AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1142990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATTTCATATAGATTTA+AGG 0.126167 7_4:+18192557 Msa1142990:CDS
CACATTACTTCCCTTGTTTC+AGG 0.156799 7_4:-18191882 None:intergenic
TTTCTTGGTATGATGTTATA+TGG 0.184558 7_4:+18192207 Msa1142990:exon
TCAAATCTTGAAATTTATAT+TGG 0.246513 7_4:+18192281 Msa1142990:CDS
CGTGTATTCTCTATACTAAT+TGG 0.265886 7_4:-18191185 None:intergenic
ACCACCATTGGTTGGCAATA+TGG 0.275006 7_4:-18192791 None:intergenic
AAAGATGAAGACTGAACTAT+TGG 0.311734 7_4:-18191233 None:intergenic
CTATATGAAATCAAAGTTAT+AGG 0.321947 7_4:-18192549 None:intergenic
AAACTGCTTCACTTCACTAA+TGG 0.340507 7_4:+18191918 Msa1142990:five_prime_UTR
GAAACGGAAGAAGAATTTCT+TGG 0.371083 7_4:+18192192 Msa1142990:five_prime_UTR
ATTTATATTGGTTGAAATGA+GGG 0.398294 7_4:+18192293 Msa1142990:CDS
GCATAGCATAGGAGCTTCAT+TGG 0.413800 7_4:+18192320 Msa1142990:CDS
CACCACACAATTGACCAATC+AGG 0.421193 7_4:+18191143 Msa1142990:five_prime_UTR
TTCTTGGTATGATGTTATAT+GGG 0.444798 7_4:+18192208 Msa1142990:exon
AGAAACACAACCATTCCTTC+TGG 0.446249 7_4:+18191318 Msa1142990:five_prime_UTR
ATCATCGTCGGGACCGGCAT+CGG 0.456038 7_4:+18191431 Msa1142990:five_prime_UTR
GGATGAAGACGGCGAGGATG+TGG 0.464192 7_4:+18191953 Msa1142990:five_prime_UTR
TTTATCTCCATCGGAGTTGA+CGG 0.464855 7_4:-18191510 None:intergenic
ATGAGGAGGGAGGTGGTCGC+CGG 0.466074 7_4:-18191364 None:intergenic
GTGTATTCTCTATACTAATT+GGG 0.470158 7_4:-18191184 None:intergenic
TCTTCTTTCATCACCGCCGC+TGG 0.484557 7_4:+18191485 Msa1142990:five_prime_UTR
TCGGGACCGGCATCGGAGTT+AGG 0.488644 7_4:+18191438 Msa1142990:five_prime_UTR
ACCGTCATCATCGTCGGGAC+CGG 0.492085 7_4:+18191425 Msa1142990:five_prime_UTR
AATTTATATTGGTTGAAATG+AGG 0.493939 7_4:+18192292 Msa1142990:CDS
AGGAGGGAGGTGGTCGCCGG+TGG 0.496894 7_4:-18191361 None:intergenic
GACGATGATGACGGTGGCGG+TGG 0.499655 7_4:-18191417 None:intergenic
TGGAGATAAATTAATGTTGT+CGG 0.510571 7_4:+18191523 Msa1142990:five_prime_UTR
AAGATGAAGACTGAACTATT+GGG 0.518628 7_4:-18191232 None:intergenic
GAGGATATCTCAAACCTGAT+TGG 0.519181 7_4:-18191157 None:intergenic
CAACATTAATTTATCTCCAT+CGG 0.523467 7_4:-18191519 None:intergenic
ACCGCCACCGTCATCATCGT+CGG 0.526521 7_4:+18191419 Msa1142990:five_prime_UTR
GGTTGAAGAATATGAAGAAA+CGG 0.528087 7_4:+18192176 Msa1142990:five_prime_UTR
CCGCCACCGTCATCATCGTC+GGG 0.534818 7_4:+18191420 Msa1142990:five_prime_UTR
CAGTGAATGTTCCGCCGACA+TGG 0.536269 7_4:+18191715 Msa1142990:five_prime_UTR
TGTACTTTAATCAGCGGCGA+TGG 0.537118 7_4:-18191566 None:intergenic
GCTGGATCCGTCAACTCCGA+TGG 0.548407 7_4:+18191503 Msa1142990:five_prime_UTR
GATCAACGAACCAGAAGGAA+TGG 0.553626 7_4:-18191328 None:intergenic
GCCATATTGCCAACCAATGG+TGG 0.555458 7_4:+18192790 Msa1142990:CDS
CAACAGCCTAACTCCGATGC+CGG 0.564596 7_4:-18191444 None:intergenic
GTAGCCATATTGCCAACCAA+TGG 0.566165 7_4:+18192787 Msa1142990:CDS
TCGGCGGACATCAGAGAAGA+AGG 0.568449 7_4:+18191679 Msa1142990:five_prime_UTR
CATACAAATGAGGAGGGAGG+TGG 0.570234 7_4:-18191371 None:intergenic
CCCGACGATGATGACGGTGG+CGG 0.576884 7_4:-18191420 None:intergenic
ACGAAATTCGTCGACGACGG+CGG 0.581665 7_4:+18191630 Msa1142990:five_prime_UTR
TTTACGAAATTCGTCGACGA+CGG 0.585253 7_4:+18191627 Msa1142990:five_prime_UTR
TTTGATTGTACTTTAATCAG+CGG 0.589145 7_4:-18191572 None:intergenic
