AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1160410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1160410 MtrunA17_Chr5g0413701 94.828 58 3 0 28 85 75 132 4.55e-35 121
Msa1160410 MtrunA17_Chr8g0365071 89.655 58 6 0 28 85 74 131 2.33e-32 114
Msa1160410 MtrunA17_Chr3g0117881 74.576 59 15 0 27 85 63 121 1.92e-29 106
Msa1160410 MtrunA17_Chr3g0126551 80.000 55 11 0 29 83 90 144 1.63e-26 99.4
Msa1160410 MtrunA17_Chr1g0158851 74.545 55 14 0 29 83 95 149 9.20e-25 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1160410 AT1G23820.1 94.828 58 3 0 28 85 67 124 4.89e-35 121
Msa1160410 AT1G23820.2 94.828 58 3 0 28 85 111 168 1.46e-34 120
Msa1160410 AT1G70310.1 93.103 58 4 0 28 85 71 128 1.09e-33 118
Msa1160410 AT5G53120.6 75.862 58 14 0 28 85 90 147 2.94e-27 102
Msa1160410 AT5G53120.1 76.786 56 13 0 28 83 90 145 7.95e-27 100
Msa1160410 AT5G53120.4 76.786 56 13 0 28 83 90 145 7.95e-27 100
Msa1160410 AT5G53120.2 76.786 56 13 0 28 83 90 145 7.95e-27 100
Msa1160410 AT5G53120.5 76.786 56 13 0 28 83 90 145 7.95e-27 100
Msa1160410 AT5G53120.3 76.786 56 13 0 28 83 90 145 7.95e-27 100
Msa1160410 AT5G53120.7 76.786 56 13 0 28 83 107 162 9.70e-27 100
Msa1160410 AT5G19530.2 45.763 59 32 0 27 85 45 103 3.99e-11 57.4
Msa1160410 AT5G19530.1 45.763 59 32 0 27 85 52 110 4.00e-11 57.4

Find 105 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTATTTACAATGAATTT+TGG 0.165714 7_4:-50301440 None:intergenic
ACCCTAGATTTACTTGTTAA+TGG 0.166237 7_4:-50300115 None:intergenic
TTTCATAAATTCTGCATAAT+TGG 0.176833 7_4:-50302171 None:intergenic
AGAACACTAGTTTAACTTTA+AGG 0.198585 7_4:+50301570 Msa1160410:three_prime_UTR
GCTTTCGAGTTTGTGTTTAT+TGG 0.221634 7_4:+50302970 Msa1160410:three_prime_UTR
TTGAAGGGAGTGACTTTAAT+TGG 0.239443 7_4:+50302766 Msa1160410:three_prime_UTR
GAATCTAAAGTGAAATTCTT+AGG 0.243202 7_4:-50302583 None:intergenic
ATATATTGAATTGTATTATT+AGG 0.249029 7_4:+50302139 Msa1160410:three_prime_UTR
GATAAGACTTTGCAAATTCT+AGG 0.257756 7_4:+50303184 Msa1160410:three_prime_UTR
TAGGCTCACCAGATTTAAAA+AGG 0.271869 7_4:+50299796 None:intergenic
TCAAGGAAAATCTGATTATC+AGG 0.273203 7_4:+50300314 Msa1160410:CDS
ATTCTAGACTCTCGTCTAAT+TGG 0.277216 7_4:-50301936 None:intergenic
ATATGGCAAGGTTCTTGTTT+TGG 0.280455 7_4:+50300475 Msa1160410:CDS
CTCATCCTGCTTGATGCTTT+GGG 0.284433 7_4:+50299992 Msa1160410:five_prime_UTR
CTCAATTCCAAACCCCAAAA+AGG 0.285078 7_4:+50300568 Msa1160410:CDS
TACTATATAAGTTGCTCATA+TGG 0.288773 7_4:+50301415 Msa1160410:three_prime_UTR
TCTATATTTAAGTTTCAAAT+AGG 0.290705 7_4:+50302290 Msa1160410:three_prime_UTR
CCTCATCCTGCTTGATGCTT+TGG 0.294134 7_4:+50299991 Msa1160410:five_prime_UTR
ACTATTTGCTGTGGGTTATC+TGG 0.301403 7_4:-50301858 None:intergenic
TGAGCAACTTATATAGTAAA+AGG 0.305909 7_4:-50301410 None:intergenic
GCTGGAAGTTTAATTGAATC+AGG 0.311709 7_4:+50301977 Msa1160410:three_prime_UTR
TTAAGAGATTCCTTGCATTA+AGG 0.326915 7_4:+50302629 Msa1160410:three_prime_UTR
CTCAACTAAACAAACAAATA+AGG 0.327016 7_4:+50302062 Msa1160410:three_prime_UTR
GTCAAATTACAAAATAATTC+TGG 0.327460 7_4:-50303327 None:intergenic
ATTGAAGATATATATTGATT+TGG 0.332478 7_4:-50302263 None:intergenic
TCAGGATGTTATGGTCTTCC+AGG 0.350984 7_4:+50300332 Msa1160410:CDS
GCTAGTGAGTGTAAACCTTA+TGG 0.357006 7_4:-50301759 None:intergenic
