AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1180160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAACACCTTTGCTCTTAA+TGG 0.113419 8_1:-13943954 None:intergenic
ATTGATTTCATTAACTTTAA+AGG 0.177612 8_1:-13945592 None:intergenic
CCATGTTTATAAATATAATT+TGG 0.195747 8_1:+13945620 Msa1180160:three_prime_UTR
TCGAGTGTTACCTGCATCTT+TGG 0.257314 8_1:-13945479 None:intergenic
AGGGATGAGTTGTCTACTTT+TGG 0.272750 8_1:+13942601 Msa1180160:CDS
CTTGGCACTCCATAGTAAAA+TGG 0.313706 8_1:-13945407 None:intergenic
TCTCACCTCACAATCAATTC+TGG 0.358267 8_1:-13942663 None:intergenic
GCAAAGGTGTTTCATCTAAC+GGG 0.388326 8_1:+13943963 Msa1180160:CDS
TTCCTCAGAACTTGCACTAC+AGG 0.406661 8_1:-13941429 None:intergenic
TAGAGTCATGTGAGTAATCT+TGG 0.418763 8_1:-13945425 None:intergenic
AATTTACCAGCAGCCATTGC+AGG 0.432252 8_1:-13941471 None:intergenic
CACAGGGCATGTTTGAACTT+AGG 0.461649 8_1:-13941404 None:intergenic
AACTTGCACTACAGGGTCAC+AGG 0.463707 8_1:-13941421 None:intergenic
GTTTCTCCTGCAATGGCTGC+TGG 0.473825 8_1:+13941465 Msa1180160:CDS
CCAAATTATATTTATAAACA+TGG 0.485194 8_1:-13945620 None:intergenic
TGTTCTCGTTTCTCCTGCAA+TGG 0.507124 8_1:+13941458 Msa1180160:CDS
AGCAAAGGTGTTTCATCTAA+CGG 0.514730 8_1:+13943962 Msa1180160:CDS
GATTAACTATTTCAGGTGTA+GGG 0.516528 8_1:+13942582 Msa1180160:intron
ATGATTTGATTACAGTGTGA+AGG 0.537265 8_1:+13945359 Msa1180160:intron
TGCAATCAGTCCAAAGATGC+AGG 0.540241 8_1:+13945469 Msa1180160:CDS
TGGTGGTGATAACACTGGTT+GGG 0.555703 8_1:-13942643 None:intergenic
GGTAATAATCCCTAAAACAA+GGG 0.558384 8_1:-13945386 None:intergenic
TGGTAATAATCCCTAAAACA+AGG 0.569912 8_1:-13945387 None:intergenic
TCCTCAGAACTTGCACTACA+GGG 0.584365 8_1:-13941428 None:intergenic
ATTGAAGCCATTAAGAGCAA+AGG 0.604940 8_1:+13943947 Msa1180160:CDS
CTCACATGACTCTAGTGTGA+AGG 0.613928 8_1:+13945434 Msa1180160:CDS
CTGGTGGTGATAACACTGGT+TGG 0.618340 8_1:-13942644 None:intergenic
CACCTCACAATCAATTCTGG+TGG 0.620720 8_1:-13942660 None:intergenic
GGTGATAACACTGGTTGGGA+AGG 0.621015 8_1:-13942639 None:intergenic
CATGACTCTAGTGTGAAGGC+TGG 0.622131 8_1:+13945438 Msa1180160:CDS
CACCACCAGAATTGATTGTG+AGG 0.625640 8_1:+13942658 Msa1180160:CDS
CAAAGGTGTTTCATCTAACG+GGG 0.644795 8_1:+13943964 Msa1180160:CDS
ACTTGCACTACAGGGTCACA+GGG 0.655163 8_1:-13941420 None:intergenic
ACCCTGTAGTGCAAGTTCTG+AGG 0.686102 8_1:+13941427 Msa1180160:CDS
AATTCTGGTGGTGATAACAC+TGG 0.692964 8_1:-13942648 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGAGAAAAATAAAAAA+CGG + chr8_1:13941783-13941802 Msa1180160:intron 10.0%
!! AATAATTAAGTAATTCAAAT+AGG - chr8_1:13942138-13942157 None:intergenic 10.0%
!!! AGTTTAAATTTTATTTTGAA+AGG - chr8_1:13942887-13942906 None:intergenic 10.0%
!!! TTTAAATGAAACAAATTTTT+TGG + chr8_1:13943677-13943696 Msa1180160:intron 10.0%
!! TATAATAATATAAAATTGCT+TGG + chr8_1:13944345-13944364 Msa1180160:intron 10.0%