AGAAAACAGTTGATGATGGT+CGG 0.590020 7_4:+18191660 Msa1142990:five_prime_UTR
AAATGACTGTCATACAAATG+AGG 0.592160 7_4:-18191381 None:intergenic
ATTGAGCAACTCCTGAAACA+AGG 0.592296 7_4:+18191871 Msa1142990:five_prime_UTR
TTGAGCAACTCCTGAAACAA+GGG 0.592522 7_4:+18191872 Msa1142990:five_prime_UTR
TCGTTCAAACCACCATTGGT+TGG 0.594280 7_4:-18192799 None:intergenic
GACTGTCATACAAATGAGGA+GGG 0.598788 7_4:-18191377 None:intergenic
GTATAGAAAACAGTTGATGA+TGG 0.601792 7_4:+18191656 Msa1142990:five_prime_UTR
TCTATACTAATTGGGAGGAG+AGG 0.605679 7_4:-18191176 None:intergenic
TTGTTTAAATTAGGACTCTG+AGG 0.606909 7_4:+18192155 Msa1142990:intron
CACTAATGGAGATGAAGTAG+AGG 0.611633 7_4:+18191932 Msa1142990:five_prime_UTR
TGACTGTCATACAAATGAGG+AGG 0.612985 7_4:-18191378 None:intergenic
GCCGGTCCCGACGATGATGA+CGG 0.616076 7_4:-18191426 None:intergenic
ATGAGGGTAAAGCATAGCAT+AGG 0.621957 7_4:+18192309 Msa1142990:CDS
AGTTATAGGATATGAATGCA+CGG 0.631860 7_4:-18192535 None:intergenic
CTGAAACAAGGGAAGTAATG+TGG 0.631916 7_4:+18191883 Msa1142990:five_prime_UTR
ATCGGAGTTGACGGATCCAG+CGG 0.632959 7_4:-18191501 None:intergenic
GATGAAGTAGAGGATGAAGA+CGG 0.655937 7_4:+18191942 Msa1142990:five_prime_UTR
CTGATTGGTCAATTGTGTGG+TGG 0.657391 7_4:-18191142 None:intergenic
ATGAAGACTGAACTATTGGG+AGG 0.658700 7_4:-18191229 None:intergenic
TATTCTCTATACTAATTGGG+AGG 0.664675 7_4:-18191181 None:intergenic
AACCTGATTGGTCAATTGTG+TGG 0.669078 7_4:-18191145 None:intergenic
GGAGTTGACGGATCCAGCGG+CGG 0.672445 7_4:-18191498 None:intergenic
AAACAGTTGATGATGGTCGG+CGG 0.701472 7_4:+18191663 Msa1142990:five_prime_UTR
AGTAGAGGATGAAGACGGCG+AGG 0.703240 7_4:+18191947 Msa1142990:five_prime_UTR
TGTCATACAAATGAGGAGGG+AGG 0.710866 7_4:-18191374 None:intergenic
GGTCCCGACGATGATGACGG+TGG 0.722934 7_4:-18191423 None:intergenic
TGACTCTCAAGATTACGATG+AGG 0.745857 7_4:+18191989 Msa1142990:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTATTTCAAAAAAT+AGG - chr7_4:18191285-18191304 None:intergenic 10.0%
!!! ATGACTTTAATTTATTTATT+GGG + chr7_4:18191818-18191837 Msa1142990:intron 10.0%
!! AAATTCAAAACATAAAAAAT+GGG - chr7_4:18192619-18192638 None:intergenic 10.0%
!! AAAATTCAAAACATAAAAAA+TGG - chr7_4:18192620-18192639 None:intergenic 10.0%
!!! CATTTTTTATTTCAAAAAAT+AGG - chr7_4:18191285-18191304 None:intergenic 10.0%
!!! ATGACTTTAATTTATTTATT+GGG + chr7_4:18191818-18191837 Msa1142990:intron 10.0%
!! AAATTCAAAACATAAAAAAT+GGG - chr7_4:18192619-18192638 None:intergenic 10.0%
!! AAAATTCAAAACATAAAAAA+TGG - chr7_4:18192620-18192639 None:intergenic 10.0%
!!! TTTTTATTTCAAAAAATAGG+AGG - chr7_4:18191282-18191301 None:intergenic 15.0%
!!! ATTTTCTGTTTTTTTCATAT+GGG - chr7_4:18191599-18191618 None:intergenic 15.0%
!!! TATTTTCTGTTTTTTTCATA+TGG - chr7_4:18191600-18191619 None:intergenic 15.0%
!! CAATAAATAAATTAAAGTCA+TGG - chr7_4:18191819-18191838 None:intergenic 15.0%
!!! CATGACTTTAATTTATTTAT+TGG + chr7_4:18191817-18191836 Msa1142990:intron 15.0%
!! TCAAATCTTGAAATTTATAT+TGG + chr7_4:18192281-18192300 Msa1142990:CDS 15.0%
!!! TTTGATTTCATATAGATTTA+AGG + chr7_4:18192557-18192576 Msa1142990:CDS 15.0%
!! AATTCAAAACATAAAAAATG+GGG - chr7_4:18192618-18192637 None:intergenic 15.0%
!!! TTTTTATTTCAAAAAATAGG+AGG - chr7_4:18191282-18191301 None:intergenic 15.0%
