AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1176330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1176330 MtrunA17_Chr8g0346501 100.000 106 0 0 1 106 1 106 3.26e-77 222
Msa1176330 MtrunA17_Chr3g0127211 67.619 105 34 0 1 105 1 105 1.43e-50 155
Msa1176330 MtrunA17_Chr1g0188931 63.725 102 37 0 1 102 1 102 1.13e-47 147
Msa1176330 MtrunA17_Chr5g0436971 50.505 99 49 0 8 106 12 110 1.38e-33 113
Msa1176330 MtrunA17_Chr3g0113291 52.128 94 45 0 8 101 35 128 1.86e-33 113
Msa1176330 MtrunA17_Chr4g0046851 50.000 100 49 1 3 102 8 106 8.28e-31 105
Msa1176330 MtrunA17_Chr7g0242231 48.454 97 50 0 10 106 14 110 4.40e-30 103
Msa1176330 MtrunA17_Chr2g0316921 48.421 95 49 0 8 102 12 106 7.52e-29 100
Msa1176330 MtrunA17_Chr8g0360291 44.211 95 53 0 8 102 12 106 1.91e-26 95.1
Msa1176330 MtrunA17_Chr1g0188951 64.516 62 22 0 44 105 1 62 2.60e-22 82.4
Msa1176330 MtrunA17_Chr2g0316891 50.794 63 31 0 40 102 42 104 1.40e-18 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1176330 AT4G27745.2 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1176330 AT4G27745.3 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1176330 AT4G27745.1 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1176330 AT4G27745.4 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1176330 AT3G11230.1 50.505 99 49 0 8 106 12 110 1.23e-34 115
Msa1176330 AT3G11230.2 50.505 99 49 0 8 106 45 143 4.47e-34 115
Msa1176330 AT3G11230.4 52.174 92 44 0 8 99 12 103 1.76e-33 112
Msa1176330 AT4G27740.1 51.064 94 46 0 9 102 9 102 2.71e-33 112
Msa1176330 AT3G55890.4 52.475 101 47 1 1 101 6 105 3.21e-33 112
Msa1176330 AT3G55890.1 52.475 101 47 1 1 101 6 105 3.21e-33 112
Msa1176330 AT3G11230.3 52.747 91 43 0 8 98 45 135 6.02e-33 112
Msa1176330 AT5G53940.1 50.526 95 47 0 8 102 12 106 2.25e-32 110
Msa1176330 AT2G40110.1 49.495 99 50 0 8 106 12 110 3.91e-32 109
Msa1176330 AT3G55890.3 52.577 97 45 1 1 97 6 101 4.46e-32 108
Msa1176330 AT3G55890.2 52.577 97 45 1 1 97 6 101 4.46e-32 108
Msa1176330 AT3G08990.2 50.000 102 50 1 1 102 6 106 9.04e-32 108
Msa1176330 AT3G08990.1 50.000 102 50 1 1 102 6 106 9.04e-32 108
Msa1176330 AT3G08990.3 50.000 102 50 1 1 102 6 106 9.04e-32 108
Msa1176330 AT2G40110.2 50.000 92 46 0 8 99 12 103 8.94e-31 105
Msa1176330 AT2G40110.4 45.205 73 40 0 8 80 12 84 1.29e-21 82.0
Msa1176330 AT2G40110.3 45.205 73 40 0 8 80 12 84 1.29e-21 82.0

Find 33 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTTTCCTCATAAGCTTTA+AGG 0.102549 8_1:+8321210 None:intergenic
TGAGAAGTTTAAGATTGTTT+TGG 0.258269 8_1:-8321159 Msa1176330:CDS
AAAGTTCACCTGAAAATCTT+TGG 0.258418 8_1:+8322137 None:intergenic
ATAAGCTGTCTATCTTCTTT+AGG 0.260634 8_1:+8321299 None:intergenic
