AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1174920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174920 MtrunA17_Chr7g0250271 89.706 68 7 0 1 68 1 68 6.49e-38 124
Msa1174920 MtrunA17_Chr0c29g0494011 89.706 68 7 0 1 68 1 68 6.49e-38 124
Msa1174920 MtrunA17_Chr3g0143051 80.769 78 12 2 1 76 1 77 9.28e-35 117
Msa1174920 MtrunA17_Chr3g0120901 90.769 65 5 1 1 65 1 64 4.83e-34 119
Msa1174920 MtrunA17_Chr3g0142531 94.915 59 3 0 1 59 1 59 1.55e-33 112
Msa1174920 MtrunA17_Chr3g0142561 76.316 76 17 1 1 76 1 75 5.10e-32 109
Msa1174920 MtrunA17_Chr7g0236221 48.148 108 33 3 18 102 9 116 7.07e-24 89.4
Msa1174920 MtrunA17_Chr7g0226481 75.000 60 13 1 14 71 7 66 2.43e-22 89.7
Msa1174920 MtrunA17_Chr7g0227621 47.253 91 48 0 14 104 7 97 1.19e-21 82.8
Msa1174920 MtrunA17_Chr7g0227651 74.074 54 14 0 14 67 7 60 2.08e-21 87.8
Msa1174920 MtrunA17_Chr8g0378391 72.549 51 14 0 17 67 10 60 2.18e-21 81.3
Msa1174920 MtrunA17_Chr7g0227851 47.778 90 37 1 14 103 7 86 2.98e-20 84.3
Msa1174920 MtrunA17_Chr7g0274231 70.492 61 15 2 7 67 4 61 4.24e-20 82.8
Msa1174920 MtrunA17_Chr1g0166251 72.222 54 15 0 14 67 7 60 6.63e-20 83.6
Msa1174920 MtrunA17_Chr7g0227821 70.370 54 16 0 14 67 7 60 1.10e-19 81.3
Msa1174920 MtrunA17_Chr7g0262131 56.164 73 30 1 1 71 1 73 7.62e-19 76.6
Msa1174920 MtrunA17_Chr3g0136291 64.912 57 20 0 11 67 7 63 1.09e-18 79.7
Msa1174920 MtrunA17_Chr3g0124041 64.912 57 20 0 18 74 12 68 1.21e-18 75.1
Msa1174920 MtrunA17_Chr3g0091171 55.556 63 28 0 14 76 1 63 3.27e-18 78.6
Msa1174920 MtrunA17_Chr7g0227641 44.444 90 40 1 14 103 7 86 4.06e-18 78.6
Msa1174920 MtrunA17_Chr7g0262091 58.209 67 28 0 1 67 1 67 6.70e-18 78.2
Msa1174920 MtrunA17_Chr7g0236101 70.000 50 15 0 18 67 9 58 2.37e-17 73.6
Msa1174920 MtrunA17_Chr1g0167251 66.000 50 17 0 18 67 12 61 2.72e-17 70.9
Msa1174920 MtrunA17_Chr7g0262081 56.716 67 29 0 1 67 13 79 2.83e-17 76.3
Msa1174920 MtrunA17_Chr3g0093361 53.968 63 29 0 5 67 30 92 2.94e-17 76.3
Msa1174920 MtrunA17_Chr7g0236211 68.421 57 17 1 12 67 5 61 4.00e-17 75.9
Msa1174920 MtrunA17_Chr7g0236171 70.000 50 15 0 18 67 9 58 7.33e-17 72.4
Msa1174920 MtrunA17_Chr7g0236131 70.000 50 15 0 18 67 9 58 7.33e-17 72.4
Msa1174920 MtrunA17_Chr7g0236081 70.000 50 15 0 18 67 9 58 7.33e-17 72.4
Msa1174920 MtrunA17_Chr3g0139601 56.716 67 29 0 8 74 3 69 8.39e-17 75.1
Msa1174920 MtrunA17_Chr3g0138371 47.727 88 35 2 15 91 17 104 1.03e-16 74.7
Msa1174920 MtrunA17_Chr7g0236151 70.000 50 15 0 18 67 9 58 1.18e-16 73.9
Msa1174920 MtrunA17_Chr7g0236191 70.000 50 15 0 18 67 9 58 1.18e-16 73.9
Msa1174920 MtrunA17_Chr7g0238361 67.308 52 17 0 18 69 3 54 1.30e-16 74.3
Msa1174920 MtrunA17_Chr5g0396411 57.812 64 22 1 20 78 1 64 1.92e-16 73.9
Msa1174920 MtrunA17_Chr3g0138571 66.071 56 17 1 12 67 9 62 1.94e-16 73.9
Msa1174920 MtrunA17_Chr3g0086041 59.259 54 22 0 14 67 22 75 2.91e-16 73.6
Msa1174920 MtrunA17_Chr7g0273871 56.250 64 27 1 10 73 5 67 3.13e-16 73.2
Msa1174920 MtrunA17_Chr3g0086141 72.340 47 13 0 17 63 2 48 3.84e-16 71.2
Msa1174920 MtrunA17_Chr4g0076481 60.938 64 19 2 4 67 16 73 5.50e-16 71.2
Msa1174920 MtrunA17_Chr3g0137721 54.688 64 28 1 18 80 11 74 6.16e-16 72.4
Msa1174920 MtrunA17_Chr3g0107721 68.000 50 16 0 18 67 3 52 9.91e-16 72.0
Msa1174920 MtrunA17_Chr7g0262121 58.333 60 22 1 3 62 8 64 2.78e-15 70.9
Msa1174920 MtrunA17_Chr5g0438051 67.308 52 16 1 14 64 1 52 5.16e-15 70.1
Msa1174920 MtrunA17_Chr5g0396401 62.500 48 18 0 20 67 10 57 5.39e-15 69.7
Msa1174920 MtrunA17_Chr7g0227771 64.286 56 18 1 18 71 4 59 1.04e-14 67.4
Msa1174920 MtrunA17_Chr2g0278171 49.206 63 32 0 7 69 3 65 2.62e-14 65.1
Msa1174920 MtrunA17_Chr1g0198241 60.714 56 18 1 15 66 3 58 7.61e-14 62.8
Msa1174920 MtrunA17_Chr7g0215531 65.306 49 17 0 15 63 10 58 1.20e-13 63.9
Msa1174920 MtrunA17_Chr6g0467501 47.945 73 31 2 2 67 188 260 1.29e-13 66.2
Msa1174920 MtrunA17_Chr2g0319421 50.000 72 33 1 3 74 15 83 1.34e-13 62.8
Msa1174920 MtrunA17_Chr3g0137681 69.767 43 13 0 18 60 10 52 2.18e-13 65.5
Msa1174920 MtrunA17_Chr5g0437351 60.465 43 17 0 20 62 17 59 2.40e-13 61.6
Msa1174920 MtrunA17_Chr2g0278131 62.500 48 18 0 17 64 13 60 2.95e-13 60.8
Msa1174920 MtrunA17_Chr4g0011351 33.333 117 60 2 2 118 4 102 3.25e-13 63.5
Msa1174920 MtrunA17_Chr7g0255101 58.000 50 21 0 18 67 110 159 3.29e-13 65.1
Msa1174920 MtrunA17_Chr2g0277281 63.830 47 17 0 18 64 11 57 5.91e-13 63.9
Msa1174920 MtrunA17_Chr8g0358761 48.148 54 28 0 18 71 13 66 6.67e-13 59.7
Msa1174920 MtrunA17_Chr8g0363821 42.683 82 36 3 2 72 5 86 8.47e-13 63.5
Msa1174920 MtrunA17_Chr2g0277911 70.270 37 11 0 18 54 2 38 1.16e-12 58.9
Msa1174920 MtrunA17_Chr8g0363801 33.333 111 58 4 18 127 9 104 1.20e-12 60.8
Msa1174920 MtrunA17_Chr6g0466471 49.153 59 29 1 18 75 8 66 1.31e-12 59.3
Msa1174920 MtrunA17_Chr6g0466481 58.333 48 20 0 18 65 8 55 1.39e-12 63.2
Msa1174920 MtrunA17_Chr5g0425831 55.319 47 21 0 18 64 27 73 1.40e-12 58.9
Msa1174920 MtrunA17_Chr6g0465671 58.333 48 20 0 18 65 8 55 1.43e-12 63.2
Msa1174920 MtrunA17_Chr2g0278091 40.230 87 47 2 18 104 12 93 1.46e-12 62.8