!!! ATTTTCTGTTTTTTTCATAT+GGG - chr7_4:18191599-18191618 None:intergenic 15.0%
!!! TATTTTCTGTTTTTTTCATA+TGG - chr7_4:18191600-18191619 None:intergenic 15.0%
!! CAATAAATAAATTAAAGTCA+TGG - chr7_4:18191819-18191838 None:intergenic 15.0%
!!! CATGACTTTAATTTATTTAT+TGG + chr7_4:18191817-18191836 Msa1142990:intron 15.0%
!! TCAAATCTTGAAATTTATAT+TGG + chr7_4:18192281-18192300 Msa1142990:CDS 15.0%
!!! TTTGATTTCATATAGATTTA+AGG + chr7_4:18192557-18192576 Msa1142990:CDS 15.0%
!! AATTCAAAACATAAAAAATG+GGG - chr7_4:18192618-18192637 None:intergenic 15.0%
!!! AATTTATATTGGTTGAAATG+AGG + chr7_4:18192292-18192311 Msa1142990:CDS 20.0%
!!! ATTTATATTGGTTGAAATGA+GGG + chr7_4:18192293-18192312 Msa1142990:CDS 20.0%
!! CTATATGAAATCAAAGTTAT+AGG - chr7_4:18192552-18192571 None:intergenic 20.0%
!!! TTTATTATTGTTTTCAACAG+TGG + chr7_4:18192638-18192657 Msa1142990:intron 20.0%
!!! AATTTATATTGGTTGAAATG+AGG + chr7_4:18192292-18192311 Msa1142990:CDS 20.0%
!!! ATTTATATTGGTTGAAATGA+GGG + chr7_4:18192293-18192312 Msa1142990:CDS 20.0%
!! CTATATGAAATCAAAGTTAT+AGG - chr7_4:18192552-18192571 None:intergenic 20.0%
!!! TTTATTATTGTTTTCAACAG+TGG + chr7_4:18192638-18192657 Msa1142990:intron 20.0%
! GTGTATTCTCTATACTAATT+GGG - chr7_4:18191187-18191206 None:intergenic 25.0%
! CAACATTAATTTATCTCCAT+CGG - chr7_4:18191522-18191541 None:intergenic 25.0%
! TGGAGATAAATTAATGTTGT+CGG + chr7_4:18191523-18191542 Msa1142990:five_prime_UTR 25.0%
! TTTGATTGTACTTTAATCAG+CGG - chr7_4:18191575-18191594 None:intergenic 25.0%
! TTTAACAGTTCTTAATCTTG+TGG + chr7_4:18192130-18192149 Msa1142990:intron 25.0%
! TTAACAGTTCTTAATCTTGT+GGG + chr7_4:18192131-18192150 Msa1142990:intron 25.0%
! TTTCTTGGTATGATGTTATA+TGG + chr7_4:18192207-18192226 Msa1142990:exon 25.0%
! TTCTTGGTATGATGTTATAT+GGG + chr7_4:18192208-18192227 Msa1142990:exon 25.0%
!!! TTTTGAAACTATTTGAGTTG+TGG + chr7_4:18192235-18192254 Msa1142990:CDS 25.0%
!!! TGATGATTTTGAAATTGACT+TGG + chr7_4:18192705-18192724 Msa1142990:CDS 25.0%
!!! AGTGTGATTATTTTGAGATT+GGG + chr7_4:18192740-18192759 Msa1142990:CDS 25.0%
! GTGTATTCTCTATACTAATT+GGG - chr7_4:18191187-18191206 None:intergenic 25.0%
! CAACATTAATTTATCTCCAT+CGG - chr7_4:18191522-18191541 None:intergenic 25.0%
! TGGAGATAAATTAATGTTGT+CGG + chr7_4:18191523-18191542 Msa1142990:five_prime_UTR 25.0%
! TTTGATTGTACTTTAATCAG+CGG - chr7_4:18191575-18191594 None:intergenic 25.0%
! TTTAACAGTTCTTAATCTTG+TGG + chr7_4:18192130-18192149 Msa1142990:intron 25.0%
! TTAACAGTTCTTAATCTTGT+GGG + chr7_4:18192131-18192150 Msa1142990:intron 25.0%
! TTTCTTGGTATGATGTTATA+TGG + chr7_4:18192207-18192226 Msa1142990:exon 25.0%
! TTCTTGGTATGATGTTATAT+GGG + chr7_4:18192208-18192227 Msa1142990:exon 25.0%
!!! TTTTGAAACTATTTGAGTTG+TGG + chr7_4:18192235-18192254 Msa1142990:CDS 25.0%
!!! TGATGATTTTGAAATTGACT+TGG + chr7_4:18192705-18192724 Msa1142990:CDS 25.0%
!!! AGTGTGATTATTTTGAGATT+GGG + chr7_4:18192740-18192759 Msa1142990:CDS 25.0%
TATTCTCTATACTAATTGGG+AGG - chr7_4:18191184-18191203 None:intergenic 30.0%
CGTGTATTCTCTATACTAAT+TGG - chr7_4:18191188-18191207 None:intergenic 30.0%
AAGATGAAGACTGAACTATT+GGG - chr7_4:18191235-18191254 None:intergenic 30.0%
AAAGATGAAGACTGAACTAT+TGG - chr7_4:18191236-18191255 None:intergenic 30.0%
TTCAAAAAATAGGAGGAGAT+GGG - chr7_4:18191275-18191294 None:intergenic 30.0%
