AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1276900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276900 MtrunA17_Chr3g0120901 53.398 103 44 2 1 103 1 99 2.88e-22 89.0
Msa1276900 MtrunA17_Chr3g0143051 55.882 102 35 4 1 96 1 98 9.14e-22 84.7
Msa1276900 MtrunA17_Chr7g0250271 49.505 101 46 2 1 97 1 100 6.81e-21 81.3
Msa1276900 MtrunA17_Chr0c29g0494011 49.505 101 46 2 1 97 1 100 6.81e-21 81.3
Msa1276900 MtrunA17_Chr3g0142561 49.000 100 43 3 1 96 1 96 6.39e-16 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCAAATGCTTGCCCAATT+TGG 0.243250 8_3:-11848723 Msa1276900:three_prime_UTR
ATAATATTATACTCACTCTT+TGG 0.276797 8_3:+11849359 None:intergenic
AAGCTTGGCTGTCACCAAAT+TGG 0.276858 8_3:+11848709 None:intergenic
TAATATTATACTCACTCTTT+GGG 0.278133 8_3:+11849360 None:intergenic
CTTCCAACATATTCACTTGT+AGG 0.295079 8_3:-11848953 Msa1276900:exon
AGCGCTGTTGGCTTTAAATA+AGG 0.309434 8_3:+11849556 None:intergenic
GCTTGTGGCGACGGTGTCTT+TGG 0.336244 8_3:+11849210 None:intergenic
GGCTGGCTGGCTAGCGCTGT+TGG 0.346233 8_3:+11849544 None:intergenic
GAAAGCAGGAAGAGAGGAAT+AGG 0.350371 8_3:+11848674 None:intergenic
AGCATCCACATCCACTTCTT+AGG 0.366035 8_3:+11848447 None:intergenic
AGCTTGGCTGTCACCAAATT+GGG 0.366685 8_3:+11848710 None:intergenic
AGATGGAAACCATACCATTA+TGG 0.384713 8_3:-11849175 Msa1276900:CDS
TGTTTGCAATTCCTCTAGAA+TGG 0.403902 8_3:+11848799 None:intergenic
ATGATAAGATGTTGAAGCTT+TGG 0.408194 8_3:+11848405 None:intergenic
GGTGTCTTTGGTTGGGAGAA+AGG 0.415987 8_3:+11849222 None:intergenic
AATAAGTTCAGTGATATCAT+TGG 0.420860 8_3:+11849030 None:intergenic
GAGCTATTCTCTAATGTAAA+GGG 0.420946 8_3:-11848480 Msa1276900:three_prime_UTR
AATATTTAATAAACAACTGC+TGG 0.423210 8_3:+11847739 None:intergenic
ACCCTGTCGCACTCTTCAAT+TGG 0.424294 8_3:+11849285 None:intergenic
CGACAGGGTTAGCTCCCTTC+CGG 0.429890 8_3:-11849271 Msa1276900:CDS
CAAAGAAGCAGCAGCGATCA+TGG 0.433969 8_3:-11849311 Msa1276900:CDS
ATAAGTTCAGTGATATCATT+GGG 0.438314 8_3:+11849031 None:intergenic
TCGCTGCTGCTTCTTTGACT+TGG 0.438632 8_3:+11849317 None:intergenic
TTAATTTCAACACTCATAAA+CGG 0.440294 8_3:-11848847 Msa1276900:three_prime_UTR
GTGAGGACTGAGAGCCATAA+TGG 0.442513 8_3:+11849161 None:intergenic
TCCTCTCTTCCTGCTTTCCA+AGG 0.442960 8_3:-11848669 Msa1276900:three_prime_UTR
GTTTGCAATTCCTCTAGAAT+GGG 0.446597 8_3:+11848800 None:intergenic
GTGGCGACGGTGTCTTTGGT+TGG 0.449866 8_3:+11849214 None:intergenic
AATTTCACATGTCAAGTTCA+AGG 0.456173 8_3:+11848978 None:intergenic
TGGCGACGGTGTCTTTGGTT+GGG 0.461534 8_3:+11849215 None:intergenic
ATTGCAAACAAATTACGTTA+AGG 0.463433 8_3:-11848786 Msa1276900:three_prime_UTR
TGAGTAGCGGATTCAATGGC+TGG 0.463741 8_3:+11849523 None:intergenic
AAATGGGGACTTGGACATAG+TGG 0.464992 8_3:+11848523 None:intergenic
TTCCAATTGAAGAGTGCGAC+AGG 0.466848 8_3:-11849287 Msa1276900:CDS
GGAGCTATTCTCTAATGTAA+AGG 0.470359 8_3:-11848481 Msa1276900:three_prime_UTR
ACTCAAAAGGAGATGAGATT+AGG 0.473899 8_3:-11849505 Msa1276900:five_prime_UTR
GAGTCCGTGCCTTGGAAAGC+AGG 0.474538 8_3:+11848660 None:intergenic
CATTGAATCCGCTACTCAAA+AGG 0.477575 8_3:-11849518 Msa1276900:five_prime_UTR
GGATTCAATGGCTGGCTGGC+TGG 0.485867 8_3:+11849531 None:intergenic
ATAGCATGACTGAGTACATC+TGG 0.496230 8_3:+11849097 None:intergenic
AGGTTTGTTATCAGAAAGCT+TGG 0.498630 8_3:+11848694 None:intergenic
CTTGTCCTTTCCCATTCTAG+AGG 0.504356 8_3:-11848810 Msa1276900:three_prime_UTR
TAGCGGATTCAATGGCTGGC+TGG 0.506161 8_3:+11849527 None:intergenic
CTAATGTAAAGGGTAAAACT+TGG 0.507647 8_3:-11848470 Msa1276900:three_prime_UTR
