AlfalfaGEDB | Msa1132210

M. sativa cultivar zhongmu-4 / Msa1132210

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1132210	RHN53323.1	64.644	379	66	8	1	312	1	378	6.28e-153	445
Msa1132210	RHN53323.1	70.988	162	27	5	12	155	221	380	1.52e-62	214

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1132210	sp\|P05332\|YP20_BACLI	31.373	153	82	3	21	154	8	156	2.64e-13	70.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1132210	A0A396HL91	64.644	379	66	8	1	312	1	378	3.00e-153	445
Msa1132210	A0A396HL91	70.988	162	27	5	12	155	221	380	7.25e-63	214

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0794350	Msa1132210	0.814282	1.667874e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1132210	MtrunA17_Chr5g0394631	64.644	379	66	8	1	312	1	378	5.77e-157	445
Msa1132210	MtrunA17_Chr5g0394631	70.988	162	27	5	12	155	221	380	1.40e-66	214
Msa1132210	MtrunA17_Chr5g0394621	73.964	169	30	1	1	155	1	169	1.36e-81	245
Msa1132210	MtrunA17_Chr5g0394621	65.385	182	48	5	155	323	3	182	4.15e-68	211
Msa1132210	MtrunA17_Chr1g0204971	60.355	169	53	2	1	155	1	169	1.82e-65	204
Msa1132210	MtrunA17_Chr1g0204971	56.627	166	61	5	156	312	4	167	2.11e-52	171
Msa1132210	MtrunA17_Chr1g0204951	61.404	171	52	3	1	157	1	171	4.83e-65	203
Msa1132210	MtrunA17_Chr1g0204951	57.229	166	60	5	156	312	4	167	7.25e-53	172
Msa1132210	MtrunA17_Chr7g0273661	42.282	149	75	1	17	154	4	152	8.23e-35	124
Msa1132210	MtrunA17_Chr7g0273661	40.940	149	82	3	169	312	4	151	8.85e-27	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1132210	AT2G32020.1	48.276	145	66	1	20	155	18	162	2.88e-42	145
Msa1132210	AT2G32020.1	49.383	162	70	7	172	324	18	176	3.38e-35	126
Msa1132210	AT2G32030.1	46.541	159	68	2	6	155	17	167	7.06e-42	144
Msa1132210	AT2G32030.1	49.068	161	70	7	173	324	24	181	9.88e-35	125
Msa1132210	AT3G22560.1	36.842	152	83	1	16	154	2	153	3.73e-26	102
Msa1132210	AT3G22560.1	34.437	151	93	3	168	312	2	152	8.97e-20	85.5

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Find 73 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTAGCCAAGTTCTGG+GGG	0.620200	7_3:+85061783	Msa1132210:CDS
AAAGCTGGTTTCCAAAAGGA+GGG	0.657724	7_3:+85059883	Msa1132210:CDS
AAAGGTAGCCAAGTTCTGGG+GGG	0.779565	7_3:+85061784	Msa1132210:CDS
AAGTGGCCAAATTCTGCTCA+TGG	0.506987	7_3:+85059551	Msa1132210:CDS
AATCTAAGCTCTATATATTG+GGG	0.593409	7_3:+85059736	Msa1132210:CDS
ACCCAATAGCATGATCATTA+TGG	0.341789	7_3:-85061886	None:intergenic
ACCTTGATGATGTAATGGTG+TGG	0.686805	7_3:+85059515	Msa1132210:CDS
ACCTTGATGATGTAATGGTG+TGG	0.697032	7_3:+85061747	Msa1132210:CDS
AGAATCAAATGTGCAGAAAT+TGG	0.218349	7_3:+85061947	Msa1132210:CDS
AGCTCTATATATTGGGGCAA+AGG	0.501996	7_3:+85059742	Msa1132210:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAATCAATGATCTCTTCTT+TGG	-	chr7_3:85059461-85059480	None:intergenic	30.0%
TCAAGGTCAGAAAGATTAAA+GGG	-	chr7_3:85059502-85059521	None:intergenic	30.0%
ATCAAGGTCAGAAAGATTAA+AGG	-	chr7_3:85059503-85059522	None:intergenic	30.0%
TCTCCAAGTATGAATATTCA+TGG	-	chr7_3:85059810-85059829	None:intergenic	30.0%
ATGAATATTCATACTTGGAG+AGG	+	chr7_3:85059809-85059828	Msa1132210:CDS	30.0%
TTGTCGATGTTGAAAATGTT+GGG	+	chr7_3:85059842-85059861	Msa1132210:CDS	30.0%
TGTTAAGAAAGGAAAGTAAC+AGG	+	chr7_3:85060094-85060113	Msa1132210:intron	30.0%
TATATACCTTTGATCTTCCT+AGG	-	chr7_3:85060329-85060348	None:intergenic	30.0%
GATAAATCTAAGTTGTGCTT+TGG	-	chr7_3:85060684-85060703	None:intergenic	30.0%
TTGGTTAATTGCGATGATTA+TGG	+	chr7_3:85061208-85061227	Msa1132210:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	85059424	85062215	85059424	ID=Msa1132210;Name=Msa1132210
chr7_3	mRNA	85059424	85062215	85059424	ID=Msa1132210-mRNA-1;Parent=Msa1132210;Name=Msa1132210-mRNA-1;_AED=0.07;_eAED=0.11;_QI=0\|0\|0\|1\|0\|0\|5\|0\|325
chr7_3	exon	85059424	85059664	85059424	ID=Msa1132210-mRNA-1:exon:25360;Parent=Msa1132210-mRNA-1
chr7_3	exon	85059701	85059924	85059701	ID=Msa1132210-mRNA-1:exon:25361;Parent=Msa1132210-mRNA-1
chr7_3	exon	85061665	85061906	85061665	ID=Msa1132210-mRNA-1:exon:25362;Parent=Msa1132210-mRNA-1
chr7_3	exon	85061928	85062155	85061928	ID=Msa1132210-mRNA-1:exon:25363;Parent=Msa1132210-mRNA-1
chr7_3	exon	85062173	85062215	85062173	ID=Msa1132210-mRNA-1:exon:25364;Parent=Msa1132210-mRNA-1
chr7_3	CDS	85059424	85059664	85059424	ID=Msa1132210-mRNA-1:cds;Parent=Msa1132210-mRNA-1
chr7_3	CDS	85059701	85059924	85059701	ID=Msa1132210-mRNA-1:cds;Parent=Msa1132210-mRNA-1
chr7_3	CDS	85061665	85061906	85061665	ID=Msa1132210-mRNA-1:cds;Parent=Msa1132210-mRNA-1
chr7_3	CDS	85061928	85062155	85061928	ID=Msa1132210-mRNA-1:cds;Parent=Msa1132210-mRNA-1
chr7_3	CDS	85062173	85062215	85062173	ID=Msa1132210-mRNA-1:cds;Parent=Msa1132210-mRNA-1