Msa1174920 MtrunA17_Chr4g0014371 43.750 64 34 2 6 67 3 66 1.87e-12 60.5
Msa1174920 MtrunA17_Chr4g0014451 55.769 52 23 0 18 69 3 54 1.88e-12 62.4
Msa1174920 MtrunA17_Chr7g0215541 60.000 50 20 0 15 64 14 63 1.95e-12 62.4
Msa1174920 MtrunA17_Chr7g0246351 63.830 47 17 0 18 64 6 52 2.16e-12 62.4
Msa1174920 MtrunA17_Chr8g0340521 54.902 51 22 1 18 67 41 91 2.45e-12 60.8
Msa1174920 MtrunA17_Chr3g0132631 60.417 48 19 0 17 64 11 58 2.90e-12 62.0
Msa1174920 MtrunA17_Chr8g0340581 46.377 69 35 1 1 69 1 67 3.01e-12 60.5
Msa1174920 MtrunA17_Chr6g0465661 49.153 59 29 1 18 75 32 90 3.15e-12 58.9
Msa1174920 MtrunA17_Chr3g0129741 46.875 64 30 2 7 66 4 67 3.28e-12 60.1
Msa1174920 MtrunA17_Chr3g0137701 61.702 47 18 0 18 64 9 55 3.41e-12 62.0
Msa1174920 MtrunA17_Chr7g0235851 51.923 52 25 0 18 69 5 56 4.17e-12 61.6
Msa1174920 MtrunA17_Chr5g0405101 45.205 73 38 1 4 74 5 77 4.36e-12 60.1
Msa1174920 MtrunA17_Chr3g0085961 50.000 68 34 0 5 72 2 69 4.94e-12 61.6
Msa1174920 MtrunA17_Chr7g0235821 51.923 52 25 0 18 69 5 56 5.27e-12 61.2
Msa1174920 MtrunA17_Chr3g0129711 56.000 50 20 1 18 65 4 53 5.37e-12 58.2
Msa1174920 MtrunA17_Chr2g0319401 50.000 64 26 1 1 64 16 73 7.15e-12 60.8
Msa1174920 MtrunA17_Chr2g0278801 56.140 57 25 0 18 74 2 58 7.37e-12 60.5
Msa1174920 MtrunA17_Chr5g0431711 55.102 49 22 0 18 66 11 59 8.36e-12 60.8
Msa1174920 MtrunA17_Chr2g0332991 57.143 49 21 0 13 61 4 52 9.59e-12 58.2
Msa1174920 MtrunA17_Chr2g0278121 57.143 49 21 0 16 64 14 62 1.05e-11 60.5
Msa1174920 MtrunA17_Chr8g0369261 56.364 55 20 2 1 54 1 52 1.06e-11 60.5
Msa1174920 MtrunA17_Chr6g0466531 50.000 56 28 0 20 75 10 65 1.19e-11 57.4
Msa1174920 MtrunA17_Chr7g0224001 59.574 47 19 0 18 64 14 60 1.28e-11 60.5
Msa1174920 MtrunA17_Chr4g0049751 45.161 62 34 0 1 62 1 62 1.49e-11 60.1
Msa1174920 MtrunA17_Chr8g0363941 39.706 68 32 1 15 82 13 71 1.61e-11 58.5
Msa1174920 MtrunA17_Chr4g0049041 49.231 65 28 1 15 74 22 86 1.69e-11 60.1
Msa1174920 MtrunA17_Chr7g0227221 55.769 52 21 1 17 66 13 64 2.16e-11 56.2
Msa1174920 MtrunA17_Chr7g0242471 40.000 60 36 0 14 73 1 60 2.49e-11 57.8
Msa1174920 MtrunA17_Chr6g0453781 59.091 44 18 0 18 61 9 52 2.51e-11 59.3
Msa1174920 MtrunA17_Chr3g0091151 68.421 38 12 0 14 51 3 40 2.81e-11 55.5
Msa1174920 MtrunA17_Chr5g0423901 57.447 47 20 0 18 64 9 55 2.93e-11 59.3
Msa1174920 MtrunA17_Chr2g0278791 54.386 57 26 0 18 74 2 58 3.02e-11 59.3
Msa1174920 MtrunA17_Chr2g0278151 54.167 48 22 0 17 64 18 65 3.35e-11 58.9
Msa1174920 MtrunA17_Chr8g0362521 40.000 65 39 0 7 71 4 68 3.47e-11 58.9
Msa1174920 MtrunA17_Chr4g0041681 55.556 45 20 0 18 62 17 61 3.95e-11 58.9
Msa1174920 MtrunA17_Chr6g0465731 50.000 56 28 0 20 75 10 65 4.10e-11 58.9
Msa1174920 MtrunA17_Chr5g0435741 52.000 50 24 0 16 65 31 80 4.48e-11 58.9
Msa1174920 MtrunA17_Chr2g0319411 52.632 57 27 0 18 74 22 78 4.76e-11 58.5
Msa1174920 MtrunA17_Chr8g0340611 52.083 48 23 0 16 63 8 55 4.99e-11 57.0
Msa1174920 MtrunA17_Chr7g0222301 48.000 50 26 0 4 53 35 84 5.04e-11 58.5
Msa1174920 MtrunA17_Chr1g0208801 55.102 49 22 0 17 65 7 55 5.36e-11 58.5
Msa1174920 MtrunA17_Chr1g0199541 42.391 92 43 2 1 82 9 100 5.46e-11 58.2
Msa1174920 MtrunA17_Chr5g0431681 59.574 47 19 0 18 64 8 54 5.49e-11 58.5
Msa1174920 MtrunA17_Chr3g0087391 55.319 47 21 0 18 64 19 65 5.62e-11 58.5
Msa1174920 MtrunA17_Chr7g0222241 38.462 65 40 0 4 68 35 99 6.03e-11 58.5
Msa1174920 MtrunA17_Chr7g0222281 38.462 65 40 0 4 68 35 99 6.03e-11 58.5
Msa1174920 MtrunA17_Chr8g0360071 61.702 47 15 2 18 61 13 59 6.11e-11 58.5
Msa1174920 MtrunA17_Chr8g0362021 52.083 48 23 0 18 65 20 67 6.40e-11 58.5
Msa1174920 MtrunA17_Chr5g0395141 65.116 43 15 0 23 65 20 62 8.24e-11 55.8
Msa1174920 MtrunA17_Chr5g0425131 39.241 79 40 1 18 96 11 81 9.12e-11 57.4
Msa1174920 MtrunA17_Chr3g0085901 56.000 50 22 0 18 67 12 61 9.62e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174920 AT5G41840.1 47.826 69 36 0 6 74 2 70 2.47e-15 71.2
Msa1174920 AT1G32020.1 35.106 94 57 2 16 105 2 95 3.21e-14 65.9
Msa1174920 AT5G41830.2 53.846 52 24 0 18 69 13 64 2.94e-13 65.5
Msa1174920 AT1G16930.2 48.000 75 28 2 5 74 8 76 3.87e-13 65.1
Msa1174920 AT1G16930.1 48.000 75 28 2 5 74 8 76 3.87e-13 65.1
Msa1174920 AT1G16930.3 48.000 75 28 2 5 74 8 76 3.87e-13 65.1
Msa1174920 AT3G59200.2 54.000 50 23 0 18 67 7 56 9.87e-13 63.9
Msa1174920 AT3G59200.1 54.000 50 23 0 18 67 7 56 1.32e-12 63.5
Msa1174920 AT5G27750.1 60.000 50 20 0 18 67 5 54 1.43e-12 63.5
Msa1174920 AT2G26030.3 54.902 51 23 0 17 67 3 53 2.17e-12 62.8
Msa1174920 AT2G26030.1 54.902 51 23 0 17 67 3 53 2.17e-12 62.8
Msa1174920 AT2G26030.4 54.902 51 23 0 17 67 3 53 2.17e-12 62.8
Msa1174920 AT3G12840.1 47.368 57 30 0 19 75 16 72 3.08e-12 60.8
Msa1174920 AT5G18780.2 58.000 50 21 0 18 67 11 60 3.32e-12 62.4
Msa1174920 AT5G18780.1 58.000 50 21 0 18 67 11 60 3.32e-12 62.4
Msa1174920 AT5G18780.3 58.000 50 21 0 18 67 11 60 3.45e-12 62.4
Msa1174920 AT5G18780.4 58.000 50 21 0 18 67 11 60 3.45e-12 62.4
Msa1174920 AT4G00320.1 57.447 47 20 0 18 64 13 59 3.47e-12 62.4
Msa1174920 AT5G41630.1 61.702 47 18 0 18 64 12 58 3.64e-12 62.4
