AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1138620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1138620 MtrunA17_Chr6g0469071 63.380 142 46 1 1 136 113 254 5.23e-56 184
Msa1138620 MtrunA17_Chr1g0153901 62.937 143 46 2 1 136 113 255 1.41e-55 182
Msa1138620 MtrunA17_Chr2g0325841 45.255 137 72 2 4 138 600 735 2.60e-32 120
Msa1138620 MtrunA17_Chr4g0010671 46.528 144 75 2 1 142 111 254 5.50e-30 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 80 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAAAATTGTTTCTATTT+TGG 0.042404 7_4:-8271839 Msa1138620:five_prime_UTR
ATGGAATTTCTAAATGTTTA+TGG 0.098792 7_4:+8272545 None:intergenic
TCGAAAGACCCCTGAATTTA+AGG 0.126971 7_4:-8269775 Msa1138620:intron
GGATCTGGATCATCACTTTC+TGG 0.146392 7_4:+8269969 None:intergenic
TTCTAAATGTTTATGGGTTT+TGG 0.151731 7_4:+8272552 None:intergenic
TTTCTGAAGATTTGGGTTTC+TGG 0.158057 7_4:+8272576 None:intergenic
CTTCCTTCTTCAAAACAAAA+AGG 0.224595 7_4:-8272411 Msa1138620:intron
ATGTAATTTGGATAAGTAAT+TGG 0.254965 7_4:-8269352 Msa1138620:three_prime_UTR
GTGTGTTTGGATATGTAATT+TGG 0.260929 7_4:-8269364 Msa1138620:three_prime_UTR
TTGAGGTTGGATTTCCGATT+TGG 0.283215 7_4:+8272506 None:intergenic
TGAGGTTGGATTTCCGATTT+GGG 0.291966 7_4:+8272507 None:intergenic
TGGAATTTCTAAATGTTTAT+GGG 0.304837 7_4:+8272546 None:intergenic
TGCATGGATTCCTGAATTTA+TGG 0.314614 7_4:+8271939 None:intergenic
ATCACTTCCTATTTGTGCTT+TGG 0.318322 7_4:-8270651 Msa1138620:five_prime_UTR
TGAGAGGGGTAGAGGGTTTG+TGG 0.336486 7_4:+8272683 None:intergenic
ACACAAAACTTTGTTATCAA+TGG 0.339705 7_4:+8270621 None:intergenic
CGTATACTAACCTTAAATTC+AGG 0.342289 7_4:+8269765 None:intergenic
CTTTGTGGTGAGAGGGGTAG+AGG 0.345291 7_4:+8272675 None:intergenic
TCTGGAATGATTTGCGGATC+TGG 0.365385 7_4:+8272594 None:intergenic
AAGTGGATGACGGCAGTCTT+TGG 0.369068 7_4:+8269848 None:intergenic
TGTAGTTTGACCATAAATTC+AGG 0.371579 7_4:-8271949 Msa1138620:five_prime_UTR
GAGATTTCTAGCATTTGTTC+AGG 0.377979 7_4:-8271894 Msa1138620:five_prime_UTR
CGCCGTCACGTCATGACAAA+TGG 0.380342 7_4:-8269515 Msa1138620:CDS
TGGTGAGAGTGAACTGGATC+TGG 0.395737 7_4:+8272648 None:intergenic
AACGAAACCAAAGCACAAAT+AGG 0.430455 7_4:+8270644 None:intergenic
ATAAATAGATGATGTGTGTT+TGG 0.432806 7_4:-8269377 Msa1138620:three_prime_UTR
GTGAGAGTGAACGGCGGATC+TGG 0.451371 7_4:+8272628 None:intergenic
AGATATATGGGAGAAGTTTG+TGG 0.470943 7_4:-8269802 Msa1138620:CDS
ATGAACCTTCTTGTTTGTTG+TGG 0.474107 7_4:-8269200 Msa1138620:three_prime_UTR
GGATGATGGTTTGTGGGGAC+CGG 0.476464 7_4:-8269920 Msa1138620:CDS
CGGATCTGGTGAGAGTGAAC+TGG 0.479191 7_4:+8272642 None:intergenic
CTGGAGAGCTTTGTGGTGAG+AGG 0.482427 7_4:+8272667 None:intergenic
TCTCAAGGTCTGACTACTAC+TGG 0.483198 7_4:+8271913 None:intergenic
TGCGGATCTGGAGAGCTTTG+TGG 0.486766 7_4:+8272606 None:intergenic
ACTGGATCTGGAGAGCTTTG+TGG 0.493467 7_4:+8272660 None:intergenic
CCTCCACGAGATAATGTATG+TGG 0.495099 7_4:+8269621 None:intergenic
TTTGTGGTGAGAGGGGTAGA+GGG 0.497534 7_4:+8272676 None:intergenic
CACGAACGAGAGGAGATTTG+AGG 0.499345 7_4:+8272489 None:intergenic
TGGGTTTCTGGAATGATTTG+CGG 0.506631 7_4:+8272588 None:intergenic
AGAGAGCACGACAAAAGAAA+GGG 0.507249 7_4:-8269491 Msa1138620:CDS
GATGAGATTCAACCCCAAAT+CGG 0.508707 7_4:-8272520 Msa1138620:five_prime_UTR
TAGAATATGATCAAATTCTC+CGG 0.517940 7_4:+8269542 None:intergenic
TGGGGTTGAATCTCATCTCA+TGG 0.519668 7_4:+8272526 None:intergenic
GGATGACGGCAGTCTTTGGA+AGG 0.520877 7_4:+8269852 None:intergenic