ACCTATTTGAAGTTTCAAAT+AGG 0.360092 7_4:-50302332 None:intergenic
TCTCATCTCTATGCAATTTG+TGG 0.365316 7_4:+50300024 Msa1160410:five_prime_UTR
AGCCATGTTGCCAGATGAAA+TGG 0.371483 7_4:+50303129 Msa1160410:intron
AGCTAATAGGAGGCATCTCC+AGG 0.373722 7_4:-50302491 None:intergenic
ATCTGATTATCAGGATGTTA+TGG 0.375563 7_4:+50300323 Msa1160410:CDS
TAAAAGAAGACATGTAAGTA+TGG 0.376983 7_4:+50301607 Msa1160410:three_prime_UTR
TGGCTATTGATCACTACTAT+TGG 0.380310 7_4:-50302114 None:intergenic
TAATACAATTCAATATATAA+TGG 0.380790 7_4:-50302134 None:intergenic
TTGCCAAGGCCCATTTCATC+TGG 0.381270 7_4:-50303139 None:intergenic
TCCTTGCATTAAGGCCTTAG+TGG 0.387826 7_4:+50302638 Msa1160410:three_prime_UTR
AGGTTATGGTGCTGCATCAT+TGG 0.392031 7_4:+50302907 Msa1160410:intron
AGCTGAAATGTGTGTGGGTT+TGG 0.394879 7_4:-50299868 None:intergenic
CATATAAAAGAGAAAGATAA+TGG 0.400480 7_4:+50301909 Msa1160410:three_prime_UTR
TATAGATGATGAAAGATTGC+TGG 0.400963 7_4:+50301959 Msa1160410:three_prime_UTR
TCTGTTTGTGTTCTGTTCCA+TGG 0.406777 7_4:+50302606 Msa1160410:three_prime_UTR
ACCTATTTGAAACTTCAAAT+AGG 0.409996 7_4:+50302331 Msa1160410:three_prime_UTR
CGATTCAAAACATGATTTGA+AGG 0.410794 7_4:+50302715 Msa1160410:three_prime_UTR
ATTGTGCAGAAGTTGTATAT+TGG 0.412189 7_4:-50301813 None:intergenic
AATCTAAAGTGAAATTCTTA+GGG 0.412768 7_4:-50302582 None:intergenic
ACATTATTTATTGTAGCTCC+TGG 0.422852 7_4:-50301494 None:intergenic
TGGTTCAATTTATTGTTTCA+AGG 0.425543 7_4:+50300297 Msa1160410:CDS
TGTAGGATAATGTGGTTTGA+AGG 0.427691 7_4:+50302750 Msa1160410:three_prime_UTR
CTCGTAAAGAATCAATGAAA+TGG 0.432007 7_4:-50302407 None:intergenic
ATGAGTGATCATCTCTTGAT+AGG 0.439438 7_4:-50300534 None:intergenic
GAAGGCTGTCTTTGTTATGT+AGG 0.448540 7_4:+50302733 Msa1160410:three_prime_UTR
GCGGCGCGGCTTGGTGGTGA+TGG 0.463706 7_4:-50299929 None:intergenic
TAATTCTGGACCACAATGTA+TGG 0.468481 7_4:-50303313 None:intergenic
ATCAAGCAGGATGAGGATCT+AGG 0.479107 7_4:-50299984 None:intergenic
TATAATCATTATCTGTGAAA+AGG 0.487173 7_4:-50302229 None:intergenic
AGATGTTGTGCGGCGCGGCT+TGG 0.491448 7_4:-50299938 None:intergenic
TGGAGATGCCTCCTATTAGC+TGG 0.499212 7_4:+50302493 Msa1160410:three_prime_UTR
CAGGATGAGGATCTAGGAGT+TGG 0.500206 7_4:-50299978 None:intergenic
CTTTGTTATGTAGGATAATG+TGG 0.501662 7_4:+50302742 Msa1160410:three_prime_UTR
ATCATATCAACTATTTGCTG+TGG 0.505981 7_4:-50301867 None:intergenic
AGGATCTAGGAGTTGGAAGG+TGG 0.509359 7_4:-50299971 None:intergenic
CATCATTGGATTCATTGTTG+TGG 0.512288 7_4:+50302921 Msa1160410:three_prime_UTR
TTGCCAGATGAAATGGGCCT+TGG 0.512607 7_4:+50303136 Msa1160410:three_prime_UTR
GGATTAACACAGTGCCACTA+AGG 0.521088 7_4:-50302652 None:intergenic
GTCAGCAAAGCAGAACAACT+TGG 0.521268 7_4:-50302207 None:intergenic
TGTTGTGCGGCGCGGCTTGG+TGG 0.523847 7_4:-50299935 None:intergenic
TGAGGTATGATCGCAAGTAC+TGG 0.531212 7_4:+50301524 Msa1160410:three_prime_UTR
GTAGGATAATGTGGTTTGAA+GGG 0.532173 7_4:+50302751 Msa1160410:three_prime_UTR
GGTCATTCAGCTCACAGAAT+GGG 0.533140 7_4:+50300502 Msa1160410:CDS
CAGAACAACTTGGAAGGTCC+TGG 0.551412 7_4:-50302197 None:intergenic
TCATTGGACCAGCTAATAGG+AGG 0.558721 7_4:-50302501 None:intergenic
GGTGACAGATTCATATAGAT+CGG 0.559619 7_4:-50300094 None:intergenic
GGGTCATTCAGCTCACAGAA+TGG 0.563052 7_4:+50300501 Msa1160410:CDS
GCCCATTTCATCTGGCAACA+TGG 0.565590 7_4:-50303131 None:intergenic