TTTAACATTAATTGTTCATT+GGG 0.318516 8_1:-8321111 Msa1176330:three_prime_UTR
ATCATTAATGGCCGATTTGA+TGG 0.329137 8_1:-8322223 Msa1176330:exon
ACCATTCAACAATTAACATT+AGG 0.330744 8_1:+8320948 None:intergenic
CCATTCAACAATTAACATTA+GGG 0.341166 8_1:+8320949 None:intergenic
CATGGAAAGCAACAAGGTTC+TGG 0.376925 8_1:+8322172 None:intergenic
GGAGCTAAGGTGGCAGTGTT+AGG 0.379271 8_1:-8322253 Msa1176330:five_prime_UTR
CCCTAATGTTAATTGTTGAA+TGG 0.397010 8_1:-8320949 Msa1176330:three_prime_UTR
TTTGTAAATGCTGCTTGATC+AGG 0.404842 8_1:-8321044 Msa1176330:three_prime_UTR
GAAATACCTTAAAGCTTATG+AGG 0.433394 8_1:-8321216 Msa1176330:CDS
CAACTCTGACATGAAAAGAT+TGG 0.449933 8_1:-8322397 Msa1176330:five_prime_UTR
CAACAAACCTTCTCTGTACA+AGG 0.478519 8_1:+8320868 None:intergenic
GGCCGATTTGATGGGATCAC+GGG 0.488237 8_1:-8322214 Msa1176330:CDS
TGCCCGTGATCCCATCAAAT+CGG 0.500160 8_1:+8322212 None:intergenic
TTATTAGCCTTGTACAGAGA+AGG 0.526516 8_1:-8320875 Msa1176330:three_prime_UTR
TCATTAATGGCCGATTTGAT+GGG 0.534012 8_1:-8322222 Msa1176330:exon
TGGCCGATTTGATGGGATCA+CGG 0.537450 8_1:-8322215 Msa1176330:CDS
TTGTAAATGCTGCTTGATCA+GGG 0.538347 8_1:-8321043 Msa1176330:three_prime_UTR
TATGTTTAACAGGCAAGCAA+TGG 0.540111 8_1:-8321365 Msa1176330:intron
CTTTGGAAACAATATCATCA+TGG 0.545782 8_1:+8322154 None:intergenic
TCTGATTGTGGAGAGGAACT+TGG 0.548681 8_1:-8321242 Msa1176330:CDS
GAAGATAGACAGCTTATGAC+TGG 0.563935 8_1:-8321293 Msa1176330:CDS
GAAACACAGAAGTATAAAGA+AGG 0.567035 8_1:-8321194 Msa1176330:CDS
TTATGTATGAGTAGGAGCTA+AGG 0.582837 8_1:-8322266 Msa1176330:intron
TTATACTAATGTTGTACATG+TGG 0.589916 8_1:+8321083 None:intergenic
ATTGTGGAGAGGAACTTGGT+TGG 0.615032 8_1:-8321238 Msa1176330:CDS
TGTATGAGTAGGAGCTAAGG+TGG 0.621962 8_1:-8322263 Msa1176330:intron
TATCATCATGGAAAGCAACA+AGG 0.636612 8_1:+8322166 None:intergenic
TTAACATTAATTGTTCATTG+GGG 0.648404 8_1:-8321110 Msa1176330:three_prime_UTR
CATTCAACAATTAACATTAG+GGG 0.686208 8_1:+8320950 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTGTTGAATATTAAT+TGG - chr8_1:8321812-8321831 Msa1176330:intron 10.0%
!!! TTAATTTGTTGAATATTAAT+TGG - chr8_1:8321812-8321831 Msa1176330:intron 10.0%
!! TATATTATTTATGTATGAGT+AGG - chr8_1:8320989-8321008 Msa1176330:three_prime_UTR 15.0%
!! TTAGGAAAAAAAATCATTAA+TGG - chr8_1:8321028-8321047 Msa1176330:three_prime_UTR 15.0%
!!! AATTTGTAGTAAGATTTTTT+CGG + chr8_1:8321721-8321740 None:intergenic 15.0%
!!! TTTTAACATTAATTGTTCAT+TGG - chr8_1:8322151-8322170 Msa1176330:CDS 15.0%
!! TTTAACATTAATTGTTCATT+GGG - chr8_1:8322152-8322171 Msa1176330:CDS 15.0%
!! TATATTATTTATGTATGAGT+AGG - chr8_1:8320989-8321008 Msa1176330:three_prime_UTR 15.0%