Msa1174920 AT5G53640.1 48.387 62 30 1 18 77 22 83 3.72e-12 61.2
Msa1174920 AT4G00160.1 54.000 50 23 0 18 67 16 65 4.55e-12 62.0
Msa1174920 AT1G19070.1 47.541 61 29 1 18 78 2 59 4.75e-12 58.2
Msa1174920 AT3G59150.1 32.168 143 75 4 1 127 1 137 6.64e-12 61.6
Msa1174920 AT5G27750.2 60.000 50 20 0 18 67 5 54 9.91e-12 60.8
Msa1174920 AT1G58310.1 57.447 47 20 0 18 64 8 54 1.59e-11 60.5
Msa1174920 AT1G78760.1 38.889 90 41 3 2 82 5 89 1.93e-11 60.1
Msa1174920 AT5G22610.1 54.000 50 23 0 18 67 18 67 2.20e-11 60.1
Msa1174920 AT5G22610.2 54.000 50 23 0 18 67 18 67 2.34e-11 60.1
Msa1174920 AT4G26340.1 47.619 63 27 1 18 74 2 64 2.99e-11 59.7
Msa1174920 AT5G22700.2 66.667 39 13 0 18 56 6 44 3.26e-11 59.7
Msa1174920 AT5G22700.3 66.667 39 13 0 18 56 6 44 3.26e-11 59.7
Msa1174920 AT5G22700.1 66.667 39 13 0 18 56 6 44 3.26e-11 59.7
Msa1174920 AT5G22700.4 66.667 39 13 0 18 56 6 44 3.26e-11 59.7
Msa1174920 AT5G22700.5 66.667 39 13 0 18 56 43 81 3.28e-11 59.7
Msa1174920 AT5G44960.1 51.852 54 26 0 14 67 1 54 3.38e-11 59.7
Msa1174920 AT5G22700.6 66.667 39 13 0 18 56 43 81 3.38e-11 59.7
Msa1174920 AT3G58940.1 54.902 51 23 0 18 68 2 52 3.69e-11 59.3
Msa1174920 AT3G58940.2 54.902 51 23 0 18 68 2 52 3.69e-11 59.3
Msa1174920 AT5G22730.2 56.000 50 22 0 18 67 28 77 6.18e-11 58.9
Msa1174920 AT1G69630.1 49.180 61 29 1 15 73 15 75 6.22e-11 58.9
Msa1174920 AT3G58930.4 54.902 51 23 0 18 68 2 52 6.39e-11 58.9
Msa1174920 AT3G58930.2 54.902 51 23 0 18 68 2 52 6.39e-11 58.9
Msa1174920 AT3G58930.3 54.902 51 23 0 18 68 2 52 6.67e-11 58.5
Msa1174920 AT3G58930.1 54.902 51 23 0 18 68 2 52 6.67e-11 58.5
Msa1174920 AT3G58930.5 54.902 51 23 0 18 68 2 52 6.67e-11 58.5
Msa1174920 AT3G03040.1 54.902 51 23 0 18 68 2 52 6.84e-11 58.5
Msa1174920 AT5G22730.1 56.000 50 22 0 18 67 28 77 6.89e-11 58.5
Msa1174920 AT5G22730.3 56.000 50 22 0 18 67 28 77 6.89e-11 58.5
Msa1174920 AT5G53840.1 55.000 60 25 1 16 73 16 75 7.52e-11 58.5
Msa1174920 AT3G03030.2 54.902 51 23 0 18 68 2 52 7.57e-11 58.5
Msa1174920 AT3G03030.1 54.902 51 23 0 18 68 2 52 7.99e-11 58.5
Msa1174920 AT5G22720.1 48.387 62 27 1 18 74 23 84 8.29e-11 58.5
Msa1174920 AT4G10400.2 52.000 50 24 0 18 67 2 51 8.45e-11 58.5
Msa1174920 AT4G10400.3 52.000 50 24 0 18 67 2 51 8.45e-11 58.5
Msa1174920 AT4G10400.1 52.000 50 24 0 18 67 2 51 8.45e-11 58.5
Msa1174920 AT5G56820.1 37.931 87 48 1 18 104 15 95 8.58e-11 58.5
Msa1174920 AT3G58860.1 50.000 54 27 0 15 68 4 57 8.83e-11 58.5

Find 83 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCAAATGCTTGCCCAATT+TGG 0.243250 8_1:-6152178 Msa1174920:three_prime_UTR
ATAATATTATACTCACTCTT+TGG 0.276797 8_1:+6152851 None:intergenic
AAGCTTGGCTGTCACCAAAT+TGG 0.276858 8_1:+6152164 None:intergenic
TAATATTATACTCACTCTTT+GGG 0.278133 8_1:+6152852 None:intergenic
CTTCCAACATATTCACTTGT+AGG 0.295079 8_1:-6152430 Msa1174920:exon
CAAAGAGATGGAAACCATTA+TGG 0.303244 8_1:-6152674 Msa1174920:CDS
AGCGCTGTTGGCTTTAAATA+AGG 0.309434 8_1:+6153063 None:intergenic
GCTTGTGGCGACGGTGTCTT+TGG 0.336244 8_1:+6152704 None:intergenic
GGCTGGCTGGCTAGCGCTGT+TGG 0.340825 8_1:+6153051 None:intergenic
GAAAGCAGGAAGAGAGGAAT+AGG 0.350371 8_1:+6152129 None:intergenic
AGCATCCACATCCACTTCTT+AGG 0.366035 8_1:+6151907 None:intergenic
AGCTTGGCTGTCACCAAATT+GGG 0.366685 8_1:+6152165 None:intergenic
TGTTTGCAATTCCTCTAGAA+TGG 0.403902 8_1:+6152254 None:intergenic
ATGATAAGATGTTGAAGCTT+TGG 0.408194 8_1:+6151865 None:intergenic
GGTGTCTTTGGTTGGGAGAA+AGG 0.415987 8_1:+6152716 None:intergenic
GAGCTATTCTCTAATGTAAA+GGG 0.420946 8_1:-6151940 Msa1174920:three_prime_UTR
AATATTTAATAAACAACTGC+TGG 0.423210 8_1:+6151204 None:intergenic
ACCCTGTCGCACTCTTCAAT+TGG 0.424294 8_1:+6152777 None:intergenic
CGACAGGGTTAGCTCCCTTC+CGG 0.429890 8_1:-6152763 Msa1174920:CDS
CAAAGAAGCAGCAGCGATCA+TGG 0.433969 8_1:-6152803 Msa1174920:CDS
TCGCTGCTGCTTCTTTGACT+TGG 0.438632 8_1:+6152809 None:intergenic
TTAATTTCAACACTCATAAA+CGG 0.440294 8_1:-6152302 Msa1174920:three_prime_UTR
TCCTCTCTTCCTGCTTTCCA+AGG 0.442960 8_1:-6152124 Msa1174920:three_prime_UTR
GTGAGGACTGAGAGCCATAA+TGG 0.445802 8_1:+6152660 None:intergenic
GTTTGCAATTCCTCTAGAAT+GGG 0.446597 8_1:+6152255 None:intergenic
GTGGCGACGGTGTCTTTGGT+TGG 0.449866 8_1:+6152708 None:intergenic
AATTTCACATGTCAAGTTCA+AGG 0.456173 8_1:+6152455 None:intergenic
TGGCGACGGTGTCTTTGGTT+GGG 0.461534 8_1:+6152709 None:intergenic
TGAGTAGCGGATTCAATGGC+TGG 0.463741 8_1:+6153034 None:intergenic
AATAAGTTGAGTGATATCAT+TGG 0.463912 8_1:+6152507 None:intergenic
ATAAGTTGAGTGATATCATT+GGG 0.464892 8_1:+6152508 None:intergenic
TTCCAATTGAAGAGTGCGAC+AGG 0.466848 8_1:-6152779 Msa1174920:CDS
GGAGCTATTCTCTAATGTAA+AGG 0.470359 8_1:-6151941 Msa1174920:three_prime_UTR
ACTCAAAAGGAGATGAGATT+AGG 0.473899 8_1:-6153016 Msa1174920:five_prime_UTR
GAGTCCGTGCCTTGGAAAGC+AGG 0.474538 8_1:+6152115 None:intergenic
CATTGAATCCGCTACTCAAA+AGG 0.477575 8_1:-6153029 Msa1174920:five_prime_UTR
ATGCAGACATGGACCCACTA+TGG 0.492772 8_1:-6151997 Msa1174920:intron
ATAGCATGACTGAGTACATC+TGG 0.496230 8_1:+6152596 None:intergenic
