AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1132400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 108 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGATCTCGCGGACTTTC+TGG 0.050188 7_3:+85242925 Msa1132400:CDS
CACCAAATGAACAAGTATTT+TGG 0.120917 7_3:+85243939 Msa1132400:three_prime_UTR
CACCGATTCCCTCAAATTTA+AGG 0.127970 7_3:-85244506 None:intergenic
GAGGAGGATGCTATTGTTTC+TGG 0.159738 7_3:+85242461 Msa1132400:five_prime_UTR
AGCTTCTTCCTTTCTTAAAA+TGG 0.215722 7_3:-85242986 None:intergenic
AACTGTACCATTCTTATCTT+TGG 0.228191 7_3:-85242678 None:intergenic
ATATGTTATTACCTTGTAAA+AGG 0.231724 7_3:-85243013 None:intergenic
GTCCATCCTAAGGTTGATTT+AGG 0.245617 7_3:+85244396 Msa1132400:three_prime_UTR
AGAAATGTGCATGCTGTTTC+TGG 0.260188 7_3:+85242641 Msa1132400:CDS
ATATTTGCAGGTATTGGATA+TGG 0.264348 7_3:-85244325 None:intergenic
GTTGTCCCCTTGAGACTTAC+AGG 0.265063 7_3:-85244638 None:intergenic
CCCCAAAATACTTGTTCATT+TGG 0.293873 7_3:-85243941 None:intergenic
GACTGGTTGGCATTCTGATT+TGG 0.295846 7_3:+85242952 Msa1132400:CDS
TGTATGGCCGGGTTTGATTA+AGG 0.306325 7_3:+85244037 Msa1132400:three_prime_UTR
TGGTCAGTTTCACTGAGATT+TGG 0.321894 7_3:+85243998 Msa1132400:three_prime_UTR
GGTCAGTTTCACTGAGATTT+GGG 0.329310 7_3:+85243999 Msa1132400:three_prime_UTR
TGTTAAAATGTGACTTCAAC+AGG 0.331543 7_3:-85244682 None:intergenic
GATACGGATGTCGGTGTATC+TGG 0.335249 7_3:+85242490 Msa1132400:five_prime_UTR
AACAAAATATTTGCAGGTAT+TGG 0.342676 7_3:-85244331 None:intergenic
GGTATATGTAAGAGTGATCT+TGG 0.354956 7_3:-85244557 None:intergenic
ATGTGGGTTTATTAGAGATA+TGG 0.376425 7_3:+85242861 Msa1132400:CDS
TTTGGGGTGCGCTTTCCTCT+TGG 0.377294 7_3:+85243957 Msa1132400:three_prime_UTR
TAAGGCCAGACAATGAAGTT+TGG 0.378549 7_3:-85244579 None:intergenic
AGCATGTTGCAGTTTAGTTA+TGG 0.385405 7_3:+85244419 Msa1132400:three_prime_UTR
ATTATGAACAAACATATATA+CGG 0.385571 7_3:+85244704 Msa1132400:three_prime_UTR
CATTCTTTCCTTAAATTTGA+GGG 0.393327 7_3:+85244498 Msa1132400:three_prime_UTR
ATTACAGTTACATATATGTT+TGG 0.394546 7_3:-85244740 None:intergenic
GGCATACACGAAGAGGTAAA+TGG 0.395236 7_3:+85243759 Msa1132400:intron
AGGGAATCGGTGGACGTTGT+AGG 0.396809 7_3:+85244517 Msa1132400:three_prime_UTR
TCCACGATATGCTGCCTTTG+CGG 0.414296 7_3:-85244602 None:intergenic
TTCCTTAAATTTGAGGGAAT+CGG 0.414663 7_3:+85244504 Msa1132400:three_prime_UTR
ACATTCTTTCCTTAAATTTG+AGG 0.422864 7_3:+85244497 Msa1132400:three_prime_UTR
ACATTTCTTATTTGTTTCCA+TGG 0.432342 7_3:-85242626 None:intergenic
TGAATCAACGAACAAAGAAA+TGG 0.432715 7_3:+85242418 Msa1132400:five_prime_UTR
GGACTGAATTCATAAACAAT+TGG 0.433082 7_3:+85243978 Msa1132400:three_prime_UTR
TATACCCAAACTTCATTGTC+TGG 0.439597 7_3:+85244574 Msa1132400:three_prime_UTR
GTTGCAGTTTAGTTATGGAT+TGG 0.445325 7_3:+85244424 Msa1132400:three_prime_UTR
AAGGCCAGACAATGAAGTTT+GGG 0.445961 7_3:-85244578 None:intergenic
GAAAATATTTGTCCATCCTA+AGG 0.462067 7_3:+85244386 Msa1132400:three_prime_UTR
TTAAGCTTGGGAGTAATTGT+AGG 0.465004 7_3:+85243843 Msa1132400:three_prime_UTR
