AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1174350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174350 MtrunA17_Chr8g0343971 96.167 287 11 0 1 287 74 360 0.0 577
Msa1174350 MtrunA17_Chr8g0343981 72.125 287 77 1 1 287 74 357 1.37e-155 438
Msa1174350 MtrunA17_Chr3g0107591 63.390 295 96 2 3 286 75 368 1.67e-140 400
Msa1174350 MtrunA17_Chr8g0372671 64.360 289 99 2 2 287 75 362 1.01e-138 395
Msa1174350 MtrunA17_Chr4g0008111 63.287 286 104 1 2 287 75 359 5.91e-137 391
Msa1174350 MtrunA17_Chr6g0475241 63.860 285 99 3 2 286 76 356 1.75e-130 374
Msa1174350 MtrunA17_Chr5g0404631 58.923 297 109 3 2 286 85 380 5.17e-129 371
Msa1174350 MtrunA17_Chr8g0372821 59.661 295 107 2 2 285 75 368 1.22e-128 370
Msa1174350 MtrunA17_Chr6g0475221 59.932 292 97 4 2 286 75 353 7.64e-124 357
Msa1174350 MtrunA17_Chr3g0144871 39.667 300 162 3 2 287 75 369 1.02e-77 240
Msa1174350 MtrunA17_Chr5g0419851 39.519 291 164 5 2 285 135 420 3.94e-69 220
Msa1174350 MtrunA17_Chr8g0372801 68.939 132 41 0 17 148 31 162 1.55e-67 208
Msa1174350 MtrunA17_Chr3g0107871 39.932 293 162 5 2 285 53 340 1.28e-66 211
Msa1174350 MtrunA17_Chr7g0252361 33.784 296 178 3 2 285 74 363 3.61e-55 182
Msa1174350 MtrunA17_Chr1g0155451 41.627 209 111 3 88 286 9 216 6.82e-53 172
Msa1174350 MtrunA17_Chr3g0100101 35.484 279 152 7 5 263 87 357 1.08e-45 158
Msa1174350 MtrunA17_Chr1g0208641 36.491 285 150 8 13 286 102 366 2.91e-45 156
Msa1174350 MtrunA17_Chr5g0444921 33.003 303 174 8 3 284 95 389 8.66e-43 150
Msa1174350 MtrunA17_Chr7g0252501 37.186 199 109 3 95 285 38 228 2.20e-37 132
Msa1174350 MtrunA17_Chr6g0455071 48.246 114 48 2 64 171 11 119 3.66e-34 120
Msa1174350 MtrunA17_Chr1g0155471 39.316 117 68 2 173 286 8 124 4.77e-22 89.0
Msa1174350 MtrunA17_Chr1g0155481 44.330 97 47 2 83 173 4 99 2.14e-21 88.2
Msa1174350 MtrunA17_Chr3g0099271 30.198 202 121 5 95 284 15 208 1.95e-20 87.0
Msa1174350 MtrunA17_Chr6g0455081 31.452 124 77 1 172 287 8 131 1.99e-16 74.3
Msa1174350 MtrunA17_Chr7g0252341 46.667 75 37 1 14 88 61 132 5.07e-14 68.2
Msa1174350 MtrunA17_Chr3g0099281 44.156 77 40 2 15 88 105 181 1.29e-13 68.2
Msa1174350 MtrunA17_Chr1g0155461 46.753 77 27 2 13 79 90 162 2.38e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174350 AT3G11480.1 41.404 285 164 1 2 286 98 379 6.78e-78 241
Msa1174350 AT5G66430.1 42.808 292 155 3 2 286 68 354 4.71e-76 236
Msa1174350 AT5G04380.2 41.034 290 161 2 2 286 34 318 7.03e-76 234
Msa1174350 AT5G04380.3 41.034 290 161 2 2 286 105 389 6.49e-75 234
Msa1174350 AT5G04370.1 43.206 287 157 3 2 286 108 390 9.91e-75 234
Msa1174350 AT5G04370.2 43.206 287 157 3 2 286 127 409 1.76e-74 234
Msa1174350 AT5G04370.3 43.206 287 157 3 2 286 113 395 2.10e-74 233
Msa1174350 AT4G36470.1 42.652 279 152 3 15 286 93 370 5.66e-72 226
Msa1174350 AT5G38020.2 43.051 295 147 8 8 286 40 329 9.62e-72 224
Msa1174350 AT3G21950.1 42.177 294 151 6 8 286 79 368 1.18e-71 225
Msa1174350 AT5G38020.1 43.051 295 147 8 8 286 79 368 2.09e-71 224
Msa1174350 AT5G04380.1 40.690 290 155 3 2 286 101 378 1.21e-70 223
Msa1174350 AT2G14060.1 41.724 290 154 5 8 286 87 372 3.32e-68 216
Msa1174350 AT3G21950.2 38.344 326 150 7 8 286 79 400 4.43e-66 212
Msa1174350 AT1G19640.1 41.638 293 141 6 2 265 76 367 4.79e-59 193
Msa1174350 AT1G68040.1 35.154 293 167 5 3 286 85 363 1.97e-48 165
Msa1174350 AT5G55250.2 35.106 282 153 8 1 260 47 320 1.35e-46 160
