AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1156070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156070 MtrunA17_Chr8g0382051 66.667 90 30 0 1 90 1 90 7.41e-35 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156070 AT3G57360.1 50.000 90 37 3 1 90 1 82 1.68e-17 74.3

Find 83 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAAGATCAGAAGATTTA+AGG 0.117924 7_4:+44277584 None:intergenic
CATTCCATTGATAAGTTATT+TGG 0.155844 7_4:-44277514 Msa1156070:three_prime_UTR
GAAGCTTTCTTGCACATAAT+TGG 0.174578 7_4:+44276675 None:intergenic
CACAAGCACATGAGAAAATT+TGG 0.207694 7_4:+44277409 None:intergenic
CAATTGTTCAATTACGATAT+TGG 0.215158 7_4:-44276516 Msa1156070:three_prime_UTR
ACAATCCTGTATTTGCATTC+TGG 0.217227 7_4:-44276747 Msa1156070:three_prime_UTR
GATTTGGCACAGGTATCTTA+TGG 0.264509 7_4:-44277797 Msa1156070:three_prime_UTR
GTTTAAACCTCAACTGTAAA+AGG 0.266288 7_4:-44276644 Msa1156070:three_prime_UTR
AAAGTTGATGGTCGGAAGTT+TGG 0.269564 7_4:-44277709 Msa1156070:three_prime_UTR
CCTGGCCCAACTTGCAATTC+AGG 0.275772 7_4:-44278899 Msa1156070:intron
CAATCCTGTATTTGCATTCT+GGG 0.314898 7_4:-44276746 Msa1156070:three_prime_UTR
GTTCTTAAGGTATCATGTTC+AGG 0.321505 7_4:-44276431 Msa1156070:three_prime_UTR
GATGCAAAATTAGCCGAAAA+AGG 0.329205 7_4:-44277882 Msa1156070:three_prime_UTR
TTCAAGATCAGAAGATTTAA+GGG 0.330837 7_4:+44277585 None:intergenic
CTTAAATCTTCTGATCTTGA+AGG 0.344176 7_4:-44277583 Msa1156070:three_prime_UTR
GTAGCTCTTGAGAGAAAATA+TGG 0.344246 7_4:-44276862 Msa1156070:three_prime_UTR
GAACTATGGAAGAAAAGGTT+TGG 0.353007 7_4:-44279019 Msa1156070:exon
ACCATTATCTAATAGATCTA+AGG 0.353230 7_4:+44277765 None:intergenic
GGAAGATAAACATCAAAATC+TGG 0.353821 7_4:+44277466 None:intergenic
AAGCAGACAATTAGTAGATA+TGG 0.368214 7_4:-44277551 Msa1156070:three_prime_UTR
AACTATGGAAGAAAAGGTTT+GGG 0.368802 7_4:-44279018 Msa1156070:exon
GACTTTCTAAACCTCTTGTT+TGG 0.373355 7_4:+44277445 None:intergenic
AACAAACAAATTATTTAGTT+AGG 0.376642 7_4:-44277926 Msa1156070:three_prime_UTR
AAGGTGGAAGCGGCCGTGTT+GGG 0.393173 7_4:+44279957 None:intergenic
TTTCTCTATCTCTTCTCTCC+TGG 0.405905 7_4:-44279926 Msa1156070:intron
GCCTTAGATCTATTAGATAA+TGG 0.413209 7_4:-44277766 Msa1156070:three_prime_UTR
TTTGCGAGGTCACTGATAGA+AGG 0.426366 7_4:-44276822 Msa1156070:three_prime_UTR
CTTTACCTGAATTGCAAGTT+GGG 0.426982 7_4:+44278894 None:intergenic
GCTACAATGTCTGCATTAGA+TGG 0.433477 7_4:+44276880 None:intergenic
GTCGGAAGTTTGGATGTTGT+TGG 0.440117 7_4:-44277699 Msa1156070:three_prime_UTR
TTCGGCGGCATGGTGTTGCT+TGG 0.440523 7_4:-44277630 Msa1156070:three_prime_UTR
CAAGGTGGAAGCGGCCGTGT+TGG 0.443886 7_4:+44279956 None:intergenic
CATTCTGGGATGACATGAAA+AGG 0.444894 7_4:-44276732 Msa1156070:three_prime_UTR
AGTCTCTTGGCTACATAGTT+CGG 0.448906 7_4:-44277648 Msa1156070:three_prime_UTR
TGAAGCACACGTTGTGATCA+AGG 0.453286 7_4:+44276618 None:intergenic
CAAGATTTACACTTCAATTG+AGG 0.454593 7_4:-44278053 Msa1156070:CDS
GTCACCAAATAACTTATCAA+TGG 0.481135 7_4:+44277510 None:intergenic
CCTGAATTGCAAGTTGGGCC+AGG 0.481566 7_4:+44278899 None:intergenic
ACTTTACCTGAATTGCAAGT+TGG 0.486911 7_4:+44278893 None:intergenic
