AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1162010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1162010 MtrunA17_Chr7g0257681 97.015 268 8 0 1 268 1 268 0.0 538
Msa1162010 MtrunA17_Chr1g0173421 67.247 287 73 4 1 268 1 285 7.02e-133 377
Msa1162010 MtrunA17_Chr6g0451591 42.675 157 82 3 2 154 50 202 3.09e-36 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1162010 AT2G41835.1 58.803 284 94 5 1 268 1 277 4.66e-112 324
Msa1162010 AT3G57480.2 58.209 268 91 4 1 268 1 247 1.41e-103 301
Msa1162010 AT3G57480.1 58.209 268 91 4 1 268 1 247 1.41e-103 301
Msa1162010 AT3G28210.2 44.872 156 77 4 2 154 5 154 7.55e-36 127
Msa1162010 AT3G28210.1 44.872 156 77 4 2 154 5 154 7.55e-36 127
Msa1162010 AT5G48205.1 55.046 109 21 3 1 106 1 84 9.91e-29 108

Find 103 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCCCATTTCTGGATTTC+TGG -0.035419 7_4:-52389070 Msa1162010:five_prime_UTR
AGACGATTCCCTCCCATTTC+TGG 0.113556 7_4:-52389078 Msa1162010:five_prime_UTR
GTTCATTAAATAATTTATTT+TGG 0.197602 7_4:+52389150 None:intergenic
TGACGTGGTTGAAGTTAAAT+TGG 0.210618 7_4:+52387223 None:intergenic
AATTATTAGAGCGTGGAATT+AGG 0.225673 7_4:-52389105 Msa1162010:five_prime_UTR
TTGGATCTTGGTCTGGAATT+AGG 0.236801 7_4:+52387510 None:intergenic
TAATTAGAGAATCATAATTT+TGG 0.240465 7_4:+52389128 None:intergenic
AATGAATAGCACTTTCTTGA+TGG 0.275542 7_4:-52386883 Msa1162010:three_prime_UTR
AGGAGTGGCGGCAACCAATA+TGG 0.284572 7_4:-52387035 Msa1162010:CDS
ACCAAGATCCAAACATAGTT+TGG 0.297216 7_4:-52387499 Msa1162010:CDS
TTCTGGATTTCTGGTTGGTT+GGG 0.311039 7_4:-52389061 Msa1162010:five_prime_UTR
CTTGCCTAGATCTGGGAATT+CGG 0.320931 7_4:+52388881 None:intergenic
ACATGCTCTACAAGGGAAAC+TGG 0.328561 7_4:+52386957 None:intergenic
TTTGAGCTTGGTTTGGATCA+TGG 0.341751 7_4:-52388910 Msa1162010:exon
ATTCTACTACTCGTTGTCTT+TGG 0.343219 7_4:-52389184 Msa1162010:five_prime_UTR
ATTATTAGAGCGTGGAATTA+GGG 0.343452 7_4:-52389104 Msa1162010:five_prime_UTR
CCCAAACTATGTTTGGATCT+TGG 0.346442 7_4:+52387498 None:intergenic
TCTTCGGTTTGAGCTTGGTT+TGG 0.348637 7_4:-52388917 Msa1162010:five_prime_UTR
GACTTCAAGCCTTCTTGACT+TGG 0.353989 7_4:-52387192 Msa1162010:CDS
CACTTGTGATCGTTGCAATC+AGG 0.365381 7_4:-52388814 Msa1162010:intron
CCCATTTCTGGATTTCTGGT+TGG 0.366874 7_4:-52389066 Msa1162010:five_prime_UTR
TTCATACATACACCGATATA+CGG 0.368726 7_4:-52386722 Msa1162010:three_prime_UTR
GAGCAATGCTTGCCTAGATC+TGG 0.369502 7_4:+52388873 None:intergenic
AACGGTGACATCTTGTTTGT+TGG 0.370225 7_4:+52387565 None:intergenic
AGGATTTCGTTTCTTCTCTT+CGG 0.372070 7_4:-52388933 Msa1162010:five_prime_UTR
ACACCTTCTTTGTCCCATAT+TGG 0.377110 7_4:+52387021 None:intergenic
TATGTCCACTTTGTGCTAAA+GGG 0.378295 7_4:-52387538 Msa1162010:CDS
TTTCTGGATTTCTGGTTGGT+TGG 0.383815 7_4:-52389062 Msa1162010:five_prime_UTR
CAGAAAACCTTTACTGCACT+TGG 0.385251 7_4:+52386983 None:intergenic
GGTCCAGGACATTTGTGATC+AGG 0.386396 7_4:+52387317 None:intergenic
TTGTGAATTACGCGGATATA+GGG 0.387535 7_4:+52389022 None:intergenic
TTGAGCTTGGTTTGGATCAT+GGG 0.395085 7_4:-52388909 Msa1162010:exon