AAGAGAGCACGACAAAAGAA+AGG 0.521144 7_4:-8269492 Msa1138620:CDS
GTATACTAACCTTAAATTCA+GGG 0.523147 7_4:+8269766 None:intergenic
AGCTTTGTGGTGAGAGTGAA+CGG 0.525393 7_4:+8272619 None:intergenic
GAGATAATGTATGTGGGTAA+AGG 0.527971 7_4:+8269628 None:intergenic
CAATGATTTGCGGAGAAACA+CGG 0.529179 7_4:+8272443 None:intergenic
TGTATGTGGGTAAAGGTTGT+TGG 0.530757 7_4:+8269635 None:intergenic
TGACTACTACTGGTGGTGCA+TGG 0.530917 7_4:+8271923 None:intergenic
TGATGGTTTGTGGGGACCGG+TGG 0.532136 7_4:-8269917 Msa1138620:CDS
AACGAGAGGAGATTTGAGGT+TGG 0.542755 7_4:+8272493 None:intergenic
GTTTGTGGGGACCGGTGGAG+AGG 0.557784 7_4:-8269912 Msa1138620:CDS
TGGAGAGCTTTGTGGTGAGA+GGG 0.558180 7_4:+8272668 None:intergenic
AACAAATGCTAGAAATCTCA+AGG 0.561817 7_4:+8271898 None:intergenic
TTACCTTGAAGATAAAATGA+TGG 0.575253 7_4:-8269592 Msa1138620:CDS
AGAAAAGGATACTTTACAAG+TGG 0.577527 7_4:+8269831 None:intergenic
TTTGGGGATCAGATGCCAAA+GGG 0.580047 7_4:-8271821 Msa1138620:intron
GAGTCACGAACAATGATTTG+CGG 0.583640 7_4:+8272433 None:intergenic
TTCCATTTGTCATGACGTGA+CGG 0.588309 7_4:+8269513 None:intergenic
GTGTCTTAAAACCTCTCCAC+CGG 0.595488 7_4:+8269901 None:intergenic
TGCTTCATTGAGGCAAAGTG+AGG 0.597340 7_4:+8271857 None:intergenic
TACATCCACAACAAACAAGA+AGG 0.605162 7_4:+8269195 None:intergenic
TACCCACATACATTATCTCG+TGG 0.605189 7_4:-8269624 Msa1138620:CDS
GAGAGCACGACAAAAGAAAG+GGG 0.611004 7_4:-8269490 Msa1138620:CDS
ACGTGGTACTGAAGATATGT+TGG 0.612925 7_4:-8269312 Msa1138620:three_prime_UTR
TATACTAACCTTAAATTCAG+GGG 0.621254 7_4:+8269767 None:intergenic
GATGACGGCAGTCTTTGGAA+GGG 0.627217 7_4:+8269853 None:intergenic
GGAGAGCTTTGTGGTGAGAG+GGG 0.630932 7_4:+8272669 None:intergenic
GAGGTTGGATTTCCGATTTG+GGG 0.646354 7_4:+8272508 None:intergenic
CTCCACGAGATAATGTATGT+GGG 0.647609 7_4:+8269622 None:intergenic
GATACTTTACAAGTGGATGA+CGG 0.655215 7_4:+8269838 None:intergenic
AGAGCACGACAAAAGAAAGG+GGG 0.662182 7_4:-8269489 Msa1138620:CDS
GAGTAACAATCACGAACGAG+AGG 0.668417 7_4:+8272479 None:intergenic
CATTTGTCATGACGTGACGG+CGG 0.670962 7_4:+8269516 None:intergenic
CAAGGTCTGACTACTACTGG+TGG 0.680676 7_4:+8271916 None:intergenic
CCACATACATTATCTCGTGG+AGG 0.684741 7_4:-8269621 Msa1138620:CDS
AAAATGGATGATGGTTTGTG+GGG 0.689791 7_4:-8269925 Msa1138620:exon
TTTGTGGTGAGAGTGAACGG+CGG 0.805316 7_4:+8272622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATGTTTTTTGAAA+GGG - chr7_4:8269498-8269517 Msa1138620:CDS 10.0%
!!! TAATGAATTTGATTTATAAT+AGG - chr7_4:8272084-8272103 Msa1138620:intron 10.0%
!!! TAAATTTCTCTTTTTTCAAA+TGG - chr7_4:8269457-8269476 Msa1138620:CDS 15.0%
!!! AAATTTCTCTTTTTTCAAAT+GGG - chr7_4:8269458-8269477 Msa1138620:CDS 15.0%
!!! GATTTTTTATGTTTTTTGAA+AGG - chr7_4:8269497-8269516 Msa1138620:CDS 15.0%
!!! TTTTTTATGTTTTTTGAAAG+GGG - chr7_4:8269499-8269518 Msa1138620:CDS 15.0%
!! ATATTAGTAATATTCTTCAT+GGG + chr7_4:8269653-8269672 None:intergenic 15.0%
!! AATATTAGTAATATTCTTCA+TGG + chr7_4:8269654-8269673 None:intergenic 15.0%
!!! TAATCTTCTATTTGTAATTT+TGG - chr7_4:8269974-8269993 Msa1138620:five_prime_UTR 15.0%
!!! AATCTTCTATTTGTAATTTT+GGG - chr7_4:8269975-8269994 Msa1138620:five_prime_UTR 15.0%
!! GAACAAATCAAATTTAATAT+TGG + chr7_4:8270803-8270822 None:intergenic 15.0%
!! AATAAATAATAACACAAACA+TGG + chr7_4:8271303-8271322 None:intergenic 15.0%
!! TGTCTTAATAGATTAAAAAT+TGG - chr7_4:8271483-8271502 Msa1138620:intron 15.0%
!!! TTTTCTTCAAAAAGATATAT+GGG - chr7_4:8271958-8271977 Msa1138620:intron 15.0%
