Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1114040 | XP_003625072.1 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 2.92e-67 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1114040 | sp|Q39096|ERD15_ARATH | 65.000 | 80 | 24 | 2 | 1 | 76 | 1 | 80 | 5.32e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1114040 | Q2HRG8 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 1.40e-67 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000940 | Msa1114040 | 0.842095 | 3.187303e-58 | -8.615850e-47 |
| Msa0008220 | Msa1114040 | 0.806473 | 8.088984e-50 | -8.615850e-47 |
| Msa0008840 | Msa1114040 | 0.812422 | 4.274638e-51 | -8.615850e-47 |
| Msa0014130 | Msa1114040 | 0.806323 | 8.698451e-50 | -8.615850e-47 |
| Msa0015680 | Msa1114040 | 0.829698 | 4.450688e-55 | -8.615850e-47 |
| Msa0019120 | Msa1114040 | 0.801382 | 9.246284e-49 | -8.615850e-47 |
| Msa0025810 | Msa1114040 | 0.815251 | 1.017580e-51 | -8.615850e-47 |
| Msa0026660 | Msa1114040 | 0.813546 | 2.422926e-51 | -8.615850e-47 |
| Msa0030280 | Msa1114040 | 0.821670 | 3.568857e-53 | -8.615850e-47 |
| Msa0033880 | Msa1114040 | 0.826338 | 2.866431e-54 | -8.615850e-47 |
| Msa0039250 | Msa1114040 | 0.807580 | 4.716553e-50 | -8.615850e-47 |
| Msa0041690 | Msa1114040 | 0.809381 | 1.946537e-50 | -8.615850e-47 |
| Msa0055100 | Msa1114040 | 0.803927 | 2.760198e-49 | -8.615850e-47 |
| Msa0057370 | Msa1114040 | 0.821498 | 3.910705e-53 | -8.615850e-47 |
| Msa0057590 | Msa1114040 | 0.818135 | 2.295310e-52 | -8.615850e-47 |
| Msa0057750 | Msa1114040 | 0.802109 | 6.556730e-49 | -8.615850e-47 |
| Msa0061200 | Msa1114040 | 0.810851 | 9.384862e-51 | -8.615850e-47 |
| Msa0065010 | Msa1114040 | 0.812756 | 3.612472e-51 | -8.615850e-47 |
| Msa0070250 | Msa1114040 | 0.807658 | 4.539981e-50 | -8.615850e-47 |
| Msa0077540 | Msa1114040 | 0.808095 | 3.664212e-50 | -8.615850e-47 |
| Msa0100800 | Msa1114040 | 0.857117 | 2.030767e-62 | -8.615850e-47 |
| Msa0101180 | Msa1114040 | 0.816717 | 4.788510e-52 | -8.615850e-47 |
| Msa0108870 | Msa1114040 | 0.832436 | 9.464188e-56 | -8.615850e-47 |
| Msa0115500 | Msa1114040 | 0.815188 | 1.050529e-51 | -8.615850e-47 |
| Msa0118000 | Msa1114040 | 0.801119 | 1.046660e-48 | -8.615850e-47 |
| Msa0610090 | Msa1114040 | 0.811969 | 5.366748e-51 | -8.615850e-47 |
| Msa0615680 | Msa1114040 | 0.807567 | 4.745965e-50 | -8.615850e-47 |
| Msa0619920 | Msa1114040 | 0.807320 | 5.355560e-50 | -8.615850e-47 |
| Msa0626680 | Msa1114040 | 0.818874 | 1.561036e-52 | -8.615850e-47 |
| Msa0640500 | Msa1114040 | 0.817557 | 3.099875e-52 | -8.615850e-47 |
| Msa0658480 | Msa1114040 | 0.813207 | 2.877220e-51 | -8.615850e-47 |
| Msa0659990 | Msa1114040 | 0.814585 | 1.429612e-51 | -8.615850e-47 |
| Msa0664760 | Msa1114040 | 0.813846 | 2.081372e-51 | -8.615850e-47 |
| Msa0679100 | Msa1114040 | 0.814978 | 1.169599e-51 | -8.615850e-47 |
| Msa0689420 | Msa1114040 | 0.804653 | 1.947799e-49 | -8.615850e-47 |
| Msa0707390 | Msa1114040 | 0.803938 | 2.745266e-49 | -8.615850e-47 |
| Msa0716860 | Msa1114040 | 0.811720 | 6.078934e-51 | -8.615850e-47 |
| Msa0720370 | Msa1114040 | 0.804385 | 2.216119e-49 | -8.615850e-47 |
| Msa0742510 | Msa1114040 | 0.817618 | 3.003487e-52 | -8.615850e-47 |
| Msa0758230 | Msa1114040 | 0.805788 | 1.127362e-49 | -8.615850e-47 |
| Msa0776750 | Msa1114040 | 0.811561 | 6.581662e-51 | -8.615850e-47 |
| Msa0779550 | Msa1114040 | 0.810953 | 8.920873e-51 | -8.615850e-47 |
| Msa0795660 | Msa1114040 | 0.810449 | 1.146190e-50 | -8.615850e-47 |
| Msa0813260 | Msa1114040 | 0.800181 | 1.625572e-48 | -8.615850e-47 |
| Msa0814250 | Msa1114040 | 0.803935 | 2.748678e-49 | -8.615850e-47 |
| Msa0818770 | Msa1114040 | 0.837981 | 3.770110e-57 | -8.615850e-47 |
| Msa0819540 | Msa1114040 | 0.812109 | 5.002636e-51 | -8.615850e-47 |
| Msa0822170 | Msa1114040 | 0.801326 | 9.493566e-49 | -8.615850e-47 |
