AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1157400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1157400 MtrunA17_Chr7g0251531 96.774 186 6 0 1 186 1 186 1.15e-133 371
Msa1157400 MtrunA17_Chr4g0066721 29.655 145 88 3 33 166 36 177 4.12e-16 72.8
Msa1157400 MtrunA17_Chr4g0021111 29.927 137 88 1 33 169 11 139 1.24e-15 70.9
Msa1157400 MtrunA17_Chr4g0042551 32.099 162 105 3 11 169 40 199 3.16e-14 68.2
Msa1157400 MtrunA17_Chr7g0268681 30.496 141 92 3 33 169 32 170 5.39e-14 67.0
Msa1157400 MtrunA17_Chr4g0066711 36.000 125 79 1 33 157 31 154 7.15e-13 63.9
Msa1157400 MtrunA17_Chr2g0286341 27.778 162 104 2 33 184 26 184 2.13e-12 62.8
Msa1157400 MtrunA17_Chr7g0217691 30.435 161 107 3 11 169 11 168 9.54e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1157400 AT4G25040.1 36.424 151 92 1 31 181 18 164 3.58e-21 85.9
Msa1157400 AT4G03540.1 34.959 123 72 1 47 169 26 140 6.48e-18 77.0
Msa1157400 AT4G15630.1 26.984 189 119 4 6 184 10 189 1.15e-15 72.0
Msa1157400 AT4G15620.1 30.435 161 100 3 34 184 31 189 3.50e-15 70.5
Msa1157400 AT1G03700.1 32.143 112 68 1 45 156 24 127 3.30e-14 67.4
Msa1157400 AT4G15610.1 28.105 153 105 2 10 157 9 161 4.30e-12 62.4
Msa1157400 AT5G15290.1 30.216 139 90 3 33 167 31 166 7.86e-12 61.6
Msa1157400 AT3G06390.1 25.455 165 108 5 5 157 9 170 4.28e-11 59.7

Find 48 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGCCTTCTTTGCCAATT+TGG 0.186565 7_4:-46382556 Msa1157400:CDS
TTTATATGAAATTTCTTATA+TGG 0.199631 7_4:-46382446 Msa1157400:three_prime_UTR
TTGGCCTTCTTTGCCAATTT+GGG 0.251825 7_4:-46382555 Msa1157400:CDS
AAAGTTCTGCAAAACCAATT+TGG 0.258540 7_4:-46382598 Msa1157400:CDS
TAAAATTGTGAGTCCCAAAT+TGG 0.269455 7_4:+46382542 None:intergenic
CATAGTTTAGAGTGAGGAAT+TGG 0.300862 7_4:+46384006 None:intergenic
TTCCATCCTGAAGGAATCTT+TGG 0.318409 7_4:+46383976 None:intergenic
AAGCAAAAGTGAAACCAAAT+TGG 0.326060 7_4:+46382584 None:intergenic
GGATGAGGTTGTTGAAGATT+TGG 0.339479 7_4:+46383952 None:intergenic
TTTAAATTCATTCTTCCTTA+AGG 0.364098 7_4:+46382343 None:intergenic
ACATCATAGGACTTTCTTCA+TGG 0.382860 7_4:-46383921 Msa1157400:CDS
CTGTTATGCTCTTCTCTATC+CGG 0.383937 7_4:-46383823 Msa1157400:CDS
AAGAGATGAAGCGAGTAGTA+TGG 0.390986 7_4:+46384183 None:intergenic
GATGAGGTTGTTGAAGATTT+GGG 0.392076 7_4:+46383953 None:intergenic
TAATTAAGAGGTGAACTTGT+AGG 0.396788 7_4:+46382515 None:intergenic
CAATTCCTCACTCTAAACTA+TGG 0.398146 7_4:-46384005 Msa1157400:exon
GTTGGATACGTGGGACAGTA+TGG 0.412618 7_4:-46382663 Msa1157400:CDS
GTAAAGTGAGAAGGACAAAA+GGG 0.429678 7_4:+46384107 None:intergenic
ATAGTTTAGAGTGAGGAATT+GGG 0.432294 7_4:+46384007 None:intergenic
CTGATGCCAAAGATTCCTTC+AGG 0.434784 7_4:-46383982 Msa1157400:CDS
TCAAAACATCCTCAGAATCT+TGG 0.440400 7_4:-46383897 Msa1157400:CDS
ATATGGAATCTATTTCTAGT+AGG 0.444026 7_4:-46382429 Msa1157400:three_prime_UTR
TACAATATACAACTACCTTA+AGG 0.453371 7_4:-46382358 Msa1157400:three_prime_UTR
AGTAAAGTGAGAAGGACAAA+AGG 0.457821 7_4:+46384106 None:intergenic
GAGAGGAGAAAATAATTAAG+AGG 0.459921 7_4:+46382503 None:intergenic