!! TAAACAATTAAATTAGTACA+GGG + chr7_4:8272050-8272069 None:intergenic 15.0%
!! ATAAACAATTAAATTAGTAC+AGG + chr7_4:8272051-8272070 None:intergenic 15.0%
!! TGGAATTTCTAAATGTTTAT+GGG + chr7_4:8269229-8269248 None:intergenic 20.0%
!! ATGGAATTTCTAAATGTTTA+TGG + chr7_4:8269230-8269249 None:intergenic 20.0%
!!! TTTTTCTTCTTCTTTCAAAT+TGG - chr7_4:8269532-8269551 Msa1138620:CDS 20.0%
!!! TTTTCTTCTTCTTTCAAATT+GGG - chr7_4:8269533-8269552 Msa1138620:CDS 20.0%
!!! AAGCAAAATTGTTTCTATTT+TGG - chr7_4:8269933-8269952 Msa1138620:exon 20.0%
!!! AGCAAAATTGTTTCTATTTT+GGG - chr7_4:8269934-8269953 Msa1138620:exon 20.0%
!!! ATTAGTCTTATTATATATGC+AGG + chr7_4:8270077-8270096 None:intergenic 20.0%
!! CAATATCAATATTCTTTCAA+TGG + chr7_4:8270446-8270465 None:intergenic 20.0%
!! GATTGTTATATTAGTATTAG+TGG - chr7_4:8270748-8270767 Msa1138620:intron 20.0%
!!! TTTGATTTGTTCTTTTATGT+TGG - chr7_4:8270811-8270830 Msa1138620:intron 20.0%
!!! TTGGTAGATAAGTTTTTTAA+TGG - chr7_4:8270850-8270869 Msa1138620:intron 20.0%
!!! TGGTAGATAAGTTTTTTAAT+GGG - chr7_4:8270851-8270870 Msa1138620:intron 20.0%
!!! ATAACAAAGTTTTGTGTTTA+AGG - chr7_4:8271157-8271176 Msa1138620:intron 20.0%
!! TAATTAAAGTCATTTGCTTA+TGG - chr7_4:8271258-8271277 Msa1138620:intron 20.0%
!!! TTTTTAATCTATTAAGACAC+CGG + chr7_4:8271482-8271501 None:intergenic 20.0%
!!! TTTTTCAAAGCATCTTTTTT+TGG + chr7_4:8271827-8271846 None:intergenic 20.0%
!!! AAGATGCTTTGAAAAAAAAA+TGG - chr7_4:8271831-8271850 Msa1138620:five_prime_UTR 20.0%
!! TTTGAAAAAAAAATGGATGA+TGG - chr7_4:8271838-8271857 Msa1138620:five_prime_UTR 20.0%
!!! CATATATCTTTTTGAAGAAA+AGG + chr7_4:8271959-8271978 None:intergenic 20.0%
!!! CTTTTCTTCAAAAAGATATA+TGG - chr7_4:8271957-8271976 Msa1138620:intron 20.0%
!! ATAATAGGAAAAAAGTCAAA+AGG - chr7_4:8272099-8272118 Msa1138620:intron 20.0%
!! TAATAGGAAAAAAGTCAAAA+GGG - chr7_4:8272100-8272119 Msa1138620:intron 20.0%
!! ATGTAATTTGGATAAGTAAT+TGG - chr7_4:8272420-8272439 Msa1138620:five_prime_UTR 20.0%
!!! TTTTGTTTGATTTAGAAGAA+TGG - chr7_4:8272643-8272662 Msa1138620:five_prime_UTR 20.0%
!!! TTTTGGATTTCTGAAGATTT+GGG + chr7_4:8269206-8269225 None:intergenic 25.0%
!! TTCTAAATGTTTATGGGTTT+TGG + chr7_4:8269223-8269242 None:intergenic 25.0%
!!! TTTTTTGCTCTTTTCTCAAA+AGG - chr7_4:8269425-8269444 Msa1138620:CDS 25.0%
!! TTTTTGCTCTTTTCTCAAAA+GGG - chr7_4:8269426-8269445 Msa1138620:CDS 25.0%
! AAGTATACATATGATACACT+TGG - chr7_4:8269688-8269707 Msa1138620:intron 25.0%
!!! GCAAAATTGTTTCTATTTTG+GGG - chr7_4:8269935-8269954 Msa1138620:exon 25.0%
! TTGTATCAAAATAGAATCCA+AGG + chr7_4:8270488-8270507 None:intergenic 25.0%
! GATTTAGTAGAAAATTGTTG+AGG + chr7_4:8270562-8270581 None:intergenic 25.0%
! ATGCCTATCAATTGTTATAT+TGG - chr7_4:8270679-8270698 Msa1138620:intron 25.0%
! TGCCTATCAATTGTTATATT+GGG - chr7_4:8270680-8270699 Msa1138620:intron 25.0%
! ATCCCAATATAACAATTGAT+AGG + chr7_4:8270685-8270704 None:intergenic 25.0%
!!! GGTAGATAAGTTTTTTAATG+GGG - chr7_4:8270852-8270871 Msa1138620:intron 25.0%
!!! AAGTTTTTTAATGGGGTTTT+TGG - chr7_4:8270859-8270878 Msa1138620:intron 25.0%
! TCCTATTTATTGTTTGTTCT+TGG - chr7_4:8270947-8270966 Msa1138620:intron 25.0%
! CCTATTTATTGTTTGTTCTT+GGG - chr7_4:8270948-8270967 Msa1138620:intron 25.0%
!! ACACAAAACTTTGTTATCAA+TGG + chr7_4:8271154-8271173 None:intergenic 25.0%
!!! CAATGTTTTTAACTATAGCA+GGG + chr7_4:8271369-8271388 None:intergenic 25.0%
!!! TCAATGTTTTTAACTATAGC+AGG + chr7_4:8271370-8271389 None:intergenic 25.0%
