AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1161660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1161660 MtrunA17_Chr7g0254951 93.182 220 12 1 19 235 1 220 1.34e-138 389
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1161660 AT3G56820.1 55.140 214 74 5 48 244 12 220 6.64e-72 219
Msa1161660 AT3G56820.3 51.402 214 66 6 48 244 12 204 1.60e-60 189
Msa1161660 AT3G56820.2 50.340 147 53 3 48 177 12 155 2.89e-38 131

Find 95 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAAGTTGTTCTCTTTAT+AGG 0.149046 7_4:+51831734 Msa1161660:exon
GCACTTTCTTCCAGCATTTC+AGG 0.170527 7_4:+51830047 Msa1161660:CDS
TTTATGCCTTAGTCTATTAA+AGG 0.217854 7_4:+51831951 Msa1161660:three_prime_UTR
TAGCTCTAACGTCGTCGTTT+GGG 0.253390 7_4:-51829944 None:intergenic
GGGTGCTGCTATCAAGTTTA+GGG 0.274684 7_4:+51830172 Msa1161660:CDS
GTTCGGAGTGCTTACTTTAT+AGG 0.282848 7_4:+51831650 Msa1161660:CDS
GGGGTGCTGCTATCAAGTTT+AGG 0.298006 7_4:+51830171 Msa1161660:CDS
AGGATGGTGGTATGGTGATT+TGG 0.331597 7_4:-51829758 None:intergenic
TTAGCTCTAACGTCGTCGTT+TGG 0.349756 7_4:-51829945 None:intergenic
CAAGTACGGACTTTAATGTT+TGG 0.354005 7_4:+51831705 Msa1161660:CDS
CCATCTTCCTCTTCCTCGCT+TGG 0.355448 7_4:+51829874 Msa1161660:CDS
ACTTGTGCTTCTCTTCATCT+TGG 0.370893 7_4:+51830080 Msa1161660:CDS
GTGGTAGTGGTGGTTCTCTC+TGG 0.371072 7_4:-51829678 None:intergenic
TTCCTTCCTTTAATAGACTA+AGG 0.372920 7_4:-51831957 None:intergenic
AACAGCGAGGTAACTGATAT+TGG 0.393984 7_4:+51831539 Msa1161660:CDS
TATAGTTTATGATACTATCT+TGG 0.398381 7_4:-51831676 None:intergenic
TTTACAATCAGTACAAGTTC+AGG 0.405213 7_4:-51832001 None:intergenic
GGAGAGAGTGAGGTCTTTGA+AGG 0.412613 7_4:-51829839 None:intergenic
GAAGAAAGTGCGAGAGCAAT+AGG 0.413504 7_4:-51830035 None:intergenic
CCGTTGAAACATGGCGGAAG+TGG 0.425519 7_4:-51829790 None:intergenic
TTATTATATAGACATCTAAA+AGG 0.426582 7_4:-51831850 None:intergenic
AACAGCTCCACCTGAAATGC+TGG 0.433121 7_4:-51830057 None:intergenic
GGAAGATGGAGAGGACGAAA+GGG 0.435556 7_4:-51829860 None:intergenic
AGGAAGATGGAGAGGACGAA+AGG 0.436973 7_4:-51829861 None:intergenic
GGTGGTATGGTGATTTGGAT+TGG 0.443968 7_4:-51829753 None:intergenic
GTAAATAGGCAACGCAGATT+AGG 0.445935 7_4:-51832077 None:intergenic
GGCGGAAGTGGAGGAGGAGG+AGG 0.445946 7_4:-51829778 None:intergenic
CATCTTGGTGAAATTCGACC+TGG 0.453204 7_4:+51830095 Msa1161660:CDS
GGAATGAGGTGACGAGGGAG+GGG 0.455393 7_4:-51829818 None:intergenic
TGGTCTTCGCGGTGTTGCGC+CGG 0.464153 7_4:-51829733 None:intergenic
GAGAGATCCAAGCGAGGAAG+AGG 0.466239 7_4:-51829881 None:intergenic
TGACATTGCGTTGCTGCTAC+TGG 0.469672 7_4:-51829703 None:intergenic
CCACGATTATCCTTGGCAAC+AGG 0.473427 7_4:-51829999 None:intergenic
TTTGGGGAAGAGAGAGGGAG+TGG 0.476012 7_4:-51829927 None:intergenic
CATGGCGGAAGTGGAGGAGG+AGG 0.480351 7_4:-51829781 None:intergenic
AAAAGAAAGACGGAATGAAA+GGG 0.482053 7_4:+51831921 Msa1161660:three_prime_UTR
GGAGGAGGAGGATGGTGGTA+TGG 0.487732 7_4:-51829766 None:intergenic