| Msa0824730 | Msa1114040 | 0.800045 | 1.732211e-48 | -8.615850e-47 |
| Msa0125770 | Msa1114040 | 0.801335 | 9.453922e-49 | -8.615850e-47 |
| Msa0126770 | Msa1114040 | 0.806032 | 1.001729e-49 | -8.615850e-47 |
| Msa0127850 | Msa1114040 | 0.810581 | 1.073555e-50 | -8.615850e-47 |
| Msa0133720 | Msa1114040 | 0.800158 | 1.643651e-48 | -8.615850e-47 |
| Msa0134000 | Msa1114040 | 0.806194 | 9.261611e-50 | -8.615850e-47 |
| Msa0140400 | Msa1114040 | 0.810558 | 1.086036e-50 | -8.615850e-47 |
| Msa0145150 | Msa1114040 | 0.824963 | 6.071179e-54 | -8.615850e-47 |
| Msa0165900 | Msa1114040 | 0.804939 | 1.697709e-49 | -8.615850e-47 |
| Msa0171850 | Msa1114040 | 0.805507 | 1.291431e-49 | -8.615850e-47 |
| Msa0174000 | Msa1114040 | 0.835793 | 1.364403e-56 | -8.615850e-47 |
| Msa0177230 | Msa1114040 | 0.819010 | 1.453553e-52 | -8.615850e-47 |
| Msa0181230 | Msa1114040 | 0.806959 | 6.383607e-50 | -8.615850e-47 |
| Msa0193600 | Msa1114040 | 0.806255 | 8.991277e-50 | -8.615850e-47 |
| Msa0198520 | Msa1114040 | 0.801565 | 8.479764e-49 | -8.615850e-47 |
| Msa0198530 | Msa1114040 | 0.802149 | 6.434243e-49 | -8.615850e-47 |
| Msa0202930 | Msa1114040 | 0.800773 | 1.231197e-48 | -8.615850e-47 |
| Msa0208000 | Msa1114040 | 0.809050 | 2.291148e-50 | -8.615850e-47 |
| Msa0208810 | Msa1114040 | 0.810612 | 1.057197e-50 | -8.615850e-47 |
| Msa0212750 | Msa1114040 | -0.833919 | 4.044117e-56 | -8.615850e-47 |
| Msa0221600 | Msa1114040 | 0.851347 | 9.414312e-61 | -8.615850e-47 |
| Msa0236070 | Msa1114040 | 0.804724 | 1.882861e-49 | -8.615850e-47 |
| Msa0384670 | Msa1114040 | 0.832096 | 1.148801e-55 | -8.615850e-47 |
| Msa0384880 | Msa1114040 | 0.829823 | 4.148753e-55 | -8.615850e-47 |
| Msa0392200 | Msa1114040 | 0.809745 | 1.625358e-50 | -8.615850e-47 |
| Msa0394690 | Msa1114040 | 0.826415 | 2.747209e-54 | -8.615850e-47 |
| Msa0403190 | Msa1114040 | 0.807633 | 4.594834e-50 | -8.615850e-47 |
| Msa0434570 | Msa1114040 | 0.818980 | 1.476809e-52 | -8.615850e-47 |
| Msa0434770 | Msa1114040 | 0.832519 | 9.025720e-56 | -8.615850e-47 |
| Msa0436200 | Msa1114040 | 0.825566 | 4.371107e-54 | -8.615850e-47 |
| Msa0439890 | Msa1114040 | 0.808825 | 2.559971e-50 | -8.615850e-47 |
| Msa0442310 | Msa1114040 | 0.801560 | 8.500284e-49 | -8.615850e-47 |
| Msa0444080 | Msa1114040 | 0.826277 | 2.963187e-54 | -8.615850e-47 |
| Msa0461590 | Msa1114040 | 0.811698 | 6.147120e-51 | -8.615850e-47 |
| Msa0474700 | Msa1114040 | 0.811718 | 6.084690e-51 | -8.615850e-47 |
| Msa0478580 | Msa1114040 | 0.834313 | 3.222154e-56 | -8.615850e-47 |
| Msa0981430 | Msa1114040 | 0.811112 | 8.240082e-51 | -8.615850e-47 |
| Msa1010460 | Msa1114040 | 0.827537 | 1.481543e-54 | -8.615850e-47 |
| Msa1030930 | Msa1114040 | 0.801055 | 1.078659e-48 | -8.615850e-47 |
| Msa1036740 | Msa1114040 | 0.800054 | 1.725587e-48 | -8.615850e-47 |
| Msa1038210 | Msa1114040 | -0.816156 | 6.394345e-52 | -8.615850e-47 |
| Msa1070050 | Msa1114040 | 0.810883 | 9.237238e-51 | -8.615850e-47 |
| Msa1071660 | Msa1114040 | 0.858046 | 1.077387e-62 | -8.615850e-47 |
| Msa1073830 | Msa1114040 | 0.838154 | 3.403740e-57 | -8.615850e-47 |
| Msa1076600 | Msa1114040 | 0.816525 | 5.287705e-52 | -8.615850e-47 |
| Msa1083880 | Msa1114040 | 0.828803 | 7.338003e-55 | -8.615850e-47 |
| Msa1089740 | Msa1114040 | -0.801027 | 1.093003e-48 | -8.615850e-47 |
| Msa0244930 | Msa1114040 | 0.804595 | 2.003079e-49 | -8.615850e-47 |
| Msa0252240 | Msa1114040 | 0.824978 | 6.020077e-54 | -8.615850e-47 |
| Msa0265160 | Msa1114040 | 0.804908 | 1.723166e-49 | -8.615850e-47 |
| Msa0273650 | Msa1114040 | 0.805772 | 1.135773e-49 | -8.615850e-47 |
| Msa0274100 | Msa1114040 | 0.822257 | 2.609668e-53 | -8.615850e-47 |
| Msa0291520 | Msa1114040 | 0.817308 | 3.527876e-52 | -8.615850e-47 |
| Msa0346990 | Msa1114040 | 0.808204 | 3.474204e-50 | -8.615850e-47 |