! TTTAACTAACAACATGAAGT+TGG - chr7_4:8271399-8271418 Msa1138620:intron 25.0%
! TATACTAACCTTAAATTCAG+GGG + chr7_4:8272008-8272027 None:intergenic 25.0%
! GTATACTAACCTTAAATTCA+GGG + chr7_4:8272009-8272028 None:intergenic 25.0%
! TTACCTTGAAGATAAAATGA+TGG - chr7_4:8272180-8272199 Msa1138620:intron 25.0%
! TAGAATATGATCAAATTCTC+CGG + chr7_4:8272233-8272252 None:intergenic 25.0%
! ATAAATAGATGATGTGTGTT+TGG - chr7_4:8272395-8272414 Msa1138620:intron 25.0%
!! GTTTTGGATTTCTGAAGATT+TGG + chr7_4:8269207-8269226 None:intergenic 30.0%
CTTCCTTCTTCAAAACAAAA+AGG - chr7_4:8269361-8269380 Msa1138620:three_prime_UTR 30.0%
!!! GAACCTTTTTGTTTTGAAGA+AGG + chr7_4:8269367-8269386 None:intergenic 30.0%
!!! TCTCTTTTTTCAAATGGGTT+TGG - chr7_4:8269463-8269482 Msa1138620:CDS 30.0%
!!! CTCTTTTTTCAAATGGGTTT+GGG - chr7_4:8269464-8269483 Msa1138620:CDS 30.0%
TGTAGTTTGACCATAAATTC+AGG - chr7_4:8269823-8269842 Msa1138620:CDS 30.0%
AACAAATGCTAGAAATCTCA+AGG + chr7_4:8269877-8269896 None:intergenic 30.0%
! GAAACAATTTTGCTTCATTG+AGG + chr7_4:8269928-8269947 None:intergenic 30.0%
ACAAATAGAAGATTACCCTT+TGG + chr7_4:8269969-8269988 None:intergenic 30.0%
CAATGAGTCTCTATATGAAA+AGG + chr7_4:8270005-8270024 None:intergenic 30.0%
ACATAACACAAGCATATCTT+GGG + chr7_4:8270322-8270341 None:intergenic 30.0%
TACATAACACAAGCATATCT+TGG + chr7_4:8270323-8270342 None:intergenic 30.0%
TTTCAATGGAGAACCTATTT+GGG + chr7_4:8270432-8270451 None:intergenic 30.0%
GATACAAATGAATATGTCCT+CGG - chr7_4:8270501-8270520 Msa1138620:intron 30.0%
ATACAAATGAATATGTCCTC+GGG - chr7_4:8270502-8270521 Msa1138620:intron 30.0%
TTAGTAGAAAATTGTTGAGG+TGG + chr7_4:8270559-8270578 None:intergenic 30.0%
TGTATTTCATTGTTAGTGGA+AGG - chr7_4:8270917-8270936 Msa1138620:intron 30.0%
CCCAAGAACAAACAATAAAT+AGG + chr7_4:8270951-8270970 None:intergenic 30.0%
AAAAAAAAATGACCTGTCCA+GGG + chr7_4:8270989-8271008 None:intergenic 30.0%
AAAAAAAAAATGACCTGTCC+AGG + chr7_4:8270990-8271009 None:intergenic 30.0%
AACAAAAAGCGATCATTAAG+AGG + chr7_4:8271070-8271089 None:intergenic 30.0%
! GTGCTTTTATGTTCTATGAA+TGG - chr7_4:8271505-8271524 Msa1138620:intron 30.0%
!!! AAAGCATCTTTTTTTGGATC+TGG + chr7_4:8271821-8271840 None:intergenic 30.0%
! AAAAAATGGATGATGGTTTG+TGG - chr7_4:8271845-8271864 Msa1138620:five_prime_UTR 30.0%
! AAAAATGGATGATGGTTTGT+GGG - chr7_4:8271846-8271865 Msa1138620:five_prime_UTR 30.0%
AGAAAAGGATACTTTACAAG+TGG + chr7_4:8271944-8271963 None:intergenic 30.0%
CGTATACTAACCTTAAATTC+AGG + chr7_4:8272010-8272029 None:intergenic 30.0%
! GAATTTAAGGTTAGTATACG+TGG - chr7_4:8272010-8272029 Msa1138620:intron 30.0%
! CATCCATCATTTTATCTTCA+AGG + chr7_4:8272186-8272205 None:intergenic 30.0%
GGATGAAAAGAGAAAAAGAA+TGG - chr7_4:8272201-8272220 Msa1138620:intron 30.0%
GTGTGTTTGGATATGTAATT+TGG - chr7_4:8272408-8272427 Msa1138620:intron 30.0%
!!! GAGAGACCTTTTTTTTGTTT+TGG - chr7_4:8272493-8272512 Msa1138620:five_prime_UTR 30.0%
GCAGTACCAAAACAAAAAAA+AGG + chr7_4:8272502-8272521 None:intergenic 30.0%
!!! TTTTTTTGTTTTGGTACTGC+TGG - chr7_4:8272502-8272521 Msa1138620:five_prime_UTR 30.0%
!!! TTTTTTGTTTTGGTACTGCT+GGG - chr7_4:8272503-8272522 Msa1138620:five_prime_UTR 30.0%
! TGTTGACATAGACATTTTGA+AGG - chr7_4:8272620-8272639 Msa1138620:five_prime_UTR 30.0%
! TTTCTGAAGATTTGGGTTTC+TGG + chr7_4:8269199-8269218 None:intergenic 35.0%
!! TGACTCTCAAATGGGTTTTT+AGG - chr7_4:8269388-8269407 Msa1138620:three_prime_UTR 35.0%
!! GACTCTCAAATGGGTTTTTA+GGG - chr7_4:8269389-8269408 Msa1138620:three_prime_UTR 35.0%