!! TTAGGAAAAAAAATCATTAA+TGG - chr8_1:8321028-8321047 Msa1176330:three_prime_UTR 15.0%
!!! AATTTGTAGTAAGATTTTTT+CGG + chr8_1:8321721-8321740 None:intergenic 15.0%
!!! TTTTAACATTAATTGTTCAT+TGG - chr8_1:8322151-8322170 Msa1176330:CDS 15.0%
!! TTTAACATTAATTGTTCATT+GGG - chr8_1:8322152-8322171 Msa1176330:CDS 15.0%
!! AGTATATAAAAGAAAAAGCT+TGG + chr8_1:8320932-8320951 None:intergenic 20.0%
!! ATAGATTTCTATGTTGAAAA+TGG - chr8_1:8321186-8321205 Msa1176330:CDS 20.0%
!! GAAAATTTGTCTAAATCAAA+TGG - chr8_1:8321281-8321300 Msa1176330:CDS 20.0%
!!! ATTTGTAGTAAGATTTTTTC+GGG + chr8_1:8321720-8321739 None:intergenic 20.0%
!! TTAACATTAATTGTTCATTG+GGG - chr8_1:8322153-8322172 Msa1176330:CDS 20.0%
!! AGTATATAAAAGAAAAAGCT+TGG + chr8_1:8320932-8320951 None:intergenic 20.0%
!! ATAGATTTCTATGTTGAAAA+TGG - chr8_1:8321186-8321205 Msa1176330:CDS 20.0%
!! GAAAATTTGTCTAAATCAAA+TGG - chr8_1:8321281-8321300 Msa1176330:CDS 20.0%
!!! ATTTGTAGTAAGATTTTTTC+GGG + chr8_1:8321720-8321739 None:intergenic 20.0%
!! TTAACATTAATTGTTCATTG+GGG - chr8_1:8322153-8322172 Msa1176330:CDS 20.0%
!! TATTGTTTCCAAAGATTTTC+AGG - chr8_1:8321118-8321137 Msa1176330:three_prime_UTR 25.0%
! TATTAGATGATCCAATTTCA+TGG + chr8_1:8321217-8321236 None:intergenic 25.0%
!! AATTTTCTCTCTAAATCCAA+CGG + chr8_1:8321268-8321287 None:intergenic 25.0%
!!! TAGAAGATATTTTTTGGGTT+TGG - chr8_1:8321366-8321385 Msa1176330:intron 25.0%
!! TTCATCATGGTTTAAAGTTT+GGG + chr8_1:8321472-8321491 None:intergenic 25.0%
!! TTTCATCATGGTTTAAAGTT+TGG + chr8_1:8321473-8321492 None:intergenic 25.0%
! AGACAAGTGAAATTAATTTC+AGG + chr8_1:8321511-8321530 None:intergenic 25.0%
! ATTAATTTCACTTGTCTTGA+TGG - chr8_1:8321514-8321533 Msa1176330:intron 25.0%
! TTCAATTAAGGTTGAATCAA+TGG - chr8_1:8321588-8321607 Msa1176330:intron 25.0%
!!! ACCAATGATTGTTTTAGTTA+GGG + chr8_1:8321631-8321650 None:intergenic 25.0%
!!! AACCAATGATTGTTTTAGTT+AGG + chr8_1:8321632-8321651 None:intergenic 25.0%
! TTGAATATTAATTGGACAAG+AGG - chr8_1:8321820-8321839 Msa1176330:intron 25.0%
! TGAATATTAATTGGACAAGA+GGG - chr8_1:8321821-8321840 Msa1176330:intron 25.0%
!! TAACCTCAAAAGATTTTCAA+AGG - chr8_1:8321874-8321893 Msa1176330:intron 25.0%
!!! TAACCTTTGAAAATCTTTTG+AGG + chr8_1:8321880-8321899 None:intergenic 25.0%
! TTTCAAAGGTTATGTTTAAC+AGG - chr8_1:8321888-8321907 Msa1176330:intron 25.0%
!!! TGAGAAGTTTAAGATTGTTT+TGG - chr8_1:8322104-8322123 Msa1176330:intron 25.0%
!!! GAGAAGTTTAAGATTGTTTT+GGG - chr8_1:8322105-8322124 Msa1176330:intron 25.0%
!!! TTTAAGATTGTTTTGGGAAA+TGG - chr8_1:8322111-8322130 Msa1176330:intron 25.0%
!!! TTAAGATTGTTTTGGGAAAT+GGG - chr8_1:8322112-8322131 Msa1176330:intron 25.0%
! TTATACTAATGTTGTACATG+TGG + chr8_1:8322183-8322202 None:intergenic 25.0%