| Msa0363060 | Msa1114040 | 0.801668 | 8.076441e-49 | -8.615850e-47 |
| Msa1113590 | Msa1114040 | 0.849976 | 2.288138e-60 | -8.615850e-47 |
| Msa1114040 | Msa1118710 | 0.844581 | 6.916999e-59 | -8.615850e-47 |
| Msa1114040 | Msa1133630 | 0.842794 | 2.079848e-58 | -8.615850e-47 |
| Msa1114040 | Msa1158770 | 0.868083 | 8.496069e-66 | -8.615850e-47 |
| Msa1114040 | Msa1160000 | 0.864839 | 9.108477e-65 | -8.615850e-47 |
| Msa1114040 | Msa1173900 | 0.829002 | 6.568203e-55 | -8.615850e-47 |
| Msa1114040 | Msa1178370 | 0.801561 | 8.495231e-49 | -8.615850e-47 |
| Msa1114040 | Msa1181170 | -0.838535 | 2.714813e-57 | -8.615850e-47 |
| Msa1114040 | Msa1199290 | 0.806089 | 9.744404e-50 | -8.615850e-47 |
| Msa1114040 | Msa1207730 | 0.809774 | 1.602510e-50 | -8.615850e-47 |
| Msa1114040 | Msa1214320 | 0.801016 | 1.098524e-48 | -8.615850e-47 |
| Msa1114040 | Msa1223330 | 0.834778 | 2.461950e-56 | -8.615850e-47 |
| Msa1114040 | Msa1226610 | 0.813830 | 2.098805e-51 | -8.615850e-47 |
| Msa1114040 | Msa1233950 | 0.818522 | 1.876117e-52 | -8.615850e-47 |
| Msa1114040 | Msa1242640 | 0.810583 | 1.072511e-50 | -8.615850e-47 |
| Msa1114040 | Msa1253810 | 0.809149 | 2.181888e-50 | -8.615850e-47 |
| Msa1114040 | Msa1256770 | 0.813057 | 3.102519e-51 | -8.615850e-47 |
| Msa1114040 | Msa1294960 | 0.840784 | 7.056952e-58 | -8.615850e-47 |
| Msa1114040 | Msa1296300 | 0.808724 | 2.690594e-50 | -8.615850e-47 |
| Msa1114040 | Msa1297800 | 0.837393 | 5.339067e-57 | -8.615850e-47 |
| Msa1114040 | Msa1310690 | 0.809000 | 2.348431e-50 | -8.615850e-47 |
| Msa1114040 | Msa1318620 | 0.834304 | 3.239097e-56 | -8.615850e-47 |
| Msa1114040 | Msa1329450 | 0.864173 | 1.471373e-64 | -8.615850e-47 |
| Msa1114040 | Msa1333970 | 0.828870 | 7.067380e-55 | -8.615850e-47 |
| Msa1114040 | Msa1350440 | 0.801954 | 7.054534e-49 | -8.615850e-47 |
| Msa1114040 | Msa1361250 | 0.802005 | 6.887810e-49 | -8.615850e-47 |
| Msa1114040 | Msa1364060 | 0.803952 | 2.727124e-49 | -8.615850e-47 |
| Msa1114040 | Msa1373820 | 0.807117 | 5.912356e-50 | -8.615850e-47 |
| Msa1114040 | Msa1373880 | 0.842400 | 2.646028e-58 | -8.615850e-47 |
| Msa1114040 | Msa1380970 | 0.800731 | 1.255848e-48 | -8.615850e-47 |
| Msa1114040 | Msa1393800 | 0.804546 | 2.051448e-49 | -8.615850e-47 |
| Msa1114040 | Msa1421900 | 0.818602 | 1.798979e-52 | -8.615850e-47 |
| Msa1114040 | Msa1422840 | 0.829558 | 4.812076e-55 | -8.615850e-47 |
| Msa1114040 | Msa1464290 | 0.802533 | 5.362974e-49 | -8.615850e-47 |
| Msa0527910 | Msa1114040 | 0.810034 | 1.408465e-50 | -8.615850e-47 |
| Msa0533960 | Msa1114040 | -0.802684 | 4.991525e-49 | -8.615850e-47 |
| Msa0534080 | Msa1114040 | 0.821180 | 4.630930e-53 | -8.615850e-47 |
| Msa0537530 | Msa1114040 | 0.820163 | 7.929376e-53 | -8.615850e-47 |
| Msa0546570 | Msa1114040 | 0.802740 | 4.860339e-49 | -8.615850e-47 |
| Msa0558350 | Msa1114040 | 0.824836 | 6.505404e-54 | -8.615850e-47 |
| Msa0576890 | Msa1114040 | 0.835637 | 1.494421e-56 | -8.615850e-47 |
| Msa0578090 | Msa1114040 | 0.837071 | 6.451347e-57 | -8.615850e-47 |
| Msa0583500 | Msa1114040 | 0.839838 | 1.246670e-57 | -8.615850e-47 |
| Msa0586850 | Msa1114040 | 0.829227 | 5.793607e-55 | -8.615850e-47 |
| Msa0597000 | Msa1114040 | 0.806281 | 8.875565e-50 | -8.615850e-47 |
| Msa0599900 | Msa1114040 | 0.812216 | 4.740748e-51 | -8.615850e-47 |
| Msa0869010 | Msa1114040 | 0.812419 | 4.279561e-51 | -8.615850e-47 |
| Msa0895310 | Msa1114040 | 0.819405 | 1.181820e-52 | -8.615850e-47 |
| Msa0914260 | Msa1114040 | 0.836491 | 9.069746e-57 | -8.615850e-47 |
| Msa0921870 | Msa1114040 | 0.802022 | 6.833838e-49 | -8.615850e-47 |
| Msa0928120 | Msa1114040 | 0.802791 | 4.743909e-49 | -8.615850e-47 |
| Msa0947530 | Msa1114040 | -0.806915 | 6.524273e-50 | -8.615850e-47 |
