AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1114960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114960 MtrunA17_Chr7g0257681 97.388 268 7 0 1 268 1 268 0.0 540
Msa1114960 MtrunA17_Chr1g0173421 67.596 287 72 4 1 268 1 285 2.02e-133 378
Msa1114960 MtrunA17_Chr6g0451591 42.675 157 82 3 2 154 50 202 3.12e-36 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114960 AT2G41835.1 58.803 284 94 5 1 268 1 277 4.56e-112 324
Msa1114960 AT3G57480.2 58.209 268 91 4 1 268 1 247 1.45e-103 301
Msa1114960 AT3G57480.1 58.209 268 91 4 1 268 1 247 1.45e-103 301
Msa1114960 AT3G28210.2 44.872 156 77 4 2 154 5 154 7.15e-36 127
Msa1114960 AT3G28210.1 44.872 156 77 4 2 154 5 154 7.15e-36 127
Msa1114960 AT5G48205.1 55.046 109 21 3 1 106 1 84 9.01e-29 108

Find 104 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCCCATTTCTGGATTTC+TGG -0.035419 7_3:-61974988 Msa1114960:five_prime_UTR
AGACGATTCCCTCCCATTTC+TGG 0.113556 7_3:-61974996 Msa1114960:five_prime_UTR
GTTCATTAAATAATTTATTT+TGG 0.197602 7_3:+61975068 None:intergenic
TGACGTGGTTGAAGTTAAAT+TGG 0.210618 7_3:+61973131 None:intergenic
AATTATTAGAGCGTGGAATT+AGG 0.225673 7_3:-61975023 Msa1114960:five_prime_UTR
TTGGATCTTGGTCTGGAATT+AGG 0.236801 7_3:+61973418 None:intergenic
TAATTAGAGAATCATAATTT+TGG 0.240465 7_3:+61975046 None:intergenic
AGGAGTGGCGGCAACCAATA+TGG 0.258450 7_3:-61972943 Msa1114960:CDS
AATGAATAGCACTTTCTTGA+TGG 0.275542 7_3:-61972791 Msa1114960:three_prime_UTR
ACCAAGATCCAAACATAGTT+TGG 0.297216 7_3:-61973407 Msa1114960:CDS
TTCTGGATTTCTGGTTGGTT+GGG 0.311039 7_3:-61974979 Msa1114960:five_prime_UTR
CTTGCCTAGATCTGGGAATT+CGG 0.320931 7_3:+61974805 None:intergenic
ACATGCTCTACAAGGGAAAC+TGG 0.328561 7_3:+61972865 None:intergenic
TTTGAGCTTGGTTTGGATCA+TGG 0.341751 7_3:-61974834 Msa1114960:exon
ATTCTACTACTCGTTGTCTT+TGG 0.343219 7_3:-61975102 Msa1114960:five_prime_UTR
ATTATTAGAGCGTGGAATTA+GGG 0.343452 7_3:-61975022 Msa1114960:five_prime_UTR
CCCAAACTATGTTTGGATCT+TGG 0.346442 7_3:+61973406 None:intergenic
TCTTCGGTTTGAGCTTGGTT+TGG 0.348637 7_3:-61974841 Msa1114960:five_prime_UTR
GACTTCAAGCCTTCTTGACT+TGG 0.353989 7_3:-61973100 Msa1114960:CDS
CACTTGTGATCGTTGCAATC+AGG 0.365381 7_3:-61974738 Msa1114960:intron
CCCATTTCTGGATTTCTGGT+TGG 0.366874 7_3:-61974984 Msa1114960:five_prime_UTR
TTCATACATACACCGATATA+CGG 0.368726 7_3:-61972630 Msa1114960:three_prime_UTR
GAGCAATGCTTGCCTAGATC+TGG 0.369502 7_3:+61974797 None:intergenic
AACGGTGACATCTTGTTTGT+TGG 0.370225 7_3:+61973473 None:intergenic
AGGATTTCGTTTCTTCTCTT+CGG 0.372070 7_3:-61974857 Msa1114960:five_prime_UTR
TATGTCCACTTTGTGCTAAA+GGG 0.378295 7_3:-61973446 Msa1114960:CDS
TTTCTGGATTTCTGGTTGGT+TGG 0.383815 7_3:-61974980 Msa1114960:five_prime_UTR
CAGAAAACCTTTACTGCACT+TGG 0.385251 7_3:+61972891 None:intergenic
GGTCCAGGACATTTGTGATC+AGG 0.386396 7_3:+61973225 None:intergenic
ACACCTTCTTTGCCCCATAT+TGG 0.386967 7_3:+61972929 None:intergenic
TTGTGAATTACGCGGATATA+GGG 0.387535 7_3:+61974940 None:intergenic
TTGAGCTTGGTTTGGATCAT+GGG 0.395085 7_3:-61974833 Msa1114960:exon