CTTCAACAGGTTTATTAATG+TGG 0.466599 7_3:-85244669 None:intergenic
CATGCAATGAATAAGAGTTC+TGG 0.471863 7_3:+85242595 Msa1132400:five_prime_UTR
AACAAGGAGGGAAGGAAGCC+TGG 0.475545 7_3:-85242895 None:intergenic
ATGTGCAACAAAATATTTGC+AGG 0.475716 7_3:-85244337 None:intergenic
AAAATACTTGTTCATTTGGT+GGG 0.477833 7_3:-85243937 None:intergenic
CAAAATACTTGTTCATTTGG+TGG 0.488128 7_3:-85243938 None:intergenic
CATGCTCCTAAATCAACCTT+AGG 0.490993 7_3:-85244402 None:intergenic
TCAGTCAGAGAGTTAAGCTT+GGG 0.497711 7_3:+85243831 Msa1132400:three_prime_UTR
GCGGACTTTCTGGTTCAGAC+TGG 0.499426 7_3:+85242935 Msa1132400:CDS
ACTTTCTGGTTCAGACTGGT+TGG 0.500891 7_3:+85242939 Msa1132400:CDS
ACTAACCTCCTTTGAGGATG+AGG 0.505061 7_3:+85242286 Msa1132400:five_prime_UTR
AGATACACTAACCTCCTTTG+AGG 0.505127 7_3:+85242280 Msa1132400:five_prime_UTR
CCGCTACAGGTACATGGAGT+TGG 0.505496 7_3:-85244298 None:intergenic
GTGAAAGAGCTATGTATGGC+CGG 0.515760 7_3:+85244025 Msa1132400:three_prime_UTR
AAAGCTTATTACTTACAAGA+AGG 0.522298 7_3:+85243799 Msa1132400:three_prime_UTR
ATCAGTCAGAGAGTTAAGCT+TGG 0.524772 7_3:+85243830 Msa1132400:three_prime_UTR
TGTTTCCCAGTGGCACATCA+AGG 0.527986 7_3:-85242522 None:intergenic
GATATGCTGCCTTTGCGGTA+AGG 0.529418 7_3:-85244597 None:intergenic
CCATTGTGTTGCAGTGGCGG+AGG 0.531436 7_3:+85244271 Msa1132400:intron
TGAAAGAGCTATGTATGGCC+GGG 0.532786 7_3:+85244026 Msa1132400:three_prime_UTR
GGAAACAATATCACTGGTCT+TGG 0.535733 7_3:+85242538 Msa1132400:five_prime_UTR
TTGTTTCTGGAATGAAGATA+CGG 0.538438 7_3:+85242474 Msa1132400:five_prime_UTR
TGGGTTTATTAGAGATATGG+TGG 0.539872 7_3:+85242864 Msa1132400:CDS
ATAACCTCCACTTCATTACC+AGG 0.543268 7_3:-85242338 None:intergenic
TGACTTCCTGTAAGTCTCAA+GGG 0.546234 7_3:+85244632 Msa1132400:three_prime_UTR
GGACATCCGCTACAGGTACA+TGG 0.549289 7_3:-85244304 None:intergenic
CTTAAATTTGAGGGAATCGG+TGG 0.549426 7_3:+85244507 Msa1132400:three_prime_UTR
TGGATATGGACATCCGCTAC+AGG 0.549663 7_3:-85244311 None:intergenic
TTATAACCTTGATGTGCCAC+TGG 0.551762 7_3:+85242516 Msa1132400:five_prime_UTR
ATTGTCTGGCCTTACCGCAA+AGG 0.554708 7_3:+85244588 Msa1132400:three_prime_UTR
CTCCTAAATCAACCTTAGGA+TGG 0.555353 7_3:-85244398 None:intergenic
TTCTTCCTTTCTTAAAATGG+CGG 0.555665 7_3:-85242983 None:intergenic
GACATTGCCAAAGATAAGAA+TGG 0.555828 7_3:+85242671 Msa1132400:CDS
CCTCCTTTGAGGATGAGGAT+TGG 0.562407 7_3:+85242291 Msa1132400:five_prime_UTR
CTGCAATGCAGTAGAGGAGG+AGG 0.563700 7_3:+85242445 Msa1132400:five_prime_UTR
TCTAGAACCTGGTAATGAAG+TGG 0.563868 7_3:+85242331 Msa1132400:five_prime_UTR
CTGCCAATCCTCATCCTCAA+AGG 0.563906 7_3:-85242294 None:intergenic
GTTAGTGAAAGAGCTATGTA+TGG 0.564530 7_3:+85244021 Msa1132400:three_prime_UTR
TGCTCTACCTTAATCAAACC+CGG 0.566197 7_3:-85244044 None:intergenic
GATATGGTGGAGTCTTCACC+AGG 0.566535 7_3:+85242877 Msa1132400:CDS
AACTCTCTGACTGATACTGT+TGG 0.566894 7_3:-85243822 None:intergenic
GTGCATGCTGTTTCTGGTGG+CGG 0.569717 7_3:+85242647 Msa1132400:CDS
AGATCAGAACAGCACGAACA+AGG 0.569928 7_3:-85242911 None:intergenic
ATGACTTCCTGTAAGTCTCA+AGG 0.577109 7_3:+85244631 Msa1132400:three_prime_UTR
