AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1160000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1160000 MtrunA17_Chr7g0256851 98.261 115 2 0 1 115 1 115 1.32e-80 231
Msa1160000 MtrunA17_Chr3g0085831 50.476 105 48 3 1 102 1 104 9.21e-30 105
Msa1160000 MtrunA17_Chr5g0437861 44.595 74 37 2 1 71 1 73 1.29e-15 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1160000 AT2G41430.5 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1160000 AT2G41430.4 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1160000 AT2G41430.2 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1160000 AT2G41430.1 65.000 80 24 2 1 76 1 80 2.55e-30 106
Msa1160000 AT2G41430.3 65.000 80 24 2 1 76 1 80 4.23e-30 104
Msa1160000 AT4G14270.1 57.407 54 23 0 2 55 3 56 2.36e-17 72.8
Msa1160000 AT4G14270.2 57.407 54 23 0 2 55 3 56 2.36e-17 72.8

Find 41 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACAGGTAAGAAAGTTTA+TGG 0.140300 7_4:+49739845 None:intergenic
AAGGAATTACCAGAGAAATT+TGG 0.241148 7_4:+49741121 None:intergenic
TTTCTTACCTGTCTTCAAAA+TGG 0.243639 7_4:-49739837 Msa1160000:three_prime_UTR
GTCACCAGCTGCCACCATTC+CGG 0.246976 7_4:+49740509 None:intergenic
TTCAAGATGGCATTAGTTTC+AGG 0.269984 7_4:-49740605 Msa1160000:exon
TTCCTTCTTACCAAAATCTA+TGG 0.308472 7_4:-49739997 Msa1160000:three_prime_UTR
TCACCAGCTGCCACCATTCC+GGG 0.312874 7_4:+49740510 None:intergenic
CTTTCTGTAACTTCTTGTAT+AGG 0.333956 7_4:-49740041 Msa1160000:three_prime_UTR
GGAATGTAAAGTGGTGCATT+AGG 0.338731 7_4:+49740563 None:intergenic
GACGGTCTTGCCTTAGCTTT+CGG 0.365977 7_4:-49740095 Msa1160000:three_prime_UTR
CAAGGAGCAAAGAAATTTAC+TGG 0.372256 7_4:-49740167 Msa1160000:CDS
AGTAAGTTTATTATTACAAT+TGG 0.386330 7_4:+49739718 None:intergenic
TGAAATGGTACCATGATTAC+TGG 0.394842 7_4:-49740481 Msa1160000:CDS
AGCCTTCATCATCATGTTGC+TGG 0.408811 7_4:+49740453 None:intergenic
ACCGTCGCATTCAGCGTCTA+TGG 0.413218 7_4:+49740112 None:intergenic
TTCTGAACATGTATCTTGTA+TGG 0.421871 7_4:+49739753 None:intergenic
GAATGTAAAGTGGTGCATTA+GGG 0.425842 7_4:+49740564 None:intergenic
AGACAGGTAAGAAAGTTTAT+GGG 0.447864 7_4:+49739846 None:intergenic
TCTCGCTAACCAAATTTCTC+TGG 0.450228 7_4:-49741130 Msa1160000:intron
TCGACGTAGCGAAATGCAGC+TGG 0.454423 7_4:+49740542 None:intergenic
TTCCCCGGAATGGTGGCAGC+TGG 0.476894 7_4:-49740513 Msa1160000:CDS
GCATCAAGATCAAACGCATC+AGG 0.498917 7_4:+49740380 None:intergenic
GCTGGCTAAGCCAGTAATCA+TGG 0.519480 7_4:+49740471 None:intergenic