GCATAGCTACATGGATCAAA+GGG 0.521045 8_3:-11847708 Msa1276900:three_prime_UTR
AATATTATACTCACTCTTTG+GGG 0.525869 8_3:+11849361 None:intergenic
TGAGAGAATGCTTGTGGCGA+CGG 0.533104 8_3:+11849201 None:intergenic
TCTTCCTGCTTTCCAAGGCA+CGG 0.537877 8_3:-11848664 Msa1276900:three_prime_UTR
ATTTCACATGTCAAGTTCAA+GGG 0.540030 8_3:+11848979 None:intergenic
CAAGCATTCTCTCAAAGAGA+TGG 0.543124 8_3:-11849192 Msa1276900:CDS
TGCATAGCTACATGGATCAA+AGG 0.543192 8_3:-11847709 Msa1276900:three_prime_UTR
ATATGACATATAATTGAGTC+CGG 0.544933 8_3:+11849252 None:intergenic
GGTTGAGCTAGAGGTGTGAA+TGG 0.545726 8_3:+11849439 None:intergenic
GTAAAACTTGGCCTAAGAAG+TGG 0.549284 8_3:-11848458 Msa1276900:three_prime_UTR
GACATATAATTGAGTCCGGA+AGG 0.551664 8_3:+11849256 None:intergenic
TATACTGAGAGTCCGTGCCT+TGG 0.551730 8_3:+11848652 None:intergenic
CAATTCCTCTAGAATGGGAA+AGG 0.553185 8_3:+11848805 None:intergenic
TAACGGTGTGGTTGAGCTAG+AGG 0.553983 8_3:+11849430 None:intergenic
GACTGAGAGCCATAATGGTA+TGG 0.554163 8_3:+11849166 None:intergenic
TGAGATGATGCATAGCTACA+TGG 0.555182 8_3:-11847717 Msa1276900:three_prime_UTR
ACTACTCAAAACTCTCCACT+TGG 0.555992 8_3:-11848878 Msa1276900:three_prime_UTR
TCAACATCTTATCATTCATG+AGG 0.570247 8_3:-11848397 Msa1276900:intron
GCCTTGGAAAGCAGGAAGAG+AGG 0.573004 8_3:+11848668 None:intergenic
CACGCCACGAGATTCAGCTT+CGG 0.573687 8_3:-11849394 Msa1276900:five_prime_UTR
ACGGACTCTCAGTATAAAGC+TGG 0.574567 8_3:-11848645 Msa1276900:intron
CTTGGCCTAAGAAGTGGATG+TGG 0.584782 8_3:-11848452 Msa1276900:three_prime_UTR
TGTCCTACAAGTGAATATGT+TGG 0.588152 8_3:+11848950 None:intergenic
TTGAGGCGGAGTGATAGTAT+TGG 0.589104 8_3:+11849057 None:intergenic
GTGTGAATGGTGTTTCAGAT+CGG 0.595902 8_3:+11849452 None:intergenic
TCTCTTTGAGAGAATGCTTG+TGG 0.597896 8_3:+11849195 None:intergenic
AATGGGGACTTGGACATAGT+GGG 0.607464 8_3:+11848524 None:intergenic
ATATTATACTCACTCTTTGG+GGG 0.612790 8_3:+11849362 None:intergenic
TAGTGAGAAGAATTCGTGAT+CGG 0.621496 8_3:+11849127 None:intergenic
ACATATAATTGAGTCCGGAA+GGG 0.623798 8_3:+11849257 None:intergenic
CGTGGTGTTTGAATCGATAA+CGG 0.628502 8_3:+11849413 None:intergenic
GATCGGTGAAATCGAGAGTG+AGG 0.633584 8_3:+11849144 None:intergenic
TCCAATTGAAGAGTGCGACA+GGG 0.638921 8_3:-11849286 Msa1276900:CDS
CGAAGCTGAATCTCGTGGCG+TGG 0.653196 8_3:+11849395 None:intergenic
AGTAGTGAAGTATGTCTCTG+AGG 0.657114 8_3:+11848895 None:intergenic
AAATTAACACAAAATCCAAG+TGG 0.668914 8_3:+11848863 None:intergenic
TAATCCGAAGCTGAATCTCG+TGG 0.686017 8_3:+11849390 None:intergenic
TGTTTGAATCGATAACGGTG+TGG 0.721036 8_3:+11849418 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACATTTTCAATTATGTAT+TGG - chr8_3:11849398-11849417 Msa1276900:five_prime_UTR 15.0%
!!! TTACATTTTCAATTATGTAT+TGG - chr8_3:11849398-11849417 Msa1276900:five_prime_UTR 15.0%
!! TAATATTATACTCACTCTTT+GGG + chr8_3:11847908-11847927 None:intergenic 20.0%
!! ATAATATTATACTCACTCTT+TGG + chr8_3:11847909-11847928 None:intergenic 20.0%
!! TTAATTTCAACACTCATAAA+CGG - chr8_3:11848418-11848437 Msa1276900:three_prime_UTR 20.0%
!! TGAGTTAGATTGTAAAAAAT+GGG + chr8_3:11848761-11848780 None:intergenic 20.0%
!! TTGAGTTAGATTGTAAAAAA+TGG + chr8_3:11848762-11848781 None:intergenic 20.0%
!!! TTTTTACAATCTAACTCAAA+TGG - chr8_3:11848763-11848782 Msa1276900:three_prime_UTR 20.0%
!!! TTAACTTTTGTTTCTTCTTT+TGG - chr8_3:11848952-11848971 Msa1276900:exon 20.0%
!!! TAACTTTTGTTTCTTCTTTT+GGG - chr8_3:11848953-11848972 Msa1276900:exon 20.0%
!!! GAATTTTACTTATTGTTTAC+AGG - chr8_3:11848978-11848997 Msa1276900:CDS 20.0%
!!! GTATTTTGCATTTAAATCTA+TGG + chr8_3:11849205-11849224 None:intergenic 20.0%
