AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1182830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1182830 MtrunA17_Chr8g0338511 96.262 428 16 0 143 570 25 452 0.0 855
Msa1182830 MtrunA17_Chr8g0338511 47.283 184 47 3 13 146 11 194 7.29e-36 139
Msa1182830 MtrunA17_Chr8g0338471 79.856 417 79 2 146 561 23 435 0.0 697
Msa1182830 MtrunA17_Chr8g0338471 49.275 138 39 4 36 146 53 186 4.11e-24 107
Msa1182830 MtrunA17_Chr8g0338501 75.822 426 98 2 146 570 52 473 0.0 669
Msa1182830 MtrunA17_Chr8g0338501 47.101 138 42 4 36 146 82 215 4.89e-22 100
Msa1182830 MtrunA17_Chr8g0338521 71.148 357 92 5 165 518 12 360 1.21e-172 493
Msa1182830 MtrunA17_Chr8g0338521 59.055 127 24 4 48 146 35 161 8.77e-30 120
Msa1182830 MtrunA17_Chr8g0338531 67.324 355 93 4 211 558 8 346 3.20e-156 465
Msa1182830 MtrunA17_Chr8g0338531 53.191 94 37 1 60 146 16 109 2.08e-19 92.4
Msa1182830 MtrunA17_Chr2g0311541 44.153 419 229 5 151 566 18 434 2.10e-118 369
Msa1182830 MtrunA17_Chr2g0311551 44.578 415 225 5 151 564 259 669 7.91e-118 373
Msa1182830 MtrunA17_Chr8g0338481 67.782 239 52 1 316 554 1 214 3.82e-112 335
Msa1182830 MtrunA17_Chr2g0311521 43.645 417 230 5 152 566 261 674 5.45e-112 357
Msa1182830 MtrunA17_Chr5g0399591 41.889 413 214 12 169 565 40 442 2.17e-101 324
Msa1182830 MtrunA17_Chr5g0399561 39.486 428 232 13 159 570 32 448 2.14e-95 308
Msa1182830 MtrunA17_Chr2g0311511 42.769 325 174 8 151 465 35 357 1.34e-77 259
Msa1182830 MtrunA17_Chr5g0394381 32.944 428 248 19 152 569 24 422 1.73e-46 174
Msa1182830 MtrunA17_Chr4g0063701 30.488 410 243 17 165 555 51 437 7.17e-39 152
Msa1182830 MtrunA17_Chr4g0063691 30.657 411 245 17 152 541 37 428 5.62e-33 134
Msa1182830 MtrunA17_Chr3g0106201 27.857 420 267 12 157 563 24 420 5.41e-32 131
Msa1182830 MtrunA17_Chr0c04g0492001 28.235 425 271 16 155 567 29 431 6.51e-32 131
Msa1182830 MtrunA17_Chr3g0134291 28.235 425 271 16 155 567 29 431 2.00e-31 129
Msa1182830 MtrunA17_Chr0c04g0492031 28.235 425 271 16 155 567 29 431 2.23e-31 129
Msa1182830 MtrunA17_Chr3g0106141 28.369 423 270 13 157 566 24 426 7.60e-30 125
Msa1182830 MtrunA17_Chr3g0106151 28.814 413 241 15 168 563 38 414 8.19e-30 124
Msa1182830 MtrunA17_Chr3g0106121 28.502 414 261 12 157 557 24 415 1.80e-29 124
Msa1182830 MtrunA17_Chr3g0106131 28.605 430 264 12 157 570 24 426 5.52e-29 122
Msa1182830 MtrunA17_Chr3g0106211 29.111 371 236 12 157 519 27 378 1.64e-27 117
Msa1182830 MtrunA17_Chr3g0106221 27.882 373 225 10 168 520 37 385 5.63e-27 116
Msa1182830 MtrunA17_Chr7g0257821 28.070 399 243 16 169 558 45 408 4.15e-26 113
Msa1182830 MtrunA17_Chr1g0202331 25.556 450 274 19 150 560 24 451 2.79e-25 110
Msa1182830 MtrunA17_Chr3g0106191 29.155 367 218 13 206 563 2 335 4.44e-22 100
Msa1182830 MtrunA17_Chr7g0237341 25.799 407 259 16 168 559 43 421 5.60e-20 94.4
Msa1182830 MtrunA17_Chr7g0237351 24.215 446 293 17 142 570 17 434 6.09e-20 94.0
Msa1182830 MtrunA17_Chr2g0323301 26.368 402 233 17 150 520 22 391 8.46e-20 93.6
Msa1182830 MtrunA17_Chr4g0042391 26.171 363 232 12 168 519 40 377 1.18e-19 93.2
Msa1182830 MtrunA17_Chr4g0053161 24.709 429 273 17 150 559 44 441 5.41e-19 91.3
Msa1182830 MtrunA17_Chr7g0237331 24.384 406 262 12 168 557 44 420 9.52e-19 90.5
Msa1182830 MtrunA17_Chr4g0042351 25.895 363 229 14 170 519 42 377 3.31e-18 88.6
Msa1182830 MtrunA17_Chr3g0137741 26.902 368 220 17 167 525 52 379 4.09e-17 85.1
Msa1182830 MtrunA17_Chr8g0361311 27.513 378 216 17 168 520 39 383 8.94e-17 83.2
Msa1182830 MtrunA17_Chr4g0023071 23.896 385 236 14 168 524 42 397 1.03e-16 84.0
Msa1182830 MtrunA17_Chr2g0280701 26.053 380 219 18 168 520 38 382 1.68e-16 83.2
Msa1182830 MtrunA17_Chr8g0361251 27.249 378 217 17 168 520 39 383 3.46e-16 82.4
Msa1182830 MtrunA17_Chr8g0361301 26.075 372 228 14 168 520 40 383 3.49e-16 82.4
Msa1182830 MtrunA17_Chr4g0042381 25.137 366 233 13 168 519 39 377 4.18e-16 82.0
Msa1182830 MtrunA17_Chr3g0084621 25.397 378 224 17 168 521 44 387 1.07e-15 80.5
Msa1182830 MtrunA17_Chr2g0280681 25.858 379 216 18 168 519 43 383 1.10e-15 80.5
Msa1182830 MtrunA17_Chr8g0361261 25.060 419 266 15 122 520 24 414 2.10e-15 79.7
Msa1182830 MtrunA17_Chr3g0134321 33.553 152 94 4 168 314 38 187 2.70e-15 79.3
Msa1182830 MtrunA17_Chr3g0098791 23.457 405 251 15 138 520 18 385 3.36e-15 78.6
Msa1182830 MtrunA17_Chr2g0280801 23.409 440 266 18 169 569 45 452 6.00e-15 78.2
Msa1182830 MtrunA17_Chr2g0318811 26.768 396 232 17 146 520 20 378 1.88e-14 76.6
Msa1182830 MtrunA17_Chr4g0048691 27.692 390 228 20 168 529 61 424 1.89e-14 76.3
Msa1182830 MtrunA17_Chr8g0361441 26.633 398 253 15 138 520 14 387 1.93e-14 76.6
Msa1182830 MtrunA17_Chr5g0428111 26.289 388 242 18 156 524 31 393 2.12e-14 76.6
Msa1182830 MtrunA17_Chr2g0280731 25.984 381 233 15 168 530 46 395 3.11e-14 75.9
Msa1182830 MtrunA17_Chr4g0048791 25.606 371 238 13 168 520 55 405 3.56e-14 75.9
Msa1182830 MtrunA17_Chr7g0236201 22.970 431 264 18 168 560 44 444 4.80e-14 75.5
Msa1182830 MtrunA17_Chr7g0236161 22.917 432 263 18 168 560 44 444 5.01e-14 75.1
Msa1182830 MtrunA17_Chr3g0084411 23.919 393 232 15 168 530 41 396 6.42e-14 75.1
Msa1182830 MtrunA17_Chr3g0084491 25.135 370 232 14 168 520 57 398 6.89e-14 75.1
Msa1182830 MtrunA17_Chr2g0280841 23.741 417 249 20 175 554 53 437 9.09e-14 73.6
Msa1182830 MtrunA17_Chr3g0093161 24.339 378 251 14 156 520 25 380 1.03e-13 74.3
Msa1182830 MtrunA17_Chr4g0042421 24.619 394 247 17 149 519 19 385 1.28e-13 73.9
