AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1163290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163290 MtrunA17_Chr7g0260061 93.220 177 11 1 1 177 1 176 7.39e-105 298
Msa1163290 MtrunA17_Chr5g0395001 46.409 181 83 6 1 180 1 168 8.88e-39 130
Msa1163290 MtrunA17_Chr1g0205201 45.143 175 63 6 1 175 1 142 2.02e-36 124
Msa1163290 MtrunA17_Chr1g0205181 43.077 130 62 4 6 129 4 127 4.17e-28 102
Msa1163290 MtrunA17_Chr1g0205191 56.627 83 32 1 28 110 25 103 1.15e-25 95.5
Msa1163290 MtrunA17_Chr1g0185321 42.614 176 83 5 12 171 8 181 3.83e-20 83.2
Msa1163290 MtrunA17_Chr1g0201291 50.649 77 34 2 1 77 1 73 4.11e-16 69.7
Msa1163290 MtrunA17_Chr3g0122441 28.947 114 78 2 1 111 1 114 1.87e-13 65.5
Msa1163290 MtrunA17_Chr8g0356191 29.825 171 106 4 13 172 11 178 3.57e-13 64.7
Msa1163290 MtrunA17_Chr6g0484651 36.782 87 51 2 27 113 39 121 4.99e-12 60.1
Msa1163290 MtrunA17_Chr8g0378461 32.323 99 64 2 5 101 8 105 5.08e-12 61.6
Msa1163290 MtrunA17_Chr3g0118471 32.456 114 72 4 28 137 47 159 6.03e-12 61.6
Msa1163290 MtrunA17_Chr5g0429901 37.755 98 55 4 30 124 34 128 2.72e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163290 AT3G22600.1 50.000 106 51 1 5 110 2 105 3.14e-32 114
Msa1163290 AT2G48130.2 41.279 172 96 4 10 179 9 177 9.56e-31 110
Msa1163290 AT2G48130.6 41.279 172 96 4 10 179 9 177 9.56e-31 110
Msa1163290 AT2G48130.4 41.279 172 96 4 10 179 9 177 9.56e-31 110
Msa1163290 AT2G48130.5 41.279 172 96 4 10 179 9 177 9.56e-31 110
Msa1163290 AT2G48130.1 41.279 172 96 4 10 179 9 177 9.56e-31 110
Msa1163290 AT2G48130.3 41.279 172 96 4 10 179 9 177 9.56e-31 110
Msa1163290 AT4G14815.1 38.462 156 78 4 10 157 5 150 1.31e-28 104
Msa1163290 AT1G03103.1 37.209 172 101 4 8 175 1 169 8.96e-23 89.7
Msa1163290 AT2G13820.2 32.456 114 71 3 12 123 8 117 3.18e-14 66.2
Msa1163290 AT5G09370.2 34.615 104 61 3 29 130 26 124 4.09e-14 66.2
Msa1163290 AT2G13820.1 31.765 170 101 6 12 178 8 165 4.82e-14 67.0
Msa1163290 AT2G13820.3 31.765 170 101 6 12 178 20 177 5.00e-14 67.0
Msa1163290 AT5G09370.1 32.500 120 70 3 29 146 26 136 5.53e-14 66.6
Msa1163290 AT5G09370.3 36.170 94 53 3 29 120 26 114 1.84e-13 65.1
Msa1163290 AT1G55260.1 26.829 164 108 4 24 180 69 227 3.44e-13 65.9
Msa1163290 AT2G44290.1 29.310 174 105 6 1 158 1 172 4.92e-13 65.1
Msa1163290 AT1G55260.2 35.632 87 55 1 24 110 69 154 1.19e-12 64.3
Msa1163290 AT1G27950.1 28.767 146 97 4 29 168 33 177 2.43e-12 62.8
Msa1163290 AT2G44300.1 29.240 171 103 6 26 180 30 198 2.95e-12 62.8
Msa1163290 AT3G43720.1 30.178 169 78 6 30 180 37 183 5.68e-12 62.0
Msa1163290 AT5G64080.2 36.283 113 63 5 30 136 42 151 7.89e-12 61.2
Msa1163290 AT3G43720.2 30.178 169 76 6 30 180 37 181 9.30e-12 61.2
Msa1163290 AT1G05450.2 35.556 135 79 4 24 157 26 153 1.17e-11 61.2