!! TAGATTTAAATGCAAAATAC+CGG - chr8_3:11849205-11849224 Msa1276900:CDS 20.0%
!! AAACTTATATTTGAATGACT+AGG - chr8_3:11849264-11849283 Msa1276900:CDS 20.0%
!! AACTTATATTTGAATGACTA+GGG - chr8_3:11849265-11849284 Msa1276900:CDS 20.0%
!! AATATTTAATAAACAACTGC+TGG + chr8_3:11849529-11849548 None:intergenic 20.0%
!! TAATATTATACTCACTCTTT+GGG + chr8_3:11847908-11847927 None:intergenic 20.0%
!! ATAATATTATACTCACTCTT+TGG + chr8_3:11847909-11847928 None:intergenic 20.0%
!! TTAATTTCAACACTCATAAA+CGG - chr8_3:11848418-11848437 Msa1276900:three_prime_UTR 20.0%
!! TGAGTTAGATTGTAAAAAAT+GGG + chr8_3:11848761-11848780 None:intergenic 20.0%
!! TTGAGTTAGATTGTAAAAAA+TGG + chr8_3:11848762-11848781 None:intergenic 20.0%
!!! TTTTTACAATCTAACTCAAA+TGG - chr8_3:11848763-11848782 Msa1276900:three_prime_UTR 20.0%
!!! TTAACTTTTGTTTCTTCTTT+TGG - chr8_3:11848952-11848971 Msa1276900:exon 20.0%
!!! TAACTTTTGTTTCTTCTTTT+GGG - chr8_3:11848953-11848972 Msa1276900:exon 20.0%
!!! GAATTTTACTTATTGTTTAC+AGG - chr8_3:11848978-11848997 Msa1276900:CDS 20.0%
!!! GTATTTTGCATTTAAATCTA+TGG + chr8_3:11849205-11849224 None:intergenic 20.0%
!! TAGATTTAAATGCAAAATAC+CGG - chr8_3:11849205-11849224 Msa1276900:CDS 20.0%
!! AAACTTATATTTGAATGACT+AGG - chr8_3:11849264-11849283 Msa1276900:CDS 20.0%
!! AACTTATATTTGAATGACTA+GGG - chr8_3:11849265-11849284 Msa1276900:CDS 20.0%
!! AATATTTAATAAACAACTGC+TGG + chr8_3:11849529-11849548 None:intergenic 20.0%
! AATATTATACTCACTCTTTG+GGG + chr8_3:11847907-11847926 None:intergenic 25.0%
! ATATGACATATAATTGAGTC+CGG + chr8_3:11848016-11848035 None:intergenic 25.0%
! ATAAGTTCAGTGATATCATT+GGG + chr8_3:11848237-11848256 None:intergenic 25.0%
! AATAAGTTCAGTGATATCAT+TGG + chr8_3:11848238-11848257 None:intergenic 25.0%
! AAATTAACACAAAATCCAAG+TGG + chr8_3:11848405-11848424 None:intergenic 25.0%
! ATTGCAAACAAATTACGTTA+AGG - chr8_3:11848479-11848498 Msa1276900:three_prime_UTR 25.0%
! GAGTTAGATTGTAAAAAATG+GGG + chr8_3:11848760-11848779 None:intergenic 25.0%
! ACAAAAGTTAAGATATGTAG+AGG + chr8_3:11848943-11848962 None:intergenic 25.0%
! ATTGAAAATAGGATAGAGAA+TGG - chr8_3:11849003-11849022 Msa1276900:CDS 25.0%
! AAATGATGTTAAGGACAATT+GGG - chr8_3:11849026-11849045 Msa1276900:CDS 25.0%
! ACTTATATTTGAATGACTAG+GGG - chr8_3:11849266-11849285 Msa1276900:CDS 25.0%
! ACAATCAGTAAGAAAAAGAA+AGG + chr8_3:11849472-11849491 None:intergenic 25.0%
! AATATTATACTCACTCTTTG+GGG + chr8_3:11847907-11847926 None:intergenic 25.0%
! ATATGACATATAATTGAGTC+CGG + chr8_3:11848016-11848035 None:intergenic 25.0%
! ATAAGTTCAGTGATATCATT+GGG + chr8_3:11848237-11848256 None:intergenic 25.0%
! AATAAGTTCAGTGATATCAT+TGG + chr8_3:11848238-11848257 None:intergenic 25.0%
! AAATTAACACAAAATCCAAG+TGG + chr8_3:11848405-11848424 None:intergenic 25.0%
! ATTGCAAACAAATTACGTTA+AGG - chr8_3:11848479-11848498 Msa1276900:three_prime_UTR 25.0%
! GAGTTAGATTGTAAAAAATG+GGG + chr8_3:11848760-11848779 None:intergenic 25.0%
! ACAAAAGTTAAGATATGTAG+AGG + chr8_3:11848943-11848962 None:intergenic 25.0%
! ATTGAAAATAGGATAGAGAA+TGG - chr8_3:11849003-11849022 Msa1276900:CDS 25.0%
! AAATGATGTTAAGGACAATT+GGG - chr8_3:11849026-11849045 Msa1276900:CDS 25.0%
! ACTTATATTTGAATGACTAG+GGG - chr8_3:11849266-11849285 Msa1276900:CDS 25.0%
! ACAATCAGTAAGAAAAAGAA+AGG + chr8_3:11849472-11849491 None:intergenic 25.0%
ATATTATACTCACTCTTTGG+GGG + chr8_3:11847906-11847925 None:intergenic 30.0%
ATTTCACATGTCAAGTTCAA+GGG + chr8_3:11848289-11848308 None:intergenic 30.0%
AATTTCACATGTCAAGTTCA+AGG + chr8_3:11848290-11848309 None:intergenic 30.0%