| Msa0955210 | Msa1114040 | 0.807855 | 4.122160e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1114040 | MtrunA17_Chr7g0256851 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 2.69e-71 | 211 |
| Msa1114040 | MtrunA17_Chr3g0085831 | 50.476 | 105 | 48 | 3 | 1 | 102 | 1 | 104 | 3.61e-29 | 105 |
| Msa1114040 | MtrunA17_Chr5g0437861 | 44.872 | 78 | 38 | 2 | 1 | 73 | 1 | 78 | 3.44e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1114040 | AT2G41430.5 | 65.000 | 80 | 24 | 2 | 1 | 76 | 1 | 80 | 5.40e-30 | 107 |
| Msa1114040 | AT2G41430.4 | 65.000 | 80 | 24 | 2 | 1 | 76 | 1 | 80 | 5.40e-30 | 107 |
| Msa1114040 | AT2G41430.2 | 65.000 | 80 | 24 | 2 | 1 | 76 | 1 | 80 | 5.40e-30 | 107 |
| Msa1114040 | AT2G41430.1 | 65.000 | 80 | 24 | 2 | 1 | 76 | 1 | 80 | 5.40e-30 | 107 |
| Msa1114040 | AT2G41430.3 | 65.000 | 80 | 24 | 2 | 1 | 76 | 1 | 80 | 1.02e-29 | 105 |
| Msa1114040 | AT4G14270.1 | 57.407 | 54 | 23 | 0 | 2 | 55 | 3 | 56 | 7.15e-17 | 73.6 |
| Msa1114040 | AT4G14270.2 | 57.407 | 54 | 23 | 0 | 2 | 55 | 3 | 56 | 7.15e-17 | 73.6 |
Find 37 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAATTACCAGAGAAATT+TGG | 0.241148 | 7_3:+60645896 | None:intergenic |
| GTCACCAGCTGCCACCATTC+CGG | 0.246976 | 7_3:+60645272 | None:intergenic |
| TTCAAGATGGCATTAGTTTC+AGG | 0.269984 | 7_3:-60645368 | Msa1114040:exon |
| TCACCAGCTGCCACCATTCC+GGG | 0.312874 | 7_3:+60645273 | None:intergenic |
| AATTCTCTGGCGGCCGAAAA+TGG | 0.315874 | 7_3:-60642739 | Msa1114040:CDS |
| GGAATGTAAAGTGGTGCATT+AGG | 0.338731 | 7_3:+60645326 | None:intergenic |
| GGTTGCATCAACGATGAATA+TGG | 0.391669 | 7_3:+60643508 | None:intergenic |
| TGAAATGGTACCATGATTAC+TGG | 0.394842 | 7_3:-60645244 | Msa1114040:CDS |
| AGCCTTCATCATCATGTTGC+TGG | 0.408811 | 7_3:+60645216 | None:intergenic |
| GAATGTAAAGTGGTGCATTA+GGG | 0.425842 | 7_3:+60645327 | None:intergenic |
| GAAATGTGAAATCATCCATT+TGG | 0.431354 | 7_3:-60643149 | Msa1114040:CDS |
| CTTCAAACTTGACTTCCAAA+TGG | 0.432156 | 7_3:+60643134 | None:intergenic |
| TCTCGCTAACCAAATTTCTC+TGG | 0.450228 | 7_3:-60645905 | Msa1114040:intron |
| TCGACGTAGCGAAATGCAGC+TGG | 0.454423 | 7_3:+60645305 | None:intergenic |
| TTCCCCGGAATGGTGGCAGC+TGG | 0.476894 | 7_3:-60645276 | Msa1114040:CDS |
| CTTAGTTCATCGGGCTCCAT+CGG | 0.496160 | 7_3:+60642711 | None:intergenic |
| GCATCAAGATCAAACGCATC+AGG | 0.498917 | 7_3:+60645143 | None:intergenic |
| ACAACTGCTCTTAGTTCATC+GGG | 0.500188 | 7_3:+60642702 | None:intergenic |
| GCTGGCTAAGCCAGTAATCA+TGG | 0.519480 | 7_3:+60645234 | None:intergenic |
| CGATCCTCTCCCTTCACTAC+CGG | 0.530891 | 7_3:-60643486 | Msa1114040:intron |
| AGCTGGTGACAACACTGAAA+TGG | 0.546239 | 7_3:-60645259 | Msa1114040:CDS |
| GATGATGTAGATGAGTTTGA+CGG | 0.549584 | 7_3:-60645188 | Msa1114040:CDS |
| AGTGGATTCACCGGTAGTGA+AGG | 0.551513 | 7_3:+60643476 | None:intergenic |
| AGCCAGCAACATGATGATGA+AGG | 0.560493 | 7_3:-60645218 | Msa1114040:CDS |
| GCCGAAAATGGTATCACCGA+TGG | 0.562882 | 7_3:-60642727 | Msa1114040:CDS |
| AGCTGCATTTCGCTACGTCG+AGG | 0.567649 | 7_3:-60645303 | Msa1114040:CDS |
| AGAGTTTATCAAGTCTTCTG+AGG | 0.595138 | 7_3:-60645087 | Msa1114040:CDS |
| GTGGATTCACCGGTAGTGAA+GGG | 0.607173 | 7_3:+60643477 | None:intergenic |
| AGAGTGAAGGTGATTCAAGA+TGG | 0.611938 | 7_3:-60645381 | Msa1114040:exon |
| CACCAGCTGCCACCATTCCG+GGG | 0.612813 | 7_3:+60645274 | None:intergenic |
| ATCAACGATGAATATGGTGT+CGG | 0.615997 | 7_3:+60643514 | None:intergenic |
| ATCAAGTCTTCTGAGGCACA+AGG | 0.619098 | 7_3:-60645080 | Msa1114040:intron |
| CGGTAGTGAAGGGAGAGGAT+CGG | 0.625222 | 7_3:+60643487 | None:intergenic |
| AAGATGGCATTAGTTTCAGG+AGG | 0.635369 | 7_3:-60645365 | Msa1114040:CDS |
| TGATGCGTTTGATCTTGATG+CGG | 0.644823 | 7_3:-60645141 | Msa1114040:CDS |
| AATGCAGCTGGAATGTAAAG+TGG | 0.663728 | 7_3:+60645317 | None:intergenic |
| TTCACCGGTAGTGAAGGGAG+AGG | 0.665767 | 7_3:+60643482 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTATGGTTATTTTACAAT+TGG | + | chr7_3:60643023-60643042 | None:intergenic | 15.0% |