Msa1163290 AT5G64080.1 35.043 117 63 5 30 136 42 155 3.89e-11 59.3
Msa1163290 AT1G73890.1 38.235 102 56 3 22 123 21 115 4.26e-11 59.3

Find 63 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGTTATAGTGGCTCTT+AGG 0.198020 7_4:-54477733 Msa1163290:CDS
TCGATACCTGAAGGAGAATT+TGG 0.222100 7_4:+54477227 None:intergenic
GTAGAATTTAGAAAATAATA+AGG 0.271232 7_4:+54476892 None:intergenic
TCAATATTTAAATGCTGAAT+TGG 0.293069 7_4:+54477833 None:intergenic
CTTTGTTATAGTGGCTCTTA+GGG 0.325801 7_4:-54477732 Msa1163290:CDS
TGGTAATATAGCTAGTTTAC+TGG 0.348541 7_4:+54476804 None:intergenic
TCATACACCTTAACCAAGTT+AGG 0.356033 7_4:-54476838 Msa1163290:three_prime_UTR
AAGCATGTTCTTTGTTATAG+TGG 0.356595 7_4:-54477741 Msa1163290:CDS
TTTATTCCCTAACTTGGTTA+AGG 0.357447 7_4:+54476831 None:intergenic
AACATTCCAAATTCTCCTTC+AGG 0.393320 7_4:-54477233 Msa1163290:intron
AGATGTTGTGATATGAGTTT+TGG 0.401057 7_4:+54477800 None:intergenic
TATCAATCAGACTCGTGCTT+TGG 0.424270 7_4:-54477510 Msa1163290:CDS
TAAAATAAAATAAAATTCAA+TGG 0.428300 7_4:+54476626 None:intergenic
CTCGCAGCTTGCCTCTGTGG+TGG 0.433839 7_4:-54477600 Msa1163290:CDS
TAACTTGGTTAAGGTGTATG+AGG 0.454467 7_4:+54476840 None:intergenic
TCTTCTCCTCCGGCTCCGGC+AGG 0.458961 7_4:-54477269 Msa1163290:CDS
TCGCAGCTTGCCTCTGTGGT+GGG 0.459904 7_4:-54477599 Msa1163290:CDS
TGAAGGAGAATTTGGAATGT+TGG 0.466221 7_4:+54477235 None:intergenic
AGGTATTGATAGGAGGTGTT+TGG 0.469368 7_4:+54477465 None:intergenic
AACTACTTCCTCTTCTCCTC+CGG 0.474991 7_4:-54477279 Msa1163290:CDS
TGTCTATGTGAGGTTGTTAA+CGG 0.475417 7_4:-54477563 Msa1163290:CDS
TGCCGGAGCCGGAGGAGAAG+AGG 0.476590 7_4:+54477271 None:intergenic
TTCCTCTTCTCCTCCGGCTC+CGG 0.477605 7_4:-54477273 Msa1163290:CDS
ATACCAGGGCAGGTATTGAT+AGG 0.482221 7_4:+54477455 None:intergenic
GCAATCAGAGGAAGGGGTGG+AGG 0.484623 7_4:+54477625 None:intergenic
AGCAGCTTGTCAAAACTCAA+TGG 0.494232 7_4:-54476677 Msa1163290:three_prime_UTR
TTCAATTACAAAACTCACAT+TGG 0.502325 7_4:+54476784 None:intergenic
GTAGAAACACCTGCCGGAGC+CGG 0.511959 7_4:+54477260 None:intergenic
TGATCTATGATTTCTAACAT+AGG 0.521776 7_4:+54476650 None:intergenic
GCGAGCAGCAATCAGAGGAA+GGG 0.523573 7_4:+54477618 None:intergenic
CGAGCAGCAATCAGAGGAAG+GGG 0.525269 7_4:+54477619 None:intergenic
CGCAGCTTGCCTCTGTGGTG+GGG 0.529701 7_4:-54477598 Msa1163290:CDS
CATACACCTTAACCAAGTTA+GGG 0.531280 7_4:-54476837 Msa1163290:three_prime_UTR
TGCGAGCAGCAATCAGAGGA+AGG 0.534214 7_4:+54477617 None:intergenic
CTGCTCGCAGCTTGCCTCTG+TGG 0.537994 7_4:-54477603 Msa1163290:CDS
GTTTGGATATTGCAAGCCGT+GGG 0.539828 7_4:+54477482 None:intergenic
CCTCCTATCAATACCTGCCC+TGG 0.542121 7_4:-54477458 Msa1163290:intron
TCGTGCTTTGGCGCTCCCCA+CGG 0.552897 7_4:-54477498 Msa1163290:CDS
TATTTAACTCATACCAGGGC+AGG 0.556069 7_4:+54477445 None:intergenic
GCAGCAATCAGAGGAAGGGG+TGG 0.563387 7_4:+54477622 None:intergenic