GAGCTATTCTCTAATGTAAA+GGG - chr8_3:11848785-11848804 Msa1276900:three_prime_UTR 30.0%
CTAATGTAAAGGGTAAAACT+TGG - chr8_3:11848795-11848814 Msa1276900:three_prime_UTR 30.0%
ATGATAAGATGTTGAAGCTT+TGG + chr8_3:11848863-11848882 None:intergenic 30.0%
TCAACATCTTATCATTCATG+AGG - chr8_3:11848868-11848887 Msa1276900:three_prime_UTR 30.0%
ATCTTATCATTCATGAGGTT+AGG - chr8_3:11848873-11848892 Msa1276900:three_prime_UTR 30.0%
GTTTACAGGAGATTGAAAAT+AGG - chr8_3:11848992-11849011 Msa1276900:CDS 30.0%
AGAGAATGGAAATGATGTTA+AGG - chr8_3:11849017-11849036 Msa1276900:CDS 30.0%
GAAATGATGTTAAGGACAAT+TGG - chr8_3:11849025-11849044 Msa1276900:CDS 30.0%
! TGCTTGTTGAAGAATTATAG+AGG - chr8_3:11849096-11849115 Msa1276900:CDS 30.0%
TGAAAATGTAAACGACATAG+AGG + chr8_3:11849389-11849408 None:intergenic 30.0%
AAGGAAAAGTAAAAGTTGAG+AGG + chr8_3:11849453-11849472 None:intergenic 30.0%
ATATTATACTCACTCTTTGG+GGG + chr8_3:11847906-11847925 None:intergenic 30.0%
ATTTCACATGTCAAGTTCAA+GGG + chr8_3:11848289-11848308 None:intergenic 30.0%
AATTTCACATGTCAAGTTCA+AGG + chr8_3:11848290-11848309 None:intergenic 30.0%
GAGCTATTCTCTAATGTAAA+GGG - chr8_3:11848785-11848804 Msa1276900:three_prime_UTR 30.0%
CTAATGTAAAGGGTAAAACT+TGG - chr8_3:11848795-11848814 Msa1276900:three_prime_UTR 30.0%
ATGATAAGATGTTGAAGCTT+TGG + chr8_3:11848863-11848882 None:intergenic 30.0%
TCAACATCTTATCATTCATG+AGG - chr8_3:11848868-11848887 Msa1276900:three_prime_UTR 30.0%
ATCTTATCATTCATGAGGTT+AGG - chr8_3:11848873-11848892 Msa1276900:three_prime_UTR 30.0%
GTTTACAGGAGATTGAAAAT+AGG - chr8_3:11848992-11849011 Msa1276900:CDS 30.0%
AGAGAATGGAAATGATGTTA+AGG - chr8_3:11849017-11849036 Msa1276900:CDS 30.0%
GAAATGATGTTAAGGACAAT+TGG - chr8_3:11849025-11849044 Msa1276900:CDS 30.0%
! TGCTTGTTGAAGAATTATAG+AGG - chr8_3:11849096-11849115 Msa1276900:CDS 30.0%
TGAAAATGTAAACGACATAG+AGG + chr8_3:11849389-11849408 None:intergenic 30.0%
AAGGAAAAGTAAAAGTTGAG+AGG + chr8_3:11849453-11849472 None:intergenic 30.0%
ACTCAAAAGGAGATGAGATT+AGG - chr8_3:11847760-11847779 Msa1276900:three_prime_UTR 35.0%
ACATATAATTGAGTCCGGAA+GGG + chr8_3:11848011-11848030 None:intergenic 35.0%
AGATGGAAACCATACCATTA+TGG - chr8_3:11848090-11848109 Msa1276900:intron 35.0%
TAGTGAGAAGAATTCGTGAT+CGG + chr8_3:11848141-11848160 None:intergenic 35.0%
!! ATATCATTGGGTTTTTGAGG+CGG + chr8_3:11848225-11848244 None:intergenic 35.0%
! GTGATATCATTGGGTTTTTG+AGG + chr8_3:11848228-11848247 None:intergenic 35.0%
CTTCCAACATATTCACTTGT+AGG - chr8_3:11848312-11848331 Msa1276900:intron 35.0%
TGTCCTACAAGTGAATATGT+TGG + chr8_3:11848318-11848337 None:intergenic 35.0%
!! GTTTGCAATTCCTCTAGAAT+GGG + chr8_3:11848468-11848487 None:intergenic 35.0%
! TGTTTGCAATTCCTCTAGAA+TGG + chr8_3:11848469-11848488 None:intergenic 35.0%
!! ACGTTAAGGTACTTCGTTTT+AGG - chr8_3:11848493-11848512 Msa1276900:three_prime_UTR 35.0%
!! TTGATCTTGTCAGCTGTTTT+AGG + chr8_3:11848528-11848547 None:intergenic 35.0%
!! AGGTTTGTTATCAGAAAGCT+TGG + chr8_3:11848574-11848593 None:intergenic 35.0%
CTTTGTAATTCATGCAGACA+TGG - chr8_3:11848717-11848736 Msa1276900:three_prime_UTR 35.0%
GATTGTAAAAAATGGGGACT+TGG + chr8_3:11848754-11848773 None:intergenic 35.0%
GGAGCTATTCTCTAATGTAA+AGG - chr8_3:11848784-11848803 Msa1276900:three_prime_UTR 35.0%
!! CTTTTGTTTCTTCTTTTGGG+TGG - chr8_3:11848956-11848975 Msa1276900:CDS 35.0%
!!! AAGTTTTGAGTTGAGAAGCA+AGG + chr8_3:11849077-11849096 None:intergenic 35.0%
CAGACAATTTCTGTAACATC+CGG + chr8_3:11849227-11849246 None:intergenic 35.0%
