AlfalfaGEDB | Msa1180370

M. sativa cultivar zhongmu-4 / Msa1180370

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1180370	sp\|Q9LIG2\|RLK6_ARATH	48.696	345	169	4	14	353	558	899	4.48e-113	352

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1180370	A0A072TLD6	94.737	342	18	0	12	353	579	920	0.0	675

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1180370	Msa1182830	0.803161	3.978893e-49	-8.615850e-47
Msa1180370	Msa1274810	0.908501	1.595742e-81	-8.615850e-47
Msa1180370	Msa1312680	0.838751	2.386762e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1180370	MtrunA17_Chr8g0341111	92.669	341	25	0	12	352	444	784	659
Msa1180370	MtrunA17_Chr8g0341081	88.952	353	18	1	1	353	1	332	649
Msa1180370	MtrunA17_Chr8g0341151	81.269	331	62	0	23	353	535	865	563
Msa1180370	MtrunA17_Chr8g0340881	81.269	331	62	0	23	353	546	876	561
Msa1180370	MtrunA17_Chr8g0341101	78.378	333	72	0	21	353	590	922	548
Msa1180370	MtrunA17_Chr8g0340911	78.182	330	71	1	24	353	543	871	543
Msa1180370	MtrunA17_Chr8g0341011	74.203	345	89	0	9	353	531	875	542
Msa1180370	MtrunA17_Chr8g0340931	78.507	335	67	3	21	353	540	871	539
Msa1180370	MtrunA17_Chr8g0340871	80.000	320	62	2	35	353	563	881	526
Msa1180370	MtrunA17_Chr8g0347661	78.370	319	69	0	35	353	559	877	525

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1180370	AT1G51830.2	48.711	349	161	6	19	353	356	700	5.54e-115	349
Msa1180370	AT1G51830.1	48.711	349	161	6	19	353	349	693	7.27e-115	348
Msa1180370	AT2G28970.2	51.064	329	153	5	30	353	426	751	3.03e-114	348
Msa1180370	AT1G51820.2	50.000	330	155	5	30	353	298	623	3.10e-114	345
Msa1180370	AT3G21340.1	48.696	345	169	4	14	353	558	899	4.56e-114	352
Msa1180370	AT2G28970.1	51.064	329	153	5	30	353	461	786	8.61e-114	348
Msa1180370	AT4G29180.1	53.698	311	130	2	31	327	548	858	2.48e-113	350
Msa1180370	AT4G29180.2	53.698	311	130	2	31	327	550	860	2.93e-113	350
Msa1180370	AT4G29180.3	53.698	311	130	2	31	327	547	857	3.41e-113	350
Msa1180370	AT2G04300.1	48.094	341	166	5	21	353	514	851	1.58e-112	347

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Find 68 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAATAATTGGAGAAGG+AGG	0.545973	8_1:+14326641	Msa1180370:CDS
AAAATTCAGATATCTTAAAC+TGG	0.415513	8_1:+14327241	Msa1180370:CDS
AAACCATTCACATACTTCGG+TGG	0.558554	8_1:+14328074	Msa1180370:CDS
AAATCTCTATGCATAATAGG+TGG	0.620528	8_1:-14327406	None:intergenic
AAATGTACCAGCGGGGCGCG+TGG	0.571172	8_1:-14327527	None:intergenic
AACCATTCACATACTTCGGT+GGG	0.562397	8_1:+14328075	Msa1180370:CDS
AACCCACCGAAGTATGTGAA+TGG	0.550913	8_1:-14328077	None:intergenic
AACGTAACCAAATGTACCAG+CGG	0.670976	8_1:-14327536	None:intergenic
AAGAGATCATCGTCATGCAG+TGG	0.663853	8_1:-14328373	None:intergenic
AATATCTCCTCCTTTAACTA+AGG	0.322303	8_1:-14328104	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACAATAATTGGAGAAGG+AGG	+	chr8_1:14326641-14326660	Msa1180370:CDS	30.0%
GGATTTGGAAAAGTTTACAT+TGG	+	chr8_1:14326662-14326681	Msa1180370:CDS	30.0%
GATTGTTCATCATAGGAATT+TGG	+	chr8_1:14326910-14326929	Msa1180370:CDS	30.0%
TACATAGCACTTAATTGATG+TGG	+	chr8_1:14327346-14327365	Msa1180370:intron	30.0%
GATTGGATTATCTGCATAAT+GGG	+	chr8_1:14327377-14327396	Msa1180370:CDS	30.0%
AAATCTCTATGCATAATAGG+TGG	-	chr8_1:14327409-14327428	None:intergenic	30.0%
CATCTTTCTTATTGTTGCAA+AGG	+	chr8_1:14327726-14327745	Msa1180370:intron	30.0%
TCTTATTGTTGCAAAGGAAA+AGG	+	chr8_1:14327732-14327751	Msa1180370:intron	30.0%
ATGAAACCATTCACATACTT+CGG	+	chr8_1:14328071-14328090	Msa1180370:CDS	30.0%
ATATCTCCTCCTTTAACTAA+GGG	-	chr8_1:14328106-14328125	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	14326491	14328570	14326491	ID=Msa1180370;Name=Msa1180370
chr8_1	mRNA	14326491	14328570	14326491	ID=Msa1180370-mRNA-1;Parent=Msa1180370;Name=Msa1180370-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|1\|0.8\|1\|1\|1\|5\|198\|353
chr8_1	exon	14326491	14326766	14326491	ID=Msa1180370-mRNA-1:exon:5302;Parent=Msa1180370-mRNA-1
chr8_1	exon	14326896	14327022	14326896	ID=Msa1180370-mRNA-1:exon:5303;Parent=Msa1180370-mRNA-1
chr8_1	exon	14327238	14327300	14327238	ID=Msa1180370-mRNA-1:exon:5304;Parent=Msa1180370-mRNA-1
chr8_1	exon	14327377	14327566	14327377	ID=Msa1180370-mRNA-1:exon:5305;Parent=Msa1180370-mRNA-1
chr8_1	exon	14327967	14328570	14327967	ID=Msa1180370-mRNA-1:exon:5306;Parent=Msa1180370-mRNA-1
chr8_1	CDS	14326491	14326766	14326491	ID=Msa1180370-mRNA-1:cds;Parent=Msa1180370-mRNA-1
chr8_1	CDS	14326896	14327022	14326896	ID=Msa1180370-mRNA-1:cds;Parent=Msa1180370-mRNA-1
chr8_1	CDS	14327238	14327300	14327238	ID=Msa1180370-mRNA-1:cds;Parent=Msa1180370-mRNA-1
chr8_1	CDS	14327377	14327566	14327377	ID=Msa1180370-mRNA-1:cds;Parent=Msa1180370-mRNA-1
chr8_1	CDS	14327967	14328372	14327967	ID=Msa1180370-mRNA-1:cds;Parent=Msa1180370-mRNA-1
chr8_1	three_prime_UTR	14328373	14328570	14328373	ID=Msa1180370-mRNA-1:three_prime_utr;Parent=Msa1180370-mRNA-1