TCGGAAGTTTGGATGTTGTT+GGG 0.490686 7_4:-44277698 Msa1156070:three_prime_UTR
TGCAAGTTGGGCCAGGAAGT+TGG 0.490903 7_4:+44278906 None:intergenic
TTGCAAAATGCAAGTGATGA+TGG 0.492703 7_4:-44278948 Msa1156070:CDS
ATAGATTGAACAACATATGT+AGG 0.500909 7_4:+44276585 None:intergenic
CGTCCAACGAATTCGCAAGA+AGG 0.502794 7_4:+44276390 None:intergenic
TCATCCCAGAATGCAAATAC+AGG 0.506672 7_4:+44276742 None:intergenic
GGCAGATGGAATGAAGAGAT+GGG 0.508846 7_4:-44278139 Msa1156070:CDS
AATTATGTGCAAGAAAGCTT+CGG 0.515213 7_4:-44276673 Msa1156070:three_prime_UTR
AAGCGACAGCAAGGTGGAAG+CGG 0.516178 7_4:+44279947 None:intergenic
CGGCCGTGTTGGGTCCTCTA+CGG 0.518068 7_4:+44279967 None:intergenic
GCAGAAGTGTATGAAAAGAA+AGG 0.520348 7_4:-44278112 Msa1156070:CDS
GGCTACATAGTTCGGCGGCA+TGG 0.523179 7_4:-44277640 Msa1156070:three_prime_UTR
GTCCAACGAATTCGCAAGAA+GGG 0.523993 7_4:+44276391 None:intergenic
AGGCAGATGGAATGAAGAGA+TGG 0.524534 7_4:-44278140 Msa1156070:CDS
AGGTTTAGAAAGTCTTCTCC+TGG 0.525155 7_4:-44277436 Msa1156070:three_prime_UTR
GTGGCGGTGCTGTTTACAAA+GGG 0.532803 7_4:+44280048 None:intergenic
TATAGGCACCTCAAGTCTCT+TGG 0.537490 7_4:-44277661 Msa1156070:three_prime_UTR
CTACTAAAACGCCGCCGTAG+AGG 0.538981 7_4:-44279981 Msa1156070:five_prime_UTR
ATGTTTATCTTCCAAACAAG+AGG 0.541371 7_4:-44277456 Msa1156070:three_prime_UTR
ATGAGAAAATTTGGAATACC+AGG 0.550816 7_4:+44277418 None:intergenic
TGGTAAAATTTCACAACAGA+AGG 0.558169 7_4:+44277989 None:intergenic
TGCAAGAAAGCTTCGGATGG+TGG 0.559741 7_4:-44276666 Msa1156070:three_prime_UTR
ACACAAAATCGAAAGGCAGA+TGG 0.564175 7_4:-44278153 Msa1156070:CDS
ACAACTGGAATAGTTCGTAA+TGG 0.568611 7_4:-44278076 Msa1156070:CDS
ATGGAATGAAGAGATGGGAA+TGG 0.574705 7_4:-44278134 Msa1156070:CDS
AGATAGAGAAAGCGACAGCA+AGG 0.582580 7_4:+44279938 None:intergenic
GGTGGCGGTGCTGTTTACAA+AGG 0.585258 7_4:+44280047 None:intergenic
TAGATGTTCATCCTTGTCAA+AGG 0.597660 7_4:+44276786 None:intergenic
ATGTCTGCATTAGATGGAGG+AGG 0.599739 7_4:+44276886 None:intergenic
TCCAGGAACACAAAATCGAA+AGG 0.612133 7_4:-44278160 Msa1156070:intron
CATTCAACTGAAAATTTGCG+AGG 0.620493 7_4:-44276836 Msa1156070:three_prime_UTR
CTCTTGGCTACATAGTTCGG+CGG 0.631908 7_4:-44277645 Msa1156070:three_prime_UTR
TAAGTGAGAGAATGTGTTGG+TGG 0.633588 7_4:+44280029 None:intergenic
CCGCCGTAGAGGACCCAACA+CGG 0.644737 7_4:-44279970 Msa1156070:five_prime_UTR
TCCAACGAATTCGCAAGAAG+GGG 0.652296 7_4:+44276392 None:intergenic
CTATGTAGCCAAGAGACTTG+AGG 0.654136 7_4:+44277653 None:intergenic
ACAATGTCTGCATTAGATGG+AGG 0.656582 7_4:+44276883 None:intergenic
CCGTGTTGGGTCCTCTACGG+CGG 0.657313 7_4:+44279970 None:intergenic
ATATAAGTGAGAGAATGTGT+TGG 0.665180 7_4:+44280026 None:intergenic
GTGAGAGAATGTGTTGGTGG+CGG 0.665331 7_4:+44280032 None:intergenic
ATGTGCAAGAAAGCTTCGGA+TGG 0.666807 7_4:-44276669 Msa1156070:three_prime_UTR
TAGAGAAAGCGACAGCAAGG+TGG 0.669933 7_4:+44279941 None:intergenic
ATCAATGGAATGCTTGACTG+TGG 0.707373 7_4:+44277525 None:intergenic
GCTCTTGAGAGAAAATATGG+TGG 0.725754 7_4:-44276859 Msa1156070:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTTTTTTTTTAATT+TGG - chr7_4:44277819-44277838 Msa1156070:three_prime_UTR 0.0%