ATGACGGTGTTGCTGATAGC+TGG 0.468432 7_4:-46382705 Msa1157400:CDS
ATAAGAAATTTCATATAAAA+CGG 0.479727 7_4:+46382450 None:intergenic
ATGGAGAGAAACACGTTGGA+TGG 0.514689 7_4:-46382644 Msa1157400:CDS
GAGCATAACAGATTGGTTGT+TGG 0.526003 7_4:+46383835 None:intergenic
GAGTCCCAAATTGGCAAAGA+AGG 0.541836 7_4:+46382551 None:intergenic
AGGAGGAAAGTAAAGTGAGA+AGG 0.544239 7_4:+46384098 None:intergenic
TAGTTTAGAGTGAGGAATTG+GGG 0.549651 7_4:+46384008 None:intergenic
TAGAGAAGAGCATAACAGAT+TGG 0.552990 7_4:+46383828 None:intergenic
AGATTTGGGTTCCATCCTGA+AGG 0.555523 7_4:+46383967 None:intergenic
AGCCACAGCCGTTGGATACG+TGG 0.562855 7_4:-46382673 Msa1157400:CDS
CTCAATTTATCACATTCACA+TGG 0.574541 7_4:-46382483 Msa1157400:three_prime_UTR
TGTTCAGCAGCCACAGCCGT+TGG 0.589627 7_4:-46382681 Msa1157400:CDS
TCCCACGTATCCAACGGCTG+TGG 0.593429 7_4:+46382671 None:intergenic
TAAAGTGAGAAGGACAAAAG+GGG 0.594008 7_4:+46384108 None:intergenic
AAAAGTAAACAAAGACAACT+AGG 0.602388 7_4:+46384212 None:intergenic
TGCCAAAGATTCCTTCAGGA+TGG 0.603471 7_4:-46383978 Msa1157400:CDS
GAACAATGACCAAGATTCTG+AGG 0.616784 7_4:+46383888 None:intergenic
AGTTTAGAGTGAGGAATTGG+GGG 0.618942 7_4:+46384009 None:intergenic
ACAAAGACAACTAGGTAGCG+TGG 0.622944 7_4:+46384220 None:intergenic
ATACTGTCCCACGTATCCAA+CGG 0.624247 7_4:+46382665 None:intergenic
ATCAGCCATAGTTTAGAGTG+AGG 0.662471 7_4:+46384000 None:intergenic
TGTGAATGTGATAAATTGAG+AGG 0.675654 7_4:+46382486 None:intergenic
CAGTATGGAGAGAAACACGT+TGG 0.708078 7_4:-46382648 Msa1157400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGACATTATATTTTTTAT+AGG - chr7_4:46383499-46383518 Msa1157400:intron 10.0%
!!! ATGACATTATATTTTTTATA+GGG - chr7_4:46383500-46383519 Msa1157400:intron 10.0%
!! ATAAGAAATTTCATATAAAA+CGG + chr7_4:46384119-46384138 None:intergenic 10.0%
!! TTTATATGAAATTTCTTATA+TGG - chr7_4:46384120-46384139 Msa1157400:five_prime_UTR 10.0%
!!! AATGACATTATATTTTTTAT+AGG - chr7_4:46383499-46383518 Msa1157400:intron 10.0%
!!! ATGACATTATATTTTTTATA+GGG - chr7_4:46383500-46383519 Msa1157400:intron 10.0%
!! ATAAGAAATTTCATATAAAA+CGG + chr7_4:46384119-46384138 None:intergenic 10.0%
!! TTTATATGAAATTTCTTATA+TGG - chr7_4:46384120-46384139 Msa1157400:five_prime_UTR 10.0%
!! TTTGTTTATACATATGAAAA+TGG + chr7_4:46382834-46382853 None:intergenic 15.0%
!! TTCATATGTATAAACAAAAA+AGG - chr7_4:46382836-46382855 Msa1157400:intron 15.0%
!! TCATATGTATAAACAAAAAA+GGG - chr7_4:46382837-46382856 Msa1157400:intron 15.0%
!!! TGACATTATATTTTTTATAG+GGG - chr7_4:46383501-46383520 Msa1157400:intron 15.0%
!! TTCAATATAATTATACATGT+CGG + chr7_4:46383634-46383653 None:intergenic 15.0%
!!! TGATTTTCTTAAATTATTAG+CGG - chr7_4:46383659-46383678 Msa1157400:intron 15.0%
!! TTTGTTTATACATATGAAAA+TGG + chr7_4:46382834-46382853 None:intergenic 15.0%
!! TTCATATGTATAAACAAAAA+AGG - chr7_4:46382836-46382855 Msa1157400:intron 15.0%
!! TCATATGTATAAACAAAAAA+GGG - chr7_4:46382837-46382856 Msa1157400:intron 15.0%
!!! TGACATTATATTTTTTATAG+GGG - chr7_4:46383501-46383520 Msa1157400:intron 15.0%
!! TTCAATATAATTATACATGT+CGG + chr7_4:46383634-46383653 None:intergenic 15.0%
