Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0107580 | Msa1176760 | 0.802844 | 4.626715e-49 | -8.615850e-47 |
| Msa0731550 | Msa1176760 | 0.800836 | 1.195484e-48 | -8.615850e-47 |
| Msa0766630 | Msa1176760 | 0.817870 | 2.634410e-52 | -8.615850e-47 |
| Msa0153910 | Msa1176760 | 0.843222 | 1.600472e-58 | -8.615850e-47 |
| Msa0214300 | Msa1176760 | 0.821821 | 3.293224e-53 | -8.615850e-47 |
| Msa1148050 | Msa1176760 | 0.805134 | 1.545959e-49 | -8.615850e-47 |
| Msa1176730 | Msa1176760 | 0.820202 | 7.769177e-53 | -8.615850e-47 |
| Msa1176750 | Msa1176760 | 0.904082 | 1.779080e-79 | -8.615850e-47 |
| Msa1176760 | Msa1176770 | 0.801161 | 1.025963e-48 | -8.615850e-47 |
| Msa1176760 | Msa1273230 | 0.801071 | 1.070393e-48 | -8.615850e-47 |
| Msa1176760 | Msa1277820 | 0.806573 | 7.702947e-50 | -8.615850e-47 |
| Msa1176760 | Msa1453720 | 0.808114 | 3.630204e-50 | -8.615850e-47 |
| Msa0885280 | Msa1176760 | 0.806802 | 6.890456e-50 | -8.615850e-47 |
| Msa0885290 | Msa1176760 | 0.809221 | 2.106424e-50 | -8.615850e-47 |
| Msa0907260 | Msa1176760 | 0.812673 | 3.765789e-51 | -8.615850e-47 |
| Msa0956270 | Msa1176760 | 0.805852 | 1.092839e-49 | -8.615850e-47 |
| Msa0959770 | Msa1176760 | 0.800825 | 1.201471e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCCAGAGGCTGTCATTGC+AGG | 0.324095 | 8_1:+8937772 | None:intergenic |
| TGTAACATTGTCGCACAAAC+TGG | 0.391092 | 8_1:+8937707 | None:intergenic |
| GATTTCGAGACACGACTTCA+CGG | 0.450107 | 8_1:+8937828 | None:intergenic |
| ATCCCTGCAATGACAGCCTC+TGG | 0.459212 | 8_1:-8937775 | Msa1176760:CDS |
| ACAATCTTTGTTCTCGTGAA+AGG | 0.464875 | 8_1:+8937732 | None:intergenic |
| CAATCTTTGTTCTCGTGAAA+GGG | 0.478114 | 8_1:+8937733 | None:intergenic |
| AGCCAGAGGCTGTCATTGCA+GGG | 0.564603 | 8_1:+8937773 | None:intergenic |
| CAAAGTGAAGAACAATATTG+TGG | 0.564949 | 8_1:-8937676 | Msa1176760:CDS |
| ACTATTAGTTCTTGAGCCAG+AGG | 0.608753 | 8_1:+8937759 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATTATATGGATAAATT+TGG | + | chr8_1:8937890-8937909 | None:intergenic | 10.0% |
| !! | ATAAATTATATGGATAAATT+TGG | + | chr8_1:8937890-8937909 | None:intergenic | 10.0% |
| !! | ATACTAAGTAGTTAAATATA+AGG | + | chr8_1:8938030-8938049 | None:intergenic | 15.0% |
| !! | ATACTAAGTAGTTAAATATA+AGG | + | chr8_1:8938030-8938049 | None:intergenic | 15.0% |
| !! | TATGCATCTCTAAATTAATT+TGG | + | chr8_1:8937682-8937701 | None:intergenic | 20.0% |
| !!! | AATTCAACTTTTTTCATTAG+TGG | + | chr8_1:8937712-8937731 | None:intergenic | 20.0% |
| !! | TACAGAAAAATTGAAATTGA+TGG | - | chr8_1:8937748-8937767 | Msa1176760:CDS | 20.0% |
| !! | ACTCAATTAGATATAATCAA+AGG | - | chr8_1:8937858-8937877 | Msa1176760:intron | 20.0% |
| !! | AATTATATGGATAAATTTGG+TGG | + | chr8_1:8937887-8937906 | None:intergenic | 20.0% |