Msa1182830 MtrunA17_Chr7g0236121 22.759 435 272 17 168 568 44 448 1.60e-13 73.6
Msa1182830 MtrunA17_Chr1g0149381 26.077 418 240 21 167 565 57 424 1.85e-13 73.6
Msa1182830 MtrunA17_Chr3g0084681 24.282 383 220 16 168 520 44 386 2.01e-13 73.6
Msa1182830 MtrunA17_Chr3g0078521 27.792 385 216 22 168 519 40 395 2.64e-13 73.2
Msa1182830 MtrunA17_Chr2g0318831 26.471 374 221 17 168 520 41 381 4.31e-13 72.4
Msa1182830 MtrunA17_Chr3g0084651 24.074 378 228 15 168 519 41 385 4.52e-13 71.6
Msa1182830 MtrunA17_Chr2g0318841 24.599 374 228 16 168 520 38 378 6.36e-13 71.6
Msa1182830 MtrunA17_Chr2g0280791 24.409 381 237 15 175 530 47 401 8.00e-13 71.6
Msa1182830 MtrunA17_Chr2g0318171 25.114 438 262 20 145 564 30 419 9.79e-13 71.2
Msa1182830 MtrunA17_Chr3g0084431 23.573 403 231 16 151 520 22 380 1.31e-12 70.9
Msa1182830 MtrunA17_Chr1g0205551 29.932 147 89 6 250 391 1 138 1.74e-12 65.1
Msa1182830 MtrunA17_Chr2g0333091 25.990 404 242 17 167 555 50 411 2.35e-12 70.1
Msa1182830 MtrunA17_Chr8g0340091 31.361 169 101 7 153 309 80 245 2.40e-12 70.1
Msa1182830 MtrunA17_Chr4g0048651 26.433 314 187 14 168 459 61 352 2.44e-12 69.3
Msa1182830 MtrunA17_Chr3g0078531 28.052 385 215 21 168 519 52 407 2.60e-12 69.7
Msa1182830 MtrunA17_Chr4g0048761 25.323 387 219 19 168 520 56 406 3.52e-12 69.3
Msa1182830 MtrunA17_Chr3g0106161 30.055 183 106 5 382 559 90 255 4.13e-12 68.9
Msa1182830 MtrunA17_Chr2g0323241 30.556 180 114 5 150 320 23 200 4.68e-12 68.6
Msa1182830 MtrunA17_Chr4g0042361 24.804 383 226 18 168 520 74 424 4.93e-12 68.9
Msa1182830 MtrunA17_Chr4g0048641 30.314 287 155 17 168 432 63 326 5.12e-12 68.9
Msa1182830 MtrunA17_Chr3g0084451 24.011 379 223 14 168 520 44 383 5.90e-12 68.6
Msa1182830 MtrunA17_Chr8g0340061 31.361 169 101 7 153 309 27 192 8.65e-12 67.8
Msa1182830 MtrunA17_Chr7g0236641 23.272 434 270 21 152 553 25 427 9.86e-12 68.2
Msa1182830 MtrunA17_Chr7g0237391 23.039 408 270 16 168 559 42 421 1.09e-11 67.8
Msa1182830 MtrunA17_Chr8g0361431 25.749 334 210 13 150 472 34 340 2.30e-11 66.6
Msa1182830 MtrunA17_Chr4g0048801 42.063 126 54 6 170 280 39 160 4.56e-11 65.9
Msa1182830 MtrunA17_Chr3g0078541 27.487 382 220 22 168 519 52 406 5.13e-11 65.9
Msa1182830 MtrunA17_Chr8g0340071 29.139 151 96 4 168 309 44 192 5.82e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1182830 AT5G24080.2 27.211 441 270 20 148 556 26 447 1.69e-28 121
Msa1182830 AT2G41890.1 27.604 384 242 14 150 524 24 380 5.95e-18 88.2
Msa1182830 AT1G65790.3 24.682 393 239 13 150 521 28 384 1.45e-17 86.7
Msa1182830 AT1G65790.2 24.682 393 239 13 150 521 28 384 1.45e-17 86.7
Msa1182830 AT1G34300.1 25.191 393 244 17 150 533 25 376 1.70e-17 87.0
Msa1182830 AT1G65790.1 25.255 392 236 13 150 520 28 383 1.70e-17 87.0
Msa1182830 AT4G21390.2 25.653 421 258 16 141 535 18 409 4.10e-17 85.5
Msa1182830 AT4G21390.1 25.653 421 258 16 141 535 18 409 4.34e-17 85.5
Msa1182830 AT3G12000.1 26.302 384 236 14 159 520 40 398 6.22e-17 84.0
Msa1182830 AT1G11340.1 26.893 383 212 18 168 519 34 379 5.01e-16 82.0
Msa1182830 AT1G11340.2 26.893 383 212 18 168 519 102 447 1.11e-15 80.9
Msa1182830 AT1G11340.3 26.893 383 212 18 168 519 102 447 1.16e-15 80.9
Msa1182830 AT1G11330.3 25.641 390 247 15 163 530 42 410 1.32e-15 80.9
Msa1182830 AT1G11330.2 25.641 390 247 15 163 530 42 410 1.39e-15 80.9
Msa1182830 AT1G11330.1 25.641 390 247 15 163 530 42 410 1.40e-15 80.9
Msa1182830 AT1G11410.3 24.865 370 236 16 168 519 13 358 5.52e-15 79.0
Msa1182830 AT1G11410.1 24.865 370 236 16 168 519 37 382 5.86e-15 79.0
Msa1182830 AT1G11410.4 24.865 370 236 16 168 519 44 389 6.15e-15 78.6
Msa1182830 AT1G11280.2 23.457 405 232 16 168 535 42 405 1.04e-14 77.8
Msa1182830 AT1G11280.5 23.457 405 232 16 168 535 42 405 1.09e-14 77.8
Msa1182830 AT1G11280.4 24.510 408 224 17 168 535 52 415 1.10e-14 77.8
Msa1182830 AT1G11280.3 23.457 405 232 16 168 535 42 405 1.19e-14 77.8
Msa1182830 AT1G11280.1 24.510 408 224 17 168 535 52 415 1.38e-14 77.4
Msa1182830 AT4G21380.2 22.959 392 255 13 148 521 28 390 1.55e-14 77.0
Msa1182830 AT4G21380.1 22.506 391 256 13 148 520 28 389 3.00e-14 76.6
Msa1182830 AT1G11300.2 24.623 398 246 18 158 530 32 400 6.94e-14 75.5
Msa1182830 AT1G11300.1 24.623 398 246 18 158 530 32 400 8.71e-14 75.1
Msa1182830 AT1G11303.1 24.760 416 235 17 158 535 32 407 2.13e-13 73.9
Msa1182830 AT1G65800.1 25.000 384 227 17 163 520 39 387 2.24e-13 73.6
Msa1182830 AT1G65800.2 24.935 385 228 17 163 521 39 388 2.45e-13 73.2
Msa1182830 AT1G78860.1 23.280 378 247 16 178 531 52 410 5.94e-13 71.6
Msa1182830 AT1G61380.1 23.664 393 247 17 168 535 35 399 6.38e-13 72.4
Msa1182830 AT1G78850.1 23.077 377 249 15 178 531 52 410 1.37e-12 70.5
Msa1182830 AT1G16905.1 24.054 370 217 15 186 531 69 398 2.46e-12 69.7
Msa1182830 AT1G11350.1 25.238 420 221 20 157 530 26 398 8.25e-12 68.6
Msa1182830 AT1G61370.1 24.455 413 247 18 157 535 23 404 8.31e-12 68.6
Msa1182830 AT4G03230.8 23.174 397 249 17 190 562 68 432 1.90e-11 67.4
Msa1182830 AT4G03230.4 23.185 427 271 18 168 569 44 438 2.65e-11 67.0
Msa1182830 AT4G03230.1 23.185 427 271 18 168 569 44 438 2.65e-11 67.0
Msa1182830 AT1G61400.1 23.907 389 245 14 168 535 48 406 3.52e-11 66.6
Msa1182830 AT1G61400.2 23.907 389 245 14 168 535 48 406 4.66e-11 66.2
Msa1182830 AT5G24080.3 24.629 337 206 17 251 556 1 320 7.46e-11 65.5
Msa1182830 AT5G24080.1 24.629 337 206 17 251 556 1 320 7.46e-11 65.5

Find 123 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCACCGCGCATTGGTTTC+TGG 0.137963 8_1:-18001699 Msa1182830:intron