!! TTATTATTATATCATAGTTA+TGG + chr8_1:13944436-13944455 Msa1180160:intron 10.0%
!!! TGTAAAAATAATTGAATTTT+AGG - chr8_1:13944770-13944789 None:intergenic 10.0%
!!! TTAAAATACTGCATTATTTT+AGG - chr8_1:13941505-13941524 None:intergenic 15.0%
!!! AAAAGAAACAATTTAAGATA+GGG - chr8_1:13941965-13941984 None:intergenic 15.0%
!! AATGATACAAAATATGATAA+GGG - chr8_1:13942098-13942117 None:intergenic 15.0%
!!! CAAATATGAAATTTCATTTT+TGG + chr8_1:13942394-13942413 Msa1180160:intron 15.0%
!! GTAAATATAGTTTAACAAAA+AGG - chr8_1:13942799-13942818 None:intergenic 15.0%
!!! ATTATTTTGATTTGTACATA+TGG - chr8_1:13943481-13943500 None:intergenic 15.0%
!! TACCAAAAAAAAAATATCTT+GGG - chr8_1:13944572-13944591 None:intergenic 15.0%
!! ATACCAAAAAAAAAATATCT+TGG - chr8_1:13944573-13944592 None:intergenic 15.0%
!!! TAGTGCTTTATTTATTTTTT+TGG + chr8_1:13944742-13944761 Msa1180160:intron 15.0%
!!! AGTGCTTTATTTATTTTTTT+GGG + chr8_1:13944743-13944762 Msa1180160:intron 15.0%
!!! ATTGATTTCATTAACTTTAA+AGG - chr8_1:13945595-13945614 None:intergenic 15.0%
!! CCAAATTATATTTATAAACA+TGG - chr8_1:13945623-13945642 None:intergenic 15.0%
!! CCATGTTTATAAATATAATT+TGG + chr8_1:13945620-13945639 Msa1180160:three_prime_UTR 15.0%
!! CTACTAACTACAATTAATAA+GGG - chr8_1:13941674-13941693 None:intergenic 20.0%
!! TCTACTAACTACAATTAATA+AGG - chr8_1:13941675-13941694 None:intergenic 20.0%
!! TGGATGAAATTCAATAAATA+TGG + chr8_1:13941705-13941724 Msa1180160:intron 20.0%
!! GGATGAAATTCAATAAATAT+GGG + chr8_1:13941706-13941725 Msa1180160:intron 20.0%
!!! GAAAAGAAACAATTTAAGAT+AGG - chr8_1:13941966-13941985 None:intergenic 20.0%
!! TCATATTCTTTGTAAAAACT+AGG - chr8_1:13942047-13942066 None:intergenic 20.0%
!! GAATGATACAAAATATGATA+AGG - chr8_1:13942099-13942118 None:intergenic 20.0%
!! GAATTACTTAATTATTGACA+TGG + chr8_1:13942142-13942161 Msa1180160:intron 20.0%
!!! TACAAAAACATTTTATAGCA+AGG + chr8_1:13942227-13942246 Msa1180160:intron 20.0%
!! ATATTATGGCTTAAAATCAT+AGG + chr8_1:13942487-13942506 Msa1180160:intron 20.0%
!!! CTACTTTTGGAATATTTTTA+TGG + chr8_1:13942614-13942633 Msa1180160:CDS 20.0%
!! AGATAATTCAAGAAAAATGT+TGG + chr8_1:13942715-13942734 Msa1180160:intron 20.0%
!!! TAAACTATATTTACACCTTT+TGG + chr8_1:13942805-13942824 Msa1180160:intron 20.0%
!!! AAATCAACAAGTTTTGTTAA+TGG - chr8_1:13943061-13943080 None:intergenic 20.0%
!! AAGAAAACAATTTGTAAAAG+CGG - chr8_1:13943822-13943841 None:intergenic 20.0%
!! AATATACAAGTAAACCTATA+TGG - chr8_1:13944077-13944096 None:intergenic 20.0%
!! TATAGGTTTACTTGTATATT+AGG + chr8_1:13944077-13944096 Msa1180160:intron 20.0%
!!! TATATTAGGTTTGTCTTATT+TGG - chr8_1:13944204-13944223 None:intergenic 20.0%
!! ATATTTCAACTATACAGTAA+AGG - chr8_1:13944254-13944273 None:intergenic 20.0%
!!! GTGCTTTATTTATTTTTTTG+GGG + chr8_1:13944744-13944763 Msa1180160:intron 20.0%
!! AAATTCAACATTAAGATTCA+GGG - chr8_1:13944837-13944856 None:intergenic 20.0%