Gene Sequence

>Msa1180370

ATGTTTGAATATTTCTTCACGTGCTTTTGGTCAGCTTTATCATTTCATGTAGTTCCACCTGCACGTTCTAATTCTAAGAAGAGGGGTTCACTAAAATCAAAACATCAAGCATTCAGTTACACTGAAATTCTCAATATTACTGATAACTTCAAAACAATAATTGGAGAAGGAGGATTTGGAAAAGTTTACATTGGCATTTTACAAGATCGCACTCAAGTAGCTGTAAAAATGCTTTCAACTTCATCGAAGCAAGGTTATAAGGAATTTCAATCAGAGGTGCAACTTTTAATGATTGTTCATCATAGGAATTTGGTCTCTCTCATTGGATATTGTGATGAAGGTGAAATCAGAGCACTGATTTATGAGTACATGGCGAATGGAAATCTCCAACAATATTTATTAGTGGAAAATTCAGATATCTTAAACTGGACTAAGAGACTTAAAATTGCAGTTGATGCGGCACATGGATTGGATTATCTGCATAATGGGTGTAAACCACCTATTATGCATAGAGATTTGAAGTCTTCCAACATATTACTCGATGAGAACTTACGTGCGAAGATTGCTGATTTTGGTCTAAGTAGAGCTTTTGGTAATGATAATGATTCTCATATATCCACGCGCCCCGCTGGTACATTTGGTTACGTTGATCCACAATTTCAAAGAACAGGAAACTCAAATAAGAAAAATGATATCTACAGCTTTGGAATTATTCTATTTGAGTTGATCACTGGCAAGAAAGCACTAATAAAAGCACCTGATGAAACCATTCACATACTTCGGTGGGTTATTCCCTTAGTTAAAGGAGGAGATATTCAAAATGTTGTTGATACGAGGTTGAAAGGTGAATTCAACATAAATTCTGCATGGAAAGTTGTTGAGGTAGCCATGTCATGCATTTCACAAACTGCAGCTGATAGGCCAGACATTAGCCAAATACTAGTAGAGCTAAAGGAATGTCTATCTTTGGAGATAGTTCAGAGCAATAGTGGAAGCGCAAGAGATATAATTGAATTGACTTCTTTATCTACTGGATCAGAAATCACTCCGTCAGCTAGGTAA

Protein sequence

>Msa1180370

MFEYFFTCFWSALSFHVVPPARSNSKKRGSLKSKHQAFSYTEILNITDNFKTIIGEGGFGKVYIGILQDRTQVAVKMLSTSSKQGYKEFQSEVQLLMIVHHRNLVSLIGYCDEGEIRALIYEYMANGNLQQYLLVENSDILNWTKRLKIAVDAAHGLDYLHNGCKPPIMHRDLKSSNILLDENLRAKIADFGLSRAFGNDNDSHISTRPAGTFGYVDPQFQRTGNSNKKNDIYSFGIILFELITGKKALIKAPDETIHILRWVIPLVKGGDIQNVVDTRLKGEFNINSAWKVVEVAMSCISQTAADRPDISQILVELKECLSLEIVQSNSGSARDIIELTSLSTGSEITPSAR*