!!! TGATTTTCTTAAATTATTAG+CGG - chr7_4:46383659-46383678 Msa1157400:intron 15.0%
!! TACATATGAAAATGGAAAAT+GGG + chr7_4:46382826-46382845 None:intergenic 20.0%
!! ATACATATGAAAATGGAAAA+TGG + chr7_4:46382827-46382846 None:intergenic 20.0%
!!! TCTGTATTTATTTTTGTCTT+TGG - chr7_4:46382891-46382910 Msa1157400:intron 20.0%
!!! CTATTATTTCTTCCTATTTT+TGG - chr7_4:46383016-46383035 Msa1157400:intron 20.0%
!! ACTTGACTAAAACATTAAAT+GGG + chr7_4:46383391-46383410 None:intergenic 20.0%
!! AAACAATGACATGTATAATT+TGG + chr7_4:46383447-46383466 None:intergenic 20.0%
!! TTTAAATTCATTCTTCCTTA+AGG + chr7_4:46384226-46384245 None:intergenic 20.0%
!! TACATATGAAAATGGAAAAT+GGG + chr7_4:46382826-46382845 None:intergenic 20.0%
!! ATACATATGAAAATGGAAAA+TGG + chr7_4:46382827-46382846 None:intergenic 20.0%
!!! TCTGTATTTATTTTTGTCTT+TGG - chr7_4:46382891-46382910 Msa1157400:intron 20.0%
!!! CTATTATTTCTTCCTATTTT+TGG - chr7_4:46383016-46383035 Msa1157400:intron 20.0%
!! ACTTGACTAAAACATTAAAT+GGG + chr7_4:46383391-46383410 None:intergenic 20.0%
!! AAACAATGACATGTATAATT+TGG + chr7_4:46383447-46383466 None:intergenic 20.0%
!! TTTAAATTCATTCTTCCTTA+AGG + chr7_4:46384226-46384245 None:intergenic 20.0%
! AAAAGTAAACAAAGACAACT+AGG + chr7_4:46382357-46382376 None:intergenic 25.0%
!! AGAGCTTTACTAACTTTTAT+AGG + chr7_4:46382491-46382510 None:intergenic 25.0%
! AAAAAAACAGATGGAAATTG+AGG + chr7_4:46382524-46382543 None:intergenic 25.0%
! AAATGACACAAAAAAACAGA+TGG + chr7_4:46382533-46382552 None:intergenic 25.0%
! AATAAAAATGAGCTTCAAAC+CGG + chr7_4:46382765-46382784 None:intergenic 25.0%
!! TGATAATCAATCTTTTGATG+AGG - chr7_4:46382970-46382989 Msa1157400:intron 25.0%
!! AAATAATAGTCTTTTCGTTG+AGG + chr7_4:46383005-46383024 None:intergenic 25.0%
! TTACCAAATCAACCAAAAAT+AGG + chr7_4:46383031-46383050 None:intergenic 25.0%
!!! AAGCCAATAACATTTTAACA+TGG + chr7_4:46383125-46383144 None:intergenic 25.0%
!!! TTACTAGTGTCAATTTTAGT+GGG - chr7_4:46383304-46383323 Msa1157400:intron 25.0%
! GACTTGACTAAAACATTAAA+TGG + chr7_4:46383392-46383411 None:intergenic 25.0%
! ATACATGTCATTGTTTATCA+TGG - chr7_4:46383451-46383470 Msa1157400:intron 25.0%
!! GGAATATGATTGATAATATG+TGG - chr7_4:46383472-46383491 Msa1157400:intron 25.0%
! TTAGAAAGAAAGGTAAATGA+TGG + chr7_4:46383758-46383777 None:intergenic 25.0%
!!! ATTTTGTGGATTAGAAAGAA+AGG + chr7_4:46383768-46383787 None:intergenic 25.0%
! TCTTTCTAATCCACAAAATT+AGG - chr7_4:46383769-46383788 Msa1157400:intron 25.0%
!!! CATGTTTGTTATGAGAAAAT+TGG - chr7_4:46383821-46383840 Msa1157400:CDS 25.0%
!!! AAAATTGGTGAATTTTGATC+AGG - chr7_4:46383836-46383855 Msa1157400:CDS 25.0%
!!! TTCACTTTTGCTTTCATATT+TGG - chr7_4:46383992-46384011 Msa1157400:exon 25.0%
!!! ATATGGAATCTATTTCTAGT+AGG - chr7_4:46384137-46384156 Msa1157400:five_prime_UTR 25.0%
!!! AGTAGGAAATTTTTTCAGTT+TGG - chr7_4:46384154-46384173 Msa1157400:five_prime_UTR 25.0%
!!! AATTTTTTCAGTTTGGTGTA+TGG - chr7_4:46384161-46384180 Msa1157400:five_prime_UTR 25.0%
! TACAATATACAACTACCTTA+AGG - chr7_4:46384208-46384227 Msa1157400:five_prime_UTR 25.0%