TGGTACAGTTTCTAGTGAAG+AGG 0.577443 7_3:+85242691 Msa1132400:CDS
CCACTGGGAAACAATATCAC+TGG 0.577649 7_3:+85242532 Msa1132400:five_prime_UTR
ACGTTGTAGGTTCAGCTGAA+AGG 0.579021 7_3:+85244530 Msa1132400:three_prime_UTR
ACAGCACGAACAAGGAGGGA+AGG 0.583263 7_3:-85242903 None:intergenic
GGAACACTGCAATGCAGTAG+AGG 0.587548 7_3:+85242439 Msa1132400:five_prime_UTR
GTTTATGAATTCAGTCCAAG+AGG 0.593219 7_3:-85243972 None:intergenic
CAGAACAGCACGAACAAGGA+GGG 0.600446 7_3:-85242907 None:intergenic
ATAAAATCAAATCTAGAACC+TGG 0.600452 7_3:+85242320 Msa1132400:five_prime_UTR
ACCGCAAAGGCAGCATATCG+TGG 0.614192 7_3:+85244601 Msa1132400:three_prime_UTR
AAATGTAGGCATACACGAAG+AGG 0.621033 7_3:+85243752 Msa1132400:intron
GAGTTCTGGTGATGATGCCA+TGG 0.625028 7_3:+85242609 Msa1132400:exon
TTCGTGCTGTTCTGATCTCG+CGG 0.630748 7_3:+85242916 Msa1132400:CDS
GACTTCCTGTAAGTCTCAAG+GGG 0.631701 7_3:+85244633 Msa1132400:three_prime_UTR
TGGAATGAAGATACGGATGT+CGG 0.633471 7_3:+85242481 Msa1132400:five_prime_UTR
AGAACCTGGTAATGAAGTGG+AGG 0.644587 7_3:+85242334 Msa1132400:five_prime_UTR
ACACTGCAATGCAGTAGAGG+AGG 0.645963 7_3:+85242442 Msa1132400:five_prime_UTR
ACGGATGTCGGTGTATCTGG+TGG 0.648645 7_3:+85242493 Msa1132400:five_prime_UTR
CCAACTCCATGTACCTGTAG+CGG 0.650362 7_3:+85244298 Msa1132400:three_prime_UTR
CCAATCCTCATCCTCAAAGG+AGG 0.652751 7_3:-85242291 None:intergenic
TATAACCTTGATGTGCCACT+GGG 0.676362 7_3:+85242517 Msa1132400:five_prime_UTR
TCAGAACAGCACGAACAAGG+AGG 0.678937 7_3:-85242908 None:intergenic
AATGTGCATGCTGTTTCTGG+TGG 0.683021 7_3:+85242644 Msa1132400:CDS
GTAAATAAGACAGCTCGATG+TGG 0.700794 7_3:+85243892 Msa1132400:three_prime_UTR
CCAGTGATATTGTTTCCCAG+TGG 0.716859 7_3:-85242532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAAAAAGTTTTTATTTA+AGG - chr7_3:85242749-85242768 None:intergenic 10.0%
!! TCTTATATGATTCAAAATAT+TGG - chr7_3:85243244-85243263 None:intergenic 15.0%
!! ATTATGAACAAACATATATA+CGG + chr7_3:85244704-85244723 Msa1132400:three_prime_UTR 15.0%
!!! GTTATAATTGTTTTTGAAGA+GGG + chr7_3:85242356-85242375 Msa1132400:five_prime_UTR 20.0%
!! ATATGTTATTACCTTGTAAA+AGG - chr7_3:85243016-85243035 None:intergenic 20.0%
!!! TTTTAATCTAAAAGATTGAG+AGG + chr7_3:85243106-85243125 Msa1132400:intron 20.0%
!! TTTAATCTAAAAGATTGAGA+GGG + chr7_3:85243107-85243126 Msa1132400:intron 20.0%
!!! ATTTTTTTGGTTGAGATAAA+TGG + chr7_3:85243161-85243180 Msa1132400:intron 20.0%
!! TATGATTCAAAATATTGGAA+GGG - chr7_3:85243239-85243258 None:intergenic 20.0%
!! ATATGATTCAAAATATTGGA+AGG - chr7_3:85243240-85243259 None:intergenic 20.0%
!!! ACAAATGACTATTTTGATAA+AGG + chr7_3:85243429-85243448 Msa1132400:intron 20.0%
!!! GTAAGTACTTTAGAATTAAA+CGG - chr7_3:85244094-85244113 None:intergenic 20.0%
!! ATTACAGTTACATATATGTT+TGG - chr7_3:85244743-85244762 None:intergenic 20.0%
!! ATAAAATCAAATCTAGAACC+TGG + chr7_3:85242320-85242339 Msa1132400:five_prime_UTR 25.0%
!!! GGTTATAATTGTTTTTGAAG+AGG + chr7_3:85242355-85242374 Msa1132400:five_prime_UTR 25.0%
!! ACATTTCTTATTTGTTTCCA+TGG - chr7_3:85242629-85242648 None:intergenic 25.0%