Msa1174350 AT5G55250.1 35.231 281 152 8 2 260 86 358 2.52e-46 160
Msa1174350 AT2G14060.2 47.712 153 76 2 8 160 87 235 1.12e-43 149
Msa1174350 AT1G15125.1 33.984 256 143 6 14 261 83 320 1.82e-42 149
Msa1174350 AT5G38100.3 35.271 258 141 8 16 265 74 313 2.40e-39 140
Msa1174350 AT5G38100.1 35.271 258 141 8 16 265 97 336 3.13e-39 140
Msa1174350 AT5G56300.1 33.824 272 145 8 13 262 103 361 8.42e-39 140
Msa1174350 AT4G26420.1 33.840 263 148 8 16 261 97 350 3.43e-37 135
Msa1174350 AT5G37990.1 32.819 259 152 5 13 265 97 339 1.70e-36 134
Msa1174350 AT5G38780.2 30.451 266 153 7 11 265 45 289 3.80e-36 131
Msa1174350 AT5G37970.1 33.205 259 151 5 13 265 97 339 5.84e-36 132
Msa1174350 AT5G38780.1 30.451 266 153 7 11 265 94 338 1.20e-35 131
Msa1174350 AT3G44840.1 32.677 254 144 8 16 262 89 322 1.86e-31 120
Msa1174350 AT1G66700.1 30.682 264 154 6 16 271 94 336 2.85e-29 114
Msa1174350 AT1G66720.1 30.364 247 150 4 16 258 94 322 7.68e-29 113
Msa1174350 AT4G26420.3 42.647 136 64 3 16 145 97 224 7.45e-28 108
Msa1174350 AT3G44860.1 33.088 272 144 11 16 273 89 336 9.33e-28 110
Msa1174350 AT3G44870.1 30.996 271 161 6 11 273 81 333 1.06e-27 110
Msa1174350 AT4G26420.2 42.647 136 64 3 16 145 85 212 1.55e-27 107
Msa1174350 AT5G38100.2 43.511 131 57 4 16 140 97 216 1.47e-24 99.4
Msa1174350 AT5G38100.4 43.511 131 57 4 16 140 97 216 1.55e-24 99.4
Msa1174350 AT1G66690.1 29.771 262 159 6 16 271 94 336 2.50e-23 98.2
Msa1174350 AT1G15125.2 32.121 165 92 4 14 171 83 234 6.28e-22 92.4
Msa1174350 AT1G66700.3 34.356 163 89 3 16 173 94 243 2.31e-20 88.6

Find 75 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTATATGTTGTTTATTAA+TGG 0.128488 8_1:-5439122 Msa1174350:three_prime_UTR
ATCTGCAATTGCAAGGCTTC+TGG 0.218678 8_1:+5440287 None:intergenic
TGCTACTTCTTTGGTGTTCC+CGG 0.231997 8_1:-5440054 Msa1174350:CDS
GATGAAAGTGATTCTGAAAA+TGG 0.241048 8_1:-5439335 Msa1174350:CDS
TCATGCAGTTCTTATTATAT+TGG 0.263360 8_1:+5439013 None:intergenic
ATGGGTCCTTGCTACTTCTT+TGG 0.270416 8_1:-5440063 Msa1174350:CDS
GGCTACTGCTCTTAATGATA+TGG 0.272144 8_1:-5439594 Msa1174350:CDS
GTATGCTACAGGGGATTATA+AGG 0.298506 8_1:-5439493 Msa1174350:intron
CGAAGGTATAGATAACAATA+AGG 0.328330 8_1:-5439816 Msa1174350:CDS
TGGAATATTGTTGCGACGAT+TGG 0.341844 8_1:-5439625 Msa1174350:CDS
TGATGAAATCAAAACTGAAA+TGG 0.343051 8_1:-5440082 Msa1174350:CDS
AAGCCTTGCAATTGCAGATC+TGG 0.344973 8_1:-5440283 Msa1174350:five_prime_UTR
AAACAACTCCATGTGATTGA+TGG 0.354713 8_1:+5439382 None:intergenic
TAAATAAATTGAAAGTTGTT+GGG 0.355748 8_1:-5439098 Msa1174350:three_prime_UTR
CTTATTGTTATCTATACCTT+CGG 0.355792 8_1:+5439817 None:intergenic
TCTTAATGATATGGTGTTGC+AGG 0.360149 8_1:-5439585 Msa1174350:intron
ATTGAAAGTTGTTGGGGTAC+TGG 0.368033 8_1:-5439091 Msa1174350:three_prime_UTR
TTAAATAAATTGAAAGTTGT+TGG 0.374634 8_1:-5439099 Msa1174350:three_prime_UTR
TCTGCAATTGCAAGGCTTCT+GGG 0.377937 8_1:+5440288 None:intergenic
CAAGTGTCGCGCAAAGGAAT+TGG 0.382581 8_1:-5439708 Msa1174350:CDS
AACGGAATTCATTCTCTCCT+TGG 0.389981 8_1:+5439193 None:intergenic
GGAATATTGTTGCGACGATT+GGG 0.395406 8_1:-5439624 Msa1174350:CDS
AACTCTATCATAGAAAGAAC+CGG 0.395889 8_1:+5440035 None:intergenic
GAGATGCATATTTCAATTGT+TGG 0.419986 8_1:+5440193 None:intergenic
CAATGATATGGTAACATTAA+CGG 0.422223 8_1:+5439175 None:intergenic
GATGAAATCAAAACTGAAAT+GGG 0.433507 8_1:-5440081 Msa1174350:CDS
GAAAATGGATATGATGTTGC+AGG 0.441689 8_1:-5439320 Msa1174350:CDS