! AAAAGTAAACAAAGACAACT+AGG + chr7_4:46382357-46382376 None:intergenic 25.0%
!! AGAGCTTTACTAACTTTTAT+AGG + chr7_4:46382491-46382510 None:intergenic 25.0%
! AAAAAAACAGATGGAAATTG+AGG + chr7_4:46382524-46382543 None:intergenic 25.0%
! AAATGACACAAAAAAACAGA+TGG + chr7_4:46382533-46382552 None:intergenic 25.0%
! AATAAAAATGAGCTTCAAAC+CGG + chr7_4:46382765-46382784 None:intergenic 25.0%
!! TGATAATCAATCTTTTGATG+AGG - chr7_4:46382970-46382989 Msa1157400:intron 25.0%
!! AAATAATAGTCTTTTCGTTG+AGG + chr7_4:46383005-46383024 None:intergenic 25.0%
! TTACCAAATCAACCAAAAAT+AGG + chr7_4:46383031-46383050 None:intergenic 25.0%
!!! AAGCCAATAACATTTTAACA+TGG + chr7_4:46383125-46383144 None:intergenic 25.0%
!!! TTACTAGTGTCAATTTTAGT+GGG - chr7_4:46383304-46383323 Msa1157400:intron 25.0%
! GACTTGACTAAAACATTAAA+TGG + chr7_4:46383392-46383411 None:intergenic 25.0%
! ATACATGTCATTGTTTATCA+TGG - chr7_4:46383451-46383470 Msa1157400:intron 25.0%
!! GGAATATGATTGATAATATG+TGG - chr7_4:46383472-46383491 Msa1157400:intron 25.0%
! TTAGAAAGAAAGGTAAATGA+TGG + chr7_4:46383758-46383777 None:intergenic 25.0%
!!! ATTTTGTGGATTAGAAAGAA+AGG + chr7_4:46383768-46383787 None:intergenic 25.0%
! TCTTTCTAATCCACAAAATT+AGG - chr7_4:46383769-46383788 Msa1157400:intron 25.0%
!!! CATGTTTGTTATGAGAAAAT+TGG - chr7_4:46383821-46383840 Msa1157400:CDS 25.0%
!!! AAAATTGGTGAATTTTGATC+AGG - chr7_4:46383836-46383855 Msa1157400:CDS 25.0%
!!! TTCACTTTTGCTTTCATATT+TGG - chr7_4:46383992-46384011 Msa1157400:exon 25.0%
!!! ATATGGAATCTATTTCTAGT+AGG - chr7_4:46384137-46384156 Msa1157400:five_prime_UTR 25.0%
!!! AGTAGGAAATTTTTTCAGTT+TGG - chr7_4:46384154-46384173 Msa1157400:five_prime_UTR 25.0%
!!! AATTTTTTCAGTTTGGTGTA+TGG - chr7_4:46384161-46384180 Msa1157400:five_prime_UTR 25.0%
! TACAATATACAACTACCTTA+AGG - chr7_4:46384208-46384227 Msa1157400:five_prime_UTR 25.0%
! GCTTTACTAACTTTTATAGG+AGG + chr7_4:46382488-46382507 None:intergenic 30.0%
! ATAGTTTAGAGTGAGGAATT+GGG + chr7_4:46382562-46382581 None:intergenic 30.0%
!!! AGATTTTTTGTTGCTGCAAA+TGG - chr7_4:46382918-46382937 Msa1157400:intron 30.0%
!!! CTTCCTATTTTTGGTTGATT+TGG - chr7_4:46383025-46383044 Msa1157400:intron 30.0%
GGTCCATGTTAAAATGTTAT+TGG - chr7_4:46383119-46383138 Msa1157400:intron 30.0%
!!! AATAGTTTTGAGTTAGACCT+TGG - chr7_4:46383248-46383267 Msa1157400:intron 30.0%
! CTTACTAGTGTCAATTTTAG+TGG - chr7_4:46383303-46383322 Msa1157400:intron 30.0%
TCTTAAATTATTAGCGGTGT+CGG - chr7_4:46383665-46383684 Msa1157400:intron 30.0%
AAAGTTCTGCAAAACCAATT+TGG - chr7_4:46383968-46383987 Msa1157400:CDS 30.0%
AAGCAAAAGTGAAACCAAAT+TGG + chr7_4:46383985-46384004 None:intergenic 30.0%
TAAAATTGTGAGTCCCAAAT+TGG + chr7_4:46384027-46384046 None:intergenic 30.0%
TAATTAAGAGGTGAACTTGT+AGG + chr7_4:46384054-46384073 None:intergenic 30.0%
GAGAGGAGAAAATAATTAAG+AGG + chr7_4:46384066-46384085 None:intergenic 30.0%
TGTGAATGTGATAAATTGAG+AGG + chr7_4:46384083-46384102 None:intergenic 30.0%
CTCAATTTATCACATTCACA+TGG - chr7_4:46384083-46384102 Msa1157400:five_prime_UTR 30.0%