ACCAACCAGAAATCCAGAAA+TGG 0.401843 7_4:+52389065 None:intergenic
TGACGCTCCCAAACTATGTT+TGG 0.404477 7_4:+52387491 None:intergenic
CATTGTTTGAAGCACAGGTT+TGG 0.404986 7_4:-52387341 Msa1162010:CDS
ACTAAGGTCTCTCTGCATCC+TGG 0.410212 7_4:+52387404 None:intergenic
ATATGTCCACTTTGTGCTAA+AGG 0.412254 7_4:-52387539 Msa1162010:CDS
TTGACCATTGTTTGAAGCAC+AGG 0.415680 7_4:-52387346 Msa1162010:CDS
TTGCCTAGATCTGGGAATTC+GGG 0.416402 7_4:+52388882 None:intergenic
TACTCGTTGTCTTTGGCAAA+TGG 0.417251 7_4:-52389177 Msa1162010:five_prime_UTR
ATATTAGATTTATTTGATCA+TGG 0.419688 7_4:-52386788 Msa1162010:three_prime_UTR
AGAAGAATCAATCCGTATAT+CGG 0.427532 7_4:+52386710 None:intergenic
TCGGTGTATGTATGAATTGT+AGG 0.428418 7_4:+52386729 None:intergenic
CTAAGGTCTCTCTGCATCCT+GGG 0.434135 7_4:+52387405 None:intergenic
GACGTGGTTGAAGTTAAATT+GGG 0.435317 7_4:+52387224 None:intergenic
ACTATGTTTGGATCTTGGTC+TGG 0.435501 7_4:+52387503 None:intergenic
ACGGTGACATCTTGTTTGTT+GGG 0.438220 7_4:+52387566 None:intergenic
TCTTCTCTTCGGTTTGAGCT+TGG 0.443261 7_4:-52388922 Msa1162010:five_prime_UTR
CCCTCTCAACATGCTCTACA+AGG 0.443451 7_4:+52386949 None:intergenic
ATTGTGAATTACGCGGATAT+AGG 0.451113 7_4:+52389021 None:intergenic
GGAGTGGCGGCAACCAATAT+GGG 0.453616 7_4:-52387034 Msa1162010:CDS
CCAAGATCCAAACATAGTTT+GGG 0.458482 7_4:-52387498 Msa1162010:CDS
GTCTCTCTGCATCCTGGGGC+AGG 0.461132 7_4:+52387410 None:intergenic
CTTCTTTGTTGTAATGCTGT+TGG 0.468468 7_4:-52386692 Msa1162010:three_prime_UTR
TGGTACTAGTAATAATCAAC+TGG 0.476635 7_4:-52387132 Msa1162010:CDS
GATTGTGTTTGAGAATACTA+AGG 0.477004 7_4:+52387388 None:intergenic
GCATGCCCCAAGTGCAGTAA+AGG 0.479086 7_4:-52386990 Msa1162010:CDS
TATTAGATTTATTTGATCAT+GGG 0.481987 7_4:-52386787 Msa1162010:three_prime_UTR
GCTTCTAAATTGAGTGGGAT+AGG 0.483203 7_4:-52387170 Msa1162010:CDS
GTTGTTCTTGAGAATTATTG+TGG 0.500974 7_4:-52386917 Msa1162010:exon
CCAACCAGAAATCCAGAAAT+GGG 0.503012 7_4:+52389066 None:intergenic
ATCCACCAAACTCGTGGCCG+AGG 0.511045 7_4:+52387079 None:intergenic
TTCCTCGGCCACGAGTTTGG+TGG 0.518637 7_4:-52387081 Msa1162010:CDS
CTCAAACACAATCAAGTGTA+GGG 0.519840 7_4:-52387378 Msa1162010:CDS
ATTTAGAAGCCAAGTCAAGA+AGG 0.521688 7_4:+52387183 None:intergenic
GGACCTGATCACAAATGTCC+TGG 0.523810 7_4:-52387320 Msa1162010:CDS
AGAAAACCTTTACTGCACTT+GGG 0.532761 7_4:+52386984 None:intergenic
CATGCTCTACAAGGGAAACT+GGG 0.533711 7_4:+52386958 None:intergenic
ACTTGGCTTCTAAATTGAGT+GGG 0.535363 7_4:-52387175 Msa1162010:CDS
TGAGCTTGGTTTGGATCATG+GGG 0.539843 7_4:-52388908 Msa1162010:exon
CTTCAACCACGTCATCTAAG+TGG 0.540578 7_4:-52387214 Msa1162010:CDS
CCTTGTAGAGCATGTTGAGA+GGG 0.543468 7_4:-52386949 Msa1162010:CDS
ACTCCCGAATTCCCAGATCT+AGG 0.548420 7_4:-52388885 Msa1162010:CDS
GACTTGGCTTCTAAATTGAG+TGG 0.550800 7_4:-52387176 Msa1162010:CDS
AGCAATGCTTGCCTAGATCT+GGG 0.554462 7_4:+52388874 None:intergenic
CAAACCTGTGCTTCAAACAA+TGG 0.566927 7_4:+52387342 None:intergenic
GAGCATGTTGAGAGGGATCA+TGG 0.569049 7_4:-52386942 Msa1162010:CDS
ATGTCCACTTTGTGCTAAAG+GGG 0.570588 7_4:-52387537 Msa1162010:CDS
