AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0032620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0032620 MtrunA17_Chr6g0469071 62.676 142 47 1 1 136 113 254 9.95e-55 181
Msa0032620 MtrunA17_Chr1g0153901 62.238 143 47 2 1 136 113 255 2.99e-54 178
Msa0032620 MtrunA17_Chr2g0325841 44.526 137 73 2 4 138 600 735 4.11e-31 117
Msa0032620 MtrunA17_Chr4g0010671 45.833 144 76 2 1 142 111 254 8.85e-29 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAAATGTTTATGGATTT+TGG 0.040149 1_1:-59789238 None:intergenic
AAGCAAAATTGTTTCTATTT+TGG 0.049224 1_1:+59789950 Msa0032620:five_prime_UTR
ATGGAATTTCTAAATGTTTA+TGG 0.103894 1_1:-59789245 None:intergenic
TCGAAAGACCCCTGAATTTA+AGG 0.126971 1_1:+59792015 Msa0032620:CDS
GGATCTGGATCATCACTTTC+TGG 0.146392 1_1:-59791821 None:intergenic
TTTCTGAAGATTTGGGTTTC+TGG 0.158057 1_1:-59789214 None:intergenic
CTTCCTTCTTCAAAACAAAA+AGG 0.224595 1_1:+59789379 Msa0032620:five_prime_UTR
ATGTAATTTGGATAAGTAAT+TGG 0.254965 1_1:+59792438 Msa0032620:three_prime_UTR
GTGTGTTTGGATATGTAATT+TGG 0.260929 1_1:+59792426 Msa0032620:three_prime_UTR
TTGAGGTTGGATTTCCGATT+TGG 0.283215 1_1:-59789284 None:intergenic
TGAGGTTGGATTTCCGATTT+GGG 0.291966 1_1:-59789283 None:intergenic
TGCATGGATTCCTGAATTTA+TGG 0.314614 1_1:-59789850 None:intergenic
ATCACTTCCTATTTGTGCTT+TGG 0.318322 1_1:+59791141 Msa0032620:five_prime_UTR
TGAGAGGGGTAGAGGGTTTG+TGG 0.336486 1_1:-59789107 None:intergenic
ACACAAAACTTTGTTATCAA+TGG 0.339705 1_1:-59791171 None:intergenic
CGTATACTAACCTTAAATTC+AGG 0.342289 1_1:-59792025 None:intergenic
CTTTGTGGTGAGAGGGGTAG+AGG 0.345291 1_1:-59789115 None:intergenic
TCTGGAATGATTTGCGGATC+TGG 0.365385 1_1:-59789196 None:intergenic
AAGTGGATGACGGCAGTCTT+TGG 0.369068 1_1:-59791942 None:intergenic
TGTAGTTTGACCATAAATTC+AGG 0.371579 1_1:+59789840 Msa0032620:five_prime_UTR
GAGATTTCTAGCATTTGTTC+AGG 0.377979 1_1:+59789895 Msa0032620:five_prime_UTR
CGCCGTCACGTGATGACAAA+TGG 0.380342 1_1:+59792275 Msa0032620:CDS
TGGTGAGAGTGAACTGGATC+TGG 0.391142 1_1:-59789142 None:intergenic
GTGAGAGTGAACGGCGAATC+TGG 0.429587 1_1:-59789162 None:intergenic
AACGAAACCAAAGCACAAAT+AGG 0.430455 1_1:-59791148 None:intergenic
ATAAATAGATGATGTGTGTT+TGG 0.432806 1_1:+59792413 Msa0032620:three_prime_UTR
AGATATATGGGAGAAGTTTG+TGG 0.470943 1_1:+59791988 Msa0032620:CDS
ATGAACCTTCTTGTTTGTTG+TGG 0.474107 1_1:+59792590 Msa0032620:three_prime_UTR
GGATGATGGTTTGTGGGGAC+CGG 0.476464 1_1:+59791870 Msa0032620:exon
CGAATCTGGTGAGAGTGAAC+TGG 0.479191 1_1:-59789148 None:intergenic
CTGGAGAGCTTTGTGGTGAG+AGG 0.482427 1_1:-59789123 None:intergenic
TCTCAAGGTCTGACTACTAC+TGG 0.483198 1_1:-59789876 None:intergenic
TGCGGATCTGGAGAGCTTTG+TGG 0.486766 1_1:-59789184 None:intergenic
ACTGGATCTGGAGAGCTTTG+TGG 0.493467 1_1:-59789130 None:intergenic
CCTCCACGAGATAATGTATG+TGG 0.495099 1_1:-59792169 None:intergenic
TTTGTGGTGAGAGGGGTAGA+GGG 0.497534 1_1:-59789114 None:intergenic
CACGAACGAGAGGAGATTTG+AGG 0.499345 1_1:-59789301 None:intergenic
TGGGTTTCTGGAATGATTTG+CGG 0.506631 1_1:-59789202 None:intergenic
AGAGAGCACGACAAAAGAAA+GGG 0.507249 1_1:+59792299 Msa0032620:CDS
GATGAGATTCAACCCCAAAT+CGG 0.508707 1_1:+59789270 Msa0032620:five_prime_UTR
AGCTTTGTGGTGAGAGTGAA+CGG 0.517782 1_1:-59789171 None:intergenic
TAGAATATGATCAAATTCTC+CGG 0.517940 1_1:-59792248 None:intergenic
TGGGGTTGAATCTCATCTCA+TGG 0.519668 1_1:-59789264 None:intergenic
GGATGACGGCAGTCTTTGGA+AGG 0.520877 1_1:-59791938 None:intergenic