TTTATGTCATTATAGGGAAA+GGG 0.443756 8_1:-5440372 Msa1174350:five_prime_UTR
ACTTCCAACTCCACTTCATT+CGG 0.444785 8_1:+5439425 None:intergenic
CGCAAAGGAATTGGTTGAAG+GGG 0.459467 8_1:-5439699 Msa1174350:CDS
TTATTGTTATCTATACCTTC+GGG 0.460263 8_1:+5439818 None:intergenic
CCTTTGCTAGTTAGTCACTT+TGG 0.468922 8_1:-5439275 Msa1174350:CDS
AACACAACAACGCAGACAAT+TGG 0.469150 8_1:+5438970 None:intergenic
TAAGCCTTGACGACATTTGA+GGG 0.478306 8_1:+5439767 None:intergenic
GAAGGTATAGATAACAATAA+GGG 0.479026 8_1:-5439815 Msa1174350:CDS
CTCAGAAATCATCATGGTTG+TGG 0.481895 8_1:-5440226 Msa1174350:exon
CGCGCAAAGGAATTGGTTGA+AGG 0.488936 8_1:-5439701 Msa1174350:CDS
AAATAAATTGAAAGTTGTTG+GGG 0.509344 8_1:-5439097 Msa1174350:three_prime_UTR
ACGAAATGCAGACTTCGATC+TGG 0.517231 8_1:+5440009 None:intergenic
AGCCTTGCAATTGCAGATCT+GGG 0.518124 8_1:-5440282 Msa1174350:five_prime_UTR
GCAGATCTGGGTTGTTCTTG+TGG 0.519811 8_1:-5440270 Msa1174350:five_prime_UTR
GTAAGCCTTGACGACATTTG+AGG 0.520325 8_1:+5439766 None:intergenic
CAACAATATTCCACGTCACA+TGG 0.526509 8_1:+5439635 None:intergenic
CGCATGGTTCTAACGATGGT+GGG 0.533146 8_1:-5439674 Msa1174350:CDS
CAGTCTTCACTGGCTATCAA+AGG 0.539238 8_1:-5439974 Msa1174350:intron
TATGCTACAGGGGATTATAA+GGG 0.539471 8_1:-5439492 Msa1174350:intron
AAGGCTTCTGGGAAGTATGC+TGG 0.539921 8_1:+5440299 None:intergenic
GCTGGAGTAGAGGCTAGTTA+TGG 0.545444 8_1:+5440317 None:intergenic
TTATGTCATTATAGGGAAAG+GGG 0.554192 8_1:-5440371 Msa1174350:five_prime_UTR
ACTGCTCTTACAGTCTTCAC+TGG 0.558182 8_1:-5439984 Msa1174350:CDS
TGGTGAAGCTATCATTGAAG+AGG 0.568437 8_1:-5439255 Msa1174350:CDS
ATTGATTGATCAAATATCCA+AGG 0.570539 8_1:-5439210 Msa1174350:CDS
GCGCAAAGGAATTGGTTGAA+GGG 0.574318 8_1:-5439700 Msa1174350:CDS
AACTCCACTTCATTCGGAGA+CGG 0.584106 8_1:+5439431 None:intergenic
CAATCAATAATTCCTTGTAA+CGG 0.589742 8_1:+5439225 None:intergenic
CCAAAGTGACTAACTAGCAA+AGG 0.593693 8_1:+5439275 None:intergenic
ATCGTTTGCCATCAATCACA+TGG 0.595409 8_1:-5439390 Msa1174350:CDS
GAGTTGGAAGTTCTCAATGA+AGG 0.596864 8_1:-5439413 Msa1174350:CDS
TTATCCGTCTCCGAATGAAG+TGG 0.599970 8_1:-5439435 Msa1174350:CDS
TGGGAAGTATGCTGGAGTAG+AGG 0.601219 8_1:+5440307 None:intergenic
AACCCAGATCTGCAATTGCA+AGG 0.605454 8_1:+5440280 None:intergenic
TGCAGGGTGCATTAGAGCTG+TGG 0.607342 8_1:-5439303 Msa1174350:CDS
AGTGAAGACTGTAAGAGCAG+TGG 0.607547 8_1:+5439986 None:intergenic
TCGCATGGTTCTAACGATGG+TGG 0.615198 8_1:-5439675 Msa1174350:CDS
TTGGTGAATTAGGTTCCCGA+AGG 0.623427 8_1:-5439833 Msa1174350:intron
GGAACACCAAAGAAGTAGCA+AGG 0.627551 8_1:+5440057 None:intergenic
AAGTCCCTCAAATGTCGTCA+AGG 0.632935 8_1:-5439771 Msa1174350:CDS
TTCGGAGACGGATAATAGTG+AGG 0.637717 8_1:+5439443 None:intergenic
TCGGAGACGGATAATAGTGA+GGG 0.639677 8_1:+5439444 None:intergenic
AAATGAAGATCCATGTGACG+TGG 0.640046 8_1:-5439645 Msa1174350:CDS
ACTCTATCATAGAAAGAACC+GGG 0.650635 8_1:+5440036 None:intergenic
TGTTGTCTCAGAAATCATCA+TGG 0.661673 8_1:-5440232 Msa1174350:exon
GGGTCGCATGGTTCTAACGA+TGG 0.671204 8_1:-5439678 Msa1174350:CDS
GCAAAGGAATTGGTTGAAGG+GGG 0.680145 8_1:-5439698 Msa1174350:CDS
AAAATGGATATGATGTTGCA+GGG 0.702180 8_1:-5439319 Msa1174350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAGAAAATATTTATCA+CGG + chr8_1:5439040-5439059 None:intergenic 10.0%
!! TAAAAAGAAAATATTTATCA+CGG + chr8_1:5439040-5439059 None:intergenic 10.0%
!!! TTTTCTTTTTATGTCATTAT+AGG - chr8_1:5439049-5439068 Msa1174350:three_prime_UTR 15.0%