GAAAACCTTTACTGCACTTG+GGG 0.571579 7_4:+52386985 None:intergenic
TCTCAAACACAATCAAGTGT+AGG 0.571728 7_4:-52387379 Msa1162010:CDS
ATTAGAGCGTGGAATTAGGG+AGG 0.572050 7_4:-52389101 Msa1162010:five_prime_UTR
CTAATTAAATTATTAGAGCG+TGG 0.572217 7_4:-52389112 Msa1162010:five_prime_UTR
ATAGGACAGAGCAGTGGCAG+TGG 0.577401 7_4:-52387152 Msa1162010:CDS
CGCATGATCCACCAAACTCG+TGG 0.584791 7_4:+52387073 None:intergenic
ATTGCAACGATCACAAGTGA+AGG 0.586850 7_4:+52388818 None:intergenic
CAACCAATATGGGACAAAGA+AGG 0.587060 7_4:-52387024 Msa1162010:CDS
AGTCTTTGGAATATGAGTAA+TGG 0.594698 7_4:-52387263 Msa1162010:CDS
AATTATGCTCAATATAACTT+CGG 0.599455 7_4:+52387597 None:intergenic
CCCTTGTAGAGCATGTTGAG+AGG 0.611040 7_4:-52386950 Msa1162010:CDS
CCAGAAATCCAGAAATGGGA+GGG 0.613406 7_4:+52389070 None:intergenic
CGAACCCCTTTAGCACAAAG+TGG 0.621156 7_4:+52387533 None:intergenic
GAAAACTTAGCATCGCATAG+TGG 0.630859 7_4:+52387101 None:intergenic
AAAGTTCATCAAAGGAGTGG+CGG 0.638449 7_4:-52387047 Msa1162010:CDS
ACCAGAAATCCAGAAATGGG+AGG 0.639433 7_4:+52389069 None:intergenic
TGAAGTCCACTTAGATGACG+TGG 0.643218 7_4:+52387208 None:intergenic
TTGCAACGATCACAAGTGAA+GGG 0.653221 7_4:+52388819 None:intergenic
CCTCTCAACATGCTCTACAA+GGG 0.654661 7_4:+52386950 None:intergenic
TAAGGTCTCTCTGCATCCTG+GGG 0.659577 7_4:+52387406 None:intergenic
AATCAAGTGTAGGGACTGTG+AGG 0.678787 7_4:-52387369 Msa1162010:CDS
AGTGGGATAGGACAGAGCAG+TGG 0.703021 7_4:-52387158 Msa1162010:CDS
GTGGAGAATTGTGAATTACG+CGG 0.729104 7_4:+52389014 None:intergenic
ACAAAGTGGACATATAACAA+CGG 0.739755 7_4:+52387547 None:intergenic
CATGTTGAGAGGGATCATGG+TGG 0.808965 7_4:-52386939 Msa1162010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTCATTAAATAATTTATTT+TGG + chr7_4:52386777-52386796 None:intergenic 10.0%
!!! GTTATTTTCATAAATTATTT+TGG - chr7_4:52388067-52388086 Msa1162010:intron 10.0%
!!! TAATTAGAGAATCATAATTT+TGG + chr7_4:52386799-52386818 None:intergenic 15.0%
!! AAAAAAAAATAAATGAGGAA+TGG + chr7_4:52386966-52386985 None:intergenic 15.0%
!! TTTGTTTCGAAATTAATTAA+TGG - chr7_4:52387157-52387176 Msa1162010:CDS 15.0%
!! TTGTTTCGAAATTAATTAAT+GGG - chr7_4:52387158-52387177 Msa1162010:CDS 15.0%
!! TTGTATATATATATTGGTAT+TGG - chr7_4:52387651-52387670 Msa1162010:intron 15.0%
!!! TATCAGTTTATTTTAGAATA+AGG - chr7_4:52387676-52387695 Msa1162010:intron 15.0%
!! ATATTAGATTTATTTGATCA+TGG - chr7_4:52389136-52389155 Msa1162010:five_prime_UTR 15.0%
!! TATTAGATTTATTTGATCAT+GGG - chr7_4:52389137-52389156 Msa1162010:five_prime_UTR 15.0%
!!! TCCTCATTTATTTTTTTTTC+TGG - chr7_4:52386967-52386986 Msa1162010:CDS 20.0%
!! TCCAGAAAAAAAAATAAATG+AGG + chr7_4:52386971-52386990 None:intergenic 20.0%
!!! CATTTATTTTTTTTTCTGGA+AGG - chr7_4:52386971-52386990 Msa1162010:CDS 20.0%
!! AGAAAATAATAGAATGAAAC+GGG + chr7_4:52387208-52387227 None:intergenic 20.0%
!! GAGAAAATAATAGAATGAAA+CGG + chr7_4:52387209-52387228 None:intergenic 20.0%
!!! TTTCATTCTATTATTTTCTC+CGG - chr7_4:52387209-52387228 Msa1162010:CDS 20.0%
!!! GAAACAATGATTTTAATCAA+AGG + chr7_4:52387268-52387287 None:intergenic 20.0%