TGTTTGGATATTGCAAGCCG+TGG 0.574059 7_4:+54477481 None:intergenic
CCGTGCCTTGACTACATCAC+AGG 0.585027 7_4:-54477653 Msa1163290:CDS
GGTAATATAGCTAGTTTACT+GGG 0.591255 7_4:+54476805 None:intergenic
TTTGTTATAGTGGCTCTTAG+GGG 0.600125 7_4:-54477731 Msa1163290:CDS
ATCACAACATCTTAAAACAA+TGG 0.601319 7_4:-54477789 Msa1163290:exon
TCTGTCCTGTGATGTAGTCA+AGG 0.605452 7_4:+54477648 None:intergenic
GAAACACCTGCCGGAGCCGG+AGG 0.607055 7_4:+54477263 None:intergenic
ACATCAACAACAGGAAGCAG+TGG 0.613629 7_4:-54477005 Msa1163290:CDS
AACTTGGTTAAGGTGTATGA+GGG 0.616814 7_4:+54476841 None:intergenic
CCAGGGCAGGTATTGATAGG+AGG 0.617201 7_4:+54477458 None:intergenic
GGCTCTTAGGGGTGTCACAG+TGG 0.619441 7_4:-54477720 Msa1163290:CDS
AAGCTGCGAGCAGCAATCAG+AGG 0.634741 7_4:+54477613 None:intergenic
ACAACCTCACATAGACATTG+TGG 0.636699 7_4:+54477569 None:intergenic
AGAAAGACTTATGAGCACAT+TGG 0.643132 7_4:+54477676 None:intergenic
CCTGTGATGTAGTCAAGGCA+CGG 0.643394 7_4:+54477653 None:intergenic
TTGGAAGTAGAAACACCTGC+CGG 0.660490 7_4:+54477254 None:intergenic
GGAAGCAGTGGAAGTATCCG+TGG 0.661067 7_4:-54476993 Msa1163290:CDS
TTTGGATATTGCAAGCCGTG+GGG 0.685342 7_4:+54477483 None:intergenic
TATAAGATGAGGTTGAGCCA+CGG 0.687152 7_4:+54476976 None:intergenic
ACAGCCACAATGTCTATGTG+AGG 0.695283 7_4:-54477573 Msa1163290:CDS
ACTTGATGTTGTATAAGATG+AGG 0.718573 7_4:+54476965 None:intergenic
CTATGTGAGGTTGTTAACGG+CGG 0.729400 7_4:-54477560 Msa1163290:CDS
TGGCTGTGACCCCACCACAG+AGG 0.752530 7_4:+54477589 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTACATACTACATATTTTA+TGG + chr7_4:54477121-54477140 None:intergenic 15.0%
!! GTAGAATTTAGAAAATAATA+AGG + chr7_4:54477580-54477599 None:intergenic 15.0%
!!! ATTACATACTACATATTTTA+TGG + chr7_4:54477121-54477140 None:intergenic 15.0%
!! GTAGAATTTAGAAAATAATA+AGG + chr7_4:54477580-54477599 None:intergenic 15.0%
!! TCAATATTTAAATGCTGAAT+TGG + chr7_4:54476639-54476658 None:intergenic 20.0%
!!! AATTTGATGCTTTTTGATAT+AGG - chr7_4:54477434-54477453 Msa1163290:intron 20.0%
!!! ATTTGATGCTTTTTGATATA+GGG - chr7_4:54477435-54477454 Msa1163290:intron 20.0%
!! TCAATATTTAAATGCTGAAT+TGG + chr7_4:54476639-54476658 None:intergenic 20.0%
!!! AATTTGATGCTTTTTGATAT+AGG - chr7_4:54477434-54477453 Msa1163290:intron 20.0%
!!! ATTTGATGCTTTTTGATATA+GGG - chr7_4:54477435-54477454 Msa1163290:intron 20.0%
! ATCACAACATCTTAAAACAA+TGG - chr7_4:54476680-54476699 Msa1163290:three_prime_UTR 25.0%
! TATGTAATGTAATTGAGAGA+TGG - chr7_4:54477133-54477152 Msa1163290:intron 25.0%
! ATGTAATGTAATTGAGAGAT+GGG - chr7_4:54477134-54477153 Msa1163290:intron 25.0%
! TTCAATTACAAAACTCACAT+TGG + chr7_4:54477688-54477707 None:intergenic 25.0%
! TGATCTATGATTTCTAACAT+AGG + chr7_4:54477822-54477841 None:intergenic 25.0%
! ATCACAACATCTTAAAACAA+TGG - chr7_4:54476680-54476699 Msa1163290:three_prime_UTR 25.0%