Gene Sequence

>Msa1132210

ATGGAAGAAACTTTTATTTCTTCCAAACCATGTGCCAAAGAAGAGATCATTGATTTGAACCAAATCACACTCCGACCCTTTAATCTTTCTGACCTTGATGATGTAATGGTGTGGCATACTGATGAAAAAGTGGCCAAATTCTGCTCATGGGAACCTTACACCAGCAAAGAAGATGGCATCAACTTCATTGAAAATATAGAAAGAAAGTTTCTATGTTGCAAAGCAATATGCATTGATGATCATAAAAGCAGGAAACAATCTGCAGAACTTGGCTATAATCTAAGCTCTATATATTGGGGCAAAGGGATTGTCACGTGTGCTGTGAAACAAATTATTAAGGTTGCATTCCATGAATATTCATACTTGGAGAGGCTTGAAGCACTTGTCGATGTTGAAAATGTTGGGTCACAAAGAGTGTTGGAGAAAGCTGGTTTCCAAAAGGAGGGAGTTCTTAGGAAGTATTTGACTTCTATTTTCTCCAAACCATGTGCCAATGAAGAGATCATTGATTTGAACCAAATCACACTCCGGCATCTTCATCTTTCTGACCTTGATGATGTAATGGTGTGGCAGCAGACTGATGAAAAGGTAGCCAAGTTCTGGGGGGAACCTTACACTAGCAAAGACGAAGGCATCAACTTCATTGAAAATATAGAAAGTGATTATCTACGTTGCGAAGCAATATGCCATAATGATCATGCTATTGGTTCACGTTATGATCTAAGCAGAATCAAATGTGCAGAAATTGGGTATGTTCTAGGCTCTAAATATTGGGGCAAAAGGATTACCACGTTTGCTGTGAAACAAATGGTTAAGGTTGCATTCAGTGAATTTCCATACTTGGAAAGGCTTGAAGCTCTTGTTGATGTGGATAATTTATGGTCGCAAAGAGTAGTTCAGAAAGTTGGTTTTCAAAAAGAGGGAGTTCTTAGGAAGAAAAAGCAGAGATATGATCATGTTCAGTCTTCTTTCTACTGA

Protein sequence

>Msa1132210

MEETFISSKPCAKEEIIDLNQITLRPFNLSDLDDVMVWHTDEKVAKFCSWEPYTSKEDGINFIENIERKFLCCKAICIDDHKSRKQSAELGYNLSSIYWGKGIVTCAVKQIIKVAFHEYSYLERLEALVDVENVGSQRVLEKAGFQKEGVLRKYLTSIFSKPCANEEIIDLNQITLRHLHLSDLDDVMVWQQTDEKVAKFWGEPYTSKDEGINFIENIESDYLRCEAICHNDHAIGSRYDLSRIKCAEIGYVLGSKYWGKRITTFAVKQMVKVAFSEFPYLERLEALVDVDNLWSQRVVQKVGFQKEGVLRKKKQRYDHVQSSFY*