! GCTTTACTAACTTTTATAGG+AGG + chr7_4:46382488-46382507 None:intergenic 30.0%
! ATAGTTTAGAGTGAGGAATT+GGG + chr7_4:46382562-46382581 None:intergenic 30.0%
!!! AGATTTTTTGTTGCTGCAAA+TGG - chr7_4:46382918-46382937 Msa1157400:intron 30.0%
!!! CTTCCTATTTTTGGTTGATT+TGG - chr7_4:46383025-46383044 Msa1157400:intron 30.0%
GGTCCATGTTAAAATGTTAT+TGG - chr7_4:46383119-46383138 Msa1157400:intron 30.0%
!!! AATAGTTTTGAGTTAGACCT+TGG - chr7_4:46383248-46383267 Msa1157400:intron 30.0%
! CTTACTAGTGTCAATTTTAG+TGG - chr7_4:46383303-46383322 Msa1157400:intron 30.0%
TCTTAAATTATTAGCGGTGT+CGG - chr7_4:46383665-46383684 Msa1157400:intron 30.0%
AAAGTTCTGCAAAACCAATT+TGG - chr7_4:46383968-46383987 Msa1157400:CDS 30.0%
AAGCAAAAGTGAAACCAAAT+TGG + chr7_4:46383985-46384004 None:intergenic 30.0%
TAAAATTGTGAGTCCCAAAT+TGG + chr7_4:46384027-46384046 None:intergenic 30.0%
TAATTAAGAGGTGAACTTGT+AGG + chr7_4:46384054-46384073 None:intergenic 30.0%
GAGAGGAGAAAATAATTAAG+AGG + chr7_4:46384066-46384085 None:intergenic 30.0%
TGTGAATGTGATAAATTGAG+AGG + chr7_4:46384083-46384102 None:intergenic 30.0%
CTCAATTTATCACATTCACA+TGG - chr7_4:46384083-46384102 Msa1157400:five_prime_UTR 30.0%
TAAAGTGAGAAGGACAAAAG+GGG + chr7_4:46382461-46382480 None:intergenic 35.0%
GTAAAGTGAGAAGGACAAAA+GGG + chr7_4:46382462-46382481 None:intergenic 35.0%
AGTAAAGTGAGAAGGACAAA+AGG + chr7_4:46382463-46382482 None:intergenic 35.0%
! TAGTTTAGAGTGAGGAATTG+GGG + chr7_4:46382561-46382580 None:intergenic 35.0%
! CATAGTTTAGAGTGAGGAAT+TGG + chr7_4:46382563-46382582 None:intergenic 35.0%
CAATTCCTCACTCTAAACTA+TGG - chr7_4:46382561-46382580 Msa1157400:CDS 35.0%
GATGAGGTTGTTGAAGATTT+GGG + chr7_4:46382616-46382635 None:intergenic 35.0%
!! ATGATGTTTTTGTGAGGATG+AGG + chr7_4:46382632-46382651 None:intergenic 35.0%
CATCCTCACAAAAACATCAT+AGG - chr7_4:46382632-46382651 Msa1157400:CDS 35.0%
!! AGTCCTATGATGTTTTTGTG+AGG + chr7_4:46382638-46382657 None:intergenic 35.0%
ACATCATAGGACTTTCTTCA+TGG - chr7_4:46382645-46382664 Msa1157400:CDS 35.0%
TCAAAACATCCTCAGAATCT+TGG - chr7_4:46382669-46382688 Msa1157400:CDS 35.0%
! TAGAGAAGAGCATAACAGAT+TGG + chr7_4:46382741-46382760 None:intergenic 35.0%
!! TGAAAATGGAAAATGGGTTG+TGG + chr7_4:46382820-46382839 None:intergenic 35.0%
! TTGGCTTAGACATGTTTTGT+CGG - chr7_4:46383138-46383157 Msa1157400:intron 35.0%
!!! GTCAATTTTAGTGGGTTAGT+CGG - chr7_4:46383312-46383331 Msa1157400:intron 35.0%
! TTCACAGATGTCTTTTACTC+TGG - chr7_4:46383603-46383622 Msa1157400:CDS 35.0%
TATCCGTATTCATGCTTCAT+AGG - chr7_4:46383720-46383739 Msa1157400:intron 35.0%
TGACCTATGAAGCATGAATA+CGG + chr7_4:46383726-46383745 None:intergenic 35.0%
! CTTATTCGCTCCTAATTTTG+TGG + chr7_4:46383782-46383801 None:intergenic 35.0%
!! GAATTTTGATCAGGTGATGA+CGG - chr7_4:46383845-46383864 Msa1157400:CDS 35.0%
! TTTTGCAGAACTTTTGCACA+TGG + chr7_4:46383962-46383981 None:intergenic 35.0%
TAAAGTGAGAAGGACAAAAG+GGG + chr7_4:46382461-46382480 None:intergenic 35.0%
GTAAAGTGAGAAGGACAAAA+GGG + chr7_4:46382462-46382481 None:intergenic 35.0%
AGTAAAGTGAGAAGGACAAA+AGG + chr7_4:46382463-46382482 None:intergenic 35.0%