AAGAGAGCACGACAAAAGAA+AGG 0.521144 1_1:+59792298 Msa0032620:CDS
GTATACTAACCTTAAATTCA+GGG 0.523147 1_1:-59792024 None:intergenic
GAGATAATGTATGTGGGTAA+AGG 0.527971 1_1:-59792162 None:intergenic
CAATGATTTGCGGAGAAACA+CGG 0.529179 1_1:-59789347 None:intergenic
TGTATGTGGGTAAAGGTTGT+TGG 0.530757 1_1:-59792155 None:intergenic
TGACTACTACTGGTGGTGCA+TGG 0.530917 1_1:-59789866 None:intergenic
TGATGGTTTGTGGGGACCGG+TGG 0.532136 1_1:+59791873 Msa0032620:CDS
AACGAGAGGAGATTTGAGGT+TGG 0.542755 1_1:-59789297 None:intergenic
GTTTGTGGGGACCGGTGGAG+AGG 0.557784 1_1:+59791878 Msa0032620:CDS
TGGAGAGCTTTGTGGTGAGA+GGG 0.558180 1_1:-59789122 None:intergenic
AACAAATGCTAGAAATCTCA+AGG 0.561817 1_1:-59789891 None:intergenic
TTACCTTGAAGATAAAATGA+TGG 0.575253 1_1:+59792198 Msa0032620:CDS
AGAAAAGGATACTTTACAAG+TGG 0.577527 1_1:-59791959 None:intergenic
TTTGGGGATCAGATGCCAAA+GGG 0.580047 1_1:+59789968 Msa0032620:five_prime_UTR
GAGTCACGAACAATGATTTG+CGG 0.583640 1_1:-59789357 None:intergenic
TTCCATTTGTCATCACGTGA+CGG 0.595200 1_1:-59792277 None:intergenic
GTGTCTTAAAACCTCTCCAC+CGG 0.595488 1_1:-59791889 None:intergenic
TACATCCACAACAAACAAGA+AGG 0.605162 1_1:-59792595 None:intergenic
TGCTTCATGGAGGCAAAGTG+AGG 0.605187 1_1:-59789932 None:intergenic
TACCCACATACATTATCTCG+TGG 0.605189 1_1:+59792166 Msa0032620:CDS
GAGAGCACGACAAAAGAAAG+GGG 0.611004 1_1:+59792300 Msa0032620:CDS
ACGTGGTACTGAAGATATGT+TGG 0.612925 1_1:+59792478 Msa0032620:three_prime_UTR
TATACTAACCTTAAATTCAG+GGG 0.621254 1_1:-59792023 None:intergenic
GATGACGGCAGTCTTTGGAA+GGG 0.627217 1_1:-59791937 None:intergenic
GGAGAGCTTTGTGGTGAGAG+GGG 0.630932 1_1:-59789121 None:intergenic
GAGGTTGGATTTCCGATTTG+GGG 0.646354 1_1:-59789282 None:intergenic
CTCCACGAGATAATGTATGT+GGG 0.647609 1_1:-59792168 None:intergenic
GATACTTTACAAGTGGATGA+CGG 0.655215 1_1:-59791952 None:intergenic
AGAGCACGACAAAAGAAAGG+GGG 0.662182 1_1:+59792301 Msa0032620:CDS
GAGTAACAATCACGAACGAG+AGG 0.668417 1_1:-59789311 None:intergenic
CATTTGTCATCACGTGACGG+CGG 0.673401 1_1:-59792274 None:intergenic
CAAGGTCTGACTACTACTGG+TGG 0.680676 1_1:-59789873 None:intergenic
CCACATACATTATCTCGTGG+AGG 0.684741 1_1:+59792169 Msa0032620:CDS
AAAATGGATGATGGTTTGTG+GGG 0.689791 1_1:+59791865 Msa0032620:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTATGTTTTTTGAAA+GGG + chr1_1:59789516-59789535 Msa0032620:intron 10.0%
!!! TAATGAATTTGATTTATAAT+AGG + chr1_1:59792102-59792121 Msa0032620:intron 10.0%
!!! TAAATTTCTCTTTTTTCAAA+TGG + chr1_1:59789475-59789494 Msa0032620:intron 15.0%
!!! AAATTTCTCTTTTTTCAAAT+GGG + chr1_1:59789476-59789495 Msa0032620:intron 15.0%
!!! GAATTTTTATGTTTTTTGAA+AGG + chr1_1:59789515-59789534 Msa0032620:intron 15.0%
!!! ATTTTTATGTTTTTTGAAAG+GGG + chr1_1:59789517-59789536 Msa0032620:intron 15.0%
!! ATATTAGTAATATTCTTCAT+GGG - chr1_1:59789670-59789689 None:intergenic 15.0%
!! AATATTAGTAATATTCTTCA+TGG - chr1_1:59789671-59789690 None:intergenic 15.0%
!!! TAATCTTCTATTTGTAATTT+TGG + chr1_1:59789991-59790010 Msa0032620:intron 15.0%
!!! AATCTTCTATTTGTAATTTT+GGG + chr1_1:59789992-59790011 Msa0032620:intron 15.0%
!! GAACAAATCAAATTTAATAT+TGG - chr1_1:59790820-59790839 None:intergenic 15.0%
!! AATAAATAATAACACAAACA+TGG - chr1_1:59791323-59791342 None:intergenic 15.0%
!! TGTCTTAATAGATTAAAAAT+TGG + chr1_1:59791503-59791522 Msa0032620:intron 15.0%
!!! TTTTCTTCAAAAAGATATAT+GGG + chr1_1:59791976-59791995 Msa0032620:CDS 15.0%
!! TAAACAATTAAATTAGTACA+GGG - chr1_1:59792068-59792087 None:intergenic 15.0%