!! AACTATTAAGAAATATTTAA+AGG - chr7_4:44276776-44276795 Msa1156070:three_prime_UTR 10.0%
!!! TTATTTTTTACTAAATTTAC+AGG - chr7_4:44277933-44277952 Msa1156070:three_prime_UTR 10.0%
!!! TATTTTTTACTAAATTTACA+GGG - chr7_4:44277934-44277953 Msa1156070:three_prime_UTR 10.0%
!! TCTTGTATTTAATAAAGTTA+AGG - chr7_4:44276682-44276701 Msa1156070:three_prime_UTR 15.0%
!! AAAACTAACATTTATTATGA+GGG + chr7_4:44276966-44276985 None:intergenic 15.0%
!! ATTCAACATTTATTTATGAT+TGG - chr7_4:44277172-44277191 Msa1156070:intron 15.0%
!!! ATGGTTGATATTTTTTTTTT+AGG - chr7_4:44277587-44277606 Msa1156070:three_prime_UTR 15.0%
!!! GAGTTAATTTTAAGTTTAAA+CGG - chr7_4:44277684-44277703 Msa1156070:three_prime_UTR 15.0%
!! AACAAACAAATTATTTAGTT+AGG - chr7_4:44278520-44278539 Msa1156070:intron 15.0%
!!! ATTATTATGAATTCCTTTTT+CGG + chr7_4:44278580-44278599 None:intergenic 15.0%
!!! TTTTGGAAAATCTATTTAAT+CGG - chr7_4:44279510-44279529 Msa1156070:intron 15.0%
!! AATTGAAACATAATAACAGT+GGG - chr7_4:44276739-44276758 Msa1156070:three_prime_UTR 20.0%
!! TAATGAGTTAGATTTAAACT+TGG - chr7_4:44276800-44276819 Msa1156070:three_prime_UTR 20.0%
!! TATATGAATTAAATTGTGTC+AGG + chr7_4:44276841-44276860 None:intergenic 20.0%
!! TTTGTTTGATATTATACTCA+AGG - chr7_4:44276909-44276928 Msa1156070:intron 20.0%
!! CAAAACTAACATTTATTATG+AGG + chr7_4:44276967-44276986 None:intergenic 20.0%
!! TGTCTCTTTATAATAGAATT+AGG - chr7_4:44277012-44277031 Msa1156070:intron 20.0%
!! AATTGTTAACCAACAAAATA+GGG + chr7_4:44277339-44277358 None:intergenic 20.0%
!! TAATTGTTAACCAACAAAAT+AGG + chr7_4:44277340-44277359 None:intergenic 20.0%
!!! TATTTTGTTGGTTAACAATT+AGG - chr7_4:44277339-44277358 Msa1156070:intron 20.0%
!!! ATTTTGTTGGTTAACAATTA+GGG - chr7_4:44277340-44277359 Msa1156070:intron 20.0%
!!! TTTTTTTTTTAGGAGAACAA+TGG - chr7_4:44277597-44277616 Msa1156070:three_prime_UTR 20.0%
!!! ATGATGTGTATTTTTTTGTA+TGG - chr7_4:44277886-44277905 Msa1156070:three_prime_UTR 20.0%
!!! TTTTGTATGGATTTTTGTTT+AGG - chr7_4:44277899-44277918 Msa1156070:three_prime_UTR 20.0%
!! AGTAAAAAATAATCATAGAG+AGG + chr7_4:44277925-44277944 None:intergenic 20.0%
!!! CATAATAATACATTTTTGCT+AGG - chr7_4:44278591-44278610 Msa1156070:intron 20.0%
!! GAAATGTATAAAAAAGTTGA+TGG - chr7_4:44278725-44278744 Msa1156070:intron 20.0%
!! TCTTAATGAAAAGAAATGAA+AGG + chr7_4:44279321-44279340 None:intergenic 20.0%
!! AGTAAAAAATGAAAGAATGT+TGG + chr7_4:44279479-44279498 None:intergenic 20.0%
! AAAGAAATAAAACCTAAACC+TGG + chr7_4:44276617-44276636 None:intergenic 25.0%
!!! AGGTTTAGGTTTTATTTCTT+TGG - chr7_4:44276616-44276635 Msa1156070:three_prime_UTR 25.0%
! CAATTGAAACATAATAACAG+TGG - chr7_4:44276738-44276757 Msa1156070:three_prime_UTR 25.0%
!!! GATTTTCTAAAATTCTTGTG+TGG + chr7_4:44277224-44277243 None:intergenic 25.0%
! GAGTTTAAACAAACAATGAA+AGG + chr7_4:44277312-44277331 None:intergenic 25.0%
!! TCAATGGATTTATGAAAAGT+TGG + chr7_4:44277381-44277400 None:intergenic 25.0%
!! GCTGTAATTTGAAATATCAA+TGG + chr7_4:44277397-44277416 None:intergenic 25.0%
!! ATTTTGAACTATGGAAGAAA+AGG - chr7_4:44277422-44277441 Msa1156070:three_prime_UTR 25.0%