! TAGTTTAGAGTGAGGAATTG+GGG + chr7_4:46382561-46382580 None:intergenic 35.0%
! CATAGTTTAGAGTGAGGAAT+TGG + chr7_4:46382563-46382582 None:intergenic 35.0%
CAATTCCTCACTCTAAACTA+TGG - chr7_4:46382561-46382580 Msa1157400:CDS 35.0%
GATGAGGTTGTTGAAGATTT+GGG + chr7_4:46382616-46382635 None:intergenic 35.0%
!! ATGATGTTTTTGTGAGGATG+AGG + chr7_4:46382632-46382651 None:intergenic 35.0%
CATCCTCACAAAAACATCAT+AGG - chr7_4:46382632-46382651 Msa1157400:CDS 35.0%
!! AGTCCTATGATGTTTTTGTG+AGG + chr7_4:46382638-46382657 None:intergenic 35.0%
ACATCATAGGACTTTCTTCA+TGG - chr7_4:46382645-46382664 Msa1157400:CDS 35.0%
TCAAAACATCCTCAGAATCT+TGG - chr7_4:46382669-46382688 Msa1157400:CDS 35.0%
! TAGAGAAGAGCATAACAGAT+TGG + chr7_4:46382741-46382760 None:intergenic 35.0%
!! TGAAAATGGAAAATGGGTTG+TGG + chr7_4:46382820-46382839 None:intergenic 35.0%
! TTGGCTTAGACATGTTTTGT+CGG - chr7_4:46383138-46383157 Msa1157400:intron 35.0%
!!! GTCAATTTTAGTGGGTTAGT+CGG - chr7_4:46383312-46383331 Msa1157400:intron 35.0%
! TTCACAGATGTCTTTTACTC+TGG - chr7_4:46383603-46383622 Msa1157400:CDS 35.0%
TATCCGTATTCATGCTTCAT+AGG - chr7_4:46383720-46383739 Msa1157400:intron 35.0%
TGACCTATGAAGCATGAATA+CGG + chr7_4:46383726-46383745 None:intergenic 35.0%
! CTTATTCGCTCCTAATTTTG+TGG + chr7_4:46383782-46383801 None:intergenic 35.0%
!! GAATTTTGATCAGGTGATGA+CGG - chr7_4:46383845-46383864 Msa1157400:CDS 35.0%
! TTTTGCAGAACTTTTGCACA+TGG + chr7_4:46383962-46383981 None:intergenic 35.0%
AAGAGATGAAGCGAGTAGTA+TGG + chr7_4:46382386-46382405 None:intergenic 40.0%
AGGAGGAAAGTAAAGTGAGA+AGG + chr7_4:46382471-46382490 None:intergenic 40.0%
! AGTTTAGAGTGAGGAATTGG+GGG + chr7_4:46382560-46382579 None:intergenic 40.0%
! ATCAGCCATAGTTTAGAGTG+AGG + chr7_4:46382569-46382588 None:intergenic 40.0%
TTCCATCCTGAAGGAATCTT+TGG + chr7_4:46382593-46382612 None:intergenic 40.0%
GGATGAGGTTGTTGAAGATT+TGG + chr7_4:46382617-46382636 None:intergenic 40.0%
GAACAATGACCAAGATTCTG+AGG + chr7_4:46382681-46382700 None:intergenic 40.0%
! GAGCATAACAGATTGGTTGT+TGG + chr7_4:46382734-46382753 None:intergenic 40.0%
CTGTTATGCTCTTCTCTATC+CGG - chr7_4:46382743-46382762 Msa1157400:intron 40.0%
! TTTTGTTGCTGCAAATGGTG+TGG - chr7_4:46382923-46382942 Msa1157400:intron 40.0%
TGTCTGAAAACAGAAATCGC+AGG - chr7_4:46383098-46383117 Msa1157400:intron 40.0%
GAGTTGTAACCTACTTAGGT+TGG - chr7_4:46383217-46383236 Msa1157400:intron 40.0%
!! GTAACCTACTTAGGTTGGTT+TGG - chr7_4:46383222-46383241 Msa1157400:intron 40.0%
TCAACCAAACCAACCTAAGT+AGG + chr7_4:46383229-46383248 None:intergenic 40.0%
! TTTGAGAGGAGTATACTCCA+AGG + chr7_4:46383268-46383287 None:intergenic 40.0%
ATTGAAGTCGAGACTTTGAG+AGG + chr7_4:46383282-46383301 None:intergenic 40.0%
TGGTTAATTACCTCAGCACT+AGG + chr7_4:46383427-46383446 None:intergenic 40.0%
GACAGTAGCTATGTTATGTC+TGG - chr7_4:46383691-46383710 Msa1157400:intron 40.0%
! TTTGGCCTTCTTTGCCAATT+TGG - chr7_4:46384010-46384029 Msa1157400:five_prime_UTR 40.0%
! TTGGCCTTCTTTGCCAATTT+GGG - chr7_4:46384011-46384030 Msa1157400:five_prime_UTR 40.0%