AGGAATGAGGTGACGAGGGA+GGG 0.496689 7_4:-51829819 None:intergenic
GCTGCTACTGGTGGTGGTAG+TGG 0.498014 7_4:-51829691 None:intergenic
AAAGCATGTGCAGTATGAGC+AGG 0.498771 7_4:-51831611 None:intergenic
GGCTATGCTGAGGTGATCAT+TGG 0.499112 7_4:+51831560 Msa1161660:CDS
CCAAGCGAGGAAGAGGAAGA+TGG 0.501259 7_4:-51829874 None:intergenic
TAGTGAACATAGATCCGGTT+CGG 0.501356 7_4:+51831633 Msa1161660:CDS
AACGCAATGTCATCAACAAC+CGG 0.502607 7_4:+51829714 Msa1161660:CDS
CTTTCCTTCTCCTGTTGCCA+AGG 0.502984 7_4:+51829989 Msa1161660:CDS
TTAGTCTATTAAAGGAAGGA+AGG 0.509234 7_4:+51831959 Msa1161660:three_prime_UTR
ATAAAGTAAGCACTCCGAAC+CGG 0.512043 7_4:-51831647 None:intergenic
TGCGTTGCTGCTACTGGTGG+TGG 0.513992 7_4:-51829697 None:intergenic
TGCTTTAGTGAACATAGATC+CGG 0.514575 7_4:+51831628 Msa1161660:CDS
TGATTTCCTCTCAACTTCCC+AGG 0.523403 7_4:-51830113 None:intergenic
GTGAGGTCTTTGAAGGAATG+AGG 0.525795 7_4:-51829832 None:intergenic
AGCTCTAACGTCGTCGTTTG+GGG 0.528964 7_4:-51829943 None:intergenic
AAACATGGCGGAAGTGGAGG+AGG 0.531130 7_4:-51829784 None:intergenic
CCACTTCCGCCATGTTTCAA+CGG 0.538029 7_4:+51829790 Msa1161660:CDS
AATCCAACCACGATTATCCT+TGG 0.538228 7_4:-51830006 None:intergenic
AACATTAAAGTCCGTACTTG+AGG 0.548423 7_4:-51831702 None:intergenic
ATGAGGTGACGAGGGAGGGG+CGG 0.548655 7_4:-51829815 None:intergenic
AAACTATAATGCCTCAAGTA+CGG 0.551251 7_4:+51831691 Msa1161660:CDS
CTTTCTTCCAGCATTTCAGG+TGG 0.551639 7_4:+51830050 Msa1161660:CDS
TTATCCTTGGCAACAGGAGA+AGG 0.552794 7_4:-51829993 None:intergenic
AACTTGTACTGATTGTAAAG+AGG 0.554257 7_4:+51832005 Msa1161660:three_prime_UTR
TTCATCAAGAGGAATGTGAT+GGG 0.554812 7_4:-51831761 None:intergenic
TGCCTTAGTCTATTAAAGGA+AGG 0.558946 7_4:+51831955 Msa1161660:three_prime_UTR
GCTACTGGTGGTGGTAGTGG+TGG 0.566505 7_4:-51829688 None:intergenic
TTGAAACATGGCGGAAGTGG+AGG 0.573268 7_4:-51829787 None:intergenic
GGCAACAGGAGAAGGAAAGC+GGG 0.576759 7_4:-51829985 None:intergenic
GGGGCGGATCCGTTGAAACA+TGG 0.576935 7_4:-51829799 None:intergenic
TTTCATCAAGAGGAATGTGA+TGG 0.579388 7_4:-51831762 None:intergenic
AAGGAATGAGGTGACGAGGG+AGG 0.581491 7_4:-51829820 None:intergenic
ATTCGACCTGGGAAGTTGAG+AGG 0.582502 7_4:+51830107 Msa1161660:CDS
CGAGGAAGAGGAAGATGGAG+AGG 0.582911 7_4:-51829869 None:intergenic
AGGACGAAAGGGAGAGAGTG+AGG 0.583353 7_4:-51829849 None:intergenic
CGTCGTTTGGGGAAGAGAGA+GGG 0.585037 7_4:-51829932 None:intergenic
TAGTCTATTAAAGGAAGGAA+GGG 0.588168 7_4:+51831960 Msa1161660:three_prime_UTR
AAGAGTAAAGAATACACTAT+CGG 0.589376 7_4:+51829634 Msa1161660:five_prime_UTR
TTGCCAAGGATAATCGTGGT+TGG 0.591704 7_4:+51830003 Msa1161660:CDS
ACATCTTAGATTTCATCAAG+AGG 0.594395 7_4:-51831772 None:intergenic
GTGATTTGGATTGGTCTTCG+CGG 0.598960 7_4:-51829744 None:intergenic
ATCTTGGTGAAATTCGACCT+GGG 0.602964 7_4:+51830096 Msa1161660:CDS
TCGTCGTTTGGGGAAGAGAG+AGG 0.608845 7_4:-51829933 None:intergenic