| !! | TTGAAATATTCATAATCTCA+TGG | + | chr8_1:8937929-8937948 | None:intergenic | 20.0% |
| !!! | TTTGTGAAGTTCTTTTAAAT+AGG | + | chr8_1:8938164-8938183 | None:intergenic | 20.0% |
| !! | TATGCATCTCTAAATTAATT+TGG | + | chr8_1:8937682-8937701 | None:intergenic | 20.0% |
| !!! | AATTCAACTTTTTTCATTAG+TGG | + | chr8_1:8937712-8937731 | None:intergenic | 20.0% |
| !! | TACAGAAAAATTGAAATTGA+TGG | - | chr8_1:8937748-8937767 | Msa1176760:CDS | 20.0% |
| !! | ACTCAATTAGATATAATCAA+AGG | - | chr8_1:8937858-8937877 | Msa1176760:intron | 20.0% |
| !! | AATTATATGGATAAATTTGG+TGG | + | chr8_1:8937887-8937906 | None:intergenic | 20.0% |
| !! | TTGAAATATTCATAATCTCA+TGG | + | chr8_1:8937929-8937948 | None:intergenic | 20.0% |
| !!! | TTTGTGAAGTTCTTTTAAAT+AGG | + | chr8_1:8938164-8938183 | None:intergenic | 20.0% |
| ! | TCTGTTTGCATTTAAATTTG+AGG | + | chr8_1:8937793-8937812 | None:intergenic | 25.0% |
| !! | CAAACAGATTCTAGTTAATT+AGG | - | chr8_1:8937805-8937824 | Msa1176760:CDS | 25.0% |
| !! | TGAAAGCGCAATAAATTATA+TGG | + | chr8_1:8937900-8937919 | None:intergenic | 25.0% |
| ! | AAAATCATATGCCAATTTGA+TGG | - | chr8_1:8938181-8938200 | Msa1176760:intron | 25.0% |
| ! | ATATACTTCCTCAAAGAAAA+AGG | + | chr8_1:8938258-8938277 | None:intergenic | 25.0% |
| ! | TCTGTTTGCATTTAAATTTG+AGG | + | chr8_1:8937793-8937812 | None:intergenic | 25.0% |
| !! | CAAACAGATTCTAGTTAATT+AGG | - | chr8_1:8937805-8937824 | Msa1176760:CDS | 25.0% |
| !! | TGAAAGCGCAATAAATTATA+TGG | + | chr8_1:8937900-8937919 | None:intergenic | 25.0% |
| ! | AAAATCATATGCCAATTTGA+TGG | - | chr8_1:8938181-8938200 | Msa1176760:intron | 25.0% |
| ! | ATATACTTCCTCAAAGAAAA+AGG | + | chr8_1:8938258-8938277 | None:intergenic | 25.0% |
| TTTCAAATACGTCTCTTACT+CGG | - | chr8_1:8937943-8937962 | Msa1176760:intron | 30.0% | |
| ! | TTGCTTAACATCGTTTTACT+GGG | + | chr8_1:8938062-8938081 | None:intergenic | 30.0% |
| AAGCAACTAAGTTTGACAAA+AGG | - | chr8_1:8938076-8938095 | Msa1176760:intron | 30.0% | |
| TGACAAGTAAACCATCAAAT+TGG | + | chr8_1:8938195-8938214 | None:intergenic | 30.0% | |
| ! | CACTTTTAGTGCAGATTTTT+CGG | + | chr8_1:8938335-8938354 | None:intergenic | 30.0% |
| CAAAGTGAAGAACAATATTG+TGG | - | chr8_1:8938454-8938473 | Msa1176760:intron | 30.0% | |
| TTTCAAATACGTCTCTTACT+CGG | - | chr8_1:8937943-8937962 | Msa1176760:intron | 30.0% | |
| ! | TTGCTTAACATCGTTTTACT+GGG | + | chr8_1:8938062-8938081 | None:intergenic | 30.0% |
| AAGCAACTAAGTTTGACAAA+AGG | - | chr8_1:8938076-8938095 | Msa1176760:intron | 30.0% | |
| TGACAAGTAAACCATCAAAT+TGG | + | chr8_1:8938195-8938214 | None:intergenic | 30.0% | |
| ! | CACTTTTAGTGCAGATTTTT+CGG | + | chr8_1:8938335-8938354 | None:intergenic | 30.0% |
