AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1184610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1184610 MtrunA17_Chr8g0349961 92.334 287 22 0 8 294 1 287 0.0 530
Msa1184610 MtrunA17_Chr8g0353241 91.398 279 24 0 8 286 1 279 0.0 510
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1184610 AT5G61760.1 52.669 281 122 4 11 280 2 282 7.79e-107 313
Msa1184610 AT5G61760.2 52.669 281 122 4 11 280 2 282 7.79e-107 313
Msa1184610 AT5G07370.2 50.000 280 129 5 9 280 1 277 2.01e-97 289
Msa1184610 AT5G07370.3 50.000 280 129 5 9 280 1 277 2.01e-97 289
Msa1184610 AT5G07370.1 50.000 280 129 5 9 280 1 277 2.01e-97 289
Msa1184610 AT5G07370.4 50.000 280 129 5 9 280 1 277 2.01e-97 289

Find 78 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTTCCACAATAAAATT+AGG 0.168084 8_1:+20533290 Msa1184610:CDS
AGGGAACAATTTGAGAATAT+TGG 0.214068 8_1:-20533084 None:intergenic
AGGCTGCCATAATTTGTTAT+TGG 0.215550 8_1:-20533345 None:intergenic
ATGATGCTTTAATTTCAATT+TGG 0.241118 8_1:+20534058 Msa1184610:three_prime_UTR
GCTATTGATCATAATTTCTT+AGG 0.268767 8_1:+20533665 Msa1184610:CDS
GGCTGCCATAATTTGTTATT+GGG 0.280852 8_1:-20533344 None:intergenic
GACAGCGGGGTTAGTGTAAT+TGG 0.293661 8_1:-20533186 None:intergenic
TCTACACCCTCACCTTGTTT+TGG 0.306627 8_1:+20533151 Msa1184610:CDS
AATCTTCCACAATAAAATTA+GGG 0.316033 8_1:+20533291 Msa1184610:CDS
GCCTGATAGAAAGTTTCTTA+TGG 0.324838 8_1:+20533364 Msa1184610:CDS
TTCTTTGATTAAGTTTGTTA+AGG 0.365539 8_1:+20533697 Msa1184610:CDS
TCCATAAGAAACTTTCTATC+AGG 0.370078 8_1:-20533365 None:intergenic
TAAGTTTGTTAAGGATGTTT+TGG 0.378614 8_1:+20533706 Msa1184610:CDS
TGACCAGCAACTTGGTGGTC+TGG 0.381341 8_1:-20532927 None:intergenic
GATTACTAGAAAGTATGTTT+TGG 0.391004 8_1:+20534080 Msa1184610:three_prime_UTR
GGTCCACTGATAGACGATTC+TGG 0.393481 8_1:+20532972 Msa1184610:CDS
GTTGACGCCTCCGACGGTTC+TGG 0.394613 8_1:+20533128 Msa1184610:CDS
AATAAAATGACTACTTTGTA+AGG 0.397351 8_1:+20534007 Msa1184610:three_prime_UTR
TCCAACAATTCCTCCAAAAC+AGG 0.405671 8_1:-20533488 None:intergenic
ATCCCAGACCACCAAGTTGC+TGG 0.418559 8_1:+20532924 Msa1184610:CDS
AAATTAGGGTTTAGAGTCTC+CGG 0.423865 8_1:+20533305 Msa1184610:CDS
GACCAGCAACTTGGTGGTCT+GGG 0.429133 8_1:-20532926 None:intergenic
ATCTTGATGTCGACGACAGC+GGG 0.442120 8_1:-20533200 None:intergenic
AGGAAATTTCTTTCGTCGAA+TGG 0.444436 8_1:+20533419 Msa1184610:CDS
TTGGCTGGTTTAGGTGATGT+TGG 0.452772 8_1:+20533725 Msa1184610:CDS
GGATGCGTGGATCGGAATAG+AGG 0.453423 8_1:-20533058 None:intergenic
GTTTCAGTGCCGTGAAAAGA+AGG 0.453560 8_1:-20533104 None:intergenic
ATACAGCTTCAGTAGCAAAA+TGG 0.454488 8_1:+20532890 Msa1184610:intron
TGGGTCCCAATAACAAATTA+TGG 0.455132 8_1:+20533339 Msa1184610:CDS
GGTTTAGAGTCTCCGGGTTG+AGG 0.464820 8_1:+20533312 Msa1184610:CDS
AATTGTTGGAGCTTAAGAAA+TGG 0.470611 8_1:+20533501 Msa1184610:CDS
CTTGGTGACCAGCAACTTGG+TGG 0.472242 8_1:-20532932 None:intergenic
CCTCGTTCGTCGTCTTGAAG+AGG 0.475806 8_1:-20533008 None:intergenic
GCTCACGTCGTTGATGCTAA+AGG 0.487055 8_1:+20533641 Msa1184610:CDS
GTGAAAGATTGCACTGATGT+AGG 0.492754 8_1:+20533817 Msa1184610:three_prime_UTR
AGTCTCCGGGTTGAGGTCTG+TGG 0.499059 8_1:+20533319 Msa1184610:CDS
GCATTTCCGAATGTAGTCTT+CGG 0.501435 8_1:-20533252 None:intergenic
AATCTTCCGAAGACTACATT+CGG 0.505169 8_1:+20533246 Msa1184610:CDS