AAGAGATGAAGCGAGTAGTA+TGG + chr7_4:46382386-46382405 None:intergenic 40.0%
AGGAGGAAAGTAAAGTGAGA+AGG + chr7_4:46382471-46382490 None:intergenic 40.0%
! AGTTTAGAGTGAGGAATTGG+GGG + chr7_4:46382560-46382579 None:intergenic 40.0%
! ATCAGCCATAGTTTAGAGTG+AGG + chr7_4:46382569-46382588 None:intergenic 40.0%
TTCCATCCTGAAGGAATCTT+TGG + chr7_4:46382593-46382612 None:intergenic 40.0%
GGATGAGGTTGTTGAAGATT+TGG + chr7_4:46382617-46382636 None:intergenic 40.0%
GAACAATGACCAAGATTCTG+AGG + chr7_4:46382681-46382700 None:intergenic 40.0%
! GAGCATAACAGATTGGTTGT+TGG + chr7_4:46382734-46382753 None:intergenic 40.0%
CTGTTATGCTCTTCTCTATC+CGG - chr7_4:46382743-46382762 Msa1157400:intron 40.0%
! TTTTGTTGCTGCAAATGGTG+TGG - chr7_4:46382923-46382942 Msa1157400:intron 40.0%
TGTCTGAAAACAGAAATCGC+AGG - chr7_4:46383098-46383117 Msa1157400:intron 40.0%
GAGTTGTAACCTACTTAGGT+TGG - chr7_4:46383217-46383236 Msa1157400:intron 40.0%
!! GTAACCTACTTAGGTTGGTT+TGG - chr7_4:46383222-46383241 Msa1157400:intron 40.0%
TCAACCAAACCAACCTAAGT+AGG + chr7_4:46383229-46383248 None:intergenic 40.0%
! TTTGAGAGGAGTATACTCCA+AGG + chr7_4:46383268-46383287 None:intergenic 40.0%
ATTGAAGTCGAGACTTTGAG+AGG + chr7_4:46383282-46383301 None:intergenic 40.0%
TGGTTAATTACCTCAGCACT+AGG + chr7_4:46383427-46383446 None:intergenic 40.0%
GACAGTAGCTATGTTATGTC+TGG - chr7_4:46383691-46383710 Msa1157400:intron 40.0%
! TTTGGCCTTCTTTGCCAATT+TGG - chr7_4:46384010-46384029 Msa1157400:five_prime_UTR 40.0%
! TTGGCCTTCTTTGCCAATTT+GGG - chr7_4:46384011-46384030 Msa1157400:five_prime_UTR 40.0%
ACAAAGACAACTAGGTAGCG+TGG + chr7_4:46382349-46382368 None:intergenic 45.0%
CTGATGCCAAAGATTCCTTC+AGG - chr7_4:46382584-46382603 Msa1157400:CDS 45.0%
TGCCAAAGATTCCTTCAGGA+TGG - chr7_4:46382588-46382607 Msa1157400:CDS 45.0%
! AGATTTGGGTTCCATCCTGA+AGG + chr7_4:46382602-46382621 None:intergenic 45.0%
CGCAGAGTTGTAACCTACTT+AGG - chr7_4:46383213-46383232 Msa1157400:intron 45.0%
TTACCTCAGCACTAGGCAAA+CGG + chr7_4:46383420-46383439 None:intergenic 45.0%
ATACTGTCCCACGTATCCAA+CGG + chr7_4:46383904-46383923 None:intergenic 45.0%
CAGTATGGAGAGAAACACGT+TGG - chr7_4:46383918-46383937 Msa1157400:CDS 45.0%
ATGGAGAGAAACACGTTGGA+TGG - chr7_4:46383922-46383941 Msa1157400:CDS 45.0%
GAGTCCCAAATTGGCAAAGA+AGG + chr7_4:46384018-46384037 None:intergenic 45.0%
ACAAAGACAACTAGGTAGCG+TGG + chr7_4:46382349-46382368 None:intergenic 45.0%
CTGATGCCAAAGATTCCTTC+AGG - chr7_4:46382584-46382603 Msa1157400:CDS 45.0%
TGCCAAAGATTCCTTCAGGA+TGG - chr7_4:46382588-46382607 Msa1157400:CDS 45.0%
! AGATTTGGGTTCCATCCTGA+AGG + chr7_4:46382602-46382621 None:intergenic 45.0%
CGCAGAGTTGTAACCTACTT+AGG - chr7_4:46383213-46383232 Msa1157400:intron 45.0%
TTACCTCAGCACTAGGCAAA+CGG + chr7_4:46383420-46383439 None:intergenic 45.0%
ATACTGTCCCACGTATCCAA+CGG + chr7_4:46383904-46383923 None:intergenic 45.0%
CAGTATGGAGAGAAACACGT+TGG - chr7_4:46383918-46383937 Msa1157400:CDS 45.0%
ATGGAGAGAAACACGTTGGA+TGG - chr7_4:46383922-46383941 Msa1157400:CDS 45.0%
GAGTCCCAAATTGGCAAAGA+AGG + chr7_4:46384018-46384037 None:intergenic 45.0%