GGAGTGGCGGCAACCAATAT+GGG 0.400426 7_3:-61972942 Msa1114960:CDS
ACCAACCAGAAATCCAGAAA+TGG 0.401843 7_3:+61974983 None:intergenic
TGACGCTCCCAAACTATGTT+TGG 0.404477 7_3:+61973399 None:intergenic
CATTGTTTGAAGCACAGGTT+TGG 0.404986 7_3:-61973249 Msa1114960:CDS
ACTAAGGTCTCTCTGCATCC+TGG 0.410212 7_3:+61973312 None:intergenic
ATATGTCCACTTTGTGCTAA+AGG 0.412254 7_3:-61973447 Msa1114960:CDS
TTGACCATTGTTTGAAGCAC+AGG 0.415680 7_3:-61973254 Msa1114960:CDS
TTGCCTAGATCTGGGAATTC+GGG 0.416402 7_3:+61974806 None:intergenic
TACTCGTTGTCTTTGGCAAA+TGG 0.417251 7_3:-61975095 Msa1114960:five_prime_UTR
ATATTAGATTTATTTGATCA+TGG 0.419688 7_3:-61972696 Msa1114960:three_prime_UTR
AGAAGAATCAATCCGTATAT+CGG 0.427532 7_3:+61972618 None:intergenic
TCGGTGTATGTATGAATTGT+AGG 0.428418 7_3:+61972637 None:intergenic
CTAAGGTCTCTCTGCATCCT+GGG 0.434135 7_3:+61973313 None:intergenic
GACGTGGTTGAAGTTAAATT+GGG 0.435317 7_3:+61973132 None:intergenic
ACTATGTTTGGATCTTGGTC+TGG 0.435501 7_3:+61973411 None:intergenic
ACGGTGACATCTTGTTTGTT+GGG 0.438220 7_3:+61973474 None:intergenic
TCTTCTCTTCGGTTTGAGCT+TGG 0.443261 7_3:-61974846 Msa1114960:five_prime_UTR
CCCTCTCAACATGCTCTACA+AGG 0.443451 7_3:+61972857 None:intergenic
ATTGTGAATTACGCGGATAT+AGG 0.451113 7_3:+61974939 None:intergenic
CCAAGATCCAAACATAGTTT+GGG 0.458482 7_3:-61973406 Msa1114960:CDS
GTCTCTCTGCATCCTGGGGC+AGG 0.461132 7_3:+61973318 None:intergenic
CTTCTTTGTTGTAATGCTGT+TGG 0.468468 7_3:-61972600 Msa1114960:three_prime_UTR
TGGTACTAGTAATAATCAAC+TGG 0.476635 7_3:-61973040 Msa1114960:CDS
GATTGTGTTTGAGAATACTA+AGG 0.477004 7_3:+61973296 None:intergenic
GCATGCCCCAAGTGCAGTAA+AGG 0.479086 7_3:-61972898 Msa1114960:CDS
TATTAGATTTATTTGATCAT+GGG 0.481987 7_3:-61972695 Msa1114960:three_prime_UTR
GCTTCTAAATTGAGTGGGAT+AGG 0.483203 7_3:-61973078 Msa1114960:CDS
CCAACCAGAAATCCAGAAAT+GGG 0.503012 7_3:+61974984 None:intergenic
ATCCACCAAACTCGTGGCCG+AGG 0.511045 7_3:+61972987 None:intergenic
CAACCAATATGGGGCAAAGA+AGG 0.517022 7_3:-61972932 Msa1114960:CDS
TTCCTCGGCCACGAGTTTGG+TGG 0.518637 7_3:-61972989 Msa1114960:CDS
CTCAAACACAATCAAGTGTA+GGG 0.519840 7_3:-61973286 Msa1114960:CDS
ATTTAGAAGCCAAGTCAAGA+AGG 0.521688 7_3:+61973091 None:intergenic
GGACCTGATCACAAATGTCC+TGG 0.523810 7_3:-61973228 Msa1114960:CDS
AGAAAACCTTTACTGCACTT+GGG 0.532761 7_3:+61972892 None:intergenic
CATGCTCTACAAGGGAAACT+GGG 0.533711 7_3:+61972866 None:intergenic
ACTTGGCTTCTAAATTGAGT+GGG 0.535363 7_3:-61973083 Msa1114960:CDS
TGAGCTTGGTTTGGATCATG+GGG 0.539843 7_3:-61974832 Msa1114960:exon
CTTCAACCACGTCATCTAAG+TGG 0.540578 7_3:-61973122 Msa1114960:CDS
CCTTGTAGAGCATGTTGAGA+GGG 0.543468 7_3:-61972857 Msa1114960:CDS
ACTCCCGAATTCCCAGATCT+AGG 0.548420 7_3:-61974809 Msa1114960:CDS
GTTGTTCTTGAGAATTGTTG+TGG 0.549442 7_3:-61972825 Msa1114960:exon
GACTTGGCTTCTAAATTGAG+TGG 0.550800 7_3:-61973084 Msa1114960:CDS
AGCAATGCTTGCCTAGATCT+GGG 0.554462 7_3:+61974798 None:intergenic
CAAACCTGTGCTTCAAACAA+TGG 0.566927 7_3:+61973250 None:intergenic
GAGCATGTTGAGAGGGATCA+TGG 0.569049 7_3:-61972850 Msa1114960:CDS
