Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1176750 | Msa1176760 | 0.904082 | 1.779080e-79 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCGATCAGAAGGCATTAC+AGG | 0.325774 | 8_1:-8937550 | Msa1176750:CDS |
| AGGCTTTATCAGGGTGGAAT+TGG | 0.379642 | 8_1:-8937498 | Msa1176750:intron |
| ACTCTTAAACACTCTTAGAT+TGG | 0.421253 | 8_1:-8937441 | None:intergenic |
| ATTGTGGTCTGCAGCGTGTA+AGG | 0.428052 | 8_1:-8937603 | Msa1176750:CDS |
| GCCTTCATAACTAGCATCTC+TGG | 0.458800 | 8_1:+8937475 | None:intergenic |
| TTGAACAAACGTGACTCAAC+AGG | 0.466439 | 8_1:-8937632 | Msa1176750:CDS |
| GCATTACAGGATACGAGTGC+TGG | 0.506841 | 8_1:-8937537 | Msa1176750:CDS |
| GCCAGAGATGCTAGTTATGA+AGG | 0.512673 | 8_1:-8937476 | Msa1176750:CDS |
| TAGTTATGAAGGCGAGGCTA+TGG | 0.534721 | 8_1:-8937465 | Msa1176750:CDS |
| CATTACAGGATACGAGTGCT+GGG | 0.608677 | 8_1:-8937536 | Msa1176750:CDS |
| ACTCAACAGGTAGAATATTG+TGG | 0.611896 | 8_1:-8937619 | Msa1176750:CDS |
| AGATGCTAGTTATGAAGGCG+AGG | 0.642002 | 8_1:-8937471 | Msa1176750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTTGTGAAGGCTTTATC+AGG | - | chr8_1:8937578-8937597 | Msa1176750:CDS | 30.0% |
| !! | ATTTTGTGAAGGCTTTATCA+GGG | - | chr8_1:8937579-8937598 | Msa1176750:CDS | 30.0% |
| ACTCAACAGGTAGAATATTG+TGG | - | chr8_1:8937467-8937486 | Msa1176750:CDS | 35.0% | |
| !!! | CGAGTTTTTTTTCGATCAGA+AGG | - | chr8_1:8937527-8937546 | Msa1176750:intron | 35.0% |
| ! | CTGGGAATGTAATTTTGTGA+AGG | - | chr8_1:8937568-8937587 | Msa1176750:CDS | 35.0% |
| TTGAACAAACGTGACTCAAC+AGG | - | chr8_1:8937454-8937473 | Msa1176750:CDS | 40.0% | |
| !! | TTTCGATCAGAAGGCATTAC+AGG | - | chr8_1:8937536-8937555 | Msa1176750:CDS | 40.0% |
| CATTACAGGATACGAGTGCT+GGG | - | chr8_1:8937550-8937569 | Msa1176750:CDS | 45.0% | |
| ! | TTGTGAAGGCTTTATCAGGG+TGG | - | chr8_1:8937582-8937601 | Msa1176750:CDS | 45.0% |
| ! | AGGCTTTATCAGGGTGGAAT+TGG | - | chr8_1:8937588-8937607 | Msa1176750:CDS | 45.0% |
| GCCAGAGATGCTAGTTATGA+AGG | - | chr8_1:8937610-8937629 | Msa1176750:CDS | 45.0% | |
| GCCTTCATAACTAGCATCTC+TGG | + | chr8_1:8937614-8937633 | None:intergenic | 45.0% | |
| AGATGCTAGTTATGAAGGCG+AGG | - | chr8_1:8937615-8937634 | Msa1176750:CDS | 45.0% | |
| TAGTTATGAAGGCGAGGCTA+TGG | - | chr8_1:8937621-8937640 | Msa1176750:CDS | 45.0% | |
| ATTGTGGTCTGCAGCGTGTA+AGG | - | chr8_1:8937483-8937502 | Msa1176750:CDS | 50.0% | |
| GCATTACAGGATACGAGTGC+TGG | - | chr8_1:8937549-8937568 | Msa1176750:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 8937442 | 8937666 | 8937442 | ID=Msa1176750;Name=Msa1176750 |
| chr8_1 | mRNA | 8937442 | 8937666 | 8937442 | ID=Msa1176750-mRNA-1;Parent=Msa1176750;Name=Msa1176750-mRNA-1;_AED=0.64;_eAED=1.00;_QI=0|0|0|0.5|1|1|2|0|67 |
| chr8_1 | exon | 8937533 | 8937666 | 8937533 | ID=Msa1176750-mRNA-1:exon:3037;Parent=Msa1176750-mRNA-1 |
| chr8_1 | exon | 8937442 | 8937508 | 8937442 | ID=Msa1176750-mRNA-1:exon:3036;Parent=Msa1176750-mRNA-1 |
| chr8_1 | CDS | 8937533 | 8937666 | 8937533 | ID=Msa1176750-mRNA-1:cds;Parent=Msa1176750-mRNA-1 |
| chr8_1 | CDS | 8937442 | 8937508 | 8937442 | ID=Msa1176750-mRNA-1:cds;Parent=Msa1176750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