AAAGAAAGACGGAATGAAAG+GGG 0.614950 7_4:+51831922 Msa1161660:three_prime_UTR
CCTGTTGCCAAGGATAATCG+TGG 0.632768 7_4:+51829999 Msa1161660:CDS
GATGAGAAGAGCGATATGAA+CGG 0.633747 7_4:-51829905 None:intergenic
AAGCATGTGCAGTATGAGCA+GGG 0.642060 7_4:-51831610 None:intergenic
TGGCAACAGGAGAAGGAAAG+CGG 0.642937 7_4:-51829986 None:intergenic
TTTGAAGGAATGAGGTGACG+AGG 0.649908 7_4:-51829824 None:intergenic
TTGAAGGAATGAGGTGACGA+GGG 0.664590 7_4:-51829823 None:intergenic
TCATCAAGAGGAATGTGATG+GGG 0.666815 7_4:-51831760 None:intergenic
CATTGCGTTGCTGCTACTGG+TGG 0.668867 7_4:-51829700 None:intergenic
AACTGATATTGGCTATGCTG+AGG 0.679963 7_4:+51831550 Msa1161660:CDS
AAGAAAGACGGAATGAAAGG+GGG 0.685351 7_4:+51831923 Msa1161660:three_prime_UTR
GGTGATCATTGGCAGAGATG+AGG 0.708921 7_4:+51831571 Msa1161660:CDS
AACGGAGAGAGATCCAAGCG+AGG 0.715907 7_4:-51829887 None:intergenic
GCGGATCCGTTGAAACATGG+CGG 0.736324 7_4:-51829796 None:intergenic
TTTGCAGGTGGAAAACAGCG+AGG 0.739005 7_4:+51831526 Msa1161660:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATTATTATGATTTTGT+TGG + chr7_4:51831805-51831824 Msa1161660:three_prime_UTR 10.0%
!! AACTATTAATTATACATCAA+TGG - chr7_4:51830356-51830375 None:intergenic 15.0%
!!! AAATATCAACTTAAGTTTTT+TGG + chr7_4:51831277-51831296 Msa1161660:intron 15.0%
!! TTATTATATAGACATCTAAA+AGG - chr7_4:51831853-51831872 None:intergenic 15.0%
!!! TCATTATCTGTTTTTTGTTT+TGG - chr7_4:51829614-51829633 None:intergenic 20.0%
!! AGATAAAATGCTAGTTATAT+TGG + chr7_4:51830259-51830278 Msa1161660:intron 20.0%
!!! GTTATTACTGAAAAAGTTTT+TGG + chr7_4:51830622-51830641 Msa1161660:intron 20.0%
!!! TATTTTGCAAACCAATTTTA+TGG + chr7_4:51830875-51830894 Msa1161660:intron 20.0%
!! AAAAGATTCTTCATTTCATT+GGG - chr7_4:51831454-51831473 None:intergenic 20.0%
!! AAAAAGATTCTTCATTTCAT+TGG - chr7_4:51831455-51831474 None:intergenic 20.0%
!! TATAGTTTATGATACTATCT+TGG - chr7_4:51831679-51831698 None:intergenic 20.0%
! AAGAGTAAAGAATACACTAT+CGG + chr7_4:51829634-51829653 Msa1161660:five_prime_UTR 25.0%
!! TAATGAGACTTTTGTTCTTT+GGG + chr7_4:51830151-51830170 Msa1161660:CDS 25.0%
! ATTAATTTAAGAGAGAGGAA+AGG - chr7_4:51830206-51830225 None:intergenic 25.0%
! TCCTCTCTCTTAAATTAATT+TGG + chr7_4:51830207-51830226 Msa1161660:intron 25.0%
! ACCAAATTAATTTAAGAGAG+AGG - chr7_4:51830211-51830230 None:intergenic 25.0%
! TTTCAAAACCATGTTTGAAT+TGG - chr7_4:51830240-51830259 None:intergenic 25.0%
! TGAATGTTCGAATTAACATT+TGG + chr7_4:51830289-51830308 Msa1161660:intron 25.0%
! TAGTCCTTAACTATTATGTT+AGG + chr7_4:51830514-51830533 Msa1161660:intron 25.0%
!!! GATTTTAAATTGTTGACTTG+TGG + chr7_4:51830542-51830561 Msa1161660:intron 25.0%
! AATTGTGTTTGATGTTATAC+CGG + chr7_4:51830589-51830608 Msa1161660:intron 25.0%
! AGTAATAACTATAGTATACC+CGG - chr7_4:51830611-51830630 None:intergenic 25.0%
! TAGCATCACTTTATATCATT+GGG - chr7_4:51830686-51830705 None:intergenic 25.0%
! TTAGCATCACTTTATATCAT+TGG - chr7_4:51830687-51830706 None:intergenic 25.0%