! CATTCAACAATTAACATTAG+GGG + chr8_1:8322316-8322335 None:intergenic 25.0%
! CCATTCAACAATTAACATTA+GGG + chr8_1:8322317-8322336 None:intergenic 25.0%
! ACCATTCAACAATTAACATT+AGG + chr8_1:8322318-8322337 None:intergenic 25.0%
!! TCTTTTGTACAATATGAACA+AGG + chr8_1:8322341-8322360 None:intergenic 25.0%
!! TATTGTTTCCAAAGATTTTC+AGG - chr8_1:8321118-8321137 Msa1176330:three_prime_UTR 25.0%
! TATTAGATGATCCAATTTCA+TGG + chr8_1:8321217-8321236 None:intergenic 25.0%
!! AATTTTCTCTCTAAATCCAA+CGG + chr8_1:8321268-8321287 None:intergenic 25.0%
!!! TAGAAGATATTTTTTGGGTT+TGG - chr8_1:8321366-8321385 Msa1176330:intron 25.0%
!! TTCATCATGGTTTAAAGTTT+GGG + chr8_1:8321472-8321491 None:intergenic 25.0%
!! TTTCATCATGGTTTAAAGTT+TGG + chr8_1:8321473-8321492 None:intergenic 25.0%
! AGACAAGTGAAATTAATTTC+AGG + chr8_1:8321511-8321530 None:intergenic 25.0%
! ATTAATTTCACTTGTCTTGA+TGG - chr8_1:8321514-8321533 Msa1176330:intron 25.0%
! TTCAATTAAGGTTGAATCAA+TGG - chr8_1:8321588-8321607 Msa1176330:intron 25.0%
!!! ACCAATGATTGTTTTAGTTA+GGG + chr8_1:8321631-8321650 None:intergenic 25.0%
!!! AACCAATGATTGTTTTAGTT+AGG + chr8_1:8321632-8321651 None:intergenic 25.0%
! TTGAATATTAATTGGACAAG+AGG - chr8_1:8321820-8321839 Msa1176330:intron 25.0%
! TGAATATTAATTGGACAAGA+GGG - chr8_1:8321821-8321840 Msa1176330:intron 25.0%
!! TAACCTCAAAAGATTTTCAA+AGG - chr8_1:8321874-8321893 Msa1176330:intron 25.0%
!!! TAACCTTTGAAAATCTTTTG+AGG + chr8_1:8321880-8321899 None:intergenic 25.0%
! TTTCAAAGGTTATGTTTAAC+AGG - chr8_1:8321888-8321907 Msa1176330:intron 25.0%
!!! TGAGAAGTTTAAGATTGTTT+TGG - chr8_1:8322104-8322123 Msa1176330:intron 25.0%
!!! GAGAAGTTTAAGATTGTTTT+GGG - chr8_1:8322105-8322124 Msa1176330:intron 25.0%
!!! TTTAAGATTGTTTTGGGAAA+TGG - chr8_1:8322111-8322130 Msa1176330:intron 25.0%
!!! TTAAGATTGTTTTGGGAAAT+GGG - chr8_1:8322112-8322131 Msa1176330:intron 25.0%
! TTATACTAATGTTGTACATG+TGG + chr8_1:8322183-8322202 None:intergenic 25.0%
! CATTCAACAATTAACATTAG+GGG + chr8_1:8322316-8322335 None:intergenic 25.0%
! CCATTCAACAATTAACATTA+GGG + chr8_1:8322317-8322336 None:intergenic 25.0%
! ACCATTCAACAATTAACATT+AGG + chr8_1:8322318-8322337 None:intergenic 25.0%
!! TCTTTTGTACAATATGAACA+AGG + chr8_1:8322341-8322360 None:intergenic 25.0%
! CTTTGGAAACAATATCATCA+TGG + chr8_1:8321112-8321131 None:intergenic 30.0%
AAAGTTCACCTGAAAATCTT+TGG + chr8_1:8321129-8321148 None:intergenic 30.0%
AAATGGTTCATCCATGAAAT+TGG - chr8_1:8321203-8321222 Msa1176330:CDS 30.0%
AATTGGATCATCTAATAGTG+CGG - chr8_1:8321220-8321239 Msa1176330:CDS 30.0%
! AAAATATCTTCTAGTGGGTA+TGG + chr8_1:8321359-8321378 None:intergenic 30.0%
!!! ACCCACTAGAAGATATTTTT+TGG - chr8_1:8321360-8321379 Msa1176330:intron 30.0%