! GATTTATGCTTCAAAAATGT+GGG + chr7_3:85242845-85242864 Msa1132400:CDS 25.0%
!!! GGATTTGATAGGTATTTTTT+TGG + chr7_3:85243148-85243167 Msa1132400:intron 25.0%
!! TTTGAGAATTGAAATTTTGC+AGG + chr7_3:85243527-85243546 Msa1132400:intron 25.0%
!! TTGAGAATTGAAATTTTGCA+GGG + chr7_3:85243528-85243547 Msa1132400:intron 25.0%
!! ACTCATTTTACAGACATAAA+AGG - chr7_3:85243707-85243726 None:intergenic 25.0%
! AAAGCTTATTACTTACAAGA+AGG + chr7_3:85243799-85243818 Msa1132400:three_prime_UTR 25.0%
!!! CTGTCTTATTTACTCATTTT+AGG - chr7_3:85243885-85243904 None:intergenic 25.0%
! AAAATACTTGTTCATTTGGT+GGG - chr7_3:85243940-85243959 None:intergenic 25.0%
!! ACCAAATGAACAAGTATTTT+GGG + chr7_3:85243940-85243959 Msa1132400:three_prime_UTR 25.0%
! TTCACTCATTTATCAGTATT+TGG - chr7_3:85244070-85244089 None:intergenic 25.0%
! AACAAAATATTTGCAGGTAT+TGG - chr7_3:85244334-85244353 None:intergenic 25.0%
! ACATTCTTTCCTTAAATTTG+AGG + chr7_3:85244497-85244516 Msa1132400:three_prime_UTR 25.0%
! CATTCTTTCCTTAAATTTGA+GGG + chr7_3:85244498-85244517 Msa1132400:three_prime_UTR 25.0%
TTACACAAAAACAACCATTC+AGG + chr7_3:85242247-85242266 Msa1132400:five_prime_UTR 30.0%
TGAATCAACGAACAAAGAAA+TGG + chr7_3:85242418-85242437 Msa1132400:five_prime_UTR 30.0%
!! TTGTTTCTGGAATGAAGATA+CGG + chr7_3:85242474-85242493 Msa1132400:five_prime_UTR 30.0%
AACTGTACCATTCTTATCTT+TGG - chr7_3:85242681-85242700 None:intergenic 30.0%
GATTTAACAAATGAACCTGT+TGG + chr7_3:85242790-85242809 Msa1132400:intron 30.0%
AGAAATTCAATTTGTCCAAC+AGG - chr7_3:85242808-85242827 None:intergenic 30.0%
TAAATCCTGTTTGTAACAGT+TGG - chr7_3:85242831-85242850 None:intergenic 30.0%
GGATTTATGCTTCAAAAATG+TGG + chr7_3:85242844-85242863 Msa1132400:intron 30.0%
ATGTGGGTTTATTAGAGATA+TGG + chr7_3:85242861-85242880 Msa1132400:CDS 30.0%
TTCTTCCTTTCTTAAAATGG+CGG - chr7_3:85242986-85243005 None:intergenic 30.0%
AGCTTCTTCCTTTCTTAAAA+TGG - chr7_3:85242989-85243008 None:intergenic 30.0%
AGAATCCATAGTTATTGTTG+AGG + chr7_3:85243070-85243089 Msa1132400:intron 30.0%
ATAGTTATTGTTGAGGTTGA+GGG + chr7_3:85243077-85243096 Msa1132400:intron 30.0%
AACATCTTGAAGCAAAATGA+AGG - chr7_3:85243297-85243316 None:intergenic 30.0%
ACACACTCAAAAAAGATGTT+CGG - chr7_3:85243478-85243497 None:intergenic 30.0%
! TTGAAATTTTGCAGGGAATT+AGG + chr7_3:85243535-85243554 Msa1132400:intron 30.0%
CTGATACTATGATGAAATGT+AGG + chr7_3:85243738-85243757 Msa1132400:intron 30.0%
!!! TTTTAGGACTCATAACATGT+TGG - chr7_3:85243869-85243888 None:intergenic 30.0%
CAAAATACTTGTTCATTTGG+TGG - chr7_3:85243941-85243960 None:intergenic 30.0%
! CACCAAATGAACAAGTATTT+TGG + chr7_3:85243939-85243958 Msa1132400:three_prime_UTR 30.0%
! CCAAATGAACAAGTATTTTG+GGG + chr7_3:85243941-85243960 Msa1132400:three_prime_UTR 30.0%
GGACTGAATTCATAAACAAT+TGG + chr7_3:85243978-85243997 Msa1132400:three_prime_UTR 30.0%
!! ATATTTGCAGGTATTGGATA+TGG - chr7_3:85244328-85244347 None:intergenic 30.0%
ATGTGCAACAAAATATTTGC+AGG - chr7_3:85244340-85244359 None:intergenic 30.0%