! AAGCAAACACTAAAAATGTT+TGG + chr7_4:51830848-51830867 Msa1161660:intron 25.0%
! CAAATATAAGTTTGCAAACT+AGG - chr7_4:51831073-51831092 None:intergenic 25.0%
! TTGTTATGCAAAATGAAGTT+GGG - chr7_4:51831097-51831116 None:intergenic 25.0%
! ATATTTAGTATGTACATGTG+TGG - chr7_4:51831263-51831282 None:intergenic 25.0%
!! TTTCATTGGGATTGTAATTT+TGG - chr7_4:51831441-51831460 None:intergenic 25.0%
!!! TGAAGAATCTTTTTTGTTGT+TGG + chr7_4:51831461-51831480 Msa1161660:intron 25.0%
!!! CTTTAAGTTGTTCTCTTTAT+AGG + chr7_4:51831734-51831753 Msa1161660:exon 25.0%
! TTTATGCCTTAGTCTATTAA+AGG + chr7_4:51831951-51831970 Msa1161660:three_prime_UTR 25.0%
!! ACATTTTCAGAGTTTAACAA+GGG - chr7_4:51832053-51832072 None:intergenic 25.0%
! GTAATGAGACTTTTGTTCTT+TGG + chr7_4:51830150-51830169 Msa1161660:CDS 30.0%
! AATGAGACTTTTGTTCTTTG+GGG + chr7_4:51830152-51830171 Msa1161660:CDS 30.0%
TTGGTTCCTCTAATTCAATT+TGG + chr7_4:51830308-51830327 Msa1161660:intron 30.0%
TACACTCCAAATTGAATTAG+AGG - chr7_4:51830317-51830336 None:intergenic 30.0%
ACAGCCTAACATAATAGTTA+AGG - chr7_4:51830521-51830540 None:intergenic 30.0%
ATTGTGTTTGATGTTATACC+GGG + chr7_4:51830590-51830609 Msa1161660:intron 30.0%
CATTAATAGATCCTAGTTAC+AGG - chr7_4:51830756-51830775 None:intergenic 30.0%
AAGATGAAAATTACGCTCTA+TGG + chr7_4:51830799-51830818 Msa1161660:intron 30.0%
AATACTTATTGATCCAATGG+TGG - chr7_4:51830948-51830967 None:intergenic 30.0%
ACATATCACATTTGATCCAA+TGG - chr7_4:51830972-51830991 None:intergenic 30.0%
!! CAAGTTTGTTTTCTACCATT+AGG - chr7_4:51831008-51831027 None:intergenic 30.0%
GTTGTTATGCAAAATGAAGT+TGG - chr7_4:51831098-51831117 None:intergenic 30.0%
TTAAAATGGAAACAAAGGGA+AGG - chr7_4:51831120-51831139 None:intergenic 30.0%
! CGGTTTAAAATGGAAACAAA+GGG - chr7_4:51831124-51831143 None:intergenic 30.0%
! TCGGTTTAAAATGGAAACAA+AGG - chr7_4:51831125-51831144 None:intergenic 30.0%
!!! TTGTTCAGTTGTTTGGTTTT+CGG - chr7_4:51831186-51831205 None:intergenic 30.0%
TGCTGTGTTAAAATAAGCTA+CGG + chr7_4:51831216-51831235 Msa1161660:intron 30.0%
!! CTCAAAATGATTAGCTTATG+CGG + chr7_4:51831376-51831395 Msa1161660:intron 30.0%
AAACTATAATGCCTCAAGTA+CGG + chr7_4:51831691-51831710 Msa1161660:CDS 30.0%
ACATCTTAGATTTCATCAAG+AGG - chr7_4:51831775-51831794 None:intergenic 30.0%
! ACTCAGTATGAGCATTTTAT+AGG + chr7_4:51831893-51831912 Msa1161660:three_prime_UTR 30.0%
! CTCAGTATGAGCATTTTATA+GGG + chr7_4:51831894-51831913 Msa1161660:three_prime_UTR 30.0%
ATAGGGTACAAAAAGAAAGA+CGG + chr7_4:51831911-51831930 Msa1161660:three_prime_UTR 30.0%
AAAAAGAAAGACGGAATGAA+AGG + chr7_4:51831920-51831939 Msa1161660:three_prime_UTR 30.0%
AAAAGAAAGACGGAATGAAA+GGG + chr7_4:51831921-51831940 Msa1161660:three_prime_UTR 30.0%
TTCCTTCCTTTAATAGACTA+AGG - chr7_4:51831960-51831979 None:intergenic 30.0%
TTAGTCTATTAAAGGAAGGA+AGG + chr7_4:51831959-51831978 Msa1161660:three_prime_UTR 30.0%
