AlfalfaGEDB | Msa1161660

M. sativa cultivar zhongmu-4 / Msa1161660

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1161660	XP_003624824.2	93.886	229	11	1	19	244	1	229	3.71e-143	410

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1161660	A2Q1Y8	93.886	229	11	1	19	244	1	229	1.77e-143	410

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1072530	Msa1161660	0.810649	1.037563e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1161660	MtrunA17_Chr7g0254951	93.182	220	12	1	19	235	1	220	1.34e-138	389

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1161660	AT3G56820.1	55.140	214	74	5	48	244	12	220	6.64e-72	219
Msa1161660	AT3G56820.3	51.402	214	66	6	48	244	12	204	1.60e-60	189
Msa1161660	AT3G56820.2	50.340	147	53	3	48	177	12	155	2.89e-38	131

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Find 95 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAGACGGAATGAAA+GGG	0.482053	7_4:+51831921	Msa1161660:three_prime_UTR
AAACATGGCGGAAGTGGAGG+AGG	0.531130	7_4:-51829784	None:intergenic
AAACTATAATGCCTCAAGTA+CGG	0.551251	7_4:+51831691	Msa1161660:CDS
AAAGAAAGACGGAATGAAAG+GGG	0.614950	7_4:+51831922	Msa1161660:three_prime_UTR
AAAGCATGTGCAGTATGAGC+AGG	0.498771	7_4:-51831611	None:intergenic
AACAGCGAGGTAACTGATAT+TGG	0.393984	7_4:+51831539	Msa1161660:CDS
AACAGCTCCACCTGAAATGC+TGG	0.433121	7_4:-51830057	None:intergenic
AACATTAAAGTCCGTACTTG+AGG	0.548423	7_4:-51831702	None:intergenic
AACGCAATGTCATCAACAAC+CGG	0.502607	7_4:+51829714	Msa1161660:CDS
AACGGAGAGAGATCCAAGCG+AGG	0.715907	7_4:-51829887	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGGTTCCTCTAATTCAATT+TGG	+	chr7_4:51830308-51830327	Msa1161660:intron	30.0%
TACACTCCAAATTGAATTAG+AGG	-	chr7_4:51830317-51830336	None:intergenic	30.0%
ACAGCCTAACATAATAGTTA+AGG	-	chr7_4:51830521-51830540	None:intergenic	30.0%
ATTGTGTTTGATGTTATACC+GGG	+	chr7_4:51830590-51830609	Msa1161660:intron	30.0%
CATTAATAGATCCTAGTTAC+AGG	-	chr7_4:51830756-51830775	None:intergenic	30.0%
AAGATGAAAATTACGCTCTA+TGG	+	chr7_4:51830799-51830818	Msa1161660:intron	30.0%
AATACTTATTGATCCAATGG+TGG	-	chr7_4:51830948-51830967	None:intergenic	30.0%
ACATATCACATTTGATCCAA+TGG	-	chr7_4:51830972-51830991	None:intergenic	30.0%
GTTGTTATGCAAAATGAAGT+TGG	-	chr7_4:51831098-51831117	None:intergenic	30.0%
TTAAAATGGAAACAAAGGGA+AGG	-	chr7_4:51831120-51831139	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	51829571	51832086	51829571	ID=Msa1161660;Name=Msa1161660
chr7_4	mRNA	51829571	51832086	51829571	ID=Msa1161660-mRNA-1;Parent=Msa1161660;Name=Msa1161660-mRNA-1;_AED=0.01;_eAED=0.01;_QI=95\|1\|1\|1\|1\|1\|2\|347\|244
chr7_4	exon	51829571	51830193	51829571	ID=Msa1161660-mRNA-1:exon:14758;Parent=Msa1161660-mRNA-1
chr7_4	exon	51831533	51832086	51831533	ID=Msa1161660-mRNA-1:exon:14759;Parent=Msa1161660-mRNA-1
chr7_4	five_prime_UTR	51829571	51829665	51829571	ID=Msa1161660-mRNA-1:five_prime_utr;Parent=Msa1161660-mRNA-1
chr7_4	CDS	51829666	51830193	51829666	ID=Msa1161660-mRNA-1:cds;Parent=Msa1161660-mRNA-1
chr7_4	CDS	51831533	51831739	51831533	ID=Msa1161660-mRNA-1:cds;Parent=Msa1161660-mRNA-1
chr7_4	three_prime_UTR	51831740	51832086	51831740	ID=Msa1161660-mRNA-1:three_prime_utr;Parent=Msa1161660-mRNA-1

Gene Sequence

>Msa1161660

ATGAGAGTGCAACCAGAGAGAACCACCACTACCACCACCAGTAGCAGCAACGCAATGTCATCAACAACCGGCGCAACACCGCGAAGACCAATCCAAATCACCATACCACCATCCTCCTCCTCCTCCACTTCCGCCATGTTTCAACGGATCCGCCCCTCCCTCGTCACCTCATTCCTTCAAAGACCTCACTCTCTCCCTTTCGTCCTCTCCATCTTCCTCTTCCTCGCTTGGATCTCTCTCCGTTCATATCGCTCTTCTCATCCACTCCCTCTCTCTTCCCCAAACGACGACGTTAGAGCTAATCTTCGAAGATTCTCTTCCCGCTTTCCTTCTCCTGTTGCCAAGGATAATCGTGGTTGGATTCTTGATCCTATTGCTCTCGCACTTTCTTCCAGCATTTCAGGTGGAGCTGTTACTTGTGCTTCTCTTCATCTTGGTGAAATTCGACCTGGGAAGTTGAGAGGAAATCATAGACATCATGATTGTAATGAGACTTTTGTTCTTTGGGGTGCTGCTATCAAGTTTAGGGTGGAAAACAGCGAGGTAACTGATATTGGCTATGCTGAGGTGATCATTGGCAGAGATGAGGTTGTTGTTGCTGTTAGCCCTGCTCATACTGCACATGCTTTAGTGAACATAGATCCGGTTCGGAGTGCTTACTTTATAGGATGCCAAGATAGTATCATAAACTATAATGCCTCAAGTACGGACTTTAATGTTTGGAAAGATCTTTAA

Protein sequence

>Msa1161660

MRVQPERTTTTTTSSSNAMSSTTGATPRRPIQITIPPSSSSSTSAMFQRIRPSLVTSFLQRPHSLPFVLSIFLFLAWISLRSYRSSHPLPLSSPNDDVRANLRRFSSRFPSPVAKDNRGWILDPIALALSSSISGGAVTCASLHLGEIRPGKLRGNHRHHDCNETFVLWGAAIKFRVENSEVTDIGYAEVIIGRDEVVVAVSPAHTAHALVNIDPVRSAYFIGCQDSIINYNASSTDFNVWKDL*