ATGTCCACTTTGTGCTAAAG+GGG 0.570588 7_3:-61973445 Msa1114960:CDS
GAAAACCTTTACTGCACTTG+GGG 0.571579 7_3:+61972893 None:intergenic
TCTCAAACACAATCAAGTGT+AGG 0.571728 7_3:-61973287 Msa1114960:CDS
ATTAGAGCGTGGAATTAGGG+AGG 0.572050 7_3:-61975019 Msa1114960:five_prime_UTR
CTAATTAAATTATTAGAGCG+TGG 0.572217 7_3:-61975030 Msa1114960:five_prime_UTR
ATAGGACAGAGCAGTGGCAG+TGG 0.577401 7_3:-61973060 Msa1114960:CDS
CGCATGATCCACCAAACTCG+TGG 0.584791 7_3:+61972981 None:intergenic
ATTGCAACGATCACAAGTGA+AGG 0.586850 7_3:+61974742 None:intergenic
AGTCTTTGGAATATGAGTAA+TGG 0.594698 7_3:-61973171 Msa1114960:CDS
AATTATGCTCAATATAACTT+CGG 0.599455 7_3:+61973505 None:intergenic
CCCTTGTAGAGCATGTTGAG+AGG 0.611040 7_3:-61972858 Msa1114960:CDS
CCAGAAATCCAGAAATGGGA+GGG 0.613406 7_3:+61974988 None:intergenic
GAAAACTTAGCATCACATAG+TGG 0.613925 7_3:+61973009 None:intergenic
CGAACCCCTTTAGCACAAAG+TGG 0.621156 7_3:+61973441 None:intergenic
AAAGTTCATCAAAGGAGTGG+CGG 0.638449 7_3:-61972955 Msa1114960:CDS
ACCAGAAATCCAGAAATGGG+AGG 0.639433 7_3:+61974987 None:intergenic
TGAAGTCCACTTAGATGACG+TGG 0.643218 7_3:+61973116 None:intergenic
TTGCAACGATCACAAGTGAA+GGG 0.653221 7_3:+61974743 None:intergenic
CCTCTCAACATGCTCTACAA+GGG 0.654661 7_3:+61972858 None:intergenic
TAAGGTCTCTCTGCATCCTG+GGG 0.659577 7_3:+61973314 None:intergenic
GAGTGGCGGCAACCAATATG+GGG 0.665147 7_3:-61972941 Msa1114960:CDS
AATCAAGTGTAGGGACTGTG+AGG 0.678787 7_3:-61973277 Msa1114960:CDS
AGTGGGATAGGACAGAGCAG+TGG 0.703021 7_3:-61973066 Msa1114960:CDS
GTGGAGAATTGTGAATTACG+CGG 0.729104 7_3:+61974932 None:intergenic
ACAAAGTGGACATATAACAA+CGG 0.739755 7_3:+61973455 None:intergenic
CATGTTGAGAGGGATCATGG+TGG 0.808965 7_3:-61972847 Msa1114960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+TGG - chr7_3:61973564-61973583 Msa1114960:intron 0.0%
!!! GTTCATTAAATAATTTATTT+TGG + chr7_3:61972685-61972704 None:intergenic 10.0%
!! CAAAAAAAAAAAAAAAATAG+TGG + chr7_3:61972840-61972859 None:intergenic 10.0%
!! ATATATATATATATTGGTAT+TGG - chr7_3:61973570-61973589 Msa1114960:intron 10.0%
!!! GTTATTTTCATAAATTATTT+TGG - chr7_3:61973986-61974005 Msa1114960:intron 10.0%
!!! TAATTAGAGAATCATAATTT+TGG + chr7_3:61972707-61972726 None:intergenic 15.0%
!! TTTGTTTCGAAATTAATTAA+TGG - chr7_3:61973059-61973078 Msa1114960:CDS 15.0%
!! TTGTTTCGAAATTAATTAAT+GGG - chr7_3:61973060-61973079 Msa1114960:CDS 15.0%
!!! TATCAGTTTATTTTAGAATA+AGG - chr7_3:61973595-61973614 Msa1114960:intron 15.0%
!! ATATTAGATTTATTTGATCA+TGG - chr7_3:61975054-61975073 Msa1114960:five_prime_UTR 15.0%
!! TATTAGATTTATTTGATCAT+GGG - chr7_3:61975055-61975074 Msa1114960:five_prime_UTR 15.0%
!! GAAAAAAAATAAATGAGGAA+TGG + chr7_3:61972869-61972888 None:intergenic 20.0%
!!! TTCCTCATTTATTTTTTTTC+TGG - chr7_3:61972869-61972888 Msa1114960:CDS 20.0%
!! TTCCAGAAAAAAAATAAATG+AGG + chr7_3:61972874-61972893 None:intergenic 20.0%
!!! TCATTTATTTTTTTTCTGGA+AGG - chr7_3:61972873-61972892 Msa1114960:CDS 20.0%
!! AGAAAATAATAGAATGAAAC+GGG + chr7_3:61973110-61973129 None:intergenic 20.0%
!! GAGAAAATAATAGAATGAAA+CGG + chr7_3:61973111-61973130 None:intergenic 20.0%