TAGCGGATTCAATGGCTGGC+TGG 0.497466 8_1:+6153038 None:intergenic
AGGTTTGTTATCAGAAAGCT+TGG 0.498630 8_1:+6152149 None:intergenic
CTTGTCCTTTCCCATTCTAG+AGG 0.504356 8_1:-6152265 Msa1174920:three_prime_UTR
GCATAGCTACATGGATCAAA+GGG 0.521045 8_1:-6151173 Msa1174920:three_prime_UTR
AAATAGGGACTTGACCATAG+TGG 0.525483 8_1:+6151983 None:intergenic
AATATTATACTCACTCTTTG+GGG 0.525869 8_1:+6152853 None:intergenic
TTGAATCTCGTGGCGTGGCG+TGG 0.530443 8_1:+6152892 None:intergenic
TGAGAGAATGCTTGTGGCGA+CGG 0.533104 8_1:+6152695 None:intergenic
TCTTCCTGCTTTCCAAGGCA+CGG 0.537877 8_1:-6152119 Msa1174920:three_prime_UTR
ATTTCACATGTCAAGTTCAA+GGG 0.540030 8_1:+6152456 None:intergenic
CAAGCATTCTCTCAAAGAGA+TGG 0.543124 8_1:-6152686 Msa1174920:CDS
TGCATAGCTACATGGATCAA+AGG 0.543192 8_1:-6151174 Msa1174920:three_prime_UTR
ATATGACATATAATTGAGTC+CGG 0.544933 8_1:+6152744 None:intergenic
GGTTGAGCTAGAGGTGTGAA+TGG 0.545726 8_1:+6152950 None:intergenic
CTAATGTAAAGGGTAAAAGT+TGG 0.545813 8_1:-6151930 Msa1174920:three_prime_UTR
GACATATAATTGAGTCCGGA+AGG 0.551664 8_1:+6152748 None:intergenic
TATACTGAGAGTCCGTGCCT+TGG 0.551730 8_1:+6152107 None:intergenic
CAATTCCTCTAGAATGGGAA+AGG 0.553185 8_1:+6152260 None:intergenic
AATAGGGACTTGACCATAGT+GGG 0.554866 8_1:+6151984 None:intergenic
TGAGATGATGCATAGCTACA+TGG 0.555182 8_1:-6151182 Msa1174920:three_prime_UTR
ACTACTCAAAACTCTCCACT+TGG 0.555992 8_1:-6152333 Msa1174920:three_prime_UTR
TCTCGTGGCGTGGCGTGGCG+TGG 0.566244 8_1:+6152897 None:intergenic
TCAACATCTTATCATTCATG+AGG 0.570247 8_1:-6151857 Msa1174920:intron
TTGAGGCGGAGTGATAGTAT+TGG 0.571221 8_1:+6152556 None:intergenic
GCCTTGGAAAGCAGGAAGAG+AGG 0.573004 8_1:+6152123 None:intergenic
ATTGCAAACAAATTACGTAA+AGG 0.574530 8_1:-6152241 Msa1174920:three_prime_UTR
ACGGACTCTCAGTATAAAGC+TGG 0.574567 8_1:-6152100 Msa1174920:intron
TGTCCTACAAGTGAATATGT+TGG 0.588152 8_1:+6152427 None:intergenic
GTAAAAGTTGGCCTAAGAAG+TGG 0.590931 8_1:-6151918 Msa1174920:three_prime_UTR
GTGTGAATGGTGTTTCAGAT+CGG 0.595902 8_1:+6152963 None:intergenic
TCTCTTTGAGAGAATGCTTG+TGG 0.597896 8_1:+6152689 None:intergenic
GAACGGTGTGGTTGAGCTAG+AGG 0.600009 8_1:+6152941 None:intergenic
CGATTAATTGTTGAACGGTG+TGG 0.607379 8_1:+6152929 None:intergenic
ATATTATACTCACTCTTTGG+GGG 0.612790 8_1:+6152854 None:intergenic
TAGTGAGAAGAATTCGTGAT+CGG 0.621496 8_1:+6152626 None:intergenic
GTTGGCCTAAGAAGTGGATG+TGG 0.622046 8_1:-6151912 Msa1174920:three_prime_UTR
ACATATAATTGAGTCCGGAA+GGG 0.623798 8_1:+6152749 None:intergenic
TTATTTGAGGAATGTCTCTG+AGG 0.633241 8_1:+6152372 None:intergenic
GATCGGTGAAATCGAGAGTG+AGG 0.633584 8_1:+6152643 None:intergenic
TCCAATTGAAGAGTGCGACA+GGG 0.638921 8_1:-6152778 Msa1174920:CDS
TGAATCGATTAATTGTTGAA+CGG 0.641285 8_1:+6152924 None:intergenic
CGAAGTTGAATCTCGTGGCG+TGG 0.647210 8_1:+6152887 None:intergenic
CACGCCACGAGATTCAACTT+CGG 0.658198 8_1:-6152886 Msa1174920:five_prime_UTR
TAATCCGAAGTTGAATCTCG+TGG 0.659751 8_1:+6152882 None:intergenic
AAATTAACACAAAATCCAAG+TGG 0.668914 8_1:+6152318 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACATTTTCAATTATGTAT+TGG - chr8_1:6152905-6152924 Msa1174920:five_prime_UTR 15.0%
!!! TTACATTTTCAATTATGTAT+TGG - chr8_1:6152905-6152924 Msa1174920:five_prime_UTR 15.0%
!! TAATATTATACTCACTCTTT+GGG + chr8_1:6151388-6151407 None:intergenic 20.0%
!! ATAATATTATACTCACTCTT+TGG + chr8_1:6151389-6151408 None:intergenic 20.0%
!! TTAATTTCAACACTCATAAA+CGG - chr8_1:6151935-6151954 Msa1174920:three_prime_UTR 20.0%
!! GAGTTAGATTGTAAAAAATA+GGG + chr8_1:6152272-6152291 None:intergenic 20.0%
!! TGAGTTAGATTGTAAAAAAT+AGG + chr8_1:6152273-6152292 None:intergenic 20.0%
!!! TTTTTACAATCTAACTCAAA+TGG - chr8_1:6152275-6152294 Msa1174920:three_prime_UTR 20.0%
!!! TTAACTTTTGTTTCTTGTTT+TGG - chr8_1:6152459-6152478 Msa1174920:CDS 20.0%
!!! TAACTTTTGTTTCTTGTTTT+GGG - chr8_1:6152460-6152479 Msa1174920:CDS 20.0%
!!! GAATTTTACTTATTGTTTAC+AGG - chr8_1:6152485-6152504 Msa1174920:CDS 20.0%
!!! GTATTTTGCATTTAAATCTA+TGG + chr8_1:6152712-6152731 None:intergenic 20.0%
!! TAGATTTAAATGCAAAATAC+CGG - chr8_1:6152712-6152731 Msa1174920:CDS 20.0%
!! AAACTTATATTTGAATGACT+AGG - chr8_1:6152771-6152790 Msa1174920:CDS 20.0%
!! AACTTATATTTGAATGACTA+GGG - chr8_1:6152772-6152791 Msa1174920:CDS 20.0%
!! AATATTTAATAAACAACTGC+TGG + chr8_1:6153036-6153055 None:intergenic 20.0%
!! TAATATTATACTCACTCTTT+GGG + chr8_1:6151388-6151407 None:intergenic 20.0%
!! ATAATATTATACTCACTCTT+TGG + chr8_1:6151389-6151408 None:intergenic 20.0%
!! TTAATTTCAACACTCATAAA+CGG - chr8_1:6151935-6151954 Msa1174920:three_prime_UTR 20.0%
!! GAGTTAGATTGTAAAAAATA+GGG + chr8_1:6152272-6152291 None:intergenic 20.0%
!! TGAGTTAGATTGTAAAAAAT+AGG + chr8_1:6152273-6152292 None:intergenic 20.0%
!!! TTTTTACAATCTAACTCAAA+TGG - chr8_1:6152275-6152294 Msa1174920:three_prime_UTR 20.0%
!!! TTAACTTTTGTTTCTTGTTT+TGG - chr8_1:6152459-6152478 Msa1174920:CDS 20.0%
!!! TAACTTTTGTTTCTTGTTTT+GGG - chr8_1:6152460-6152479 Msa1174920:CDS 20.0%
!!! GAATTTTACTTATTGTTTAC+AGG - chr8_1:6152485-6152504 Msa1174920:CDS 20.0%
!!! GTATTTTGCATTTAAATCTA+TGG + chr8_1:6152712-6152731 None:intergenic 20.0%