! TAGGATGGACAAATATTTTC+AGG - chr7_3:85244386-85244405 None:intergenic 30.0%
GAAAATATTTGTCCATCCTA+AGG + chr7_3:85244386-85244405 Msa1132400:three_prime_UTR 30.0%
AAAGAATGTTCGACGAAAAA+TGG - chr7_3:85244486-85244505 None:intergenic 30.0%
TTCCTTAAATTTGAGGGAAT+CGG + chr7_3:85244504-85244523 Msa1132400:three_prime_UTR 30.0%
CTTCAACAGGTTTATTAATG+TGG - chr7_3:85244672-85244691 None:intergenic 30.0%
TGTTAAAATGTGACTTCAAC+AGG - chr7_3:85244685-85244704 None:intergenic 30.0%
! GGTTAGTGTATCTCTTTTGT+AGG - chr7_3:85242273-85242292 None:intergenic 35.0%
CATGCAATGAATAAGAGTTC+TGG + chr7_3:85242595-85242614 Msa1132400:five_prime_UTR 35.0%
GACATTGCCAAAGATAAGAA+TGG + chr7_3:85242671-85242690 Msa1132400:CDS 35.0%
TTTCATGTTGAGATACAAGC+AGG + chr7_3:85242768-85242787 Msa1132400:intron 35.0%
TTTCTCCAACTGTTACAAAC+AGG + chr7_3:85242823-85242842 Msa1132400:intron 35.0%
TGGGTTTATTAGAGATATGG+TGG + chr7_3:85242864-85242883 Msa1132400:CDS 35.0%
CTCAACCTCAACAATAACTA+TGG - chr7_3:85243078-85243097 None:intergenic 35.0%
CATAGTTATTGTTGAGGTTG+AGG + chr7_3:85243076-85243095 Msa1132400:intron 35.0%
TAGTTATTGTTGAGGTTGAG+GGG + chr7_3:85243078-85243097 Msa1132400:intron 35.0%
! GGAATTTAGTTCACAAGCTA+AGG + chr7_3:85243182-85243201 Msa1132400:intron 35.0%
AGCAAAATGAAGGAAAGAGT+GGG - chr7_3:85243287-85243306 None:intergenic 35.0%
AAGCAAAATGAAGGAAAGAG+TGG - chr7_3:85243288-85243307 None:intergenic 35.0%
GATCGATCAAATTACGATAG+AGG - chr7_3:85243685-85243704 None:intergenic 35.0%
TTAAGCTTGGGAGTAATTGT+AGG + chr7_3:85243843-85243862 Msa1132400:three_prime_UTR 35.0%
CCCCAAAATACTTGTTCATT+TGG - chr7_3:85243944-85243963 None:intergenic 35.0%
GTTTATGAATTCAGTCCAAG+AGG - chr7_3:85243975-85243994 None:intergenic 35.0%
GTTAGTGAAAGAGCTATGTA+TGG + chr7_3:85244021-85244040 Msa1132400:three_prime_UTR 35.0%
! ATTTTACCATTGTGTTGCAG+TGG + chr7_3:85244265-85244284 Msa1132400:intron 35.0%
!! ATCACATTGAGTCATTTTCG+AGG + chr7_3:85244358-85244377 Msa1132400:three_prime_UTR 35.0%
!! TCACATTGAGTCATTTTCGA+GGG + chr7_3:85244359-85244378 Msa1132400:three_prime_UTR 35.0%
AGCATGTTGCAGTTTAGTTA+TGG + chr7_3:85244419-85244438 Msa1132400:three_prime_UTR 35.0%
GTTGCAGTTTAGTTATGGAT+TGG + chr7_3:85244424-85244443 Msa1132400:three_prime_UTR 35.0%
GGTATATGTAAGAGTGATCT+TGG - chr7_3:85244560-85244579 None:intergenic 35.0%
TATACCCAAACTTCATTGTC+TGG + chr7_3:85244574-85244593 Msa1132400:three_prime_UTR 35.0%
! ATCTCTTTTGTAGGCCTGAA+TGG - chr7_3:85242264-85242283 None:intergenic 40.0%
AGATACACTAACCTCCTTTG+AGG + chr7_3:85242280-85242299 Msa1132400:five_prime_UTR 40.0%
! TCTAGAACCTGGTAATGAAG+TGG + chr7_3:85242331-85242350 Msa1132400:five_prime_UTR 40.0%
ATAACCTCCACTTCATTACC+AGG - chr7_3:85242341-85242360 None:intergenic 40.0%
TGGAATGAAGATACGGATGT+CGG + chr7_3:85242481-85242500 Msa1132400:five_prime_UTR 40.0%
TTATAACCTTGATGTGCCAC+TGG + chr7_3:85242516-85242535 Msa1132400:five_prime_UTR 40.0%
! TATAACCTTGATGTGCCACT+GGG + chr7_3:85242517-85242536 Msa1132400:five_prime_UTR 40.0%
! GGAAACAATATCACTGGTCT+TGG + chr7_3:85242538-85242557 Msa1132400:five_prime_UTR 40.0%