!!! TTTCATTCTATTATTTTCTC+CGG - chr7_3:61973111-61973130 Msa1114960:CDS 20.0%
!!! GAAACAATGTTTTTAATCAA+AGG + chr7_3:61973170-61973189 None:intergenic 20.0%
!!! AGGATTTTTTCAAAATGAAA+TGG - chr7_3:61973615-61973634 Msa1114960:intron 20.0%
!! TGATTATTTCATTTGAGAAA+TGG - chr7_3:61973683-61973702 Msa1114960:intron 20.0%
!! TGAATTTATTGAAATAAGCT+AGG - chr7_3:61974009-61974028 Msa1114960:intron 20.0%
!! AATCACTTACATAAAATGTT+TGG + chr7_3:61974054-61974073 None:intergenic 20.0%
!! AATTATGCTCAATATAACTT+CGG + chr7_3:61974248-61974267 None:intergenic 20.0%
!!! CAATATTTTGCTATCAAATA+GGG + chr7_3:61975012-61975031 None:intergenic 20.0%
!!! ACAATATTTTGCTATCAAAT+AGG + chr7_3:61975013-61975032 None:intergenic 20.0%
! CTAATTAAATTATTAGAGCG+TGG - chr7_3:61972720-61972739 Msa1114960:three_prime_UTR 25.0%
!! TTCATTCTATTATTTTCTCC+GGG - chr7_3:61973112-61973131 Msa1114960:CDS 25.0%
!! TATTGATTATAGCAATAACC+CGG + chr7_3:61973133-61973152 None:intergenic 25.0%
! CACAATTTAATCCAACAATA+TGG + chr7_3:61973264-61973283 None:intergenic 25.0%
! TATTGTTGGATTAAATTGTG+TGG - chr7_3:61973264-61973283 Msa1114960:CDS 25.0%
! GTTTCACAAAACTATAGTAA+TGG + chr7_3:61973647-61973666 None:intergenic 25.0%
! ACATAAAGTAAATCATTTGC+TGG - chr7_3:61973725-61973744 Msa1114960:intron 25.0%
!! TATCATCTATATAGAGTGTT+TGG - chr7_3:61973853-61973872 Msa1114960:intron 25.0%
! GTAACTTAATTAAGTGCTTA+CGG - chr7_3:61973880-61973899 Msa1114960:intron 25.0%
! ATTTATTGAAATAAGCTAGG+AGG - chr7_3:61974012-61974031 Msa1114960:intron 25.0%
!!! AAACTTGTTTCCAACTTTTT+GGG + chr7_3:61974549-61974568 None:intergenic 25.0%
!!! AAAACTTGTTTCCAACTTTT+TGG + chr7_3:61974550-61974569 None:intergenic 25.0%
!! GTTTTTCATTTATGAGTCTT+TGG - chr7_3:61974565-61974584 Msa1114960:intron 25.0%
AATTATTAGAGCGTGGAATT+AGG - chr7_3:61972727-61972746 Msa1114960:three_prime_UTR 30.0%
ATTATTAGAGCGTGGAATTA+GGG - chr7_3:61972728-61972747 Msa1114960:three_prime_UTR 30.0%
! ATTATCGGTTGAACTAACTA+AGG + chr7_3:61973239-61973258 None:intergenic 30.0%
TCCAACAATATGGACATTAT+CGG + chr7_3:61973254-61973273 None:intergenic 30.0%
! CGTTTAAGTGTTTGACTTAT+AGG - chr7_3:61973348-61973367 Msa1114960:CDS 30.0%
! TTAAGTGTTTGACTTATAGG+AGG - chr7_3:61973351-61973370 Msa1114960:CDS 30.0%
!!! TGTTAGTTTTTCCTGTTGTT+AGG + chr7_3:61973446-61973465 None:intergenic 30.0%
! ATAGAATTGACTTTGAAAGC+TGG - chr7_3:61973536-61973555 Msa1114960:intron 30.0%
CTTCACTGAAGTTTCTATTA+AGG - chr7_3:61973789-61973808 Msa1114960:intron 30.0%
AGTTGCTTAGTTGAAAACAA+GGG + chr7_3:61974169-61974188 None:intergenic 30.0%
AACAATATACCTGCAAAAGA+TGG + chr7_3:61974218-61974237 None:intergenic 30.0%
! TCTTTTGCAGGTATATTGTT+TGG - chr7_3:61974218-61974237 Msa1114960:intron 30.0%
! CTTTTGCAGGTATATTGTTT+GGG - chr7_3:61974219-61974238 Msa1114960:intron 30.0%
! TTTTGCAGGTATATTGTTTG+GGG - chr7_3:61974220-61974239 Msa1114960:intron 30.0%
ACAAAGTGGACATATAACAA+CGG + chr7_3:61974298-61974317 None:intergenic 30.0%
!!! GTGGCTTTTTCATAATTTGA+TGG + chr7_3:61974396-61974415 None:intergenic 30.0%