!! TAGATTTAAATGCAAAATAC+CGG - chr8_1:6152712-6152731 Msa1174920:CDS 20.0%
!! AAACTTATATTTGAATGACT+AGG - chr8_1:6152771-6152790 Msa1174920:CDS 20.0%
!! AACTTATATTTGAATGACTA+GGG - chr8_1:6152772-6152791 Msa1174920:CDS 20.0%
!! AATATTTAATAAACAACTGC+TGG + chr8_1:6153036-6153055 None:intergenic 20.0%
! TGAATCGATTAATTGTTGAA+CGG + chr8_1:6151316-6151335 None:intergenic 25.0%
! AATATTATACTCACTCTTTG+GGG + chr8_1:6151387-6151406 None:intergenic 25.0%
! ATATGACATATAATTGAGTC+CGG + chr8_1:6151496-6151515 None:intergenic 25.0%
!! CAACTATATAACAACTTTTG+AGG + chr8_1:6151701-6151720 None:intergenic 25.0%
! CAAAAGTTGTTATATAGTTG+AGG - chr8_1:6151701-6151720 Msa1174920:intron 25.0%
!! ATAAGTTGAGTGATATCATT+GGG + chr8_1:6151732-6151751 None:intergenic 25.0%
!! AATAAGTTGAGTGATATCAT+TGG + chr8_1:6151733-6151752 None:intergenic 25.0%
! GTAGTAGTATTACTTATTTG+AGG + chr8_1:6151881-6151900 None:intergenic 25.0%
! AAATTAACACAAAATCCAAG+TGG + chr8_1:6151922-6151941 None:intergenic 25.0%
! ATTGCAAACAAATTACGTAA+AGG - chr8_1:6151996-6152015 Msa1174920:intron 25.0%
! ACAAAAGTTAAGATATGTAG+AGG + chr8_1:6152450-6152469 None:intergenic 25.0%
! ATTGAAAATAGGATAGAGAA+TGG - chr8_1:6152510-6152529 Msa1174920:CDS 25.0%
! AAATGATGTTAAGGACAATT+GGG - chr8_1:6152533-6152552 Msa1174920:intron 25.0%
! ACTTATATTTGAATGACTAG+GGG - chr8_1:6152773-6152792 Msa1174920:CDS 25.0%
! ACAATCAGTAAGAAAAAGAA+AGG + chr8_1:6152979-6152998 None:intergenic 25.0%
! TGAATCGATTAATTGTTGAA+CGG + chr8_1:6151316-6151335 None:intergenic 25.0%
! AATATTATACTCACTCTTTG+GGG + chr8_1:6151387-6151406 None:intergenic 25.0%
! ATATGACATATAATTGAGTC+CGG + chr8_1:6151496-6151515 None:intergenic 25.0%
!! CAACTATATAACAACTTTTG+AGG + chr8_1:6151701-6151720 None:intergenic 25.0%
! CAAAAGTTGTTATATAGTTG+AGG - chr8_1:6151701-6151720 Msa1174920:intron 25.0%
!! ATAAGTTGAGTGATATCATT+GGG + chr8_1:6151732-6151751 None:intergenic 25.0%
!! AATAAGTTGAGTGATATCAT+TGG + chr8_1:6151733-6151752 None:intergenic 25.0%
! GTAGTAGTATTACTTATTTG+AGG + chr8_1:6151881-6151900 None:intergenic 25.0%
! AAATTAACACAAAATCCAAG+TGG + chr8_1:6151922-6151941 None:intergenic 25.0%
! ATTGCAAACAAATTACGTAA+AGG - chr8_1:6151996-6152015 Msa1174920:intron 25.0%
! ACAAAAGTTAAGATATGTAG+AGG + chr8_1:6152450-6152469 None:intergenic 25.0%
! ATTGAAAATAGGATAGAGAA+TGG - chr8_1:6152510-6152529 Msa1174920:CDS 25.0%
! AAATGATGTTAAGGACAATT+GGG - chr8_1:6152533-6152552 Msa1174920:intron 25.0%
! ACTTATATTTGAATGACTAG+GGG - chr8_1:6152773-6152792 Msa1174920:CDS 25.0%
! ACAATCAGTAAGAAAAAGAA+AGG + chr8_1:6152979-6152998 None:intergenic 25.0%
ATATTATACTCACTCTTTGG+GGG + chr8_1:6151386-6151405 None:intergenic 30.0%
!! CTATATAACAACTTTTGAGG+CGG + chr8_1:6151698-6151717 None:intergenic 30.0%
AAGTTGTTATATAGTTGAGG+AGG - chr8_1:6151704-6151723 Msa1174920:intron 30.0%
ATTTCACATGTCAAGTTCAA+GGG + chr8_1:6151784-6151803 None:intergenic 30.0%
AATTTCACATGTCAAGTTCA+AGG + chr8_1:6151785-6151804 None:intergenic 30.0%
GAGCTATTCTCTAATGTAAA+GGG - chr8_1:6152297-6152316 Msa1174920:three_prime_UTR 30.0%
CTAATGTAAAGGGTAAAAGT+TGG - chr8_1:6152307-6152326 Msa1174920:three_prime_UTR 30.0%
ATGATAAGATGTTGAAGCTT+TGG + chr8_1:6152375-6152394 None:intergenic 30.0%
TCAACATCTTATCATTCATG+AGG - chr8_1:6152380-6152399 Msa1174920:three_prime_UTR 30.0%
GTTTACAGGAGATTGAAAAT+AGG - chr8_1:6152499-6152518 Msa1174920:CDS 30.0%
AGAGAATGGAAATGATGTTA+AGG - chr8_1:6152524-6152543 Msa1174920:intron 30.0%
GAAATGATGTTAAGGACAAT+TGG - chr8_1:6152532-6152551 Msa1174920:intron 30.0%
! TGCTTGTTGAAGAATTATAG+AGG - chr8_1:6152603-6152622 Msa1174920:CDS 30.0%
TGAAAATGTAAACGACATAG+AGG + chr8_1:6152896-6152915 None:intergenic 30.0%
AAGGAAAAGTAAAAGTTGAG+AGG + chr8_1:6152960-6152979 None:intergenic 30.0%
ATATTATACTCACTCTTTGG+GGG + chr8_1:6151386-6151405 None:intergenic 30.0%
!! CTATATAACAACTTTTGAGG+CGG + chr8_1:6151698-6151717 None:intergenic 30.0%
AAGTTGTTATATAGTTGAGG+AGG - chr8_1:6151704-6151723 Msa1174920:intron 30.0%
ATTTCACATGTCAAGTTCAA+GGG + chr8_1:6151784-6151803 None:intergenic 30.0%
AATTTCACATGTCAAGTTCA+AGG + chr8_1:6151785-6151804 None:intergenic 30.0%
GAGCTATTCTCTAATGTAAA+GGG - chr8_1:6152297-6152316 Msa1174920:three_prime_UTR 30.0%
CTAATGTAAAGGGTAAAAGT+TGG - chr8_1:6152307-6152326 Msa1174920:three_prime_UTR 30.0%
ATGATAAGATGTTGAAGCTT+TGG + chr8_1:6152375-6152394 None:intergenic 30.0%
TCAACATCTTATCATTCATG+AGG - chr8_1:6152380-6152399 Msa1174920:three_prime_UTR 30.0%
GTTTACAGGAGATTGAAAAT+AGG - chr8_1:6152499-6152518 Msa1174920:CDS 30.0%
AGAGAATGGAAATGATGTTA+AGG - chr8_1:6152524-6152543 Msa1174920:intron 30.0%
GAAATGATGTTAAGGACAAT+TGG - chr8_1:6152532-6152551 Msa1174920:intron 30.0%
! TGCTTGTTGAAGAATTATAG+AGG - chr8_1:6152603-6152622 Msa1174920:CDS 30.0%
TGAAAATGTAAACGACATAG+AGG + chr8_1:6152896-6152915 None:intergenic 30.0%
AAGGAAAAGTAAAAGTTGAG+AGG + chr8_1:6152960-6152979 None:intergenic 30.0%
ACTCAAAAGGAGATGAGATT+AGG - chr8_1:6151221-6151240 Msa1174920:three_prime_UTR 35.0%
ACATATAATTGAGTCCGGAA+GGG + chr8_1:6151491-6151510 None:intergenic 35.0%