AATATAAGTTTACAGCCCCT+TGG + chr8_3:11849255-11849274 None:intergenic 35.0%
TATATTTGAATGACTAGGGG+AGG - chr8_3:11849269-11849288 Msa1276900:CDS 35.0%
TAGATTCTAGTGCTAGTCAA+TGG - chr8_3:11849330-11849349 Msa1276900:exon 35.0%
ACGACATAGAGGACAAAATA+AGG + chr8_3:11849378-11849397 None:intergenic 35.0%
! AAGTAAAAGTTGAGAGGTAC+TGG + chr8_3:11849447-11849466 None:intergenic 35.0%
ACTCAAAAGGAGATGAGATT+AGG - chr8_3:11847760-11847779 Msa1276900:three_prime_UTR 35.0%
ACATATAATTGAGTCCGGAA+GGG + chr8_3:11848011-11848030 None:intergenic 35.0%
AGATGGAAACCATACCATTA+TGG - chr8_3:11848090-11848109 Msa1276900:intron 35.0%
TAGTGAGAAGAATTCGTGAT+CGG + chr8_3:11848141-11848160 None:intergenic 35.0%
!! ATATCATTGGGTTTTTGAGG+CGG + chr8_3:11848225-11848244 None:intergenic 35.0%
! GTGATATCATTGGGTTTTTG+AGG + chr8_3:11848228-11848247 None:intergenic 35.0%
CTTCCAACATATTCACTTGT+AGG - chr8_3:11848312-11848331 Msa1276900:intron 35.0%
TGTCCTACAAGTGAATATGT+TGG + chr8_3:11848318-11848337 None:intergenic 35.0%
!! GTTTGCAATTCCTCTAGAAT+GGG + chr8_3:11848468-11848487 None:intergenic 35.0%
! TGTTTGCAATTCCTCTAGAA+TGG + chr8_3:11848469-11848488 None:intergenic 35.0%
!! ACGTTAAGGTACTTCGTTTT+AGG - chr8_3:11848493-11848512 Msa1276900:three_prime_UTR 35.0%
!! TTGATCTTGTCAGCTGTTTT+AGG + chr8_3:11848528-11848547 None:intergenic 35.0%
!! AGGTTTGTTATCAGAAAGCT+TGG + chr8_3:11848574-11848593 None:intergenic 35.0%
CTTTGTAATTCATGCAGACA+TGG - chr8_3:11848717-11848736 Msa1276900:three_prime_UTR 35.0%
GATTGTAAAAAATGGGGACT+TGG + chr8_3:11848754-11848773 None:intergenic 35.0%
GGAGCTATTCTCTAATGTAA+AGG - chr8_3:11848784-11848803 Msa1276900:three_prime_UTR 35.0%
!! CTTTTGTTTCTTCTTTTGGG+TGG - chr8_3:11848956-11848975 Msa1276900:CDS 35.0%
!!! AAGTTTTGAGTTGAGAAGCA+AGG + chr8_3:11849077-11849096 None:intergenic 35.0%
CAGACAATTTCTGTAACATC+CGG + chr8_3:11849227-11849246 None:intergenic 35.0%
AATATAAGTTTACAGCCCCT+TGG + chr8_3:11849255-11849274 None:intergenic 35.0%
TATATTTGAATGACTAGGGG+AGG - chr8_3:11849269-11849288 Msa1276900:CDS 35.0%
TAGATTCTAGTGCTAGTCAA+TGG - chr8_3:11849330-11849349 Msa1276900:exon 35.0%
ACGACATAGAGGACAAAATA+AGG + chr8_3:11849378-11849397 None:intergenic 35.0%
! AAGTAAAAGTTGAGAGGTAC+TGG + chr8_3:11849447-11849466 None:intergenic 35.0%
!! AGCGCTGTTGGCTTTAAATA+AGG + chr8_3:11847712-11847731 None:intergenic 40.0%
!!! CTTTTGAGTAGCGGATTCAA+TGG + chr8_3:11847749-11847768 None:intergenic 40.0%
CATTGAATCCGCTACTCAAA+AGG - chr8_3:11847747-11847766 Msa1276900:three_prime_UTR 40.0%
!! TCTCATCTCCTTTTGAGTAG+CGG + chr8_3:11847758-11847777 None:intergenic 40.0%
GTGTGAATGGTGTTTCAGAT+CGG + chr8_3:11847816-11847835 None:intergenic 40.0%
! TGTTTGAATCGATAACGGTG+TGG + chr8_3:11847850-11847869 None:intergenic 40.0%
CGTGGTGTTTGAATCGATAA+CGG + chr8_3:11847855-11847874 None:intergenic 40.0%
GACATATAATTGAGTCCGGA+AGG + chr8_3:11848012-11848031 None:intergenic 40.0%
! TCTCTTTGAGAGAATGCTTG+TGG + chr8_3:11848073-11848092 None:intergenic 40.0%
CAAGCATTCTCTCAAAGAGA+TGG - chr8_3:11848073-11848092 Msa1276900:intron 40.0%
ATAGCATGACTGAGTACATC+TGG + chr8_3:11848171-11848190 None:intergenic 40.0%
AGTAGTGAAGTATGTCTCTG+AGG + chr8_3:11848373-11848392 None:intergenic 40.0%
ACTACTCAAAACTCTCCACT+TGG - chr8_3:11848387-11848406 Msa1276900:intron 40.0%
!! CAATTCCTCTAGAATGGGAA+AGG + chr8_3:11848463-11848482 None:intergenic 40.0%
GATCAAATGCTTGCCCAATT+TGG - chr8_3:11848542-11848561 Msa1276900:intron 40.0%
GTAAAACTTGGCCTAAGAAG+TGG - chr8_3:11848807-11848826 Msa1276900:three_prime_UTR 40.0%
TGATGTTAAGGACAATTGGG+AGG - chr8_3:11849029-11849048 Msa1276900:CDS 40.0%