ATTTACTGGATGAGCTAAAG+AGG 0.519766 7_4:-49740153 Msa1160000:exon
TGTAACTTCTTGTATAGGCA+AGG 0.531546 7_4:-49740036 Msa1160000:three_prime_UTR
TGACAGCACTCAGAAGTGTA+TGG 0.540019 7_4:-49739788 Msa1160000:three_prime_UTR
AGCTGGTGACAACACTGAAA+TGG 0.546239 7_4:-49740496 Msa1160000:CDS
GATGATGTAGATGAGTTTGA+CGG 0.549584 7_4:-49740425 Msa1160000:CDS
AGCCAGCAACATGATGATGA+AGG 0.560493 7_4:-49740455 Msa1160000:CDS
AGCTGCATTTCGCTACGTCG+AGG 0.567649 7_4:-49740540 Msa1160000:CDS
AGCTAAATAACCGAAAGCTA+AGG 0.582950 7_4:+49740085 None:intergenic
ACCATAGACGCTGAATGCGA+CGG 0.583100 7_4:-49740113 Msa1160000:three_prime_UTR
AGAGTTTATCAAGTCTTCTG+AGG 0.595138 7_4:-49740324 Msa1160000:CDS
CACCAGCTGCCACCATTCCG+GGG 0.612813 7_4:+49740511 None:intergenic
ATCAAGTCTTCTGAGGCACA+AGG 0.619098 7_4:-49740317 Msa1160000:intron
AGAGTGAAGGTGATTCAAGA+TGG 0.619356 7_4:-49740618 Msa1160000:exon
AAATCTATGGTAAAATAGAG+AGG 0.627618 7_4:-49739984 Msa1160000:three_prime_UTR
AAGATGGCATTAGTTTCAGG+AGG 0.635369 7_4:-49740602 Msa1160000:CDS
TGATGCGTTTGATCTTGATG+CGG 0.644823 7_4:-49740378 Msa1160000:CDS
AATGCAGCTGGAATGTAAAG+TGG 0.663728 7_4:+49740554 None:intergenic
ACAGAAAGTTAAAACTGACA+TGG 0.696360 7_4:+49740056 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTATAAGAAAAAAAAAAT+CGG + chr7_4:49739849-49739868 None:intergenic 10.0%
!!! TGATTAATTATTTTTACAAT+TGG + chr7_4:49740009-49740028 None:intergenic 10.0%
!! ATTTATGAAATTAATTGAAT+TGG - chr7_4:49740087-49740106 Msa1160000:three_prime_UTR 10.0%
!! AAGTATAAGAAAAAAAAAAT+CGG + chr7_4:49739849-49739868 None:intergenic 10.0%
!!! TGATTAATTATTTTTACAAT+TGG + chr7_4:49740009-49740028 None:intergenic 10.0%
!! ATTTATGAAATTAATTGAAT+TGG - chr7_4:49740087-49740106 Msa1160000:three_prime_UTR 10.0%
!!! TTGAATTTTTATTTCAGATC+TGG - chr7_4:49739935-49739954 Msa1160000:three_prime_UTR 20.0%
!!! CATTGTTAATTGTTGATTAA+TGG - chr7_4:49740200-49740219 Msa1160000:intron 20.0%
!!! TTGAATTTTTATTTCAGATC+TGG - chr7_4:49739935-49739954 Msa1160000:three_prime_UTR 20.0%
!!! CATTGTTAATTGTTGATTAA+TGG - chr7_4:49740200-49740219 Msa1160000:intron 20.0%
! TGAGTAATTGAAAGAGAAAA+AGG + chr7_4:49739765-49739784 None:intergenic 25.0%
! TTGATTAATGGTGTAGATTA+AGG - chr7_4:49740212-49740231 Msa1160000:intron 25.0%
!! AACTTTCCTCTGAAAAATAA+CGG + chr7_4:49740669-49740688 None:intergenic 25.0%
!! CTCTATTTTACCATAGATTT+TGG + chr7_4:49740880-49740899 None:intergenic 25.0%