!!! AGGATTTTTTCAAAATGAAA+TGG - chr7_4:52387696-52387715 Msa1162010:intron 20.0%
!! TGATTATTTCATTTGAGAAA+TGG - chr7_4:52387764-52387783 Msa1162010:intron 20.0%
!! TGAATTTATTGAAATAAGCT+AGG - chr7_4:52388090-52388109 Msa1162010:intron 20.0%
!! AATCACTTACATAAAATGTT+TGG + chr7_4:52388135-52388154 None:intergenic 20.0%
!! AATTATGCTCAATATAACTT+CGG + chr7_4:52388330-52388349 None:intergenic 20.0%
!!! CAATATTTTGCTATCAAATA+GGG + chr7_4:52389094-52389113 None:intergenic 20.0%
!!! ACAATATTTTGCTATCAAAT+AGG + chr7_4:52389095-52389114 None:intergenic 20.0%
! CTAATTAAATTATTAGAGCG+TGG - chr7_4:52386812-52386831 Msa1162010:three_prime_UTR 25.0%
!! TTCATTCTATTATTTTCTCC+GGG - chr7_4:52387210-52387229 Msa1162010:CDS 25.0%
!! TATTGATTATAGCAATAACC+CGG + chr7_4:52387231-52387250 None:intergenic 25.0%
! CACAATTTAATCCAACAATA+TGG + chr7_4:52387362-52387381 None:intergenic 25.0%
! TATTGTTGGATTAAATTGTG+TGG - chr7_4:52387362-52387381 Msa1162010:CDS 25.0%
! GCTGGATTGTATATATATAT+TGG - chr7_4:52387645-52387664 Msa1162010:intron 25.0%
! GTTTCACAAAACTATAGTAA+TGG + chr7_4:52387728-52387747 None:intergenic 25.0%
! ACATAAAGTAAATCATTTGC+TGG - chr7_4:52387806-52387825 Msa1162010:intron 25.0%
!! TATCATCTATATAGAGTGTT+TGG - chr7_4:52387934-52387953 Msa1162010:intron 25.0%
! GTAACTTAATTAAGTGCTTA+CGG - chr7_4:52387961-52387980 Msa1162010:intron 25.0%
! ATTTATTGAAATAAGCTAGG+AGG - chr7_4:52388093-52388112 Msa1162010:intron 25.0%
!!! AAACTTGTTTCCAACTTTTT+GGG + chr7_4:52388631-52388650 None:intergenic 25.0%
!!! AAAACTTGTTTCCAACTTTT+TGG + chr7_4:52388632-52388651 None:intergenic 25.0%
!! GTTTTTCATTTATGAGTCTT+TGG - chr7_4:52388647-52388666 Msa1162010:intron 25.0%
AATTATTAGAGCGTGGAATT+AGG - chr7_4:52386819-52386838 Msa1162010:three_prime_UTR 30.0%
ATTATTAGAGCGTGGAATTA+GGG - chr7_4:52386820-52386839 Msa1162010:three_prime_UTR 30.0%
!!! ATTTGTTTTGTTCGAATCCT+TGG - chr7_4:52387317-52387336 Msa1162010:CDS 30.0%
TCCAACAATATGGACATTAT+CGG + chr7_4:52387352-52387371 None:intergenic 30.0%
! CGTTTAAGTGTTTGACTTAT+AGG - chr7_4:52387446-52387465 Msa1162010:CDS 30.0%
! TTAAGTGTTTGACTTATAGG+AGG - chr7_4:52387449-52387468 Msa1162010:CDS 30.0%
!!! TGTTAGTTTTTCCTGTTGTT+AGG + chr7_4:52387540-52387559 None:intergenic 30.0%
! ATAGAATTGACTTTGAAAGC+TGG - chr7_4:52387627-52387646 Msa1162010:intron 30.0%
CTTCACTGAAGTTTCTATTA+AGG - chr7_4:52387870-52387889 Msa1162010:intron 30.0%
TTGGAAATATGTAGTTGTGA+TGG - chr7_4:52388217-52388236 Msa1162010:intron 30.0%
AGTTGCTTAGTTGAAAACAA+GGG + chr7_4:52388251-52388270 None:intergenic 30.0%
AACAATATACCTGCAAAAGA+TGG + chr7_4:52388300-52388319 None:intergenic 30.0%
! TCTTTTGCAGGTATATTGTT+TGG - chr7_4:52388300-52388319 Msa1162010:intron 30.0%
! CTTTTGCAGGTATATTGTTT+GGG - chr7_4:52388301-52388320 Msa1162010:intron 30.0%
! TTTTGCAGGTATATTGTTTG+GGG - chr7_4:52388302-52388321 Msa1162010:intron 30.0%
ACAAAGTGGACATATAACAA+CGG + chr7_4:52388380-52388399 None:intergenic 30.0%
!!! GTGGCTTTTTCATAATTTGA+TGG + chr7_4:52388478-52388497 None:intergenic 30.0%
GATTGTGTTTGAGAATACTA+AGG + chr7_4:52388539-52388558 None:intergenic 30.0%