!! GGCATTCTGGAACAATCTTT+GGG + chr8_3:11849056-11849075 None:intergenic 40.0%
!! AGGCATTCTGGAACAATCTT+TGG + chr8_3:11849057-11849076 None:intergenic 40.0%
AGAAATTGTCTGTGTGTCCA+AGG - chr8_3:11849235-11849254 Msa1276900:CDS 40.0%
GAAATTGTCTGTGTGTCCAA+GGG - chr8_3:11849236-11849255 Msa1276900:CDS 40.0%
AAATTGTCTGTGTGTCCAAG+GGG - chr8_3:11849237-11849256 Msa1276900:CDS 40.0%
!! AAAGTTGAGAGGTACTGGAA+TGG + chr8_3:11849442-11849461 None:intergenic 40.0%
TGAGATGATGCATAGCTACA+TGG - chr8_3:11849548-11849567 Msa1276900:five_prime_UTR 40.0%
TGCATAGCTACATGGATCAA+AGG - chr8_3:11849556-11849575 Msa1276900:five_prime_UTR 40.0%
GCATAGCTACATGGATCAAA+GGG - chr8_3:11849557-11849576 Msa1276900:five_prime_UTR 40.0%
!! AGCGCTGTTGGCTTTAAATA+AGG + chr8_3:11847712-11847731 None:intergenic 40.0%
!!! CTTTTGAGTAGCGGATTCAA+TGG + chr8_3:11847749-11847768 None:intergenic 40.0%
CATTGAATCCGCTACTCAAA+AGG - chr8_3:11847747-11847766 Msa1276900:three_prime_UTR 40.0%
!! TCTCATCTCCTTTTGAGTAG+CGG + chr8_3:11847758-11847777 None:intergenic 40.0%
GTGTGAATGGTGTTTCAGAT+CGG + chr8_3:11847816-11847835 None:intergenic 40.0%
! TGTTTGAATCGATAACGGTG+TGG + chr8_3:11847850-11847869 None:intergenic 40.0%
CGTGGTGTTTGAATCGATAA+CGG + chr8_3:11847855-11847874 None:intergenic 40.0%
GACATATAATTGAGTCCGGA+AGG + chr8_3:11848012-11848031 None:intergenic 40.0%
! TCTCTTTGAGAGAATGCTTG+TGG + chr8_3:11848073-11848092 None:intergenic 40.0%
CAAGCATTCTCTCAAAGAGA+TGG - chr8_3:11848073-11848092 Msa1276900:intron 40.0%
ATAGCATGACTGAGTACATC+TGG + chr8_3:11848171-11848190 None:intergenic 40.0%
AGTAGTGAAGTATGTCTCTG+AGG + chr8_3:11848373-11848392 None:intergenic 40.0%
ACTACTCAAAACTCTCCACT+TGG - chr8_3:11848387-11848406 Msa1276900:intron 40.0%
!! CAATTCCTCTAGAATGGGAA+AGG + chr8_3:11848463-11848482 None:intergenic 40.0%
GATCAAATGCTTGCCCAATT+TGG - chr8_3:11848542-11848561 Msa1276900:intron 40.0%
GTAAAACTTGGCCTAAGAAG+TGG - chr8_3:11848807-11848826 Msa1276900:three_prime_UTR 40.0%
TGATGTTAAGGACAATTGGG+AGG - chr8_3:11849029-11849048 Msa1276900:CDS 40.0%
!! GGCATTCTGGAACAATCTTT+GGG + chr8_3:11849056-11849075 None:intergenic 40.0%
!! AGGCATTCTGGAACAATCTT+TGG + chr8_3:11849057-11849076 None:intergenic 40.0%
AGAAATTGTCTGTGTGTCCA+AGG - chr8_3:11849235-11849254 Msa1276900:CDS 40.0%
GAAATTGTCTGTGTGTCCAA+GGG - chr8_3:11849236-11849255 Msa1276900:CDS 40.0%
AAATTGTCTGTGTGTCCAAG+GGG - chr8_3:11849237-11849256 Msa1276900:CDS 40.0%
!! AAAGTTGAGAGGTACTGGAA+TGG + chr8_3:11849442-11849461 None:intergenic 40.0%
TGAGATGATGCATAGCTACA+TGG - chr8_3:11849548-11849567 Msa1276900:five_prime_UTR 40.0%
TGCATAGCTACATGGATCAA+AGG - chr8_3:11849556-11849575 Msa1276900:five_prime_UTR 40.0%
GCATAGCTACATGGATCAAA+GGG - chr8_3:11849557-11849576 Msa1276900:five_prime_UTR 40.0%
TAATCCGAAGCTGAATCTCG+TGG + chr8_3:11847878-11847897 None:intergenic 45.0%
! TTCCAATTGAAGAGTGCGAC+AGG - chr8_3:11847978-11847997 Msa1276900:intron 45.0%
! TCCAATTGAAGAGTGCGACA+GGG - chr8_3:11847979-11847998 Msa1276900:intron 45.0%
GACTGAGAGCCATAATGGTA+TGG + chr8_3:11848102-11848121 None:intergenic 45.0%
TTGAGGCGGAGTGATAGTAT+TGG + chr8_3:11848211-11848230 None:intergenic 45.0%
CTTGTCCTTTCCCATTCTAG+AGG - chr8_3:11848455-11848474 Msa1276900:three_prime_UTR 45.0%
AGCTTGGCTGTCACCAAATT+GGG + chr8_3:11848558-11848577 None:intergenic 45.0%
AAGCTTGGCTGTCACCAAAT+TGG + chr8_3:11848559-11848578 None:intergenic 45.0%
GAAAGCAGGAAGAGAGGAAT+AGG + chr8_3:11848594-11848613 None:intergenic 45.0%
ACGGACTCTCAGTATAAAGC+TGG - chr8_3:11848620-11848639 Msa1276900:intron 45.0%