CCATGGTTGCTTTCACCTTC+TGG 0.162410 8_1:-17998984 Msa1182830:CDS
CTACACTGCTCTTCAGTAAA+AGG 0.260924 8_1:+17997973 None:intergenic
ATGGTGAACTTAACAGTCTT+TGG 0.262148 8_1:-18000900 Msa1182830:CDS
ACCAATGGCTATGGTACTTT+TGG 0.266017 8_1:+17999037 None:intergenic
AGTATAAGTACATATATTAA+AGG 0.280779 8_1:+17998242 None:intergenic
CCTTCTGGTGACTTTGCCTT+TGG 0.284286 8_1:-17998969 Msa1182830:CDS
CAGCTTCCATATGGATCATT+AGG 0.288789 8_1:+17998117 None:intergenic
TCAATCAGTGAGTAATGTTT+TGG 0.296727 8_1:+17998141 None:intergenic
CCTTCTCAAGTTGTGGATAA+AGG 0.297314 8_1:-17998642 Msa1182830:CDS
CACTGGCAACTTTGTGCTTC+AGG 0.298549 8_1:-17998721 Msa1182830:CDS
GCTGCCACTCCTGCTGCCTT+TGG 0.312421 8_1:+18001736 None:intergenic
AAATCTTTCTTCAAGGCTTA+AGG 0.312957 8_1:-17998616 Msa1182830:CDS
TCACTCTTAGTTGAACTCTT+AGG 0.318025 8_1:+17998291 None:intergenic
CTCCAAATGGTGTCAGGTTA+TGG 0.329745 8_1:-17998791 Msa1182830:CDS
TATAATAGCTTCTGCACTTT+GGG 0.334674 8_1:-17998192 Msa1182830:CDS
GCACATCCTTGGATGAAATC+TGG 0.356014 8_1:+17998090 None:intergenic
TTCCATAGCATGTCACCATT+TGG 0.368612 8_1:+18000991 None:intergenic
TTCCATAACCTGACACCATT+TGG 0.373337 8_1:+17998789 None:intergenic
TGACAGGTATGGTGGAACTA+TGG 0.376095 8_1:+17997797 None:intergenic
AAGGAAGAAATCTTTCTTCA+AGG 0.378244 8_1:-17998623 Msa1182830:CDS
CACAACTTGAGAAGGCAATA+AGG 0.378374 8_1:+17998650 None:intergenic
GATCATCTTCTGCACATCCT+TGG 0.382950 8_1:+17998079 None:intergenic
CATTCTATTAATTTACCATC+TGG 0.385953 8_1:-17998531 Msa1182830:CDS
TGACGCCTTCTTGCCTTCTC+AGG 0.401906 8_1:-18000857 Msa1182830:CDS
AAGAAGTTGCCACTTTCAAA+TGG 0.408488 8_1:-17997883 Msa1182830:CDS
TGGTGAACTTAACAGTCTTT+GGG 0.415246 8_1:-18000899 Msa1182830:CDS
GAGAAACTCACTGCCAATGA+TGG 0.416886 8_1:-18001030 Msa1182830:CDS
ATCAAGTGCTGGAGCTCAAT+TGG 0.420184 8_1:-17998460 Msa1182830:CDS
AGAGTTGATGCAACTCTTAA+TGG 0.425621 8_1:-17997859 Msa1182830:CDS
AGCTCCAGCACTTGATGTAC+TGG 0.427066 8_1:+17998467 None:intergenic
CTCCAAATGGTGACATGCTA+TGG 0.432332 8_1:-18000993 Msa1182830:CDS
TCAGGTTAGGTGATAGTTGT+TGG 0.437474 8_1:-17997909 Msa1182830:CDS
TCTGGCTTGCAGCTTCCATA+TGG 0.442901 8_1:+17998108 None:intergenic
TGGACGGCTGTTGGCAGCGT+TGG 0.442949 8_1:-18001770 Msa1182830:CDS
ATTGATCCTAATGATCCATA+TGG 0.445850 8_1:-17998123 Msa1182830:CDS
CAATTGGTCTTTGACAAGTC+AGG 0.449002 8_1:-17998444 Msa1182830:CDS
ACTCTATCACCAAGTCCCTC+AGG 0.451951 8_1:+18000967 None:intergenic
AAAGAGACTAATTTCAGTAA+AGG 0.455417 8_1:-18000820 Msa1182830:CDS
CTTATTGCCTTCTCAAGTTG+TGG 0.458043 8_1:-17998649 Msa1182830:CDS
ACTGTTGTGTGGTATGCTAA+TGG 0.458658 8_1:-17998879 Msa1182830:CDS
AGCTAGCACCAGCCCATCAT+TGG 0.459051 8_1:+18001017 None:intergenic
GTGAAGATGGCCAGTGGCGT+AGG 0.459420 8_1:-18001796 None:intergenic
AGAAATGTTTGCATACCAAA+TGG 0.463887 8_1:+18001053 None:intergenic
TGCCTTCTCAGGTTGTTGAA+AGG 0.468344 8_1:-18000846 Msa1182830:CDS
GTGAAGGTTGGACCACAGTA+TGG 0.468347 8_1:-17998272 Msa1182830:CDS
CTATGGAACCCTGAGGGACT+TGG 0.471465 8_1:-18000976 Msa1182830:CDS
GTGGAACTCACTGCTAATGA+TGG 0.472800 8_1:-17998828 Msa1182830:CDS
CTGGTGCTAGCTTCTCCAAA+TGG 0.475850 8_1:-18001006 Msa1182830:CDS
ATTTAATACTAATTTGTGAC+AGG 0.481714 8_1:+17997781 None:intergenic
AAGGAGACAGATTTCAGTAA+AGG 0.482797 8_1:-17998597 Msa1182830:CDS
ATATAATAGCTTCTGCACTT+TGG 0.487312 8_1:-17998193 Msa1182830:CDS
GTATAAGTACATATATTAAA+GGG 0.488197 8_1:+17998243 None:intergenic
CCGTCCATCCCCTACGCCAC+TGG 0.488509 8_1:+18001786 None:intergenic
CCTTTATCCACAACTTGAGA+AGG 0.492964 8_1:+17998642 None:intergenic
GCAGTGTAGGAAATCTTGTA+TGG 0.504417 8_1:-17997959 Msa1182830:CDS
TAACTTCTCCAAATGGTGTC+AGG 0.504860 8_1:-17998797 Msa1182830:CDS
ATACTAATTTGTGACAGGTA+TGG 0.507981 8_1:+17997786 None:intergenic
GGATGATTGTTTGTGTTCTG+TGG 0.510059 8_1:-17997938 Msa1182830:CDS
GGAGACACTCCTGCACCAAA+AGG 0.513474 8_1:-17998858 Msa1182830:CDS
CCAGTGGCGTAGGGGATGGA+CGG 0.513783 8_1:-18001786 Msa1182830:CDS
CAGCAGGAGTGGCAGCAAGA+AGG 0.514132 8_1:-18001729 Msa1182830:CDS
TTGGTGCTAACTTCTCCAAA+TGG 0.515119 8_1:-17998804 Msa1182830:CDS
TGAAGATGGCCAGTGGCGTA+GGG 0.515901 8_1:-18001795 None:intergenic
AAAAGAATCACCAATGGCTA+TGG 0.516781 8_1:+17999028 None:intergenic
AGTGCAGGTAGTGGTGTTTG+TGG 0.518931 8_1:-17998216 Msa1182830:CDS
AGAAGTTGCCACTTTCAAAT+GGG 0.520202 8_1:-17997882 Msa1182830:CDS
TATCAGATTATGTGCTTCAA+AGG 0.523234 8_1:-17997996 Msa1182830:CDS
TGCAACTCTTAATGGTGCTA+AGG 0.523300 8_1:-17997851 Msa1182830:CDS
ATGGCCAGTGGCGTAGGGGA+TGG 0.523578 8_1:-18001790 Msa1182830:CDS
ACTCACTGCCAATGATGGGC+TGG 0.527611 8_1:-18001025 Msa1182830:CDS
TGTGGTGTGTTCAATGACAT+TGG 0.533191 8_1:-18000940 Msa1182830:CDS
AGCCTTTCAACAACCTGAGA+AGG 0.536142 8_1:+18000844 None:intergenic
TGAAGCACATAATCTGATAG+AGG 0.536988 8_1:+17998000 None:intergenic
CATTGGAAACTCTGTGCTTG+AGG 0.537589 8_1:-18000923 Msa1182830:CDS
CAAGATGACTCACCGCGCAT+TGG 0.541030 8_1:-18001706 Msa1182830:CDS
AGACAGATTTCAGTAAAGGA+AGG 0.543432 8_1:-17998593 Msa1182830:CDS
GAGATCCTTCTACAAAATGA+TGG 0.546683 8_1:-18000790 Msa1182830:intron
AAATCCAGTACATCAAGTGC+TGG 0.547246 8_1:-17998471 Msa1182830:CDS
GACAGGTATGGTGGAACTAT+GGG 0.550857 8_1:+17997798 None:intergenic
TCAACTCTCCCATTTGAAAG+TGG 0.553723 8_1:+17997874 None:intergenic
TGCACCAAAAGGATCAAAAG+TGG 0.560768 8_1:-17998847 Msa1182830:CDS
CTCAATGTTAAAGTTTCTCA+CGG 0.563629 8_1:-17998756 Msa1182830:CDS
ACCAAAAGTACCATAGCCAT+TGG 0.565235 8_1:-17999038 Msa1182830:CDS
TATACTGTTAGTGCAGGTAG+TGG 0.565930 8_1:-17998225 Msa1182830:CDS