! TTAAATAAGCCAATCCATAT+AGG - chr7_4:44278180-44278199 Msa1156070:intron 25.0%
! ATGAAAAGAAAGGAAAAATG+TGG - chr7_4:44278344-44278363 Msa1156070:intron 25.0%
! TGAAAAGAAAGGAAAAATGT+GGG - chr7_4:44278345-44278364 Msa1156070:intron 25.0%
!!! TAGGATAAAGTTTTGAAAAC+TGG - chr7_4:44278610-44278629 Msa1156070:intron 25.0%
!!! TGCATATGTGAATTTTGATT+TGG - chr7_4:44278633-44278652 Msa1156070:intron 25.0%
! ACCATTATCTAATAGATCTA+AGG + chr7_4:44278684-44278703 None:intergenic 25.0%
!!! TGTATAAAAAAGTTGATGGT+CGG - chr7_4:44278729-44278748 Msa1156070:intron 25.0%
! TTCAAGATCAGAAGATTTAA+GGG + chr7_4:44278864-44278883 None:intergenic 25.0%
! CATTCCATTGATAAGTTATT+TGG - chr7_4:44278932-44278951 Msa1156070:CDS 25.0%
! CACATAAATTACCATAGAAA+TGG + chr7_4:44279254-44279273 None:intergenic 25.0%
! AAATGCCTAATAATTACTCA+AGG + chr7_4:44279288-44279307 None:intergenic 25.0%
!! TGAGTAATTATTAGGCATTT+TGG - chr7_4:44279288-44279307 Msa1156070:intron 25.0%
!!! TTTACTCCTTTATCTTGTTT+TGG - chr7_4:44279493-44279512 Msa1156070:intron 25.0%
!! GATTTTCCAAAACAAGATAA+AGG + chr7_4:44279502-44279521 None:intergenic 25.0%
! ATTGCATTTGTAATCTGTTA+GGG - chr7_4:44279535-44279554 Msa1156070:intron 25.0%
! ATAGATTGAACAACATATGT+AGG + chr7_4:44279864-44279883 None:intergenic 25.0%
! CAATTGTTCAATTACGATAT+TGG - chr7_4:44279930-44279949 Msa1156070:five_prime_UTR 25.0%
!!! AGCATCATTTTTAGTTCTTA+AGG - chr7_4:44280002-44280021 Msa1156070:five_prime_UTR 25.0%
ATATAAGTGAGAGAATGTGT+TGG + chr7_4:44276423-44276442 None:intergenic 30.0%
TAACATTTATTATGAGGGCT+CGG + chr7_4:44276961-44276980 None:intergenic 30.0%
ACTACTTAGAGAAAAAGAGT+TGG - chr7_4:44277052-44277071 Msa1156070:intron 30.0%
TTGGTTAACAATTAGGGAAA+AGG - chr7_4:44277346-44277365 Msa1156070:intron 30.0%
TGGTTAACAATTAGGGAAAA+GGG - chr7_4:44277347-44277366 Msa1156070:intron 30.0%
!!! CAGCTTTTGATTTTGAACTA+TGG - chr7_4:44277413-44277432 Msa1156070:three_prime_UTR 30.0%
AACTATGGAAGAAAAGGTTT+GGG - chr7_4:44277428-44277447 Msa1156070:three_prime_UTR 30.0%
!! TATTGATGATGATGATGATG+AGG - chr7_4:44277470-44277489 Msa1156070:three_prime_UTR 30.0%
GGTAAAGTATGTTTGAACAA+TGG - chr7_4:44277568-44277587 Msa1156070:three_prime_UTR 30.0%
TACTAAATTTACAGGGACTT+AGG - chr7_4:44277941-44277960 Msa1156070:three_prime_UTR 30.0%
ACTAAATTTACAGGGACTTA+GGG - chr7_4:44277942-44277961 Msa1156070:three_prime_UTR 30.0%
CTTATTTCCATAAACTCTCT+AGG + chr7_4:44278082-44278101 None:intergenic 30.0%
! CAGAGATATTTTCAGTACTA+AGG + chr7_4:44278206-44278225 None:intergenic 30.0%
!!! TTTTTTGCATTTCATCTTCC+AGG - chr7_4:44278269-44278288 Msa1156070:intron 30.0%
CAAGATTTACACTTCAATTG+AGG - chr7_4:44278393-44278412 Msa1156070:intron 30.0%
TGGTAAAATTTCACAACAGA+AGG + chr7_4:44278460-44278479 None:intergenic 30.0%
! TGTTGTGAAATTTTACCAGT+AGG - chr7_4:44278462-44278481 Msa1156070:intron 30.0%
! GTTGTGAAATTTTACCAGTA+GGG - chr7_4:44278463-44278482 Msa1156070:intron 30.0%
GCCTTAGATCTATTAGATAA+TGG - chr7_4:44278680-44278699 Msa1156070:intron 30.0%
CTTCAAGATCAGAAGATTTA+AGG + chr7_4:44278865-44278884 None:intergenic 30.0%