AATGGGGACTTGGACATAGT+GGG + chr8_3:11848744-11848763 None:intergenic 45.0%
AAATGGGGACTTGGACATAG+TGG + chr8_3:11848745-11848764 None:intergenic 45.0%
AGCATCCACATCCACTTCTT+AGG + chr8_3:11848821-11848840 None:intergenic 45.0%
! AGTTGAGAAGCAAGGCATTC+TGG + chr8_3:11849069-11849088 None:intergenic 45.0%
TAATCCGAAGCTGAATCTCG+TGG + chr8_3:11847878-11847897 None:intergenic 45.0%
! TTCCAATTGAAGAGTGCGAC+AGG - chr8_3:11847978-11847997 Msa1276900:intron 45.0%
! TCCAATTGAAGAGTGCGACA+GGG - chr8_3:11847979-11847998 Msa1276900:intron 45.0%
GACTGAGAGCCATAATGGTA+TGG + chr8_3:11848102-11848121 None:intergenic 45.0%
TTGAGGCGGAGTGATAGTAT+TGG + chr8_3:11848211-11848230 None:intergenic 45.0%
CTTGTCCTTTCCCATTCTAG+AGG - chr8_3:11848455-11848474 Msa1276900:three_prime_UTR 45.0%
AGCTTGGCTGTCACCAAATT+GGG + chr8_3:11848558-11848577 None:intergenic 45.0%
AAGCTTGGCTGTCACCAAAT+TGG + chr8_3:11848559-11848578 None:intergenic 45.0%
GAAAGCAGGAAGAGAGGAAT+AGG + chr8_3:11848594-11848613 None:intergenic 45.0%
ACGGACTCTCAGTATAAAGC+TGG - chr8_3:11848620-11848639 Msa1276900:intron 45.0%
AATGGGGACTTGGACATAGT+GGG + chr8_3:11848744-11848763 None:intergenic 45.0%
AAATGGGGACTTGGACATAG+TGG + chr8_3:11848745-11848764 None:intergenic 45.0%
AGCATCCACATCCACTTCTT+AGG + chr8_3:11848821-11848840 None:intergenic 45.0%
! AGTTGAGAAGCAAGGCATTC+TGG + chr8_3:11849069-11849088 None:intergenic 45.0%
!! TGAGTAGCGGATTCAATGGC+TGG + chr8_3:11847745-11847764 None:intergenic 50.0%
! GGTTGAGCTAGAGGTGTGAA+TGG + chr8_3:11847829-11847848 None:intergenic 50.0%
TAACGGTGTGGTTGAGCTAG+AGG + chr8_3:11847838-11847857 None:intergenic 50.0%
! TCGCTGCTGCTTCTTTGACT+TGG + chr8_3:11847951-11847970 None:intergenic 50.0%
CAAAGAAGCAGCAGCGATCA+TGG - chr8_3:11847954-11847973 Msa1276900:intron 50.0%
ACCCTGTCGCACTCTTCAAT+TGG + chr8_3:11847983-11848002 None:intergenic 50.0%
GGTGTCTTTGGTTGGGAGAA+AGG + chr8_3:11848046-11848065 None:intergenic 50.0%
TGAGAGAATGCTTGTGGCGA+CGG + chr8_3:11848067-11848086 None:intergenic 50.0%
GTGAGGACTGAGAGCCATAA+TGG + chr8_3:11848107-11848126 None:intergenic 50.0%
GATCGGTGAAATCGAGAGTG+AGG + chr8_3:11848124-11848143 None:intergenic 50.0%
TCCTCTCTTCCTGCTTTCCA+AGG - chr8_3:11848596-11848615 Msa1276900:intron 50.0%
TCTTCCTGCTTTCCAAGGCA+CGG - chr8_3:11848601-11848620 Msa1276900:intron 50.0%
TATACTGAGAGTCCGTGCCT+TGG + chr8_3:11848616-11848635 None:intergenic 50.0%
CTTGGCCTAAGAAGTGGATG+TGG - chr8_3:11848813-11848832 Msa1276900:three_prime_UTR 50.0%
TTGAATGACTAGGGGAGGCT+AGG - chr8_3:11849274-11849293 Msa1276900:CDS 50.0%
!! TGAGTAGCGGATTCAATGGC+TGG + chr8_3:11847745-11847764 None:intergenic 50.0%
! GGTTGAGCTAGAGGTGTGAA+TGG + chr8_3:11847829-11847848 None:intergenic 50.0%
TAACGGTGTGGTTGAGCTAG+AGG + chr8_3:11847838-11847857 None:intergenic 50.0%
! TCGCTGCTGCTTCTTTGACT+TGG + chr8_3:11847951-11847970 None:intergenic 50.0%
CAAAGAAGCAGCAGCGATCA+TGG - chr8_3:11847954-11847973 Msa1276900:intron 50.0%
ACCCTGTCGCACTCTTCAAT+TGG + chr8_3:11847983-11848002 None:intergenic 50.0%
GGTGTCTTTGGTTGGGAGAA+AGG + chr8_3:11848046-11848065 None:intergenic 50.0%
TGAGAGAATGCTTGTGGCGA+CGG + chr8_3:11848067-11848086 None:intergenic 50.0%
GTGAGGACTGAGAGCCATAA+TGG + chr8_3:11848107-11848126 None:intergenic 50.0%
GATCGGTGAAATCGAGAGTG+AGG + chr8_3:11848124-11848143 None:intergenic 50.0%
TCCTCTCTTCCTGCTTTCCA+AGG - chr8_3:11848596-11848615 Msa1276900:intron 50.0%
TCTTCCTGCTTTCCAAGGCA+CGG - chr8_3:11848601-11848620 Msa1276900:intron 50.0%
TATACTGAGAGTCCGTGCCT+TGG + chr8_3:11848616-11848635 None:intergenic 50.0%