AAAAGATTAGTGTCTTTGAG+TGG 0.569176 8_1:+17998939 None:intergenic
AAGCAACCATGGAGATGTGC+TGG 0.569700 8_1:+17998995 None:intergenic
ATGGTAAATTCAACAGTCTG+TGG 0.569947 8_1:-17998698 Msa1182830:CDS
ACTCACTGCTAATGATGGGT+TGG 0.571054 8_1:-17998823 Msa1182830:CDS
AATTGGTCTTTGACAAGTCA+GGG 0.572130 8_1:-17998443 Msa1182830:CDS
AGAAACTCACTGCCAATGAT+GGG 0.573388 8_1:-18001029 Msa1182830:CDS
TGCAAGCCAGATTTCATCCA+AGG 0.573649 8_1:-17998096 Msa1182830:CDS
GGAAACTCTGTGCTTGAGGA+TGG 0.575358 8_1:-18000919 Msa1182830:CDS
GGCAACTTTGTGCTTCAGGA+TGG 0.577553 8_1:-17998717 Msa1182830:CDS
TGTACTTATACTGTTAGTGC+AGG 0.578443 8_1:-17998231 Msa1182830:CDS
CTCTATCACCAAGTCCCTCA+GGG 0.584919 8_1:+18000968 None:intergenic
CTAATTTGTGACAGGTATGG+TGG 0.587677 8_1:+17997789 None:intergenic
TGGTAAATTCAACAGTCTGT+GGG 0.591832 8_1:-17998697 Msa1182830:CDS
ACATCCAAAGGCAGCAGGAG+TGG 0.592089 8_1:-18001740 Msa1182830:CDS
CTTGGTGATAGAGTTTCTTG+TGG 0.592795 8_1:-18000958 Msa1182830:CDS
AGACTAATTTCAGTAAAGGA+AGG 0.594962 8_1:-18000816 Msa1182830:CDS
CACAAGCCAGCACATCTCCA+TGG 0.597254 8_1:-17999001 Msa1182830:CDS
CCAGAAGGTGAAAGCAACCA+TGG 0.600409 8_1:+17998984 None:intergenic
GAAGGCAATAAGGTGTCACT+AGG 0.602021 8_1:+17998660 None:intergenic
AAGAGTTCAACTAAGAGTGA+AGG 0.613595 8_1:-17998288 Msa1182830:CDS
AACAACCTGAGAAGGCAAGA+AGG 0.614766 8_1:+18000852 None:intergenic
GTTCAACTAAGAGTGAAGGT+TGG 0.619862 8_1:-17998284 Msa1182830:CDS
GTTGGAACGACGACATCCAA+AGG 0.626355 8_1:-18001752 Msa1182830:CDS
TGCAGTAAAAGAATCACCAA+TGG 0.629150 8_1:+17999022 None:intergenic
GTCAGGTTATGGAATACTGA+AGG 0.629411 8_1:-17998780 Msa1182830:CDS
CCAAAGGCAAAGTCACCAGA+AGG 0.636933 8_1:+17998969 None:intergenic
CACGGTGTGTTGAATGACAC+TGG 0.640680 8_1:-17998738 Msa1182830:CDS
TGGAACTCACTGCTAATGAT+GGG 0.649428 8_1:-17998827 Msa1182830:CDS
TTTGAGTGGAAGAAAACCAA+AGG 0.654690 8_1:+17998953 None:intergenic
AGGTATGGTGGAACTATGGG+AGG 0.656973 8_1:+17997801 None:intergenic
TGCTCTAAGATAAAATTGTG+TGG 0.657315 8_1:+17998353 None:intergenic
GAAGATGGCCAGTGGCGTAG+GGG 0.662272 8_1:-18001794 None:intergenic
AAAGTGGTGAAGATGGCCAG+TGG 0.688073 8_1:-18001802 None:intergenic
TTTCTGAGAAAACTGTTGTG+TGG 0.694901 8_1:-17998890 Msa1182830:CDS
GACATGCTATGGAACCCTGA+GGG 0.702953 8_1:-18000982 Msa1182830:CDS
ACAAACCAGAAACCAATGCG+CGG 0.732614 8_1:+18001694 None:intergenic
AAAGGGCTTAGACCATACTG+TGG 0.757626 8_1:+17998260 None:intergenic
TGACATGCTATGGAACCCTG+AGG 0.779667 8_1:-18000983 Msa1182830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATTTAAAAAAAAAA+AGG - chr8_1:17998108-17998127 Msa1182830:CDS 0.0%
!!! TTTTTTGTTAATTTAACATT+TGG - chr8_1:17998825-17998844 Msa1182830:CDS 10.0%
!!! ATTTTAATTTTCTATGAAAA+AGG - chr8_1:18000217-18000236 Msa1182830:intron 10.0%
!!! TTTTAATTTTCTATGAAAAA+GGG - chr8_1:18000218-18000237 Msa1182830:intron 10.0%
!! AAAAATATCTATAAATCATA+TGG - chr8_1:18000344-18000363 Msa1182830:intron 10.0%
!! ATATGATAATATCTGTAAAA+GGG + chr8_1:17999598-17999617 None:intergenic 15.0%
!! TATATGATAATATCTGTAAA+AGG + chr8_1:17999599-17999618 None:intergenic 15.0%
!! TTATTTCCTATAAAAATCAT+AGG - chr8_1:18000423-18000442 Msa1182830:intron 15.0%
!! TATTTCCTATAAAAATCATA+GGG - chr8_1:18000424-18000443 Msa1182830:intron 15.0%
!! GTATAAGTACATATATTAAA+GGG + chr8_1:18001339-18001358 None:intergenic 15.0%
!! AGTATAAGTACATATATTAA+AGG + chr8_1:18001340-18001359 None:intergenic 15.0%
!! AAATGAGAACAATATTTGTT+CGG - chr8_1:17997969-17997988 Msa1182830:CDS 20.0%
!! TCTATTTACTATGAATCATA+TGG - chr8_1:17998280-17998299 Msa1182830:CDS 20.0%
!! TTTAGATTATAGAACCAATT+CGG + chr8_1:17998307-17998326 None:intergenic 20.0%
!!! ATCAACTTTTTAACTATCTT+AGG + chr8_1:17999921-17999940 None:intergenic 20.0%
!!! ATCTTTTTTTGCTTTCAATT+TGG - chr8_1:18000656-18000675 Msa1182830:intron 20.0%
! AACAACATAAATCCGTAAAA+GGG + chr8_1:17997911-17997930 None:intergenic 25.0%
! AAACAACATAAATCCGTAAA+AGG + chr8_1:17997912-17997931 None:intergenic 25.0%
!!! TTGGTTCTATAATCTAAACA+TGG - chr8_1:17998309-17998328 Msa1182830:CDS 25.0%
! AAATGGAAAGCAAAAAAACA+TGG + chr8_1:17998512-17998531 None:intergenic 25.0%
!!! ATGTTTTTTTGCTTTCCATT+TGG - chr8_1:17998511-17998530 Msa1182830:CDS 25.0%
! AAAGAGACTAATTTCAGTAA+AGG - chr8_1:17998759-17998778 Msa1182830:CDS 25.0%
! TAACAGACAAAATTAGTGAA+CGG - chr8_1:17999690-17999709 Msa1182830:intron 25.0%
!! TGATTATTGAATACGTTTTC+AGG + chr8_1:17999834-17999853 None:intergenic 25.0%
!!! TCTTAGGTTATAGTTTTACA+GGG + chr8_1:17999905-17999924 None:intergenic 25.0%
!!! ATCTTAGGTTATAGTTTTAC+AGG + chr8_1:17999906-17999925 None:intergenic 25.0%
!!! AAAGTTGATAACAAATAGAC+AGG - chr8_1:17999932-17999951 Msa1182830:intron 25.0%
!!! ATTGAAGTTGATGTTAGTAT+CGG + chr8_1:18000008-18000027 None:intergenic 25.0%
!!! ATACATTACAATTTTGACCA+TGG + chr8_1:18000193-18000212 None:intergenic 25.0%
! TCATACTAAATCAACAAACA+AGG + chr8_1:18000282-18000301 None:intergenic 25.0%
!! TTTAGTATGAGTTGACTATA+AGG - chr8_1:18000292-18000311 Msa1182830:intron 25.0%
!!! ACTAAGTGCTTTAATTTTGA+TGG - chr8_1:17998246-17998265 Msa1182830:CDS 25.0%
! ATAAATCATATGGACCAAAT+TGG - chr8_1:18000354-18000373 Msa1182830:intron 25.0%
!! TTGATCCCTATGATTTTTAT+AGG + chr8_1:18000432-18000451 None:intergenic 25.0%
!! ACAGTTTTCTCAGAAATTTT+GGG + chr8_1:18000685-18000704 None:intergenic 25.0%
!! AACAGTTTTCTCAGAAATTT+TGG + chr8_1:18000686-18000705 None:intergenic 25.0%
! CATTCTATTAATTTACCATC+TGG - chr8_1:18001048-18001067 Msa1182830:CDS 25.0%
!! TTTTCATTAACATAACCAGA+TGG + chr8_1:18001066-18001085 None:intergenic 25.0%