! TATGTAATGTAATTGAGAGA+TGG - chr7_4:54477133-54477152 Msa1163290:intron 25.0%
! ATGTAATGTAATTGAGAGAT+GGG - chr7_4:54477134-54477153 Msa1163290:intron 25.0%
! TTCAATTACAAAACTCACAT+TGG + chr7_4:54477688-54477707 None:intergenic 25.0%
! TGATCTATGATTTCTAACAT+AGG + chr7_4:54477822-54477841 None:intergenic 25.0%
! AGATGTTGTGATATGAGTTT+TGG + chr7_4:54476672-54476691 None:intergenic 30.0%
! AAGCATGTTCTTTGTTATAG+TGG - chr7_4:54476728-54476747 Msa1163290:three_prime_UTR 30.0%
AACGTATTTAACTCATACCA+GGG + chr7_4:54477031-54477050 None:intergenic 30.0%
AAACGTATTTAACTCATACC+AGG + chr7_4:54477032-54477051 None:intergenic 30.0%
TTTATGGCAAAAGTTAGTAG+CGG + chr7_4:54477105-54477124 None:intergenic 30.0%
! TAATTCCTTGTTGTGATTTC+AGG - chr7_4:54477164-54477183 Msa1163290:intron 30.0%
!!! TTTTTTAGTTCGATACCTGA+AGG + chr7_4:54477254-54477273 None:intergenic 30.0%
ACAAGAATCTATGTGACAAA+TGG - chr7_4:54477383-54477402 Msa1163290:intron 30.0%
ACTTGATGTTGTATAAGATG+AGG + chr7_4:54477507-54477526 None:intergenic 30.0%
!!! ACAATGTTCTTTGCTTTTTC+TGG - chr7_4:54477538-54477557 Msa1163290:CDS 30.0%
TTTATTCCCTAACTTGGTTA+AGG + chr7_4:54477641-54477660 None:intergenic 30.0%
! GGTAATATAGCTAGTTTACT+GGG + chr7_4:54477667-54477686 None:intergenic 30.0%
! TGGTAATATAGCTAGTTTAC+TGG + chr7_4:54477668-54477687 None:intergenic 30.0%
! AGATGTTGTGATATGAGTTT+TGG + chr7_4:54476672-54476691 None:intergenic 30.0%
! AAGCATGTTCTTTGTTATAG+TGG - chr7_4:54476728-54476747 Msa1163290:three_prime_UTR 30.0%
AACGTATTTAACTCATACCA+GGG + chr7_4:54477031-54477050 None:intergenic 30.0%
AAACGTATTTAACTCATACC+AGG + chr7_4:54477032-54477051 None:intergenic 30.0%
TTTATGGCAAAAGTTAGTAG+CGG + chr7_4:54477105-54477124 None:intergenic 30.0%
! TAATTCCTTGTTGTGATTTC+AGG - chr7_4:54477164-54477183 Msa1163290:intron 30.0%
!!! TTTTTTAGTTCGATACCTGA+AGG + chr7_4:54477254-54477273 None:intergenic 30.0%
ACAAGAATCTATGTGACAAA+TGG - chr7_4:54477383-54477402 Msa1163290:intron 30.0%
ACTTGATGTTGTATAAGATG+AGG + chr7_4:54477507-54477526 None:intergenic 30.0%
!!! ACAATGTTCTTTGCTTTTTC+TGG - chr7_4:54477538-54477557 Msa1163290:CDS 30.0%
TTTATTCCCTAACTTGGTTA+AGG + chr7_4:54477641-54477660 None:intergenic 30.0%
! GGTAATATAGCTAGTTTACT+GGG + chr7_4:54477667-54477686 None:intergenic 30.0%
! TGGTAATATAGCTAGTTTAC+TGG + chr7_4:54477668-54477687 None:intergenic 30.0%
! TCTTTGTTATAGTGGCTCTT+AGG - chr7_4:54476736-54476755 Msa1163290:three_prime_UTR 35.0%
! CTTTGTTATAGTGGCTCTTA+GGG - chr7_4:54476737-54476756 Msa1163290:three_prime_UTR 35.0%
! TTTGTTATAGTGGCTCTTAG+GGG - chr7_4:54476738-54476757 Msa1163290:three_prime_UTR 35.0%
AGAAAGACTTATGAGCACAT+TGG + chr7_4:54476796-54476815 None:intergenic 35.0%
TGTCTATGTGAGGTTGTTAA+CGG - chr7_4:54476906-54476925 Msa1163290:CDS 35.0%