CAAAGAGATGGAAACCATTA+TGG - chr8_1:6151563-6151582 Msa1174920:intron 35.0%
TAGTGAGAAGAATTCGTGAT+CGG + chr8_1:6151614-6151633 None:intergenic 35.0%
CTTCCAACATATTCACTTGT+AGG - chr8_1:6151807-6151826 Msa1174920:intron 35.0%
TGTCCTACAAGTGAATATGT+TGG + chr8_1:6151813-6151832 None:intergenic 35.0%
TTATTTGAGGAATGTCTCTG+AGG + chr8_1:6151868-6151887 None:intergenic 35.0%
!! GTTTGCAATTCCTCTAGAAT+GGG + chr8_1:6151985-6152004 None:intergenic 35.0%
! TGTTTGCAATTCCTCTAGAA+TGG + chr8_1:6151986-6152005 None:intergenic 35.0%
!!! ACGTAAAGGTACTTCGTTTT+AGG - chr8_1:6152010-6152029 Msa1174920:intron 35.0%
!! TTGATCTTGTCAGCTGTTTT+AGG + chr8_1:6152045-6152064 None:intergenic 35.0%
!! AGGTTTGTTATCAGAAAGCT+TGG + chr8_1:6152091-6152110 None:intergenic 35.0%
CTTTGTAATTCATGCAGACA+TGG - chr8_1:6152229-6152248 Msa1174920:three_prime_UTR 35.0%
GGAGCTATTCTCTAATGTAA+AGG - chr8_1:6152296-6152315 Msa1174920:three_prime_UTR 35.0%
!!! CTTTTGTTTCTTGTTTTGGG+TGG - chr8_1:6152463-6152482 Msa1174920:CDS 35.0%
!!! AAGTTTTGAGTTGAGAAGCA+AGG + chr8_1:6152584-6152603 None:intergenic 35.0%
CAGACAATTTCTGTAACATC+CGG + chr8_1:6152734-6152753 None:intergenic 35.0%
AATATAAGTTTACAGCCCCT+TGG + chr8_1:6152762-6152781 None:intergenic 35.0%
TATATTTGAATGACTAGGGG+AGG - chr8_1:6152776-6152795 Msa1174920:CDS 35.0%
TAGATTCTAGTGCTAGTCAA+TGG - chr8_1:6152837-6152856 Msa1174920:five_prime_UTR 35.0%
ACGACATAGAGGACAAAATA+AGG + chr8_1:6152885-6152904 None:intergenic 35.0%
! AAGTAAAAGTTGAGAGGTAC+TGG + chr8_1:6152954-6152973 None:intergenic 35.0%
ACTCAAAAGGAGATGAGATT+AGG - chr8_1:6151221-6151240 Msa1174920:three_prime_UTR 35.0%
ACATATAATTGAGTCCGGAA+GGG + chr8_1:6151491-6151510 None:intergenic 35.0%
CAAAGAGATGGAAACCATTA+TGG - chr8_1:6151563-6151582 Msa1174920:intron 35.0%
TAGTGAGAAGAATTCGTGAT+CGG + chr8_1:6151614-6151633 None:intergenic 35.0%
CTTCCAACATATTCACTTGT+AGG - chr8_1:6151807-6151826 Msa1174920:intron 35.0%
TGTCCTACAAGTGAATATGT+TGG + chr8_1:6151813-6151832 None:intergenic 35.0%
TTATTTGAGGAATGTCTCTG+AGG + chr8_1:6151868-6151887 None:intergenic 35.0%
!! GTTTGCAATTCCTCTAGAAT+GGG + chr8_1:6151985-6152004 None:intergenic 35.0%
! TGTTTGCAATTCCTCTAGAA+TGG + chr8_1:6151986-6152005 None:intergenic 35.0%
!!! ACGTAAAGGTACTTCGTTTT+AGG - chr8_1:6152010-6152029 Msa1174920:intron 35.0%
!! TTGATCTTGTCAGCTGTTTT+AGG + chr8_1:6152045-6152064 None:intergenic 35.0%
!! AGGTTTGTTATCAGAAAGCT+TGG + chr8_1:6152091-6152110 None:intergenic 35.0%
CTTTGTAATTCATGCAGACA+TGG - chr8_1:6152229-6152248 Msa1174920:three_prime_UTR 35.0%
GGAGCTATTCTCTAATGTAA+AGG - chr8_1:6152296-6152315 Msa1174920:three_prime_UTR 35.0%
!!! CTTTTGTTTCTTGTTTTGGG+TGG - chr8_1:6152463-6152482 Msa1174920:CDS 35.0%
!!! AAGTTTTGAGTTGAGAAGCA+AGG + chr8_1:6152584-6152603 None:intergenic 35.0%
CAGACAATTTCTGTAACATC+CGG + chr8_1:6152734-6152753 None:intergenic 35.0%
AATATAAGTTTACAGCCCCT+TGG + chr8_1:6152762-6152781 None:intergenic 35.0%
TATATTTGAATGACTAGGGG+AGG - chr8_1:6152776-6152795 Msa1174920:CDS 35.0%
TAGATTCTAGTGCTAGTCAA+TGG - chr8_1:6152837-6152856 Msa1174920:five_prime_UTR 35.0%
ACGACATAGAGGACAAAATA+AGG + chr8_1:6152885-6152904 None:intergenic 35.0%
! AAGTAAAAGTTGAGAGGTAC+TGG + chr8_1:6152954-6152973 None:intergenic 35.0%
!! AGCGCTGTTGGCTTTAAATA+AGG + chr8_1:6151177-6151196 None:intergenic 40.0%
!!! CTTTTGAGTAGCGGATTCAA+TGG + chr8_1:6151210-6151229 None:intergenic 40.0%
CATTGAATCCGCTACTCAAA+AGG - chr8_1:6151208-6151227 Msa1174920:three_prime_UTR 40.0%
!! TCTCATCTCCTTTTGAGTAG+CGG + chr8_1:6151219-6151238 None:intergenic 40.0%
GTGTGAATGGTGTTTCAGAT+CGG + chr8_1:6151277-6151296 None:intergenic 40.0%
CGATTAATTGTTGAACGGTG+TGG + chr8_1:6151311-6151330 None:intergenic 40.0%
TAATCCGAAGTTGAATCTCG+TGG + chr8_1:6151358-6151377 None:intergenic 40.0%
GACATATAATTGAGTCCGGA+AGG + chr8_1:6151492-6151511 None:intergenic 40.0%
! TCTCTTTGAGAGAATGCTTG+TGG + chr8_1:6151551-6151570 None:intergenic 40.0%
CAAGCATTCTCTCAAAGAGA+TGG - chr8_1:6151551-6151570 Msa1174920:intron 40.0%
ATAGCATGACTGAGTACATC+TGG + chr8_1:6151644-6151663 None:intergenic 40.0%
ACTACTCAAAACTCTCCACT+TGG - chr8_1:6151904-6151923 Msa1174920:three_prime_UTR 40.0%
!! CAATTCCTCTAGAATGGGAA+AGG + chr8_1:6151980-6151999 None:intergenic 40.0%
GATCAAATGCTTGCCCAATT+TGG - chr8_1:6152059-6152078 Msa1174920:intron 40.0%
AATAGGGACTTGACCATAGT+GGG + chr8_1:6152256-6152275 None:intergenic 40.0%
AAATAGGGACTTGACCATAG+TGG + chr8_1:6152257-6152276 None:intergenic 40.0%
! GTAAAAGTTGGCCTAAGAAG+TGG - chr8_1:6152319-6152338 Msa1174920:three_prime_UTR 40.0%
TGATGTTAAGGACAATTGGG+AGG - chr8_1:6152536-6152555 Msa1174920:intron 40.0%
!! GGCATTCTGGAACAATCTTT+GGG + chr8_1:6152563-6152582 None:intergenic 40.0%
!! AGGCATTCTGGAACAATCTT+TGG + chr8_1:6152564-6152583 None:intergenic 40.0%
AGAAATTGTCTGTGTGTCCA+AGG - chr8_1:6152742-6152761 Msa1174920:CDS 40.0%
GAAATTGTCTGTGTGTCCAA+GGG - chr8_1:6152743-6152762 Msa1174920:CDS 40.0%
AAATTGTCTGTGTGTCCAAG+GGG - chr8_1:6152744-6152763 Msa1174920:CDS 40.0%
!! AAAGTTGAGAGGTACTGGAA+TGG + chr8_1:6152949-6152968 None:intergenic 40.0%