CCCAAAAAATATCTTCTAGT+GGG + chr8_1:8321364-8321383 None:intergenic 30.0%
!!! CCCACTAGAAGATATTTTTT+GGG - chr8_1:8321361-8321380 Msa1176330:intron 30.0%
ACCCAAAAAATATCTTCTAG+TGG + chr8_1:8321365-8321384 None:intergenic 30.0%
TGTTTGAAAAGCTTTCATCA+TGG + chr8_1:8321485-8321504 None:intergenic 30.0%
TCCCTAACTAAAACAATCAT+TGG - chr8_1:8321627-8321646 Msa1176330:intron 30.0%
TTTGTGACCTAACTCAAATT+AGG - chr8_1:8321665-8321684 Msa1176330:intron 30.0%
! AAATGGACCTAATTTGAGTT+AGG + chr8_1:8321675-8321694 None:intergenic 30.0%
! TCATGCCATGAACATATTTT+TGG - chr8_1:8321939-8321958 Msa1176330:intron 30.0%
! CATGCCATGAACATATTTTT+GGG - chr8_1:8321940-8321959 Msa1176330:intron 30.0%
ATAAGCTGTCTATCTTCTTT+AGG + chr8_1:8321967-8321986 None:intergenic 30.0%
!!! GATGTTTTTTGTTCTGATTG+TGG - chr8_1:8322009-8322028 Msa1176330:intron 30.0%
GAAATACCTTAAAGCTTATG+AGG - chr8_1:8322047-8322066 Msa1176330:intron 30.0%
! GAAACACAGAAGTATAAAGA+AGG - chr8_1:8322069-8322088 Msa1176330:intron 30.0%
CCCTAATGTTAATTGTTGAA+TGG - chr8_1:8322314-8322333 Msa1176330:intron 30.0%
! CTTTGGAAACAATATCATCA+TGG + chr8_1:8321112-8321131 None:intergenic 30.0%
AAAGTTCACCTGAAAATCTT+TGG + chr8_1:8321129-8321148 None:intergenic 30.0%
AAATGGTTCATCCATGAAAT+TGG - chr8_1:8321203-8321222 Msa1176330:CDS 30.0%
AATTGGATCATCTAATAGTG+CGG - chr8_1:8321220-8321239 Msa1176330:CDS 30.0%
! AAAATATCTTCTAGTGGGTA+TGG + chr8_1:8321359-8321378 None:intergenic 30.0%
!!! ACCCACTAGAAGATATTTTT+TGG - chr8_1:8321360-8321379 Msa1176330:intron 30.0%
CCCAAAAAATATCTTCTAGT+GGG + chr8_1:8321364-8321383 None:intergenic 30.0%
!!! CCCACTAGAAGATATTTTTT+GGG - chr8_1:8321361-8321380 Msa1176330:intron 30.0%
ACCCAAAAAATATCTTCTAG+TGG + chr8_1:8321365-8321384 None:intergenic 30.0%
TGTTTGAAAAGCTTTCATCA+TGG + chr8_1:8321485-8321504 None:intergenic 30.0%
TCCCTAACTAAAACAATCAT+TGG - chr8_1:8321627-8321646 Msa1176330:intron 30.0%
TTTGTGACCTAACTCAAATT+AGG - chr8_1:8321665-8321684 Msa1176330:intron 30.0%
! AAATGGACCTAATTTGAGTT+AGG + chr8_1:8321675-8321694 None:intergenic 30.0%
! TCATGCCATGAACATATTTT+TGG - chr8_1:8321939-8321958 Msa1176330:intron 30.0%
! CATGCCATGAACATATTTTT+GGG - chr8_1:8321940-8321959 Msa1176330:intron 30.0%
ATAAGCTGTCTATCTTCTTT+AGG + chr8_1:8321967-8321986 None:intergenic 30.0%
!!! GATGTTTTTTGTTCTGATTG+TGG - chr8_1:8322009-8322028 Msa1176330:intron 30.0%
GAAATACCTTAAAGCTTATG+AGG - chr8_1:8322047-8322066 Msa1176330:intron 30.0%
! GAAACACAGAAGTATAAAGA+AGG - chr8_1:8322069-8322088 Msa1176330:intron 30.0%
CCCTAATGTTAATTGTTGAA+TGG - chr8_1:8322314-8322333 Msa1176330:intron 30.0%
! CAATCTTTTCATGTCAGAGT+TGG + chr8_1:8320868-8320887 None:intergenic 35.0%