GCCACTAAGGCCTTAATGCA+AGG 0.569540 7_4:-50302639 None:intergenic
CCAAAGCATCAAGCAGGATG+AGG 0.571256 7_4:-50299991 None:intergenic
CAGGTGATCATATATATGCT+AGG 0.571290 7_4:-50301718 None:intergenic
GCGCGGCTTGGTGGTGATGG+AGG 0.572676 7_4:-50299926 None:intergenic
TCAGTCAGCAACATATGGCA+AGG 0.575511 7_4:+50300463 Msa1160410:intron
GGACTAAACTAGTGGAGACC+AGG 0.575942 7_4:+50301476 Msa1160410:three_prime_UTR
AGATGAAATGGGCCTTGGCA+AGG 0.578318 7_4:+50303141 Msa1160410:three_prime_UTR
TCATATCAACTATTTGCTGT+GGG 0.579703 7_4:-50301866 None:intergenic
ATTGCAAGAATATCACACTA+AGG 0.585195 7_4:+50302790 Msa1160410:three_prime_UTR
ATAAATAATGTTATTACATG+AGG 0.587172 7_4:+50301506 Msa1160410:three_prime_UTR
GCCATGTTGCCAGATGAAAT+GGG 0.590277 7_4:+50303130 Msa1160410:intron
GCAGAATTTATGAAAGTACC+AGG 0.595328 7_4:+50302179 Msa1160410:three_prime_UTR
TGCAAGGAATCTCTTAACCA+TGG 0.598270 7_4:-50302623 None:intergenic
CAGTAAGATGTTGTGCGGCG+CGG 0.608853 7_4:-50299943 None:intergenic
TGAAAGAAGGACTAAACTAG+TGG 0.611120 7_4:+50301468 Msa1160410:three_prime_UTR
AATAAACGATTTCAAGACTG+TGG 0.614793 7_4:+50301675 Msa1160410:three_prime_UTR
GCAAAGCAGAACAACTTGGA+AGG 0.618468 7_4:-50302203 None:intergenic
CAACACCCAAAGCATCAAGC+AGG 0.618671 7_4:-50299997 None:intergenic
AATAAGCTATTAACATGACA+TGG 0.619044 7_4:-50303246 None:intergenic
ATGAGGATCTAGGAGTTGGA+AGG 0.632854 7_4:-50299974 None:intergenic
GGGTTTGGAATTGAGCAAAG+AGG 0.635906 7_4:-50300560 None:intergenic
GTTTACATGACCATACATTG+TGG 0.639084 7_4:+50303303 Msa1160410:three_prime_UTR
TATATGCTAGGAAAATGCAA+TGG 0.645032 7_4:-50301706 None:intergenic
AATTTGCAAAGTCTTATCAA+CGG 0.647874 7_4:-50303179 None:intergenic
ATCTAAAGTGAAATTCTTAG+GGG 0.656161 7_4:-50302581 None:intergenic
TAAATATGGATATTGTACCC+TGG 0.658840 7_4:+50302473 Msa1160410:three_prime_UTR
TGAAATGGGCCTTGGCAAGG+AGG 0.663901 7_4:+50303144 Msa1160410:three_prime_UTR
TCGCGAGCTGAAATGTGTGT+GGG 0.671216 7_4:-50299873 None:intergenic
GCTAATAGGAGGCATCTCCA+GGG 0.677191 7_4:-50302490 None:intergenic
AATCGCAGTAAGATGTTGTG+CGG 0.683312 7_4:-50299948 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAACTAATTTTTTTTGT+AGG - chr7_4:50301281-50301300 None:intergenic 10.0%
!! TAATACAATTCAATATATAA+TGG - chr7_4:50302137-50302156 None:intergenic 10.0%
!! ATATATTGAATTGTATTATT+AGG + chr7_4:50302139-50302158 Msa1160410:three_prime_UTR 10.0%
!! ATTGAAAAAAATAAATAACA+TGG - chr7_4:50302676-50302695 None:intergenic 10.0%
!! ATTATACCTAAAATTAATGA+TGG - chr7_4:50300277-50300296 None:intergenic 15.0%
!!! TCATTAATTTTAGGTATAAT+TGG + chr7_4:50300277-50300296 Msa1160410:intron 15.0%
!! ACATATACTAAATTATTAGA+GGG - chr7_4:50300611-50300630 None:intergenic 15.0%
!! ATAAATAATGTTATTACATG+AGG + chr7_4:50301506-50301525 Msa1160410:three_prime_UTR 15.0%
!!! ATTGAAGATATATATTGATT+TGG - chr7_4:50302266-50302285 None:intergenic 15.0%
!!! TCTATATTTAAGTTTCAAAT+AGG + chr7_4:50302290-50302309 Msa1160410:three_prime_UTR 15.0%
!!! AAAGTATCAAATACCTTTTT+GGG - chr7_4:50300584-50300603 None:intergenic 20.0%
!! GACATATACTAAATTATTAG+AGG - chr7_4:50300612-50300631 None:intergenic 20.0%
!! AATTCAGGATAATAATTGTA+AGG + chr7_4:50300672-50300691 Msa1160410:intron 20.0%
!!! TTTTAATCATTACATGTAGA+TGG + chr7_4:50300706-50300725 Msa1160410:intron 20.0%