TGAGATGATGCATAGCTACA+TGG - chr8_1:6153055-6153074 Msa1174920:five_prime_UTR 40.0%
TGCATAGCTACATGGATCAA+AGG - chr8_1:6153063-6153082 Msa1174920:five_prime_UTR 40.0%
GCATAGCTACATGGATCAAA+GGG - chr8_1:6153064-6153083 Msa1174920:five_prime_UTR 40.0%
!! AGCGCTGTTGGCTTTAAATA+AGG + chr8_1:6151177-6151196 None:intergenic 40.0%
!!! CTTTTGAGTAGCGGATTCAA+TGG + chr8_1:6151210-6151229 None:intergenic 40.0%
CATTGAATCCGCTACTCAAA+AGG - chr8_1:6151208-6151227 Msa1174920:three_prime_UTR 40.0%
!! TCTCATCTCCTTTTGAGTAG+CGG + chr8_1:6151219-6151238 None:intergenic 40.0%
GTGTGAATGGTGTTTCAGAT+CGG + chr8_1:6151277-6151296 None:intergenic 40.0%
CGATTAATTGTTGAACGGTG+TGG + chr8_1:6151311-6151330 None:intergenic 40.0%
TAATCCGAAGTTGAATCTCG+TGG + chr8_1:6151358-6151377 None:intergenic 40.0%
GACATATAATTGAGTCCGGA+AGG + chr8_1:6151492-6151511 None:intergenic 40.0%
! TCTCTTTGAGAGAATGCTTG+TGG + chr8_1:6151551-6151570 None:intergenic 40.0%
CAAGCATTCTCTCAAAGAGA+TGG - chr8_1:6151551-6151570 Msa1174920:intron 40.0%
ATAGCATGACTGAGTACATC+TGG + chr8_1:6151644-6151663 None:intergenic 40.0%
ACTACTCAAAACTCTCCACT+TGG - chr8_1:6151904-6151923 Msa1174920:three_prime_UTR 40.0%
!! CAATTCCTCTAGAATGGGAA+AGG + chr8_1:6151980-6151999 None:intergenic 40.0%
GATCAAATGCTTGCCCAATT+TGG - chr8_1:6152059-6152078 Msa1174920:intron 40.0%
AATAGGGACTTGACCATAGT+GGG + chr8_1:6152256-6152275 None:intergenic 40.0%
AAATAGGGACTTGACCATAG+TGG + chr8_1:6152257-6152276 None:intergenic 40.0%
! GTAAAAGTTGGCCTAAGAAG+TGG - chr8_1:6152319-6152338 Msa1174920:three_prime_UTR 40.0%
TGATGTTAAGGACAATTGGG+AGG - chr8_1:6152536-6152555 Msa1174920:intron 40.0%
!! GGCATTCTGGAACAATCTTT+GGG + chr8_1:6152563-6152582 None:intergenic 40.0%
!! AGGCATTCTGGAACAATCTT+TGG + chr8_1:6152564-6152583 None:intergenic 40.0%
AGAAATTGTCTGTGTGTCCA+AGG - chr8_1:6152742-6152761 Msa1174920:CDS 40.0%
GAAATTGTCTGTGTGTCCAA+GGG - chr8_1:6152743-6152762 Msa1174920:CDS 40.0%
AAATTGTCTGTGTGTCCAAG+GGG - chr8_1:6152744-6152763 Msa1174920:CDS 40.0%
!! AAAGTTGAGAGGTACTGGAA+TGG + chr8_1:6152949-6152968 None:intergenic 40.0%
TGAGATGATGCATAGCTACA+TGG - chr8_1:6153055-6153074 Msa1174920:five_prime_UTR 40.0%
TGCATAGCTACATGGATCAA+AGG - chr8_1:6153063-6153082 Msa1174920:five_prime_UTR 40.0%
GCATAGCTACATGGATCAAA+GGG - chr8_1:6153064-6153083 Msa1174920:five_prime_UTR 40.0%
! TTCCAATTGAAGAGTGCGAC+AGG - chr8_1:6151458-6151477 Msa1174920:intron 45.0%
! TCCAATTGAAGAGTGCGACA+GGG - chr8_1:6151459-6151478 Msa1174920:intron 45.0%
TTGAGGCGGAGTGATAGTAT+TGG + chr8_1:6151684-6151703 None:intergenic 45.0%
CTTGTCCTTTCCCATTCTAG+AGG - chr8_1:6151972-6151991 Msa1174920:three_prime_UTR 45.0%
AGCTTGGCTGTCACCAAATT+GGG + chr8_1:6152075-6152094 None:intergenic 45.0%
AAGCTTGGCTGTCACCAAAT+TGG + chr8_1:6152076-6152095 None:intergenic 45.0%
GAAAGCAGGAAGAGAGGAAT+AGG + chr8_1:6152111-6152130 None:intergenic 45.0%
ACGGACTCTCAGTATAAAGC+TGG - chr8_1:6152137-6152156 Msa1174920:three_prime_UTR 45.0%
AGCATCCACATCCACTTCTT+AGG + chr8_1:6152333-6152352 None:intergenic 45.0%
! AGTTGAGAAGCAAGGCATTC+TGG + chr8_1:6152576-6152595 None:intergenic 45.0%
! TTCCAATTGAAGAGTGCGAC+AGG - chr8_1:6151458-6151477 Msa1174920:intron 45.0%
! TCCAATTGAAGAGTGCGACA+GGG - chr8_1:6151459-6151478 Msa1174920:intron 45.0%
TTGAGGCGGAGTGATAGTAT+TGG + chr8_1:6151684-6151703 None:intergenic 45.0%
CTTGTCCTTTCCCATTCTAG+AGG - chr8_1:6151972-6151991 Msa1174920:three_prime_UTR 45.0%
AGCTTGGCTGTCACCAAATT+GGG + chr8_1:6152075-6152094 None:intergenic 45.0%
AAGCTTGGCTGTCACCAAAT+TGG + chr8_1:6152076-6152095 None:intergenic 45.0%
GAAAGCAGGAAGAGAGGAAT+AGG + chr8_1:6152111-6152130 None:intergenic 45.0%
ACGGACTCTCAGTATAAAGC+TGG - chr8_1:6152137-6152156 Msa1174920:three_prime_UTR 45.0%
AGCATCCACATCCACTTCTT+AGG + chr8_1:6152333-6152352 None:intergenic 45.0%
! AGTTGAGAAGCAAGGCATTC+TGG + chr8_1:6152576-6152595 None:intergenic 45.0%
!! TGAGTAGCGGATTCAATGGC+TGG + chr8_1:6151206-6151225 None:intergenic 50.0%
! GGTTGAGCTAGAGGTGTGAA+TGG + chr8_1:6151290-6151309 None:intergenic 50.0%
CACGCCACGAGATTCAACTT+CGG - chr8_1:6151351-6151370 Msa1174920:intron 50.0%
! TCGCTGCTGCTTCTTTGACT+TGG + chr8_1:6151431-6151450 None:intergenic 50.0%
CAAAGAAGCAGCAGCGATCA+TGG - chr8_1:6151434-6151453 Msa1174920:intron 50.0%
ACCCTGTCGCACTCTTCAAT+TGG + chr8_1:6151463-6151482 None:intergenic 50.0%
GGTGTCTTTGGTTGGGAGAA+AGG + chr8_1:6151524-6151543 None:intergenic 50.0%
TGAGAGAATGCTTGTGGCGA+CGG + chr8_1:6151545-6151564 None:intergenic 50.0%
GTGAGGACTGAGAGCCATAA+TGG + chr8_1:6151580-6151599 None:intergenic 50.0%
GATCGGTGAAATCGAGAGTG+AGG + chr8_1:6151597-6151616 None:intergenic 50.0%
TCCTCTCTTCCTGCTTTCCA+AGG - chr8_1:6152113-6152132 Msa1174920:three_prime_UTR 50.0%
TCTTCCTGCTTTCCAAGGCA+CGG - chr8_1:6152118-6152137 Msa1174920:three_prime_UTR 50.0%
TATACTGAGAGTCCGTGCCT+TGG + chr8_1:6152133-6152152 None:intergenic 50.0%
ATGCAGACATGGACCCACTA+TGG - chr8_1:6152240-6152259 Msa1174920:three_prime_UTR 50.0%
! GTTGGCCTAAGAAGTGGATG+TGG - chr8_1:6152325-6152344 Msa1174920:three_prime_UTR 50.0%
TTGAATGACTAGGGGAGGCT+AGG - chr8_1:6152781-6152800 Msa1174920:CDS 50.0%