GATTGTGTTTGAGAATACTA+AGG + chr7_3:61974457-61974476 None:intergenic 30.0%
!! AGTCTTTGGAATATGAGTAA+TGG - chr7_3:61974579-61974598 Msa1114960:intron 30.0%
TGGTACTAGTAATAATCAAC+TGG - chr7_3:61974710-61974729 Msa1114960:intron 30.0%
ATGCGAAAAAAGTTCATCAA+AGG - chr7_3:61974787-61974806 Msa1114960:CDS 30.0%
AAAAAAGTTCATCAAAGGAG+TGG - chr7_3:61974792-61974811 Msa1114960:CDS 30.0%
AATGAATAGCACTTTCTTGA+TGG - chr7_3:61974959-61974978 Msa1114960:five_prime_UTR 30.0%
GCTCGCAAATAACAAAAAAT+AGG + chr7_3:61975094-61975113 None:intergenic 30.0%
TTCATACATACACCGATATA+CGG - chr7_3:61975120-61975139 Msa1114960:five_prime_UTR 30.0%
AGAAGAATCAATCCGTATAT+CGG + chr7_3:61975135-61975154 None:intergenic 30.0%
ATTCTACTACTCGTTGTCTT+TGG - chr7_3:61972648-61972667 Msa1114960:three_prime_UTR 35.0%
TTGTGAATTACGCGGATATA+GGG + chr7_3:61972813-61972832 None:intergenic 35.0%
ATTGTGAATTACGCGGATAT+AGG + chr7_3:61972814-61972833 None:intergenic 35.0%
AGGATTTCGTTTCTTCTCTT+CGG - chr7_3:61972893-61972912 Msa1114960:CDS 35.0%
ACCGATAATGTCCATATTGT+TGG - chr7_3:61973250-61973269 Msa1114960:CDS 35.0%
! TGTTTGACTTATAGGAGGTT+GGG - chr7_3:61973356-61973375 Msa1114960:CDS 35.0%
TTGCTGGAAATAATGAACTG+AGG - chr7_3:61973741-61973760 Msa1114960:intron 35.0%
AAGGTGAATGCTTAATGCAA+AGG - chr7_3:61973808-61973827 Msa1114960:intron 35.0%
!!! ATTTTGACTTCTCCAGTTCT+TGG - chr7_3:61974110-61974129 Msa1114960:intron 35.0%
!!! TTTTGACTTCTCCAGTTCTT+GGG - chr7_3:61974111-61974130 Msa1114960:intron 35.0%
TTGGAAATATGTAGCTGTGA+TGG - chr7_3:61974135-61974154 Msa1114960:intron 35.0%
GAGTTGCTTAGTTGAAAACA+AGG + chr7_3:61974170-61974189 None:intergenic 35.0%
AACTAAGCAACTCTTTGTGT+AGG - chr7_3:61974177-61974196 Msa1114960:intron 35.0%
ATATGTCCACTTTGTGCTAA+AGG - chr7_3:61974303-61974322 Msa1114960:intron 35.0%
TATGTCCACTTTGTGCTAAA+GGG - chr7_3:61974304-61974323 Msa1114960:intron 35.0%
ACCAAGATCCAAACATAGTT+TGG - chr7_3:61974343-61974362 Msa1114960:intron 35.0%
CCAAGATCCAAACATAGTTT+GGG - chr7_3:61974344-61974363 Msa1114960:intron 35.0%
!!! AGGACACTTTTTCTTCTTTG+TGG + chr7_3:61974415-61974434 None:intergenic 35.0%
TCTCAAACACAATCAAGTGT+AGG - chr7_3:61974463-61974482 Msa1114960:intron 35.0%
CTCAAACACAATCAAGTGTA+GGG - chr7_3:61974464-61974483 Msa1114960:intron 35.0%
GACGTGGTTGAAGTTAAATT+GGG + chr7_3:61974621-61974640 None:intergenic 35.0%
TGACGTGGTTGAAGTTAAAT+TGG + chr7_3:61974622-61974641 None:intergenic 35.0%
ATTTAGAAGCCAAGTCAAGA+AGG + chr7_3:61974662-61974681 None:intergenic 35.0%
ACTTGGCTTCTAAATTGAGT+GGG - chr7_3:61974667-61974686 Msa1114960:intron 35.0%
GAAAACTTAGCATCACATAG+TGG + chr7_3:61974744-61974763 None:intergenic 35.0%
! ATGTGATGCTAAGTTTTCCT+CGG - chr7_3:61974746-61974765 Msa1114960:CDS 35.0%
AGAAAACCTTTACTGCACTT+GGG + chr7_3:61974861-61974880 None:intergenic 35.0%
GTTGTTCTTGAGAATTGTTG+TGG - chr7_3:61974925-61974944 Msa1114960:five_prime_UTR 35.0%
TCGGTGTATGTATGAATTGT+AGG + chr7_3:61975116-61975135 None:intergenic 35.0%
! CTTCTTTGTTGTAATGCTGT+TGG - chr7_3:61975150-61975169 Msa1114960:five_prime_UTR 35.0%