!! ATAAACAATTAAATTAGTAC+AGG - chr1_1:59792069-59792088 None:intergenic 15.0%
!!! TTCTAAATGTTTATGGATTT+TGG - chr1_1:59789241-59789260 None:intergenic 20.0%
!! ATGGAATTTCTAAATGTTTA+TGG - chr1_1:59789248-59789267 None:intergenic 20.0%
!!! TTTTTCTTCTTCTTTCAAAT+TGG + chr1_1:59789550-59789569 Msa0032620:intron 20.0%
!!! TTTTCTTCTTCTTTCAAATT+GGG + chr1_1:59789551-59789570 Msa0032620:intron 20.0%
!!! AAGCAAAATTGTTTCTATTT+TGG + chr1_1:59789950-59789969 Msa0032620:five_prime_UTR 20.0%
!!! AGCAAAATTGTTTCTATTTT+GGG + chr1_1:59789951-59789970 Msa0032620:five_prime_UTR 20.0%
!!! ATTAGTCTTATTATATATGC+AGG - chr1_1:59790094-59790113 None:intergenic 20.0%
!! CAATATCAATATTCTTTCAA+TGG - chr1_1:59790463-59790482 None:intergenic 20.0%
!! GATTGTTATATTAGTATTAG+TGG + chr1_1:59790765-59790784 Msa0032620:intron 20.0%
!!! TTTGATTTGTTCTTTTATGT+TGG + chr1_1:59790828-59790847 Msa0032620:intron 20.0%
!!! TTGGTAGATAAGTTTTTTAA+TGG + chr1_1:59790867-59790886 Msa0032620:intron 20.0%
!!! TGGTAGATAAGTTTTTTAAT+GGG + chr1_1:59790868-59790887 Msa0032620:intron 20.0%
!!! ATAACAAAGTTTTGTGTTTA+AGG + chr1_1:59791177-59791196 Msa0032620:five_prime_UTR 20.0%
!! TAATTAAAGTCATTTGCTTA+TGG + chr1_1:59791278-59791297 Msa0032620:intron 20.0%
!!! TTTTTAATCTATTAAGACAC+CGG - chr1_1:59791502-59791521 None:intergenic 20.0%
!!! TTTTTCAAAGCATCTTTTTT+TGG - chr1_1:59791845-59791864 None:intergenic 20.0%
!!! AAGATGCTTTGAAAAAAAAA+TGG + chr1_1:59791849-59791868 Msa0032620:five_prime_UTR 20.0%
!! TTTGAAAAAAAAATGGATGA+TGG + chr1_1:59791856-59791875 Msa0032620:exon 20.0%
!!! CATATATCTTTTTGAAGAAA+AGG - chr1_1:59791977-59791996 None:intergenic 20.0%
!!! CTTTTCTTCAAAAAGATATA+TGG + chr1_1:59791975-59791994 Msa0032620:CDS 20.0%
!! ATAATAGGAAAAAAGTCAAA+AGG + chr1_1:59792117-59792136 Msa0032620:intron 20.0%
!! TAATAGGAAAAAAGTCAAAA+GGG + chr1_1:59792118-59792137 Msa0032620:intron 20.0%
!! ATGTAATTTGGATAAGTAAT+TGG + chr1_1:59792438-59792457 Msa0032620:three_prime_UTR 20.0%
!!! TTTTGTTTGATTTAGAAGAA+TGG + chr1_1:59792661-59792680 Msa0032620:three_prime_UTR 20.0%
!!! TTTTGGATTTCTGAAGATTT+GGG - chr1_1:59789224-59789243 None:intergenic 25.0%
!!! ATTTTGGATTTCTGAAGATT+TGG - chr1_1:59789225-59789244 None:intergenic 25.0%
!!! TTTTTTGCTCTTTTCTCAAA+AGG + chr1_1:59789443-59789462 Msa0032620:intron 25.0%
!! TTTTTGCTCTTTTCTCAAAA+GGG + chr1_1:59789444-59789463 Msa0032620:intron 25.0%
! AAGTATACATATGATACACT+TGG + chr1_1:59789705-59789724 Msa0032620:intron 25.0%
!! ATAGAAACAATTTTGCTTCA+TGG - chr1_1:59789948-59789967 None:intergenic 25.0%
!!! GCAAAATTGTTTCTATTTTG+GGG + chr1_1:59789952-59789971 Msa0032620:five_prime_UTR 25.0%
! TTGTATCAAAATAGAATCCA+AGG - chr1_1:59790505-59790524 None:intergenic 25.0%
! GATTTAGTAGAAAATTGTTG+AGG - chr1_1:59790579-59790598 None:intergenic 25.0%
! ATGCCTATCAATTGTTATAT+TGG + chr1_1:59790696-59790715 Msa0032620:intron 25.0%
! TGCCTATCAATTGTTATATT+GGG + chr1_1:59790697-59790716 Msa0032620:intron 25.0%
! ATCCCAATATAACAATTGAT+AGG - chr1_1:59790702-59790721 None:intergenic 25.0%
!!! GGTAGATAAGTTTTTTAATG+GGG + chr1_1:59790869-59790888 Msa0032620:intron 25.0%
!!! AAGTTTTTTAATGGGGTTTT+TGG + chr1_1:59790876-59790895 Msa0032620:intron 25.0%
! TCCTATTTATTGTTTGTTCT+TGG + chr1_1:59790964-59790983 Msa0032620:intron 25.0%
! CCTATTTATTGTTTGTTCTT+GGG + chr1_1:59790965-59790984 Msa0032620:intron 25.0%
!! ACACAAAACTTTGTTATCAA+TGG - chr1_1:59791174-59791193 None:intergenic 25.0%
!!! CAATGTTTTTAACTATAGCA+GGG - chr1_1:59791389-59791408 None:intergenic 25.0%