!!! TTTCTTTTTATGTCATTATA+GGG - chr8_1:5439050-5439069 Msa1174350:three_prime_UTR 15.0%
!! ATTAAAAAAGATCTTGTATT+CGG + chr8_1:5439261-5439280 None:intergenic 15.0%
!!! CTAATTGATTTTTATTTGTT+TGG - chr8_1:5439576-5439595 Msa1174350:intron 15.0%
!!! ATATAACTCATTTTTCATAT+AGG + chr8_1:5439884-5439903 None:intergenic 15.0%
!! ATATGAAAAATGAGTTATAT+AGG - chr8_1:5439884-5439903 Msa1174350:intron 15.0%
!!! CATTATATGTTGTTTATTAA+TGG - chr8_1:5440306-5440325 Msa1174350:five_prime_UTR 15.0%
!! TTAAATAAATTGAAAGTTGT+TGG - chr8_1:5440329-5440348 Msa1174350:five_prime_UTR 15.0%
!! TAAATAAATTGAAAGTTGTT+GGG - chr8_1:5440330-5440349 Msa1174350:five_prime_UTR 15.0%
!!! TTTTCTTTTTATGTCATTAT+AGG - chr8_1:5439049-5439068 Msa1174350:three_prime_UTR 15.0%
!!! TTTCTTTTTATGTCATTATA+GGG - chr8_1:5439050-5439069 Msa1174350:three_prime_UTR 15.0%
!! ATTAAAAAAGATCTTGTATT+CGG + chr8_1:5439261-5439280 None:intergenic 15.0%
!!! CTAATTGATTTTTATTTGTT+TGG - chr8_1:5439576-5439595 Msa1174350:intron 15.0%
!!! ATATAACTCATTTTTCATAT+AGG + chr8_1:5439884-5439903 None:intergenic 15.0%
!! ATATGAAAAATGAGTTATAT+AGG - chr8_1:5439884-5439903 Msa1174350:intron 15.0%
!!! CATTATATGTTGTTTATTAA+TGG - chr8_1:5440306-5440325 Msa1174350:five_prime_UTR 15.0%
!! TTAAATAAATTGAAAGTTGT+TGG - chr8_1:5440329-5440348 Msa1174350:five_prime_UTR 15.0%
!! TAAATAAATTGAAAGTTGTT+GGG - chr8_1:5440330-5440349 Msa1174350:five_prime_UTR 15.0%
!! TTAAAAAAGATCTTGTATTC+GGG + chr8_1:5439260-5439279 None:intergenic 20.0%
!!! GATCTTTTTTAATGATCTTT+CGG - chr8_1:5439268-5439287 Msa1174350:CDS 20.0%
!!! ATCTTTTTTAATGATCTTTC+GGG - chr8_1:5439269-5439288 Msa1174350:CDS 20.0%
!!! TTTTATTTGTTTGGTGAATT+AGG - chr8_1:5439585-5439604 Msa1174350:intron 20.0%
!! AAATAAATTGAAAGTTGTTG+GGG - chr8_1:5440331-5440350 Msa1174350:five_prime_UTR 20.0%
!! TTAAAAAAGATCTTGTATTC+GGG + chr8_1:5439260-5439279 None:intergenic 20.0%
!!! GATCTTTTTTAATGATCTTT+CGG - chr8_1:5439268-5439287 Msa1174350:CDS 20.0%
!!! ATCTTTTTTAATGATCTTTC+GGG - chr8_1:5439269-5439288 Msa1174350:CDS 20.0%
!!! TTTTATTTGTTTGGTGAATT+AGG - chr8_1:5439585-5439604 Msa1174350:intron 20.0%
!! AAATAAATTGAAAGTTGTTG+GGG - chr8_1:5440331-5440350 Msa1174350:five_prime_UTR 20.0%
! GATTGCATTGAAAAAAAGAT+TGG - chr8_1:5438995-5439014 Msa1174350:three_prime_UTR 25.0%
!! TTTTATGTCATTATAGGGAA+AGG - chr8_1:5439055-5439074 Msa1174350:three_prime_UTR 25.0%
! TTTATGTCATTATAGGGAAA+GGG - chr8_1:5439056-5439075 Msa1174350:three_prime_UTR 25.0%
!!! TCTTTTTTAATGATCTTTCG+GGG - chr8_1:5439270-5439289 Msa1174350:CDS 25.0%
! TGATGAAATCAAAACTGAAA+TGG - chr8_1:5439346-5439365 Msa1174350:CDS 25.0%
! GATGAAATCAAAACTGAAAT+GGG - chr8_1:5439347-5439366 Msa1174350:CDS 25.0%
! TTATTGTTATCTATACCTTC+GGG + chr8_1:5439613-5439632 None:intergenic 25.0%
! CTTATTGTTATCTATACCTT+CGG + chr8_1:5439614-5439633 None:intergenic 25.0%
!! GAAGGTATAGATAACAATAA+GGG - chr8_1:5439613-5439632 Msa1174350:CDS 25.0%
! CAATCAATAATTCCTTGTAA+CGG + chr8_1:5440206-5440225 None:intergenic 25.0%
! ATTGATTGATCAAATATCCA+AGG - chr8_1:5440218-5440237 Msa1174350:exon 25.0%
! CAATGATATGGTAACATTAA+CGG + chr8_1:5440256-5440275 None:intergenic 25.0%
!!! TGATTTTCTAGTCAATGATA+TGG + chr8_1:5440268-5440287 None:intergenic 25.0%
! TCATGCAGTTCTTATTATAT+TGG + chr8_1:5440418-5440437 None:intergenic 25.0%
! GATTGCATTGAAAAAAAGAT+TGG - chr8_1:5438995-5439014 Msa1174350:three_prime_UTR 25.0%