GGAGCTTAAGAAATGGTTTG+AGG 0.507811 8_1:+20533508 Msa1184610:CDS
GTCTCCGGGTTGAGGTCTGT+GGG 0.516653 8_1:+20533320 Msa1184610:CDS
TTGCTTGGTGACCAGCAACT+TGG 0.518603 8_1:-20532935 None:intergenic
GAACAATTTGAGAATATTGG+AGG 0.521764 8_1:-20533081 None:intergenic
ATGTAGTCTTCGGAAGATTG+AGG 0.522222 8_1:-20533242 None:intergenic
TGAGAAAGAATGATTATCAC+TGG 0.524715 8_1:+20533784 Msa1184610:exon
ATCTGCTGATGATGTAATGT+TGG 0.526177 8_1:+20533391 Msa1184610:CDS
TGAAAGATTGCACTGATGTA+GGG 0.526286 8_1:+20533818 Msa1184610:three_prime_UTR
TTATTCGTGTTCTGTTCTTG+TGG 0.531954 8_1:+20533550 Msa1184610:CDS
GCTGTCGTCGACATCAAGAT+TGG 0.540324 8_1:+20533203 Msa1184610:CDS
AACAATTTGAGAATATTGGA+GGG 0.542391 8_1:-20533080 None:intergenic
TGTAGTCTTCGGAAGATTGA+GGG 0.546321 8_1:-20533241 None:intergenic
GGTGTAGAAAGCAAGTTCGT+TGG 0.549867 8_1:-20533033 None:intergenic
TCAAGATTGGTTCTCGAACA+TGG 0.560230 8_1:+20533216 Msa1184610:CDS
GATGCGTGGATCGGAATAGA+GGG 0.565710 8_1:-20533057 None:intergenic
AATTAGGGTTTAGAGTCTCC+GGG 0.566602 8_1:+20533306 Msa1184610:CDS
ATTGGAGGGGATGCGTGGAT+CGG 0.574959 8_1:-20533066 None:intergenic
ACAATTTGAGAATATTGGAG+GGG 0.576270 8_1:-20533079 None:intergenic
ATTGATCATAATTTCTTAGG+CGG 0.581811 8_1:+20533668 Msa1184610:CDS
AGAATATTGGAGGGGATGCG+TGG 0.584053 8_1:-20533071 None:intergenic
AGAACCGTCGGAGGCGTCAA+CGG 0.588605 8_1:-20533126 None:intergenic
TTTCCAGAATCGTCTATCAG+TGG 0.594735 8_1:-20532975 None:intergenic
AAGATTCTCGATCTTTCTGA+AGG 0.595546 8_1:-20533274 None:intergenic
ATATCTTCCAAAACAAGGTG+AGG 0.604027 8_1:-20533158 None:intergenic
AATTCCGCGATGTATATTAG+CGG 0.604888 8_1:+20533868 Msa1184610:three_prime_UTR
GATGGATAGATGAATAGACT+TGG 0.609788 8_1:+20533913 Msa1184610:three_prime_UTR
TTTCAGTGCCGTGAAAAGAA+GGG 0.610037 8_1:-20533103 None:intergenic
TGATGTAGGGAACAAATGAC+AGG 0.615353 8_1:+20533831 Msa1184610:three_prime_UTR
GAAACCGTTGACGCCTCCGA+CGG 0.617951 8_1:+20533122 Msa1184610:CDS
CCTCTTCAAGACGACGAACG+AGG 0.625721 8_1:+20533008 Msa1184610:CDS
AATCTTGATGTCGACGACAG+CGG 0.639445 8_1:-20533201 None:intergenic
ATGCGATATCTTCCAAAACA+AGG 0.648535 8_1:-20533163 None:intergenic
TATCTTCCAAAACAAGGTGA+GGG 0.651339 8_1:-20533157 None:intergenic
AGGGTGTAGACCAGAACCGT+CGG 0.661282 8_1:-20533138 None:intergenic
AATCGTCTATCAGTGGACCT+AGG 0.661906 8_1:-20532968 None:intergenic
GGGACCCACAGACCTCAACC+CGG 0.672977 8_1:-20533324 None:intergenic
TCTTGATGTCGACGACAGCG+GGG 0.688730 8_1:-20533199 None:intergenic
GTGTAGACCAGAACCGTCGG+AGG 0.689628 8_1:-20533135 None:intergenic
GCGTGGATCGGAATAGAGGG+AGG 0.703005 8_1:-20533054 None:intergenic
TCTTCCGCTAATATACATCG+CGG 0.744266 8_1:-20533872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTTCTTTGAAT+CGG - chr8_1:20532233-20532252 None:intergenic 10.0%
!! TTACATATTTACATAGTTTA+GGG - chr8_1:20532277-20532296 None:intergenic 15.0%
!! ACAAATTTCTATATACTTTA+GGG + chr8_1:20532356-20532375 Msa1184610:intron 15.0%
!! GATCTAATCTAAAGAATAAA+AGG - chr8_1:20531164-20531183 None:intergenic 20.0%
!! TATCACATAAAACACAATTT+GGG - chr8_1:20531479-20531498 None:intergenic 20.0%
!! TTATCACATAAAACACAATT+TGG - chr8_1:20531480-20531499 None:intergenic 20.0%
!!! TTTGAATTTGTTTATTCATC+CGG + chr8_1:20531601-20531620 Msa1184610:intron 20.0%
!!! AAAAAATCAAGGTTTTATCT+TGG + chr8_1:20531727-20531746 Msa1184610:intron 20.0%