!!! TCAATGTTTTTAACTATAGC+AGG - chr1_1:59791390-59791409 None:intergenic 25.0%
! TTTAACTAACAACATGAAGT+TGG + chr1_1:59791419-59791438 Msa0032620:intron 25.0%
! TATACTAACCTTAAATTCAG+GGG - chr1_1:59792026-59792045 None:intergenic 25.0%
! GTATACTAACCTTAAATTCA+GGG - chr1_1:59792027-59792046 None:intergenic 25.0%
! TTACCTTGAAGATAAAATGA+TGG + chr1_1:59792198-59792217 Msa0032620:CDS 25.0%
! TAGAATATGATCAAATTCTC+CGG - chr1_1:59792251-59792270 None:intergenic 25.0%
! ATAAATAGATGATGTGTGTT+TGG + chr1_1:59792413-59792432 Msa0032620:three_prime_UTR 25.0%
CTTCCTTCTTCAAAACAAAA+AGG + chr1_1:59789379-59789398 Msa0032620:five_prime_UTR 30.0%
!!! GAACCTTTTTGTTTTGAAGA+AGG - chr1_1:59789385-59789404 None:intergenic 30.0%
!!! TCTCTTTTTTCAAATGGGTT+TGG + chr1_1:59789481-59789500 Msa0032620:intron 30.0%
!!! CTCTTTTTTCAAATGGGTTT+GGG + chr1_1:59789482-59789501 Msa0032620:intron 30.0%
TGTAGTTTGACCATAAATTC+AGG + chr1_1:59789840-59789859 Msa0032620:five_prime_UTR 30.0%
AACAAATGCTAGAAATCTCA+AGG - chr1_1:59789894-59789913 None:intergenic 30.0%
ACAAATAGAAGATTACCCTT+TGG - chr1_1:59789986-59790005 None:intergenic 30.0%
CAATGAGTCTCTATATGAAA+AGG - chr1_1:59790022-59790041 None:intergenic 30.0%
ACATAACACAAGCATATCTT+GGG - chr1_1:59790339-59790358 None:intergenic 30.0%
TACATAACACAAGCATATCT+TGG - chr1_1:59790340-59790359 None:intergenic 30.0%
GATACAAATGAATATGTCCT+CGG + chr1_1:59790518-59790537 Msa0032620:intron 30.0%
TTAGTAGAAAATTGTTGAGG+TGG - chr1_1:59790576-59790595 None:intergenic 30.0%
TGTATTTCATTGTTAGTGGA+AGG + chr1_1:59790934-59790953 Msa0032620:intron 30.0%
CCCAAGAACAAACAATAAAT+AGG - chr1_1:59790968-59790987 None:intergenic 30.0%
AAAAAAAAATGACCTGTCCA+GGG - chr1_1:59791006-59791025 None:intergenic 30.0%
AAAAAAAAAATGACCTGTCC+AGG - chr1_1:59791007-59791026 None:intergenic 30.0%
AACAAAAAGCGATCATTAAG+AGG - chr1_1:59791090-59791109 None:intergenic 30.0%
! GTGCTTTTATGTTCTATGAA+TGG + chr1_1:59791525-59791544 Msa0032620:intron 30.0%
TTATGTTCTATGAATGGTGT+TGG + chr1_1:59791531-59791550 Msa0032620:intron 30.0%
!!! AAAGCATCTTTTTTTGGATC+TGG - chr1_1:59791839-59791858 None:intergenic 30.0%
! AAAAAATGGATGATGGTTTG+TGG + chr1_1:59791863-59791882 Msa0032620:exon 30.0%
! AAAAATGGATGATGGTTTGT+GGG + chr1_1:59791864-59791883 Msa0032620:exon 30.0%
AGAAAAGGATACTTTACAAG+TGG - chr1_1:59791962-59791981 None:intergenic 30.0%
CGTATACTAACCTTAAATTC+AGG - chr1_1:59792028-59792047 None:intergenic 30.0%
! GAATTTAAGGTTAGTATACG+TGG + chr1_1:59792028-59792047 Msa0032620:intron 30.0%
! CATCCATCATTTTATCTTCA+AGG - chr1_1:59792204-59792223 None:intergenic 30.0%
GGATGAAAAGAGAAAAAGAA+TGG + chr1_1:59792219-59792238 Msa0032620:CDS 30.0%
GTGTGTTTGGATATGTAATT+TGG + chr1_1:59792426-59792445 Msa0032620:three_prime_UTR 30.0%
!!! GAGAGACCTTTTTTTTGTTT+TGG + chr1_1:59792511-59792530 Msa0032620:three_prime_UTR 30.0%
GCAGTACCAAAACAAAAAAA+AGG - chr1_1:59792520-59792539 None:intergenic 30.0%
!!! TTTTTTTGTTTTGGTACTGC+TGG + chr1_1:59792520-59792539 Msa0032620:three_prime_UTR 30.0%
!!! TTTTTTGTTTTGGTACTGCT+GGG + chr1_1:59792521-59792540 Msa0032620:three_prime_UTR 30.0%
! TGTTGACATAGACATTTTGA+AGG + chr1_1:59792638-59792657 Msa0032620:three_prime_UTR 30.0%
! TTTCTGAAGATTTGGGTTTC+TGG - chr1_1:59789217-59789236 None:intergenic 35.0%
!! TGACTCTCAAATGGGTTTTT+AGG + chr1_1:59789406-59789425 Msa0032620:intron 35.0%
!! GACTCTCAAATGGGTTTTTA+GGG + chr1_1:59789407-59789426 Msa0032620:intron 35.0%