| !! | AGTAAGTTTATTATTACAAT+TGG | + | chr7_3:60644153-60644172 | None:intergenic | 15.0% |
| !! | TTTCTTTAATATGACTAATA+AGG | + | chr7_3:60644620-60644639 | None:intergenic | 15.0% |
| !! | GATTCAAATATAAAAAATAG+CGG | - | chr7_3:60644657-60644676 | Msa1114040:intron | 15.0% |
| !! | GAATATAAATAATTGATTGT+CGG | + | chr7_3:60645190-60645209 | None:intergenic | 15.0% |
| !!! | ATTTTAACTGAAAAAACATT+AGG | + | chr7_3:60645297-60645316 | None:intergenic | 15.0% |
| !!! | TGCAATAATTATTTTCAAAT+TGG | + | chr7_3:60645799-60645818 | None:intergenic | 15.0% |
| !!! | CATTGTTAATTGTTGATTAA+TGG | - | chr7_3:60643209-60643228 | Msa1114040:intron | 20.0% |
| !!! | TCTTTTTTTTCATCAACAAA+GGG | + | chr7_3:60644497-60644516 | None:intergenic | 20.0% |
| !!! | ATCTTTTTTTTCATCAACAA+AGG | + | chr7_3:60644498-60644517 | None:intergenic | 20.0% |
| !! | TATAGTTGTTAAGGAAAATA+GGG | + | chr7_3:60644831-60644850 | None:intergenic | 20.0% |
| !! | ATATTAAGCAGAAAAAATAG+TGG | + | chr7_3:60644872-60644891 | None:intergenic | 20.0% |
| !!! | AGCATCTTTCATTTTTTTAT+TGG | + | chr7_3:60644938-60644957 | None:intergenic | 20.0% |
| !! | AATTTGATTGGAAAAACAAA+CGG | + | chr7_3:60645259-60645278 | None:intergenic | 20.0% |
| !! | TTAAAATTCTTCATCTTATC+TGG | - | chr7_3:60645310-60645329 | Msa1114040:CDS | 20.0% |
| !!! | TTTTTAGATGATCATATCAT+AGG | - | chr7_3:60645582-60645601 | Msa1114040:intron | 20.0% |
| !! | AAAAATAAAATAAGGTGAGA+TGG | + | chr7_3:60645833-60645852 | None:intergenic | 20.0% |
| !! | GTGCTGAAAAAAATAAAATA+AGG | + | chr7_3:60645841-60645860 | None:intergenic | 20.0% |
| ! | TGAGTAATTGAAAGAGAAAA+AGG | + | chr7_3:60642762-60642781 | None:intergenic | 25.0% |
| ! | TCATGATGATTCATCAATTA+TGG | + | chr7_3:60643038-60643057 | None:intergenic | 25.0% |
| ! | TTGATTAATGGTGTAGATTA+AGG | - | chr7_3:60643221-60643240 | Msa1114040:intron | 25.0% |
| !! | AACTTTCCTCTGAAAAATAA+CGG | + | chr7_3:60643675-60643694 | None:intergenic | 25.0% |
| !! | CTCTATTTTACCATAGATTT+TGG | + | chr7_3:60643886-60643905 | None:intergenic | 25.0% |
| ! | AAATCTATGGTAAAATAGAG+AGG | - | chr7_3:60643886-60643905 | Msa1114040:intron | 25.0% |
| ! | AGACATGTAAGAAAGTTTAT+GGG | + | chr7_3:60644025-60644044 | None:intergenic | 25.0% |
| ! | AAGACATGTAAGAAAGTTTA+TGG | + | chr7_3:60644026-60644045 | None:intergenic | 25.0% |
| ! | TTTCTTACATGTCTTCAAAA+TGG | - | chr7_3:60644031-60644050 | Msa1114040:intron | 25.0% |
| ! | TTGAAATTTCTATAGTCAAC+CGG | + | chr7_3:60644338-60644357 | None:intergenic | 25.0% |
| !!! | GATATTTTTGTGAATATGAG+AGG | + | chr7_3:60644397-60644416 | None:intergenic | 25.0% |
| ! | AGAAATTAAAACTTGAGAAG+AGG | - | chr7_3:60644635-60644654 | Msa1114040:intron | 25.0% |
| !!! | AATTTTATTTGCTAGGGTTT+AGG | + | chr7_3:60644763-60644782 | None:intergenic | 25.0% |
| !!! | AACAGGAATTTTATTTGCTA+GGG | + | chr7_3:60644769-60644788 | None:intergenic | 25.0% |
| !!! | AAACAGGAATTTTATTTGCT+AGG | + | chr7_3:60644770-60644789 | None:intergenic | 25.0% |
| ! | TTGTTTCGTAATTAAGCATT+AGG | + | chr7_3:60644802-60644821 | None:intergenic | 25.0% |
| ! | GTATAGTTGTTAAGGAAAAT+AGG | + | chr7_3:60644832-60644851 | None:intergenic | 25.0% |
| !! | TGTTTTACGTATAGTTGTTA+AGG | + | chr7_3:60644840-60644859 | None:intergenic | 25.0% |
| ! | TATAAATAATTGATTGTCGG+TGG | + | chr7_3:60645187-60645206 | None:intergenic | 25.0% |
| ! | TTCCAATCAAATTATTGCTT+AGG | - | chr7_3:60645266-60645285 | Msa1114040:CDS | 25.0% |
| ! | AACCTAAGCAATAATTTGAT+TGG | + | chr7_3:60645271-60645290 | None:intergenic | 25.0% |
| !!! | ACAACTAATTGTTTTATGCT+AGG | - | chr7_3:60645643-60645662 | Msa1114040:intron | 25.0% |
| !! | ACAAGTATTTAGTTTACACT+TGG | - | chr7_3:60645741-60645760 | Msa1114040:intron | 25.0% |
| !!! | TTATTTTTTTCAGCACATCA+TGG | - | chr7_3:60645845-60645864 | Msa1114040:intron | 25.0% |