!!! TCAAATGGGTTTTTAGGGTT+AGG - chr7_4:8269394-8269413 Msa1138620:three_prime_UTR 35.0%
! CTCTTTTCTCAAAAGGGTTT+GGG - chr7_4:8269432-8269451 Msa1138620:CDS 35.0%
! TCAAATGGGTTTGGGTTTAA+AGG - chr7_4:8269472-8269491 Msa1138620:CDS 35.0%
CTAATGAAACTAGCTCAGTT+TGG - chr7_4:8269725-8269744 Msa1138620:intron 35.0%
TGCATGGATTCCTGAATTTA+TGG + chr7_4:8269836-8269855 None:intergenic 35.0%
!! GAGATTTCTAGCATTTGTTC+AGG - chr7_4:8269878-8269897 Msa1138620:CDS 35.0%
GGAAAACCTAGAATTCTTGT+GGG + chr7_4:8270392-8270411 None:intergenic 35.0%
TGGAAAACCTAGAATTCTTG+TGG + chr7_4:8270393-8270412 None:intergenic 35.0%
CTTTCAATGGAGAACCTATT+TGG + chr7_4:8270433-8270452 None:intergenic 35.0%
TACAAATGAATATGTCCTCG+GGG - chr7_4:8270503-8270522 Msa1138620:intron 35.0%
CAGGACAACATGTGAAATAT+AGG + chr7_4:8270589-8270608 None:intergenic 35.0%
ATCTACCAAGACAATCTCTT+GGG + chr7_4:8270839-8270858 None:intergenic 35.0%
TATCTACCAAGACAATCTCT+TGG + chr7_4:8270840-8270859 None:intergenic 35.0%
CCACTAACAATGAAATACAG+AGG + chr7_4:8270916-8270935 None:intergenic 35.0%
CCTCTGTATTTCATTGTTAG+TGG - chr7_4:8270913-8270932 Msa1138620:intron 35.0%
!!! CGCTTTTTGTTTTCATTTGC+AGG - chr7_4:8271079-8271098 Msa1138620:intron 35.0%
ATCACTTCCTATTTGTGCTT+TGG - chr7_4:8271121-8271140 Msa1138620:intron 35.0%
AACGAAACCAAAGCACAAAT+AGG + chr7_4:8271131-8271150 None:intergenic 35.0%
!! TTTTCTCTCTCGCTTTTGTT+TGG - chr7_4:8271188-8271207 Msa1138620:intron 35.0%
!! TTTCTCTCTCGCTTTTGTTT+GGG - chr7_4:8271189-8271208 Msa1138620:intron 35.0%
AACTAACAACATGAAGTTGG+TGG - chr7_4:8271402-8271421 Msa1138620:intron 35.0%
TTATGTTCTATGAATGGTGC+TGG - chr7_4:8271511-8271530 Msa1138620:intron 35.0%
ATCCTAGAGATTGCTTATCT+AGG - chr7_4:8271535-8271554 Msa1138620:intron 35.0%
TACCTAGATAAGCAATCTCT+AGG + chr7_4:8271540-8271559 None:intergenic 35.0%
TAGAGATTGCTTATCTAGGT+AGG - chr7_4:8271539-8271558 Msa1138620:intron 35.0%
CAACAAACTAAACCAACTGA+TGG + chr7_4:8271642-8271661 None:intergenic 35.0%
GCAGCAGAAAAAATTACACT+AGG + chr7_4:8271713-8271732 None:intergenic 35.0%
!!! AGTGTAATTTTTTCTGCTGC+TGG - chr7_4:8271713-8271732 Msa1138620:intron 35.0%
! AAAATGGATGATGGTTTGTG+GGG - chr7_4:8271847-8271866 Msa1138620:five_prime_UTR 35.0%
GATACTTTACAAGTGGATGA+CGG + chr7_4:8271937-8271956 None:intergenic 35.0%
AGATATATGGGAGAAGTTTG+TGG - chr7_4:8271970-8271989 Msa1138620:intron 35.0%
AGGAAAAAAGTCAAAAGGGT+AGG - chr7_4:8272104-8272123 Msa1138620:intron 35.0%
GGAAAAAAGTCAAAAGGGTA+GGG - chr7_4:8272105-8272124 Msa1138620:intron 35.0%
GAGATAATGTATGTGGGTAA+AGG + chr7_4:8272147-8272166 None:intergenic 35.0%
AGAAAAAGAATGGAAGACTC+CGG - chr7_4:8272211-8272230 Msa1138620:intron 35.0%
! ATATGTGTGTTTTGCAAACG+TGG - chr7_4:8272443-8272462 Msa1138620:five_prime_UTR 35.0%
! ATGAACCTTCTTGTTTGTTG+TGG - chr7_4:8272572-8272591 Msa1138620:five_prime_UTR 35.0%
TACATCCACAACAAACAAGA+AGG + chr7_4:8272580-8272599 None:intergenic 35.0%
ACAGCAACCAAAAACAAGAA+GGG + chr7_4:8272603-8272622 None:intergenic 35.0%
AACAGCAACCAAAAACAAGA+AGG + chr7_4:8272604-8272623 None:intergenic 35.0%
!! TGGGTTTCTGGAATGATTTG+CGG + chr7_4:8269187-8269206 None:intergenic 40.0%
GATGAGATTCAACCCCAAAT+CGG - chr7_4:8269252-8269271 Msa1138620:three_prime_UTR 40.0%
TGAGGTTGGATTTCCGATTT+GGG + chr7_4:8269268-8269287 None:intergenic 40.0%
TTGAGGTTGGATTTCCGATT+TGG + chr7_4:8269269-8269288 None:intergenic 40.0%
! CAATGATTTGCGGAGAAACA+CGG + chr7_4:8269332-8269351 None:intergenic 40.0%