!! GATAAAAGAAAAAAAAATGC+AGG - chr8_1:20531987-20532006 None:intergenic 20.0%
!! CTTACATATTTACATAGTTT+AGG - chr8_1:20532278-20532297 None:intergenic 20.0%
!! TAAATTGACAGAATTTATAC+AGG - chr8_1:20532305-20532324 None:intergenic 20.0%
!!! TAGTCTTTTATTAAGATGTT+AGG + chr8_1:20532323-20532342 Msa1184610:intron 20.0%
!!! AGTCTTTTATTAAGATGTTA+GGG + chr8_1:20532324-20532343 Msa1184610:intron 20.0%
!! GACAAATTTCTATATACTTT+AGG + chr8_1:20532355-20532374 Msa1184610:intron 20.0%
!! TTATTGTCTTTATACAGTTT+AGG + chr8_1:20532384-20532403 Msa1184610:intron 20.0%
!! AAACAAGAGTTATATGAAAA+GGG - chr8_1:20532814-20532833 None:intergenic 20.0%
!! AAAACAAGAGTTATATGAAA+AGG - chr8_1:20532815-20532834 None:intergenic 20.0%
!! AATCTTCCACAATAAAATTA+GGG + chr8_1:20533291-20533310 Msa1184610:CDS 20.0%
!!! TTCTTTGATTAAGTTTGTTA+AGG + chr8_1:20533697-20533716 Msa1184610:CDS 20.0%
!! AATAAAATGACTACTTTGTA+AGG + chr8_1:20534007-20534026 Msa1184610:three_prime_UTR 20.0%
!!! ATGATGCTTTAATTTCAATT+TGG + chr8_1:20534058-20534077 Msa1184610:three_prime_UTR 20.0%
!! AGTATTTGGAATGTCATAAT+TGG + chr8_1:20531039-20531058 Msa1184610:intron 25.0%
! TTCGAAAAACAATAGTATCT+TGG - chr8_1:20531186-20531205 None:intergenic 25.0%
! AAAAGCTATGACTTTATTGA+TGG - chr8_1:20531213-20531232 None:intergenic 25.0%
!!! ATAAAGTCATAGCTTTTTCT+TGG + chr8_1:20531216-20531235 Msa1184610:intron 25.0%
! TTCAGTGTAATTATATGTGT+CGG - chr8_1:20531355-20531374 None:intergenic 25.0%
! ATCACATAAAACACAATTTG+GGG - chr8_1:20531478-20531497 None:intergenic 25.0%
!! TTGTGTTTTATGTGATAACA+CGG + chr8_1:20531482-20531501 Msa1184610:intron 25.0%
! GGATGAATAAACAAATTCAA+AGG - chr8_1:20531602-20531621 None:intergenic 25.0%
! ATACCTAAAAAATAGTCTCT+CGG - chr8_1:20531813-20531832 None:intergenic 25.0%
!!! TTGAAGAGAGTTTTTGTTTA+TGG + chr8_1:20532414-20532433 Msa1184610:intron 25.0%
!!! TTGTTGTTATTTGTGTTCAA+TGG + chr8_1:20532867-20532886 Msa1184610:intron 25.0%
! AACAATTTGAGAATATTGGA+GGG - chr8_1:20533083-20533102 None:intergenic 25.0%
! GAATCTTCCACAATAAAATT+AGG + chr8_1:20533290-20533309 Msa1184610:CDS 25.0%
!! TCTAAACCCTAATTTTATTG+TGG - chr8_1:20533300-20533319 None:intergenic 25.0%
! TTATGAGAAAGATGAAAAAG+TGG + chr8_1:20533574-20533593 Msa1184610:CDS 25.0%
! GCTATTGATCATAATTTCTT+AGG + chr8_1:20533665-20533684 Msa1184610:CDS 25.0%
! ATTGATCATAATTTCTTAGG+CGG + chr8_1:20533668-20533687 Msa1184610:CDS 25.0%
!!! TAAGTTTGTTAAGGATGTTT+TGG + chr8_1:20533706-20533725 Msa1184610:CDS 25.0%
!! TTTTTCTTAAAGCAGATAGA+TGG + chr8_1:20533895-20533914 Msa1184610:three_prime_UTR 25.0%
!!! GATTACTAGAAAGTATGTTT+TGG + chr8_1:20534080-20534099 Msa1184610:three_prime_UTR 25.0%
ATTTGTTTATTCATCCGGAA+TGG + chr8_1:20531606-20531625 Msa1184610:intron 30.0%
AAATTAACCTATGACCATTC+CGG - chr8_1:20531623-20531642 None:intergenic 30.0%
! TAATTTTGCATGCAACGATA+TGG + chr8_1:20531637-20531656 Msa1184610:intron 30.0%
! AATTTTGCATGCAACGATAT+GGG + chr8_1:20531638-20531657 Msa1184610:intron 30.0%
! ATGACAGAATTTTCAACAAC+CGG + chr8_1:20531679-20531698 Msa1184610:intron 30.0%
GGATATAGTCGAAAAAATCA+AGG + chr8_1:20531716-20531735 Msa1184610:intron 30.0%
TTATCTTGGATGTAGAGTAT+AGG + chr8_1:20531741-20531760 Msa1184610:intron 30.0%
!!! AATCCGAGAGACTATTTTTT+AGG + chr8_1:20531807-20531826 Msa1184610:intron 30.0%