!! TTTGTTACCAACAAAATAAA+GGG - chr7_4:50301049-50301068 None:intergenic 20.0%
!!! ATTTGTTACCAACAAAATAA+AGG - chr7_4:50301050-50301069 None:intergenic 20.0%
!! TGCTAGATTTAAATTAGTAA+GGG - chr7_4:50301254-50301273 None:intergenic 20.0%
!! TTGCTAGATTTAAATTAGTA+AGG - chr7_4:50301255-50301274 None:intergenic 20.0%
!!! TAGCTATTTACAATGAATTT+TGG - chr7_4:50301443-50301462 None:intergenic 20.0%
!!! AATAGCTATTTTATGAAAGA+AGG + chr7_4:50301455-50301474 Msa1160410:three_prime_UTR 20.0%
!! CATATAAAAGAGAAAGATAA+TGG + chr7_4:50301909-50301928 Msa1160410:three_prime_UTR 20.0%
!! TTTCATAAATTCTGCATAAT+TGG - chr7_4:50302174-50302193 None:intergenic 20.0%
!! TATAATCATTATCTGTGAAA+AGG - chr7_4:50302232-50302251 None:intergenic 20.0%
!!! TATTTACTGCTAAACTTTTT+AGG - chr7_4:50302459-50302478 None:intergenic 20.0%
!! AAAAAGTTTAGCAGTAAATA+TGG + chr7_4:50302459-50302478 Msa1160410:three_prime_UTR 20.0%
!!! AATCTAAAGTGAAATTCTTA+GGG - chr7_4:50302585-50302604 None:intergenic 20.0%
!! GTCAAATTACAAAATAATTC+TGG - chr7_4:50303330-50303349 None:intergenic 20.0%
! ACCATTAACAAGTAAATCTA+GGG + chr7_4:50300114-50300133 Msa1160410:intron 25.0%
!! CTTTCTCCATCATTAATTTT+AGG + chr7_4:50300268-50300287 Msa1160410:intron 25.0%
! TGGTTCAATTTATTGTTTCA+AGG + chr7_4:50300297-50300316 Msa1160410:CDS 25.0%
!! AAGTATCAAATACCTTTTTG+GGG - chr7_4:50300583-50300602 None:intergenic 25.0%
!!! CAAAGTATCAAATACCTTTT+TGG - chr7_4:50300585-50300604 None:intergenic 25.0%
! AGAAAATCAATGACAATAAC+TGG - chr7_4:50300634-50300653 None:intergenic 25.0%
!!! ATTGATTTTCTCATGTAGTT+TGG + chr7_4:50300643-50300662 Msa1160410:intron 25.0%
! AATGTCTATATTGAATCTCT+AGG + chr7_4:50300753-50300772 Msa1160410:intron 25.0%
! TTAATGGGATTTGAACATAA+TGG + chr7_4:50300856-50300875 Msa1160410:intron 25.0%
! TAATGGGATTTGAACATAAT+GGG + chr7_4:50300857-50300876 Msa1160410:intron 25.0%
!!! ATAGAGTTTTAAGAAGATGA+TGG - chr7_4:50300970-50300989 None:intergenic 25.0%
!!! TTTCATACCCTTTATTTTGT+TGG + chr7_4:50301039-50301058 Msa1160410:intron 25.0%
! AAACATATATAATGAAGTGC+AGG + chr7_4:50301189-50301208 Msa1160410:intron 25.0%
! AACATATATAATGAAGTGCA+GGG + chr7_4:50301190-50301209 Msa1160410:intron 25.0%
! TGAGCAACTTATATAGTAAA+AGG - chr7_4:50301413-50301432 None:intergenic 25.0%
! TACTATATAAGTTGCTCATA+TGG + chr7_4:50301415-50301434 Msa1160410:three_prime_UTR 25.0%
!! AGAACACTAGTTTAACTTTA+AGG + chr7_4:50301570-50301589 Msa1160410:three_prime_UTR 25.0%
! TAAAAGAAGACATGTAAGTA+TGG + chr7_4:50301607-50301626 Msa1160410:three_prime_UTR 25.0%
! CTCAACTAAACAAACAAATA+AGG + chr7_4:50302062-50302081 Msa1160410:three_prime_UTR 25.0%
!! TGATTTTGCTGCTATAAATT+TGG - chr7_4:50302090-50302109 None:intergenic 25.0%
!! ACCTATTTGAAACTTCAAAT+AGG + chr7_4:50302331-50302350 Msa1160410:three_prime_UTR 25.0%
!! ACCTATTTGAAGTTTCAAAT+AGG - chr7_4:50302335-50302354 None:intergenic 25.0%
! GTCCAATGAAAAATATTACT+TGG + chr7_4:50302515-50302534 Msa1160410:three_prime_UTR 25.0%
!! CTCCAAGTAATATTTTTCAT+TGG - chr7_4:50302520-50302539 None:intergenic 25.0%
!! ATCTAAAGTGAAATTCTTAG+GGG - chr7_4:50302584-50302603 None:intergenic 25.0%
!! GAATCTAAAGTGAAATTCTT+AGG - chr7_4:50302586-50302605 None:intergenic 25.0%
! ATTATGGATTTCTCAATCTT+AGG + chr7_4:50302876-50302895 Msa1160410:intron 25.0%
!! AATTTGCAAAGTCTTATCAA+CGG - chr7_4:50303182-50303201 None:intergenic 25.0%