TCTATATGTCTTTCTGCACA+CGG - chr7_4:54477073-54477092 Msa1163290:intron 35.0%
! TGAAGGAGAATTTGGAATGT+TGG + chr7_4:54477237-54477256 None:intergenic 35.0%
AACATTCCAAATTCTCCTTC+AGG - chr7_4:54477236-54477255 Msa1163290:CDS 35.0%
! GGGTATTTTACATCAACAAC+AGG - chr7_4:54477455-54477474 Msa1163290:intron 35.0%
!!! ATGTTCTTTGCTTTTTCTGG+TGG - chr7_4:54477541-54477560 Msa1163290:CDS 35.0%
AACTTGGTTAAGGTGTATGA+GGG + chr7_4:54477631-54477650 None:intergenic 35.0%
TAACTTGGTTAAGGTGTATG+AGG + chr7_4:54477632-54477651 None:intergenic 35.0%
TCATACACCTTAACCAAGTT+AGG - chr7_4:54477631-54477650 Msa1163290:CDS 35.0%
CATACACCTTAACCAAGTTA+GGG - chr7_4:54477632-54477651 Msa1163290:CDS 35.0%
! TCTTTGTTATAGTGGCTCTT+AGG - chr7_4:54476736-54476755 Msa1163290:three_prime_UTR 35.0%
! CTTTGTTATAGTGGCTCTTA+GGG - chr7_4:54476737-54476756 Msa1163290:three_prime_UTR 35.0%
! TTTGTTATAGTGGCTCTTAG+GGG - chr7_4:54476738-54476757 Msa1163290:three_prime_UTR 35.0%
AGAAAGACTTATGAGCACAT+TGG + chr7_4:54476796-54476815 None:intergenic 35.0%
TGTCTATGTGAGGTTGTTAA+CGG - chr7_4:54476906-54476925 Msa1163290:CDS 35.0%
TCTATATGTCTTTCTGCACA+CGG - chr7_4:54477073-54477092 Msa1163290:intron 35.0%
! TGAAGGAGAATTTGGAATGT+TGG + chr7_4:54477237-54477256 None:intergenic 35.0%
AACATTCCAAATTCTCCTTC+AGG - chr7_4:54477236-54477255 Msa1163290:CDS 35.0%
! GGGTATTTTACATCAACAAC+AGG - chr7_4:54477455-54477474 Msa1163290:intron 35.0%
!!! ATGTTCTTTGCTTTTTCTGG+TGG - chr7_4:54477541-54477560 Msa1163290:CDS 35.0%
AACTTGGTTAAGGTGTATGA+GGG + chr7_4:54477631-54477650 None:intergenic 35.0%
TAACTTGGTTAAGGTGTATG+AGG + chr7_4:54477632-54477651 None:intergenic 35.0%
TCATACACCTTAACCAAGTT+AGG - chr7_4:54477631-54477650 Msa1163290:CDS 35.0%
CATACACCTTAACCAAGTTA+GGG - chr7_4:54477632-54477651 Msa1163290:CDS 35.0%
ACAACCTCACATAGACATTG+TGG + chr7_4:54476903-54476922 None:intergenic 40.0%
TATCAATCAGACTCGTGCTT+TGG - chr7_4:54476959-54476978 Msa1163290:CDS 40.0%
!! AGGTATTGATAGGAGGTGTT+TGG + chr7_4:54477007-54477026 None:intergenic 40.0%
TATTTAACTCATACCAGGGC+AGG + chr7_4:54477027-54477046 None:intergenic 40.0%
TTGAGCCTGAAATCACAACA+AGG + chr7_4:54477172-54477191 None:intergenic 40.0%
TCGATACCTGAAGGAGAATT+TGG + chr7_4:54477245-54477264 None:intergenic 40.0%
!! TCCCTAGCTTTAGATCATTG+AGG - chr7_4:54477307-54477326 Msa1163290:intron 40.0%
! CCCTCAATGATCTAAAGCTA+GGG + chr7_4:54477311-54477330 None:intergenic 40.0%
!! CCCTAGCTTTAGATCATTGA+GGG - chr7_4:54477308-54477327 Msa1163290:intron 40.0%
! ACCCTCAATGATCTAAAGCT+AGG + chr7_4:54477312-54477331 None:intergenic 40.0%
TATAAGATGAGGTTGAGCCA+CGG + chr7_4:54477496-54477515 None:intergenic 40.0%
GGTGTATGAGGGAAAAAAAG+AGG + chr7_4:54477620-54477639 None:intergenic 40.0%
! GGGACTTTTATTCCCTAACT+TGG + chr7_4:54477647-54477666 None:intergenic 40.0%