!! TTTTATGTCATTATAGGGAA+AGG - chr8_1:5439055-5439074 Msa1174350:three_prime_UTR 25.0%
! TTTATGTCATTATAGGGAAA+GGG - chr8_1:5439056-5439075 Msa1174350:three_prime_UTR 25.0%
!!! TCTTTTTTAATGATCTTTCG+GGG - chr8_1:5439270-5439289 Msa1174350:CDS 25.0%
! TGATGAAATCAAAACTGAAA+TGG - chr8_1:5439346-5439365 Msa1174350:CDS 25.0%
! GATGAAATCAAAACTGAAAT+GGG - chr8_1:5439347-5439366 Msa1174350:CDS 25.0%
! TTATTGTTATCTATACCTTC+GGG + chr8_1:5439613-5439632 None:intergenic 25.0%
! CTTATTGTTATCTATACCTT+CGG + chr8_1:5439614-5439633 None:intergenic 25.0%
!! GAAGGTATAGATAACAATAA+GGG - chr8_1:5439613-5439632 Msa1174350:CDS 25.0%
! CAATCAATAATTCCTTGTAA+CGG + chr8_1:5440206-5440225 None:intergenic 25.0%
! ATTGATTGATCAAATATCCA+AGG - chr8_1:5440218-5440237 Msa1174350:exon 25.0%
! CAATGATATGGTAACATTAA+CGG + chr8_1:5440256-5440275 None:intergenic 25.0%
!!! TGATTTTCTAGTCAATGATA+TGG + chr8_1:5440268-5440287 None:intergenic 25.0%
! TCATGCAGTTCTTATTATAT+TGG + chr8_1:5440418-5440437 None:intergenic 25.0%
TTATGTCATTATAGGGAAAG+GGG - chr8_1:5439057-5439076 Msa1174350:three_prime_UTR 30.0%
GAGATGCATATTTCAATTGT+TGG + chr8_1:5439238-5439257 None:intergenic 30.0%
AACTCTATCATAGAAAGAAC+CGG + chr8_1:5439396-5439415 None:intergenic 30.0%
! CGAAGGTATAGATAACAATA+AGG - chr8_1:5439612-5439631 Msa1174350:CDS 30.0%
TCCTTAATATTGTATGCTAC+AGG - chr8_1:5439924-5439943 Msa1174350:intron 30.0%
CCTTAATATTGTATGCTACA+GGG - chr8_1:5439925-5439944 Msa1174350:intron 30.0%
CTTAATATTGTATGCTACAG+GGG - chr8_1:5439926-5439945 Msa1174350:intron 30.0%
! GATGAAAGTGATTCTGAAAA+TGG - chr8_1:5440093-5440112 Msa1174350:CDS 30.0%
AAAATGGATATGATGTTGCA+GGG - chr8_1:5440109-5440128 Msa1174350:CDS 30.0%
TTATGTCATTATAGGGAAAG+GGG - chr8_1:5439057-5439076 Msa1174350:three_prime_UTR 30.0%
GAGATGCATATTTCAATTGT+TGG + chr8_1:5439238-5439257 None:intergenic 30.0%
AACTCTATCATAGAAAGAAC+CGG + chr8_1:5439396-5439415 None:intergenic 30.0%
! CGAAGGTATAGATAACAATA+AGG - chr8_1:5439612-5439631 Msa1174350:CDS 30.0%
TCCTTAATATTGTATGCTAC+AGG - chr8_1:5439924-5439943 Msa1174350:intron 30.0%
CCTTAATATTGTATGCTACA+GGG - chr8_1:5439925-5439944 Msa1174350:intron 30.0%
CTTAATATTGTATGCTACAG+GGG - chr8_1:5439926-5439945 Msa1174350:intron 30.0%
! GATGAAAGTGATTCTGAAAA+TGG - chr8_1:5440093-5440112 Msa1174350:CDS 30.0%
AAAATGGATATGATGTTGCA+GGG - chr8_1:5440109-5440128 Msa1174350:CDS 30.0%
TGTTGTCTCAGAAATCATCA+TGG - chr8_1:5439196-5439215 Msa1174350:CDS 35.0%
ACTCTATCATAGAAAGAACC+GGG + chr8_1:5439395-5439414 None:intergenic 35.0%
!! TCTTAATGATATGGTGTTGC+AGG - chr8_1:5439843-5439862 Msa1174350:intron 35.0%
CCCTGTAGCATACAATATTA+AGG + chr8_1:5439928-5439947 None:intergenic 35.0%
TATGCTACAGGGGATTATAA+GGG - chr8_1:5439936-5439955 Msa1174350:intron 35.0%
AAACAACTCCATGTGATTGA+TGG + chr8_1:5440049-5440068 None:intergenic 35.0%
GAAAATGGATATGATGTTGC+AGG - chr8_1:5440108-5440127 Msa1174350:CDS 35.0%
TGTTGTCTCAGAAATCATCA+TGG - chr8_1:5439196-5439215 Msa1174350:CDS 35.0%
ACTCTATCATAGAAAGAACC+GGG + chr8_1:5439395-5439414 None:intergenic 35.0%
!! TCTTAATGATATGGTGTTGC+AGG - chr8_1:5439843-5439862 Msa1174350:intron 35.0%
CCCTGTAGCATACAATATTA+AGG + chr8_1:5439928-5439947 None:intergenic 35.0%
TATGCTACAGGGGATTATAA+GGG - chr8_1:5439936-5439955 Msa1174350:intron 35.0%
AAACAACTCCATGTGATTGA+TGG + chr8_1:5440049-5440068 None:intergenic 35.0%
GAAAATGGATATGATGTTGC+AGG - chr8_1:5440108-5440127 Msa1174350:CDS 35.0%