CAACTCTGACATGAAAAGAT+TGG - chr8_1:8320866-8320885 Msa1176330:three_prime_UTR 35.0%
TTATGTATGAGTAGGAGCTA+AGG - chr8_1:8320997-8321016 Msa1176330:three_prime_UTR 35.0%
!! ATCATTAATGGCCGATTTGA+TGG - chr8_1:8321040-8321059 Msa1176330:three_prime_UTR 35.0%
!! TCATTAATGGCCGATTTGAT+GGG - chr8_1:8321041-8321060 Msa1176330:three_prime_UTR 35.0%
TATCATCATGGAAAGCAACA+AGG + chr8_1:8321100-8321119 None:intergenic 35.0%
!! TTCAAAGTCTTGTTCTCTAC+TGG + chr8_1:8321399-8321418 None:intergenic 35.0%
TGGCTAATGAGCTTCAATTA+AGG - chr8_1:8321576-8321595 Msa1176330:intron 35.0%
TCAATGGAAGAAACTGAGTT+CGG - chr8_1:8321604-8321623 Msa1176330:intron 35.0%
ATATTAATTGGACAAGAGGG+TGG - chr8_1:8321824-8321843 Msa1176330:intron 35.0%
TATGTTTAACAGGCAAGCAA+TGG - chr8_1:8321898-8321917 Msa1176330:intron 35.0%
AGGTCCCAAAAATATGTTCA+TGG + chr8_1:8321947-8321966 None:intergenic 35.0%
! TTTTTGTTCTGATTGTGGAG+AGG - chr8_1:8322014-8322033 Msa1176330:intron 35.0%
GTGTTTCCTCATAAGCTTTA+AGG + chr8_1:8322056-8322075 None:intergenic 35.0%
! TTTGTAAATGCTGCTTGATC+AGG - chr8_1:8322219-8322238 Msa1176330:CDS 35.0%
! TTGTAAATGCTGCTTGATCA+GGG - chr8_1:8322220-8322239 Msa1176330:exon 35.0%
!! TTATTAGCCTTGTACAGAGA+AGG - chr8_1:8322388-8322407 Msa1176330:five_prime_UTR 35.0%
! CAATCTTTTCATGTCAGAGT+TGG + chr8_1:8320868-8320887 None:intergenic 35.0%
CAACTCTGACATGAAAAGAT+TGG - chr8_1:8320866-8320885 Msa1176330:three_prime_UTR 35.0%
TTATGTATGAGTAGGAGCTA+AGG - chr8_1:8320997-8321016 Msa1176330:three_prime_UTR 35.0%
!! ATCATTAATGGCCGATTTGA+TGG - chr8_1:8321040-8321059 Msa1176330:three_prime_UTR 35.0%
!! TCATTAATGGCCGATTTGAT+GGG - chr8_1:8321041-8321060 Msa1176330:three_prime_UTR 35.0%
TATCATCATGGAAAGCAACA+AGG + chr8_1:8321100-8321119 None:intergenic 35.0%
!! TTCAAAGTCTTGTTCTCTAC+TGG + chr8_1:8321399-8321418 None:intergenic 35.0%
TGGCTAATGAGCTTCAATTA+AGG - chr8_1:8321576-8321595 Msa1176330:intron 35.0%
TCAATGGAAGAAACTGAGTT+CGG - chr8_1:8321604-8321623 Msa1176330:intron 35.0%
ATATTAATTGGACAAGAGGG+TGG - chr8_1:8321824-8321843 Msa1176330:intron 35.0%
TATGTTTAACAGGCAAGCAA+TGG - chr8_1:8321898-8321917 Msa1176330:intron 35.0%
AGGTCCCAAAAATATGTTCA+TGG + chr8_1:8321947-8321966 None:intergenic 35.0%
! TTTTTGTTCTGATTGTGGAG+AGG - chr8_1:8322014-8322033 Msa1176330:intron 35.0%
GTGTTTCCTCATAAGCTTTA+AGG + chr8_1:8322056-8322075 None:intergenic 35.0%
! TTTGTAAATGCTGCTTGATC+AGG - chr8_1:8322219-8322238 Msa1176330:CDS 35.0%
! TTGTAAATGCTGCTTGATCA+GGG - chr8_1:8322220-8322239 Msa1176330:exon 35.0%
!! TTATTAGCCTTGTACAGAGA+AGG - chr8_1:8322388-8322407 Msa1176330:five_prime_UTR 35.0%
TCAAATGGTTCCTACCACTA+TGG - chr8_1:8321296-8321315 Msa1176330:CDS 40.0%
TGGGTATGGATGAACGAAAT+TGG + chr8_1:8321345-8321364 None:intergenic 40.0%