! AAATCTATGGTAAAATAGAG+AGG - chr7_4:49740880-49740899 Msa1160000:intron 25.0%
! TGAGTAATTGAAAGAGAAAA+AGG + chr7_4:49739765-49739784 None:intergenic 25.0%
! TTGATTAATGGTGTAGATTA+AGG - chr7_4:49740212-49740231 Msa1160000:intron 25.0%
!! AACTTTCCTCTGAAAAATAA+CGG + chr7_4:49740669-49740688 None:intergenic 25.0%
!! CTCTATTTTACCATAGATTT+TGG + chr7_4:49740880-49740899 None:intergenic 25.0%
! AAATCTATGGTAAAATAGAG+AGG - chr7_4:49740880-49740899 Msa1160000:intron 25.0%
AAGGAATTACCAGAGAAATT+TGG + chr7_4:49739746-49739765 None:intergenic 30.0%
!! CAATTCTCTTTTGACTAGTT+TGG + chr7_4:49739912-49739931 None:intergenic 30.0%
!!! AACATACCGTTATTTTTCAG+AGG - chr7_4:49740660-49740679 Msa1160000:intron 30.0%
!! TTTTTCAGAGGAAAGTTTTG+TGG - chr7_4:49740672-49740691 Msa1160000:intron 30.0%
ACAGAAAGTTAAAACTGACA+TGG + chr7_4:49740811-49740830 None:intergenic 30.0%
CTTTCTGTAACTTCTTGTAT+AGG - chr7_4:49740823-49740842 Msa1160000:intron 30.0%
TTCCTTCTTACCAAAATCTA+TGG - chr7_4:49740867-49740886 Msa1160000:intron 30.0%
!! TACCATAGATTTTGGTAAGA+AGG + chr7_4:49740872-49740891 None:intergenic 30.0%
AGACAGGTAAGAAAGTTTAT+GGG + chr7_4:49741021-49741040 None:intergenic 30.0%
AAGACAGGTAAGAAAGTTTA+TGG + chr7_4:49741022-49741041 None:intergenic 30.0%
TTTCTTACCTGTCTTCAAAA+TGG - chr7_4:49741027-49741046 Msa1160000:intron 30.0%
TTCTGAACATGTATCTTGTA+TGG + chr7_4:49741114-49741133 None:intergenic 30.0%
AAGGAATTACCAGAGAAATT+TGG + chr7_4:49739746-49739765 None:intergenic 30.0%
!! CAATTCTCTTTTGACTAGTT+TGG + chr7_4:49739912-49739931 None:intergenic 30.0%
!!! AACATACCGTTATTTTTCAG+AGG - chr7_4:49740660-49740679 Msa1160000:intron 30.0%
!! TTTTTCAGAGGAAAGTTTTG+TGG - chr7_4:49740672-49740691 Msa1160000:intron 30.0%
ACAGAAAGTTAAAACTGACA+TGG + chr7_4:49740811-49740830 None:intergenic 30.0%
CTTTCTGTAACTTCTTGTAT+AGG - chr7_4:49740823-49740842 Msa1160000:intron 30.0%
TTCCTTCTTACCAAAATCTA+TGG - chr7_4:49740867-49740886 Msa1160000:intron 30.0%
!! TACCATAGATTTTGGTAAGA+AGG + chr7_4:49740872-49740891 None:intergenic 30.0%
AGACAGGTAAGAAAGTTTAT+GGG + chr7_4:49741021-49741040 None:intergenic 30.0%
AAGACAGGTAAGAAAGTTTA+TGG + chr7_4:49741022-49741041 None:intergenic 30.0%
TTTCTTACCTGTCTTCAAAA+TGG - chr7_4:49741027-49741046 Msa1160000:intron 30.0%
TTCTGAACATGTATCTTGTA+TGG + chr7_4:49741114-49741133 None:intergenic 30.0%
!! GACTAGTTTGGAATCAAGAT+CGG + chr7_4:49739900-49739919 None:intergenic 35.0%