!!! GAGAGACTATTTTTTAGGTA+TGG + chr8_1:20531812-20531831 Msa1184610:intron 30.0%
TAGGGACTAAATTGAAGAAA+AGG - chr8_1:20532259-20532278 None:intergenic 30.0%
TTGAGTGATGGTTTATTCAT+TGG + chr8_1:20532439-20532458 Msa1184610:intron 30.0%
! GAACTGCATTTAAGATTCTT+AGG - chr8_1:20532545-20532564 None:intergenic 30.0%
GATTGAACTGAACAATATGT+AGG - chr8_1:20532647-20532666 None:intergenic 30.0%
!!! GTTCGATGTTATTGGTTTTA+TGG + chr8_1:20532679-20532698 Msa1184610:intron 30.0%
ACAATTTGAGAATATTGGAG+GGG - chr8_1:20533082-20533101 None:intergenic 30.0%
GAACAATTTGAGAATATTGG+AGG - chr8_1:20533084-20533103 None:intergenic 30.0%
AGGGAACAATTTGAGAATAT+TGG - chr8_1:20533087-20533106 None:intergenic 30.0%
! AAATTGTTCCCTTCTTTTCA+CGG + chr8_1:20533095-20533114 Msa1184610:CDS 30.0%
TCCATAAGAAACTTTCTATC+AGG - chr8_1:20533368-20533387 None:intergenic 30.0%
!!! ATGATGTAATGTTGGTTTTG+AGG + chr8_1:20533399-20533418 Msa1184610:CDS 30.0%
!!! TGATCTAGTTTTTGGATCTA+GGG + chr8_1:20533457-20533476 Msa1184610:CDS 30.0%
AATTGTTGGAGCTTAAGAAA+TGG + chr8_1:20533501-20533520 Msa1184610:CDS 30.0%
TGAAAAAGTGGATGAGAAAA+AGG + chr8_1:20533586-20533605 Msa1184610:CDS 30.0%
GAAAAAGTGGATGAGAAAAA+GGG + chr8_1:20533587-20533606 Msa1184610:CDS 30.0%
TGAGAAAGAATGATTATCAC+TGG + chr8_1:20533784-20533803 Msa1184610:exon 30.0%
ATCCTGACAAGAATGAATCT+TGG + chr8_1:20531257-20531276 Msa1184610:intron 35.0%
TCCTGACAAGAATGAATCTT+GGG + chr8_1:20531258-20531277 Msa1184610:intron 35.0%
! GTAATTATATGTGTCGGTGT+CGG - chr8_1:20531349-20531368 None:intergenic 35.0%
TCTCACATTATTAGCAGTGT+CGG + chr8_1:20531386-20531405 Msa1184610:intron 35.0%
! TGTTTTATGTGATAACACGG+CGG + chr8_1:20531485-20531504 Msa1184610:intron 35.0%
CACAATGAAGAAAAAGATGG+TGG - chr8_1:20531520-20531539 None:intergenic 35.0%
! CACCATCTTTTTCTTCATTG+TGG + chr8_1:20531518-20531537 Msa1184610:intron 35.0%
CTCCACAATGAAGAAAAAGA+TGG - chr8_1:20531523-20531542 None:intergenic 35.0%
! TCTTTTTCTTCATTGTGGAG+TGG + chr8_1:20531523-20531542 Msa1184610:intron 35.0%
TTTGGTTACAGGTCATGTTA+TGG + chr8_1:20531555-20531574 Msa1184610:intron 35.0%
TTACAGGTCATGTTATGGTT+AGG + chr8_1:20531560-20531579 Msa1184610:intron 35.0%
! ATGCAACGATATGGGTTTTA+TGG + chr8_1:20531646-20531665 Msa1184610:intron 35.0%
!! TATGGGTTTTATGGAAAGCT+AGG + chr8_1:20531655-20531674 Msa1184610:intron 35.0%
! TGACAGAATTTTCAACAACC+GGG + chr8_1:20531680-20531699 Msa1184610:intron 35.0%
CACTCATAGAACAAACAAGT+GGG - chr8_1:20531780-20531799 None:intergenic 35.0%
!!! ATTTTTTAGGTATGGTCGTG+CGG + chr8_1:20531820-20531839 Msa1184610:intron 35.0%
! GTCTTACTGTTGATGGAAAA+CGG + chr8_1:20531905-20531924 Msa1184610:intron 35.0%
! GAAAACGGTGTTTTTCTGTT+TGG + chr8_1:20531920-20531939 Msa1184610:intron 35.0%
! TTTTATCTGTCCCCTGTTAT+GGG + chr8_1:20532000-20532019 Msa1184610:intron 35.0%
ACCAAAAGAGCTAGCATTAA+GGG - chr8_1:20532075-20532094 None:intergenic 35.0%
AACCAAAAGAGCTAGCATTA+AGG - chr8_1:20532076-20532095 None:intergenic 35.0%
CGGGCATAGAAAAATACAAA+GGG - chr8_1:20532156-20532175 None:intergenic 35.0%
ACGGGCATAGAAAAATACAA+AGG - chr8_1:20532157-20532176 None:intergenic 35.0%
TGTAAGACACACAAAAACTC+GGG - chr8_1:20532186-20532205 None:intergenic 35.0%
GTGTAAGACACACAAAAACT+CGG - chr8_1:20532187-20532206 None:intergenic 35.0%