!! TGAGTAGCGGATTCAATGGC+TGG + chr8_1:6151206-6151225 None:intergenic 50.0%
! GGTTGAGCTAGAGGTGTGAA+TGG + chr8_1:6151290-6151309 None:intergenic 50.0%
CACGCCACGAGATTCAACTT+CGG - chr8_1:6151351-6151370 Msa1174920:intron 50.0%
! TCGCTGCTGCTTCTTTGACT+TGG + chr8_1:6151431-6151450 None:intergenic 50.0%
CAAAGAAGCAGCAGCGATCA+TGG - chr8_1:6151434-6151453 Msa1174920:intron 50.0%
ACCCTGTCGCACTCTTCAAT+TGG + chr8_1:6151463-6151482 None:intergenic 50.0%
GGTGTCTTTGGTTGGGAGAA+AGG + chr8_1:6151524-6151543 None:intergenic 50.0%
TGAGAGAATGCTTGTGGCGA+CGG + chr8_1:6151545-6151564 None:intergenic 50.0%
GTGAGGACTGAGAGCCATAA+TGG + chr8_1:6151580-6151599 None:intergenic 50.0%
GATCGGTGAAATCGAGAGTG+AGG + chr8_1:6151597-6151616 None:intergenic 50.0%
TCCTCTCTTCCTGCTTTCCA+AGG - chr8_1:6152113-6152132 Msa1174920:three_prime_UTR 50.0%
TCTTCCTGCTTTCCAAGGCA+CGG - chr8_1:6152118-6152137 Msa1174920:three_prime_UTR 50.0%
TATACTGAGAGTCCGTGCCT+TGG + chr8_1:6152133-6152152 None:intergenic 50.0%
ATGCAGACATGGACCCACTA+TGG - chr8_1:6152240-6152259 Msa1174920:three_prime_UTR 50.0%
! GTTGGCCTAAGAAGTGGATG+TGG - chr8_1:6152325-6152344 Msa1174920:three_prime_UTR 50.0%
TTGAATGACTAGGGGAGGCT+AGG - chr8_1:6152781-6152800 Msa1174920:CDS 50.0%
!! TAGCGGATTCAATGGCTGGC+TGG + chr8_1:6151202-6151221 None:intergenic 55.0%
GAACGGTGTGGTTGAGCTAG+AGG + chr8_1:6151299-6151318 None:intergenic 55.0%
CGAAGTTGAATCTCGTGGCG+TGG + chr8_1:6151353-6151372 None:intergenic 55.0%
TGGCGACGGTGTCTTTGGTT+GGG + chr8_1:6151531-6151550 None:intergenic 55.0%
GCCTTGGAAAGCAGGAAGAG+AGG + chr8_1:6152117-6152136 None:intergenic 55.0%
!! TAGCGGATTCAATGGCTGGC+TGG + chr8_1:6151202-6151221 None:intergenic 55.0%
GAACGGTGTGGTTGAGCTAG+AGG + chr8_1:6151299-6151318 None:intergenic 55.0%
CGAAGTTGAATCTCGTGGCG+TGG + chr8_1:6151353-6151372 None:intergenic 55.0%
TGGCGACGGTGTCTTTGGTT+GGG + chr8_1:6151531-6151550 None:intergenic 55.0%
GCCTTGGAAAGCAGGAAGAG+AGG + chr8_1:6152117-6152136 None:intergenic 55.0%
TTGAATCTCGTGGCGTGGCG+TGG + chr8_1:6151348-6151367 None:intergenic 60.0%
!! CGACAGGGTTAGCTCCCTTC+CGG - chr8_1:6151474-6151493 Msa1174920:intron 60.0%
GTGGCGACGGTGTCTTTGGT+TGG + chr8_1:6151532-6151551 None:intergenic 60.0%
GCTTGTGGCGACGGTGTCTT+TGG + chr8_1:6151536-6151555 None:intergenic 60.0%
GAGTCCGTGCCTTGGAAAGC+AGG + chr8_1:6152125-6152144 None:intergenic 60.0%
TTGAATCTCGTGGCGTGGCG+TGG + chr8_1:6151348-6151367 None:intergenic 60.0%
!! CGACAGGGTTAGCTCCCTTC+CGG - chr8_1:6151474-6151493 Msa1174920:intron 60.0%
GTGGCGACGGTGTCTTTGGT+TGG + chr8_1:6151532-6151551 None:intergenic 60.0%
GCTTGTGGCGACGGTGTCTT+TGG + chr8_1:6151536-6151555 None:intergenic 60.0%
GAGTCCGTGCCTTGGAAAGC+AGG + chr8_1:6152125-6152144 None:intergenic 60.0%
! GGCTGGCTGGCTAGCGCTGT+TGG + chr8_1:6151189-6151208 None:intergenic 70.0%
! GGCTGGCTGGCTAGCGCTGT+TGG + chr8_1:6151189-6151208 None:intergenic 70.0%
TCTCGTGGCGTGGCGTGGCG+TGG + chr8_1:6151343-6151362 None:intergenic 75.0%
TCTCGTGGCGTGGCGTGGCG+TGG + chr8_1:6151343-6151362 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 6151168 6153091 6151168 ID=Msa1174920;Name=Msa1174920
chr8_1 mRNA 6151168 6153091 6151168 ID=Msa1174920-mRNA-1;Parent=Msa1174920;Name=Msa1174920-mRNA-1;_AED=0.18;_eAED=0.18;_QI=256|0.75|0.8|0.8|0.25|0.2|5|545|127
chr8_1 exon 6151168 6151248 6151168 ID=Msa1174920-mRNA-1:exon:2138;Parent=Msa1174920-mRNA-1
chr8_1 exon 6151858 6152013 6151858 ID=Msa1174920-mRNA-1:exon:2137;Parent=Msa1174920-mRNA-1
chr8_1 exon 6152101 6152353 6152101 ID=Msa1174920-mRNA-1:exon:2136;Parent=Msa1174920-mRNA-1
chr8_1 exon 6152376 6152532 6152376 ID=Msa1174920-mRNA-1:exon:2135;Parent=Msa1174920-mRNA-1
chr8_1 exon 6152554 6153091 6152554 ID=Msa1174920-mRNA-1:exon:2134;Parent=Msa1174920-mRNA-1
chr8_1 five_prime_UTR 6152836 6153091 6152836 ID=Msa1174920-mRNA-1:five_prime_utr;Parent=Msa1174920-mRNA-1
chr8_1 CDS 6152554 6152835 6152554 ID=Msa1174920-mRNA-1:cds;Parent=Msa1174920-mRNA-1
chr8_1 CDS 6152431 6152532 6152431 ID=Msa1174920-mRNA-1:cds;Parent=Msa1174920-mRNA-1
chr8_1 three_prime_UTR 6152376 6152430 6152376 ID=Msa1174920-mRNA-1:three_prime_utr;Parent=Msa1174920-mRNA-1
chr8_1 three_prime_UTR 6152101 6152353 6152101 ID=Msa1174920-mRNA-1:three_prime_utr;Parent=Msa1174920-mRNA-1
chr8_1 three_prime_UTR 6151858 6152013 6151858 ID=Msa1174920-mRNA-1:three_prime_utr;Parent=Msa1174920-mRNA-1
chr8_1 three_prime_UTR 6151168 6151248 6151168 ID=Msa1174920-mRNA-1:three_prime_utr;Parent=Msa1174920-mRNA-1
Gene Sequence

>Msa1174920

ATGTCCAAGTCAAAGAAGCAGCAGCGATCATGGATTCCAATTGAAGAGTGCGACAGGGTTAGCTCCCTTCCGGACTCAATTATATGTCATATTCTCTCCTTTCTCCCAACCAAAGACACCGTCGCCACAAGCATTCTCTCAAAGAGATGGAAACCATTATGGCTCTCAGTCCTCACTCTCGATTTCACCGATCACGAATTCTTCTCACTATTTGCAGCCAGATGTACTCAGTCATGCTATCACGCGACAACACACTGCCAATACTATCACTCCGCCTCAAAAAACCCAATGATATCACTCAACTTATTATTGCAGCAATACAAAGACGAACTGAAACCCTTGAACTTGACATGTGAAATTAGCTTCCAACATATTCACTTGTAG
Protein sequence

>Msa1174920

MSKSKKQQRSWIPIEECDRVSSLPDSIICHILSFLPTKDTVATSILSKRWKPLWLSVLTLDFTDHEFFSLFAARCTQSCYHATTHCQYYHSASKNPMISLNLLLQQYKDELKPLNLTCEISFQHIHL*