CTTAAATCTTCTGATCTTGA+AGG - chr7_4:44278863-44278882 Msa1156070:intron 30.0%
AAGCAGACAATTAGTAGATA+TGG - chr7_4:44278895-44278914 Msa1156070:intron 30.0%
GTCACCAAATAACTTATCAA+TGG + chr7_4:44278939-44278958 None:intergenic 30.0%
GGAAGATAAACATCAAAATC+TGG + chr7_4:44278983-44279002 None:intergenic 30.0%
ATGTTTATCTTCCAAACAAG+AGG - chr7_4:44278990-44279009 Msa1156070:CDS 30.0%
! ATGAGAAAATTTGGAATACC+AGG + chr7_4:44279031-44279050 None:intergenic 30.0%
TAAATTGTAATTTCGTAGCG+TGG + chr7_4:44279198-44279217 None:intergenic 30.0%
! AGATACAATTGCCATTTCTA+TGG - chr7_4:44279240-44279259 Msa1156070:intron 30.0%
GACTTCCTTGAGTAATTATT+AGG - chr7_4:44279280-44279299 Msa1156070:intron 30.0%
! TTTTCATTAAGAGTGATGTG+TGG - chr7_4:44279329-44279348 Msa1156070:intron 30.0%
CATTGCATTTGTAATCTGTT+AGG - chr7_4:44279534-44279553 Msa1156070:intron 30.0%
TTGCATTTGTAATCTGTTAG+GGG - chr7_4:44279536-44279555 Msa1156070:intron 30.0%
! AAGTTTCTTTTCCTTTGACA+AGG - chr7_4:44279649-44279668 Msa1156070:intron 30.0%
GGATTGTTACCAAAAATTCA+GGG + chr7_4:44279686-44279705 None:intergenic 30.0%
AGGATTGTTACCAAAAATTC+AGG + chr7_4:44279687-44279706 None:intergenic 30.0%
AATTATGTGCAAGAAAGCTT+CGG - chr7_4:44279773-44279792 Msa1156070:intron 30.0%
GTTTAAACCTCAACTGTAAA+AGG - chr7_4:44279802-44279821 Msa1156070:intron 30.0%
!!! AGTAGTTTTTTGTGGAAGCT+TGG + chr7_4:44276450-44276469 None:intergenic 35.0%
!! GGCGTTTTAGTAGTTTTTTG+TGG + chr7_4:44276458-44276477 None:intergenic 35.0%
! TCTTTGGAAGCTTTCAGAAT+AGG - chr7_4:44276632-44276651 Msa1156070:three_prime_UTR 35.0%
TGTTTCAATTGAACAACAGC+AGG + chr7_4:44276729-44276748 None:intergenic 35.0%
ATATAACTGACCCTATTGAG+TGG - chr7_4:44276856-44276875 Msa1156070:three_prime_UTR 35.0%
TATAACTGACCCTATTGAGT+GGG - chr7_4:44276857-44276876 Msa1156070:three_prime_UTR 35.0%
! AAGTCTTATCCCACTCAATA+GGG + chr7_4:44276869-44276888 None:intergenic 35.0%
! AAAGTCTTATCCCACTCAAT+AGG + chr7_4:44276870-44276889 None:intergenic 35.0%
AACATTTATTATGAGGGCTC+GGG + chr7_4:44276960-44276979 None:intergenic 35.0%
ACTTAGAGAAAAAGAGTTGG+AGG - chr7_4:44277055-44277074 Msa1156070:intron 35.0%
TTCTTGTGTGGTTTCACTAT+AGG + chr7_4:44277212-44277231 None:intergenic 35.0%
ACAAACAATGAAAGGAAGTG+AGG + chr7_4:44277304-44277323 None:intergenic 35.0%
! AACTCTGAACCCTATTTTGT+TGG - chr7_4:44277327-44277346 Msa1156070:intron 35.0%
GAACTATGGAAGAAAAGGTT+TGG - chr7_4:44277427-44277446 Msa1156070:three_prime_UTR 35.0%
TTGCAAAATGCAAGTGATGA+TGG - chr7_4:44277498-44277517 Msa1156070:three_prime_UTR 35.0%
CTTTACCTGAATTGCAAGTT+GGG + chr7_4:44277555-44277574 None:intergenic 35.0%
ACTTTACCTGAATTGCAAGT+TGG + chr7_4:44277556-44277575 None:intergenic 35.0%
! CACACAACTATAAGTTGAGA+AGG - chr7_4:44277654-44277673 Msa1156070:three_prime_UTR 35.0%
!! TGGAATCTTTAAAGAGTGTC+TGG - chr7_4:44277839-44277858 Msa1156070:three_prime_UTR 35.0%
!! GGAATCTTTAAAGAGTGTCT+GGG - chr7_4:44277840-44277859 Msa1156070:three_prime_UTR 35.0%
!! GAATCTTTAAAGAGTGTCTG+GGG - chr7_4:44277841-44277860 Msa1156070:three_prime_UTR 35.0%
CAAACATGCCTAAGTAACTA+AGG + chr7_4:44277976-44277995 None:intergenic 35.0%