TTGTTTATGGACTTGAGTGA+TGG + chr8_1:20532427-20532446 Msa1184610:intron 35.0%
ACCAATAACATCGAACTAGA+GGG - chr8_1:20532675-20532694 None:intergenic 35.0%
AACCAATAACATCGAACTAG+AGG - chr8_1:20532676-20532695 None:intergenic 35.0%
GCACAGACTATCAAATTAGA+GGG - chr8_1:20532733-20532752 None:intergenic 35.0%
AGCACAGACTATCAAATTAG+AGG - chr8_1:20532734-20532753 None:intergenic 35.0%
AAACCAGAAAATGATGAGCA+GGG - chr8_1:20532784-20532803 None:intergenic 35.0%
AAAACCAGAAAATGATGAGC+AGG - chr8_1:20532785-20532804 None:intergenic 35.0%
ATACAGCTTCAGTAGCAAAA+TGG + chr8_1:20532890-20532909 Msa1184610:intron 35.0%
TATCTTCCAAAACAAGGTGA+GGG - chr8_1:20533160-20533179 None:intergenic 35.0%
ATATCTTCCAAAACAAGGTG+AGG - chr8_1:20533161-20533180 None:intergenic 35.0%
ATGCGATATCTTCCAAAACA+AGG - chr8_1:20533166-20533185 None:intergenic 35.0%
AATCTTCCGAAGACTACATT+CGG + chr8_1:20533246-20533265 Msa1184610:CDS 35.0%
AAGATTCTCGATCTTTCTGA+AGG - chr8_1:20533277-20533296 None:intergenic 35.0%
! AAATTAGGGTTTAGAGTCTC+CGG + chr8_1:20533305-20533324 Msa1184610:CDS 35.0%
TGGGTCCCAATAACAAATTA+TGG + chr8_1:20533339-20533358 Msa1184610:CDS 35.0%
!! GGCTGCCATAATTTGTTATT+GGG - chr8_1:20533347-20533366 None:intergenic 35.0%
!! AGGCTGCCATAATTTGTTAT+TGG - chr8_1:20533348-20533367 None:intergenic 35.0%
GCCTGATAGAAAGTTTCTTA+TGG + chr8_1:20533364-20533383 Msa1184610:CDS 35.0%
! ATCTGCTGATGATGTAATGT+TGG + chr8_1:20533391-20533410 Msa1184610:CDS 35.0%
AGGAAATTTCTTTCGTCGAA+TGG + chr8_1:20533419-20533438 Msa1184610:CDS 35.0%
CTAGATCCAAAAACTAGATC+AGG - chr8_1:20533458-20533477 None:intergenic 35.0%
!!! CTGATCTAGTTTTTGGATCT+AGG + chr8_1:20533456-20533475 Msa1184610:CDS 35.0%
! TAGGGTTTTTGTTCCTGTTT+TGG + chr8_1:20533475-20533494 Msa1184610:CDS 35.0%
TTATTCGTGTTCTGTTCTTG+TGG + chr8_1:20533550-20533569 Msa1184610:CDS 35.0%
!!! TTTGTTAAGGATGTTTTGGC+TGG + chr8_1:20533710-20533729 Msa1184610:CDS 35.0%
! TGAAAGATTGCACTGATGTA+GGG + chr8_1:20533818-20533837 Msa1184610:three_prime_UTR 35.0%
AATTCCGCGATGTATATTAG+CGG + chr8_1:20533868-20533887 Msa1184610:three_prime_UTR 35.0%
GATGGATAGATGAATAGACT+TGG + chr8_1:20533913-20533932 Msa1184610:three_prime_UTR 35.0%
GGCAATGAATGTTTGGTTAC+AGG + chr8_1:20531544-20531563 Msa1184610:intron 40.0%
TATTCATCCGGAATGGTCAT+AGG + chr8_1:20531613-20531632 Msa1184610:intron 40.0%
CCACTCATAGAACAAACAAG+TGG - chr8_1:20531781-20531800 None:intergenic 40.0%
CCACTTGTTTGTTCTATGAG+TGG + chr8_1:20531778-20531797 Msa1184610:intron 40.0%
GAGGCAGGACTATTCTATAT+TGG + chr8_1:20531863-20531882 Msa1184610:intron 40.0%
GTTCAAGGTCTTACTGTTGA+TGG + chr8_1:20531898-20531917 Msa1184610:intron 40.0%
AGCTCAGCAAACAAATAACG+AGG - chr8_1:20531956-20531975 None:intergenic 40.0%
! CTTTTATCTGTCCCCTGTTA+TGG + chr8_1:20531999-20532018 Msa1184610:intron 40.0%
AGTAGACAAACTAACACCCA+AGG - chr8_1:20532026-20532045 None:intergenic 40.0%
!! TCCCTTAATGCTAGCTCTTT+TGG + chr8_1:20532071-20532090 Msa1184610:intron 40.0%
!! AATGCTAGCTCTTTTGGTTG+TGG + chr8_1:20532077-20532096 Msa1184610:intron 40.0%
ACAAAAACTCGGGGACAAAA+CGG - chr8_1:20532176-20532195 None:intergenic 40.0%
GTAAGACACACAAAAACTCG+GGG - chr8_1:20532185-20532204 None:intergenic 40.0%
! TCCCTCTAGTTCGATGTTAT+TGG + chr8_1:20532671-20532690 Msa1184610:intron 40.0%
! CTAGGATTCCAGTTTTTGCT+TGG - chr8_1:20532953-20532972 None:intergenic 40.0%