!! AGATCATTTCTCTTTTTACT+TGG + chr8_1:18001525-18001544 None:intergenic 25.0%
! GTATTTGTGTTCTTCTAAAG+TGG + chr8_1:17998070-17998089 None:intergenic 30.0%
ATTGAAAAATCAGTGAGTAG+AGG + chr8_1:17998357-17998376 None:intergenic 30.0%
! TCTTCTAGTAACTTTTCGTT+AGG - chr8_1:17998466-17998485 Msa1182830:CDS 30.0%
AGAAATGTTTGCATACCAAA+TGG + chr8_1:17998529-17998548 None:intergenic 30.0%
AGACTAATTTCAGTAAAGGA+AGG - chr8_1:17998763-17998782 Msa1182830:CDS 30.0%
! GATTACCATCATTTTGTAGA+AGG + chr8_1:17998797-17998816 None:intergenic 30.0%
! ACTATGAAAGTGTAAAACTG+TGG - chr8_1:17998871-17998890 Msa1182830:CDS 30.0%
!! AATTAGTCTTTGACAGATCA+GGG - chr8_1:17998911-17998930 Msa1182830:CDS 30.0%
TTGAATGTAATAACATGTGC+CGG + chr8_1:17999024-17999043 None:intergenic 30.0%
! AGAGCAACTCTTAATTTTGA+CGG - chr8_1:17999194-17999213 Msa1182830:intron 30.0%
!!! CAACTAGTCTGTTATTTTCT+CGG - chr8_1:17999315-17999334 Msa1182830:intron 30.0%
TCACATTACTAGAGTAACAA+AGG + chr8_1:17999866-17999885 None:intergenic 30.0%
TTGTTACTCTAGTAATGTGA+TGG - chr8_1:17999866-17999885 Msa1182830:intron 30.0%
TACTAAATCAACAAACAAGG+TGG + chr8_1:18000279-18000298 None:intergenic 30.0%
TTGATTCCACATTCTATACA+TGG - chr8_1:18000453-18000472 Msa1182830:intron 30.0%
AAAAGATTAGTGTCTTTGAG+TGG + chr8_1:18000643-18000662 None:intergenic 30.0%
CTCAATGTTAAAGTTTCTCA+CGG - chr8_1:18000823-18000842 Msa1182830:CDS 30.0%
AAGGAAGAAATCTTTCTTCA+AGG - chr8_1:18000956-18000975 Msa1182830:CDS 30.0%
AAATCTTTCTTCAAGGCTTA+AGG - chr8_1:18000963-18000982 Msa1182830:CDS 30.0%
!!! GAACTTGTTTTACAAAGTGA+TGG - chr8_1:18001012-18001031 Msa1182830:CDS 30.0%
!! TGCTCTAAGATAAAATTGTG+TGG + chr8_1:18001229-18001248 None:intergenic 30.0%
! AGAGCAACTCTTAATTTTGA+TGG - chr8_1:17999194-17999213 Msa1182830:intron 30.0%
ATATAATAGCTTCTGCACTT+TGG - chr8_1:18001386-18001405 Msa1182830:intron 30.0%
TATAATAGCTTCTGCACTTT+GGG - chr8_1:18001387-18001406 Msa1182830:intron 30.0%
! TCAATCAGTGAGTAATGTTT+TGG + chr8_1:18001441-18001460 None:intergenic 30.0%
ATTGATCCTAATGATCCATA+TGG - chr8_1:18001456-18001475 Msa1182830:intron 30.0%
!!! AGATTTTGATTGACACTGAT+TGG - chr8_1:18001556-18001575 Msa1182830:intron 30.0%
TATCAGATTATGTGCTTCAA+AGG - chr8_1:18001583-18001602 Msa1182830:intron 30.0%
!!! TTTCTGGTTTGTCCCTTTTA+CGG - chr8_1:17997896-17997915 Msa1182830:CDS 35.0%
CAAATAACCAACACACAAGT+TGG + chr8_1:17998176-17998195 None:intergenic 35.0%
!! CAACTTGTGTGTTGGTTATT+TGG - chr8_1:17998174-17998193 Msa1182830:CDS 35.0%
TTTGTATCCTTGTGTAGACT+TGG + chr8_1:17998215-17998234 None:intergenic 35.0%
ATGAATCATATGGACCGAAT+TGG - chr8_1:17998290-17998309 Msa1182830:CDS 35.0%
!! ACCAAAAGTACTATAGCCAT+CGG - chr8_1:17998397-17998416 Msa1182830:CDS 35.0%
AGATGAAAACAACCGTGAAA+AGG + chr8_1:17998449-17998468 None:intergenic 35.0%
ATGGTGAACTTAACAGTCTT+TGG - chr8_1:17998679-17998698 Msa1182830:CDS 35.0%
TGGTGAACTTAACAGTCTTT+GGG - chr8_1:17998680-17998699 Msa1182830:CDS 35.0%
GAGATCCTTCTACAAAATGA+TGG - chr8_1:17998789-17998808 Msa1182830:CDS 35.0%
!! CAATTAGTCTTTGACAGATC+AGG - chr8_1:17998910-17998929 Msa1182830:CDS 35.0%
TATTATCTGTGTCAACGTGT+CGG - chr8_1:17999063-17999082 Msa1182830:intron 35.0%
!! GAGCAACTCTTAATTTTGAC+GGG - chr8_1:17999195-17999214 Msa1182830:intron 35.0%
!! AGCAACTCTTAATTTTGACG+GGG - chr8_1:17999196-17999215 Msa1182830:intron 35.0%
TGCAAGTTGTTCCAAATTCA+TGG - chr8_1:17999344-17999363 Msa1182830:intron 35.0%
! ACTTTTGTCGTCCATGAATT+TGG + chr8_1:17999358-17999377 None:intergenic 35.0%
CATGGACGACAAAAGTAAAT+AGG - chr8_1:17999362-17999381 Msa1182830:intron 35.0%
TACTCCATCTGAATTTCCTT+CGG + chr8_1:17999394-17999413 None:intergenic 35.0%
AATGAACATGACAGAAGAAG+AGG - chr8_1:17999443-17999462 Msa1182830:intron 35.0%
!!! TGAGGATGGTTTTAGGTATA+CGG - chr8_1:17999466-17999485 Msa1182830:intron 35.0%
! TGGCGATTCTAGTGATTTTA+AGG + chr8_1:17999640-17999659 None:intergenic 35.0%
AACATTGAGATGATTCGATG+TGG + chr8_1:17999660-17999679 None:intergenic 35.0%
CGCTTCATACATAGGTAATT+AGG - chr8_1:17999729-17999748 Msa1182830:intron 35.0%
CATACATAGGTAATTAGGCA+TGG - chr8_1:17999734-17999753 Msa1182830:intron 35.0%
!! TAGTTTTACAGGGTTCAAGT+TGG + chr8_1:17999895-17999914 None:intergenic 35.0%
ATAGACAGGAACTTTAGATG+TGG - chr8_1:17999946-17999965 Msa1182830:intron 35.0%
TACTTACTCATACTGAATCC+AGG + chr8_1:17999975-17999994 None:intergenic 35.0%
CAAACAAGGTGGCTTAAATT+TGG + chr8_1:18000268-18000287 None:intergenic 35.0%
!! GCTCAGTGCTTTAATTTTGA+TGG - chr8_1:18000387-18000406 Msa1182830:intron 35.0%
AAGAAGCCATGTATAGAATG+TGG + chr8_1:18000462-18000481 None:intergenic 35.0%
ATTGCAAAATCAGTGAGTAG+AGG + chr8_1:18000501-18000520 None:intergenic 35.0%
AAAAGAATCACCAATGGCTA+TGG + chr8_1:18000554-18000573 None:intergenic 35.0%
TGCAGTAAAAGAATCACCAA+TGG + chr8_1:18000560-18000579 None:intergenic 35.0%
TTTGAGTGGAAGAAAACCAA+AGG + chr8_1:18000629-18000648 None:intergenic 35.0%
! TTTCTGAGAAAACTGTTGTG+TGG - chr8_1:18000689-18000708 Msa1182830:intron 35.0%
!! AGTTCCACTTTTGATCCTTT+TGG + chr8_1:18000739-18000758 None:intergenic 35.0%
ATGGTAAATTCAACAGTCTG+TGG - chr8_1:18000881-18000900 Msa1182830:CDS 35.0%
TGGTAAATTCAACAGTCTGT+GGG - chr8_1:18000882-18000901 Msa1182830:CDS 35.0%
AAGGAGACAGATTTCAGTAA+AGG - chr8_1:18000982-18001001 Msa1182830:CDS 35.0%
AGACAGATTTCAGTAAAGGA+AGG - chr8_1:18000986-18001005 Msa1182830:CDS 35.0%
!! AATTGGTCTTTGACAAGTCA+GGG - chr8_1:18001136-18001155 Msa1182830:intron 35.0%
!! TCAGGGTACTTGTATGTTTT+AGG - chr8_1:18001153-18001172 Msa1182830:intron 35.0%