CTTGGCCTAAGAAGTGGATG+TGG - chr8_3:11848813-11848832 Msa1276900:three_prime_UTR 50.0%
TTGAATGACTAGGGGAGGCT+AGG - chr8_3:11849274-11849293 Msa1276900:CDS 50.0%
!! TAGCGGATTCAATGGCTGGC+TGG + chr8_3:11847741-11847760 None:intergenic 55.0%
CACGCCACGAGATTCAGCTT+CGG - chr8_3:11847871-11847890 Msa1276900:intron 55.0%
TGGCGACGGTGTCTTTGGTT+GGG + chr8_3:11848053-11848072 None:intergenic 55.0%
GCCTTGGAAAGCAGGAAGAG+AGG + chr8_3:11848600-11848619 None:intergenic 55.0%
!! TAGCGGATTCAATGGCTGGC+TGG + chr8_3:11847741-11847760 None:intergenic 55.0%
CACGCCACGAGATTCAGCTT+CGG - chr8_3:11847871-11847890 Msa1276900:intron 55.0%
TGGCGACGGTGTCTTTGGTT+GGG + chr8_3:11848053-11848072 None:intergenic 55.0%
GCCTTGGAAAGCAGGAAGAG+AGG + chr8_3:11848600-11848619 None:intergenic 55.0%
! GGATTCAATGGCTGGCTGGC+TGG + chr8_3:11847737-11847756 None:intergenic 60.0%
CGAAGCTGAATCTCGTGGCG+TGG + chr8_3:11847873-11847892 None:intergenic 60.0%
!! CGACAGGGTTAGCTCCCTTC+CGG - chr8_3:11847994-11848013 Msa1276900:intron 60.0%
GTGGCGACGGTGTCTTTGGT+TGG + chr8_3:11848054-11848073 None:intergenic 60.0%
GCTTGTGGCGACGGTGTCTT+TGG + chr8_3:11848058-11848077 None:intergenic 60.0%
GAGTCCGTGCCTTGGAAAGC+AGG + chr8_3:11848608-11848627 None:intergenic 60.0%
! GGATTCAATGGCTGGCTGGC+TGG + chr8_3:11847737-11847756 None:intergenic 60.0%
CGAAGCTGAATCTCGTGGCG+TGG + chr8_3:11847873-11847892 None:intergenic 60.0%
!! CGACAGGGTTAGCTCCCTTC+CGG - chr8_3:11847994-11848013 Msa1276900:intron 60.0%
GTGGCGACGGTGTCTTTGGT+TGG + chr8_3:11848054-11848073 None:intergenic 60.0%
GCTTGTGGCGACGGTGTCTT+TGG + chr8_3:11848058-11848077 None:intergenic 60.0%
GAGTCCGTGCCTTGGAAAGC+AGG + chr8_3:11848608-11848627 None:intergenic 60.0%
! GGCTGGCTGGCTAGCGCTGT+TGG + chr8_3:11847724-11847743 None:intergenic 70.0%
! GGCTGGCTGGCTAGCGCTGT+TGG + chr8_3:11847724-11847743 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 11847703 11849584 11847703 ID=Msa1276900;Name=Msa1276900
chr8_3 mRNA 11847703 11849584 11847703 ID=Msa1276900-mRNA-1;Parent=Msa1276900;Name=Msa1276900-mRNA-1;_AED=0.18;_eAED=0.18;_QI=241|0.5|0.66|0.66|0.5|0.66|3|545|129
chr8_3 exon 11847703 11847783 11847703 ID=Msa1276900-mRNA-1:exon:4806;Parent=Msa1276900-mRNA-1
chr8_3 exon 11848398 11848553 11848398 ID=Msa1276900-mRNA-1:exon:4805;Parent=Msa1276900-mRNA-1
chr8_3 exon 11848646 11849584 11848646 ID=Msa1276900-mRNA-1:exon:4804;Parent=Msa1276900-mRNA-1
chr8_3 five_prime_UTR 11849344 11849584 11849344 ID=Msa1276900-mRNA-1:five_prime_utr;Parent=Msa1276900-mRNA-1
chr8_3 CDS 11848954 11849343 11848954 ID=Msa1276900-mRNA-1:cds;Parent=Msa1276900-mRNA-1
chr8_3 three_prime_UTR 11848646 11848953 11848646 ID=Msa1276900-mRNA-1:three_prime_utr;Parent=Msa1276900-mRNA-1
chr8_3 three_prime_UTR 11848398 11848553 11848398 ID=Msa1276900-mRNA-1:three_prime_utr;Parent=Msa1276900-mRNA-1
chr8_3 three_prime_UTR 11847703 11847783 11847703 ID=Msa1276900-mRNA-1:three_prime_utr;Parent=Msa1276900-mRNA-1
Gene Sequence

>Msa1276900

ATGTCCAAGTCAAAGAAGCAGCAGCGATCATGGATTCCAATTGAAGAGTGCGACAGGGTTAGCTCCCTTCCGGACTCAATTATATGTCATATATTCTCTCCTTTCTCCCAACCAAAGACACCGTCGCCACAAGCATTCTCTCAAAGAGATGGAAACCATACCATTATGGCTCTCAGTCCTCACTCTCGATTTCACCGATCACGAATTCTTCTCACTATTTGCAGCCAGATGTACTCAGTCATGCTATCACGCGACAACACACTGCCAATACTATCACTCCGCCTCAAAAACCCAATGATATCACTGAACTTATTATTGCAGCAATACAAAGACGAACTGAAACCCTTGAACTTGACATGTGAAATTAGCTTCCAACATATTCACTTGTAG
Protein sequence

>Msa1276900

MSKSKKQQRSWIPIEECDRVSSLPDSIICHIFSPFSQPKTPSPQAFSQRDGNHTIMALSPHSRFHRSRILLTICSQMYSVMLSRDNTLPILSLRLKNPMISLNLLLQQYKDELKPLNLTCEISFQHIHL*