71482561-71482580	None:intergenic	30.0%
	AAAACGAAAGAAAACAACAC+AGG	-	chr7_3:71482587-71482606	None:intergenic	30.0%
	TGATTGTTCAAGATCTTGTT+AGG	+	chr7_3:71482680-71482699	Msa1121420:intron	30.0%
	GATTGTTCAAGATCTTGTTA+GGG	+	chr7_3:71482681-71482700	Msa1121420:intron	30.0%
	ATGGAGAAGAGTAATAGTTA+GGG	-	chr7_3:71482875-71482894	None:intergenic	30.0%
!	AGATTAGCATACCTTGTTTG+TGG	-	chr7_3:71482528-71482547	None:intergenic	35.0%
!!	GTAGATCTGAGAGTTTTATG+GGG	-	chr7_3:71482560-71482579	None:intergenic	35.0%
!	GTGTAGATCTGAGAGTTTTA+TGG	-	chr7_3:71482562-71482581	None:intergenic	35.0%
	GATGGAGAAGAGTAATAGTT+AGG	-	chr7_3:71482876-71482895	None:intergenic	35.0%
	TGATGAAGAAGTTGTGTTTG+CGG	-	chr7_3:71482915-71482934	None:intergenic	35.0%
	AAAGATGATGAGATGAGAAG+AGG	-	chr7_3:71483000-71483019	None:intergenic	35.0%
	GATGATGAGATGAGAAGAGA+TGG	-	chr7_3:71482954-71482973	None:intergenic	40.0%
!!!	GGTTTTGAAGATCTTCTCGC+CGG	-	chr7_3:71482467-71482486	None:intergenic	45.0%
!	TAGCATACCTTGTTTGTGGC+CGG	-	chr7_3:71482524-71482543	None:intergenic	45.0%
!!	GGCTGTGTATATGGTTCTGA+TGG	-	chr7_3:71482894-71482913	None:intergenic	45.0%
	TGTGTTTGCGGCTGTGTATA+TGG	-	chr7_3:71482903-71482922	None:intergenic	45.0%
	GAGATGAGAAGAGGAGAGAT+GGG	-	chr7_3:71482991-71483010	None:intergenic	45.0%
	TGAGATGAGAAGAGGAGAGA+TGG	-	chr7_3:71482992-71483011	None:intergenic	45.0%
	TCTTTCGATGAAGCTCCTCC+CGG	+	chr7_3:71482430-71482449	Msa1121420:CDS	50.0%
	TTTGAAGATCTTCTCGCCGG+CGG	-	chr7_3:71482464-71482483	None:intergenic	50.0%
!	GAGAAGAGGAGAGATGGGTT+CGG	-	chr7_3:71482986-71483005	None:intergenic	50.0%
	CAGTGCCACACCGTCGACAA+AGG	+	chr7_3:71482496-71482515	Msa1121420:CDS	60.0%
!!	AAAGGCGCCGGCCACAAACA+AGG	+	chr7_3:71482514-71482533	Msa1121420:CDS	60.0%
	AAGATCTTCTCGCCGGCGGC+GGG	-	chr7_3:71482460-71482479	None:intergenic	65.0%
	GAAGATCTTCTCGCCGGCGG+CGG	-	chr7_3:71482461-71482480	None:intergenic	65.0%
!	GGTGTGGCACTGAGCGCACT+TGG	-	chr7_3:71482488-71482507	None:intergenic	65.0%
!	CACACCGTCGACAAAGGCGC+CGG	+	chr7_3:71482502-71482521	Msa1121420:CDS	65.0%
	AGATCTTCTCGCCGGCGGCG+GGG	-	chr7_3:71482459-71482478	None:intergenic	70.0%
	CGGCGCCTTTGTCGACGGTG+TGG	-	chr7_3:71482504-71482523	None:intergenic	70.0%
	GTGGCCGGCGCCTTTGTCGA+CGG	-	chr7_3:71482509-71482528	None:intergenic	70.0%
!	CCTCCCGGAAACCCCGCCGC+CGG	+	chr7_3:71482445-71482464	Msa1121420:CDS	80.0%
!	TCGCCGGCGGCGGGGTTTCC+GGG	-	chr7_3:71482451-71482470	None:intergenic	80.0%
!	CTCGCCGGCGGCGGGGTTTC+CGG	-	chr7_3:71482452-71482471	None:intergenic	80.0%
!	CCGGCGGCGGGGTTTCCGGG+AGG	-	chr7_3:71482448-71482467	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	71482424	71483022	71482424	ID=Msa1121420;Name=Msa1121420
chr7_3	mRNA	71482424	71483022	71482424	ID=Msa1121420-mRNA-1;Parent=Msa1121420;Name=Msa1121420-mRNA-1;_AED=0.72;_eAED=0.72;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|78
chr7_3	exon	71482424	71482535	71482424	ID=Msa1121420-mRNA-1:exon:18261;Parent=Msa1121420-mRNA-1
chr7_3	exon	71482898	71483022	71482898	ID=Msa1121420-mRNA-1:exon:18262;Parent=Msa1121420-mRNA-1
chr7_3	CDS	71482424	71482535	71482424	ID=Msa1121420-mRNA-1:cds;Parent=Msa1121420-mRNA-1
chr7_3	CDS	71482898	71483022	71482898	ID=Msa1121420-mRNA-1:cds;Parent=Msa1121420-mRNA-1

Gene Sequence	>Msa1121420 ATGGCGTCTTTCGATGAAGCTCCTCCCGGAAACCCCGCCGCCGGCGAGAAGATCTTCAAAACCAAGTGCGCTCAGTGCCACACCGTCGACAAAGGCGCCGGCCACAAACAAGAACCATATACACAGCCGCAAACACAACTTCTTCATCATACAAACAAAAAAAATCCATCTCTTCTCATCTCATCATCTCTTGATCGCCGAACCCATCTCTCCTCTTCTCATCTCATCATCTTTTGA
Protein sequence	>Msa1121420 MASFDEAPPGNPAAGEKIFKTKCAQCHTVDKGAGHKQEPYTQPQTQLLHHTNKKNPSLLISSSLDRRTHLSSSHLIIF*