GAGTCACGAACAATGATTTG+CGG + chr7_4:8269342-8269361 None:intergenic 40.0%
AAAGGTTCGTGACTCTCAAA+TGG - chr7_4:8269379-8269398 Msa1138620:three_prime_UTR 40.0%
AAGGTTCGTGACTCTCAAAT+GGG - chr7_4:8269380-8269399 Msa1138620:three_prime_UTR 40.0%
! GCTCTTTTCTCAAAAGGGTT+TGG - chr7_4:8269431-8269450 Msa1138620:CDS 40.0%
GCTGCTACACAATCATCATA+AGG - chr7_4:8270024-8270043 Msa1138620:intron 40.0%
GCAGGTATGACATTCAAACA+GGG + chr7_4:8270059-8270078 None:intergenic 40.0%
TGCAGGTATGACATTCAAAC+AGG + chr7_4:8270060-8270079 None:intergenic 40.0%
CAATGACCCACAAGAATTCT+AGG - chr7_4:8270383-8270402 Msa1138620:intron 40.0%
GAGAACCTATTTGGGCAATT+CGG + chr7_4:8270424-8270443 None:intergenic 40.0%
AGAATCAGGATGTGTATAGC+AGG + chr7_4:8270608-8270627 None:intergenic 40.0%
TTCACCTTTATCCCACGTAA+TGG - chr7_4:8270634-8270653 Msa1138620:five_prime_UTR 40.0%
TATAGAGAGAGCCATTACGT+GGG + chr7_4:8270648-8270667 None:intergenic 40.0%
ATATAGAGAGAGCCATTACG+TGG + chr7_4:8270649-8270668 None:intergenic 40.0%
!!! TCTTGTGGGGCTGTTTTTTT+GGG - chr7_4:8270883-8270902 Msa1138620:intron 40.0%
!! CTCGCTTTTGTTTGGGATAT+TGG - chr7_4:8271196-8271215 Msa1138620:intron 40.0%
! GCTTATGGTCTCTGTTTTGT+AGG - chr7_4:8271273-8271292 Msa1138620:intron 40.0%
!! TGAAGTTGGTGGAGTCAATA+AGG - chr7_4:8271413-8271432 Msa1138620:intron 40.0%
TTGCTTATCTAGGTAGGTGT+TGG - chr7_4:8271545-8271564 Msa1138620:intron 40.0%
!!! CTAGGTAGGTGTTGGTTTTT+TGG - chr7_4:8271553-8271572 Msa1138620:intron 40.0%
GTACAAATAGCCCAACTGTA+CGG - chr7_4:8271603-8271622 Msa1138620:intron 40.0%
AAACAAACTGCCGTACAGTT+GGG + chr7_4:8271616-8271635 None:intergenic 40.0%
! AGTTTGTTTGAGCCATCAGT+TGG - chr7_4:8271627-8271646 Msa1138620:intron 40.0%
AAAATGTCCCTAAAGCCACT+CGG + chr7_4:8271739-8271758 None:intergenic 40.0%
TCGAAAGACCCCTGAATTTA+AGG - chr7_4:8271997-8272016 Msa1138620:intron 40.0%
TGTATGTGGGTAAAGGTTGT+TGG + chr7_4:8272140-8272159 None:intergenic 40.0%
TACCCACATACATTATCTCG+TGG - chr7_4:8272148-8272167 Msa1138620:intron 40.0%
CTCCACGAGATAATGTATGT+GGG + chr7_4:8272153-8272172 None:intergenic 40.0%
TTCCATTTGTCATGACGTGA+CGG + chr7_4:8272262-8272281 None:intergenic 40.0%
! AAGAGAGCACGACAAAAGAA+AGG - chr7_4:8272280-8272299 Msa1138620:intron 40.0%
! AGAGAGCACGACAAAAGAAA+GGG - chr7_4:8272281-8272300 Msa1138620:intron 40.0%
! ACGTGGTACTGAAGATATGT+TGG - chr7_4:8272460-8272479 Msa1138620:five_prime_UTR 40.0%
!!! GGATGTACCCTTCTTGTTTT+TGG - chr7_4:8272593-8272612 Msa1138620:five_prime_UTR 40.0%
! AGCTTTGTGGTGAGAGTGAA+CGG + chr7_4:8269156-8269175 None:intergenic 45.0%
! TCTGGAATGATTTGCGGATC+TGG + chr7_4:8269181-8269200 None:intergenic 45.0%
TGGGGTTGAATCTCATCTCA+TGG + chr7_4:8269249-8269268 None:intergenic 45.0%
! GAGGTTGGATTTCCGATTTG+GGG + chr7_4:8269267-8269286 None:intergenic 45.0%
! AACGAGAGGAGATTTGAGGT+TGG + chr7_4:8269282-8269301 None:intergenic 45.0%
GAGTAACAATCACGAACGAG+AGG + chr7_4:8269296-8269315 None:intergenic 45.0%
TCTCAAGGTCTGACTACTAC+TGG + chr7_4:8269862-8269881 None:intergenic 45.0%
TGCTTCATTGAGGCAAAGTG+AGG + chr7_4:8269918-8269937 None:intergenic 45.0%
!! TTTTGGGGATCAGATGCCAA+AGG - chr7_4:8269950-8269969 Msa1138620:five_prime_UTR 45.0%
TTTGGGGATCAGATGCCAAA+GGG - chr7_4:8269951-8269970 Msa1138620:five_prime_UTR 45.0%
ATTTGGGCAATTCGGTCAAG+AGG + chr7_4:8270416-8270435 None:intergenic 45.0%
CTTGACCGAATTGCCCAAAT+AGG - chr7_4:8270416-8270435 Msa1138620:intron 45.0%
TGAACTCTGACACTCATCCT+TGG - chr7_4:8270468-8270487 Msa1138620:intron 45.0%
AAATGAATATGTCCTCGGGG+AGG - chr7_4:8270506-8270525 Msa1138620:intron 45.0%