TACTCGTTGTCTTTGGCAAA+TGG - chr7_3:61972655-61972674 Msa1114960:three_prime_UTR 40.0%
CCAACCAGAAATCCAGAAAT+GGG + chr7_3:61972769-61972788 None:intergenic 40.0%
ACCAACCAGAAATCCAGAAA+TGG + chr7_3:61972770-61972789 None:intergenic 40.0%
!! TTTCTGGATTTCTGGTTGGT+TGG - chr7_3:61972770-61972789 Msa1114960:three_prime_UTR 40.0%
!! TTCTGGATTTCTGGTTGGTT+GGG - chr7_3:61972771-61972790 Msa1114960:three_prime_UTR 40.0%
GTGGAGAATTGTGAATTACG+CGG + chr7_3:61972821-61972840 None:intergenic 40.0%
!! TTTGAGCTTGGTTTGGATCA+TGG - chr7_3:61972916-61972935 Msa1114960:CDS 40.0%
!! TTGAGCTTGGTTTGGATCAT+GGG - chr7_3:61972917-61972936 Msa1114960:CDS 40.0%
TTGCAACGATCACAAGTGAA+GGG + chr7_3:61973010-61973029 None:intergenic 40.0%
ATTGCAACGATCACAAGTGA+AGG + chr7_3:61973011-61973030 None:intergenic 40.0%
! GTGTTTGACTTATAGGAGGT+TGG - chr7_3:61973355-61973374 Msa1114960:CDS 40.0%
AAGGGTTAAGAAGTTGGAGT+TGG - chr7_3:61973403-61973422 Msa1114960:CDS 40.0%
AGAGTTCAAGTCCTAACAAC+AGG - chr7_3:61973432-61973451 Msa1114960:CDS 40.0%
AAACCATGCAACAACCTTAC+TGG + chr7_3:61973834-61973853 None:intergenic 40.0%
! ATGTTTGGCTCATGTCCATA+GGG + chr7_3:61974039-61974058 None:intergenic 40.0%
! AATGTTTGGCTCATGTCCAT+AGG + chr7_3:61974040-61974059 None:intergenic 40.0%
! TTTGACTTCTCCAGTTCTTG+GGG - chr7_3:61974112-61974131 Msa1114960:intron 40.0%
ATATTTCCAACCCCAAGAAC+TGG + chr7_3:61974125-61974144 None:intergenic 40.0%
! ATATCAGCTCCATCTTTTGC+AGG - chr7_3:61974206-61974225 Msa1114960:intron 40.0%
! ACGGTGACATCTTGTTTGTT+GGG + chr7_3:61974279-61974298 None:intergenic 40.0%
! AACGGTGACATCTTGTTTGT+TGG + chr7_3:61974280-61974299 None:intergenic 40.0%
ATGTCCACTTTGTGCTAAAG+GGG - chr7_3:61974305-61974324 Msa1114960:intron 40.0%
! TTGGATCTTGGTCTGGAATT+AGG + chr7_3:61974335-61974354 None:intergenic 40.0%
ACTATGTTTGGATCTTGGTC+TGG + chr7_3:61974342-61974361 None:intergenic 40.0%
CCCAAACTATGTTTGGATCT+TGG + chr7_3:61974347-61974366 None:intergenic 40.0%
! TTGACCATTGTTTGAAGCAC+AGG - chr7_3:61974496-61974515 Msa1114960:intron 40.0%
CAAACCTGTGCTTCAAACAA+TGG + chr7_3:61974503-61974522 None:intergenic 40.0%
! CATTGTTTGAAGCACAGGTT+TGG - chr7_3:61974501-61974520 Msa1114960:intron 40.0%
GACTTGGCTTCTAAATTGAG+TGG - chr7_3:61974666-61974685 Msa1114960:intron 40.0%
GCTTCTAAATTGAGTGGGAT+AGG - chr7_3:61974672-61974691 Msa1114960:intron 40.0%
AAAGTTCATCAAAGGAGTGG+CGG - chr7_3:61974795-61974814 Msa1114960:CDS 40.0%
GAAAACCTTTACTGCACTTG+GGG + chr7_3:61974860-61974879 None:intergenic 40.0%
CAGAAAACCTTTACTGCACT+TGG + chr7_3:61974862-61974881 None:intergenic 40.0%
! CAAGTGCAGTAAAGGTTTTC+TGG - chr7_3:61974860-61974879 Msa1114960:five_prime_UTR 40.0%
ATTAGAGCGTGGAATTAGGG+AGG - chr7_3:61972731-61972750 Msa1114960:three_prime_UTR 45.0%
CCAGAAATCCAGAAATGGGA+GGG + chr7_3:61972765-61972784 None:intergenic 45.0%
ACCAGAAATCCAGAAATGGG+AGG + chr7_3:61972766-61972785 None:intergenic 45.0%
!! CCCATTTCTGGATTTCTGGT+TGG - chr7_3:61972766-61972785 Msa1114960:three_prime_UTR 45.0%
! TCTTCTCTTCGGTTTGAGCT+TGG - chr7_3:61972904-61972923 Msa1114960:CDS 45.0%