! AATAAGCTATTAACATGACA+TGG - chr7_4:50303249-50303268 None:intergenic 25.0%
CACCATTAACAAGTAAATCT+AGG + chr7_4:50300113-50300132 Msa1160410:intron 30.0%
! ACCCTAGATTTACTTGTTAA+TGG - chr7_4:50300118-50300137 None:intergenic 30.0%
TGATTCGTATTGTTGTATCT+AGG + chr7_4:50300141-50300160 Msa1160410:intron 30.0%
GATTCGTATTGTTGTATCTA+GGG + chr7_4:50300142-50300161 Msa1160410:intron 30.0%
TCAAGGAAAATCTGATTATC+AGG + chr7_4:50300314-50300333 Msa1160410:CDS 30.0%
ATCTGATTATCAGGATGTTA+TGG + chr7_4:50300323-50300342 Msa1160410:CDS 30.0%
GTAGTTTGGCAAAAAAATTC+AGG + chr7_4:50300657-50300676 Msa1160410:intron 30.0%
!! TCATAGCAACACTGTTTTAA+TGG + chr7_4:50300840-50300859 Msa1160410:intron 30.0%
!!! CATAGCAACACTGTTTTAAT+GGG + chr7_4:50300841-50300860 Msa1160410:intron 30.0%
!! ACATAATGGGTTACAGTTAA+TGG + chr7_4:50300870-50300889 Msa1160410:intron 30.0%
!! GTTACAGTTAATGGATTTGA+GGG + chr7_4:50300879-50300898 Msa1160410:intron 30.0%
ACTAAAGTATGTGTTAGTCA+TGG - chr7_4:50301163-50301182 None:intergenic 30.0%
GTGAATAAGAGTGATATTGT+AGG + chr7_4:50301347-50301366 Msa1160410:intron 30.0%
!!! TGATGACACTTTTTCTATGA+TGG - chr7_4:50301387-50301406 None:intergenic 30.0%
ACATTATTTATTGTAGCTCC+TGG - chr7_4:50301497-50301516 None:intergenic 30.0%
AATAAACGATTTCAAGACTG+TGG + chr7_4:50301675-50301694 Msa1160410:three_prime_UTR 30.0%
TATATGCTAGGAAAATGCAA+TGG - chr7_4:50301709-50301728 None:intergenic 30.0%
! TTTTTAGATAGCACTCCATA+AGG + chr7_4:50301744-50301763 Msa1160410:three_prime_UTR 30.0%
ATTGTGCAGAAGTTGTATAT+TGG - chr7_4:50301816-50301835 None:intergenic 30.0%
!! TCATATCAACTATTTGCTGT+GGG - chr7_4:50301869-50301888 None:intergenic 30.0%
! ATCATATCAACTATTTGCTG+TGG - chr7_4:50301870-50301889 None:intergenic 30.0%
TATAGATGATGAAAGATTGC+TGG + chr7_4:50301959-50301978 Msa1160410:three_prime_UTR 30.0%
!! CTCAAAATGGTTCACTTTTA+AGG - chr7_4:50302358-50302377 None:intergenic 30.0%
! CTTAAAAGTGAACCATTTTG+AGG + chr7_4:50302356-50302375 Msa1160410:three_prime_UTR 30.0%
CTCGTAAAGAATCAATGAAA+TGG - chr7_4:50302410-50302429 None:intergenic 30.0%
TAAATATGGATATTGTACCC+TGG + chr7_4:50302473-50302492 Msa1160410:three_prime_UTR 30.0%
TGAAAAATATTACTTGGAGC+AGG + chr7_4:50302521-50302540 Msa1160410:three_prime_UTR 30.0%
TTAAGAGATTCCTTGCATTA+AGG + chr7_4:50302629-50302648 Msa1160410:three_prime_UTR 30.0%
CGATTCAAAACATGATTTGA+AGG + chr7_4:50302715-50302734 Msa1160410:three_prime_UTR 30.0%
! CTTTGTTATGTAGGATAATG+TGG + chr7_4:50302742-50302761 Msa1160410:three_prime_UTR 30.0%
ATTGCAAGAATATCACACTA+AGG + chr7_4:50302790-50302809 Msa1160410:three_prime_UTR 30.0%
AATCCATAATGAAAACACAG+TGG - chr7_4:50302866-50302885 None:intergenic 30.0%
!!! GCAATTTTTTTGAAGCTTTG+TGG + chr7_4:50303019-50303038 Msa1160410:three_prime_UTR 30.0%
!!! AAGCTTTGTGGTTTTGATAA+TGG + chr7_4:50303031-50303050 Msa1160410:three_prime_UTR 30.0%
TTGTGCTTTAGTGTTTACTA+TGG + chr7_4:50303062-50303081 Msa1160410:intron 30.0%
GATAAGACTTTGCAAATTCT+AGG + chr7_4:50303184-50303203 Msa1160410:three_prime_UTR 30.0%
TCTCATCTCTATGCAATTTG+TGG + chr7_4:50300024-50300043 Msa1160410:five_prime_UTR 35.0%
GGTGACAGATTCATATAGAT+CGG - chr7_4:50300097-50300116 None:intergenic 35.0%
GCATCTAAGAATGAAACTGA+CGG - chr7_4:50300177-50300196 None:intergenic 35.0%