TAGTCTATTAAAGGAAGGAA+GGG + chr7_4:51831960-51831979 Msa1161660:three_prime_UTR 30.0%
TTTACAATCAGTACAAGTTC+AGG - chr7_4:51832004-51832023 None:intergenic 30.0%
!! AACTTGTACTGATTGTAAAG+AGG + chr7_4:51832005-51832024 Msa1161660:three_prime_UTR 30.0%
! CACATTTTCAGAGTTTAACA+AGG - chr7_4:51832054-51832073 None:intergenic 30.0%
GTTCATTGCCAATTCAAACA+TGG + chr7_4:51830229-51830248 Msa1161660:intron 35.0%
! CATCAATGGTTGATATCATG+CGG - chr7_4:51830342-51830361 None:intergenic 35.0%
GATCAACCATGTTGACATAT+CGG + chr7_4:51830406-51830425 Msa1161660:intron 35.0%
ATTGAAAGAACCCTTCGTTA+AGG - chr7_4:51830476-51830495 None:intergenic 35.0%
TTTAGTGTTTGCTTGATCGT+AGG - chr7_4:51830842-51830861 None:intergenic 35.0%
TCACTTGTGCACCATAAAAT+TGG - chr7_4:51830889-51830908 None:intergenic 35.0%
! AACTTGTTTGTTTCCACCAT+TGG + chr7_4:51830932-51830951 Msa1161660:intron 35.0%
GGCAATACTTATTGATCCAA+TGG - chr7_4:51830951-51830970 None:intergenic 35.0%
! GGATCAATAAGTATTGCCAT+TGG + chr7_4:51830953-51830972 Msa1161660:intron 35.0%
GTAGAAAACAAACTTGTGCA+TGG + chr7_4:51831012-51831031 Msa1161660:intron 35.0%
CCTTCAAACTCGGTTTAAAA+TGG - chr7_4:51831134-51831153 None:intergenic 35.0%
! CCATTTTAAACCGAGTTTGA+AGG + chr7_4:51831131-51831150 Msa1161660:intron 35.0%
CTAACTCTTGTTCAGTTGTT+TGG - chr7_4:51831193-51831212 None:intergenic 35.0%
GCTGTGTTAAAATAAGCTAC+GGG + chr7_4:51831217-51831236 Msa1161660:intron 35.0%
CTGTGTTAAAATAAGCTACG+GGG + chr7_4:51831218-51831237 Msa1161660:intron 35.0%
TAGTATGTACATGTGTGGTT+GGG - chr7_4:51831258-51831277 None:intergenic 35.0%
TTAGTATGTACATGTGTGGT+TGG - chr7_4:51831259-51831278 None:intergenic 35.0%
TGCTTTAGTGAACATAGATC+CGG + chr7_4:51831628-51831647 Msa1161660:CDS 35.0%
AACATTAAAGTCCGTACTTG+AGG - chr7_4:51831705-51831724 None:intergenic 35.0%
CAAGTACGGACTTTAATGTT+TGG + chr7_4:51831705-51831724 Msa1161660:CDS 35.0%
TTCATCAAGAGGAATGTGAT+GGG - chr7_4:51831764-51831783 None:intergenic 35.0%
TTTCATCAAGAGGAATGTGA+TGG - chr7_4:51831765-51831784 None:intergenic 35.0%
AAAGAAAGACGGAATGAAAG+GGG + chr7_4:51831922-51831941 Msa1161660:three_prime_UTR 35.0%
TGCCTTAGTCTATTAAAGGA+AGG + chr7_4:51831955-51831974 Msa1161660:three_prime_UTR 35.0%
AACGCAATGTCATCAACAAC+CGG + chr7_4:51829714-51829733 Msa1161660:CDS 40.0%
GATGAGAAGAGCGATATGAA+CGG - chr7_4:51829908-51829927 None:intergenic 40.0%
AATCCAACCACGATTATCCT+TGG - chr7_4:51830009-51830028 None:intergenic 40.0%
ACTTGTGCTTCTCTTCATCT+TGG + chr7_4:51830080-51830099 Msa1161660:CDS 40.0%
ATCTTGGTGAAATTCGACCT+GGG + chr7_4:51830096-51830115 Msa1161660:CDS 40.0%
ATGTCAACATGGTTGATCGT+CGG - chr7_4:51830404-51830423 None:intergenic 40.0%
CAATGACCGATATGTCAACA+TGG - chr7_4:51830415-51830434 None:intergenic 40.0%
ATGAGCCAAATCCTTAACGA+AGG + chr7_4:51830462-51830481 Msa1161660:intron 40.0%
TGAGCCAAATCCTTAACGAA+GGG + chr7_4:51830463-51830482 Msa1161660:intron 40.0%
AGAACCCTTCGTTAAGGATT+TGG - chr7_4:51830470-51830489 None:intergenic 40.0%