| AAGGAATTACCAGAGAAATT+TGG | + | chr7_3:60642743-60642762 | None:intergenic | 30.0% | |
| !!! | AACATACCGTTATTTTTCAG+AGG | - | chr7_3:60643666-60643685 | Msa1114040:intron | 30.0% |
| !! | TTTTTCAGAGGAAAGTTTTG+TGG | - | chr7_3:60643678-60643697 | Msa1114040:intron | 30.0% |
| ACAGAAAGTTAAAACTGACA+TGG | + | chr7_3:60643817-60643836 | None:intergenic | 30.0% | |
| CTTTCTGTAACTTCTTGTAT+AGG | - | chr7_3:60643829-60643848 | Msa1114040:intron | 30.0% | |
| TTCCTTCTTACCAAAATCTA+TGG | - | chr7_3:60643873-60643892 | Msa1114040:intron | 30.0% | |
| !! | TACCATAGATTTTGGTAAGA+AGG | + | chr7_3:60643878-60643897 | None:intergenic | 30.0% |
| CTAGGGTTTAGGTAAAATTA+GGG | + | chr7_3:60644752-60644771 | None:intergenic | 30.0% | |
| ! | TTGTATTCTGGCTAAAATTG+TGG | + | chr7_3:60644968-60644987 | None:intergenic | 30.0% |
| TGATACGTTTGTTTGTATTC+TGG | + | chr7_3:60644980-60644999 | None:intergenic | 30.0% | |
| TCTAAACTGGATCAAAAAAG+TGG | + | chr7_3:60645076-60645095 | None:intergenic | 30.0% | |
| !!! | CTTTTTTGATCCAGTTTAGA+AGG | - | chr7_3:60645076-60645095 | Msa1114040:intron | 30.0% |
| !! | GTGTTTTTCCTCTTATTTAC+AGG | - | chr7_3:60645460-60645479 | Msa1114040:intron | 30.0% |
| TTTCACAACCTGTAAATAAG+AGG | + | chr7_3:60645471-60645490 | None:intergenic | 30.0% | |
| GAAATGTGAAATCATCCATT+TGG | - | chr7_3:60645487-60645506 | Msa1114040:intron | 30.0% | |
| !!! | TGAAGCTTCTATACTTTTTC+AGG | - | chr7_3:60645520-60645539 | Msa1114040:intron | 30.0% |
| ACCATTAAACTCGATATAGA+GGG | + | chr7_3:60645708-60645727 | None:intergenic | 30.0% | |
| ! | TATTTTCAAATTGGCATCTG+CGG | + | chr7_3:60645790-60645809 | None:intergenic | 30.0% |
| AATAAAATAAGGTGAGATGG+TGG | + | chr7_3:60645830-60645849 | None:intergenic | 30.0% | |
| CAGTAGTAAAAAGAATTCTC+TGG | - | chr7_3:60645884-60645903 | Msa1114040:intron | 30.0% | |
| TAGTAAAAAGAATTCTCTGG+CGG | - | chr7_3:60645887-60645906 | Msa1114040:intron | 30.0% | |
| !! | CAAATCTCTTTTGACTCGTT+TGG | + | chr7_3:60642926-60642945 | None:intergenic | 35.0% |
| GGAAAAAGTGATAAGAGTGA+AGG | - | chr7_3:60643242-60643261 | Msa1114040:intron | 35.0% | |
| ! | TTCAAGATGGCATTAGTTTC+AGG | - | chr7_3:60643268-60643287 | Msa1114040:intron | 35.0% |
| GAATGTAAAGTGGTGCATTA+GGG | + | chr7_3:60643312-60643331 | None:intergenic | 35.0% | |
| TGAAATGGTACCATGATTAC+TGG | - | chr7_3:60643392-60643411 | Msa1114040:intron | 35.0% | |
| GATGATGTAGATGAGTTTGA+CGG | - | chr7_3:60643448-60643467 | Msa1114040:intron | 35.0% | |
| AGAGTTTATCAAGTCTTCTG+AGG | - | chr7_3:60643549-60643568 | Msa1114040:intron | 35.0% | |
| CAAGGAGCAAAGAAATTTAC+TGG | - | chr7_3:60643703-60643722 | Msa1114040:intron | 35.0% | |
| ATTTACTGGATGAGCTAAAG+AGG | - | chr7_3:60643717-60643736 | Msa1114040:intron | 35.0% | |
| AGCTAAATAACCGAAAGCTA+AGG | + | chr7_3:60643788-60643807 | None:intergenic | 35.0% | |
| TGTAACTTCTTGTATAGGCA+AGG | - | chr7_3:60643834-60643853 | Msa1114040:intron | 35.0% | |
| TTCTGGACATGTATCTTGTA+TGG | + | chr7_3:60644118-60644137 | None:intergenic | 35.0% | |
| ATTGGTCAATTGATTGCTTC+TGG | + | chr7_3:60644135-60644154 | None:intergenic | 35.0% | |
| TAGGGCGAAATTATTTGCTT+GGG | + | chr7_3:60644734-60644753 | None:intergenic | 35.0% | |
| TTAGGGCGAAATTATTTGCT+TGG | + | chr7_3:60644735-60644754 | None:intergenic | 35.0% | |
| GCTAGGGTTTAGGTAAAATT+AGG | + | chr7_3:60644753-60644772 | None:intergenic | 35.0% | |
| CGTAATTAAGCATTAGGTCA+GGG | + | chr7_3:60644796-60644815 | None:intergenic | 35.0% | |
| TCGTAATTAAGCATTAGGTC+AGG | + | chr7_3:60644797-60644816 | None:intergenic | 35.0% | |
| !! | GCAGAAAAAATAGTGGATAG+TGG | + | chr7_3:60644865-60644884 | None:intergenic | 35.0% |
| ATCAACGATGAATATGGTGT+CGG | + | chr7_3:60645125-60645144 | None:intergenic | 35.0% | |
| ! | CACTTTTACATACACCTTGT+GGG | + | chr7_3:60645406-60645425 | None:intergenic | 35.0% |