! TCTTCGGTTTGAGCTTGGTT+TGG - chr7_3:61972909-61972928 Msa1114960:CDS 45.0%
!! TGAGCTTGGTTTGGATCATG+GGG - chr7_3:61972918-61972937 Msa1114960:CDS 45.0%
TTGCCTAGATCTGGGAATTC+GGG + chr7_3:61972947-61972966 None:intergenic 45.0%
CTTGCCTAGATCTGGGAATT+CGG + chr7_3:61972948-61972967 None:intergenic 45.0%
AGCAATGCTTGCCTAGATCT+GGG + chr7_3:61972955-61972974 None:intergenic 45.0%
CACTTGTGATCGTTGCAATC+AGG - chr7_3:61973012-61973031 Msa1114960:CDS 45.0%
AACTAGTTCGAGCGAGCTAA+GGG - chr7_3:61973385-61973404 Msa1114960:CDS 45.0%
CGAGCTAAGGGTTAAGAAGT+TGG - chr7_3:61973397-61973416 Msa1114960:CDS 45.0%
GGTTAAGAAGTTGGAGTTGG+AGG - chr7_3:61973406-61973425 Msa1114960:CDS 45.0%
GCTTAATGCAAAGGCCAGTA+AGG - chr7_3:61973817-61973836 Msa1114960:intron 45.0%
AATAAGCTAGGAGGACCCTA+TGG - chr7_3:61974021-61974040 Msa1114960:intron 45.0%
TGACGCTCCCAAACTATGTT+TGG + chr7_3:61974354-61974373 None:intergenic 45.0%
AATCAAGTGTAGGGACTGTG+AGG - chr7_3:61974473-61974492 Msa1114960:intron 45.0%
ATGTCCTGGACCCAAAAAGT+TGG - chr7_3:61974536-61974555 Msa1114960:intron 45.0%
!!! GTTTCCAACTTTTTGGGTCC+AGG + chr7_3:61974543-61974562 None:intergenic 45.0%
CTTCAACCACGTCATCTAAG+TGG - chr7_3:61974628-61974647 Msa1114960:intron 45.0%
TGAAGTCCACTTAGATGACG+TGG + chr7_3:61974637-61974656 None:intergenic 45.0%
GACTTCAAGCCTTCTTGACT+TGG - chr7_3:61974650-61974669 Msa1114960:intron 45.0%
CAACCAATATGGGGCAAAGA+AGG - chr7_3:61974818-61974837 Msa1114960:CDS 45.0%
ACACCTTCTTTGCCCCATAT+TGG + chr7_3:61974824-61974843 None:intergenic 45.0%
CATGCTCTACAAGGGAAACT+GGG + chr7_3:61974887-61974906 None:intergenic 45.0%
ACATGCTCTACAAGGGAAAC+TGG + chr7_3:61974888-61974907 None:intergenic 45.0%
CCTCTCAACATGCTCTACAA+GGG + chr7_3:61974895-61974914 None:intergenic 45.0%
CCTTGTAGAGCATGTTGAGA+GGG - chr7_3:61974893-61974912 Msa1114960:five_prime_UTR 45.0%
AGACGATTCCCTCCCATTTC+TGG - chr7_3:61972754-61972773 Msa1114960:three_prime_UTR 50.0%
! CCCTCCCATTTCTGGATTTC+TGG - chr7_3:61972762-61972781 Msa1114960:three_prime_UTR 50.0%
ACTCCCGAATTCCCAGATCT+AGG - chr7_3:61972941-61972960 Msa1114960:CDS 50.0%
GAGCAATGCTTGCCTAGATC+TGG + chr7_3:61972956-61972975 None:intergenic 50.0%
CAACTAGTTCGAGCGAGCTA+AGG - chr7_3:61973384-61973403 Msa1114960:CDS 50.0%
AGGCCAGTAAGGTTGTTGCA+TGG - chr7_3:61973828-61973847 Msa1114960:intron 50.0%
ACTTCTCCAGTTCTTGGGGT+TGG - chr7_3:61974116-61974135 Msa1114960:intron 50.0%
CGAACCCCTTTAGCACAAAG+TGG + chr7_3:61974312-61974331 None:intergenic 50.0%
!! AAGAAAAAGTGTCCTGCCCC+AGG - chr7_3:61974420-61974439 Msa1114960:intron 50.0%
TAAGGTCTCTCTGCATCCTG+GGG + chr7_3:61974439-61974458 None:intergenic 50.0%
CTAAGGTCTCTCTGCATCCT+GGG + chr7_3:61974440-61974459 None:intergenic 50.0%
ACTAAGGTCTCTCTGCATCC+TGG + chr7_3:61974441-61974460 None:intergenic 50.0%
GGACCTGATCACAAATGTCC+TGG - chr7_3:61974522-61974541 Msa1114960:intron 50.0%
GGTCCAGGACATTTGTGATC+AGG + chr7_3:61974528-61974547 None:intergenic 50.0%
CCCTTGTAGAGCATGTTGAG+AGG - chr7_3:61974892-61974911 Msa1114960:five_prime_UTR 50.0%
CCCTCTCAACATGCTCTACA+AGG + chr7_3:61974896-61974915 None:intergenic 50.0%