!! TTCAAAATCTATTTACCTTT+CGG + chr8_1:13944921-13944940 Msa1180160:intron 20.0%
!! TTTATTACCAAAATGATCAA+TGG - chr8_1:13945130-13945149 None:intergenic 20.0%
!! TTGAATATAACCAATAAGAA+CGG + chr8_1:13945268-13945287 Msa1180160:intron 20.0%
!!! TTTTTCAAACAAAGATTTGA+AGG - chr8_1:13945548-13945567 None:intergenic 20.0%
! AAACGACTAATATTAAGAGA+CGG - chr8_1:13941573-13941592 None:intergenic 25.0%
! CTCTTAATATTAGTCGTTTA+AGG + chr8_1:13941574-13941593 Msa1180160:intron 25.0%
!!! ATTAGTTGTGTTGATTTTGA+TGG + chr8_1:13941622-13941641 Msa1180160:intron 25.0%
!! AGTTAGTAGAGTATTTAAGT+TGG + chr8_1:13941685-13941704 Msa1180160:intron 25.0%
! AAACATAATAAATGCTGCAT+TGG + chr8_1:13941742-13941761 Msa1180160:intron 25.0%
! AAAAGACAATTATTGTGAGA+CGG + chr8_1:13941808-13941827 Msa1180160:intron 25.0%
! ATACAAAATATGATAAGGGA+TGG - chr8_1:13942094-13942113 None:intergenic 25.0%
!!! ACTTTTATAGCATTGATTGA+TGG + chr8_1:13942322-13942341 Msa1180160:intron 25.0%
!!! TTTCATTTTTGGTGATTGAT+TGG + chr8_1:13942405-13942424 Msa1180160:intron 25.0%
! TATATTGCCAACATGATAAT+TGG - chr8_1:13942690-13942709 None:intergenic 25.0%
! CATGTTTATTCAAGAATTCA+GGG - chr8_1:13942772-13942791 None:intergenic 25.0%
! TCATGTTTATTCAAGAATTC+AGG - chr8_1:13942773-13942792 None:intergenic 25.0%
! TGAAAGGAAAGATGATTAAA+GGG - chr8_1:13942871-13942890 None:intergenic 25.0%
! TTGAAAGGAAAGATGATTAA+AGG - chr8_1:13942872-13942891 None:intergenic 25.0%
!! GACTAATTGTTGTATTCTTT+TGG + chr8_1:13942912-13942931 Msa1180160:intron 25.0%
! ATAAGACAAACTTGTATAGT+AGG + chr8_1:13943279-13943298 Msa1180160:intron 25.0%
!!! TAGCTTTGTAGGTATTATAT+GGG + chr8_1:13943318-13943337 Msa1180160:intron 25.0%
!! GTTGAACATTTTCAAATGTA+TGG - chr8_1:13943426-13943445 None:intergenic 25.0%
!! TGGTTAAGATTATTGATTGT+TGG + chr8_1:13943562-13943581 Msa1180160:intron 25.0%
!! ATTATTGATTGTTGGAAGAT+GGG + chr8_1:13943570-13943589 Msa1180160:intron 25.0%
! AAACAATTGAGACATTAAAG+AGG - chr8_1:13943637-13943656 None:intergenic 25.0%
! AAATCAACACATTCCTATTT+CGG - chr8_1:13943731-13943750 None:intergenic 25.0%
!!! AAATAGGAATGTGTTGATTT+TGG + chr8_1:13943731-13943750 Msa1180160:intron 25.0%
! AGAAAACAATTTGTAAAAGC+GGG - chr8_1:13943821-13943840 None:intergenic 25.0%
! TTTCACTAAGCATACTTATT+TGG + chr8_1:13944011-13944030 Msa1180160:intron 25.0%
!!! GTCTTATTTGGTTTCATTTT+GGG - chr8_1:13944192-13944211 None:intergenic 25.0%
!!! TGTCTTATTTGGTTTCATTT+TGG - chr8_1:13944193-13944212 None:intergenic 25.0%
! CATGCATATTGCAATATATT+AGG - chr8_1:13944218-13944237 None:intergenic 25.0%
!! CAGATTTTATGACTTTAGTT+AGG - chr8_1:13944288-13944307 None:intergenic 25.0%
! TTATGGTTAGTGAATTTGAA+TGG + chr8_1:13944453-13944472 Msa1180160:intron 25.0%
! TTTCCATATTCAATCTTAAC+AGG + chr8_1:13944489-13944508 Msa1180160:intron 25.0%
! TTCCATATTCAATCTTAACA+GGG + chr8_1:13944490-13944509 Msa1180160:intron 25.0%