!!! GAAAAAGTTTTTGGTCCTGC+TGG + chr7_4:51830631-51830650 Msa1161660:intron 40.0%
TAAGGAGTTAAACATCCAGC+AGG - chr7_4:51830649-51830668 None:intergenic 40.0%
CTCATGACATCGTTCTTAGA+AGG + chr7_4:51830707-51830726 Msa1161660:intron 40.0%
AGTTACAGGTAGCAGTTTAG+TGG - chr7_4:51830742-51830761 None:intergenic 40.0%
CTAAACTGCTACCTGTAACT+AGG + chr7_4:51830742-51830761 Msa1161660:intron 40.0%
! GAGCGTAATTTTCATCTTGC+TGG - chr7_4:51830797-51830816 None:intergenic 40.0%
GAAACAAACAAGTTCGTGCA+TGG - chr7_4:51830926-51830945 None:intergenic 40.0%
GTCGTGACTTATTGACCTAA+TGG + chr7_4:51830990-51831009 Msa1161660:intron 40.0%
CACATCATTGCCTTCAAACT+CGG - chr7_4:51831144-51831163 None:intergenic 40.0%
!!! TCAGTTGTTTGGTTTTCGGT+TGG - chr7_4:51831182-51831201 None:intergenic 40.0%
GTTCACAGTAATCTGTTTGC+AGG + chr7_4:51831511-51831530 Msa1161660:intron 40.0%
AACAGCGAGGTAACTGATAT+TGG + chr7_4:51831539-51831558 Msa1161660:CDS 40.0%
! AACTGATATTGGCTATGCTG+AGG + chr7_4:51831550-51831569 Msa1161660:CDS 40.0%
TAGTGAACATAGATCCGGTT+CGG + chr7_4:51831633-51831652 Msa1161660:CDS 40.0%
ATAAAGTAAGCACTCCGAAC+CGG - chr7_4:51831650-51831669 None:intergenic 40.0%
! GTTCGGAGTGCTTACTTTAT+AGG + chr7_4:51831650-51831669 Msa1161660:CDS 40.0%
TCATCAAGAGGAATGTGATG+GGG - chr7_4:51831763-51831782 None:intergenic 40.0%
AAGAAAGACGGAATGAAAGG+GGG + chr7_4:51831923-51831942 Msa1161660:three_prime_UTR 40.0%
!! GTGATTTGGATTGGTCTTCG+CGG - chr7_4:51829747-51829766 None:intergenic 45.0%
! GGTGGTATGGTGATTTGGAT+TGG - chr7_4:51829756-51829775 None:intergenic 45.0%
! AGGATGGTGGTATGGTGATT+TGG - chr7_4:51829761-51829780 None:intergenic 45.0%
TTGAAGGAATGAGGTGACGA+GGG - chr7_4:51829826-51829845 None:intergenic 45.0%
TTTGAAGGAATGAGGTGACG+AGG - chr7_4:51829827-51829846 None:intergenic 45.0%
! GTGAGGTCTTTGAAGGAATG+AGG - chr7_4:51829835-51829854 None:intergenic 45.0%
!! TAGCTCTAACGTCGTCGTTT+GGG - chr7_4:51829947-51829966 None:intergenic 45.0%
!! TTAGCTCTAACGTCGTCGTT+TGG - chr7_4:51829948-51829967 None:intergenic 45.0%
TTATCCTTGGCAACAGGAGA+AGG - chr7_4:51829996-51830015 None:intergenic 45.0%
TTGCCAAGGATAATCGTGGT+TGG + chr7_4:51830003-51830022 Msa1161660:CDS 45.0%
! GAAGAAAGTGCGAGAGCAAT+AGG - chr7_4:51830038-51830057 None:intergenic 45.0%
GCACTTTCTTCCAGCATTTC+AGG + chr7_4:51830047-51830066 Msa1161660:CDS 45.0%
CTTTCTTCCAGCATTTCAGG+TGG + chr7_4:51830050-51830069 Msa1161660:CDS 45.0%
CATCTTGGTGAAATTCGACC+TGG + chr7_4:51830095-51830114 Msa1161660:CDS 45.0%
TGATTTCCTCTCAACTTCCC+AGG - chr7_4:51830116-51830135 None:intergenic 45.0%
!! GGGTGCTGCTATCAAGTTTA+GGG + chr7_4:51830172-51830191 Msa1161660:CDS 45.0%
CGAGTTTGAAGGCAATGATG+TGG + chr7_4:51831142-51831161 Msa1161660:intron 45.0%
ATAAGCTACGGGGTTCTCAA+CGG + chr7_4:51831228-51831247 Msa1161660:intron 45.0%
!! GATTAGCTTATGCGGTCTGT+AGG + chr7_4:51831384-51831403 Msa1161660:intron 45.0%
TGCGGTCTGTAGGCAAAATT+TGG + chr7_4:51831394-51831413 Msa1161660:intron 45.0%