AATTACTAAGATCCTCCCCG+AGG + chr7_4:8270521-8270540 None:intergenic 45.0%
AAGGTGAAGTCTCGAGAATC+AGG + chr7_4:8270622-8270641 None:intergenic 45.0%
AGAGCCATTACGTGGGATAA+AGG + chr7_4:8270641-8270660 None:intergenic 45.0%
TGGAACCCAAGAGATTGTCT+TGG - chr7_4:8270831-8270850 Msa1138620:intron 45.0%
!!! AATGGGGTTTTTGGCTCTTG+TGG - chr7_4:8270868-8270887 Msa1138620:intron 45.0%
!!! ATGGGGTTTTTGGCTCTTGT+GGG - chr7_4:8270869-8270888 Msa1138620:intron 45.0%
!!! CTCTTGTGGGGCTGTTTTTT+TGG - chr7_4:8270882-8270901 Msa1138620:intron 45.0%
!!! GGGCTGTTTTTTTGGGACTA+TGG - chr7_4:8270890-8270909 Msa1138620:intron 45.0%
AAAAAATGACCTGTCCAGGG+AGG + chr7_4:8270986-8271005 None:intergenic 45.0%
!! AGTTGGTGGAGTCAATAAGG+AGG - chr7_4:8271416-8271435 Msa1138620:intron 45.0%
!!! GGTTTTGTTTGACGCGGTAA+AGG - chr7_4:8271443-8271462 Msa1138620:intron 45.0%
! TAAAGGCCTTATCTTGGTGC+CGG - chr7_4:8271460-8271479 Msa1138620:intron 45.0%
CAAACAAACTGCCGTACAGT+TGG + chr7_4:8271617-8271636 None:intergenic 45.0%
AGAGACATAGAAACAGCAGC+AGG + chr7_4:8271672-8271691 None:intergenic 45.0%
GGATCTGGATCATCACTTTC+TGG + chr7_4:8271806-8271825 None:intergenic 45.0%
GTGTCTTAAAACCTCTCCAC+CGG + chr7_4:8271874-8271893 None:intergenic 45.0%
CCTCCACGAGATAATGTATG+TGG + chr7_4:8272154-8272173 None:intergenic 45.0%
CCACATACATTATCTCGTGG+AGG - chr7_4:8272151-8272170 Msa1138620:intron 45.0%
! GAGAGCACGACAAAAGAAAG+GGG - chr7_4:8272282-8272301 Msa1138620:intron 45.0%
AGAGCACGACAAAAGAAAGG+GGG - chr7_4:8272283-8272302 Msa1138620:intron 45.0%
!! TTAACTATATATTAATTAAT+TGG - chr7_4:8269766-8269785 Msa1138620:intron 5.0%
TTTGTGGTGAGAGGGGTAGA+GGG + chr7_4:8269099-8269118 None:intergenic 50.0%
! TGGAGAGCTTTGTGGTGAGA+GGG + chr7_4:8269107-8269126 None:intergenic 50.0%
ACTGGATCTGGAGAGCTTTG+TGG + chr7_4:8269115-8269134 None:intergenic 50.0%
TGGTGAGAGTGAACTGGATC+TGG + chr7_4:8269127-8269146 None:intergenic 50.0%
TTTGTGGTGAGAGTGAACGG+CGG + chr7_4:8269153-8269172 None:intergenic 50.0%
CACGAACGAGAGGAGATTTG+AGG + chr7_4:8269286-8269305 None:intergenic 50.0%
! TGACTACTACTGGTGGTGCA+TGG + chr7_4:8269852-8269871 None:intergenic 50.0%
CAAGGTCTGACTACTACTGG+TGG + chr7_4:8269859-8269878 None:intergenic 50.0%
!!! TGGGGTTTTTGGCTCTTGTG+GGG - chr7_4:8270870-8270889 Msa1138620:intron 50.0%
TGGTGGAGTCAATAAGGAGG+CGG - chr7_4:8271419-8271438 Msa1138620:intron 50.0%
! ACGCGGTAAAGGCCTTATCT+TGG - chr7_4:8271454-8271473 Msa1138620:intron 50.0%
AAGACACCGGCACCAAGATA+AGG + chr7_4:8271469-8271488 None:intergenic 50.0%
! GATGACGGCAGTCTTTGGAA+GGG + chr7_4:8271922-8271941 None:intergenic 50.0%
! AAGTGGATGACGGCAGTCTT+TGG + chr7_4:8271927-8271946 None:intergenic 50.0%
CATTTGTCATGACGTGACGG+CGG + chr7_4:8272259-8272278 None:intergenic 50.0%
AGGGGGAGAATACACAACAG+AGG - chr7_4:8272300-8272319 Msa1138620:intron 50.0%
TGAGAGGGGTAGAGGGTTTG+TGG + chr7_4:8269092-8269111 None:intergenic 55.0%
CTTTGTGGTGAGAGGGGTAG+AGG + chr7_4:8269100-8269119 None:intergenic 55.0%
! GGAGAGCTTTGTGGTGAGAG+GGG + chr7_4:8269106-8269125 None:intergenic 55.0%
! CTGGAGAGCTTTGTGGTGAG+AGG + chr7_4:8269108-8269127 None:intergenic 55.0%
CGGATCTGGTGAGAGTGAAC+TGG + chr7_4:8269133-8269152 None:intergenic 55.0%
TGCGGATCTGGAGAGCTTTG+TGG + chr7_4:8269169-8269188 None:intergenic 55.0%
!! GTGAGCTTGCTGAGCTTGCA+AGG - chr7_4:8270348-8270367 Msa1138620:intron 55.0%
TGGGCAATTCGGTCAAGAGG+TGG + chr7_4:8270413-8270432 None:intergenic 55.0%