!! AAAGCGAAAATTTTTCATGT+TGG + chr8_1:13944625-13944644 Msa1180160:intron 25.0%
! TTAATAAAAAGGACTCAAGA+GGG - chr8_1:13944709-13944728 None:intergenic 25.0%
! ATTAATAAAAAGGACTCAAG+AGG - chr8_1:13944710-13944729 None:intergenic 25.0%
! CAAATTCAACATTAAGATTC+AGG - chr8_1:13944838-13944857 None:intergenic 25.0%
!! ATAGATTTTGAATAGACGTT+GGG - chr8_1:13944913-13944932 None:intergenic 25.0%
!! AATAGATTTTGAATAGACGT+TGG - chr8_1:13944914-13944933 None:intergenic 25.0%
!! GGATTATTACCATTTTACTA+TGG + chr8_1:13945398-13945417 Msa1180160:CDS 25.0%
AGGTTTATGCACGATTATTA+AGG + chr8_1:13941594-13941613 Msa1180160:intron 30.0%
! GGATGATTCATTATTTTCGA+TGG - chr8_1:13941944-13941963 None:intergenic 30.0%
GGATGGCATTAAAATCATTA+GGG - chr8_1:13942077-13942096 None:intergenic 30.0%
! CATTTTATAGCAAGGATAGA+TGG + chr8_1:13942235-13942254 Msa1180160:intron 30.0%
GTAAGCGTGCATATATATTA+TGG + chr8_1:13942473-13942492 Msa1180160:intron 30.0%
GTCTTCTGATTAACTATTTC+AGG + chr8_1:13942575-13942594 Msa1180160:intron 30.0%
! TGATTAACTATTTCAGGTGT+AGG + chr8_1:13942581-13942600 Msa1180160:intron 30.0%
! GATTAACTATTTCAGGTGTA+GGG + chr8_1:13942582-13942601 Msa1180160:intron 30.0%
! TTTTCTACAATCTGTCCAAA+AGG - chr8_1:13942823-13942842 None:intergenic 30.0%
AAAGATGATTAAAGGGCAAA+AGG - chr8_1:13942864-13942883 None:intergenic 30.0%
AGTTGTGTAAACCTATGAAT+TGG + chr8_1:13942982-13943001 Msa1180160:intron 30.0%
! CTTCTTTGTTTCCAATTCAT+AGG - chr8_1:13942996-13943015 None:intergenic 30.0%
! CATAAACAAGAGAGTATGAA+GGG + chr8_1:13943198-13943217 Msa1180160:intron 30.0%
!! AACCTATATTGTAGCTTTGT+AGG + chr8_1:13943307-13943326 Msa1180160:intron 30.0%
TACCTACAAAGCTACAATAT+AGG - chr8_1:13943312-13943331 None:intergenic 30.0%
!! GTAGCTTTGTAGGTATTATA+TGG + chr8_1:13943317-13943336 Msa1180160:intron 30.0%
! AGCTTTGTAGGTATTATATG+GGG + chr8_1:13943319-13943338 Msa1180160:intron 30.0%
AAATTAACATCCACTTGCAA+TGG - chr8_1:13943383-13943402 None:intergenic 30.0%
ATTTGAAAATGTTCAACGCA+GGG + chr8_1:13943429-13943448 Msa1180160:intron 30.0%
! TGATTTGTACATATGGACTA+TGG - chr8_1:13943474-13943493 None:intergenic 30.0%
AAAAAATCCCACTGTTCAAA+CGG - chr8_1:13943534-13943553 None:intergenic 30.0%
! GATTATTGATTGTTGGAAGA+TGG + chr8_1:13943569-13943588 Msa1180160:intron 30.0%
! TTATTGATTGTTGGAAGATG+GGG + chr8_1:13943571-13943590 Msa1180160:intron 30.0%
ATTAAAGAGGACACAATAGT+TGG - chr8_1:13943624-13943643 None:intergenic 30.0%
TTCATTTAAACGTATCACAG+AGG - chr8_1:13943667-13943686 None:intergenic 30.0%
!! CAAAGTTTTATCACCGAAAT+AGG + chr8_1:13943715-13943734 Msa1180160:intron 30.0%
! TTGTTTTCTTTACTGTCATC+TGG + chr8_1:13943832-13943851 Msa1180160:intron 30.0%
!!! GGCCCAAGATATTTTTTTTT+TGG + chr8_1:13944567-13944586 Msa1180160:intron 30.0%
! GAAATCTGGAATTTCATTTC+TGG + chr8_1:13944948-13944967 Msa1180160:intron 30.0%
TTGTTGCGAAATTTCTTCTA+AGG + chr8_1:13944991-13945010 Msa1180160:intron 30.0%