AGCAGCTTGTCAAAACTCAA+TGG - chr7_4:54477792-54477811 Msa1163290:exon 40.0%
ACAACCTCACATAGACATTG+TGG + chr7_4:54476903-54476922 None:intergenic 40.0%
TATCAATCAGACTCGTGCTT+TGG - chr7_4:54476959-54476978 Msa1163290:CDS 40.0%
!! AGGTATTGATAGGAGGTGTT+TGG + chr7_4:54477007-54477026 None:intergenic 40.0%
TATTTAACTCATACCAGGGC+AGG + chr7_4:54477027-54477046 None:intergenic 40.0%
TTGAGCCTGAAATCACAACA+AGG + chr7_4:54477172-54477191 None:intergenic 40.0%
TCGATACCTGAAGGAGAATT+TGG + chr7_4:54477245-54477264 None:intergenic 40.0%
!! TCCCTAGCTTTAGATCATTG+AGG - chr7_4:54477307-54477326 Msa1163290:intron 40.0%
! CCCTCAATGATCTAAAGCTA+GGG + chr7_4:54477311-54477330 None:intergenic 40.0%
!! CCCTAGCTTTAGATCATTGA+GGG - chr7_4:54477308-54477327 Msa1163290:intron 40.0%
! ACCCTCAATGATCTAAAGCT+AGG + chr7_4:54477312-54477331 None:intergenic 40.0%
TATAAGATGAGGTTGAGCCA+CGG + chr7_4:54477496-54477515 None:intergenic 40.0%
GGTGTATGAGGGAAAAAAAG+AGG + chr7_4:54477620-54477639 None:intergenic 40.0%
! GGGACTTTTATTCCCTAACT+TGG + chr7_4:54477647-54477666 None:intergenic 40.0%
AGCAGCTTGTCAAAACTCAA+TGG - chr7_4:54477792-54477811 Msa1163290:exon 40.0%
TCTGTCCTGTGATGTAGTCA+AGG + chr7_4:54476824-54476843 None:intergenic 45.0%
ACAGCCACAATGTCTATGTG+AGG - chr7_4:54476896-54476915 Msa1163290:exon 45.0%
CTATGTGAGGTTGTTAACGG+CGG - chr7_4:54476909-54476928 Msa1163290:CDS 45.0%
TTTGGATATTGCAAGCCGTG+GGG + chr7_4:54476989-54477008 None:intergenic 45.0%
GTTTGGATATTGCAAGCCGT+GGG + chr7_4:54476990-54477009 None:intergenic 45.0%
TGTTTGGATATTGCAAGCCG+TGG + chr7_4:54476991-54477010 None:intergenic 45.0%
!! ATACCAGGGCAGGTATTGAT+AGG + chr7_4:54477017-54477036 None:intergenic 45.0%
AACTACTTCCTCTTCTCCTC+CGG - chr7_4:54477190-54477209 Msa1163290:intron 45.0%
TTGGAAGTAGAAACACCTGC+CGG + chr7_4:54477218-54477237 None:intergenic 45.0%
ACATCAACAACAGGAAGCAG+TGG - chr7_4:54477464-54477483 Msa1163290:CDS 45.0%
!! GTTTTGACAAGCTGCTAAGC+AGG + chr7_4:54477788-54477807 None:intergenic 45.0%
TCTGTCCTGTGATGTAGTCA+AGG + chr7_4:54476824-54476843 None:intergenic 45.0%
ACAGCCACAATGTCTATGTG+AGG - chr7_4:54476896-54476915 Msa1163290:exon 45.0%
CTATGTGAGGTTGTTAACGG+CGG - chr7_4:54476909-54476928 Msa1163290:CDS 45.0%
TTTGGATATTGCAAGCCGTG+GGG + chr7_4:54476989-54477008 None:intergenic 45.0%
GTTTGGATATTGCAAGCCGT+GGG + chr7_4:54476990-54477009 None:intergenic 45.0%
TGTTTGGATATTGCAAGCCG+TGG + chr7_4:54476991-54477010 None:intergenic 45.0%
!! ATACCAGGGCAGGTATTGAT+AGG + chr7_4:54477017-54477036 None:intergenic 45.0%
AACTACTTCCTCTTCTCCTC+CGG - chr7_4:54477190-54477209 Msa1163290:intron 45.0%
TTGGAAGTAGAAACACCTGC+CGG + chr7_4:54477218-54477237 None:intergenic 45.0%
ACATCAACAACAGGAAGCAG+TGG - chr7_4:54477464-54477483 Msa1163290:CDS 45.0%
!! GTTTTGACAAGCTGCTAAGC+AGG + chr7_4:54477788-54477807 None:intergenic 45.0%