AACAGAACTAGGACTATGAA+GGG - chr7_4:50300393-50300412 None:intergenic 35.0%
AAACAGAACTAGGACTATGA+AGG - chr7_4:50300394-50300413 None:intergenic 35.0%
CATATGTCGTAAACAGAACT+AGG - chr7_4:50300404-50300423 None:intergenic 35.0%
CATATGTCGTAAACAGAACT+AGG - chr7_4:50300404-50300423 None:intergenic 35.0%
!! GTTTTTCAGTCAGCAACATA+TGG + chr7_4:50300458-50300477 Msa1160410:intron 35.0%
!! ATATGGCAAGGTTCTTGTTT+TGG + chr7_4:50300475-50300494 Msa1160410:CDS 35.0%
ATGAGTGATCATCTCTTGAT+AGG - chr7_4:50300537-50300556 None:intergenic 35.0%
!!! TCAAATACCTTTTTGGGGTT+TGG - chr7_4:50300578-50300597 None:intergenic 35.0%
ATATAAAACAAGTGTGCAGC+AGG - chr7_4:50300817-50300836 None:intergenic 35.0%
!! GGTTACAGTTAATGGATTTG+AGG + chr7_4:50300878-50300897 Msa1160410:intron 35.0%
!!! TTTTAAGAAGATGATGGTGG+TGG - chr7_4:50300964-50300983 None:intergenic 35.0%
!!! GAGTTTTAAGAAGATGATGG+TGG - chr7_4:50300967-50300986 None:intergenic 35.0%
AAACTCATGACAGTGATAGA+TGG + chr7_4:50301013-50301032 Msa1160410:intron 35.0%
ATTAGTAAGGGAGATGTTCT+AGG - chr7_4:50301242-50301261 None:intergenic 35.0%
!! CTTTTTCTATGATGGAGTGT+GGG - chr7_4:50301379-50301398 None:intergenic 35.0%
!! ACTTTTTCTATGATGGAGTG+TGG - chr7_4:50301380-50301399 None:intergenic 35.0%
TGAAAGAAGGACTAAACTAG+TGG + chr7_4:50301468-50301487 Msa1160410:three_prime_UTR 35.0%
CAGGTGATCATATATATGCT+AGG - chr7_4:50301721-50301740 None:intergenic 35.0%
GAGTGCTATCTAAAAAATGC+AGG - chr7_4:50301740-50301759 None:intergenic 35.0%
ATTCTAGACTCTCGTCTAAT+TGG - chr7_4:50301939-50301958 None:intergenic 35.0%
GCTGGAAGTTTAATTGAATC+AGG + chr7_4:50301977-50301996 Msa1160410:three_prime_UTR 35.0%
TGGCTATTGATCACTACTAT+TGG - chr7_4:50302117-50302136 None:intergenic 35.0%
!! GCAGAATTTATGAAAGTACC+AGG + chr7_4:50302179-50302198 Msa1160410:three_prime_UTR 35.0%
CCTCAAAAATGTCCTCAAAA+TGG - chr7_4:50302371-50302390 None:intergenic 35.0%
!! CCATTTTGAGGACATTTTTG+AGG + chr7_4:50302368-50302387 Msa1160410:three_prime_UTR 35.0%
! TTTTCATTGGACCAGCTAAT+AGG - chr7_4:50302507-50302526 None:intergenic 35.0%
TGTAGGATAATGTGGTTTGA+AGG + chr7_4:50302750-50302769 Msa1160410:three_prime_UTR 35.0%
GTAGGATAATGTGGTTTGAA+GGG + chr7_4:50302751-50302770 Msa1160410:three_prime_UTR 35.0%
TTGAAGGGAGTGACTTTAAT+TGG + chr7_4:50302766-50302785 Msa1160410:three_prime_UTR 35.0%
! GTACCACTGTGTTTTCATTA+TGG + chr7_4:50302860-50302879 Msa1160410:intron 35.0%
CATCATTGGATTCATTGTTG+TGG + chr7_4:50302921-50302940 Msa1160410:three_prime_UTR 35.0%
!! GCTTTCGAGTTTGTGTTTAT+TGG + chr7_4:50302970-50302989 Msa1160410:three_prime_UTR 35.0%
GTTTACATGACCATACATTG+TGG + chr7_4:50303303-50303322 Msa1160410:three_prime_UTR 35.0%
TAATTCTGGACCACAATGTA+TGG - chr7_4:50303316-50303335 None:intergenic 35.0%
AATCGCAGTAAGATGTTGTG+CGG - chr7_4:50299951-50299970 None:intergenic 40.0%
TAACTGAAGCTACATGTACC+TGG - chr7_4:50300353-50300372 None:intergenic 40.0%
ACAGAACTAGGACTATGAAG+GGG - chr7_4:50300392-50300411 None:intergenic 40.0%
!!! GCAAGGTTCTTGTTTTGGAT+GGG + chr7_4:50300480-50300499 Msa1160410:CDS 40.0%
!!! CAAGGTTCTTGTTTTGGATG+GGG + chr7_4:50300481-50300500 Msa1160410:CDS 40.0%
CTCAATTCCAAACCCCAAAA+AGG + chr7_4:50300568-50300587 Msa1160410:CDS 40.0%
!! TAATGGATTTGAGGGAGAAG+AGG + chr7_4:50300887-50300906 Msa1160410:intron 40.0%
! AATGGATTTGAGGGAGAAGA+GGG + chr7_4:50300888-50300907 Msa1160410:intron 40.0%