TTTCAAAAAATAGGAGGAGA+TGG - chr7_4:18191276-18191295 None:intergenic 30.0%
AAATGACTGTCATACAAATG+AGG - chr7_4:18191384-18191403 None:intergenic 30.0%
!! GTATAGAAAACAGTTGATGA+TGG + chr7_4:18191656-18191675 Msa1142990:five_prime_UTR 30.0%
GTGAAAAAATTGAACCATGT+CGG - chr7_4:18191732-18191751 None:intergenic 30.0%
!!! ACATGGTTCAATTTTTTCAC+AGG + chr7_4:18191732-18191751 Msa1142990:five_prime_UTR 30.0%
!! TATTGGGTGAATTTTAGTGA+TGG + chr7_4:18191834-18191853 Msa1142990:intron 30.0%
! TATATCTCCTTTTGTCTACA+AGG + chr7_4:18192064-18192083 Msa1142990:intron 30.0%
! CTTGTGGGTTTGTTTAAATT+AGG + chr7_4:18192146-18192165 Msa1142990:intron 30.0%
TTGTTTAAATTAGGACTCTG+AGG + chr7_4:18192155-18192174 Msa1142990:intron 30.0%
GGTTGAAGAATATGAAGAAA+CGG + chr7_4:18192176-18192195 Msa1142990:five_prime_UTR 30.0%
AAATGTTATACTTGAGAGTG+AGG - chr7_4:18192415-18192434 None:intergenic 30.0%
TGCACAAAATAGTGAATACA+TGG - chr7_4:18192445-18192464 None:intergenic 30.0%
! AATTATGTCCCATTTTCTTC+TGG + chr7_4:18192492-18192511 Msa1142990:intron 30.0%
CTGAAAAACCAGAAGAAAAT+GGG - chr7_4:18192503-18192522 None:intergenic 30.0%
AGTTATAGGATATGAATGCA+CGG - chr7_4:18192538-18192557 None:intergenic 30.0%
AGTGGTAATAACAACTAGAT+AGG + chr7_4:18192656-18192675 Msa1142990:intron 30.0%
ACAACTAGATAGGATTAACT+TGG + chr7_4:18192666-18192685 Msa1142990:intron 30.0%
!!! GAGTGTGATTATTTTGAGAT+TGG + chr7_4:18192739-18192758 Msa1142990:CDS 30.0%
!!! GTGTGATTATTTTGAGATTG+GGG + chr7_4:18192741-18192760 Msa1142990:CDS 30.0%
TATTCTCTATACTAATTGGG+AGG - chr7_4:18191184-18191203 None:intergenic 30.0%
CGTGTATTCTCTATACTAAT+TGG - chr7_4:18191188-18191207 None:intergenic 30.0%
AAGATGAAGACTGAACTATT+GGG - chr7_4:18191235-18191254 None:intergenic 30.0%
AAAGATGAAGACTGAACTAT+TGG - chr7_4:18191236-18191255 None:intergenic 30.0%
TTCAAAAAATAGGAGGAGAT+GGG - chr7_4:18191275-18191294 None:intergenic 30.0%
TTTCAAAAAATAGGAGGAGA+TGG - chr7_4:18191276-18191295 None:intergenic 30.0%
AAATGACTGTCATACAAATG+AGG - chr7_4:18191384-18191403 None:intergenic 30.0%
!! GTATAGAAAACAGTTGATGA+TGG + chr7_4:18191656-18191675 Msa1142990:five_prime_UTR 30.0%
GTGAAAAAATTGAACCATGT+CGG - chr7_4:18191732-18191751 None:intergenic 30.0%
!!! ACATGGTTCAATTTTTTCAC+AGG + chr7_4:18191732-18191751 Msa1142990:five_prime_UTR 30.0%
!! TATTGGGTGAATTTTAGTGA+TGG + chr7_4:18191834-18191853 Msa1142990:intron 30.0%
! TATATCTCCTTTTGTCTACA+AGG + chr7_4:18192064-18192083 Msa1142990:intron 30.0%
! CTTGTGGGTTTGTTTAAATT+AGG + chr7_4:18192146-18192165 Msa1142990:intron 30.0%
TTGTTTAAATTAGGACTCTG+AGG + chr7_4:18192155-18192174 Msa1142990:intron 30.0%
GGTTGAAGAATATGAAGAAA+CGG + chr7_4:18192176-18192195 Msa1142990:five_prime_UTR 30.0%
AAATGTTATACTTGAGAGTG+AGG - chr7_4:18192415-18192434 None:intergenic 30.0%
TGCACAAAATAGTGAATACA+TGG - chr7_4:18192445-18192464 None:intergenic 30.0%
! AATTATGTCCCATTTTCTTC+TGG + chr7_4:18192492-18192511 Msa1142990:intron 30.0%
CTGAAAAACCAGAAGAAAAT+GGG - chr7_4:18192503-18192522 None:intergenic 30.0%
AGTTATAGGATATGAATGCA+CGG - chr7_4:18192538-18192557 None:intergenic 30.0%
AGTGGTAATAACAACTAGAT+AGG + chr7_4:18192656-18192675 Msa1142990:intron 30.0%
ACAACTAGATAGGATTAACT+TGG + chr7_4:18192666-18192685 Msa1142990:intron 30.0%
!!! GAGTGTGATTATTTTGAGAT+TGG + chr7_4:18192739-18192758 Msa1142990:CDS 30.0%