!! AGAAATGTGCATGCTGTTTC+TGG + chr7_3:85242641-85242660 Msa1132400:CDS 40.0%
!! TGGTACAGTTTCTAGTGAAG+AGG + chr7_3:85242691-85242710 Msa1132400:CDS 40.0%
!! ACTTGCCGCCATTTTAAGAA+AGG + chr7_3:85242978-85242997 Msa1132400:exon 40.0%
! AGAAGCTCGAACCTTTTACA+AGG + chr7_3:85243002-85243021 Msa1132400:three_prime_UTR 40.0%
CTAAAAGATTGAGAGGGAAG+AGG + chr7_3:85243113-85243132 Msa1132400:intron 40.0%
TTAGTTCACAAGCTAAGGAG+AGG + chr7_3:85243187-85243206 Msa1132400:intron 40.0%
TAGTTCACAAGCTAAGGAGA+GGG + chr7_3:85243188-85243207 Msa1132400:intron 40.0%
GCAAAATGAAGGAAAGAGTG+GGG - chr7_3:85243286-85243305 None:intergenic 40.0%
GCTTCAAGATGTTCACTTTG+AGG + chr7_3:85243304-85243323 Msa1132400:intron 40.0%
! TCACTTTGAGGACACTCTAT+AGG + chr7_3:85243316-85243335 Msa1132400:intron 40.0%
GCAGGGAATTAGGATATCAT+TGG + chr7_3:85243545-85243564 Msa1132400:intron 40.0%
AAATGTAGGCATACACGAAG+AGG + chr7_3:85243752-85243771 Msa1132400:intron 40.0%
AACTCTCTGACTGATACTGT+TGG - chr7_3:85243825-85243844 None:intergenic 40.0%
ATCAGTCAGAGAGTTAAGCT+TGG + chr7_3:85243830-85243849 Msa1132400:three_prime_UTR 40.0%
TCAGTCAGAGAGTTAAGCTT+GGG + chr7_3:85243831-85243850 Msa1132400:three_prime_UTR 40.0%
GTAAATAAGACAGCTCGATG+TGG + chr7_3:85243892-85243911 Msa1132400:three_prime_UTR 40.0%
TGGTCAGTTTCACTGAGATT+TGG + chr7_3:85243998-85244017 Msa1132400:three_prime_UTR 40.0%
GGTCAGTTTCACTGAGATTT+GGG + chr7_3:85243999-85244018 Msa1132400:three_prime_UTR 40.0%
TGCTCTACCTTAATCAAACC+CGG - chr7_3:85244047-85244066 None:intergenic 40.0%
! GTCCATCCTAAGGTTGATTT+AGG + chr7_3:85244396-85244415 Msa1132400:three_prime_UTR 40.0%
CTCCTAAATCAACCTTAGGA+TGG - chr7_3:85244401-85244420 None:intergenic 40.0%
CATGCTCCTAAATCAACCTT+AGG - chr7_3:85244405-85244424 None:intergenic 40.0%
!! CACCGATTCCCTCAAATTTA+AGG - chr7_3:85244509-85244528 None:intergenic 40.0%
CTTAAATTTGAGGGAATCGG+TGG + chr7_3:85244507-85244526 Msa1132400:three_prime_UTR 40.0%
AAGGCCAGACAATGAAGTTT+GGG - chr7_3:85244581-85244600 None:intergenic 40.0%
TAAGGCCAGACAATGAAGTT+TGG - chr7_3:85244582-85244601 None:intergenic 40.0%
ATGACTTCCTGTAAGTCTCA+AGG + chr7_3:85244631-85244650 Msa1132400:three_prime_UTR 40.0%
TGACTTCCTGTAAGTCTCAA+GGG + chr7_3:85244632-85244651 Msa1132400:three_prime_UTR 40.0%
! ACTAACCTCCTTTGAGGATG+AGG + chr7_3:85242286-85242305 Msa1132400:five_prime_UTR 45.0%
! AGAACCTGGTAATGAAGTGG+AGG + chr7_3:85242334-85242353 Msa1132400:five_prime_UTR 45.0%
GAGGAGGATGCTATTGTTTC+TGG + chr7_3:85242461-85242480 Msa1132400:five_prime_UTR 45.0%
CCAGTGATATTGTTTCCCAG+TGG - chr7_3:85242535-85242554 None:intergenic 45.0%
CCACTGGGAAACAATATCAC+TGG + chr7_3:85242532-85242551 Msa1132400:five_prime_UTR 45.0%
!! AATGTGCATGCTGTTTCTGG+TGG + chr7_3:85242644-85242663 Msa1132400:CDS 45.0%
AGATCAGAACAGCACGAACA+AGG - chr7_3:85242914-85242933 None:intergenic 45.0%
!! ACTTTCTGGTTCAGACTGGT+TGG + chr7_3:85242939-85242958 Msa1132400:CDS 45.0%
! GACTGGTTGGCATTCTGATT+TGG + chr7_3:85242952-85242971 Msa1132400:CDS 45.0%