CTCAGAAATCATCATGGTTG+TGG - chr8_1:5439202-5439221 Msa1174350:CDS 40.0%
TAAGCCTTGACGACATTTGA+GGG + chr8_1:5439664-5439683 None:intergenic 40.0%
AAATGAAGATCCATGTGACG+TGG - chr8_1:5439783-5439802 Msa1174350:CDS 40.0%
CAACAATATTCCACGTCACA+TGG + chr8_1:5439796-5439815 None:intergenic 40.0%
TGGAATATTGTTGCGACGAT+TGG - chr8_1:5439803-5439822 Msa1174350:CDS 40.0%
GGAATATTGTTGCGACGATT+GGG - chr8_1:5439804-5439823 Msa1174350:CDS 40.0%
!!! TTGCGACGATTGGGATTTTT+TGG - chr8_1:5439813-5439832 Msa1174350:CDS 40.0%
GGCTACTGCTCTTAATGATA+TGG - chr8_1:5439834-5439853 Msa1174350:intron 40.0%
GTATGCTACAGGGGATTATA+AGG - chr8_1:5439935-5439954 Msa1174350:intron 40.0%
ACTTCCAACTCCACTTCATT+CGG + chr8_1:5440006-5440025 None:intergenic 40.0%
GAGTTGGAAGTTCTCAATGA+AGG - chr8_1:5440015-5440034 Msa1174350:CDS 40.0%
ATCGTTTGCCATCAATCACA+TGG - chr8_1:5440038-5440057 Msa1174350:CDS 40.0%
CCAAAGTGACTAACTAGCAA+AGG + chr8_1:5440156-5440175 None:intergenic 40.0%
CCTTTGCTAGTTAGTCACTT+TGG - chr8_1:5440153-5440172 Msa1174350:CDS 40.0%
TGGTGAAGCTATCATTGAAG+AGG - chr8_1:5440173-5440192 Msa1174350:CDS 40.0%
!!! AGAGGTTTTTAGCCGTTACA+AGG - chr8_1:5440191-5440210 Msa1174350:CDS 40.0%
AACGGAATTCATTCTCTCCT+TGG + chr8_1:5440238-5440257 None:intergenic 40.0%
ATTGAAAGTTGTTGGGGTAC+TGG - chr8_1:5440337-5440356 Msa1174350:five_prime_UTR 40.0%
AACACAACAACGCAGACAAT+TGG + chr8_1:5440461-5440480 None:intergenic 40.0%
CTCAGAAATCATCATGGTTG+TGG - chr8_1:5439202-5439221 Msa1174350:CDS 40.0%
TAAGCCTTGACGACATTTGA+GGG + chr8_1:5439664-5439683 None:intergenic 40.0%
AAATGAAGATCCATGTGACG+TGG - chr8_1:5439783-5439802 Msa1174350:CDS 40.0%
CAACAATATTCCACGTCACA+TGG + chr8_1:5439796-5439815 None:intergenic 40.0%
TGGAATATTGTTGCGACGAT+TGG - chr8_1:5439803-5439822 Msa1174350:CDS 40.0%
GGAATATTGTTGCGACGATT+GGG - chr8_1:5439804-5439823 Msa1174350:CDS 40.0%
!!! TTGCGACGATTGGGATTTTT+TGG - chr8_1:5439813-5439832 Msa1174350:CDS 40.0%
GGCTACTGCTCTTAATGATA+TGG - chr8_1:5439834-5439853 Msa1174350:intron 40.0%
GTATGCTACAGGGGATTATA+AGG - chr8_1:5439935-5439954 Msa1174350:intron 40.0%
ACTTCCAACTCCACTTCATT+CGG + chr8_1:5440006-5440025 None:intergenic 40.0%
GAGTTGGAAGTTCTCAATGA+AGG - chr8_1:5440015-5440034 Msa1174350:CDS 40.0%
ATCGTTTGCCATCAATCACA+TGG - chr8_1:5440038-5440057 Msa1174350:CDS 40.0%
CCAAAGTGACTAACTAGCAA+AGG + chr8_1:5440156-5440175 None:intergenic 40.0%
CCTTTGCTAGTTAGTCACTT+TGG - chr8_1:5440153-5440172 Msa1174350:CDS 40.0%
TGGTGAAGCTATCATTGAAG+AGG - chr8_1:5440173-5440192 Msa1174350:CDS 40.0%
!!! AGAGGTTTTTAGCCGTTACA+AGG - chr8_1:5440191-5440210 Msa1174350:CDS 40.0%
AACGGAATTCATTCTCTCCT+TGG + chr8_1:5440238-5440257 None:intergenic 40.0%
ATTGAAAGTTGTTGGGGTAC+TGG - chr8_1:5440337-5440356 Msa1174350:five_prime_UTR 40.0%
AACACAACAACGCAGACAAT+TGG + chr8_1:5440461-5440480 None:intergenic 40.0%
TCTGCAATTGCAAGGCTTCT+GGG + chr8_1:5439143-5439162 None:intergenic 45.0%
ATCTGCAATTGCAAGGCTTC+TGG + chr8_1:5439144-5439163 None:intergenic 45.0%
AAGCCTTGCAATTGCAGATC+TGG - chr8_1:5439145-5439164 Msa1174350:exon 45.0%
AGCCTTGCAATTGCAGATCT+GGG - chr8_1:5439146-5439165 Msa1174350:exon 45.0%
AACCCAGATCTGCAATTGCA+AGG + chr8_1:5439151-5439170 None:intergenic 45.0%
! ATGGGTCCTTGCTACTTCTT+TGG - chr8_1:5439365-5439384 Msa1174350:CDS 45.0%
GGAACACCAAAGAAGTAGCA+AGG + chr8_1:5439374-5439393 None:intergenic 45.0%
!! TGCTACTTCTTTGGTGTTCC+CGG - chr8_1:5439374-5439393 Msa1174350:CDS 45.0%