TCACTCTTAGTTGAACTCTT+AGG + chr8_1:18001291-18001310 None:intergenic 35.0%
AAGAGTTCAACTAAGAGTGA+AGG - chr8_1:18001291-18001310 Msa1182830:intron 35.0%
TGTACTTATACTGTTAGTGC+AGG - chr8_1:18001348-18001367 Msa1182830:intron 35.0%
!! TGTTTTGGACACTGACATTT+TGG + chr8_1:18001426-18001445 None:intergenic 35.0%
TGAAGCACATAATCTGATAG+AGG + chr8_1:18001582-18001601 None:intergenic 35.0%
!! AAGAAGTTGCCACTTTCAAA+TGG - chr8_1:18001696-18001715 Msa1182830:intron 35.0%
!! AGAAGTTGCCACTTTCAAAT+GGG - chr8_1:18001697-18001716 Msa1182830:intron 35.0%
!! AGAGTTGATGCAACTCTTAA+TGG - chr8_1:18001720-18001739 Msa1182830:CDS 35.0%
!! ACACACAAGTTGGTATGCTT+TGG + chr8_1:17998166-17998185 None:intergenic 40.0%
AAGCATACCAACTTGTGTGT+TGG - chr8_1:17998166-17998185 Msa1182830:CDS 40.0%
AATCAGTGAGTAGAGGAGAA+GGG + chr8_1:17998350-17998369 None:intergenic 40.0%
AAATCAGTGAGTAGAGGAGA+AGG + chr8_1:17998351-17998370 None:intergenic 40.0%
! ACCGATGGCTATAGTACTTT+TGG + chr8_1:17998401-17998420 None:intergenic 40.0%
TGCAGTAAAAGAATCACCGA+TGG + chr8_1:17998416-17998435 None:intergenic 40.0%
! CACAAACCAACTCCTTTTCA+CGG - chr8_1:17998434-17998453 Msa1182830:CDS 40.0%
AAACAACCGTGAAAAGGAGT+TGG + chr8_1:17998443-17998462 None:intergenic 40.0%
AGAAACTCACTGCCAATGAT+GGG - chr8_1:17998550-17998569 Msa1182830:CDS 40.0%
TTCCATAGCATGTCACCATT+TGG + chr8_1:17998591-17998610 None:intergenic 40.0%
! CTTGGTGATAGAGTTTCTTG+TGG - chr8_1:17998621-17998640 Msa1182830:CDS 40.0%
TGTGGTGTGTTCAATGACAT+TGG - chr8_1:17998639-17998658 Msa1182830:CDS 40.0%
TAAAACTGTGGATCCAGTTC+TGG - chr8_1:17998883-17998902 Msa1182830:CDS 40.0%
GACTAATTGAGTTCCAGAAC+TGG + chr8_1:17998899-17998918 None:intergenic 40.0%
! ATCAGGGTACTTGTATGTCT+TGG - chr8_1:17998927-17998946 Msa1182830:CDS 40.0%
! TCAGGGTACTTGTATGTCTT+GGG - chr8_1:17998928-17998947 Msa1182830:CDS 40.0%
ATGCCTTAGATCATAGGTGT+CGG + chr8_1:17998964-17998983 None:intergenic 40.0%
TCACTCTCAGTTGAACTCTT+TGG + chr8_1:17999242-17999261 None:intergenic 40.0%
AAGAGTTCAACTGAGAGTGA+AGG - chr8_1:17999242-17999261 Msa1182830:intron 40.0%
GGACGACAAAAGTAAATAGG+TGG - chr8_1:17999365-17999384 Msa1182830:intron 40.0%
AGTAAATAGGTGGAAACCGA+AGG - chr8_1:17999375-17999394 Msa1182830:intron 40.0%
GAAACCGAAGGAAATTCAGA+TGG - chr8_1:17999387-17999406 Msa1182830:intron 40.0%
TGGCATCATTGGTACGAAAA+TGG - chr8_1:17999521-17999540 Msa1182830:intron 40.0%
ATCATTGGTACGAAAATGGC+CGG - chr8_1:17999525-17999544 Msa1182830:intron 40.0%
ATTACCTATGTATGAAGCGC+AGG + chr8_1:17999728-17999747 None:intergenic 40.0%
GAACTTTAGATGTGGTTACC+TGG - chr8_1:17999954-17999973 Msa1182830:intron 40.0%
! CTTGTGTAAAGTGGACTTTC+AGG + chr8_1:18000169-18000188 None:intergenic 40.0%
GGACCAAATTGGAAGACTAA+TGG - chr8_1:18000365-18000384 Msa1182830:intron 40.0%
!! GAGCCATTAGTCTTCCAATT+TGG + chr8_1:18000371-18000390 None:intergenic 40.0%
ATCAGTGAGTAGAGGAGAAA+GGG + chr8_1:18000493-18000512 None:intergenic 40.0%
AATCAGTGAGTAGAGGAGAA+AGG + chr8_1:17998350-17998369 None:intergenic 40.0%
!! ACCAAAAGTACCATAGCCAT+TGG - chr8_1:18000541-18000560 Msa1182830:intron 40.0%
! ACCAATGGCTATGGTACTTT+TGG + chr8_1:18000545-18000564 None:intergenic 40.0%
ACTGTTGTGTGGTATGCTAA+TGG - chr8_1:18000700-18000719 Msa1182830:intron 40.0%
!! ACTTTTGATCCTTTTGGTGC+AGG + chr8_1:18000733-18000752 None:intergenic 40.0%
TGCACCAAAAGGATCAAAAG+TGG - chr8_1:18000732-18000751 Msa1182830:intron 40.0%
TGGAACTCACTGCTAATGAT+GGG - chr8_1:18000752-18000771 Msa1182830:intron 40.0%
!! TTGGTGCTAACTTCTCCAAA+TGG - chr8_1:18000775-18000794 Msa1182830:intron 40.0%
TAACTTCTCCAAATGGTGTC+AGG - chr8_1:18000782-18000801 Msa1182830:intron 40.0%
TTCCATAACCTGACACCATT+TGG + chr8_1:18000793-18000812 None:intergenic 40.0%
GTCAGGTTATGGAATACTGA+AGG - chr8_1:18000799-18000818 Msa1182830:CDS 40.0%
CACAACTTGAGAAGGCAATA+AGG + chr8_1:18000932-18000951 None:intergenic 40.0%
! CTTATTGCCTTCTCAAGTTG+TGG - chr8_1:18000930-18000949 Msa1182830:CDS 40.0%
CCTTTATCCACAACTTGAGA+AGG + chr8_1:18000940-18000959 None:intergenic 40.0%
! CCTTCTCAAGTTGTGGATAA+AGG - chr8_1:18000937-18000956 Msa1182830:CDS 40.0%
AAATCCAGTACATCAAGTGC+TGG - chr8_1:18001108-18001127 Msa1182830:intron 40.0%
!! CAATTGGTCTTTGACAAGTC+AGG - chr8_1:18001135-18001154 Msa1182830:intron 40.0%
GTTCAACTAAGAGTGAAGGT+TGG - chr8_1:18001295-18001314 Msa1182830:intron 40.0%
TATACTGTTAGTGCAGGTAG+TGG - chr8_1:18001354-18001373 Msa1182830:intron 40.0%
CAGCTTCCATATGGATCATT+AGG + chr8_1:18001465-18001484 None:intergenic 40.0%
CTACACTGCTCTTCAGTAAA+AGG + chr8_1:18001609-18001628 None:intergenic 40.0%
! CTTTTACTGAAGAGCAGTGT+AGG - chr8_1:18001607-18001626 Msa1182830:intron 40.0%
GCAGTGTAGGAAATCTTGTA+TGG - chr8_1:18001620-18001639 Msa1182830:intron 40.0%
GGATGATTGTTTGTGTTCTG+TGG - chr8_1:18001641-18001660 Msa1182830:intron 40.0%
!! TGTGTTCTGTGGCTATTTTC+AGG - chr8_1:18001652-18001671 Msa1182830:intron 40.0%
!!! TCTGTGGCTATTTTCAGGTT+AGG - chr8_1:18001657-18001676 Msa1182830:intron 40.0%
TCAGGTTAGGTGATAGTTGT+TGG - chr8_1:18001670-18001689 Msa1182830:intron 40.0%
TCAACTCTCCCATTTGAAAG+TGG + chr8_1:18001708-18001727 None:intergenic 40.0%
! TGCAACTCTTAATGGTGCTA+AGG - chr8_1:18001728-18001747 Msa1182830:CDS 40.0%
! CTAATTTGTGACAGGTATGG+TGG + chr8_1:18001793-18001812 None:intergenic 40.0%
ACAAACCAGAAACCAATGCG+CGG + chr8_1:17997888-17997907 None:intergenic 45.0%
GAGTTGACCAAGTCTACACA+AGG - chr8_1:17998205-17998224 Msa1182830:CDS 45.0%
ATCAGTGAGTAGAGGAGAAG+GGG + chr8_1:17998349-17998368 None:intergenic 45.0%