GAGCATGTTGAGAGGGATCA+TGG - chr7_3:61974900-61974919 Msa1114960:five_prime_UTR 50.0%
CATGTTGAGAGGGATCATGG+TGG - chr7_3:61974903-61974922 Msa1114960:five_prime_UTR 50.0%
AGTGGGATAGGACAGAGCAG+TGG - chr7_3:61974684-61974703 Msa1114960:intron 55.0%
ATAGGACAGAGCAGTGGCAG+TGG - chr7_3:61974690-61974709 Msa1114960:intron 55.0%
! GTTTTCCTCGGCCACGAGTT+TGG - chr7_3:61974758-61974777 Msa1114960:CDS 55.0%
CGCATGATCCACCAAACTCG+TGG + chr7_3:61974772-61974791 None:intergenic 55.0%
AGGAGTGGCGGCAACCAATA+TGG - chr7_3:61974807-61974826 Msa1114960:CDS 55.0%
GGAGTGGCGGCAACCAATAT+GGG - chr7_3:61974808-61974827 Msa1114960:CDS 55.0%
GAGTGGCGGCAACCAATATG+GGG - chr7_3:61974809-61974828 Msa1114960:CDS 55.0%
GCATGCCCCAAGTGCAGTAA+AGG - chr7_3:61974852-61974871 Msa1114960:five_prime_UTR 55.0%
TTCCTCGGCCACGAGTTTGG+TGG - chr7_3:61974761-61974780 Msa1114960:CDS 60.0%
ATCCACCAAACTCGTGGCCG+AGG + chr7_3:61974766-61974785 None:intergenic 60.0%
GTCTCTCTGCATCCTGGGGC+AGG + chr7_3:61974435-61974454 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 61972598 61975174 61972598 ID=Msa1114960;Name=Msa1114960
chr7_3 mRNA 61972598 61975174 61972598 ID=Msa1114960-mRNA-1;Parent=Msa1114960;Name=Msa1114960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=337|1|1|1|1|1|2|239|268
chr7_3 exon 61972598 61973544 61972598 ID=Msa1114960-mRNA-1:exon:14237;Parent=Msa1114960-mRNA-1
chr7_3 exon 61974739 61975174 61974739 ID=Msa1114960-mRNA-1:exon:14236;Parent=Msa1114960-mRNA-1
chr7_3 five_prime_UTR 61974838 61975174 61974838 ID=Msa1114960-mRNA-1:five_prime_utr;Parent=Msa1114960-mRNA-1
chr7_3 CDS 61974739 61974837 61974739 ID=Msa1114960-mRNA-1:cds;Parent=Msa1114960-mRNA-1
chr7_3 CDS 61972837 61973544 61972837 ID=Msa1114960-mRNA-1:cds;Parent=Msa1114960-mRNA-1
chr7_3 three_prime_UTR 61972598 61972836 61972598 ID=Msa1114960-mRNA-1:three_prime_utr;Parent=Msa1114960-mRNA-1
Gene Sequence

>Msa1114960

ATGGGGACTCCCGAATTCCCAGATCTAGGCAAGCATTGCTCTGTTTCTGATTGCAGACTCATCGATTTCTTGCCCTTCACTTGTGATCGTTGCAATCAGGTATATTGTTTGGGGCACCGAAGTTATATTGAGCATAATTGTACAAAACCCAACAAACAAGATGTCACCGTTGTTATATGTCCACTTTGTGCTAAAGGGGTTCGCCTAATTCCAGACCAAGATCCAAACATAGTTTGGGAGCGTCATGTGAACACAGACTGTGATCCATCAAATTATGAAAAAGCCACAAAGAAGAAAAAGTGTCCTGCCCCAGGATGCAGAGAGACCTTAGTATTCTCAAACACAATCAAGTGTAGGGACTGTGAGGTTGACCATTGTTTGAAGCACAGGTTTGGACCTGATCACAAATGTCCTGGACCCAAAAAGTTGGAAACAAGTTTTTCATTTATGAGTCTTTGGAATATGAGTAATGGTAAAGAAGAGTCAAAACCCAATTTAACTTCAACCACGTCATCTAAGTGGACTTCAAGCCTTCTTGACTTGGCTTCTAAATTGAGTGGGATAGGACAGAGCAGTGGCAGTGGTACTAGTAATAATCAACTGGAGAAGTGTCCACTATGTGATGCTAAGTTTTCCTCGGCCACGAGTTTGGTGGATCATGCGAAAAAAGTTCATCAAAGGAGTGGCGGCAACCAATATGGGGCAAAGAAGGTGTCGATTTCTGCATGCCCCAAGTGCAGTAAAGGTTTTCTGGACCCAGTTTCCCTTGTAGAGCATGTTGAGAGGGATCATGGTGGTTGTTCTTGA
Protein sequence

>Msa1114960

MGTPEFPDLGKHCSVSDCRLIDFLPFTCDRCNQVYCLGHRSYIEHNCTKPNKQDVTVVICPLCAKGVRLIPDQDPNIVWERHVNTDCDPSNYEKATKKKKCPAPGCRETLVFSNTIKCRDCEVDHCLKHRFGPDHKCPGPKKLETSFSFMSLWNMSNGKEESKPNLTSTTSSKWTSSLLDLASKLSGIGQSSGSGTSNNQLEKCPLCDAKFSSATSLVDHAKKVHQRSGGNQYGAKKVSISACPKCSKGFLDPVSLVEHVERDHGGCS*