AlfalfaGEDB | Msa1178450

M. sativa cultivar zhongmu-4 / Msa1178450

KEGG: K02907
GO: -
PFAM: PF00327.23

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1178450	I3S856	100.000	106	0	0	1	106	1	106	1.01e-72	220

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1178450	Msa1352540	0.803973	2.699376e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1178450	MtrunA17_Chr8g0342041	100.000	106	0	0	1	106	1	106	1.95e-76	220
Msa1178450	MtrunA17_Chr8g0387721	98.113	106	2	0	1	106	1	106	3.18e-75	217

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1178450	AT5G55140.1	64.762	105	36	1	3	106	1	105	6.71e-46	144

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Find 60 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAACTTATAATCTAGTT+GGG	0.418471	8_1:+11163197	Msa1178450:three_prime_UTR
AACTCTAGTGAGGGGTATTC+CGG	0.253922	8_1:+11162621	Msa1178450:CDS
AAGCAGAAGAAGCAGGTTGA+TGG	0.435151	8_1:-11162936	None:intergenic
AAGTTTCTCACTGTTTGTGG+CGG	0.625247	8_1:-11160499	None:intergenic
AATATTCTATTTAAAGACAG+AGG	0.604664	8_1:-11163235	None:intergenic
ACGGAGGACGAAGAGCTCGG+TGG	0.687356	8_1:-11162903	None:intergenic
ACTAGAGTTATATAAAGATT+GGG	0.420712	8_1:-11162607	None:intergenic
ACTCTAGTGAGGGGTATTCC+GGG	0.407993	8_1:+11162622	Msa1178450:CDS
ACTTTGGAAGCACTACGTCT+CGG	0.676793	8_1:+11162667	Msa1178450:CDS
AGAAAGTTTCTCACTGTTTG+TGG	0.430411	8_1:-11160502	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATCTCTCGAAATACAGTT+GGG	-	chr8_1:11161191-11161210	None:intergenic	30.0%
CTATATGCAAATGGAAAAAG+TGG	-	chr8_1:11161423-11161442	None:intergenic	30.0%
GTGTAGAAATCTCAATATTG+AGG	+	chr8_1:11161546-11161565	Msa1178450:intron	30.0%
GTAGAAATCTCAATATTGAG+GGG	+	chr8_1:11161548-11161567	Msa1178450:intron	30.0%
AAAGCATAGTAGCAACAAAA+TGG	+	chr8_1:11162339-11162358	Msa1178450:intron	30.0%
CTTTATATAACTCTAGTGAG+GGG	+	chr8_1:11162613-11162632	Msa1178450:CDS	30.0%
CAATGCAAATAAACCATATG+AGG	-	chr8_1:11163036-11163055	None:intergenic	30.0%
AGAAAGTTTCTCACTGTTTG+TGG	-	chr8_1:11160505-11160524	None:intergenic	35.0%
TTATGGTAGCAAATCGCATT+GGG	-	chr8_1:11160581-11160600	None:intergenic	35.0%
ATTATGGTAGCAAATCGCAT+TGG	-	chr8_1:11160582-11160601	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	11160383	11163327	11160383	ID=Msa1178450;Name=Msa1178450
chr8_1	mRNA	11160383	11163327	11160383	ID=Msa1178450-mRNA-1;Parent=Msa1178450;Name=Msa1178450-mRNA-1;_AED=0.05;_eAED=0.05;_QI=136\|1\|1\|1\|0.5\|0.66\|3\|367\|106
chr8_1	exon	11160383	11160510	11160383	ID=Msa1178450-mRNA-1:exon:3906;Parent=Msa1178450-mRNA-1
chr8_1	exon	11162545	11162750	11162545	ID=Msa1178450-mRNA-1:exon:3907;Parent=Msa1178450-mRNA-1
chr8_1	exon	11162838	11163327	11162838	ID=Msa1178450-mRNA-1:exon:3908;Parent=Msa1178450-mRNA-1
chr8_1	five_prime_UTR	11160383	11160510	11160383	ID=Msa1178450-mRNA-1:five_prime_utr;Parent=Msa1178450-mRNA-1
chr8_1	five_prime_UTR	11162545	11162552	11162545	ID=Msa1178450-mRNA-1:five_prime_utr;Parent=Msa1178450-mRNA-1
chr8_1	CDS	11162553	11162750	11162553	ID=Msa1178450-mRNA-1:cds;Parent=Msa1178450-mRNA-1
chr8_1	CDS	11162838	11162960	11162838	ID=Msa1178450-mRNA-1:cds;Parent=Msa1178450-mRNA-1
chr8_1	three_prime_UTR	11162961	11163327	11162961	ID=Msa1178450-mRNA-1:three_prime_utr;Parent=Msa1178450-mRNA-1

Gene Sequence	>Msa1178450 ATGAGCATGAATGCGTTCAAGGCATTTAAGTCCTGTGTCCCAATTGCTTATAGCCCCAATCTTTATATAACTCTAGTGAGGGGTATTCCGGGGACCAGGAAGCAACATAGGCGCACTTTGGAAGCACTACGTCTCGGCAAATGCAACCGAACTGTCATGCGATGGAACACACCTACTGTTAGGGGAATGATCCAGCAGGTTAAGAGGCTAGTTGTTGTTGAGACAGAGGAGATGTACAAGGCCCGCAAAGAAAAGGAGGAAGCCCACCGAGCTCTTCGTCCTCCGTTGGTCATAAACCATCAACCTGCTTCTTCTGCTTAG
Protein sequence	>Msa1178450 MSMNAFKAFKSCVPIAYSPNLYITLVRGIPGTRKQHRRTLEALRLGKCNRTVMRWNTPTVRGMIQQVKRLVVVETEEMYKARKEKEEAHRALRPPLVINHQPASSA*