AACTACTCACCAGTGTTCTA+AGG + chr7_4:50300926-50300945 Msa1160410:intron 40.0%
TTGTGGATTCCTTAGAACAC+TGG - chr7_4:50300938-50300957 None:intergenic 40.0%
GCTAGTGAGTGTAAACCTTA+TGG - chr7_4:50301762-50301781 None:intergenic 40.0%
!! ACTATTTGCTGTGGGTTATC+TGG - chr7_4:50301861-50301880 None:intergenic 40.0%
TCTGTTTGTGTTCTGTTCCA+TGG + chr7_4:50302606-50302625 Msa1160410:three_prime_UTR 40.0%
TGCAAGGAATCTCTTAACCA+TGG - chr7_4:50302626-50302645 None:intergenic 40.0%
! GAAGGCTGTCTTTGTTATGT+AGG + chr7_4:50302733-50302752 Msa1160410:three_prime_UTR 40.0%
CTCAATCTTAGGACTGTTTC+AGG + chr7_4:50302887-50302906 Msa1160410:intron 40.0%
!! CTTAGGACTGTTTCAGGTTA+TGG + chr7_4:50302893-50302912 Msa1160410:intron 40.0%
!! TCTATTTTTCCTCCTTGCCA+AGG - chr7_4:50303156-50303175 None:intergenic 40.0%
TGGCAAGGAGGAAAAATAGA+AGG + chr7_4:50303156-50303175 Msa1160410:three_prime_UTR 40.0%
AGCTGAAATGTGTGTGGGTT+TGG - chr7_4:50299871-50299890 None:intergenic 45.0%
ATGAGGATCTAGGAGTTGGA+AGG - chr7_4:50299977-50299996 None:intergenic 45.0%
ATCAAGCAGGATGAGGATCT+AGG - chr7_4:50299987-50300006 None:intergenic 45.0%
CTCATCCTGCTTGATGCTTT+GGG + chr7_4:50299992-50300011 Msa1160410:five_prime_UTR 45.0%
TCAGGATGTTATGGTCTTCC+AGG + chr7_4:50300332-50300351 Msa1160410:CDS 45.0%
TCAGTCAGCAACATATGGCA+AGG + chr7_4:50300463-50300482 Msa1160410:intron 45.0%
!!! GGCAAGGTTCTTGTTTTGGA+TGG + chr7_4:50300479-50300498 Msa1160410:CDS 45.0%
GGTCATTCAGCTCACAGAAT+GGG + chr7_4:50300502-50300521 Msa1160410:CDS 45.0%
! GGGTTTGGAATTGAGCAAAG+AGG - chr7_4:50300563-50300582 None:intergenic 45.0%
AGAAGAGGGATGAAGAGTTG+AGG + chr7_4:50300902-50300921 Msa1160410:intron 45.0%
TGAGGTATGATCGCAAGTAC+TGG + chr7_4:50301524-50301543 Msa1160410:three_prime_UTR 45.0%
GCAAAGCAGAACAACTTGGA+AGG - chr7_4:50302206-50302225 None:intergenic 45.0%
GTCAGCAAAGCAGAACAACT+TGG - chr7_4:50302210-50302229 None:intergenic 45.0%
TCATTGGACCAGCTAATAGG+AGG - chr7_4:50302504-50302523 None:intergenic 45.0%
TCCTTGCATTAAGGCCTTAG+TGG + chr7_4:50302638-50302657 Msa1160410:three_prime_UTR 45.0%
! GGATTAACACAGTGCCACTA+AGG - chr7_4:50302655-50302674 None:intergenic 45.0%
!! AGGTTATGGTGCTGCATCAT+TGG + chr7_4:50302907-50302926 Msa1160410:intron 45.0%
AGCCATGTTGCCAGATGAAA+TGG + chr7_4:50303129-50303148 Msa1160410:intron 45.0%
GCCATGTTGCCAGATGAAAT+GGG + chr7_4:50303130-50303149 Msa1160410:intron 45.0%
TCGCGAGCTGAAATGTGTGT+GGG - chr7_4:50299876-50299895 None:intergenic 50.0%
AGGATCTAGGAGTTGGAAGG+TGG - chr7_4:50299974-50299993 None:intergenic 50.0%
CAGGATGAGGATCTAGGAGT+TGG - chr7_4:50299981-50300000 None:intergenic 50.0%
! CCAAAGCATCAAGCAGGATG+AGG - chr7_4:50299994-50300013 None:intergenic 50.0%
CCTCATCCTGCTTGATGCTT+TGG + chr7_4:50299991-50300010 Msa1160410:five_prime_UTR 50.0%
! CAACACCCAAAGCATCAAGC+AGG - chr7_4:50300000-50300019 None:intergenic 50.0%
GGGTCATTCAGCTCACAGAA+TGG + chr7_4:50300501-50300520 Msa1160410:CDS 50.0%
!! GATGATGGTGGTGGTTGTTG+TGG - chr7_4:50300955-50300974 None:intergenic 50.0%
GGACTAAACTAGTGGAGACC+AGG + chr7_4:50301476-50301495 Msa1160410:three_prime_UTR 50.0%
CAGAACAACTTGGAAGGTCC+TGG - chr7_4:50302200-50302219 None:intergenic 50.0%
!! GCTAATAGGAGGCATCTCCA+GGG - chr7_4:50302493-50302512 None:intergenic 50.0%