GGTTTGAGCTACAACACTGA+TGG + chr8_1:17998380-17998399 None:intergenic 45.0%
GAGAAACTCACTGCCAATGA+TGG - chr8_1:17998549-17998568 Msa1182830:CDS 45.0%
CTCCAAATGGTGACATGCTA+TGG - chr8_1:17998586-17998605 Msa1182830:CDS 45.0%
CATTGGAAACTCTGTGCTTG+AGG - chr8_1:17998656-17998675 Msa1182830:CDS 45.0%
AACAACCTGAGAAGGCAAGA+AGG + chr8_1:17998730-17998749 None:intergenic 45.0%
! TGCCTTCTCAGGTTGTTGAA+AGG - chr8_1:17998733-17998752 Msa1182830:CDS 45.0%
AGCCTTTCAACAACCTGAGA+AGG + chr8_1:17998738-17998757 None:intergenic 45.0%
! CCTATGATCTAAGGCATGTC+CGG - chr8_1:17998967-17998986 Msa1182830:CDS 45.0%
!! ATGAAGCACCGACATAGACA+TGG + chr8_1:17999097-17999116 None:intergenic 45.0%
! TGTCTATGTCGGTGCTTCAT+AGG - chr8_1:17999097-17999116 Msa1182830:intron 45.0%
GTTCAACTGAGAGTGAAGGT+TGG - chr8_1:17999246-17999265 Msa1182830:intron 45.0%
ACATGACAGAAGAAGAGGTG+AGG - chr8_1:17999448-17999467 Msa1182830:intron 45.0%
! GAAGAGGTGAGGATGGTTTT+AGG - chr8_1:17999459-17999478 Msa1182830:intron 45.0%
!!! GGATGGTTTTAGGTATACGG+TGG - chr8_1:17999469-17999488 Msa1182830:intron 45.0%
GCAAAGAAGGTTGGCATCAT+TGG - chr8_1:17999510-17999529 Msa1182830:intron 45.0%
CTATGTATGAAGCGCAGGAA+AGG + chr8_1:17999723-17999742 None:intergenic 45.0%
CTTTCCTGCGCTTCATACAT+AGG - chr8_1:17999721-17999740 Msa1182830:intron 45.0%
GTCCACTTTACACAAGTCCA+TGG - chr8_1:18000173-18000192 Msa1182830:intron 45.0%
GACCATGGACTTGTGTAAAG+TGG + chr8_1:18000178-18000197 None:intergenic 45.0%
GGTTTGAGCTACAACACAGA+TGG + chr8_1:18000524-18000543 None:intergenic 45.0%
GTGGAACTCACTGCTAATGA+TGG - chr8_1:18000751-18000770 Msa1182830:intron 45.0%
!! ACTCACTGCTAATGATGGGT+TGG - chr8_1:18000756-18000775 Msa1182830:intron 45.0%
! CTCCAAATGGTGTCAGGTTA+TGG - chr8_1:18000788-18000807 Msa1182830:intron 45.0%
!! GAAGGCAATAAGGTGTCACT+AGG + chr8_1:18000922-18000941 None:intergenic 45.0%
!! ATCAAGTGCTGGAGCTCAAT+TGG - chr8_1:18001119-18001138 Msa1182830:intron 45.0%
AAAGGGCTTAGACCATACTG+TGG + chr8_1:18001322-18001341 None:intergenic 45.0%
TGCAAGCCAGATTTCATCCA+AGG - chr8_1:18001483-18001502 Msa1182830:intron 45.0%
GCACATCCTTGGATGAAATC+TGG + chr8_1:18001492-18001511 None:intergenic 45.0%
GATCATCTTCTGCACATCCT+TGG + chr8_1:18001503-18001522 None:intergenic 45.0%
! GACAGGTATGGTGGAACTAT+GGG + chr8_1:18001784-18001803 None:intergenic 45.0%
! TGACAGGTATGGTGGAACTA+TGG + chr8_1:18001785-18001804 None:intergenic 45.0%
!! ATATTATTATAAATTATAGT+AGG + chr8_1:18000097-18000116 None:intergenic 5.0%
GTTGGAACGACGACATCCAA+AGG - chr8_1:17997827-17997846 Msa1182830:CDS 50.0%
! CTGGTGCTAGCTTCTCCAAA+TGG - chr8_1:17998573-17998592 Msa1182830:CDS 50.0%
TGACATGCTATGGAACCCTG+AGG - chr8_1:17998596-17998615 Msa1182830:CDS 50.0%
GACATGCTATGGAACCCTGA+GGG - chr8_1:17998597-17998616 Msa1182830:CDS 50.0%
CTCTATCACCAAGTCCCTCA+GGG + chr8_1:17998614-17998633 None:intergenic 50.0%
ACTCTATCACCAAGTCCCTC+AGG + chr8_1:17998615-17998634 None:intergenic 50.0%
GGAAACTCTGTGCTTGAGGA+TGG - chr8_1:17998660-17998679 Msa1182830:CDS 50.0%
! GCACCGACACCTATGATCTA+AGG - chr8_1:17998958-17998977 Msa1182830:CDS 50.0%
CCGGACATGCCTTAGATCAT+AGG + chr8_1:17998970-17998989 None:intergenic 50.0%
TGCGGTGTCCATGTCTATGT+CGG - chr8_1:17999086-17999105 Msa1182830:intron 50.0%
! TGTCGGTGCTTCATAGGTCT+TGG - chr8_1:17999103-17999122 Msa1182830:intron 50.0%
! GTCGGTGCTTCATAGGTCTT+GGG - chr8_1:17999104-17999123 Msa1182830:intron 50.0%
GACAGAAGAAGAGGTGAGGA+TGG - chr8_1:17999452-17999471 Msa1182830:intron 50.0%
!!! GGTTTTAGGTATACGGTGGC+CGG - chr8_1:17999473-17999492 Msa1182830:intron 50.0%
ATTGGTACGAAAATGGCCGG+AGG - chr8_1:17999528-17999547 Msa1182830:intron 50.0%
AAGCAACCATGGAGATGTGC+TGG + chr8_1:18000587-18000606 None:intergenic 50.0%
CCAGAAGGTGAAAGCAACCA+TGG + chr8_1:18000598-18000617 None:intergenic 50.0%
! CCATGGTTGCTTTCACCTTC+TGG - chr8_1:18000595-18000614 Msa1182830:intron 50.0%
CCAAAGGCAAAGTCACCAGA+AGG + chr8_1:18000613-18000632 None:intergenic 50.0%
CCTTCTGGTGACTTTGCCTT+TGG - chr8_1:18000610-18000629 Msa1182830:intron 50.0%
CACGGTGTGTTGAATGACAC+TGG - chr8_1:18000841-18000860 Msa1182830:CDS 50.0%
CACTGGCAACTTTGTGCTTC+AGG - chr8_1:18000858-18000877 Msa1182830:CDS 50.0%
GGCAACTTTGTGCTTCAGGA+TGG - chr8_1:18000862-18000881 Msa1182830:CDS 50.0%
AGCTCCAGCACTTGATGTAC+TGG + chr8_1:18001115-18001134 None:intergenic 50.0%
GTGAAGGTTGGACCACAGTA+TGG - chr8_1:18001307-18001326 Msa1182830:intron 50.0%
! AGTGCAGGTAGTGGTGTTTG+TGG - chr8_1:18001363-18001382 Msa1182830:intron 50.0%
! TCTGGCTTGCAGCTTCCATA+TGG + chr8_1:18001474-18001493 None:intergenic 50.0%
! AGGTATGGTGGAACTATGGG+AGG + chr8_1:18001781-18001800 None:intergenic 50.0%
ACATCCAAAGGCAGCAGGAG+TGG - chr8_1:17997839-17997858 Msa1182830:CDS 55.0%
CAAGATGACTCACCGCGCAT+TGG - chr8_1:17997873-17997892 Msa1182830:CDS 55.0%
!! ACTCACCGCGCATTGGTTTC+TGG - chr8_1:17997880-17997899 Msa1182830:CDS 55.0%
GAGGAGAAGGGGAAGTAGAG+AGG + chr8_1:17998338-17998357 None:intergenic 55.0%
!! ACTCACTGCCAATGATGGGC+TGG - chr8_1:17998554-17998573 Msa1182830:CDS 55.0%
AGCTAGCACCAGCCCATCAT+TGG + chr8_1:17998565-17998584 None:intergenic 55.0%
CTATGGAACCCTGAGGGACT+TGG - chr8_1:17998603-17998622 Msa1182830:CDS 55.0%
TGACGCCTTCTTGCCTTCTC+AGG - chr8_1:17998722-17998741 Msa1182830:CDS 55.0%
!! AACATGTGCCGGTGTCGTGT+CGG + chr8_1:17999013-17999032 None:intergenic 55.0%
! TCTGTGTCAACGTGTCGGTG+CGG - chr8_1:17999068-17999087 Msa1182830:intron 55.0%
AGAGACTCACCCTGTCGTCT+TGG + chr8_1:17999568-17999587 None:intergenic 55.0%