!! AGTCTTTGGAATATGAGTAA+TGG - chr7_4:52388661-52388680 Msa1162010:intron 30.0%
TGGTACTAGTAATAATCAAC+TGG - chr7_4:52388792-52388811 Msa1162010:intron 30.0%
ATGCGAAAAAAGTTCATCAA+AGG - chr7_4:52388869-52388888 Msa1162010:CDS 30.0%
AAAAAAGTTCATCAAAGGAG+TGG - chr7_4:52388874-52388893 Msa1162010:CDS 30.0%
GTTGTTCTTGAGAATTATTG+TGG - chr7_4:52389007-52389026 Msa1162010:five_prime_UTR 30.0%
AATGAATAGCACTTTCTTGA+TGG - chr7_4:52389041-52389060 Msa1162010:five_prime_UTR 30.0%
GCTCGCAAATAACAAAAAAT+AGG + chr7_4:52389176-52389195 None:intergenic 30.0%
TTCATACATACACCGATATA+CGG - chr7_4:52389202-52389221 Msa1162010:five_prime_UTR 30.0%
AGAAGAATCAATCCGTATAT+CGG + chr7_4:52389217-52389236 None:intergenic 30.0%
ATTCTACTACTCGTTGTCTT+TGG - chr7_4:52386740-52386759 Msa1162010:three_prime_UTR 35.0%
TTGTGAATTACGCGGATATA+GGG + chr7_4:52386905-52386924 None:intergenic 35.0%
ATTGTGAATTACGCGGATAT+AGG + chr7_4:52386906-52386925 None:intergenic 35.0%
AGGATTTCGTTTCTTCTCTT+CGG - chr7_4:52386991-52387010 Msa1162010:CDS 35.0%
! ATTATCGGTTGAACTAACCA+AGG + chr7_4:52387337-52387356 None:intergenic 35.0%
ACCGATAATGTCCATATTGT+TGG - chr7_4:52387348-52387367 Msa1162010:CDS 35.0%
! TGTTTGACTTATAGGAGGTT+GGG - chr7_4:52387454-52387473 Msa1162010:CDS 35.0%
TTGCTGGAAATAATGAACTG+AGG - chr7_4:52387822-52387841 Msa1162010:intron 35.0%
AAGGTGAATGCTTAATGCAA+AGG - chr7_4:52387889-52387908 Msa1162010:intron 35.0%
!!! ATTTTGACTTCTCCAGTTCT+TGG - chr7_4:52388191-52388210 Msa1162010:intron 35.0%
!!! TTTTGACTTCTCCAGTTCTT+GGG - chr7_4:52388192-52388211 Msa1162010:intron 35.0%
GAGTTGCTTAGTTGAAAACA+AGG + chr7_4:52388252-52388271 None:intergenic 35.0%
AACTAAGCAACTCTTTGTGA+AGG - chr7_4:52388259-52388278 Msa1162010:intron 35.0%
ATATGTCCACTTTGTGCTAA+AGG - chr7_4:52388385-52388404 Msa1162010:intron 35.0%
TATGTCCACTTTGTGCTAAA+GGG - chr7_4:52388386-52388405 Msa1162010:intron 35.0%
ACCAAGATCCAAACATAGTT+TGG - chr7_4:52388425-52388444 Msa1162010:intron 35.0%
CCAAGATCCAAACATAGTTT+GGG - chr7_4:52388426-52388445 Msa1162010:intron 35.0%
!!! AGGACACTTTTTCTTCTTTG+TGG + chr7_4:52388497-52388516 None:intergenic 35.0%
TCTCAAACACAATCAAGTGT+AGG - chr7_4:52388545-52388564 Msa1162010:intron 35.0%
CTCAAACACAATCAAGTGTA+GGG - chr7_4:52388546-52388565 Msa1162010:intron 35.0%
GACGTGGTTGAAGTTAAATT+GGG + chr7_4:52388703-52388722 None:intergenic 35.0%
TGACGTGGTTGAAGTTAAAT+TGG + chr7_4:52388704-52388723 None:intergenic 35.0%
ATTTAGAAGCCAAGTCAAGA+AGG + chr7_4:52388744-52388763 None:intergenic 35.0%
ACTTGGCTTCTAAATTGAGT+GGG - chr7_4:52388749-52388768 Msa1162010:intron 35.0%
AGAAAACCTTTACTGCACTT+GGG + chr7_4:52388943-52388962 None:intergenic 35.0%
TCGGTGTATGTATGAATTGT+AGG + chr7_4:52389198-52389217 None:intergenic 35.0%
! CTTCTTTGTTGTAATGCTGT+TGG - chr7_4:52389232-52389251 Msa1162010:five_prime_UTR 35.0%
TACTCGTTGTCTTTGGCAAA+TGG - chr7_4:52386747-52386766 Msa1162010:three_prime_UTR 40.0%
CCAACCAGAAATCCAGAAAT+GGG + chr7_4:52386861-52386880 None:intergenic 40.0%