AGATTGAGAGGGAAGAGGAA+AGG + chr7_3:85243118-85243137 Msa1132400:intron 45.0%
AAGGAGTGGAGGGATTTGAT+AGG + chr7_3:85243137-85243156 Msa1132400:intron 45.0%
ACAAGCTAAGGAGAGGGATT+TGG + chr7_3:85243194-85243213 Msa1132400:intron 45.0%
! AAGGAGAGGGATTTGGAATG+CGG + chr7_3:85243201-85243220 Msa1132400:intron 45.0%
AGGACACTCTATAGGCAAAC+AGG + chr7_3:85243324-85243343 Msa1132400:intron 45.0%
ATAGGCAAACAGGCAAACAC+TGG + chr7_3:85243334-85243353 Msa1132400:intron 45.0%
TAGGCAAACAGGCAAACACT+GGG + chr7_3:85243335-85243354 Msa1132400:intron 45.0%
! GAGTGTGTAGCTTGTGAAAC+TGG + chr7_3:85243490-85243509 Msa1132400:intron 45.0%
GGCATACACGAAGAGGTAAA+TGG + chr7_3:85243759-85243778 Msa1132400:intron 45.0%
GTGAAAGAGCTATGTATGGC+CGG + chr7_3:85244025-85244044 Msa1132400:three_prime_UTR 45.0%
TGAAAGAGCTATGTATGGCC+GGG + chr7_3:85244026-85244045 Msa1132400:three_prime_UTR 45.0%
TGTATGGCCGGGTTTGATTA+AGG + chr7_3:85244037-85244056 Msa1132400:three_prime_UTR 45.0%
TTACCATTGTGTTGCAGTGG+CGG + chr7_3:85244268-85244287 Msa1132400:intron 45.0%
ACGTTGTAGGTTCAGCTGAA+AGG + chr7_3:85244530-85244549 Msa1132400:three_prime_UTR 45.0%
GACTTCCTGTAAGTCTCAAG+GGG + chr7_3:85244633-85244652 Msa1132400:three_prime_UTR 45.0%
CCAATCCTCATCCTCAAAGG+AGG - chr7_3:85242294-85242313 None:intergenic 50.0%
! CCTCCTTTGAGGATGAGGAT+TGG + chr7_3:85242291-85242310 Msa1132400:five_prime_UTR 50.0%
CTGCCAATCCTCATCCTCAA+AGG - chr7_3:85242297-85242316 None:intergenic 50.0%
GGAACACTGCAATGCAGTAG+AGG + chr7_3:85242439-85242458 Msa1132400:five_prime_UTR 50.0%
ACACTGCAATGCAGTAGAGG+AGG + chr7_3:85242442-85242461 Msa1132400:five_prime_UTR 50.0%
GATACGGATGTCGGTGTATC+TGG + chr7_3:85242490-85242509 Msa1132400:five_prime_UTR 50.0%
TGTTTCCCAGTGGCACATCA+AGG - chr7_3:85242525-85242544 None:intergenic 50.0%
! GAGTTCTGGTGATGATGCCA+TGG + chr7_3:85242609-85242628 Msa1132400:exon 50.0%
! GATATGGTGGAGTCTTCACC+AGG + chr7_3:85242877-85242896 Msa1132400:CDS 50.0%
CAGAACAGCACGAACAAGGA+GGG - chr7_3:85242910-85242929 None:intergenic 50.0%
TCAGAACAGCACGAACAAGG+AGG - chr7_3:85242911-85242930 None:intergenic 50.0%
! TTCGTGCTGTTCTGATCTCG+CGG + chr7_3:85242916-85242935 Msa1132400:CDS 50.0%
TTCTGATCTCGCGGACTTTC+TGG + chr7_3:85242925-85242944 Msa1132400:CDS 50.0%
GAAAAACCAAAGCCCACCAG+CGG - chr7_3:85243366-85243385 None:intergenic 50.0%
CCAACTCCATGTACCTGTAG+CGG + chr7_3:85244298-85244317 Msa1132400:three_prime_UTR 50.0%
TGGATATGGACATCCGCTAC+AGG - chr7_3:85244314-85244333 None:intergenic 50.0%
!! ATTGTCTGGCCTTACCGCAA+AGG + chr7_3:85244588-85244607 Msa1132400:three_prime_UTR 50.0%
GATATGCTGCCTTTGCGGTA+AGG - chr7_3:85244600-85244619 None:intergenic 50.0%
TCCACGATATGCTGCCTTTG+CGG - chr7_3:85244605-85244624 None:intergenic 50.0%
GTTGTCCCCTTGAGACTTAC+AGG - chr7_3:85244641-85244660 None:intergenic 50.0%
CTGCAATGCAGTAGAGGAGG+AGG + chr7_3:85242445-85242464 Msa1132400:five_prime_UTR 55.0%
ACGGATGTCGGTGTATCTGG+TGG + chr7_3:85242493-85242512 Msa1132400:five_prime_UTR 55.0%
!! GTGCATGCTGTTTCTGGTGG+CGG + chr7_3:85242647-85242666 Msa1132400:CDS 55.0%