CTTAGTTACTTAGGCATGTT+TGG - chr7_4:44277974-44277993 Msa1156070:three_prime_UTR 35.0%
AACCTATCCTAGAGAGTTTA+TGG - chr7_4:44278072-44278091 Msa1156070:CDS 35.0%
!! TTCCATAAACTCTCTAGGAT+AGG + chr7_4:44278077-44278096 None:intergenic 35.0%
AGACTGTTTGAAAGAGCTTA+AGG + chr7_4:44278133-44278152 None:intergenic 35.0%
TTTCAGTACTAAGGCCTATA+TGG + chr7_4:44278197-44278216 None:intergenic 35.0%
! GCAGAAGTGTATGAAAAGAA+AGG - chr7_4:44278334-44278353 Msa1156070:intron 35.0%
ACAACTGGAATAGTTCGTAA+TGG - chr7_4:44278370-44278389 Msa1156070:intron 35.0%
GATGCAAAATTAGCCGAAAA+AGG - chr7_4:44278564-44278583 Msa1156070:intron 35.0%
!!! TGTGAATTTTGATTTGGCAC+AGG - chr7_4:44278639-44278658 Msa1156070:intron 35.0%
GACTTTCTAAACCTCTTGTT+TGG + chr7_4:44279004-44279023 None:intergenic 35.0%
CACAAGCACATGAGAAAATT+TGG + chr7_4:44279040-44279059 None:intergenic 35.0%
! ATTTTCTCATGTGCTTGTGT+AGG - chr7_4:44279041-44279060 Msa1156070:intron 35.0%
ATGGCAATTGTATCTGACAA+AGG + chr7_4:44279235-44279254 None:intergenic 35.0%
! ATGTGTGGAGTCTATCTTTT+TGG - chr7_4:44279344-44279363 Msa1156070:intron 35.0%
! TCTATCTTTTTGGACCACTT+TGG - chr7_4:44279354-44279373 Msa1156070:intron 35.0%
ATGAAAGAATGTTGGAGCTA+CGG + chr7_4:44279471-44279490 None:intergenic 35.0%
GTAGCTCTTGAGAGAAAATA+TGG - chr7_4:44279584-44279603 Msa1156070:intron 35.0%
CATTCAACTGAAAATTTGCG+AGG - chr7_4:44279610-44279629 Msa1156070:intron 35.0%
TAGATGTTCATCCTTGTCAA+AGG + chr7_4:44279663-44279682 None:intergenic 35.0%
! GAACATCTACCCTGAATTTT+TGG - chr7_4:44279674-44279693 Msa1156070:intron 35.0%
ACAATCCTGTATTTGCATTC+TGG - chr7_4:44279699-44279718 Msa1156070:intron 35.0%
CAATCCTGTATTTGCATTCT+GGG - chr7_4:44279700-44279719 Msa1156070:intron 35.0%
GAAGCTTTCTTGCACATAAT+TGG + chr7_4:44279774-44279793 None:intergenic 35.0%
! AAGGATTCCTTTTACAGTTG+AGG + chr7_4:44279812-44279831 None:intergenic 35.0%
! GTTCTTAAGGTATCATGTTC+AGG - chr7_4:44280015-44280034 Msa1156070:five_prime_UTR 35.0%
TAAGTGAGAGAATGTGTTGG+TGG + chr7_4:44276420-44276439 None:intergenic 40.0%
TTTCTCTATCTCTTCTCTCC+TGG - chr7_4:44276520-44276539 Msa1156070:three_prime_UTR 40.0%
GGTTACAAAGAGAACTAACC+AGG + chr7_4:44276541-44276560 None:intergenic 40.0%
GTACAAGAGAGCGAAAAGAA+GGG + chr7_4:44276562-44276581 None:intergenic 40.0%
TGGTTGAAGACTCCTTCTTA+GGG + chr7_4:44276597-44276616 None:intergenic 40.0%
GAGAAAAAGAGTTGGAGGTT+TGG - chr7_4:44277060-44277079 Msa1156070:intron 40.0%
! TTGGAGGTTTGGTTGAAACT+TGG - chr7_4:44277071-44277090 Msa1156070:intron 40.0%
AATGAAAGGAAGTGAGGAAC+GGG + chr7_4:44277298-44277317 None:intergenic 40.0%
CAATGAAAGGAAGTGAGGAA+CGG + chr7_4:44277299-44277318 None:intergenic 40.0%
! TTGACTTTTCACCAACTTCC+TGG - chr7_4:44277529-44277548 Msa1156070:three_prime_UTR 40.0%
GAAATGCTAACGAGTACTCT+TGG - chr7_4:44277710-44277729 Msa1156070:three_prime_UTR 40.0%
TCACCATGCCTTAGTTACTT+AGG - chr7_4:44277965-44277984 Msa1156070:three_prime_UTR 40.0%
ATGCCTAAGTAACTAAGGCA+TGG + chr7_4:44277971-44277990 None:intergenic 40.0%
GTACTAAGGCCTATATGGAT+TGG + chr7_4:44278192-44278211 None:intergenic 40.0%
CAATGCTAAAGTGAAGCGAA+GGG + chr7_4:44278232-44278251 None:intergenic 40.0%