GCGGTCTGTAGGCAAAATTT+GGG + chr7_4:51831395-51831414 Msa1161660:intron 45.0%
CACAGTAATCTGTTTGCAGG+TGG + chr7_4:51831514-51831533 Msa1161660:intron 45.0%
AAGCATGTGCAGTATGAGCA+GGG - chr7_4:51831613-51831632 None:intergenic 45.0%
AAAGCATGTGCAGTATGAGC+AGG - chr7_4:51831614-51831633 None:intergenic 45.0%
TGACATTGCGTTGCTGCTAC+TGG - chr7_4:51829706-51829725 None:intergenic 50.0%
! TTGAAACATGGCGGAAGTGG+AGG - chr7_4:51829790-51829809 None:intergenic 50.0%
CCACTTCCGCCATGTTTCAA+CGG + chr7_4:51829790-51829809 Msa1161660:CDS 50.0%
GGAGAGAGTGAGGTCTTTGA+AGG - chr7_4:51829842-51829861 None:intergenic 50.0%
GGAAGATGGAGAGGACGAAA+GGG - chr7_4:51829863-51829882 None:intergenic 50.0%
AGGAAGATGGAGAGGACGAA+AGG - chr7_4:51829864-51829883 None:intergenic 50.0%
! AGCTCTAACGTCGTCGTTTG+GGG - chr7_4:51829946-51829965 None:intergenic 50.0%
TGGCAACAGGAGAAGGAAAG+CGG - chr7_4:51829989-51830008 None:intergenic 50.0%
CTTTCCTTCTCCTGTTGCCA+AGG + chr7_4:51829989-51830008 Msa1161660:CDS 50.0%
CCACGATTATCCTTGGCAAC+AGG - chr7_4:51830002-51830021 None:intergenic 50.0%
CCTGTTGCCAAGGATAATCG+TGG + chr7_4:51829999-51830018 Msa1161660:CDS 50.0%
AACAGCTCCACCTGAAATGC+TGG - chr7_4:51830060-51830079 None:intergenic 50.0%
ATTCGACCTGGGAAGTTGAG+AGG + chr7_4:51830107-51830126 Msa1161660:CDS 50.0%
!! GGGGTGCTGCTATCAAGTTT+AGG + chr7_4:51830171-51830190 Msa1161660:CDS 50.0%
TGGGTCATGCGCACAAGTTA+AGG - chr7_4:51830667-51830686 None:intergenic 50.0%
TTTGCAGGTGGAAAACAGCG+AGG + chr7_4:51831526-51831545 Msa1161660:intron 50.0%
GGCTATGCTGAGGTGATCAT+TGG + chr7_4:51831560-51831579 Msa1161660:CDS 50.0%
GGTGATCATTGGCAGAGATG+AGG + chr7_4:51831571-51831590 Msa1161660:CDS 50.0%
!! GTGGTAGTGGTGGTTCTCTC+TGG - chr7_4:51829681-51829700 None:intergenic 55.0%
CATTGCGTTGCTGCTACTGG+TGG - chr7_4:51829703-51829722 None:intergenic 55.0%
! AAACATGGCGGAAGTGGAGG+AGG - chr7_4:51829787-51829806 None:intergenic 55.0%
! CCGTTGAAACATGGCGGAAG+TGG - chr7_4:51829793-51829812 None:intergenic 55.0%
GCGGATCCGTTGAAACATGG+CGG - chr7_4:51829799-51829818 None:intergenic 55.0%
AGGAATGAGGTGACGAGGGA+GGG - chr7_4:51829822-51829841 None:intergenic 55.0%
AAGGAATGAGGTGACGAGGG+AGG - chr7_4:51829823-51829842 None:intergenic 55.0%
AGGACGAAAGGGAGAGAGTG+AGG - chr7_4:51829852-51829871 None:intergenic 55.0%
CGAGGAAGAGGAAGATGGAG+AGG - chr7_4:51829872-51829891 None:intergenic 55.0%
CCAAGCGAGGAAGAGGAAGA+TGG - chr7_4:51829877-51829896 None:intergenic 55.0%
CCATCTTCCTCTTCCTCGCT+TGG + chr7_4:51829874-51829893 Msa1161660:CDS 55.0%
GAGAGATCCAAGCGAGGAAG+AGG - chr7_4:51829884-51829903 None:intergenic 55.0%
AACGGAGAGAGATCCAAGCG+AGG - chr7_4:51829890-51829909 None:intergenic 55.0%
TTTGGGGAAGAGAGAGGGAG+TGG - chr7_4:51829930-51829949 None:intergenic 55.0%
CGTCGTTTGGGGAAGAGAGA+GGG - chr7_4:51829935-51829954 None:intergenic 55.0%
TCGTCGTTTGGGGAAGAGAG+AGG - chr7_4:51829936-51829955 None:intergenic 55.0%