ACGAAATGCAGACTTCGATC+TGG + chr8_1:5439422-5439441 None:intergenic 45.0%
AGTGAAGACTGTAAGAGCAG+TGG + chr8_1:5439445-5439464 None:intergenic 45.0%
ACTGCTCTTACAGTCTTCAC+TGG - chr8_1:5439444-5439463 Msa1174350:CDS 45.0%
! CAGTCTTCACTGGCTATCAA+AGG - chr8_1:5439454-5439473 Msa1174350:CDS 45.0%
TTGGTGAATTAGGTTCCCGA+AGG - chr8_1:5439595-5439614 Msa1174350:CDS 45.0%
AAGTCCCTCAAATGTCGTCA+AGG - chr8_1:5439657-5439676 Msa1174350:CDS 45.0%
GTAAGCCTTGACGACATTTG+AGG + chr8_1:5439665-5439684 None:intergenic 45.0%
!! TTTTCTCAAGTGTCGCGCAA+AGG - chr8_1:5439714-5439733 Msa1174350:CDS 45.0%
GCGCAAAGGAATTGGTTGAA+GGG - chr8_1:5439728-5439747 Msa1174350:CDS 45.0%
CGCAAAGGAATTGGTTGAAG+GGG - chr8_1:5439729-5439748 Msa1174350:CDS 45.0%
GCAAAGGAATTGGTTGAAGG+GGG - chr8_1:5439730-5439749 Msa1174350:CDS 45.0%
TCGGAGACGGATAATAGTGA+GGG + chr8_1:5439987-5440006 None:intergenic 45.0%
TTCGGAGACGGATAATAGTG+AGG + chr8_1:5439988-5440007 None:intergenic 45.0%
TTATCCGTCTCCGAATGAAG+TGG - chr8_1:5439993-5440012 Msa1174350:CDS 45.0%
AACTCCACTTCATTCGGAGA+CGG + chr8_1:5440000-5440019 None:intergenic 45.0%
TCTGCAATTGCAAGGCTTCT+GGG + chr8_1:5439143-5439162 None:intergenic 45.0%
ATCTGCAATTGCAAGGCTTC+TGG + chr8_1:5439144-5439163 None:intergenic 45.0%
AAGCCTTGCAATTGCAGATC+TGG - chr8_1:5439145-5439164 Msa1174350:exon 45.0%
AGCCTTGCAATTGCAGATCT+GGG - chr8_1:5439146-5439165 Msa1174350:exon 45.0%
AACCCAGATCTGCAATTGCA+AGG + chr8_1:5439151-5439170 None:intergenic 45.0%
! ATGGGTCCTTGCTACTTCTT+TGG - chr8_1:5439365-5439384 Msa1174350:CDS 45.0%
GGAACACCAAAGAAGTAGCA+AGG + chr8_1:5439374-5439393 None:intergenic 45.0%
!! TGCTACTTCTTTGGTGTTCC+CGG - chr8_1:5439374-5439393 Msa1174350:CDS 45.0%
ACGAAATGCAGACTTCGATC+TGG + chr8_1:5439422-5439441 None:intergenic 45.0%
AGTGAAGACTGTAAGAGCAG+TGG + chr8_1:5439445-5439464 None:intergenic 45.0%
ACTGCTCTTACAGTCTTCAC+TGG - chr8_1:5439444-5439463 Msa1174350:CDS 45.0%
! CAGTCTTCACTGGCTATCAA+AGG - chr8_1:5439454-5439473 Msa1174350:CDS 45.0%
TTGGTGAATTAGGTTCCCGA+AGG - chr8_1:5439595-5439614 Msa1174350:CDS 45.0%
AAGTCCCTCAAATGTCGTCA+AGG - chr8_1:5439657-5439676 Msa1174350:CDS 45.0%
GTAAGCCTTGACGACATTTG+AGG + chr8_1:5439665-5439684 None:intergenic 45.0%
!! TTTTCTCAAGTGTCGCGCAA+AGG - chr8_1:5439714-5439733 Msa1174350:CDS 45.0%
GCGCAAAGGAATTGGTTGAA+GGG - chr8_1:5439728-5439747 Msa1174350:CDS 45.0%
CGCAAAGGAATTGGTTGAAG+GGG - chr8_1:5439729-5439748 Msa1174350:CDS 45.0%
GCAAAGGAATTGGTTGAAGG+GGG - chr8_1:5439730-5439749 Msa1174350:CDS 45.0%
TCGGAGACGGATAATAGTGA+GGG + chr8_1:5439987-5440006 None:intergenic 45.0%
TTCGGAGACGGATAATAGTG+AGG + chr8_1:5439988-5440007 None:intergenic 45.0%
TTATCCGTCTCCGAATGAAG+TGG - chr8_1:5439993-5440012 Msa1174350:CDS 45.0%
AACTCCACTTCATTCGGAGA+CGG + chr8_1:5440000-5440019 None:intergenic 45.0%
! GCTGGAGTAGAGGCTAGTTA+TGG + chr8_1:5439114-5439133 None:intergenic 50.0%
! TGGGAAGTATGCTGGAGTAG+AGG + chr8_1:5439124-5439143 None:intergenic 50.0%
! AAGGCTTCTGGGAAGTATGC+TGG + chr8_1:5439132-5439151 None:intergenic 50.0%
GCAGATCTGGGTTGTTCTTG+TGG - chr8_1:5439158-5439177 Msa1174350:exon 50.0%
! CAAGTGTCGCGCAAAGGAAT+TGG - chr8_1:5439720-5439739 Msa1174350:CDS 50.0%
CGCGCAAAGGAATTGGTTGA+AGG - chr8_1:5439727-5439746 Msa1174350:CDS 50.0%
!! TCGCATGGTTCTAACGATGG+TGG - chr8_1:5439753-5439772 Msa1174350:CDS 50.0%
!! CGCATGGTTCTAACGATGGT+GGG - chr8_1:5439754-5439773 Msa1174350:CDS 50.0%