ACAATGCTAAAGTGAAGCGA+AGG + chr7_4:44278233-44278252 None:intergenic 40.0%
TCCAGGAACACAAAATCGAA+AGG - chr7_4:44278286-44278305 Msa1156070:intron 40.0%
ACACAAAATCGAAAGGCAGA+TGG - chr7_4:44278293-44278312 Msa1156070:intron 40.0%
ATGGAATGAAGAGATGGGAA+TGG - chr7_4:44278312-44278331 Msa1156070:intron 40.0%
GGAAAAATGTGGGTAACAAC+TGG - chr7_4:44278355-44278374 Msa1156070:intron 40.0%
GATTTGGCACAGGTATCTTA+TGG - chr7_4:44278649-44278668 Msa1156070:intron 40.0%
!! AAAGTTGATGGTCGGAAGTT+TGG - chr7_4:44278737-44278756 Msa1156070:intron 40.0%
TCGGAAGTTTGGATGTTGTT+GGG - chr7_4:44278748-44278767 Msa1156070:intron 40.0%
! ATGTTGTTGGGAGCTTTTTG+AGG - chr7_4:44278760-44278779 Msa1156070:intron 40.0%
AGTCTCTTGGCTACATAGTT+CGG - chr7_4:44278798-44278817 Msa1156070:intron 40.0%
ATCAATGGAATGCTTGACTG+TGG + chr7_4:44278924-44278943 None:intergenic 40.0%
AGGTTTAGAAAGTCTTCTCC+TGG - chr7_4:44279010-44279029 Msa1156070:CDS 40.0%
TGCACATAATGTGTCCAAAG+TGG + chr7_4:44279371-44279390 None:intergenic 40.0%
AAACGACTGAAAAATCAGCG+TGG + chr7_4:44279423-44279442 None:intergenic 40.0%
ACAATGTCTGCATTAGATGG+AGG + chr7_4:44279566-44279585 None:intergenic 40.0%
GCTACAATGTCTGCATTAGA+TGG + chr7_4:44279569-44279588 None:intergenic 40.0%
GCTCTTGAGAGAAAATATGG+TGG - chr7_4:44279587-44279606 Msa1156070:intron 40.0%
TCATCCCAGAATGCAAATAC+AGG + chr7_4:44279707-44279726 None:intergenic 40.0%
!! CATTCTGGGATGACATGAAA+AGG - chr7_4:44279714-44279733 Msa1156070:intron 40.0%
AGATAGAGAAAGCGACAGCA+AGG + chr7_4:44276511-44276530 None:intergenic 45.0%
CGTACAAGAGAGCGAAAAGA+AGG + chr7_4:44276563-44276582 None:intergenic 45.0%
GTTCAACACGAACCCTAAGA+AGG - chr7_4:44276582-44276601 Msa1156070:three_prime_UTR 45.0%
CTGGTTGAAGACTCCTTCTT+AGG + chr7_4:44276598-44276617 None:intergenic 45.0%
CTAAGAAGGAGTCTTCAACC+AGG - chr7_4:44276596-44276615 Msa1156070:three_prime_UTR 45.0%
AGGAGTCTTCAACCAGGTTT+AGG - chr7_4:44276602-44276621 Msa1156070:three_prime_UTR 45.0%
CGGAAATTTCAGAAACGCGA+GGG + chr7_4:44277273-44277292 None:intergenic 45.0%
TCGGAAATTTCAGAAACGCG+AGG + chr7_4:44277274-44277293 None:intergenic 45.0%
! GCCTTTCGATTTTGTGTTCC+TGG + chr7_4:44278290-44278309 None:intergenic 45.0%
AGGCAGATGGAATGAAGAGA+TGG - chr7_4:44278306-44278325 Msa1156070:intron 45.0%
GGCAGATGGAATGAAGAGAT+GGG - chr7_4:44278307-44278326 Msa1156070:intron 45.0%
! GTGCTGTGAATTTTCCCTAC+TGG + chr7_4:44278480-44278499 None:intergenic 45.0%
GTCGGAAGTTTGGATGTTGT+TGG - chr7_4:44278747-44278766 Msa1156070:intron 45.0%
!!! GGGAGCTTTTTGAGGCTTAT+AGG - chr7_4:44278768-44278787 Msa1156070:intron 45.0%
TATAGGCACCTCAAGTCTCT+TGG - chr7_4:44278785-44278804 Msa1156070:intron 45.0%
CTATGTAGCCAAGAGACTTG+AGG + chr7_4:44278796-44278815 None:intergenic 45.0%
ATGTCTGCATTAGATGGAGG+AGG + chr7_4:44279563-44279582 None:intergenic 45.0%
TTTGCGAGGTCACTGATAGA+AGG - chr7_4:44279624-44279643 Msa1156070:intron 45.0%
ATGTGCAAGAAAGCTTCGGA+TGG - chr7_4:44279777-44279796 Msa1156070:intron 45.0%
TGAAGCACACGTTGTGATCA+AGG + chr7_4:44279831-44279850 None:intergenic 45.0%
!! ATTAAAACTTATATTATTTA+AGG + chr7_4:44277751-44277770 None:intergenic 5.0%