CAATATGTGGCTTAAAATCA+TGG + chr8_1:13945033-13945052 Msa1180160:intron 30.0%
! TCTCACCTTACAATCAATTT+TGG - chr8_1:13945106-13945125 None:intergenic 30.0%
ATATTAAAATCTTGCTGACG+TGG + chr8_1:13945211-13945230 Msa1180160:intron 30.0%
TTTATTTCAGCCGTTCTTAT+TGG - chr8_1:13945281-13945300 None:intergenic 30.0%
CACTGTAATCAAATCATTGA+AGG - chr8_1:13945356-13945375 None:intergenic 30.0%
ATGATTTGATTACAGTGTGA+AGG + chr8_1:13945359-13945378 Msa1180160:intron 30.0%
GGTAATAATCCCTAAAACAA+GGG - chr8_1:13945389-13945408 None:intergenic 30.0%
TGGTAATAATCCCTAAAACA+AGG - chr8_1:13945390-13945409 None:intergenic 30.0%
GACTAATATTAAGAGACGGA+GGG - chr8_1:13941569-13941588 None:intergenic 35.0%
! AACCAGAGACTTTGAAAGAA+AGG - chr8_1:13941861-13941880 None:intergenic 35.0%
GGGATGGCATTAAAATCATT+AGG - chr8_1:13942078-13942097 None:intergenic 35.0%
GTATCATTCCTTCCAAAAAG+TGG + chr8_1:13942110-13942129 Msa1180160:intron 35.0%
!!! TTCAAATAGGTGCCACTTTT+TGG - chr8_1:13942125-13942144 None:intergenic 35.0%
CGTAGTACAAAGAGCTAAAT+TGG - chr8_1:13942268-13942287 None:intergenic 35.0%
GTGAGAACCAATTATCATGT+TGG + chr8_1:13942680-13942699 Msa1180160:intron 35.0%
!! AATGTTGGTTTGTTTGCTTC+AGG + chr8_1:13942730-13942749 Msa1180160:intron 35.0%
!! TCTAACTTTGATGATGCATG+TGG - chr8_1:13943099-13943118 None:intergenic 35.0%
GAAGACAAATCTCAACATGA+AGG + chr8_1:13943136-13943155 Msa1180160:intron 35.0%
! GCATAAACAAGAGAGTATGA+AGG + chr8_1:13943197-13943216 Msa1180160:intron 35.0%
TTGTAGGTATTATATGGGGA+AGG + chr8_1:13943323-13943342 Msa1180160:intron 35.0%
AATGACAAAACCATTGCAAG+TGG + chr8_1:13943370-13943389 Msa1180160:intron 35.0%
CATTTGAAAATGTTCAACGC+AGG + chr8_1:13943428-13943447 Msa1180160:intron 35.0%
TTTGAAAATGTTCAACGCAG+GGG + chr8_1:13943430-13943449 Msa1180160:intron 35.0%
TATGGAGTACATGTTATGGT+GGG - chr8_1:13943456-13943475 None:intergenic 35.0%
AAAAATCCCACTGTTCAAAC+GGG - chr8_1:13943533-13943552 None:intergenic 35.0%
TGGGGAATAATTCACTTGAT+TGG + chr8_1:13943589-13943608 Msa1180160:intron 35.0%
AAACAATTTGTAAAAGCGGG+TGG - chr8_1:13943818-13943837 None:intergenic 35.0%
ATTGAAGCCATTAAGAGCAA+AGG + chr8_1:13943947-13943966 Msa1180160:CDS 35.0%
TGAAACACCTTTGCTCTTAA+TGG - chr8_1:13943957-13943976 None:intergenic 35.0%
!! AGCAAAGGTGTTTCATCTAA+CGG + chr8_1:13943962-13943981 Msa1180160:CDS 35.0%
AAGTGTCACACTATCACATA+AGG - chr8_1:13944318-13944337 None:intergenic 35.0%
!!! ATGTTTTAATGCGAGGTCAT+GGG + chr8_1:13944676-13944695 Msa1180160:intron 35.0%
ATCCCTTAAGAGACTAAAAC+TGG + chr8_1:13944789-13944808 Msa1180160:intron 35.0%
TCCCTTAAGAGACTAAAACT+GGG + chr8_1:13944790-13944809 Msa1180160:intron 35.0%
TATGTGATGCATGCAATATG+TGG + chr8_1:13945020-13945039 Msa1180160:intron 35.0%
!!! CAATTTTGGTGGTGATAAAC+TGG - chr8_1:13945092-13945111 None:intergenic 35.0%
CACCACCAAAATTGATTGTA+AGG + chr8_1:13945098-13945117 Msa1180160:intron 35.0%