! TTCAAGATGGCATTAGTTTC+AGG - chr7_4:49740259-49740278 Msa1160000:intron 35.0%
GAATGTAAAGTGGTGCATTA+GGG + chr7_4:49740303-49740322 None:intergenic 35.0%
TGAAATGGTACCATGATTAC+TGG - chr7_4:49740383-49740402 Msa1160000:CDS 35.0%
GATGATGTAGATGAGTTTGA+CGG - chr7_4:49740439-49740458 Msa1160000:CDS 35.0%
AGAGTTTATCAAGTCTTCTG+AGG - chr7_4:49740540-49740559 Msa1160000:CDS 35.0%
CAAGGAGCAAAGAAATTTAC+TGG - chr7_4:49740697-49740716 Msa1160000:intron 35.0%
ATTTACTGGATGAGCTAAAG+AGG - chr7_4:49740711-49740730 Msa1160000:intron 35.0%
AGCTAAATAACCGAAAGCTA+AGG + chr7_4:49740782-49740801 None:intergenic 35.0%
TGTAACTTCTTGTATAGGCA+AGG - chr7_4:49740828-49740847 Msa1160000:intron 35.0%
!! AATGCATCCATTTTGAAGAC+AGG + chr7_4:49741037-49741056 None:intergenic 35.0%
!! GACTAGTTTGGAATCAAGAT+CGG + chr7_4:49739900-49739919 None:intergenic 35.0%
! TTCAAGATGGCATTAGTTTC+AGG - chr7_4:49740259-49740278 Msa1160000:intron 35.0%
GAATGTAAAGTGGTGCATTA+GGG + chr7_4:49740303-49740322 None:intergenic 35.0%
TGAAATGGTACCATGATTAC+TGG - chr7_4:49740383-49740402 Msa1160000:CDS 35.0%
GATGATGTAGATGAGTTTGA+CGG - chr7_4:49740439-49740458 Msa1160000:CDS 35.0%
AGAGTTTATCAAGTCTTCTG+AGG - chr7_4:49740540-49740559 Msa1160000:CDS 35.0%
CAAGGAGCAAAGAAATTTAC+TGG - chr7_4:49740697-49740716 Msa1160000:intron 35.0%
ATTTACTGGATGAGCTAAAG+AGG - chr7_4:49740711-49740730 Msa1160000:intron 35.0%
AGCTAAATAACCGAAAGCTA+AGG + chr7_4:49740782-49740801 None:intergenic 35.0%
TGTAACTTCTTGTATAGGCA+AGG - chr7_4:49740828-49740847 Msa1160000:intron 35.0%
!! AATGCATCCATTTTGAAGAC+AGG + chr7_4:49741037-49741056 None:intergenic 35.0%
TCTCGCTAACCAAATTTCTC+TGG - chr7_4:49739734-49739753 Msa1160000:three_prime_UTR 40.0%
GGAAAAAGTGACAAGAGTGA+AGG - chr7_4:49740233-49740252 Msa1160000:intron 40.0%
AGAGTGAAGGTGATTCAAGA+TGG - chr7_4:49740246-49740265 Msa1160000:intron 40.0%
! AAGATGGCATTAGTTTCAGG+AGG - chr7_4:49740262-49740281 Msa1160000:intron 40.0%
GGAATGTAAAGTGGTGCATT+AGG + chr7_4:49740304-49740323 None:intergenic 40.0%
AATGCAGCTGGAATGTAAAG+TGG + chr7_4:49740313-49740332 None:intergenic 40.0%
TGATGCGTTTGATCTTGATG+CGG - chr7_4:49740486-49740505 Msa1160000:CDS 40.0%
TCTCGCTAACCAAATTTCTC+TGG - chr7_4:49739734-49739753 Msa1160000:three_prime_UTR 40.0%
GGAAAAAGTGACAAGAGTGA+AGG - chr7_4:49740233-49740252 Msa1160000:intron 40.0%
AGAGTGAAGGTGATTCAAGA+TGG - chr7_4:49740246-49740265 Msa1160000:intron 40.0%
! AAGATGGCATTAGTTTCAGG+AGG - chr7_4:49740262-49740281 Msa1160000:intron 40.0%