ACCAACCAGAAATCCAGAAA+TGG + chr7_4:52386862-52386881 None:intergenic 40.0%
!! TTTCTGGATTTCTGGTTGGT+TGG - chr7_4:52386862-52386881 Msa1162010:three_prime_UTR 40.0%
!! TTCTGGATTTCTGGTTGGTT+GGG - chr7_4:52386863-52386882 Msa1162010:three_prime_UTR 40.0%
GTGGAGAATTGTGAATTACG+CGG + chr7_4:52386913-52386932 None:intergenic 40.0%
!! TTTGAGCTTGGTTTGGATCA+TGG - chr7_4:52387014-52387033 Msa1162010:CDS 40.0%
!! TTGAGCTTGGTTTGGATCAT+GGG - chr7_4:52387015-52387034 Msa1162010:CDS 40.0%
TTGCAACGATCACAAGTGAA+GGG + chr7_4:52387108-52387127 None:intergenic 40.0%
ATTGCAACGATCACAAGTGA+AGG + chr7_4:52387109-52387128 None:intergenic 40.0%
! GTGTTTGACTTATAGGAGGT+TGG - chr7_4:52387453-52387472 Msa1162010:CDS 40.0%
AGTTCAACTAGTTCGAGCTA+AGG - chr7_4:52387478-52387497 Msa1162010:CDS 40.0%
GTTCAACTAGTTCGAGCTAA+GGG - chr7_4:52387479-52387498 Msa1162010:CDS 40.0%
AAGGGTTAAGAAGTTGGAGT+TGG - chr7_4:52387497-52387516 Msa1162010:CDS 40.0%
AGAGTTCAAGTCCTAACAAC+AGG - chr7_4:52387526-52387545 Msa1162010:CDS 40.0%
AAACCATGCAACAACCTTAC+TGG + chr7_4:52387915-52387934 None:intergenic 40.0%
! ATGTTTGGCTCATGTCCATA+GGG + chr7_4:52388120-52388139 None:intergenic 40.0%
! AATGTTTGGCTCATGTCCAT+AGG + chr7_4:52388121-52388140 None:intergenic 40.0%
! TTTGACTTCTCCAGTTCTTG+GGG - chr7_4:52388193-52388212 Msa1162010:intron 40.0%
TATTTCCAAACCCCAAGAAC+TGG + chr7_4:52388206-52388225 None:intergenic 40.0%
! ATATCAGCTCCATCTTTTGC+AGG - chr7_4:52388288-52388307 Msa1162010:intron 40.0%
! ACGGTGACATCTTGTTTGTT+GGG + chr7_4:52388361-52388380 None:intergenic 40.0%
! AACGGTGACATCTTGTTTGT+TGG + chr7_4:52388362-52388381 None:intergenic 40.0%
ATGTCCACTTTGTGCTAAAG+GGG - chr7_4:52388387-52388406 Msa1162010:intron 40.0%
! TTGGATCTTGGTCTGGAATT+AGG + chr7_4:52388417-52388436 None:intergenic 40.0%
ACTATGTTTGGATCTTGGTC+TGG + chr7_4:52388424-52388443 None:intergenic 40.0%
CCCAAACTATGTTTGGATCT+TGG + chr7_4:52388429-52388448 None:intergenic 40.0%
! TTGACCATTGTTTGAAGCAC+AGG - chr7_4:52388578-52388597 Msa1162010:intron 40.0%
CAAACCTGTGCTTCAAACAA+TGG + chr7_4:52388585-52388604 None:intergenic 40.0%
! CATTGTTTGAAGCACAGGTT+TGG - chr7_4:52388583-52388602 Msa1162010:intron 40.0%
GACTTGGCTTCTAAATTGAG+TGG - chr7_4:52388748-52388767 Msa1162010:intron 40.0%
GCTTCTAAATTGAGTGGGAT+AGG - chr7_4:52388754-52388773 Msa1162010:intron 40.0%
GAAAACTTAGCATCGCATAG+TGG + chr7_4:52388826-52388845 None:intergenic 40.0%
! ATGCGATGCTAAGTTTTCCT+CGG - chr7_4:52388828-52388847 Msa1162010:CDS 40.0%
AAAGTTCATCAAAGGAGTGG+CGG - chr7_4:52388877-52388896 Msa1162010:CDS 40.0%
CAACCAATATGGGACAAAGA+AGG - chr7_4:52388900-52388919 Msa1162010:exon 40.0%
ACACCTTCTTTGTCCCATAT+TGG + chr7_4:52388906-52388925 None:intergenic 40.0%
GAAAACCTTTACTGCACTTG+GGG + chr7_4:52388942-52388961 None:intergenic 40.0%
CAGAAAACCTTTACTGCACT+TGG + chr7_4:52388944-52388963 None:intergenic 40.0%
! CAAGTGCAGTAAAGGTTTTC+TGG - chr7_4:52388942-52388961 Msa1162010:five_prime_UTR 40.0%
ATTAGAGCGTGGAATTAGGG+AGG - chr7_4:52386823-52386842 Msa1162010:three_prime_UTR 45.0%
CCAGAAATCCAGAAATGGGA+GGG + chr7_4:52386857-52386876 None:intergenic 45.0%