! CACCTTACAATCAATTTTGG+TGG - chr8_1:13945103-13945122 None:intergenic 35.0%
! GTGAGAACCATTGATCATTT+TGG + chr8_1:13945120-13945139 Msa1180160:intron 35.0%
!!! TGAAGGTAAACCCTTGTTTT+AGG + chr8_1:13945376-13945395 Msa1180160:CDS 35.0%
!!! GAAGGTAAACCCTTGTTTTA+GGG + chr8_1:13945377-13945396 Msa1180160:CDS 35.0%
TAGAGTCATGTGAGTAATCT+TGG - chr8_1:13945428-13945447 None:intergenic 35.0%
CGACTAATATTAAGAGACGG+AGG - chr8_1:13941570-13941589 None:intergenic 40.0%
AGACAATTATTGTGAGACGG+AGG + chr8_1:13941811-13941830 Msa1180160:intron 40.0%
GACAATTATTGTGAGACGGA+GGG + chr8_1:13941812-13941831 Msa1180160:intron 40.0%
CTCCTTTCTTTCAAAGTCTC+TGG + chr8_1:13941856-13941875 Msa1180160:intron 40.0%
!! TAAGAGATGCTTCTAGAGCT+TGG - chr8_1:13941998-13942017 None:intergenic 40.0%
!!! AATAGGTGCCACTTTTTGGA+AGG - chr8_1:13942121-13942140 None:intergenic 40.0%
! AGGGATGAGTTGTCTACTTT+TGG + chr8_1:13942601-13942620 Msa1180160:CDS 40.0%
! AATTCTGGTGGTGATAACAC+TGG - chr8_1:13942651-13942670 None:intergenic 40.0%
TCTCACCTCACAATCAATTC+TGG - chr8_1:13942666-13942685 None:intergenic 40.0%
ACTAACTCATGAGACTCTTG+AGG - chr8_1:13942965-13942984 None:intergenic 40.0%
! TGATGATGCATGTGGTCTAT+TGG - chr8_1:13943091-13943110 None:intergenic 40.0%
GCATCATCAAAGTTAGAGCA+TGG + chr8_1:13943102-13943121 Msa1180160:intron 40.0%
CTATGGAGTACATGTTATGG+TGG - chr8_1:13943457-13943476 None:intergenic 40.0%
GGACTATGGAGTACATGTTA+TGG - chr8_1:13943460-13943479 None:intergenic 40.0%
AACTATTCCCGTTTGAACAG+TGG + chr8_1:13943523-13943542 Msa1180160:intron 40.0%
ACTATTCCCGTTTGAACAGT+GGG + chr8_1:13943524-13943543 Msa1180160:intron 40.0%
!!! GTGGGATTTTTTTAGCACTG+TGG + chr8_1:13943542-13943561 Msa1180160:intron 40.0%
! ATTGTGTCATTCTCTGATGC+AGG + chr8_1:13943895-13943914 Msa1180160:intron 40.0%
!! GCAAAGGTGTTTCATCTAAC+GGG + chr8_1:13943963-13943982 Msa1180160:CDS 40.0%
!! CAAAGGTGTTTCATCTAACG+GGG + chr8_1:13943964-13943983 Msa1180160:CDS 40.0%
TCAGCATACAGAATAGGAGA+GGG - chr8_1:13944044-13944063 None:intergenic 40.0%
GGCCCTGTTAAGATTGAATA+TGG - chr8_1:13944495-13944514 None:intergenic 40.0%
ACCTCGCATTAAAACATGAG+AGG - chr8_1:13944673-13944692 None:intergenic 40.0%
!!! CATGTTTTAATGCGAGGTCA+TGG + chr8_1:13944675-13944694 Msa1180160:intron 40.0%
!! GCCCAGTTTTAGTCTCTTAA+GGG - chr8_1:13944794-13944813 None:intergenic 40.0%
!! GGCCTACGAATTTGTTAAGA+CGG + chr8_1:13944811-13944830 Msa1180160:intron 40.0%
TACCTTTCGGAGTTGAAATC+TGG + chr8_1:13944934-13944953 Msa1180160:intron 40.0%
TTCCAGATTTCAACTCCGAA+AGG - chr8_1:13944939-13944958 None:intergenic 40.0%
CTTGGCACTCCATAGTAAAA+TGG - chr8_1:13945410-13945429 None:intergenic 40.0%
CACAGGGCATGTTTGAACTT+AGG - chr8_1:13941407-13941426 None:intergenic 45.0%
TCCTCAGAACTTGCACTACA+GGG - chr8_1:13941431-13941450 None:intergenic 45.0%
TTCCTCAGAACTTGCACTAC+AGG - chr8_1:13941432-13941451 None:intergenic 45.0%