!!! TCAAATGGGTTTTTAGGGTT+AGG + chr1_1:59789412-59789431 Msa0032620:intron 35.0%
! CTCTTTTCTCAAAAGGGTTT+GGG + chr1_1:59789450-59789469 Msa0032620:intron 35.0%
! TCAAATGGGTTTGGGTTTAA+AGG + chr1_1:59789490-59789509 Msa0032620:intron 35.0%
CTAATGAAACTAGCTCAGTT+TGG + chr1_1:59789742-59789761 Msa0032620:intron 35.0%
TGCATGGATTCCTGAATTTA+TGG - chr1_1:59789853-59789872 None:intergenic 35.0%
!! GAGATTTCTAGCATTTGTTC+AGG + chr1_1:59789895-59789914 Msa0032620:five_prime_UTR 35.0%
! GAAACAATTTTGCTTCATGG+AGG - chr1_1:59789945-59789964 None:intergenic 35.0%
GGAAAACCTAGAATTCTTGT+GGG - chr1_1:59790409-59790428 None:intergenic 35.0%
TGGAAAACCTAGAATTCTTG+TGG - chr1_1:59790410-59790429 None:intergenic 35.0%
GAGAACCTATTTGGACAATT+CGG - chr1_1:59790441-59790460 None:intergenic 35.0%
CTTTCAATGGAGAACCTATT+TGG - chr1_1:59790450-59790469 None:intergenic 35.0%
CAGGACAACATGTGAAATAT+AGG - chr1_1:59790606-59790625 None:intergenic 35.0%
ATCTACCAAGACAATCTCTT+GGG - chr1_1:59790856-59790875 None:intergenic 35.0%
TATCTACCAAGACAATCTCT+TGG - chr1_1:59790857-59790876 None:intergenic 35.0%
CCACTAACAATGAAATACAG+AGG - chr1_1:59790933-59790952 None:intergenic 35.0%
CCTCTGTATTTCATTGTTAG+TGG + chr1_1:59790930-59790949 Msa0032620:intron 35.0%
!!! CGCTTTTTGTTTTCATTTGC+AGG + chr1_1:59791099-59791118 Msa0032620:intron 35.0%
ATCACTTCCTATTTGTGCTT+TGG + chr1_1:59791141-59791160 Msa0032620:five_prime_UTR 35.0%
AACGAAACCAAAGCACAAAT+AGG - chr1_1:59791151-59791170 None:intergenic 35.0%
!! TTTTCTCTCTCGCTTTTGTT+TGG + chr1_1:59791208-59791227 Msa0032620:intron 35.0%
!! TTTCTCTCTCGCTTTTGTTT+GGG + chr1_1:59791209-59791228 Msa0032620:intron 35.0%
AACTAACAACATGAAGTTGG+TGG + chr1_1:59791422-59791441 Msa0032620:intron 35.0%
ATCCTAGAGATTGCTTATCT+AGG + chr1_1:59791555-59791574 Msa0032620:intron 35.0%
TACCTAGATAAGCAATCTCT+AGG - chr1_1:59791560-59791579 None:intergenic 35.0%
TAGAGATTGCTTATCTAGGT+AGG + chr1_1:59791559-59791578 Msa0032620:intron 35.0%
CAACAAACTAAACCAACTGA+TGG - chr1_1:59791662-59791681 None:intergenic 35.0%
!!! AGTGTAATTTTTTCTGCTGC+TGG + chr1_1:59791733-59791752 Msa0032620:intron 35.0%
! AAAATGGATGATGGTTTGTG+GGG + chr1_1:59791865-59791884 Msa0032620:exon 35.0%
GATACTTTACAAGTGGATGA+CGG - chr1_1:59791955-59791974 None:intergenic 35.0%
AGATATATGGGAGAAGTTTG+TGG + chr1_1:59791988-59792007 Msa0032620:CDS 35.0%
AGGAAAAAAGTCAAAAGGGT+AGG + chr1_1:59792122-59792141 Msa0032620:intron 35.0%
GGAAAAAAGTCAAAAGGGTA+GGG + chr1_1:59792123-59792142 Msa0032620:intron 35.0%
GAGATAATGTATGTGGGTAA+AGG - chr1_1:59792165-59792184 None:intergenic 35.0%
AGAAAAAGAATGGAAGACTC+CGG + chr1_1:59792229-59792248 Msa0032620:CDS 35.0%
! ATATGTGTGTTTTGCAAACG+TGG + chr1_1:59792461-59792480 Msa0032620:three_prime_UTR 35.0%
! ATGAACCTTCTTGTTTGTTG+TGG + chr1_1:59792590-59792609 Msa0032620:three_prime_UTR 35.0%
TACATCCACAACAAACAAGA+AGG - chr1_1:59792598-59792617 None:intergenic 35.0%
ACAGCAACCAAAAACAAGAA+GGG - chr1_1:59792621-59792640 None:intergenic 35.0%
AACAGCAACCAAAAACAAGA+AGG - chr1_1:59792622-59792641 None:intergenic 35.0%
!! TGGGTTTCTGGAATGATTTG+CGG - chr1_1:59789205-59789224 None:intergenic 40.0%
GATGAGATTCAACCCCAAAT+CGG + chr1_1:59789270-59789289 Msa0032620:five_prime_UTR 40.0%
TGAGGTTGGATTTCCGATTT+GGG - chr1_1:59789286-59789305 None:intergenic 40.0%
TTGAGGTTGGATTTCCGATT+TGG - chr1_1:59789287-59789306 None:intergenic 40.0%
! CAATGATTTGCGGAGAAACA+CGG - chr1_1:59789350-59789369 None:intergenic 40.0%
GAGTCACGAACAATGATTTG+CGG - chr1_1:59789360-59789379 None:intergenic 40.0%