!! TAAAATAAAATAAAATTCAA+TGG + chr7_4:54477846-54477865 None:intergenic 5.0%
!! TAAAATAAAATAAAATTCAA+TGG + chr7_4:54477846-54477865 None:intergenic 5.0%
CCTGTGATGTAGTCAAGGCA+CGG + chr7_4:54476819-54476838 None:intergenic 50.0%
CCTGTGATGTAGTCAAGGCA+CGG + chr7_4:54476819-54476838 None:intergenic 50.0%
CCGTGCCTTGACTACATCAC+AGG - chr7_4:54476816-54476835 Msa1163290:three_prime_UTR 55.0%
CGAGCAGCAATCAGAGGAAG+GGG + chr7_4:54476853-54476872 None:intergenic 55.0%
GCGAGCAGCAATCAGAGGAA+GGG + chr7_4:54476854-54476873 None:intergenic 55.0%
TGCGAGCAGCAATCAGAGGA+AGG + chr7_4:54476855-54476874 None:intergenic 55.0%
AAGCTGCGAGCAGCAATCAG+AGG + chr7_4:54476859-54476878 None:intergenic 55.0%
!! CCAGGGCAGGTATTGATAGG+AGG + chr7_4:54477014-54477033 None:intergenic 55.0%
CCTCCTATCAATACCTGCCC+TGG - chr7_4:54477011-54477030 Msa1163290:CDS 55.0%
!! GGAAGCAGTGGAAGTATCCG+TGG - chr7_4:54477476-54477495 Msa1163290:CDS 55.0%
CCGTGCCTTGACTACATCAC+AGG - chr7_4:54476816-54476835 Msa1163290:three_prime_UTR 55.0%
CGAGCAGCAATCAGAGGAAG+GGG + chr7_4:54476853-54476872 None:intergenic 55.0%
GCGAGCAGCAATCAGAGGAA+GGG + chr7_4:54476854-54476873 None:intergenic 55.0%
TGCGAGCAGCAATCAGAGGA+AGG + chr7_4:54476855-54476874 None:intergenic 55.0%
AAGCTGCGAGCAGCAATCAG+AGG + chr7_4:54476859-54476878 None:intergenic 55.0%
!! CCAGGGCAGGTATTGATAGG+AGG + chr7_4:54477014-54477033 None:intergenic 55.0%
CCTCCTATCAATACCTGCCC+TGG - chr7_4:54477011-54477030 Msa1163290:CDS 55.0%
!! GGAAGCAGTGGAAGTATCCG+TGG - chr7_4:54477476-54477495 Msa1163290:CDS 55.0%
!! GGCTCTTAGGGGTGTCACAG+TGG - chr7_4:54476749-54476768 Msa1163290:three_prime_UTR 60.0%
GCAATCAGAGGAAGGGGTGG+AGG + chr7_4:54476847-54476866 None:intergenic 60.0%
GCAGCAATCAGAGGAAGGGG+TGG + chr7_4:54476850-54476869 None:intergenic 60.0%
TCGCAGCTTGCCTCTGTGGT+GGG - chr7_4:54476870-54476889 Msa1163290:three_prime_UTR 60.0%
TTCCTCTTCTCCTCCGGCTC+CGG - chr7_4:54477196-54477215 Msa1163290:intron 60.0%
GTAGAAACACCTGCCGGAGC+CGG + chr7_4:54477212-54477231 None:intergenic 60.0%
!! GGCTCTTAGGGGTGTCACAG+TGG - chr7_4:54476749-54476768 Msa1163290:three_prime_UTR 60.0%
GCAATCAGAGGAAGGGGTGG+AGG + chr7_4:54476847-54476866 None:intergenic 60.0%
GCAGCAATCAGAGGAAGGGG+TGG + chr7_4:54476850-54476869 None:intergenic 60.0%
TCGCAGCTTGCCTCTGTGGT+GGG - chr7_4:54476870-54476889 Msa1163290:three_prime_UTR 60.0%
TTCCTCTTCTCCTCCGGCTC+CGG - chr7_4:54477196-54477215 Msa1163290:intron 60.0%
GTAGAAACACCTGCCGGAGC+CGG + chr7_4:54477212-54477231 None:intergenic 60.0%
CTGCTCGCAGCTTGCCTCTG+TGG - chr7_4:54476866-54476885 Msa1163290:three_prime_UTR 65.0%
CTCGCAGCTTGCCTCTGTGG+TGG - chr7_4:54476869-54476888 Msa1163290:three_prime_UTR 65.0%
CGCAGCTTGCCTCTGTGGTG+GGG - chr7_4:54476871-54476890 Msa1163290:three_prime_UTR 65.0%
TGGCTGTGACCCCACCACAG+AGG + chr7_4:54476883-54476902 None:intergenic 65.0%