GGAATGTAAAGTGGTGCATT+AGG + chr7_4:49740304-49740323 None:intergenic 40.0%
AATGCAGCTGGAATGTAAAG+TGG + chr7_4:49740313-49740332 None:intergenic 40.0%
TGATGCGTTTGATCTTGATG+CGG - chr7_4:49740486-49740505 Msa1160000:CDS 40.0%
AGCTGGTGACAACACTGAAA+TGG - chr7_4:49740368-49740387 Msa1160000:CDS 45.0%
! AGCCAGCAACATGATGATGA+AGG - chr7_4:49740409-49740428 Msa1160000:CDS 45.0%
AGCCTTCATCATCATGTTGC+TGG + chr7_4:49740414-49740433 None:intergenic 45.0%
GCATCAAGATCAAACGCATC+AGG + chr7_4:49740487-49740506 None:intergenic 45.0%
!! ATCAAGTCTTCTGAGGCACA+AGG - chr7_4:49740547-49740566 Msa1160000:CDS 45.0%
!! GAGGAAAGTTTTGTGGAGCA+AGG - chr7_4:49740679-49740698 Msa1160000:intron 45.0%
! TGACAGCACTCAGAAGTGTA+TGG - chr7_4:49741076-49741095 Msa1160000:intron 45.0%
AGCTGGTGACAACACTGAAA+TGG - chr7_4:49740368-49740387 Msa1160000:CDS 45.0%
! AGCCAGCAACATGATGATGA+AGG - chr7_4:49740409-49740428 Msa1160000:CDS 45.0%
AGCCTTCATCATCATGTTGC+TGG + chr7_4:49740414-49740433 None:intergenic 45.0%
GCATCAAGATCAAACGCATC+AGG + chr7_4:49740487-49740506 None:intergenic 45.0%
!! ATCAAGTCTTCTGAGGCACA+AGG - chr7_4:49740547-49740566 Msa1160000:CDS 45.0%
!! GAGGAAAGTTTTGTGGAGCA+AGG - chr7_4:49740679-49740698 Msa1160000:intron 45.0%
! TGACAGCACTCAGAAGTGTA+TGG - chr7_4:49741076-49741095 Msa1160000:intron 45.0%
! CTACGTCGAGGATTTTTCCC+CGG - chr7_4:49740336-49740355 Msa1160000:CDS 50.0%
! TCGAGGATTTTTCCCCGGAA+TGG - chr7_4:49740341-49740360 Msa1160000:CDS 50.0%
! AGGATTTTTCCCCGGAATGG+TGG - chr7_4:49740344-49740363 Msa1160000:CDS 50.0%
GCTGGCTAAGCCAGTAATCA+TGG + chr7_4:49740396-49740415 None:intergenic 50.0%
ACCATAGACGCTGAATGCGA+CGG - chr7_4:49740751-49740770 Msa1160000:intron 50.0%
! GACGGTCTTGCCTTAGCTTT+CGG - chr7_4:49740769-49740788 Msa1160000:intron 50.0%
! CTACGTCGAGGATTTTTCCC+CGG - chr7_4:49740336-49740355 Msa1160000:CDS 50.0%
! TCGAGGATTTTTCCCCGGAA+TGG - chr7_4:49740341-49740360 Msa1160000:CDS 50.0%
! AGGATTTTTCCCCGGAATGG+TGG - chr7_4:49740344-49740363 Msa1160000:CDS 50.0%
GCTGGCTAAGCCAGTAATCA+TGG + chr7_4:49740396-49740415 None:intergenic 50.0%
ACCATAGACGCTGAATGCGA+CGG - chr7_4:49740751-49740770 Msa1160000:intron 50.0%
! GACGGTCTTGCCTTAGCTTT+CGG - chr7_4:49740769-49740788 Msa1160000:intron 50.0%
TCGACGTAGCGAAATGCAGC+TGG + chr7_4:49740325-49740344 None:intergenic 55.0%
AGCTGCATTTCGCTACGTCG+AGG - chr7_4:49740324-49740343 Msa1160000:CDS 55.0%
ACCGTCGCATTCAGCGTCTA+TGG + chr7_4:49740755-49740774 None:intergenic 55.0%