AAAGGTTCGTGACTCTCAAA+TGG + chr1_1:59789397-59789416 Msa0032620:intron 40.0%
AAGGTTCGTGACTCTCAAAT+GGG + chr1_1:59789398-59789417 Msa0032620:intron 40.0%
! GCTCTTTTCTCAAAAGGGTT+TGG + chr1_1:59789449-59789468 Msa0032620:intron 40.0%
GCTGCTACACAATCATCATA+AGG + chr1_1:59790041-59790060 Msa0032620:intron 40.0%
GCAGGTATGACATTCAAACA+GGG - chr1_1:59790076-59790095 None:intergenic 40.0%
TGCAGGTATGACATTCAAAC+AGG - chr1_1:59790077-59790096 None:intergenic 40.0%
CAATGACCCACAAGAATTCT+AGG + chr1_1:59790400-59790419 Msa0032620:intron 40.0%
ATTTGGACAATTCGGTCAAG+AGG - chr1_1:59790433-59790452 None:intergenic 40.0%
CTTGACCGAATTGTCCAAAT+AGG + chr1_1:59790433-59790452 Msa0032620:intron 40.0%
AGAATCAGGATGTGTATAGC+AGG - chr1_1:59790625-59790644 None:intergenic 40.0%
TTCACCTTTATCCCACGTAA+TGG + chr1_1:59790651-59790670 Msa0032620:intron 40.0%
TATAGAGAGAGCCATTACGT+GGG - chr1_1:59790665-59790684 None:intergenic 40.0%
ATATAGAGAGAGCCATTACG+TGG - chr1_1:59790666-59790685 None:intergenic 40.0%
!!! TCTTGTGGGGCTGTTTTTTT+GGG + chr1_1:59790900-59790919 Msa0032620:intron 40.0%
!! CTCGCTTTTGTTTGGGATAT+TGG + chr1_1:59791216-59791235 Msa0032620:intron 40.0%
! GCTTATGGTCTCTGTTTTGT+AGG + chr1_1:59791293-59791312 Msa0032620:intron 40.0%
!! TGAAGTTGGTGGAGTCAATA+AGG + chr1_1:59791433-59791452 Msa0032620:intron 40.0%
TTGCTTATCTAGGTAGGTGT+TGG + chr1_1:59791565-59791584 Msa0032620:intron 40.0%
!!! CTAGGTAGGTGTTGGTTTTT+TGG + chr1_1:59791573-59791592 Msa0032620:intron 40.0%
GTACAAATAGCCCAACTGTA+CGG + chr1_1:59791623-59791642 Msa0032620:intron 40.0%
AAACAAACTGCCGTACAGTT+GGG - chr1_1:59791636-59791655 None:intergenic 40.0%
! AGTTTGTTTGAGCCATCAGT+TGG + chr1_1:59791647-59791666 Msa0032620:intron 40.0%
AAAATGTCCCTAAAGCCACT+CGG - chr1_1:59791759-59791778 None:intergenic 40.0%
TCGAAAGACCCCTGAATTTA+AGG + chr1_1:59792015-59792034 Msa0032620:CDS 40.0%
TGTATGTGGGTAAAGGTTGT+TGG - chr1_1:59792158-59792177 None:intergenic 40.0%
TACCCACATACATTATCTCG+TGG + chr1_1:59792166-59792185 Msa0032620:CDS 40.0%
CTCCACGAGATAATGTATGT+GGG - chr1_1:59792171-59792190 None:intergenic 40.0%
TTCCATTTGTCATCACGTGA+CGG - chr1_1:59792280-59792299 None:intergenic 40.0%
! AAGAGAGCACGACAAAAGAA+AGG + chr1_1:59792298-59792317 Msa0032620:CDS 40.0%
! AGAGAGCACGACAAAAGAAA+GGG + chr1_1:59792299-59792318 Msa0032620:CDS 40.0%
! ACGTGGTACTGAAGATATGT+TGG + chr1_1:59792478-59792497 Msa0032620:three_prime_UTR 40.0%
!!! GGATGTACCCTTCTTGTTTT+TGG + chr1_1:59792611-59792630 Msa0032620:three_prime_UTR 40.0%
! AGCTTTGTGGTGAGAGTGAA+CGG - chr1_1:59789174-59789193 None:intergenic 45.0%
! TCTGGAATGATTTGCGGATC+TGG - chr1_1:59789199-59789218 None:intergenic 45.0%
TGGGGTTGAATCTCATCTCA+TGG - chr1_1:59789267-59789286 None:intergenic 45.0%
! GAGGTTGGATTTCCGATTTG+GGG - chr1_1:59789285-59789304 None:intergenic 45.0%
! AACGAGAGGAGATTTGAGGT+TGG - chr1_1:59789300-59789319 None:intergenic 45.0%
GAGTAACAATCACGAACGAG+AGG - chr1_1:59789314-59789333 None:intergenic 45.0%
TCTCAAGGTCTGACTACTAC+TGG - chr1_1:59789879-59789898 None:intergenic 45.0%
!! TTTTGGGGATCAGATGCCAA+AGG + chr1_1:59789967-59789986 Msa0032620:five_prime_UTR 45.0%
TTTGGGGATCAGATGCCAAA+GGG + chr1_1:59789968-59789987 Msa0032620:five_prime_UTR 45.0%
TGAACTCTGACACTCATCCT+TGG + chr1_1:59790485-59790504 Msa0032620:intron 45.0%
AAATGAATATGTCCTCGGCG+AGG + chr1_1:59790523-59790542 Msa0032620:intron 45.0%
AATTACTAAGATCCTCGCCG+AGG - chr1_1:59790538-59790557 None:intergenic 45.0%