| ! | ACACTTTTACATACACCTTG+TGG | + | chr7_3:60645407-60645426 | None:intergenic | 35.0% |
| CTTCAAACTTGACTTCCAAA+TGG | + | chr7_3:60645505-60645524 | None:intergenic | 35.0% | |
| !!! | CTTCTATACTTTTTCAGGTG+AGG | - | chr7_3:60645525-60645544 | Msa1114040:intron | 35.0% |
| TCCCTCTATATCGAGTTTAA+TGG | - | chr7_3:60645704-60645723 | Msa1114040:intron | 35.0% | |
| CACCATTAAACTCGATATAG+AGG | + | chr7_3:60645709-60645728 | None:intergenic | 35.0% | |
| TCTCGCTAACCAAATTTCTC+TGG | - | chr7_3:60642731-60642750 | Msa1114040:CDS | 40.0% | |
| AGAGTGAAGGTGATTCAAGA+TGG | - | chr7_3:60643255-60643274 | Msa1114040:intron | 40.0% | |
| ! | AAGATGGCATTAGTTTCAGG+AGG | - | chr7_3:60643271-60643290 | Msa1114040:intron | 40.0% |
| GGAATGTAAAGTGGTGCATT+AGG | + | chr7_3:60643313-60643332 | None:intergenic | 40.0% | |
| AATGCAGCTGGAATGTAAAG+TGG | + | chr7_3:60643322-60643341 | None:intergenic | 40.0% | |
| TGATGCGTTTGATCTTGATG+CGG | - | chr7_3:60643495-60643514 | Msa1114040:CDS | 40.0% | |
| CAAAGAGATTTGCACTCACA+TGG | - | chr7_3:60644530-60644549 | Msa1114040:intron | 40.0% | |
| CATTAGGTCAGGGTTAAAAC+AGG | + | chr7_3:60644786-60644805 | None:intergenic | 40.0% | |
| TCTGGCTAAAATTGTGGATC+AGG | + | chr7_3:60644962-60644981 | None:intergenic | 40.0% | |
| CTGGATCAAAAAAGTGGAGA+GGG | + | chr7_3:60645070-60645089 | None:intergenic | 40.0% | |
| ACTGGATCAAAAAAGTGGAG+AGG | + | chr7_3:60645071-60645090 | None:intergenic | 40.0% | |
| GGTTGCATCAACGATGAATA+TGG | + | chr7_3:60645131-60645150 | None:intergenic | 40.0% | |
| GAGAGGAACAAACTTTGAGT+TGG | + | chr7_3:60645212-60645231 | None:intergenic | 40.0% | |
| ACGGAAATGAAGATGAGAGA+TGG | + | chr7_3:60645240-60645259 | None:intergenic | 40.0% | |
| ! | TCCATCGGTGATACCATTTT+CGG | + | chr7_3:60645913-60645932 | None:intergenic | 40.0% |
| ! | GACTCGTTTGGAGTCAAGAT+CGG | + | chr7_3:60642914-60642933 | None:intergenic | 45.0% |
| AGCTGGTGACAACACTGAAA+TGG | - | chr7_3:60643377-60643396 | Msa1114040:intron | 45.0% | |
| ! | AGCCAGCAACATGATGATGA+AGG | - | chr7_3:60643418-60643437 | Msa1114040:intron | 45.0% |
| AGCCTTCATCATCATGTTGC+TGG | + | chr7_3:60643423-60643442 | None:intergenic | 45.0% | |
| GCATCAAGATCAAACGCATC+AGG | + | chr7_3:60643496-60643515 | None:intergenic | 45.0% | |
| !! | ATCAAGTCTTCTGAGGCACA+AGG | - | chr7_3:60643556-60643575 | Msa1114040:intron | 45.0% |
| !! | GAGGAAAGTTTTGTGGAGCA+AGG | - | chr7_3:60643685-60643704 | Msa1114040:intron | 45.0% |
| ! | GATGGTCTTGCCTTAGCTTT+CGG | - | chr7_3:60643775-60643794 | Msa1114040:intron | 45.0% |
| ATGCGATTCCCTTGTGCAAA+GGG | - | chr7_3:60644296-60644315 | Msa1114040:intron | 45.0% | |
| TAGCATGACCCTTTGCACAA+GGG | + | chr7_3:60644307-60644326 | None:intergenic | 45.0% | |
| TTAGCATGACCCTTTGCACA+AGG | + | chr7_3:60644308-60644327 | None:intergenic | 45.0% | |
| TGAATCGCAGAATGAGAGAG+AGG | + | chr7_3:60645025-60645044 | None:intergenic | 45.0% | |
| GTGAGAGATGCCTTCTAAAC+TGG | + | chr7_3:60645089-60645108 | None:intergenic | 45.0% | |
| TAATTGATTGTCGGTGGCAG+TGG | + | chr7_3:60645181-60645200 | None:intergenic | 45.0% | |
| ! | GCTGATTTCTTCAACCCACA+AGG | - | chr7_3:60645389-60645408 | Msa1114040:five_prime_UTR | 45.0% |
| !! | GTTAAAATTAAAATAAAAAT+CGG | + | chr7_3:60642861-60642880 | None:intergenic | 5.0% |
| !!! | TTTTTTAAGTAATAAAAAAT+AGG | + | chr7_3:60644896-60644915 | None:intergenic | 5.0% |
| !!! | TTTATCTTTTTTTTTTTTTT+TGG | - | chr7_3:60645607-60645626 | Msa1114040:intron | 5.0% |
| ! | CTACGTCGAGGATTTTTCCC+CGG | - | chr7_3:60643345-60643364 | Msa1114040:intron | 50.0% |
| ! | TCGAGGATTTTTCCCCGGAA+TGG | - | chr7_3:60643350-60643369 | Msa1114040:intron | 50.0% |
| ! | AGGATTTTTCCCCGGAATGG+TGG | - | chr7_3:60643353-60643372 | Msa1114040:intron | 50.0% |
| GCTGGCTAAGCCAGTAATCA+TGG | + | chr7_3:60643405-60643424 | None:intergenic | 50.0% | |