CAAGCAAAAACTGGAATCCT+AGG + chr8_1:20532951-20532970 Msa1184610:CDS 40.0%
TTTCCAGAATCGTCTATCAG+TGG - chr8_1:20532978-20532997 None:intergenic 40.0%
TTTCAGTGCCGTGAAAAGAA+GGG - chr8_1:20533106-20533125 None:intergenic 40.0%
! TCAAGATTGGTTCTCGAACA+TGG + chr8_1:20533216-20533235 Msa1184610:CDS 40.0%
TGTAGTCTTCGGAAGATTGA+GGG - chr8_1:20533244-20533263 None:intergenic 40.0%
ATGTAGTCTTCGGAAGATTG+AGG - chr8_1:20533245-20533264 None:intergenic 40.0%
GCATTTCCGAATGTAGTCTT+CGG - chr8_1:20533255-20533274 None:intergenic 40.0%
! AATTAGGGTTTAGAGTCTCC+GGG + chr8_1:20533306-20533325 Msa1184610:CDS 40.0%
!!! GATGAGCCTGATCTAGTTTT+TGG + chr8_1:20533449-20533468 Msa1184610:CDS 40.0%
!! GGTTTTTGTTCCTGTTTTGG+AGG + chr8_1:20533478-20533497 Msa1184610:CDS 40.0%
!!! TCCTGTTTTGGAGGAATTGT+TGG + chr8_1:20533487-20533506 Msa1184610:CDS 40.0%
TCCAACAATTCCTCCAAAAC+AGG - chr8_1:20533491-20533510 None:intergenic 40.0%
GGAGCTTAAGAAATGGTTTG+AGG + chr8_1:20533508-20533527 Msa1184610:CDS 40.0%
!!! AAGGATGTTTTGGCTGGTTT+AGG + chr8_1:20533716-20533735 Msa1184610:CDS 40.0%
! GTGAAAGATTGCACTGATGT+AGG + chr8_1:20533817-20533836 Msa1184610:three_prime_UTR 40.0%
TGATGTAGGGAACAAATGAC+AGG + chr8_1:20533831-20533850 Msa1184610:three_prime_UTR 40.0%
TCTTCCGCTAATATACATCG+CGG - chr8_1:20533875-20533894 None:intergenic 40.0%
GCCCAAGATTCATTCTTGTC+AGG - chr8_1:20531262-20531281 None:intergenic 45.0%
GGAATGTCAGTGCTTACGAT+TGG - chr8_1:20531287-20531306 None:intergenic 45.0%
!! TCGTAAGCACTGACATTCCT+CGG + chr8_1:20531288-20531307 Msa1184610:intron 45.0%
ATCAGTGTCTGTGTCGTGTT+CGG + chr8_1:20531412-20531431 Msa1184610:intron 45.0%
ATGAAACACGGATACGGACA+CGG - chr8_1:20531441-20531460 None:intergenic 45.0%
TGGGCTATGAAACACGGATA+CGG - chr8_1:20531447-20531466 None:intergenic 45.0%
! CTCTCATGGGCTATGAAACA+CGG - chr8_1:20531453-20531472 None:intergenic 45.0%
ACAATTTGGGGAGCTCTCAT+GGG - chr8_1:20531466-20531485 None:intergenic 45.0%
TTTATGTGATAACACGGCGG+TGG + chr8_1:20531488-20531507 Msa1184610:intron 45.0%
GTGGAGTGGCAATGAATGTT+TGG + chr8_1:20531537-20531556 Msa1184610:intron 45.0%
TATCCTCAACACGAATAGCC+CGG - chr8_1:20531701-20531720 None:intergenic 45.0%
TGGATTTGCACTGCTGTTCA+AGG + chr8_1:20531883-20531902 Msa1184610:intron 45.0%
! TGTCTACTGTGTTGCTATGC+AGG + chr8_1:20532038-20532057 Msa1184610:intron 45.0%
!!! TTGGTTGTGGTGCTGCATTT+TGG + chr8_1:20532090-20532109 Msa1184610:intron 45.0%
!!! TGGTTGTGGTGCTGCATTTT+GGG + chr8_1:20532091-20532110 Msa1184610:intron 45.0%
!!! ATTTTGGGGTTAGGGTGGTT+TGG + chr8_1:20532106-20532125 Msa1184610:intron 45.0%
!!! TTTTGGGGTTAGGGTGGTTT+GGG + chr8_1:20532107-20532126 Msa1184610:intron 45.0%
CAAAAACTCGGGGACAAAAC+GGG - chr8_1:20532175-20532194 None:intergenic 45.0%
AGAAAATGATGAGCAGGGAG+AGG - chr8_1:20532779-20532798 None:intergenic 45.0%
! TCTCCCTGCTCATCATTTTC+TGG + chr8_1:20532778-20532797 Msa1184610:intron 45.0%
AATCGTCTATCAGTGGACCT+AGG - chr8_1:20532971-20532990 None:intergenic 45.0%
GGTGTAGAAAGCAAGTTCGT+TGG - chr8_1:20533036-20533055 None:intergenic 45.0%
GTTTCAGTGCCGTGAAAAGA+AGG - chr8_1:20533107-20533126 None:intergenic 45.0%
!! TCTACACCCTCACCTTGTTT+TGG + chr8_1:20533151-20533170 Msa1184610:CDS 45.0%
AATCTTGATGTCGACGACAG+CGG - chr8_1:20533204-20533223 None:intergenic 45.0%