TGTTCTCGTTTCTCCTGCAA+TGG + chr8_1:13941458-13941477 Msa1180160:CDS 45.0%
AATTTACCAGCAGCCATTGC+AGG - chr8_1:13941474-13941493 None:intergenic 45.0%
! TGGTGGTGATAACACTGGTT+GGG - chr8_1:13942646-13942665 None:intergenic 45.0%
CACCACCAGAATTGATTGTG+AGG + chr8_1:13942658-13942677 Msa1180160:CDS 45.0%
CACCTCACAATCAATTCTGG+TGG - chr8_1:13942663-13942682 None:intergenic 45.0%
! ATGCCTTCATGTTGCTGTTG+TGG + chr8_1:13942936-13942955 Msa1180160:intron 45.0%
CAACCACAACAGCAACATGA+AGG - chr8_1:13942942-13942961 None:intergenic 45.0%
TGATGCATGTGGTCTATTGG+AGG - chr8_1:13943088-13943107 None:intergenic 45.0%
! AAGAGAGTATGAAGGGTCAG+AGG + chr8_1:13943205-13943224 Msa1180160:intron 45.0%
! AGAGAGTATGAAGGGTCAGA+GGG + chr8_1:13943206-13943225 Msa1180160:intron 45.0%
! GGGCTGCTGAATTTTCTTTG+TGG + chr8_1:13943984-13944003 Msa1180160:CDS 45.0%
GTCAGCATACAGAATAGGAG+AGG - chr8_1:13944045-13944064 None:intergenic 45.0%
GATGCGTCAGCATACAGAAT+AGG - chr8_1:13944050-13944069 None:intergenic 45.0%
CTGACGCATCATCTCCATAT+AGG + chr8_1:13944060-13944079 Msa1180160:intron 45.0%
!!! GCCTCTCATGTTTTAATGCG+AGG + chr8_1:13944669-13944688 Msa1180160:intron 45.0%
!! GGCCCAGTTTTAGTCTCTTA+AGG - chr8_1:13944795-13944814 None:intergenic 45.0%
GGCCGTCTTAACAAATTCGT+AGG - chr8_1:13944816-13944835 None:intergenic 45.0%
CTCACATGACTCTAGTGTGA+AGG + chr8_1:13945434-13945453 Msa1180160:CDS 45.0%
TGCAATCAGTCCAAAGATGC+AGG + chr8_1:13945469-13945488 Msa1180160:CDS 45.0%
TCGAGTGTTACCTGCATCTT+TGG - chr8_1:13945482-13945501 None:intergenic 45.0%
!! AAAGAAAATTATTAATAAAA+AGG - chr8_1:13944720-13944739 None:intergenic 5.0%
ACTTGCACTACAGGGTCACA+GGG - chr8_1:13941423-13941442 None:intergenic 50.0%
AACTTGCACTACAGGGTCAC+AGG - chr8_1:13941424-13941443 None:intergenic 50.0%
ACCCTGTAGTGCAAGTTCTG+AGG + chr8_1:13941427-13941446 Msa1180160:CDS 50.0%
AAGAAAGGAGCGAGAAGCCA+AGG - chr8_1:13941846-13941865 None:intergenic 50.0%
! GGTGATAACACTGGTTGGGA+AGG - chr8_1:13942642-13942661 None:intergenic 50.0%
! CTGGTGGTGATAACACTGGT+TGG - chr8_1:13942647-13942666 None:intergenic 50.0%
AGTATGAAGGGTCAGAGGGA+TGG + chr8_1:13943210-13943229 Msa1180160:intron 50.0%
TCATGTTGGTCACAGTGCTG+TGG + chr8_1:13944639-13944658 Msa1180160:intron 50.0%
CATGACTCTAGTGTGAAGGC+TGG + chr8_1:13945438-13945457 Msa1180160:CDS 50.0%
GTTTCTCCTGCAATGGCTGC+TGG + chr8_1:13941465-13941484 Msa1180160:CDS 55.0%
! GACGGAGGGAGTATGTTCCT+TGG + chr8_1:13941826-13941845 Msa1180160:intron 55.0%
! GCACCGGGCCTCAGATTTTT+GGG + chr8_1:13944544-13944563 Msa1180160:intron 55.0%
! CACCGGGCCTCAGATTTTTG+GGG + chr8_1:13944545-13944564 Msa1180160:intron 55.0%
!! ACCGGGCCTCAGATTTTTGG+GGG + chr8_1:13944546-13944565 Msa1180160:intron 55.0%
CTTGGGCCCCCAAAAATCTG+AGG - chr8_1:13944555-13944574 None:intergenic 55.0%
TGTTGGTCACAGTGCTGTGG+TGG + chr8_1:13944642-13944661 Msa1180160:intron 55.0%