GTCTCCGAATGAAGTGGAGT+TGG - chr8_1:5439999-5440018 Msa1174350:CDS 50.0%
! GCTGGAGTAGAGGCTAGTTA+TGG + chr8_1:5439114-5439133 None:intergenic 50.0%
! TGGGAAGTATGCTGGAGTAG+AGG + chr8_1:5439124-5439143 None:intergenic 50.0%
! AAGGCTTCTGGGAAGTATGC+TGG + chr8_1:5439132-5439151 None:intergenic 50.0%
GCAGATCTGGGTTGTTCTTG+TGG - chr8_1:5439158-5439177 Msa1174350:exon 50.0%
! CAAGTGTCGCGCAAAGGAAT+TGG - chr8_1:5439720-5439739 Msa1174350:CDS 50.0%
CGCGCAAAGGAATTGGTTGA+AGG - chr8_1:5439727-5439746 Msa1174350:CDS 50.0%
!! TCGCATGGTTCTAACGATGG+TGG - chr8_1:5439753-5439772 Msa1174350:CDS 50.0%
!! CGCATGGTTCTAACGATGGT+GGG - chr8_1:5439754-5439773 Msa1174350:CDS 50.0%
GTCTCCGAATGAAGTGGAGT+TGG - chr8_1:5439999-5440018 Msa1174350:CDS 50.0%
ATTGGTTGAAGGGGGTCGCA+TGG - chr8_1:5439738-5439757 Msa1174350:CDS 55.0%
!! GGGTCGCATGGTTCTAACGA+TGG - chr8_1:5439750-5439769 Msa1174350:CDS 55.0%
TGCAGGGTGCATTAGAGCTG+TGG - chr8_1:5440125-5440144 Msa1174350:CDS 55.0%
ATTGGTTGAAGGGGGTCGCA+TGG - chr8_1:5439738-5439757 Msa1174350:CDS 55.0%
!! GGGTCGCATGGTTCTAACGA+TGG - chr8_1:5439750-5439769 Msa1174350:CDS 55.0%
TGCAGGGTGCATTAGAGCTG+TGG - chr8_1:5440125-5440144 Msa1174350:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 5438947 5440503 5438947 ID=Msa1174350;Name=Msa1174350
chr8_1 mRNA 5438947 5440503 5438947 ID=Msa1174350-mRNA-1;Parent=Msa1174350;Name=Msa1174350-mRNA-1;_AED=0.05;_eAED=0.05;_QI=268|1|1|1|1|1|3|213|287
chr8_1 exon 5438947 5439504 5438947 ID=Msa1174350-mRNA-1:exon:1819;Parent=Msa1174350-mRNA-1
chr8_1 exon 5439586 5439843 5439586 ID=Msa1174350-mRNA-1:exon:1818;Parent=Msa1174350-mRNA-1
chr8_1 exon 5439975 5440503 5439975 ID=Msa1174350-mRNA-1:exon:1817;Parent=Msa1174350-mRNA-1
chr8_1 five_prime_UTR 5440236 5440503 5440236 ID=Msa1174350-mRNA-1:five_prime_utr;Parent=Msa1174350-mRNA-1
chr8_1 CDS 5439975 5440235 5439975 ID=Msa1174350-mRNA-1:cds;Parent=Msa1174350-mRNA-1
chr8_1 CDS 5439586 5439843 5439586 ID=Msa1174350-mRNA-1:cds;Parent=Msa1174350-mRNA-1
chr8_1 CDS 5439160 5439504 5439160 ID=Msa1174350-mRNA-1:cds;Parent=Msa1174350-mRNA-1
chr8_1 three_prime_UTR 5438947 5439159 5438947 ID=Msa1174350-mRNA-1:three_prime_utr;Parent=Msa1174350-mRNA-1
Gene Sequence

>Msa1174350

ATGGTTGTGGAGAAGCTTTGCCAACAATTGAAATATGCATCTCCCGAATACAAGATCTTTTTTAATGATCTTTCGGGGAATGACTTCAACAACATTTTTAAGTCACTTGATAGCTTTAAACATAAACTACTTGATGAAATCAAAACTGAAATGGGTCCTTGCTACTTCTTTGGTGTTCCCGGTTCTTTCTATGATAGAGTTTTTCCAGATCGAAGTCTGCATTTCGTCCACTGCTCTTACAGTCTTCACTGGCTATCAAAGGTTCCCGAAGGTATAGATAACAATAAGGGCAACATTTACATCAGCAACACAAGTCCCTCAAATGTCGTCAAGGCTTACTACGAGCAATTTCAAAGAGACTTATCGATTTTTCTCAAGTGTCGCGCAAAGGAATTGGTTGAAGGGGGTCGCATGGTTCTAACGATGGTGGGAAGAAGAAATGAAGATCCATGTGACGTGGAATATTGTTGCGACGATTGGGATTTTTTGGCTACTGCTCTTAATGATATGGTGTTGCAGGGGATTATAAGGGAAGAACAAGTGAATACTTTTAACATCCCTCACTATTATCCGTCTCCGAATGAAGTGGAGTTGGAAGTTCTCAATGAAGGATCGTTTGCCATCAATCACATGGAGTTGTTTGAAACAGAATCAGATACTTCTAATGATGAAAGTGATTCTGAAAATGGATATGATGTTGCAGGGTGCATTAGAGCTGTGGTTGAACCTTTGCTAGTTAGTCACTTTGGTGAAGCTATCATTGAAGAGGTTTTTAGCCGTTACAAGGAATTATTGATTGATCAAATATCCAAGGAGAGAATGAATTCCGTTAATGTTACCATATCATTGACTAGAAAATCATAA
Protein sequence

>Msa1174350

MVVEKLCQQLKYASPEYKIFFNDLSGNDFNNIFKSLDSFKHKLLDEIKTEMGPCYFFGVPGSFYDRVFPDRSLHFVHCSYSLHWLSKVPEGIDNNKGNIYISNTSPSNVVKAYYEQFQRDLSIFLKCRAKELVEGGRMVLTMVGRRNEDPCDVEYCCDDWDFLATALNDMVLQGIIREEQVNTFNIPHYYPSPNEVELEVLNEGSFAINHMELFETESDTSNDESDSENGYDVAGCIRAVVEPLLVSHFGEAIIEEVFSRYKELLIDQISKERMNSVNVTISLTRKS*