AAGACGACAGGGTGAGTCTC+TGG - chr8_1:17999567-17999586 Msa1182830:intron 55.0%
CACAAGCCAGCACATCTCCA+TGG - chr8_1:18000578-18000597 Msa1182830:intron 55.0%
GGAGACACTCCTGCACCAAA+AGG - chr8_1:18000721-18000740 Msa1182830:intron 55.0%
CGACGACATCCAAAGGCAGC+AGG - chr8_1:17997834-17997853 Msa1182830:CDS 60.0%
CAGCAGGAGTGGCAGCAAGA+AGG - chr8_1:17997850-17997869 Msa1182830:CDS 60.0%
GACTGACACGTGTCGGACAC+CGG + chr8_1:17998989-17999008 None:intergenic 60.0%
TGTCGGGGACTGACACGTGT+CGG + chr8_1:17998996-17999015 None:intergenic 60.0%
!! ACATGTGCCGGTGTCGTGTC+GGG + chr8_1:17999012-17999031 None:intergenic 60.0%
ACCTTCTTTGCCACACCGCC+CGG + chr8_1:17999501-17999520 None:intergenic 60.0%
GGCGGTGTGGCAAAGAAGGT+TGG - chr8_1:17999501-17999520 Msa1182830:intron 60.0%
TGGAGTGGCTCCAAGACGAC+AGG - chr8_1:17999555-17999574 Msa1182830:intron 60.0%
GGAGTGGCTCCAAGACGACA+GGG - chr8_1:17999556-17999575 Msa1182830:intron 60.0%
ATGGCCAGTGGCGTAGGGGA+TGG - chr8_1:17997789-17997808 Msa1182830:CDS 65.0%
CCAGTGGCGTAGGGGATGGA+CGG - chr8_1:17997793-17997812 Msa1182830:CDS 65.0%
CGTAGGGGATGGACGGCTGT+TGG - chr8_1:17997800-17997819 Msa1182830:CDS 65.0%
TGGACGGCTGTTGGCAGCGT+TGG - chr8_1:17997809-17997828 Msa1182830:CDS 65.0%
GCTGCCACTCCTGCTGCCTT+TGG + chr8_1:17997846-17997865 None:intergenic 65.0%
GTCAGTCCCCGACACGACAC+CGG - chr8_1:17999002-17999021 Msa1182830:CDS 65.0%
!! CATGTGCCGGTGTCGTGTCG+GGG + chr8_1:17999011-17999030 None:intergenic 65.0%
CGAAAATGGCCGGAGGCAGC+TGG - chr8_1:17999535-17999554 Msa1182830:intron 65.0%
CCGTCCATCCCCTACGCCAC+TGG + chr8_1:17997796-17997815 None:intergenic 70.0%
AGGTATACGGTGGCCGGCGC+CGG - chr8_1:17999479-17999498 Msa1182830:intron 70.0%
GCCGGGCGGTGTGGCAAAGA+AGG - chr8_1:17999497-17999516 Msa1182830:intron 70.0%
ATGGCCGGAGGCAGCTGGAG+TGG - chr8_1:17999540-17999559 Msa1182830:intron 70.0%
GGAGCCACTCCAGCTGCCTC+CGG + chr8_1:17999547-17999566 None:intergenic 70.0%
GGTATACGGTGGCCGGCGCC+GGG - chr8_1:17999480-17999499 Msa1182830:intron 75.0%
TTTGCCACACCGCCCGGCGC+CGG + chr8_1:17999495-17999514 None:intergenic 75.0%
!! ATACGGTGGCCGGCGCCGGG+CGG - chr8_1:17999483-17999502 Msa1182830:intron 80.0%
!! GTGGCCGGCGCCGGGCGGTG+TGG - chr8_1:17999488-17999507 Msa1182830:intron 90.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 17997789 18001812 17997789 ID=Msa1182830;Name=Msa1182830
chr8_1 mRNA 17997789 18001812 17997789 ID=Msa1182830-mRNA-1;Parent=Msa1182830;Name=Msa1182830-mRNA-1;_AED=0.21;_eAED=0.22;_QI=0|0|0|0.66|1|1|3|0|570
chr8_1 exon 18001700 18001812 18001700 ID=Msa1182830-mRNA-1:exon:6913;Parent=Msa1182830-mRNA-1
chr8_1 exon 18000791 18001113 18000791 ID=Msa1182830-mRNA-1:exon:6912;Parent=Msa1182830-mRNA-1
chr8_1 exon 17997789 17999065 17997789 ID=Msa1182830-mRNA-1:exon:6911;Parent=Msa1182830-mRNA-1
chr8_1 CDS 18001700 18001812 18001700 ID=Msa1182830-mRNA-1:cds;Parent=Msa1182830-mRNA-1
chr8_1 CDS 18000791 18001113 18000791 ID=Msa1182830-mRNA-1:cds;Parent=Msa1182830-mRNA-1
chr8_1 CDS 17997789 17999065 17997789 ID=Msa1182830-mRNA-1:cds;Parent=Msa1182830-mRNA-1
Gene Sequence

>Msa1182830

ATGGCCAGTGGCGTAGGGGATGGACGGCTGTTGGCAGCGTTGGAACGACGACATCCAAAGGCAGCAGGAGTGGCAGCAAGAAGGCAAGATGACTCACCGCGCATTGGTTTCTGGTTTTCTTCTACTAGAAGATACCATGTTTTTTTGCTTTCCATTTGGTATGCAAACATTTCTGAGAAACTCACTGCCAATGATGGGCTGGTGCTAGCTTCTCCAAATGGTGACATGCTATGGAACCCTGAGGGACTTGGTGATAGAGTTTCTTGTGGTGTGTTCAATGACATTGGAAACTCTGTGCTTGAGGATGGTGAACTTAACAGTCTTTGGGAGACTTTCAAGTTTTCTAGTGACGCCTTCTTGCCTTCTCAGGTTGTTGAAAGGCTTAAAGAGACTAATTTCAGTAAAGGAAGGTGTGAGATCCTTCTACAAAATGATGCTCAAACCAAAAGTACCATAGCCATTGGTGATTCTTTTACTGCACAAGCCAGCACATCTCCATGGTTGCTTTCACCTTCTGGTGACTTTGCCTTTGGTTTTCTTCCACTCAAAGACACTAATCTTTTTTTGCTTTCAATTTGGTATCCCAAAATTTCTGAGAAAACTGTTGTGTGGTATGCTAATGGAGACACTCCTGCACCAAAAGGATCAAAAGTGGAACTCACTGCTAATGATGGGTTGGTGCTAACTTCTCCAAATGGTGTCAGGTTATGGAATACTGAAGGACTCAATGTTAAAGTTTCTCACGGTGTGTTGAATGACACTGGCAACTTTGTGCTTCAGGATGGTAAATTCAACAGTCTGTGGGAGACTTTCAAGTTTCCTAGTGACACCTTATTGCCTTCTCAAGTTGTGGATAAAGGAAGAAATCTTTCTTCAAGGCTTAAGGAGACAGATTTCAGTAAAGGAAGGTTTGAACTTGTTTTACAAAGTGATGGTAATCTTGTTATGCATTCTATTAATTTACCATCTGGTTATGTTAATGAAAACTACTTTGAAAGTAATACTGTTAAATCCAGTACATCAAGTGCTGGAGCTCAATTGGTCTTTGACAAGTCAGGGTACTTGTATGTTTTAGGAGAGAACAATGAGAAATATAATGTGTTTGAAGAAGAGAGTAACGTCTCGACCACACAATTTTATCTTAGAGCAACTCTTAATTTTGATGGAGTGTTCACACTTTACAAGCATCCTAAGAGTTCAACTAAGAGTGAAGGTTGGACCACAGTATGGTCTAAGCCCTTTAATATATGTACTTATACTGTTAGTGCAGGTAGTGGTGTTTGTGGATATAATAGCTTCTGCACTTTGGGAGTTGATAAGAGACCAAAATGTCAGTGTCCAAAACATTACTCACTGATTGATCCTAATGATCCATATGGAAGCTGCAAGCCAGATTTCATCCAAGGATGTGCAGAAGATGATCCAAGTAAAAAGAGAAATGATCTCTATGAGTTTGAGATTTTGATTGACACTGATTGGCCTCTATCAGATTATGTGCTTCAAAGGCCTTTTACTGAAGAGCAGTGTAGGAAATCTTGTATGGATGATTGTTTGTGTTCTGTGGCTATTTTCAGGTTAGGTGATAGTTGTTGGAAGAAGAAGTTGCCACTTTCAAATGGGAGAGTTGATGCAACTCTTAATGGTGCTAAGGCTTTCTTGAAAGTGAGAAAAGATAATGCCTCCCATAGTTCCACCATACCTGTCACAAATTAG
Protein sequence

>Msa1182830

MASGVGDGRLLAALERRHPKAAGVAARRQDDSPRIGFWFSSTRRYHVFLLSIWYANISEKLTANDGLVLASPNGDMLWNPEGLGDRVSCGVFNDIGNSVLEDGELNSLWETFKFSSDAFLPSQVVERLKETNFSKGRCEILLQNDAQTKSTIAIGDSFTAQASTSPWLLSPSGDFAFGFLPLKDTNLFLLSIWYPKISEKTVVWYANGDTPAPKGSKVELTANDGLVLTSPNGVRLWNTEGLNVKVSHGVLNDTGNFVLQDGKFNSLWETFKFPSDTLLPSQVVDKGRNLSSRLKETDFSKGRFELVLQSDGNLVMHSINLPSGYVNENYFESNTVKSSTSSAGAQLVFDKSGYLYVLGENNEKYNVFEEESNVSTTQFYLRATLNFDGVFTLYKHPKSSTKSEGWTTVWSKPFNICTYTVSAGSGVCGYNSFCTLGVDKRPKCQCPKHYSLIDPNDPYGSCKPDFIQGCAEDDPSKKRNDLYEFEILIDTDWPLSDYVLQRPFTEEQCRKSCMDDCLCSVAIFRLGDSCWKKKLPLSNGRVDATLNGAKAFLKVRKDNASHSSTIPVTN*