ACCAGAAATCCAGAAATGGG+AGG + chr7_4:52386858-52386877 None:intergenic 45.0%
!! CCCATTTCTGGATTTCTGGT+TGG - chr7_4:52386858-52386877 Msa1162010:three_prime_UTR 45.0%
! TCTTCTCTTCGGTTTGAGCT+TGG - chr7_4:52387002-52387021 Msa1162010:CDS 45.0%
! TCTTCGGTTTGAGCTTGGTT+TGG - chr7_4:52387007-52387026 Msa1162010:CDS 45.0%
!! TGAGCTTGGTTTGGATCATG+GGG - chr7_4:52387016-52387035 Msa1162010:CDS 45.0%
TTGCCTAGATCTGGGAATTC+GGG + chr7_4:52387045-52387064 None:intergenic 45.0%
CTTGCCTAGATCTGGGAATT+CGG + chr7_4:52387046-52387065 None:intergenic 45.0%
AGCAATGCTTGCCTAGATCT+GGG + chr7_4:52387053-52387072 None:intergenic 45.0%
CACTTGTGATCGTTGCAATC+AGG - chr7_4:52387110-52387129 Msa1162010:CDS 45.0%
CGAGCTAAGGGTTAAGAAGT+TGG - chr7_4:52387491-52387510 Msa1162010:CDS 45.0%
GGTTAAGAAGTTGGAGTTGG+AGG - chr7_4:52387500-52387519 Msa1162010:CDS 45.0%
GCTTAATGCAAAGGCCAGTA+AGG - chr7_4:52387898-52387917 Msa1162010:intron 45.0%
AATAAGCTAGGAGGACCCTA+TGG - chr7_4:52388102-52388121 Msa1162010:intron 45.0%
TGACGCTCCCAAACTATGTT+TGG + chr7_4:52388436-52388455 None:intergenic 45.0%
AATCAAGTGTAGGGACTGTG+AGG - chr7_4:52388555-52388574 Msa1162010:intron 45.0%
ATGTCCTGGACCCAAAAAGT+TGG - chr7_4:52388618-52388637 Msa1162010:intron 45.0%
!!! GTTTCCAACTTTTTGGGTCC+AGG + chr7_4:52388625-52388644 None:intergenic 45.0%
CTTCAACCACGTCATCTAAG+TGG - chr7_4:52388710-52388729 Msa1162010:intron 45.0%
TGAAGTCCACTTAGATGACG+TGG + chr7_4:52388719-52388738 None:intergenic 45.0%
GACTTCAAGCCTTCTTGACT+TGG - chr7_4:52388732-52388751 Msa1162010:intron 45.0%
CATGCTCTACAAGGGAAACT+GGG + chr7_4:52388969-52388988 None:intergenic 45.0%
ACATGCTCTACAAGGGAAAC+TGG + chr7_4:52388970-52388989 None:intergenic 45.0%
CCTCTCAACATGCTCTACAA+GGG + chr7_4:52388977-52388996 None:intergenic 45.0%
CCTTGTAGAGCATGTTGAGA+GGG - chr7_4:52388975-52388994 Msa1162010:five_prime_UTR 45.0%
!! AAAAAAAAAAAAAAAAATAG+TGG + chr7_4:52386932-52386951 None:intergenic 5.0%
AGACGATTCCCTCCCATTTC+TGG - chr7_4:52386846-52386865 Msa1162010:three_prime_UTR 50.0%
! CCCTCCCATTTCTGGATTTC+TGG - chr7_4:52386854-52386873 Msa1162010:three_prime_UTR 50.0%
ACTCCCGAATTCCCAGATCT+AGG - chr7_4:52387039-52387058 Msa1162010:CDS 50.0%
GAGCAATGCTTGCCTAGATC+TGG + chr7_4:52387054-52387073 None:intergenic 50.0%
AGGCCAGTAAGGTTGTTGCA+TGG - chr7_4:52387909-52387928 Msa1162010:intron 50.0%
CTTCTCCAGTTCTTGGGGTT+TGG - chr7_4:52388198-52388217 Msa1162010:intron 50.0%
CGAACCCCTTTAGCACAAAG+TGG + chr7_4:52388394-52388413 None:intergenic 50.0%
!! AAGAAAAAGTGTCCTGCCCC+AGG - chr7_4:52388502-52388521 Msa1162010:intron 50.0%
TAAGGTCTCTCTGCATCCTG+GGG + chr7_4:52388521-52388540 None:intergenic 50.0%
CTAAGGTCTCTCTGCATCCT+GGG + chr7_4:52388522-52388541 None:intergenic 50.0%
ACTAAGGTCTCTCTGCATCC+TGG + chr7_4:52388523-52388542 None:intergenic 50.0%
GGACCTGATCACAAATGTCC+TGG - chr7_4:52388604-52388623 Msa1162010:intron 50.0%
GGTCCAGGACATTTGTGATC+AGG + chr7_4:52388610-52388629 None:intergenic 50.0%
CCCTTGTAGAGCATGTTGAG+AGG - chr7_4:52388974-52388993 Msa1162010:five_prime_UTR 50.0%