!!! GTGTGATTATTTTGAGATTG+GGG + chr7_4:18192741-18192760 Msa1142990:CDS 30.0%
AAAAAGATCAACGAACCAGA+AGG - chr7_4:18191336-18191355 None:intergenic 35.0%
!! TTGATCTTTTTCACTTCCAC+CGG + chr7_4:18191345-18191364 Msa1142990:five_prime_UTR 35.0%
!! AGAAAACAGTTGATGATGGT+CGG + chr7_4:18191660-18191679 Msa1142990:five_prime_UTR 35.0%
AAAAAATTGAACCATGTCGG+CGG - chr7_4:18191729-18191748 None:intergenic 35.0%
AAACTGCTTCACTTCACTAA+TGG + chr7_4:18191918-18191937 Msa1142990:five_prime_UTR 35.0%
GAAACGGAAGAAGAATTTCT+TGG + chr7_4:18192192-18192211 Msa1142990:five_prime_UTR 35.0%
!! TCATTGGATGTGATTTTGAC+AGG + chr7_4:18192336-18192355 Msa1142990:CDS 35.0%
TTTGACAGGTTTGAGTGTAT+TGG + chr7_4:18192350-18192369 Msa1142990:intron 35.0%
TTATACTTGAGAGTGAGGTT+TGG - chr7_4:18192410-18192429 None:intergenic 35.0%
TGGGACATAATTCAGTCAAA+TGG - chr7_4:18192484-18192503 None:intergenic 35.0%
CCTGAAAAACCAGAAGAAAA+TGG - chr7_4:18192504-18192523 None:intergenic 35.0%
!!! CCATTTTCTTCTGGTTTTTC+AGG + chr7_4:18192501-18192520 Msa1142990:intron 35.0%
! ATTTTCGTTCAAACCACCAT+TGG - chr7_4:18192806-18192825 None:intergenic 35.0%
AAAAAGATCAACGAACCAGA+AGG - chr7_4:18191336-18191355 None:intergenic 35.0%
!! TTGATCTTTTTCACTTCCAC+CGG + chr7_4:18191345-18191364 Msa1142990:five_prime_UTR 35.0%
!! AGAAAACAGTTGATGATGGT+CGG + chr7_4:18191660-18191679 Msa1142990:five_prime_UTR 35.0%
AAAAAATTGAACCATGTCGG+CGG - chr7_4:18191729-18191748 None:intergenic 35.0%
AAACTGCTTCACTTCACTAA+TGG + chr7_4:18191918-18191937 Msa1142990:five_prime_UTR 35.0%
GAAACGGAAGAAGAATTTCT+TGG + chr7_4:18192192-18192211 Msa1142990:five_prime_UTR 35.0%
!! TCATTGGATGTGATTTTGAC+AGG + chr7_4:18192336-18192355 Msa1142990:CDS 35.0%
TTTGACAGGTTTGAGTGTAT+TGG + chr7_4:18192350-18192369 Msa1142990:intron 35.0%
TTATACTTGAGAGTGAGGTT+TGG - chr7_4:18192410-18192429 None:intergenic 35.0%
TGGGACATAATTCAGTCAAA+TGG - chr7_4:18192484-18192503 None:intergenic 35.0%
CCTGAAAAACCAGAAGAAAA+TGG - chr7_4:18192504-18192523 None:intergenic 35.0%
!!! CCATTTTCTTCTGGTTTTTC+AGG + chr7_4:18192501-18192520 Msa1142990:intron 35.0%
! ATTTTCGTTCAAACCACCAT+TGG - chr7_4:18192806-18192825 None:intergenic 35.0%
! AACCTGATTGGTCAATTGTG+TGG - chr7_4:18191148-18191167 None:intergenic 40.0%
GAGGATATCTCAAACCTGAT+TGG - chr7_4:18191160-18191179 None:intergenic 40.0%
TCTATACTAATTGGGAGGAG+AGG - chr7_4:18191179-18191198 None:intergenic 40.0%
! TGGGAGGAGATGATTTTGTA+TGG - chr7_4:18191216-18191235 None:intergenic 40.0%
ATGAAGACTGAACTATTGGG+AGG - chr7_4:18191232-18191251 None:intergenic 40.0%
AGAAACACAACCATTCCTTC+TGG + chr7_4:18191318-18191337 Msa1142990:five_prime_UTR 40.0%
GACTGTCATACAAATGAGGA+GGG - chr7_4:18191380-18191399 None:intergenic 40.0%
TGACTGTCATACAAATGAGG+AGG - chr7_4:18191381-18191400 None:intergenic 40.0%
TTTATCTCCATCGGAGTTGA+CGG - chr7_4:18191513-18191532 None:intergenic 40.0%
TTTACGAAATTCGTCGACGA+CGG + chr7_4:18191627-18191646 Msa1142990:five_prime_UTR 40.0%
!! GTGAATTTTAGTGATGGCAC+TGG + chr7_4:18191840-18191859 Msa1142990:intron 40.0%
ATTGAGCAACTCCTGAAACA+AGG + chr7_4:18191871-18191890 Msa1142990:five_prime_UTR 40.0%
TTGAGCAACTCCTGAAACAA+GGG + chr7_4:18191872-18191891 Msa1142990:five_prime_UTR 40.0%
! CACATTACTTCCCTTGTTTC+AGG - chr7_4:18191885-18191904 None:intergenic 40.0%