AACAAGGAGGGAAGGAAGCC+TGG - chr7_3:85242898-85242917 None:intergenic 55.0%
ACAGCACGAACAAGGAGGGA+AGG - chr7_3:85242906-85242925 None:intergenic 55.0%
!! GCGGACTTTCTGGTTCAGAC+TGG + chr7_3:85242935-85242954 Msa1132400:CDS 55.0%
GAGAGGGAAGAGGAAAGGAG+TGG + chr7_3:85243123-85243142 Msa1132400:intron 55.0%
AGGGAAGAGGAAAGGAGTGG+AGG + chr7_3:85243126-85243145 Msa1132400:intron 55.0%
GGGAAGAGGAAAGGAGTGGA+GGG + chr7_3:85243127-85243146 Msa1132400:intron 55.0%
! TTTGGGGTGCGCTTTCCTCT+TGG + chr7_3:85243957-85243976 Msa1132400:three_prime_UTR 55.0%
CCGCTACAGGTACATGGAGT+TGG - chr7_3:85244301-85244320 None:intergenic 55.0%
GGACATCCGCTACAGGTACA+TGG - chr7_3:85244307-85244326 None:intergenic 55.0%
AGGGAATCGGTGGACGTTGT+AGG + chr7_3:85244517-85244536 Msa1132400:three_prime_UTR 55.0%
ACCGCAAAGGCAGCATATCG+TGG + chr7_3:85244601-85244620 Msa1132400:three_prime_UTR 55.0%
CCTCCGCCACTGCAACACAA+TGG - chr7_3:85244274-85244293 None:intergenic 60.0%
CCATTGTGTTGCAGTGGCGG+AGG + chr7_3:85244271-85244290 Msa1132400:intron 60.0%
CAAACACTGGGCAGTGCCGC+TGG + chr7_3:85243347-85243366 Msa1132400:intron 65.0%
! ACACTGGGCAGTGCCGCTGG+TGG + chr7_3:85243350-85243369 Msa1132400:intron 70.0%
! CACTGGGCAGTGCCGCTGGT+GGG + chr7_3:85243351-85243370 Msa1132400:intron 70.0%
!! GCAGTGCCGCTGGTGGGCTT+TGG + chr7_3:85243357-85243376 Msa1132400:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 85242225 85244770 85242225 ID=Msa1132400;Name=Msa1132400
chr7_3 mRNA 85242225 85244770 85242225 ID=Msa1132400-mRNA-1;Parent=Msa1132400;Name=Msa1132400-mRNA-1;_AED=0.40;_eAED=0.40;_QI=395|1|1|1|0.33|0.25|4|814|82
chr7_3 exon 85242225 85242718 85242225 ID=Msa1132400-mRNA-1:exon:25439;Parent=Msa1132400-mRNA-1
chr7_3 exon 85242845 85243023 85242845 ID=Msa1132400-mRNA-1:exon:25440;Parent=Msa1132400-mRNA-1
chr7_3 exon 85243760 85244058 85243760 ID=Msa1132400-mRNA-1:exon:25441;Parent=Msa1132400-mRNA-1
chr7_3 exon 85244285 85244770 85244285 ID=Msa1132400-mRNA-1:exon:25442;Parent=Msa1132400-mRNA-1
chr7_3 five_prime_UTR 85242225 85242619 85242225 ID=Msa1132400-mRNA-1:five_prime_utr;Parent=Msa1132400-mRNA-1
chr7_3 CDS 85242620 85242718 85242620 ID=Msa1132400-mRNA-1:cds;Parent=Msa1132400-mRNA-1
chr7_3 CDS 85242845 85242994 85242845 ID=Msa1132400-mRNA-1:cds;Parent=Msa1132400-mRNA-1
chr7_3 three_prime_UTR 85242995 85243023 85242995 ID=Msa1132400-mRNA-1:three_prime_utr;Parent=Msa1132400-mRNA-1
chr7_3 three_prime_UTR 85243760 85244058 85243760 ID=Msa1132400-mRNA-1:three_prime_utr;Parent=Msa1132400-mRNA-1
chr7_3 three_prime_UTR 85244285 85244770 85244285 ID=Msa1132400-mRNA-1:three_prime_utr;Parent=Msa1132400-mRNA-1
Gene Sequence

>Msa1132400

ATGATGCCATGGAAACAAATAAGAAATGTGCATGCTGTTTCTGGTGGCGGTGACATTGCCAAAGATAAGAATGGTACAGTTTCTAGTGAAGAGGAAAATGATTTATGCTTCAAAAATGTGGGTTTATTAGAGATATGGTGGAGTCTTCACCAGGCTTCCTTCCCTCCTTGTTCGTGCTGTTCTGATCTCGCGGACTTTCTGGTTCAGACTGGTTGGCATTCTGATTTGGATCACTTGCCGCCATTTTAA
Protein sequence

>Msa1132400

MMPWKQIRNVHAVSGGGDIAKDKNGTVSSEEENDLCFKNVGLLEIWWSLHQASFPPCSCCSDLADFLVQTGWHSDLDHLPPF*