!! AGCTAATAGGAGGCATCTCC+AGG - chr7_4:50302494-50302513 None:intergenic 50.0%
TGGAGATGCCTCCTATTAGC+TGG + chr7_4:50302493-50302512 Msa1160410:three_prime_UTR 50.0%
GCCACTAAGGCCTTAATGCA+AGG - chr7_4:50302642-50302661 None:intergenic 50.0%
GCCCATTTCATCTGGCAACA+TGG - chr7_4:50303134-50303153 None:intergenic 50.0%
TTGCCAGATGAAATGGGCCT+TGG + chr7_4:50303136-50303155 Msa1160410:three_prime_UTR 50.0%
TTGCCAAGGCCCATTTCATC+TGG - chr7_4:50303142-50303161 None:intergenic 50.0%
AGATGAAATGGGCCTTGGCA+AGG + chr7_4:50303141-50303160 Msa1160410:three_prime_UTR 50.0%
GTCGCGAGCTGAAATGTGTG+TGG - chr7_4:50299877-50299896 None:intergenic 55.0%
CAGTAAGATGTTGTGCGGCG+CGG - chr7_4:50299946-50299965 None:intergenic 55.0%
AGCAGGAGTTAGCACGGTGA+CGG - chr7_4:50300800-50300819 None:intergenic 55.0%
GTGTGCAGCAGGAGTTAGCA+CGG - chr7_4:50300806-50300825 None:intergenic 55.0%
TGAAATGGGCCTTGGCAAGG+AGG + chr7_4:50303144-50303163 Msa1160410:three_prime_UTR 55.0%
AGATGTTGTGCGGCGCGGCT+TGG - chr7_4:50299941-50299960 None:intergenic 65.0%
!! GCGCGGCTTGGTGGTGATGG+AGG - chr7_4:50299929-50299948 None:intergenic 70.0%
! TGTTGTGCGGCGCGGCTTGG+TGG - chr7_4:50299938-50299957 None:intergenic 70.0%
!! GCGGCGCGGCTTGGTGGTGA+TGG - chr7_4:50299932-50299951 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 50299808 50303401 50299808 ID=Msa1160410;Name=Msa1160410
chr7_4 mRNA 50299808 50303401 50299808 ID=Msa1160410-mRNA-1;Parent=Msa1160410;Name=Msa1160410-mRNA-1;_AED=0.07;_eAED=0.07;_QI=252|1|1|1|0.6|0.66|6|1852|85
chr7_4 exon 50299808 50300124 50299808 ID=Msa1160410-mRNA-1:exon:13961;Parent=Msa1160410-mRNA-1
chr7_4 exon 50300290 50300353 50300290 ID=Msa1160410-mRNA-1:exon:13962;Parent=Msa1160410-mRNA-1
chr7_4 exon 50300467 50300589 50300467 ID=Msa1160410-mRNA-1:exon:13963;Parent=Msa1160410-mRNA-1
chr7_4 exon 50301369 50302811 50301369 ID=Msa1160410-mRNA-1:exon:13964;Parent=Msa1160410-mRNA-1
chr7_4 exon 50302909 50303052 50302909 ID=Msa1160410-mRNA-1:exon:13965;Parent=Msa1160410-mRNA-1
chr7_4 exon 50303131 50303401 50303131 ID=Msa1160410-mRNA-1:exon:13966;Parent=Msa1160410-mRNA-1
chr7_4 five_prime_UTR 50299808 50300059 50299808 ID=Msa1160410-mRNA-1:five_prime_utr;Parent=Msa1160410-mRNA-1
chr7_4 CDS 50300060 50300124 50300060 ID=Msa1160410-mRNA-1:cds;Parent=Msa1160410-mRNA-1
chr7_4 CDS 50300290 50300353 50300290 ID=Msa1160410-mRNA-1:cds;Parent=Msa1160410-mRNA-1
chr7_4 CDS 50300467 50300589 50300467 ID=Msa1160410-mRNA-1:cds;Parent=Msa1160410-mRNA-1
chr7_4 CDS 50301369 50301374 50301369 ID=Msa1160410-mRNA-1:cds;Parent=Msa1160410-mRNA-1
chr7_4 three_prime_UTR 50301375 50302811 50301375 ID=Msa1160410-mRNA-1:three_prime_utr;Parent=Msa1160410-mRNA-1
chr7_4 three_prime_UTR 50302909 50303052 50302909 ID=Msa1160410-mRNA-1:three_prime_utr;Parent=Msa1160410-mRNA-1
chr7_4 three_prime_UTR 50303131 50303401 50303131 ID=Msa1160410-mRNA-1:three_prime_utr;Parent=Msa1160410-mRNA-1
Gene Sequence

>Msa1160410

ATGACGAATCTTCATCTCGAAGATCTACATGAAGCCGATCTATATGAATCTGTCACCATTAACAAGTATAATTGGTTCAATTTATTGTTTCAAGGAAAATCTGATTATCAGGATGTTATGGTCTTCCAGTCAGCAACATATGGCAAGGTTCTTGTTTTGGATGGGGTCATTCAGCTCACAGAATGGGACGAGTGTGCCTATCAAGAGATGATCACTCATCTTCCTCTTTGCTCAATTCCAAACCCCAAAAAGGTTTGA
Protein sequence

>Msa1160410

MTNLHLEDLHEADLYESVTINKYNWFNLLFQGKSDYQDVMVFQSATYGKVLVLDGVIQLTEWDECAYQEMITHLPLCSIPNPKKV*