!! GGCACCGGGCCTCAGATTTT+TGG + chr8_1:13944543-13944562 Msa1180160:intron 60.0%
! GCCCCCAAAAATCTGAGGCC+CGG - chr8_1:13944550-13944569 None:intergenic 60.0%
TGCCATAGCCCAGCCTCGAC+GGG - chr8_1:13944527-13944546 None:intergenic 65.0%
GTGCCATAGCCCAGCCTCGA+CGG - chr8_1:13944528-13944547 None:intergenic 65.0%
TCGAGGCTGGGCTATGGCAC+CGG + chr8_1:13944528-13944547 Msa1180160:intron 65.0%
! GTCACAGTGCTGTGGTGGCG+CGG + chr8_1:13944647-13944666 Msa1180160:intron 65.0%
!! AGCCTCGACGGGCTATGGGC+AGG - chr8_1:13944516-13944535 None:intergenic 70.0%
TGCCCATAGCCCGTCGAGGC+TGG + chr8_1:13944515-13944534 Msa1180160:intron 70.0%
GCCCATAGCCCGTCGAGGCT+GGG + chr8_1:13944516-13944535 Msa1180160:intron 70.0%
!! GCCCAGCCTCGACGGGCTAT+GGG - chr8_1:13944520-13944539 None:intergenic 70.0%
!! AGCCCAGCCTCGACGGGCTA+TGG - chr8_1:13944521-13944540 None:intergenic 70.0%
AGCCCGTCGAGGCTGGGCTA+TGG + chr8_1:13944522-13944541 Msa1180160:intron 70.0%
CGAGGCTGGGCTATGGCACC+GGG + chr8_1:13944529-13944548 Msa1180160:intron 70.0%
GGCCTGCCCATAGCCCGTCG+AGG + chr8_1:13944511-13944530 Msa1180160:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 13941395 13945682 13941395 ID=Msa1180160;Name=Msa1180160
chr8_1 mRNA 13941395 13945682 13941395 ID=Msa1180160-mRNA-1;Parent=Msa1180160;Name=Msa1180160-mRNA-1;_AED=0.13;_eAED=0.13;_QI=22|0.66|0.75|1|1|1|4|188|120
chr8_1 exon 13941395 13941486 13941395 ID=Msa1180160-mRNA-1:exon:5109;Parent=Msa1180160-mRNA-1
chr8_1 exon 13942597 13942679 13942597 ID=Msa1180160-mRNA-1:exon:5110;Parent=Msa1180160-mRNA-1
chr8_1 exon 13943917 13944005 13943917 ID=Msa1180160-mRNA-1:exon:5111;Parent=Msa1180160-mRNA-1
chr8_1 exon 13945374 13945682 13945374 ID=Msa1180160-mRNA-1:exon:5112;Parent=Msa1180160-mRNA-1
chr8_1 five_prime_UTR 13941395 13941416 13941395 ID=Msa1180160-mRNA-1:five_prime_utr;Parent=Msa1180160-mRNA-1
chr8_1 CDS 13941417 13941486 13941417 ID=Msa1180160-mRNA-1:cds;Parent=Msa1180160-mRNA-1
chr8_1 CDS 13942597 13942679 13942597 ID=Msa1180160-mRNA-1:cds;Parent=Msa1180160-mRNA-1
chr8_1 CDS 13943917 13944005 13943917 ID=Msa1180160-mRNA-1:cds;Parent=Msa1180160-mRNA-1
chr8_1 CDS 13945374 13945494 13945374 ID=Msa1180160-mRNA-1:cds;Parent=Msa1180160-mRNA-1
chr8_1 three_prime_UTR 13945495 13945682 13945495 ID=Msa1180160-mRNA-1:three_prime_utr;Parent=Msa1180160-mRNA-1
Gene Sequence

>Msa1180160

ATGCCCTGTGACCCTGTAGTGCAAGTTCTGAGGAAAGCTAATGTTCTCGTTTCTCCTGCAATGGCTGCTGGTGTAGGGATGAGTTGTCTACTTTTGGAATATTTTTATGGTTCCTTCCCAACCAGTGTTATCACCACCAGAATTGATTGTGAGGACAAATGTTTCGAAGTTGCAAAATGCATGATTGAAGCCATTAAGAGCAAAGGTGTTTCATCTAACGGGGCTGCTGAATTTTCTTTGTGTGTGAAGGTAAACCCTTGTTTTAGGGATTATTACCATTTTACTATGGAGTGCCAAGATTACTCACATGACTCTAGTGTGAAGGCTGGTATTTTGATGCAATCAGTCCAAAGATGCAGGTAA
Protein sequence

>Msa1180160

MPCDPVVQVLRKANVLVSPAMAAGVGMSCLLLEYFYGSFPTSVITTRIDCEDKCFEVAKCMIEAIKSKGVSSNGAAEFSLCVKVNPCFRDYYHFTMECQDYSHDSSVKAGILMQSVQRCR*