AAGGTGAAGTCTCGAGAATC+AGG - chr1_1:59790639-59790658 None:intergenic 45.0%
AGAGCCATTACGTGGGATAA+AGG - chr1_1:59790658-59790677 None:intergenic 45.0%
TGGAACCCAAGAGATTGTCT+TGG + chr1_1:59790848-59790867 Msa0032620:intron 45.0%
!!! AATGGGGTTTTTGGCTCTTG+TGG + chr1_1:59790885-59790904 Msa0032620:intron 45.0%
!!! ATGGGGTTTTTGGCTCTTGT+GGG + chr1_1:59790886-59790905 Msa0032620:intron 45.0%
!!! CTCTTGTGGGGCTGTTTTTT+TGG + chr1_1:59790899-59790918 Msa0032620:intron 45.0%
!!! GGGCTGTTTTTTTGGGACTA+TGG + chr1_1:59790907-59790926 Msa0032620:intron 45.0%
AAAAAATGACCTGTCCAGGG+AGG - chr1_1:59791003-59791022 None:intergenic 45.0%
!! AGTTGGTGGAGTCAATAAGG+AGG + chr1_1:59791436-59791455 Msa0032620:intron 45.0%
!!! GGTTTTGTTTGACGCGGTAA+AGG + chr1_1:59791463-59791482 Msa0032620:intron 45.0%
! TAAAGGCCTTATCTTGGTGC+CGG + chr1_1:59791480-59791499 Msa0032620:intron 45.0%
CAAACAAACTGCCGTACAGT+TGG - chr1_1:59791637-59791656 None:intergenic 45.0%
AGAGACATAGAAACAGCAGC+AGG - chr1_1:59791692-59791711 None:intergenic 45.0%
GGATCTGGATCATCACTTTC+TGG - chr1_1:59791824-59791843 None:intergenic 45.0%
GTGTCTTAAAACCTCTCCAC+CGG - chr1_1:59791892-59791911 None:intergenic 45.0%
CCTCCACGAGATAATGTATG+TGG - chr1_1:59792172-59792191 None:intergenic 45.0%
CCACATACATTATCTCGTGG+AGG + chr1_1:59792169-59792188 Msa0032620:CDS 45.0%
! GAGAGCACGACAAAAGAAAG+GGG + chr1_1:59792300-59792319 Msa0032620:CDS 45.0%
AGAGCACGACAAAAGAAAGG+GGG + chr1_1:59792301-59792320 Msa0032620:CDS 45.0%
!! TTAACTATATATTAATTAAT+TGG + chr1_1:59789783-59789802 Msa0032620:intron 5.0%
TTTGTGGTGAGAGGGGTAGA+GGG - chr1_1:59789117-59789136 None:intergenic 50.0%
! TGGAGAGCTTTGTGGTGAGA+GGG - chr1_1:59789125-59789144 None:intergenic 50.0%
ACTGGATCTGGAGAGCTTTG+TGG - chr1_1:59789133-59789152 None:intergenic 50.0%
TGGTGAGAGTGAACTGGATC+TGG - chr1_1:59789145-59789164 None:intergenic 50.0%
CGAATCTGGTGAGAGTGAAC+TGG - chr1_1:59789151-59789170 None:intergenic 50.0%
CACGAACGAGAGGAGATTTG+AGG - chr1_1:59789304-59789323 None:intergenic 50.0%
! TGACTACTACTGGTGGTGCA+TGG - chr1_1:59789869-59789888 None:intergenic 50.0%
CAAGGTCTGACTACTACTGG+TGG - chr1_1:59789876-59789895 None:intergenic 50.0%
TGCTTCATGGAGGCAAAGTG+AGG - chr1_1:59789935-59789954 None:intergenic 50.0%
TGGACAATTCGGTCAAGAGG+TGG - chr1_1:59790430-59790449 None:intergenic 50.0%
!!! TGGGGTTTTTGGCTCTTGTG+GGG + chr1_1:59790887-59790906 Msa0032620:intron 50.0%
TGGTGGAGTCAATAAGGAGG+CGG + chr1_1:59791439-59791458 Msa0032620:intron 50.0%
! ACGCGGTAAAGGCCTTATCT+TGG + chr1_1:59791474-59791493 Msa0032620:intron 50.0%
AAGACACCGGCACCAAGATA+AGG - chr1_1:59791489-59791508 None:intergenic 50.0%
! GATGACGGCAGTCTTTGGAA+GGG - chr1_1:59791940-59791959 None:intergenic 50.0%
! AAGTGGATGACGGCAGTCTT+TGG - chr1_1:59791945-59791964 None:intergenic 50.0%
CATTTGTCATCACGTGACGG+CGG - chr1_1:59792277-59792296 None:intergenic 50.0%
AGGGGGAGAATACACAACAG+AGG + chr1_1:59792318-59792337 Msa0032620:CDS 50.0%
TGAGAGGGGTAGAGGGTTTG+TGG - chr1_1:59789110-59789129 None:intergenic 55.0%
CTTTGTGGTGAGAGGGGTAG+AGG - chr1_1:59789118-59789137 None:intergenic 55.0%
! GGAGAGCTTTGTGGTGAGAG+GGG - chr1_1:59789124-59789143 None:intergenic 55.0%
! CTGGAGAGCTTTGTGGTGAG+AGG - chr1_1:59789126-59789145 None:intergenic 55.0%
GTGAGAGTGAACGGCGAATC+TGG - chr1_1:59789165-59789184 None:intergenic 55.0%
TGCGGATCTGGAGAGCTTTG+TGG - chr1_1:59789187-59789206 None:intergenic 55.0%
!! GTGAGCTTGCTGAGCTTGCA+AGG + chr1_1:59790365-59790384 Msa0032620:intron 55.0%