CTGAAACAAGGGAAGTAATG+TGG + chr7_4:18191883-18191902 Msa1142990:five_prime_UTR 40.0%
CACTAATGGAGATGAAGTAG+AGG + chr7_4:18191932-18191951 Msa1142990:five_prime_UTR 40.0%
GATGAAGTAGAGGATGAAGA+CGG + chr7_4:18191942-18191961 Msa1142990:five_prime_UTR 40.0%
TGACTCTCAAGATTACGATG+AGG + chr7_4:18191989-18192008 Msa1142990:five_prime_UTR 40.0%
CACAGAACCTTGTAGACAAA+AGG - chr7_4:18192074-18192093 None:intergenic 40.0%
ATGAGGGTAAAGCATAGCAT+AGG + chr7_4:18192309-18192328 Msa1142990:CDS 40.0%
! CACAGGGCACTATATAAGTA+AGG - chr7_4:18192588-18192607 None:intergenic 40.0%
! AACCTGATTGGTCAATTGTG+TGG - chr7_4:18191148-18191167 None:intergenic 40.0%
GAGGATATCTCAAACCTGAT+TGG - chr7_4:18191160-18191179 None:intergenic 40.0%
TCTATACTAATTGGGAGGAG+AGG - chr7_4:18191179-18191198 None:intergenic 40.0%
! TGGGAGGAGATGATTTTGTA+TGG - chr7_4:18191216-18191235 None:intergenic 40.0%
ATGAAGACTGAACTATTGGG+AGG - chr7_4:18191232-18191251 None:intergenic 40.0%
AGAAACACAACCATTCCTTC+TGG + chr7_4:18191318-18191337 Msa1142990:five_prime_UTR 40.0%
GACTGTCATACAAATGAGGA+GGG - chr7_4:18191380-18191399 None:intergenic 40.0%
TGACTGTCATACAAATGAGG+AGG - chr7_4:18191381-18191400 None:intergenic 40.0%
TTTATCTCCATCGGAGTTGA+CGG - chr7_4:18191513-18191532 None:intergenic 40.0%
TTTACGAAATTCGTCGACGA+CGG + chr7_4:18191627-18191646 Msa1142990:five_prime_UTR 40.0%
!! GTGAATTTTAGTGATGGCAC+TGG + chr7_4:18191840-18191859 Msa1142990:intron 40.0%
ATTGAGCAACTCCTGAAACA+AGG + chr7_4:18191871-18191890 Msa1142990:five_prime_UTR 40.0%
TTGAGCAACTCCTGAAACAA+GGG + chr7_4:18191872-18191891 Msa1142990:five_prime_UTR 40.0%
! CACATTACTTCCCTTGTTTC+AGG - chr7_4:18191885-18191904 None:intergenic 40.0%
CTGAAACAAGGGAAGTAATG+TGG + chr7_4:18191883-18191902 Msa1142990:five_prime_UTR 40.0%
CACTAATGGAGATGAAGTAG+AGG + chr7_4:18191932-18191951 Msa1142990:five_prime_UTR 40.0%
GATGAAGTAGAGGATGAAGA+CGG + chr7_4:18191942-18191961 Msa1142990:five_prime_UTR 40.0%
TGACTCTCAAGATTACGATG+AGG + chr7_4:18191989-18192008 Msa1142990:five_prime_UTR 40.0%
CACAGAACCTTGTAGACAAA+AGG - chr7_4:18192074-18192093 None:intergenic 40.0%
ATGAGGGTAAAGCATAGCAT+AGG + chr7_4:18192309-18192328 Msa1142990:CDS 40.0%
! CACAGGGCACTATATAAGTA+AGG - chr7_4:18192588-18192607 None:intergenic 40.0%
! CTGATTGGTCAATTGTGTGG+TGG - chr7_4:18191145-18191164 None:intergenic 45.0%
CACCACACAATTGACCAATC+AGG + chr7_4:18191143-18191162 Msa1142990:five_prime_UTR 45.0%
GATCAACGAACCAGAAGGAA+TGG - chr7_4:18191331-18191350 None:intergenic 45.0%
TGTCATACAAATGAGGAGGG+AGG - chr7_4:18191377-18191396 None:intergenic 45.0%
TGTACTTTAATCAGCGGCGA+TGG - chr7_4:18191569-18191588 None:intergenic 45.0%
!! AAACAGTTGATGATGGTCGG+CGG + chr7_4:18191663-18191682 Msa1142990:five_prime_UTR 45.0%
GCATAGCATAGGAGCTTCAT+TGG + chr7_4:18192320-18192339 Msa1142990:CDS 45.0%
AAAATGGGGCGCAAATCACA+GGG - chr7_4:18192604-18192623 None:intergenic 45.0%
AAAAATGGGGCGCAAATCAC+AGG - chr7_4:18192605-18192624 None:intergenic 45.0%
!!! ATTTTGAGATTGGGGCTGCT+TGG + chr7_4:18192749-18192768 Msa1142990:CDS 45.0%
GTAGCCATATTGCCAACCAA+TGG + chr7_4:18192787-18192806 Msa1142990:CDS 45.0%
! ACCACCATTGGTTGGCAATA+TGG - chr7_4:18192794-18192813 None:intergenic 45.0%
TCGTTCAAACCACCATTGGT+TGG - chr7_4:18192802-18192821 None:intergenic 45.0%
! CTGATTGGTCAATTGTGTGG+TGG - chr7_4:18191145-18191164 None:intergenic 45.0%