TAGGTCCATTTCCCATATTC+CGG - chr8_1:8321684-8321703 Msa1176330:intron 40.0%
!! GTTTTAACCCTCCGGAATAT+GGG + chr8_1:8321698-8321717 None:intergenic 40.0%
!!! TTTTTCGGGTTTTAACCCTC+CGG + chr8_1:8321706-8321725 None:intergenic 40.0%
GAAGATAGACAGCTTATGAC+TGG - chr8_1:8321970-8321989 Msa1176330:intron 40.0%
CAACAAACCTTCTCTGTACA+AGG + chr8_1:8322398-8322417 None:intergenic 40.0%
TCAAATGGTTCCTACCACTA+TGG - chr8_1:8321296-8321315 Msa1176330:CDS 40.0%
TGGGTATGGATGAACGAAAT+TGG + chr8_1:8321345-8321364 None:intergenic 40.0%
TAGGTCCATTTCCCATATTC+CGG - chr8_1:8321684-8321703 Msa1176330:intron 40.0%
!! GTTTTAACCCTCCGGAATAT+GGG + chr8_1:8321698-8321717 None:intergenic 40.0%
!!! TTTTTCGGGTTTTAACCCTC+CGG + chr8_1:8321706-8321725 None:intergenic 40.0%
GAAGATAGACAGCTTATGAC+TGG - chr8_1:8321970-8321989 Msa1176330:intron 40.0%
CAACAAACCTTCTCTGTACA+AGG + chr8_1:8322398-8322417 None:intergenic 40.0%
TGTATGAGTAGGAGCTAAGG+TGG - chr8_1:8321000-8321019 Msa1176330:three_prime_UTR 45.0%
CATGGAAAGCAACAAGGTTC+TGG + chr8_1:8321094-8321113 None:intergenic 45.0%
TAGTGCGAAAGACACCATAG+TGG + chr8_1:8321313-8321332 None:intergenic 45.0%
TATGGTGTCTTTCGCACTAG+TGG - chr8_1:8321314-8321333 Msa1176330:CDS 45.0%
TCCATTTCCCATATTCCGGA+GGG - chr8_1:8321688-8321707 Msa1176330:intron 45.0%
!! GGTTTTAACCCTCCGGAATA+TGG + chr8_1:8321699-8321718 None:intergenic 45.0%
TAATTGGACAAGAGGGTGGT+TGG - chr8_1:8321828-8321847 Msa1176330:intron 45.0%
TCTGATTGTGGAGAGGAACT+TGG - chr8_1:8322021-8322040 Msa1176330:intron 45.0%
ATTGTGGAGAGGAACTTGGT+TGG - chr8_1:8322025-8322044 Msa1176330:intron 45.0%
TGTATGAGTAGGAGCTAAGG+TGG - chr8_1:8321000-8321019 Msa1176330:three_prime_UTR 45.0%
CATGGAAAGCAACAAGGTTC+TGG + chr8_1:8321094-8321113 None:intergenic 45.0%
TAGTGCGAAAGACACCATAG+TGG + chr8_1:8321313-8321332 None:intergenic 45.0%
TATGGTGTCTTTCGCACTAG+TGG - chr8_1:8321314-8321333 Msa1176330:CDS 45.0%
TCCATTTCCCATATTCCGGA+GGG - chr8_1:8321688-8321707 Msa1176330:intron 45.0%
!! GGTTTTAACCCTCCGGAATA+TGG + chr8_1:8321699-8321718 None:intergenic 45.0%
TAATTGGACAAGAGGGTGGT+TGG - chr8_1:8321828-8321847 Msa1176330:intron 45.0%
TCTGATTGTGGAGAGGAACT+TGG - chr8_1:8322021-8322040 Msa1176330:intron 45.0%
ATTGTGGAGAGGAACTTGGT+TGG - chr8_1:8322025-8322044 Msa1176330:intron 45.0%
!! AAATCATATATTATAAATTA+TGG - chr8_1:8321556-8321575 Msa1176330:intron 5.0%
!! AAATCATATATTATAAATTA+TGG - chr8_1:8321556-8321575 Msa1176330:intron 5.0%
!! TGGCCGATTTGATGGGATCA+CGG - chr8_1:8321048-8321067 Msa1176330:three_prime_UTR 50.0%
TGCCCGTGATCCCATCAAAT+CGG + chr8_1:8321054-8321073 None:intergenic 50.0%
AGTGCGGAATGATGCTGCAA+CGG - chr8_1:8321236-8321255 Msa1176330:CDS 50.0%