!! TTGGCTGGTTTAGGTGATGT+TGG + chr8_1:20533725-20533744 Msa1184610:CDS 45.0%
! GCACTGACATTCCTCGGATT+AGG + chr8_1:20531294-20531313 Msa1184610:intron 50.0%
ACACGACACATGTCTGACAG+CGG - chr8_1:20531326-20531345 None:intergenic 50.0%
CACAATTTGGGGAGCTCTCA+TGG - chr8_1:20531467-20531486 None:intergenic 50.0%
TGATAACACGGCGGTGGAAT+CGG + chr8_1:20531494-20531513 Msa1184610:intron 50.0%
! TGGCGGTAGAAGAGGTTTTG+AGG + chr8_1:20531844-20531863 Msa1184610:intron 50.0%
!!! GGTAGAAGAGGTTTTGAGGC+AGG + chr8_1:20531848-20531867 Msa1184610:intron 50.0%
AACACCCAAGGCCCATAACA+GGG - chr8_1:20532014-20532033 None:intergenic 50.0%
TAACACCCAAGGCCCATAAC+AGG - chr8_1:20532015-20532034 None:intergenic 50.0%
!!! GGTTGTGGTGCTGCATTTTG+GGG + chr8_1:20532092-20532111 Msa1184610:intron 50.0%
!!! TGGTGCTGCATTTTGGGGTT+AGG + chr8_1:20532097-20532116 Msa1184610:intron 50.0%
!!! GGTGCTGCATTTTGGGGTTA+GGG + chr8_1:20532098-20532117 Msa1184610:intron 50.0%
AGCATGCATGCTGCAGACTA+AGG + chr8_1:20532478-20532497 Msa1184610:intron 50.0%
GCTGCAGACTAAGGAACTGA+TGG + chr8_1:20532487-20532506 Msa1184610:intron 50.0%
GCAGACTAAGGAACTGATGG+TGG + chr8_1:20532490-20532509 Msa1184610:intron 50.0%
!! GACTAAGGAACTGATGGTGG+TGG + chr8_1:20532493-20532512 Msa1184610:intron 50.0%
!! AGGAACTGATGGTGGTGGTA+AGG + chr8_1:20532498-20532517 Msa1184610:intron 50.0%
! TTGCTTGGTGACCAGCAACT+TGG - chr8_1:20532938-20532957 None:intergenic 50.0%
GCTGGTCACCAAGCAAAAAC+TGG + chr8_1:20532942-20532961 Msa1184610:CDS 50.0%
GGTCCACTGATAGACGATTC+TGG + chr8_1:20532972-20532991 Msa1184610:CDS 50.0%
GATGCGTGGATCGGAATAGA+GGG - chr8_1:20533060-20533079 None:intergenic 50.0%
AGAATATTGGAGGGGATGCG+TGG - chr8_1:20533074-20533093 None:intergenic 50.0%
! GACAGCGGGGTTAGTGTAAT+TGG - chr8_1:20533189-20533208 None:intergenic 50.0%
ATCTTGATGTCGACGACAGC+GGG - chr8_1:20533203-20533222 None:intergenic 50.0%
GCTGTCGTCGACATCAAGAT+TGG + chr8_1:20533203-20533222 Msa1184610:CDS 50.0%
GCTCACGTCGTTGATGCTAA+AGG + chr8_1:20533641-20533660 Msa1184610:CDS 50.0%
CACGACACATGTCTGACAGC+GGG - chr8_1:20531325-20531344 None:intergenic 55.0%
CAACCGGGCTATTCGTGTTG+AGG + chr8_1:20531695-20531714 Msa1184610:intron 55.0%
TTTAGGTATGGTCGTGCGGC+TGG + chr8_1:20531824-20531843 Msa1184610:intron 55.0%
TGTCCCCTGTTATGGGCCTT+GGG + chr8_1:20532007-20532026 Msa1184610:intron 55.0%
ACACCCAAGGCCCATAACAG+GGG - chr8_1:20532013-20532032 None:intergenic 55.0%
!!! GCTGCATTTTGGGGTTAGGG+TGG + chr8_1:20532101-20532120 Msa1184610:intron 55.0%
TGATGAGCAGGGAGAGGAGA+GGG - chr8_1:20532773-20532792 None:intergenic 55.0%
ATGATGAGCAGGGAGAGGAG+AGG - chr8_1:20532774-20532793 None:intergenic 55.0%
ATCCCAGACCACCAAGTTGC+TGG + chr8_1:20532924-20532943 Msa1184610:CDS 55.0%
! GACCAGCAACTTGGTGGTCT+GGG - chr8_1:20532929-20532948 None:intergenic 55.0%
! TGACCAGCAACTTGGTGGTC+TGG - chr8_1:20532930-20532949 None:intergenic 55.0%
! CTTGGTGACCAGCAACTTGG+TGG - chr8_1:20532935-20532954 None:intergenic 55.0%
CCTCGTTCGTCGTCTTGAAG+AGG - chr8_1:20533011-20533030 None:intergenic 55.0%
CCTCTTCAAGACGACGAACG+AGG + chr8_1:20533008-20533027 Msa1184610:CDS 55.0%
GGATGCGTGGATCGGAATAG+AGG - chr8_1:20533061-20533080 None:intergenic 55.0%
ATTGGAGGGGATGCGTGGAT+CGG - chr8_1:20533069-20533088 None:intergenic 55.0%
AGGGTGTAGACCAGAACCGT+CGG - chr8_1:20533141-20533160 None:intergenic 55.0%