TGGAGTCAATAAGGAGGCGG+AGG + chr1_1:59791442-59791461 Msa0032620:intron 55.0%
!!! GGCGGAGGTTTTGTTTGACG+CGG + chr1_1:59791457-59791476 Msa0032620:intron 55.0%
!!! GGTTTTTTGGTGCAGCTGCC+CGG + chr1_1:59791586-59791605 Msa0032620:intron 55.0%
! TTTTTCTGCTGCTGGCCGAG+TGG + chr1_1:59791741-59791760 Msa0032620:intron 55.0%
! GGATGATGGTTTGTGGGGAC+CGG + chr1_1:59791870-59791889 Msa0032620:exon 55.0%
! GGATGACGGCAGTCTTTGGA+AGG - chr1_1:59791941-59791960 None:intergenic 55.0%
CGCCGTCACGTGATGACAAA+TGG + chr1_1:59792275-59792294 Msa0032620:CDS 55.0%
GTCATTGTGCCTCCCTGGAC+AGG + chr1_1:59790991-59791010 Msa0032620:intron 60.0%
TGTACGCCGCACACCAAAGC+CGG - chr1_1:59791608-59791627 None:intergenic 60.0%
! CTGCTGGCCGAGTGGCTTTA+GGG + chr1_1:59791749-59791768 Msa0032620:intron 60.0%
! TGATGGTTTGTGGGGACCGG+TGG + chr1_1:59791873-59791892 Msa0032620:CDS 60.0%
! GGCGTGTCATTGTGCCTCCC+TGG + chr1_1:59790986-59791005 Msa0032620:intron 65.0%
TTGGTGCAGCTGCCCGGCTT+TGG + chr1_1:59791592-59791611 Msa0032620:intron 65.0%
! AGCTGCCCGGCTTTGGTGTG+CGG + chr1_1:59791599-59791618 Msa0032620:intron 65.0%
GTACGCCGCACACCAAAGCC+GGG - chr1_1:59791607-59791626 None:intergenic 65.0%
GCTGCTGGCCGAGTGGCTTT+AGG + chr1_1:59791748-59791767 Msa0032620:intron 65.0%
GTTTGTGGGGACCGGTGGAG+AGG + chr1_1:59791878-59791897 Msa0032620:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 59789101 59792729 59789101 ID=Msa0032620;Name=Msa0032620
chr1_1 mRNA 59789101 59792729 59789101 ID=Msa0032620-mRNA-1;Parent=Msa0032620;Name=Msa0032620-mRNA-1;_AED=0.15;_eAED=0.15;_QI=594|1|1|1|0.25|0.6|5|333|145
chr1_1 exon 59789101 59789400 59789101 ID=Msa0032620-mRNA-1:exon:18568;Parent=Msa0032620-mRNA-1
chr1_1 exon 59789840 59789989 59789840 ID=Msa0032620-mRNA-1:exon:18569;Parent=Msa0032620-mRNA-1
chr1_1 exon 59791121 59791198 59791121 ID=Msa0032620-mRNA-1:exon:18570;Parent=Msa0032620-mRNA-1
chr1_1 exon 59791806 59792036 59791806 ID=Msa0032620-mRNA-1:exon:18571;Parent=Msa0032620-mRNA-1
chr1_1 exon 59792124 59792729 59792124 ID=Msa0032620-mRNA-1:exon:18572;Parent=Msa0032620-mRNA-1
chr1_1 five_prime_UTR 59789101 59789400 59789101 ID=Msa0032620-mRNA-1:five_prime_utr;Parent=Msa0032620-mRNA-1
chr1_1 five_prime_UTR 59789840 59789989 59789840 ID=Msa0032620-mRNA-1:five_prime_utr;Parent=Msa0032620-mRNA-1
chr1_1 five_prime_UTR 59791121 59791198 59791121 ID=Msa0032620-mRNA-1:five_prime_utr;Parent=Msa0032620-mRNA-1
chr1_1 five_prime_UTR 59791806 59791871 59791806 ID=Msa0032620-mRNA-1:five_prime_utr;Parent=Msa0032620-mRNA-1
chr1_1 CDS 59791872 59792036 59791872 ID=Msa0032620-mRNA-1:cds;Parent=Msa0032620-mRNA-1
chr1_1 CDS 59792124 59792396 59792124 ID=Msa0032620-mRNA-1:cds;Parent=Msa0032620-mRNA-1
chr1_1 three_prime_UTR 59792397 59792729 59792397 ID=Msa0032620-mRNA-1:three_prime_utr;Parent=Msa0032620-mRNA-1
Gene Sequence

>Msa0032620

ATGATGGTTTGTGGGGACCGGTGGAGAGGTTTTAAGACACAACTCACAAATGATTATATTACAAACCCTTCCAAAGACTGCCGTCATCCACTTGTAAAGTATCCTTTTCTTCAAAAAGATATATGGGAGAAGTTTGTGGAGTCTCGAAAGACCCCTGAATTTAAGGAAAAAAGTCAAAAGGGTAGGGATACTGTTGCCAACAACCTTTACCCACATACATTATCTCGTGGAGGATATGATTACCTTGAAGATAAAATGATGGATGAAAAGAGAAAAAGAATGGAAGACTCCGGAGAATTTGATCATATTCTATCTCCGCCGTCACGTGATGACAAATGGAAGAGAGCACGACAAAAGAAAGGGGGAGAATACACAACAGAGGCTACACGAGTTGTTGCTGAGAAAATTGTAAGTAATATGAGTAATGAGTCTTATTAA
Protein sequence

>Msa0032620

MMVCGDRWRGFKTQLTNDYITNPSKDCRHPLVKYPFLQKDIWEKFVESRKTPEFKEKSQKGRDTVANNLYPHTLSRGGYDYLEDKMMDEKRKRMEDSGEFDHILSPPSRDDKWKRARQKKGGEYTTEATRVVAEKIVSNMSNESY*