CACCACACAATTGACCAATC+AGG + chr7_4:18191143-18191162 Msa1142990:five_prime_UTR 45.0%
GATCAACGAACCAGAAGGAA+TGG - chr7_4:18191331-18191350 None:intergenic 45.0%
TGTCATACAAATGAGGAGGG+AGG - chr7_4:18191377-18191396 None:intergenic 45.0%
TGTACTTTAATCAGCGGCGA+TGG - chr7_4:18191569-18191588 None:intergenic 45.0%
!! AAACAGTTGATGATGGTCGG+CGG + chr7_4:18191663-18191682 Msa1142990:five_prime_UTR 45.0%
GCATAGCATAGGAGCTTCAT+TGG + chr7_4:18192320-18192339 Msa1142990:CDS 45.0%
AAAATGGGGCGCAAATCACA+GGG - chr7_4:18192604-18192623 None:intergenic 45.0%
AAAAATGGGGCGCAAATCAC+AGG - chr7_4:18192605-18192624 None:intergenic 45.0%
!!! ATTTTGAGATTGGGGCTGCT+TGG + chr7_4:18192749-18192768 Msa1142990:CDS 45.0%
GTAGCCATATTGCCAACCAA+TGG + chr7_4:18192787-18192806 Msa1142990:CDS 45.0%
! ACCACCATTGGTTGGCAATA+TGG - chr7_4:18192794-18192813 None:intergenic 45.0%
TCGTTCAAACCACCATTGGT+TGG - chr7_4:18192802-18192821 None:intergenic 45.0%
CATACAAATGAGGAGGGAGG+TGG - chr7_4:18191374-18191393 None:intergenic 50.0%
GCCATATTGCCAACCAATGG+TGG + chr7_4:18192790-18192809 Msa1142990:CDS 50.0%
CATACAAATGAGGAGGGAGG+TGG - chr7_4:18191374-18191393 None:intergenic 50.0%
GCCATATTGCCAACCAATGG+TGG + chr7_4:18192790-18192809 Msa1142990:CDS 50.0%
CAACAGCCTAACTCCGATGC+CGG - chr7_4:18191447-18191466 None:intergenic 55.0%
TCTTCTTTCATCACCGCCGC+TGG + chr7_4:18191485-18191504 Msa1142990:five_prime_UTR 55.0%
! ATCGGAGTTGACGGATCCAG+CGG - chr7_4:18191504-18191523 None:intergenic 55.0%
ACGAAATTCGTCGACGACGG+CGG + chr7_4:18191630-18191649 Msa1142990:five_prime_UTR 55.0%
TCGGCGGACATCAGAGAAGA+AGG + chr7_4:18191679-18191698 Msa1142990:five_prime_UTR 55.0%
CAGTGAATGTTCCGCCGACA+TGG + chr7_4:18191715-18191734 Msa1142990:five_prime_UTR 55.0%
AGTAGAGGATGAAGACGGCG+AGG + chr7_4:18191947-18191966 Msa1142990:five_prime_UTR 55.0%
CAACAGCCTAACTCCGATGC+CGG - chr7_4:18191447-18191466 None:intergenic 55.0%
TCTTCTTTCATCACCGCCGC+TGG + chr7_4:18191485-18191504 Msa1142990:five_prime_UTR 55.0%
! ATCGGAGTTGACGGATCCAG+CGG - chr7_4:18191504-18191523 None:intergenic 55.0%
ACGAAATTCGTCGACGACGG+CGG + chr7_4:18191630-18191649 Msa1142990:five_prime_UTR 55.0%
TCGGCGGACATCAGAGAAGA+AGG + chr7_4:18191679-18191698 Msa1142990:five_prime_UTR 55.0%
CAGTGAATGTTCCGCCGACA+TGG + chr7_4:18191715-18191734 Msa1142990:five_prime_UTR 55.0%
AGTAGAGGATGAAGACGGCG+AGG + chr7_4:18191947-18191966 Msa1142990:five_prime_UTR 55.0%
ACCGCCACCGTCATCATCGT+CGG + chr7_4:18191419-18191438 Msa1142990:five_prime_UTR 60.0%
ACCGTCATCATCGTCGGGAC+CGG + chr7_4:18191425-18191444 Msa1142990:five_prime_UTR 60.0%
ATCATCGTCGGGACCGGCAT+CGG + chr7_4:18191431-18191450 Msa1142990:five_prime_UTR 60.0%
GCTGGATCCGTCAACTCCGA+TGG + chr7_4:18191503-18191522 Msa1142990:five_prime_UTR 60.0%
GGATGAAGACGGCGAGGATG+TGG + chr7_4:18191953-18191972 Msa1142990:five_prime_UTR 60.0%
ACCGCCACCGTCATCATCGT+CGG + chr7_4:18191419-18191438 Msa1142990:five_prime_UTR 60.0%
ACCGTCATCATCGTCGGGAC+CGG + chr7_4:18191425-18191444 Msa1142990:five_prime_UTR 60.0%
ATCATCGTCGGGACCGGCAT+CGG + chr7_4:18191431-18191450 Msa1142990:five_prime_UTR 60.0%
GCTGGATCCGTCAACTCCGA+TGG + chr7_4:18191503-18191522 Msa1142990:five_prime_UTR 60.0%
GGATGAAGACGGCGAGGATG+TGG + chr7_4:18191953-18191972 Msa1142990:five_prime_UTR 60.0%