! GTGAGAGAATGTGTTGGTGG+CGG + chr7_4:44276417-44276436 None:intergenic 50.0%
TAGAGAAAGCGACAGCAAGG+TGG + chr7_4:44276508-44276527 None:intergenic 50.0%
! AAGGAAGTGAGGAACGGGTT+CGG + chr7_4:44277293-44277312 None:intergenic 50.0%
TGCTAAAGTGAAGCGAAGGG+AGG + chr7_4:44278229-44278248 None:intergenic 50.0%
CTCTTGGCTACATAGTTCGG+CGG - chr7_4:44278801-44278820 Msa1156070:intron 50.0%
TGCAAGAAAGCTTCGGATGG+TGG - chr7_4:44279780-44279799 Msa1156070:intron 50.0%
CTACTAAAACGCCGCCGTAG+AGG - chr7_4:44276465-44276484 Msa1156070:three_prime_UTR 55.0%
AAGCGACAGCAAGGTGGAAG+CGG + chr7_4:44276502-44276521 None:intergenic 55.0%
! TGCAAGTTGGGCCAGGAAGT+TGG + chr7_4:44277543-44277562 None:intergenic 55.0%
! CCTGAATTGCAAGTTGGGCC+AGG + chr7_4:44277550-44277569 None:intergenic 55.0%
CCTGGCCCAACTTGCAATTC+AGG - chr7_4:44277547-44277566 Msa1156070:three_prime_UTR 55.0%
AAGGTGGAAGCGGCCGTGTT+GGG + chr7_4:44276492-44276511 None:intergenic 60.0%
GGCTACATAGTTCGGCGGCA+TGG - chr7_4:44278806-44278825 Msa1156070:intron 60.0%
!! TTCGGCGGCATGGTGTTGCT+TGG - chr7_4:44278816-44278835 Msa1156070:intron 60.0%
CCGTGTTGGGTCCTCTACGG+CGG + chr7_4:44276479-44276498 None:intergenic 65.0%
CCGCCGTAGAGGACCCAACA+CGG - chr7_4:44276476-44276495 Msa1156070:three_prime_UTR 65.0%
CGGCCGTGTTGGGTCCTCTA+CGG + chr7_4:44276482-44276501 None:intergenic 65.0%
CAAGGTGGAAGCGGCCGTGT+TGG + chr7_4:44276493-44276512 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 44276401 44280067 44276401 ID=Msa1156070;Name=Msa1156070
chr7_4 mRNA 44276401 44280067 44276401 ID=Msa1156070-mRNA-1;Parent=Msa1156070;Name=Msa1156070-mRNA-1;_AED=0.24;_eAED=0.24;_QI=157|1|1|1|0.66|0.25|4|1148|90
chr7_4 exon 44276401 44276912 44276401 ID=Msa1156070-mRNA-1:exon:11258;Parent=Msa1156070-mRNA-1
chr7_4 exon 44277406 44278177 44277406 ID=Msa1156070-mRNA-1:exon:11257;Parent=Msa1156070-mRNA-1
chr7_4 exon 44278900 44279052 44278900 ID=Msa1156070-mRNA-1:exon:11256;Parent=Msa1156070-mRNA-1
chr7_4 exon 44279927 44280067 44279927 ID=Msa1156070-mRNA-1:exon:11255;Parent=Msa1156070-mRNA-1
chr7_4 five_prime_UTR 44279927 44280067 44279927 ID=Msa1156070-mRNA-1:five_prime_utr;Parent=Msa1156070-mRNA-1
chr7_4 five_prime_UTR 44279037 44279052 44279037 ID=Msa1156070-mRNA-1:five_prime_utr;Parent=Msa1156070-mRNA-1
chr7_4 CDS 44278900 44279036 44278900 ID=Msa1156070-mRNA-1:cds;Parent=Msa1156070-mRNA-1
chr7_4 CDS 44278042 44278177 44278042 ID=Msa1156070-mRNA-1:cds;Parent=Msa1156070-mRNA-1
chr7_4 three_prime_UTR 44277406 44278041 44277406 ID=Msa1156070-mRNA-1:three_prime_utr;Parent=Msa1156070-mRNA-1
chr7_4 three_prime_UTR 44276401 44276912 44276401 ID=Msa1156070-mRNA-1:three_prime_utr;Parent=Msa1156070-mRNA-1
Gene Sequence

>Msa1156070

ATGGAAGAAAAGGTTTGGGAATGTTCTTTAAATGAAGATATTGATGATGATGATGATGAGGTCTATTTGCAAAATGCAAGTGATGATGGACAAGACTTTGACTTTTCACCAACTTCCTGGCCCAACTTGCAATTCAGGAACACAAAATCGAAAGGCAGATGGAATGAAGAGATGGGAATGGCAGAAGTGTATGAAAAGAAAGGAAAAATGTGGGTAACAACTGGAATAGTTCGTAATGGCAAGATTTACACTTCAATTGAGGAAACTTTGTAA
Protein sequence

>Msa1156070

MEEKVWECSLNEDIDDDDDEVYLQNASDDGQDFDFSPTSWPNLQFRNTKSKGRWNEEMGMAEVYEKKGKMWVTTGIVRNGKIYTSIEETL*