TGGAGTCAATAAGGAGGCGG+AGG - chr7_4:8271422-8271441 Msa1138620:intron 55.0%
!!! GGCGGAGGTTTTGTTTGACG+CGG - chr7_4:8271437-8271456 Msa1138620:intron 55.0%
!!! GGTTTTTTGGTGCAGCTGCC+CGG - chr7_4:8271566-8271585 Msa1138620:intron 55.0%
! TTTTTCTGCTGCTGGCCGAG+TGG - chr7_4:8271721-8271740 Msa1138620:intron 55.0%
! GGATGATGGTTTGTGGGGAC+CGG - chr7_4:8271852-8271871 Msa1138620:five_prime_UTR 55.0%
! GGATGACGGCAGTCTTTGGA+AGG + chr7_4:8271923-8271942 None:intergenic 55.0%
CGCCGTCACGTCATGACAAA+TGG - chr7_4:8272257-8272276 Msa1138620:intron 55.0%
GTGAGAGTGAACGGCGGATC+TGG + chr7_4:8269147-8269166 None:intergenic 60.0%
GTCATTGTGCCTCCCTGGAC+AGG - chr7_4:8270974-8270993 Msa1138620:intron 60.0%
TGTACGCCGCACACCAAAGC+CGG + chr7_4:8271588-8271607 None:intergenic 60.0%
! CTGCTGGCCGAGTGGCTTTA+GGG - chr7_4:8271729-8271748 Msa1138620:intron 60.0%
! TGATGGTTTGTGGGGACCGG+TGG - chr7_4:8271855-8271874 Msa1138620:five_prime_UTR 60.0%
! GGCGTGTCATTGTGCCTCCC+TGG - chr7_4:8270969-8270988 Msa1138620:intron 65.0%
TTGGTGCAGCTGCCCGGCTT+TGG - chr7_4:8271572-8271591 Msa1138620:intron 65.0%
! AGCTGCCCGGCTTTGGTGTG+CGG - chr7_4:8271579-8271598 Msa1138620:intron 65.0%
GTACGCCGCACACCAAAGCC+GGG + chr7_4:8271587-8271606 None:intergenic 65.0%
GCTGCTGGCCGAGTGGCTTT+AGG - chr7_4:8271728-8271747 Msa1138620:intron 65.0%
GTTTGTGGGGACCGGTGGAG+AGG - chr7_4:8271860-8271879 Msa1138620:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 8269083 8272711 8269083 ID=Msa1138620;Name=Msa1138620
chr7_4 mRNA 8269083 8272711 8269083 ID=Msa1138620-mRNA-1;Parent=Msa1138620;Name=Msa1138620-mRNA-1;_AED=0.15;_eAED=0.15;_QI=594|1|1|1|0.25|0.6|5|333|145
chr7_4 exon 8269083 8269688 8269083 ID=Msa1138620-mRNA-1:exon:2244;Parent=Msa1138620-mRNA-1
chr7_4 exon 8269776 8270006 8269776 ID=Msa1138620-mRNA-1:exon:2243;Parent=Msa1138620-mRNA-1
chr7_4 exon 8270616 8270693 8270616 ID=Msa1138620-mRNA-1:exon:2242;Parent=Msa1138620-mRNA-1
chr7_4 exon 8271822 8271971 8271822 ID=Msa1138620-mRNA-1:exon:2241;Parent=Msa1138620-mRNA-1
chr7_4 exon 8272412 8272711 8272412 ID=Msa1138620-mRNA-1:exon:2240;Parent=Msa1138620-mRNA-1
chr7_4 five_prime_UTR 8272412 8272711 8272412 ID=Msa1138620-mRNA-1:five_prime_utr;Parent=Msa1138620-mRNA-1
chr7_4 five_prime_UTR 8271822 8271971 8271822 ID=Msa1138620-mRNA-1:five_prime_utr;Parent=Msa1138620-mRNA-1
chr7_4 five_prime_UTR 8270616 8270693 8270616 ID=Msa1138620-mRNA-1:five_prime_utr;Parent=Msa1138620-mRNA-1
chr7_4 five_prime_UTR 8269941 8270006 8269941 ID=Msa1138620-mRNA-1:five_prime_utr;Parent=Msa1138620-mRNA-1
chr7_4 CDS 8269776 8269940 8269776 ID=Msa1138620-mRNA-1:cds;Parent=Msa1138620-mRNA-1
chr7_4 CDS 8269416 8269688 8269416 ID=Msa1138620-mRNA-1:cds;Parent=Msa1138620-mRNA-1
chr7_4 three_prime_UTR 8269083 8269415 8269083 ID=Msa1138620-mRNA-1:three_prime_utr;Parent=Msa1138620-mRNA-1
Gene Sequence

>Msa1138620

ATGATGGTTTGTGGGGACCGGTGGAGAGGTTTTAAGACACAACTCACAAATGATTATATTACAAACCCTTCCAAAGACTGCCGTCATCCACTTGTAAAGTATCCTTTTCTTCAAAAAGATATATGGGAGAAGTTTGTGGAGTCTCGAAAGACCCCTGAATTTAAGGAAAAAAGTCAAAAGGGTAGGGATACTGTTGCCAACAACCTTTACCCACATACATTATCTCGTGGAGGATATGATTACCTTGAAGATAAAATGATGGATGAAAAGAGAAAAAGAATGGAAGACTCCGGAGAATTTGATCATATTCTATCTCCGCCGTCACGTCATGACAAATGGAAGAGAGCACGACAAAAGAAAGGGGGAGAATACACAACAGAGGCTACACGAGTTGTTGCTGAGAAAATTGTAAGTAATATGAGTAATGAGTCTTATTAA
Protein sequence

>Msa1138620

MMVCGDRWRGFKTQLTNDYITNPSKDCRHPLVKYPFLQKDIWEKFVESRKTPEFKEKSQKGRDTVANNLYPHTLSRGGYDYLEDKMMDEKRKRMEDSGEFDHILSPPSRHDKWKRARQKKGGEYTTEATRVVAEKIVSNMSNESY*