ATGAGGAGGGAGGTGGTCGC+CGG - chr7_4:18191367-18191386 None:intergenic 65.0%
!! GACGATGATGACGGTGGCGG+TGG - chr7_4:18191420-18191439 None:intergenic 65.0%
!! CCCGACGATGATGACGGTGG+CGG - chr7_4:18191423-18191442 None:intergenic 65.0%
CCGCCACCGTCATCATCGTC+GGG + chr7_4:18191420-18191439 Msa1142990:five_prime_UTR 65.0%
!! GGTCCCGACGATGATGACGG+TGG - chr7_4:18191426-18191445 None:intergenic 65.0%
GCCGGTCCCGACGATGATGA+CGG - chr7_4:18191429-18191448 None:intergenic 65.0%
! TCGGGACCGGCATCGGAGTT+AGG + chr7_4:18191438-18191457 Msa1142990:five_prime_UTR 65.0%
! GGAGTTGACGGATCCAGCGG+CGG - chr7_4:18191501-18191520 None:intergenic 65.0%
ATGAGGAGGGAGGTGGTCGC+CGG - chr7_4:18191367-18191386 None:intergenic 65.0%
!! GACGATGATGACGGTGGCGG+TGG - chr7_4:18191420-18191439 None:intergenic 65.0%
!! CCCGACGATGATGACGGTGG+CGG - chr7_4:18191423-18191442 None:intergenic 65.0%
CCGCCACCGTCATCATCGTC+GGG + chr7_4:18191420-18191439 Msa1142990:five_prime_UTR 65.0%
!! GGTCCCGACGATGATGACGG+TGG - chr7_4:18191426-18191445 None:intergenic 65.0%
GCCGGTCCCGACGATGATGA+CGG - chr7_4:18191429-18191448 None:intergenic 65.0%
! TCGGGACCGGCATCGGAGTT+AGG + chr7_4:18191438-18191457 Msa1142990:five_prime_UTR 65.0%
! GGAGTTGACGGATCCAGCGG+CGG - chr7_4:18191501-18191520 None:intergenic 65.0%
AGGAGGGAGGTGGTCGCCGG+TGG - chr7_4:18191364-18191383 None:intergenic 75.0%
AGGAGGGAGGTGGTCGCCGG+TGG - chr7_4:18191364-18191383 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 18191132 18192828 18191132 ID=Msa1142990;Name=Msa1142990
chr7_4 mRNA 18191132 18192828 18191132 ID=Msa1142990-mRNA-1;Parent=Msa1142990;Name=Msa1142990-mRNA-1;_AED=0.41;_eAED=0.41;_QI=830|0.5|0.6|0.8|0.75|0.8|5|0|109
chr7_4 exon 18191132 18191753 18191132 ID=Msa1142990-mRNA-1:exon:4044;Parent=Msa1142990-mRNA-1
chr7_4 exon 18191851 18192010 18191851 ID=Msa1142990-mRNA-1:exon:4045;Parent=Msa1142990-mRNA-1
chr7_4 exon 18192168 18192357 18192168 ID=Msa1142990-mRNA-1:exon:4046;Parent=Msa1142990-mRNA-1
chr7_4 exon 18192523 18192578 18192523 ID=Msa1142990-mRNA-1:exon:4047;Parent=Msa1142990-mRNA-1
chr7_4 exon 18192697 18192828 18192697 ID=Msa1142990-mRNA-1:exon:4048;Parent=Msa1142990-mRNA-1
chr7_4 five_prime_UTR 18191132 18191753 18191132 ID=Msa1142990-mRNA-1:five_prime_utr;Parent=Msa1142990-mRNA-1
chr7_4 five_prime_UTR 18191851 18192010 18191851 ID=Msa1142990-mRNA-1:five_prime_utr;Parent=Msa1142990-mRNA-1
chr7_4 five_prime_UTR 18192168 18192215 18192168 ID=Msa1142990-mRNA-1:five_prime_utr;Parent=Msa1142990-mRNA-1
chr7_4 CDS 18192216 18192357 18192216 ID=Msa1142990-mRNA-1:cds;Parent=Msa1142990-mRNA-1
chr7_4 CDS 18192523 18192578 18192523 ID=Msa1142990-mRNA-1:cds;Parent=Msa1142990-mRNA-1
chr7_4 CDS 18192697 18192828 18192697 ID=Msa1142990-mRNA-1:cds;Parent=Msa1142990-mRNA-1
Gene Sequence

>Msa1142990

ATGATGTTATATGGGATGCTTTTGAAACTATTTGAGTTGTGGCTAAATTGCATATTTTTTTCTTCTCAAATCTTGAAATTTATATTGGTTGAAATGAGGGTAAAGCATAGCATAGGAGCTTCATTGGATGTGATTTTGACAGGTTGTCGCTTATCCGTGCATTCATATCCTATAACTTTGATTTCATATAGATTTAAGCATGAGTGTGATGATTTTGAAATTGACTTGGTGCTTGAGAATGAGTGTGATTATTTTGAGATTGGGGCTGCTTGGTGCTACTTTGCTTTTGTAGCCATATTGCCAACCAATGGTGGTTTGAACGAAAATTAG
Protein sequence

>Msa1142990

MMLYGMLLKLFELWLNCIFFSSQILKFILVEMRVKHSIGASLDVILTGCRLSVHSYPITLISYRFKHECDDFEIDLVLENECDYFEIGAAWCYFAFVAILPTNGGLNEN*