TCTTGATGTCGACGACAGCG+GGG - chr8_1:20533202-20533221 None:intergenic 55.0%
! GGTTTAGAGTCTCCGGGTTG+AGG + chr8_1:20533312-20533331 Msa1184610:CDS 55.0%
CTGTCCCCTGTTATGGGCCT+TGG + chr8_1:20532006-20532025 Msa1184610:intron 60.0%
! GGGGTTAGGGTGGTTTGGGA+AGG + chr8_1:20532111-20532130 Msa1184610:intron 60.0%
GATGAGCAGGGAGAGGAGAG+GGG - chr8_1:20532772-20532791 None:intergenic 60.0%
GCGTGGATCGGAATAGAGGG+AGG - chr8_1:20533057-20533076 None:intergenic 60.0%
GAAACCGTTGACGCCTCCGA+CGG + chr8_1:20533122-20533141 Msa1184610:CDS 60.0%
!! AGAACCGTCGGAGGCGTCAA+CGG - chr8_1:20533129-20533148 None:intergenic 60.0%
GTGTAGACCAGAACCGTCGG+AGG - chr8_1:20533138-20533157 None:intergenic 60.0%
! AGTCTCCGGGTTGAGGTCTG+TGG + chr8_1:20533319-20533338 Msa1184610:CDS 60.0%
! GTCTCCGGGTTGAGGTCTGT+GGG + chr8_1:20533320-20533339 Msa1184610:CDS 60.0%
AGCGGGACACGCCTAATCCG+AGG - chr8_1:20531308-20531327 None:intergenic 65.0%
AGGTATGGTCGTGCGGCTGG+CGG + chr8_1:20531827-20531846 Msa1184610:intron 65.0%
GTTGACGCCTCCGACGGTTC+TGG + chr8_1:20533128-20533147 Msa1184610:CDS 65.0%
GGGACCCACAGACCTCAACC+CGG - chr8_1:20533327-20533346 None:intergenic 65.0%
CGTGCGGCTGGCGGTAGAAG+AGG + chr8_1:20531836-20531855 Msa1184610:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 20531032 20534112 20531032 ID=Msa1184610;Name=Msa1184610
chr8_1 mRNA 20531032 20534112 20531032 ID=Msa1184610-mRNA-1;Parent=Msa1184610;Name=Msa1184610-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0.5|0.5|1|1|2|316|302
chr8_1 exon 20531032 20531039 20531032 ID=Msa1184610-mRNA-1:exon:7884;Parent=Msa1184610-mRNA-1
chr8_1 exon 20532896 20534112 20532896 ID=Msa1184610-mRNA-1:exon:7885;Parent=Msa1184610-mRNA-1
chr8_1 CDS 20531032 20531039 20531032 ID=Msa1184610-mRNA-1:cds;Parent=Msa1184610-mRNA-1
chr8_1 CDS 20532896 20533796 20532896 ID=Msa1184610-mRNA-1:cds;Parent=Msa1184610-mRNA-1
chr8_1 three_prime_UTR 20533797 20534112 20533797 ID=Msa1184610-mRNA-1:three_prime_utr;Parent=Msa1184610-mRNA-1
Gene Sequence

>Msa1184610

ATGATTGACTTCAGTAGCAAAATGGTGAAGCTGAAAATCCCAGACCACCAAGTTGCTGGTCACCAAGCAAAAACTGGAATCCTAGGTCCACTGATAGACGATTCTGGAAAATTCTACAAACCTCTTCAAGACGACGAACGAGGTTCCAACGAACTTGCTTTCTACACCTCCCTCTATTCCGATCCACGCATCCCCTCCAATATTCTCAAATTGTTCCCTTCTTTTCACGGCACTGAAACCGTTGACGCCTCCGACGGTTCTGGTCTACACCCTCACCTTGTTTTGGAAGATATCGCATCCAATTACACTAACCCCGCTGTCGTCGACATCAAGATTGGTTCTCGAACATGGCACCCTCAATCTTCCGAAGACTACATTCGGAAATGCCTTCAGAAAGATCGAGAATCTTCCACAATAAAATTAGGGTTTAGAGTCTCCGGGTTGAGGTCTGTGGGTCCCAATAACAAATTATGGCAGCCTGATAGAAAGTTTCTTATGGATCTATCTGCTGATGATGTAATGTTGGTTTTGAGGAAATTTCTTTCGTCGAATGGAGATGATGATGAGCCTGATCTAGTTTTTGGATCTAGGGTTTTTGTTCCTGTTTTGGAGGAATTGTTGGAGCTTAAGAAATGGTTTGAGGTTCAAACTATTTTTCATTTTTATTCGTGTTCTGTTCTTGTGGTTTATGAGAAAGATGAAAAAGTGGATGAGAAAAAGGGTGCACGTGCTGTTGTTAAGCTTGTTGATTTTGCTCACGTCGTTGATGCTAAAGGTGCTATTGATCATAATTTCTTAGGCGGTCTTTGTTCTTTGATTAAGTTTGTTAAGGATGTTTTGGCTGGTTTAGGTGATGTTGGAAACAATGTTTCTAAAGATGAATCATCAAAGCTAAGTGAGAAAGAATGA
Protein sequence

>Msa1184610

MIDFSSKMVKLKIPDHQVAGHQAKTGILGPLIDDSGKFYKPLQDDERGSNELAFYTSLYSDPRIPSNILKLFPSFHGTETVDASDGSGLHPHLVLEDIASNYTNPAVVDIKIGSRTWHPQSSEDYIRKCLQKDRESSTIKLGFRVSGLRSVGPNNKLWQPDRKFLMDLSADDVMLVLRKFLSSNGDDDEPDLVFGSRVFVPVLEELLELKKWFEVQTIFHFYSCSVLVVYEKDEKVDEKKGARAVVKLVDFAHVVDAKGAIDHNFLGGLCSLIKFVKDVLAGLGDVGNNVSKDESSKLSEKE*