TCGACGTAGCGAAATGCAGC+TGG + chr7_4:49740325-49740344 None:intergenic 55.0%
AGCTGCATTTCGCTACGTCG+AGG - chr7_4:49740324-49740343 Msa1160000:CDS 55.0%
ACCGTCGCATTCAGCGTCTA+TGG + chr7_4:49740755-49740774 None:intergenic 55.0%
TCACCAGCTGCCACCATTCC+GGG + chr7_4:49740357-49740376 None:intergenic 60.0%
GTCACCAGCTGCCACCATTC+CGG + chr7_4:49740358-49740377 None:intergenic 60.0%
TCACCAGCTGCCACCATTCC+GGG + chr7_4:49740357-49740376 None:intergenic 60.0%
GTCACCAGCTGCCACCATTC+CGG + chr7_4:49740358-49740377 None:intergenic 60.0%
TTCCCCGGAATGGTGGCAGC+TGG - chr7_4:49740351-49740370 Msa1160000:CDS 65.0%
CACCAGCTGCCACCATTCCG+GGG + chr7_4:49740356-49740375 None:intergenic 65.0%
TTCCCCGGAATGGTGGCAGC+TGG - chr7_4:49740351-49740370 Msa1160000:CDS 65.0%
CACCAGCTGCCACCATTCCG+GGG + chr7_4:49740356-49740375 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 49739720 49741166 49739720 ID=Msa1160000;Name=Msa1160000
chr7_4 mRNA 49739720 49741166 49739720 ID=Msa1160000-mRNA-1;Parent=Msa1160000;Name=Msa1160000-mRNA-1;_AED=0.00;_eAED=0.00;_QI=73|1|1|1|0.5|0.33|3|443|115
chr7_4 exon 49739720 49740206 49739720 ID=Msa1160000-mRNA-1:exon:13767;Parent=Msa1160000-mRNA-1
chr7_4 exon 49740318 49740658 49740318 ID=Msa1160000-mRNA-1:exon:13766;Parent=Msa1160000-mRNA-1
chr7_4 exon 49741131 49741166 49741131 ID=Msa1160000-mRNA-1:exon:13765;Parent=Msa1160000-mRNA-1
chr7_4 five_prime_UTR 49741131 49741166 49741131 ID=Msa1160000-mRNA-1:five_prime_utr;Parent=Msa1160000-mRNA-1
chr7_4 five_prime_UTR 49740622 49740658 49740622 ID=Msa1160000-mRNA-1:five_prime_utr;Parent=Msa1160000-mRNA-1
chr7_4 CDS 49740318 49740621 49740318 ID=Msa1160000-mRNA-1:cds;Parent=Msa1160000-mRNA-1
chr7_4 CDS 49740163 49740206 49740163 ID=Msa1160000-mRNA-1:cds;Parent=Msa1160000-mRNA-1
chr7_4 three_prime_UTR 49739720 49740162 49739720 ID=Msa1160000-mRNA-1:three_prime_utr;Parent=Msa1160000-mRNA-1
Gene Sequence

>Msa1160000

ATGGCATTAGTTTCAGGAGGAAGATCAACACTAAACCCTAATGCACCACTTTACATTCCAGCTGCATTTCGCTACGTCGAGGATTTTTCCCCGGAATGGTGGCAGCTGGTGACAACACTGAAATGGTACCATGATTACTGGCTTAGCCAGCAACATGATGATGAAGGCTTTTATGATGATGTAGATGAGTTTGACGGCAATGATGTCGTTGATTTGCTTCCTGATGCGTTTGATCTTGATGCGGATGAAGATCTTTCGATTGAAGCTCAGCTTGAAGAGTTTATCAAGTCTTCTGAGGCACAAGAGGAAAGTTTTGTGGAGCAAGGAGCAAAGAAATTTACTGGATGA

Protein sequence

>Msa1160000

MALVSGGRSTLNPNAPLYIPAAFRYVEDFSPEWWQLVTTLKWYHDYWLSQQHDDEGFYDDVDEFDGNDVVDLLPDAFDLDADEDLSIEAQLEEFIKSSEAQEESFVEQGAKKFTG*