TCGTGCTTTGGCGCTCCCCA+CGG - chr7_4:54476971-54476990 Msa1163290:CDS 65.0%
CTGCTCGCAGCTTGCCTCTG+TGG - chr7_4:54476866-54476885 Msa1163290:three_prime_UTR 65.0%
CTCGCAGCTTGCCTCTGTGG+TGG - chr7_4:54476869-54476888 Msa1163290:three_prime_UTR 65.0%
CGCAGCTTGCCTCTGTGGTG+GGG - chr7_4:54476871-54476890 Msa1163290:three_prime_UTR 65.0%
TGGCTGTGACCCCACCACAG+AGG + chr7_4:54476883-54476902 None:intergenic 65.0%
TCGTGCTTTGGCGCTCCCCA+CGG - chr7_4:54476971-54476990 Msa1163290:CDS 65.0%
TGCCGGAGCCGGAGGAGAAG+AGG + chr7_4:54477201-54477220 None:intergenic 70.0%
TCTTCTCCTCCGGCTCCGGC+AGG - chr7_4:54477200-54477219 Msa1163290:intron 70.0%
GAAACACCTGCCGGAGCCGG+AGG + chr7_4:54477209-54477228 None:intergenic 70.0%
TGCCGGAGCCGGAGGAGAAG+AGG + chr7_4:54477201-54477220 None:intergenic 70.0%
TCTTCTCCTCCGGCTCCGGC+AGG - chr7_4:54477200-54477219 Msa1163290:intron 70.0%
GAAACACCTGCCGGAGCCGG+AGG + chr7_4:54477209-54477228 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 54476608 54477883 54476608 ID=Msa1163290;Name=Msa1163290
chr7_4 mRNA 54476608 54477883 54476608 ID=Msa1163290-mRNA-1;Parent=Msa1163290;Name=Msa1163290-mRNA-1;_AED=0.07;_eAED=0.07;_QI=91|1|1|1|1|1|3|291|180
chr7_4 exon 54476608 54477035 54476608 ID=Msa1163290-mRNA-1:exon:15820;Parent=Msa1163290-mRNA-1
chr7_4 exon 54477234 54477305 54477234 ID=Msa1163290-mRNA-1:exon:15819;Parent=Msa1163290-mRNA-1
chr7_4 exon 54477459 54477883 54477459 ID=Msa1163290-mRNA-1:exon:15818;Parent=Msa1163290-mRNA-1
chr7_4 five_prime_UTR 54477793 54477883 54477793 ID=Msa1163290-mRNA-1:five_prime_utr;Parent=Msa1163290-mRNA-1
chr7_4 CDS 54477459 54477792 54477459 ID=Msa1163290-mRNA-1:cds;Parent=Msa1163290-mRNA-1
chr7_4 CDS 54477234 54477305 54477234 ID=Msa1163290-mRNA-1:cds;Parent=Msa1163290-mRNA-1
chr7_4 CDS 54476899 54477035 54476899 ID=Msa1163290-mRNA-1:cds;Parent=Msa1163290-mRNA-1
chr7_4 three_prime_UTR 54476608 54476898 54476608 ID=Msa1163290-mRNA-1:three_prime_utr;Parent=Msa1163290-mRNA-1
Gene Sequence

>Msa1163290

ATGGCACAAAGAAACATTGAGATGCTGCTAAGCATGTTCTTTGTTATAGTGGCTCTTAGGGGTGTCACAGTGGCACAAACTGACTCAAATTGCGCCAATGTGCTCATAAGTCTTTCTCCGTGCCTTGACTACATCACAGGACAGACCTCCACCCCTTCCTCTGATTGCTGCTCGCAGCTTGCCTCTGTGGTGGGGTCACAGCCACAATGTCTATGTGAGGTTGTTAACGGCGGTGCTTCATCTATTGCTGCTAGTCTTAATATCAATCAGACTCGTGCTTTGGCGCTCCCCACGGCTTGCAATATCCAAACACCTCCTATCAATACCTGCCCTGGCTCAACTACTTCCTCTTCTCCTCCGGCTCCGGCAGGTGTTTCTACTTCCAACATTCCAAATTCTCCTTCAGGGTATTTTACATCAACAACAGGAAGCAGTGGAAGTATCCGTGGCTCAACCTCATCTTATACAACATCAAGTTCTGTGAAATTACAATGTTCTTTGCTTTTTCTGGTGGTTATCTTTGCAAACCTTATTATTTTCTAA
Protein sequence

>Msa1163290

MAQRNIEMLLSMFFVIVALRGVTVAQTDSNCANVLISLSPCLDYITGQTSTPSSDCCSQLASVVGSQPQCLCEVVNGGASSIAASLNINQTRALALPTACNIQTPPINTCPGSTTSSSPPAPAGVSTSNIPNSPSGYFTSTTGSSGSIRGSTSSYTTSSSVKLQCSLLFLVVIFANLIIF*