GCGAAAGACACCATAGTGGT+AGG + chr8_1:8321309-8321328 None:intergenic 50.0%
GTCCATTTCCCATATTCCGG+AGG - chr8_1:8321687-8321706 Msa1176330:intron 50.0%
ACCCTCCGGAATATGGGAAA+TGG + chr8_1:8321692-8321711 None:intergenic 50.0%
!! TGGCCGATTTGATGGGATCA+CGG - chr8_1:8321048-8321067 Msa1176330:three_prime_UTR 50.0%
TGCCCGTGATCCCATCAAAT+CGG + chr8_1:8321054-8321073 None:intergenic 50.0%
AGTGCGGAATGATGCTGCAA+CGG - chr8_1:8321236-8321255 Msa1176330:CDS 50.0%
GCGAAAGACACCATAGTGGT+AGG + chr8_1:8321309-8321328 None:intergenic 50.0%
GTCCATTTCCCATATTCCGG+AGG - chr8_1:8321687-8321706 Msa1176330:intron 50.0%
ACCCTCCGGAATATGGGAAA+TGG + chr8_1:8321692-8321711 None:intergenic 50.0%
GGAGCTAAGGTGGCAGTGTT+AGG - chr8_1:8321010-8321029 Msa1176330:three_prime_UTR 55.0%
!! GGCCGATTTGATGGGATCAC+GGG - chr8_1:8321049-8321068 Msa1176330:three_prime_UTR 55.0%
GCTGCAACGGTAGAAACCGT+TGG - chr8_1:8321249-8321268 Msa1176330:CDS 55.0%
GGAGCTAAGGTGGCAGTGTT+AGG - chr8_1:8321010-8321029 Msa1176330:three_prime_UTR 55.0%
!! GGCCGATTTGATGGGATCAC+GGG - chr8_1:8321049-8321068 Msa1176330:three_prime_UTR 55.0%
GCTGCAACGGTAGAAACCGT+TGG - chr8_1:8321249-8321268 Msa1176330:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 8320861 8322424 8320861 ID=Msa1176330;Name=Msa1176330
chr8_1 mRNA 8320861 8322424 8320861 ID=Msa1176330-mRNA-1;Parent=Msa1176330;Name=Msa1176330-mRNA-1;_AED=0.07;_eAED=0.07;_QI=88|1|1|1|0.5|0.33|3|287|106
chr8_1 exon 8320861 8321375 8320861 ID=Msa1176330-mRNA-1:exon:2877;Parent=Msa1176330-mRNA-1
chr8_1 exon 8322146 8322274 8322146 ID=Msa1176330-mRNA-1:exon:2876;Parent=Msa1176330-mRNA-1
chr8_1 exon 8322373 8322424 8322373 ID=Msa1176330-mRNA-1:exon:2875;Parent=Msa1176330-mRNA-1
chr8_1 five_prime_UTR 8322373 8322424 8322373 ID=Msa1176330-mRNA-1:five_prime_utr;Parent=Msa1176330-mRNA-1
chr8_1 five_prime_UTR 8322239 8322274 8322239 ID=Msa1176330-mRNA-1:five_prime_utr;Parent=Msa1176330-mRNA-1
chr8_1 CDS 8322146 8322238 8322146 ID=Msa1176330-mRNA-1:cds;Parent=Msa1176330-mRNA-1
chr8_1 CDS 8321148 8321375 8321148 ID=Msa1176330-mRNA-1:cds;Parent=Msa1176330-mRNA-1
chr8_1 three_prime_UTR 8320861 8321147 8320861 ID=Msa1176330-mRNA-1:three_prime_utr;Parent=Msa1176330-mRNA-1
Gene Sequence

>Msa1176330

ATGGCCGATTTGATGGGATCACGGGCATACTGCTGCTTCAAGTGCCAGAACCTTGTTGCTTTCCATGATGATATTGTTTCCAAAGATTTTCAGGCAAGCAATGGAAGAGCTTTTCTGTTTTCTCATGCCATGAACATATTTTTGGGACCTAAAGAAGATAGACAGCTTATGACTGGTCTTCACACAGTTGCTGATGTTTTTTGTTCTGATTGTGGAGAGGAACTTGGTTGGAAATACCTTAAAGCTTATGAGGAAACACAGAAGTATAAAGAAGGAAAATGTGTGCTTGAGAAGTTTAAGATTGTTTTGGGAAATGGGTAG
Protein sequence

>Msa1176330

MADLMGSRAYCCFKCQNLVAFHDDIVSKDFQASNGRAFLFSHAMNIFLGPKEDRQLMTGLHTVADVFCSDCGEELGWKYLKAYEETQKYKEGKCVLEKFKIVLGNG*