GGCAACAGGAGAAGGAAAGC+GGG - chr7_4:51829988-51830007 None:intergenic 55.0%
!! GCTACTGGTGGTGGTAGTGG+TGG - chr7_4:51829691-51829710 None:intergenic 60.0%
!! GCTGCTACTGGTGGTGGTAG+TGG - chr7_4:51829694-51829713 None:intergenic 60.0%
TGCGTTGCTGCTACTGGTGG+TGG - chr7_4:51829700-51829719 None:intergenic 60.0%
! GGAGGAGGAGGATGGTGGTA+TGG - chr7_4:51829769-51829788 None:intergenic 60.0%
GAAGTGGAGGAGGAGGAGGA+TGG - chr7_4:51829777-51829796 None:intergenic 60.0%
GGGGCGGATCCGTTGAAACA+TGG - chr7_4:51829802-51829821 None:intergenic 60.0%
GGAATGAGGTGACGAGGGAG+GGG - chr7_4:51829821-51829840 None:intergenic 60.0%
!! TGGTCTTCGCGGTGTTGCGC+CGG - chr7_4:51829736-51829755 None:intergenic 65.0%
GTGGAGGAGGAGGAGGATGG+TGG - chr7_4:51829774-51829793 None:intergenic 65.0%
! CATGGCGGAAGTGGAGGAGG+AGG - chr7_4:51829784-51829803 None:intergenic 65.0%
ATGAGGTGACGAGGGAGGGG+CGG - chr7_4:51829818-51829837 None:intergenic 65.0%
GGCGGAAGTGGAGGAGGAGG+AGG - chr7_4:51829781-51829800 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 51829571 51832086 51829571 ID=Msa1161660;Name=Msa1161660
chr7_4 mRNA 51829571 51832086 51829571 ID=Msa1161660-mRNA-1;Parent=Msa1161660;Name=Msa1161660-mRNA-1;_AED=0.01;_eAED=0.01;_QI=95|1|1|1|1|1|2|347|244
chr7_4 exon 51829571 51830193 51829571 ID=Msa1161660-mRNA-1:exon:14758;Parent=Msa1161660-mRNA-1
chr7_4 exon 51831533 51832086 51831533 ID=Msa1161660-mRNA-1:exon:14759;Parent=Msa1161660-mRNA-1
chr7_4 five_prime_UTR 51829571 51829665 51829571 ID=Msa1161660-mRNA-1:five_prime_utr;Parent=Msa1161660-mRNA-1
chr7_4 CDS 51829666 51830193 51829666 ID=Msa1161660-mRNA-1:cds;Parent=Msa1161660-mRNA-1
chr7_4 CDS 51831533 51831739 51831533 ID=Msa1161660-mRNA-1:cds;Parent=Msa1161660-mRNA-1
chr7_4 three_prime_UTR 51831740 51832086 51831740 ID=Msa1161660-mRNA-1:three_prime_utr;Parent=Msa1161660-mRNA-1
Gene Sequence

>Msa1161660

ATGAGAGTGCAACCAGAGAGAACCACCACTACCACCACCAGTAGCAGCAACGCAATGTCATCAACAACCGGCGCAACACCGCGAAGACCAATCCAAATCACCATACCACCATCCTCCTCCTCCTCCACTTCCGCCATGTTTCAACGGATCCGCCCCTCCCTCGTCACCTCATTCCTTCAAAGACCTCACTCTCTCCCTTTCGTCCTCTCCATCTTCCTCTTCCTCGCTTGGATCTCTCTCCGTTCATATCGCTCTTCTCATCCACTCCCTCTCTCTTCCCCAAACGACGACGTTAGAGCTAATCTTCGAAGATTCTCTTCCCGCTTTCCTTCTCCTGTTGCCAAGGATAATCGTGGTTGGATTCTTGATCCTATTGCTCTCGCACTTTCTTCCAGCATTTCAGGTGGAGCTGTTACTTGTGCTTCTCTTCATCTTGGTGAAATTCGACCTGGGAAGTTGAGAGGAAATCATAGACATCATGATTGTAATGAGACTTTTGTTCTTTGGGGTGCTGCTATCAAGTTTAGGGTGGAAAACAGCGAGGTAACTGATATTGGCTATGCTGAGGTGATCATTGGCAGAGATGAGGTTGTTGTTGCTGTTAGCCCTGCTCATACTGCACATGCTTTAGTGAACATAGATCCGGTTCGGAGTGCTTACTTTATAGGATGCCAAGATAGTATCATAAACTATAATGCCTCAAGTACGGACTTTAATGTTTGGAAAGATCTTTAA
Protein sequence

>Msa1161660

MRVQPERTTTTTTSSSNAMSSTTGATPRRPIQITIPPSSSSSTSAMFQRIRPSLVTSFLQRPHSLPFVLSIFLFLAWISLRSYRSSHPLPLSSPNDDVRANLRRFSSRFPSPVAKDNRGWILDPIALALSSSISGGAVTCASLHLGEIRPGKLRGNHRHHDCNETFVLWGAAIKFRVENSEVTDIGYAEVIIGRDEVVVAVSPAHTAHALVNIDPVRSAYFIGCQDSIINYNASSTDFNVWKDL*