!!! ATATTTTTTTTTATGTAAAT+CGG - chr7_4:46383180-46383199 Msa1157400:intron 5.0%
!!! ATATTTTTTTTTATGTAAAT+CGG - chr7_4:46383180-46383199 Msa1157400:intron 5.0%
!! ATGACGGTGTTGCTGATAGC+TGG - chr7_4:46383861-46383880 Msa1157400:CDS 50.0%
GTTGGATACGTGGGACAGTA+TGG - chr7_4:46383903-46383922 Msa1157400:CDS 50.0%
!! ATGACGGTGTTGCTGATAGC+TGG - chr7_4:46383861-46383880 Msa1157400:CDS 50.0%
GTTGGATACGTGGGACAGTA+TGG - chr7_4:46383903-46383922 Msa1157400:CDS 50.0%
TCTCCGTTTGCCTAGTGCTG+AGG - chr7_4:46383414-46383433 Msa1157400:intron 55.0%
TCTCCGTTTGCCTAGTGCTG+AGG - chr7_4:46383414-46383433 Msa1157400:intron 55.0%
TGTTCAGCAGCCACAGCCGT+TGG - chr7_4:46383885-46383904 Msa1157400:CDS 60.0%
AGCCACAGCCGTTGGATACG+TGG - chr7_4:46383893-46383912 Msa1157400:CDS 60.0%
GCCACAGCCGTTGGATACGT+GGG - chr7_4:46383894-46383913 Msa1157400:CDS 60.0%
TCCCACGTATCCAACGGCTG+TGG + chr7_4:46383898-46383917 None:intergenic 60.0%
TGTTCAGCAGCCACAGCCGT+TGG - chr7_4:46383885-46383904 Msa1157400:CDS 60.0%
AGCCACAGCCGTTGGATACG+TGG - chr7_4:46383893-46383912 Msa1157400:CDS 60.0%
GCCACAGCCGTTGGATACGT+GGG - chr7_4:46383894-46383913 Msa1157400:CDS 60.0%
TCCCACGTATCCAACGGCTG+TGG + chr7_4:46383898-46383917 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 46382339 46384249 46382339 ID=Msa1157400;Name=Msa1157400
chr7_4 mRNA 46382339 46384249 46382339 ID=Msa1157400-mRNA-1;Parent=Msa1157400;Name=Msa1157400-mRNA-1;_AED=0.11;_eAED=0.11;_QI=241|1|1|1|1|1|3|179|186
chr7_4 exon 46382339 46382730 46382339 ID=Msa1157400-mRNA-1:exon:12144;Parent=Msa1157400-mRNA-1
chr7_4 exon 46383542 46383668 46383542 ID=Msa1157400-mRNA-1:exon:12143;Parent=Msa1157400-mRNA-1
chr7_4 exon 46383788 46384249 46383788 ID=Msa1157400-mRNA-1:exon:12142;Parent=Msa1157400-mRNA-1
chr7_4 five_prime_UTR 46384009 46384249 46384009 ID=Msa1157400-mRNA-1:five_prime_utr;Parent=Msa1157400-mRNA-1
chr7_4 CDS 46383788 46384008 46383788 ID=Msa1157400-mRNA-1:cds;Parent=Msa1157400-mRNA-1
chr7_4 CDS 46383542 46383668 46383542 ID=Msa1157400-mRNA-1:cds;Parent=Msa1157400-mRNA-1
chr7_4 CDS 46382518 46382730 46382518 ID=Msa1157400-mRNA-1:cds;Parent=Msa1157400-mRNA-1
chr7_4 three_prime_UTR 46382339 46382517 46382339 ID=Msa1157400-mRNA-1:three_prime_utr;Parent=Msa1157400-mRNA-1
Gene Sequence

>Msa1157400

ATGGCTGATGCCAAAGATTCCTTCAGGATGGAACCCAAATCTTCAACAACCTCATCCTCACAAAAACATCATAGGACTTTCTTCATGGCTCAAAACATCCTCAGAATCTTGGTCATTGTTCTAACTGCTGTTTCAATTGTAGTTACTGTCACCAACAACCAATCTGTTATGCTCTTCTCTATCCGGTTTGAAGCTCATTTTTATTACACATCATCTTTCAAATTTTTTGTTGCTGCAAATGGTGTGGTCTGTTTCATGTCTGTGCTGACACTGATAATCAATCTTTTGATGAGGCAACAAACACCTCAACGAAAAGACTATTATTTCTTCCTATTTTTGGTTGATTTGGTGATGACGGTGTTGCTGATAGCTGGATGTTCAGCAGCCACAGCCGTTGGATACGTGGGACAGTATGGAGAGAAACACGTTGGATGGACTGCAATATGTGACCATGTGCAAAAGTTCTGCAAAACCAATTTGGTTTCACTTTTGCTTTCATATTTGGCCTTCTTTGCCAATTTGGGACTCACAATTTTAATAGCCTACAAGTTCACCTCTTAA
Protein sequence

>Msa1157400

MADAKDSFRMEPKSSTTSSSQKHHRTFFMAQNILRILVIVLTAVSIVVTVTNNQSVMLFSIRFEAHFYYTSSFKFFVAANGVVCFMSVLTLIINLLMRQQTPQRKDYYFFLFLVDLVMTVLLIAGCSAATAVGYVGQYGEKHVGWTAICDHVQKFCKTNLVSLLLSYLAFFANLGLTILIAYKFTS*