CCCTCTCAACATGCTCTACA+AGG + chr7_4:52388978-52388997 None:intergenic 50.0%
GAGCATGTTGAGAGGGATCA+TGG - chr7_4:52388982-52389001 Msa1162010:five_prime_UTR 50.0%
CATGTTGAGAGGGATCATGG+TGG - chr7_4:52388985-52389004 Msa1162010:five_prime_UTR 50.0%
AGTGGGATAGGACAGAGCAG+TGG - chr7_4:52388766-52388785 Msa1162010:intron 55.0%
ATAGGACAGAGCAGTGGCAG+TGG - chr7_4:52388772-52388791 Msa1162010:intron 55.0%
! GTTTTCCTCGGCCACGAGTT+TGG - chr7_4:52388840-52388859 Msa1162010:CDS 55.0%
CGCATGATCCACCAAACTCG+TGG + chr7_4:52388854-52388873 None:intergenic 55.0%
AGGAGTGGCGGCAACCAATA+TGG - chr7_4:52388889-52388908 Msa1162010:CDS 55.0%
GGAGTGGCGGCAACCAATAT+GGG - chr7_4:52388890-52388909 Msa1162010:CDS 55.0%
GCATGCCCCAAGTGCAGTAA+AGG - chr7_4:52388934-52388953 Msa1162010:five_prime_UTR 55.0%
TTCCTCGGCCACGAGTTTGG+TGG - chr7_4:52388843-52388862 Msa1162010:CDS 60.0%
ATCCACCAAACTCGTGGCCG+AGG + chr7_4:52388848-52388867 None:intergenic 60.0%
GTCTCTCTGCATCCTGGGGC+AGG + chr7_4:52388517-52388536 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 52386690 52389256 52386690 ID=Msa1162010;Name=Msa1162010
chr7_4 mRNA 52386690 52389256 52386690 ID=Msa1162010-mRNA-1;Parent=Msa1162010;Name=Msa1162010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=343|1|1|1|1|1|2|239|268
chr7_4 exon 52386690 52387636 52386690 ID=Msa1162010-mRNA-1:exon:15061;Parent=Msa1162010-mRNA-1
chr7_4 exon 52388815 52389256 52388815 ID=Msa1162010-mRNA-1:exon:15060;Parent=Msa1162010-mRNA-1
chr7_4 five_prime_UTR 52388914 52389256 52388914 ID=Msa1162010-mRNA-1:five_prime_utr;Parent=Msa1162010-mRNA-1
chr7_4 CDS 52388815 52388913 52388815 ID=Msa1162010-mRNA-1:cds;Parent=Msa1162010-mRNA-1
chr7_4 CDS 52386929 52387636 52386929 ID=Msa1162010-mRNA-1:cds;Parent=Msa1162010-mRNA-1
chr7_4 three_prime_UTR 52386690 52386928 52386690 ID=Msa1162010-mRNA-1:three_prime_utr;Parent=Msa1162010-mRNA-1
Gene Sequence

>Msa1162010

ATGGGGACTCCCGAATTCCCAGATCTAGGCAAGCATTGCTCTGTTTCTGATTGCAGACTCATCGATTTCTTGCCCTTCACTTGTGATCGTTGCAATCAGGTATATTGTTTGGGGCACCGAAGTTATATTGAGCATAATTGTACAAAACCCAACAAACAAGATGTCACCGTTGTTATATGTCCACTTTGTGCTAAAGGGGTTCGCCTAATTCCAGACCAAGATCCAAACATAGTTTGGGAGCGTCATGTGAACACAGACTGTGATCCATCAAATTATGAAAAAGCCACAAAGAAGAAAAAGTGTCCTGCCCCAGGATGCAGAGAGACCTTAGTATTCTCAAACACAATCAAGTGTAGGGACTGTGAGGTTGACCATTGTTTGAAGCACAGGTTTGGACCTGATCACAAATGTCCTGGACCCAAAAAGTTGGAAACAAGTTTTTCATTTATGAGTCTTTGGAATATGAGTAATGGTAAAGAAGAGTCAAAACCCAATTTAACTTCAACCACGTCATCTAAGTGGACTTCAAGCCTTCTTGACTTGGCTTCTAAATTGAGTGGGATAGGACAGAGCAGTGGCAGTGGTACTAGTAATAATCAACTGGAGAAGTGTCCACTATGCGATGCTAAGTTTTCCTCGGCCACGAGTTTGGTGGATCATGCGAAAAAAGTTCATCAAAGGAGTGGCGGCAACCAATATGGGACAAAGAAGGTGTCGATTTCTGCATGCCCCAAGTGCAGTAAAGGTTTTCTGGACCCAGTTTCCCTTGTAGAGCATGTTGAGAGGGATCATGGTGGTTGTTCTTGA
Protein sequence

>Msa1162010

MGTPEFPDLGKHCSVSDCRLIDFLPFTCDRCNQVYCLGHRSYIEHNCTKPNKQDVTVVICPLCAKGVRLIPDQDPNIVWERHVNTDCDPSNYEKATKKKKCPAPGCRETLVFSNTIKCRDCEVDHCLKHRFGPDHKCPGPKKLETSFSFMSLWNMSNGKEESKPNLTSTTSSKWTSSLLDLASKLSGIGQSSGSGTSNNQLEKCPLCDAKFSSATSLVDHAKKVHQRSGGNQYGTKKVSISACPKCSKGFLDPVSLVEHVERDHGGCS*