| CAAAGTGAAGAACAATATTG+TGG | - | chr8_1:8938454-8938473 | Msa1176760:intron | 30.0% | |
| ! | GTTGCTTAACATCGTTTTAC+TGG | + | chr8_1:8938063-8938082 | None:intergenic | 35.0% |
| ! | ATGATGCACCTTTTTCTTTG+AGG | - | chr8_1:8938247-8938266 | Msa1176760:intron | 35.0% |
| CAATCTTTGTTCTCGTGAAA+GGG | + | chr8_1:8938400-8938419 | None:intergenic | 35.0% | |
| ACAATCTTTGTTCTCGTGAA+AGG | + | chr8_1:8938401-8938420 | None:intergenic | 35.0% | |
| ! | GTTGCTTAACATCGTTTTAC+TGG | + | chr8_1:8938063-8938082 | None:intergenic | 35.0% |
| ! | ATGATGCACCTTTTTCTTTG+AGG | - | chr8_1:8938247-8938266 | Msa1176760:intron | 35.0% |
| CAATCTTTGTTCTCGTGAAA+GGG | + | chr8_1:8938400-8938419 | None:intergenic | 35.0% | |
| ACAATCTTTGTTCTCGTGAA+AGG | + | chr8_1:8938401-8938420 | None:intergenic | 35.0% | |
| TTTACTTGTCACTGCTGATC+TGG | - | chr8_1:8938204-8938223 | Msa1176760:intron | 40.0% | |
| ! | ACTATTAGTTCTTGAGCCAG+AGG | + | chr8_1:8938374-8938393 | None:intergenic | 40.0% |
| TGTAACATTGTCGCACAAAC+TGG | + | chr8_1:8938426-8938445 | None:intergenic | 40.0% | |
| TTTACTTGTCACTGCTGATC+TGG | - | chr8_1:8938204-8938223 | Msa1176760:intron | 40.0% | |
| ! | ACTATTAGTTCTTGAGCCAG+AGG | + | chr8_1:8938374-8938393 | None:intergenic | 40.0% |
| TGTAACATTGTCGCACAAAC+TGG | + | chr8_1:8938426-8938445 | None:intergenic | 40.0% | |
| GATTTCGAGACACGACTTCA+CGG | + | chr8_1:8938305-8938324 | None:intergenic | 45.0% | |
| GATTTCGAGACACGACTTCA+CGG | + | chr8_1:8938305-8938324 | None:intergenic | 45.0% | |
| ATCCCTGCAATGACAGCCTC+TGG | - | chr8_1:8938355-8938374 | Msa1176760:intron | 55.0% | |
| AGCCAGAGGCTGTCATTGCA+GGG | + | chr8_1:8938360-8938379 | None:intergenic | 55.0% | |
| ATCCCTGCAATGACAGCCTC+TGG | - | chr8_1:8938355-8938374 | Msa1176760:intron | 55.0% | |
| AGCCAGAGGCTGTCATTGCA+GGG | + | chr8_1:8938360-8938379 | None:intergenic | 55.0% | |
| GAGCCAGAGGCTGTCATTGC+AGG | + | chr8_1:8938361-8938380 | None:intergenic | 60.0% | |
| GAGCCAGAGGCTGTCATTGC+AGG | + | chr8_1:8938361-8938380 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 8937669 | 8938483 | 8937669 | ID=Msa1176760;Name=Msa1176760 |
| chr8_1 | mRNA | 8937669 | 8938483 | 8937669 | ID=Msa1176760-mRNA-1;Parent=Msa1176760;Name=Msa1176760-mRNA-1;_AED=0.81;_eAED=1.00;_QI=0|0|0|0.5|0|0|2|0|64 |
| chr8_1 | exon | 8938480 | 8938483 | 8938480 | ID=Msa1176760-mRNA-1:exon:3039;Parent=Msa1176760-mRNA-1 |
| chr8_1 | exon | 8937669 | 8937859 | 8937669 | ID=Msa1176760-mRNA-1:exon:3038;Parent=Msa1176760-mRNA-1 |
| chr8_1 | CDS | 8938480 | 8938483 | 8938480 | ID=Msa1176760-mRNA-1:cds;Parent=Msa1176760-mRNA-1 |
| chr8_1 | CDS | 8937669 | 8937859 | 8937669 | ID=Msa1176760-mRNA-1:cds;Parent=Msa1176760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