| ACCATAGACGCTGAATGCGA+TGG | - | chr7_3:60643757-60643776 | Msa1114040:intron | 50.0% | |
| ACCATCGCATTCAGCGTCTA+TGG | + | chr7_3:60643761-60643780 | None:intergenic | 50.0% | |
| GATGCGATTCCCTTGTGCAA+AGG | - | chr7_3:60644295-60644314 | Msa1114040:intron | 50.0% | |
| AGAATGAGAGAGAGGAGGAG+GGG | + | chr7_3:60645017-60645036 | None:intergenic | 50.0% | |
| CAGAATGAGAGAGAGGAGGA+GGG | + | chr7_3:60645018-60645037 | None:intergenic | 50.0% | |
| ATCGCAGAATGAGAGAGAGG+AGG | + | chr7_3:60645022-60645041 | None:intergenic | 50.0% | |
| GAGGGATGTGAAACTCTGAG+AGG | + | chr7_3:60645052-60645071 | None:intergenic | 50.0% | |
| GTGGATTCACCGGTAGTGAA+GGG | + | chr7_3:60645162-60645181 | None:intergenic | 50.0% | |
| ! | AGTGGATTCACCGGTAGTGA+AGG | + | chr7_3:60645163-60645182 | None:intergenic | 50.0% |
| GATGAGAGATGGAGAGAGAG+AGG | + | chr7_3:60645229-60645248 | None:intergenic | 50.0% | |
| AATTCTCTGGCGGCCGAAAA+TGG | - | chr7_3:60645897-60645916 | Msa1114040:intron | 50.0% | |
| GCCGAAAATGGTATCACCGA+TGG | - | chr7_3:60645909-60645928 | Msa1114040:five_prime_UTR | 50.0% | |
| TCGACGTAGCGAAATGCAGC+TGG | + | chr7_3:60643334-60643353 | None:intergenic | 55.0% | |
| AGCTGCATTTCGCTACGTCG+AGG | - | chr7_3:60643333-60643352 | Msa1114040:intron | 55.0% | |
| GGGTCATGCTAACTAGCGTC+CGG | - | chr7_3:60644316-60644335 | Msa1114040:intron | 55.0% | |
| GCAGAATGAGAGAGAGGAGG+AGG | + | chr7_3:60645019-60645038 | None:intergenic | 55.0% | |
| CGGTAGTGAAGGGAGAGGAT+CGG | + | chr7_3:60645152-60645171 | None:intergenic | 55.0% | |
| CGATCCTCTCCCTTCACTAC+CGG | - | chr7_3:60645150-60645169 | Msa1114040:CDS | 55.0% | |
| TTCACCGGTAGTGAAGGGAG+AGG | + | chr7_3:60645157-60645176 | None:intergenic | 55.0% | |
| TCACCAGCTGCCACCATTCC+GGG | + | chr7_3:60643366-60643385 | None:intergenic | 60.0% | |
| GTCACCAGCTGCCACCATTC+CGG | + | chr7_3:60643367-60643386 | None:intergenic | 60.0% | |
| GAGAGAGAGGAGGAGGGGAA+GGG | + | chr7_3:60645012-60645031 | None:intergenic | 60.0% | |
| TGAGAGAGAGGAGGAGGGGA+AGG | + | chr7_3:60645013-60645032 | None:intergenic | 60.0% | |
| ! | GTCGGTGGCAGTGGATTCAC+CGG | + | chr7_3:60645172-60645191 | None:intergenic | 60.0% |
| TTCCCCGGAATGGTGGCAGC+TGG | - | chr7_3:60643360-60643379 | Msa1114040:intron | 65.0% | |
| CACCAGCTGCCACCATTCCG+GGG | + | chr7_3:60643365-60643384 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 60642717 | 60645941 | 60642717 | ID=Msa1114040;Name=Msa1114040 |
| chr7_3 | mRNA | 60642717 | 60645941 | 60642717 | ID=Msa1114040-mRNA-1;Parent=Msa1114040;Name=Msa1114040-mRNA-1;_AED=0.47;_eAED=0.47;_QI=73|0.25|0.4|0.4|0.75|0.6|5|0|174 |
| chr7_3 | exon | 60642717 | 60642800 | 60642717 | ID=Msa1114040-mRNA-1:exon:13707;Parent=Msa1114040-mRNA-1 |
| chr7_3 | exon | 60643117 | 60643176 | 60643117 | ID=Msa1114040-mRNA-1:exon:13706;Parent=Msa1114040-mRNA-1 |
| chr7_3 | exon | 60643487 | 60643563 | 60643487 | ID=Msa1114040-mRNA-1:exon:13705;Parent=Msa1114040-mRNA-1 |
| chr7_3 | exon | 60645081 | 60645421 | 60645081 | ID=Msa1114040-mRNA-1:exon:13704;Parent=Msa1114040-mRNA-1 |
| chr7_3 | exon | 60645906 | 60645941 | 60645906 | ID=Msa1114040-mRNA-1:exon:13703;Parent=Msa1114040-mRNA-1 |
| chr7_3 | five_prime_UTR | 60645906 | 60645941 | 60645906 | ID=Msa1114040-mRNA-1:five_prime_utr;Parent=Msa1114040-mRNA-1 |
| chr7_3 | five_prime_UTR | 60645385 | 60645421 | 60645385 | ID=Msa1114040-mRNA-1:five_prime_utr;Parent=Msa1114040-mRNA-1 |
| chr7_3 | CDS | 60645081 | 60645384 | 60645081 | ID=Msa1114040-mRNA-1:cds;Parent=Msa1114040-mRNA-1 |
| chr7_3 | CDS | 60643487 | 60643563 | 60643487 | ID=Msa1114040-mRNA-1:cds;Parent=Msa1114040-mRNA-1 |
| chr7_3 | CDS | 60643117 | 60643176 | 60643117 | ID=Msa1114040-mRNA-1:cds;Parent=Msa1114040-mRNA-1 |
| chr7_3 | CDS | 60642717 | 60642800 | 60642717 | ID=Msa1114040-mRNA-1:cds;Parent=Msa1114040-mRNA-1 |
| Gene Sequence |
| Protein sequence |
