AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1185600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185600 MtrunA17_Chr8g0351271 93.567 342 21 1 1 341 1 342 0.0 632
Msa1185600 MtrunA17_Chr8g0351481 92.982 342 20 2 1 341 1 339 0.0 625
Msa1185600 MtrunA17_Chr8g0365421 47.097 310 159 4 5 311 77 384 3.33e-91 277
Msa1185600 MtrunA17_Chr6g0453201 46.302 311 161 5 5 311 12 320 1.17e-82 253
Msa1185600 MtrunA17_Chr6g0466321 45.192 312 163 6 5 311 20 328 7.85e-80 246
Msa1185600 MtrunA17_Chr6g0465501 44.872 312 164 6 5 311 20 328 4.58e-79 244
Msa1185600 MtrunA17_Chr7g0228011 47.436 312 156 6 5 311 9 317 2.48e-78 242
Msa1185600 MtrunA17_Chr6g0453191 46.127 284 147 5 5 284 12 293 1.34e-70 222
Msa1185600 MtrunA17_Chr4g0015091 30.746 335 192 9 2 307 4 327 3.80e-39 141
Msa1185600 MtrunA17_Chr4g0015051 32.738 336 185 10 2 307 4 328 1.22e-38 140
Msa1185600 MtrunA17_Chr7g0235021 30.149 335 192 9 2 306 4 326 9.04e-38 137
Msa1185600 MtrunA17_Chr4g0014951 33.333 336 183 12 2 307 4 328 2.08e-37 136
Msa1185600 MtrunA17_Chr4g0014971 32.344 337 187 9 1 307 3 328 5.50e-37 135
Msa1185600 MtrunA17_Chr4g0014691 29.619 341 190 10 1 307 3 327 7.57e-36 132
Msa1185600 MtrunA17_Chr4g0014541 29.619 341 190 10 1 307 3 327 1.49e-35 132
Msa1185600 MtrunA17_Chr4g0014931 32.544 338 183 11 2 307 4 328 4.66e-35 130
Msa1185600 MtrunA17_Chr2g0281281 32.018 228 134 6 5 223 2 217 2.66e-21 92.8
Msa1185600 MtrunA17_Chr2g0316731 31.481 216 135 5 13 223 36 243 3.97e-20 90.1
Msa1185600 MtrunA17_Chr2g0281301 29.956 227 140 5 5 223 15 230 1.94e-18 85.1
Msa1185600 MtrunA17_Chr4g0015001 29.060 234 127 7 104 307 5 229 1.02e-15 75.5
Msa1185600 MtrunA17_Chr4g0045041 24.412 340 200 11 24 321 309 633 1.07e-15 78.2
Msa1185600 MtrunA17_Chr4g0045061 27.459 244 139 8 103 312 385 624 6.90e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185600 AT1G23740.1 46.965 313 161 4 2 311 76 386 9.94e-89 271
Msa1185600 AT4G13010.1 29.851 335 195 9 2 307 4 327 2.61e-35 131
Msa1185600 AT3G15090.1 32.314 229 133 8 5 223 33 249 6.04e-20 90.1
Msa1185600 AT1G49670.1 25.595 336 197 12 24 321 309 629 1.50e-16 81.3
Msa1185600 AT1G49670.2 24.022 358 198 14 24 321 309 652 4.43e-14 73.6
Msa1185600 AT4G21580.3 26.638 229 139 7 5 223 2 211 2.50e-11 64.3

Find 84 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCAAATGAAAGACATTT+TGG 0.204648 8_1:+22873952 None:intergenic
GGAAAAGCTGAGACCATATT+TGG 0.216743 8_1:-22873428 Msa1185600:CDS
AACTCTCCTCTTTCCAAATA+TGG 0.217947 8_1:+22873415 None:intergenic
TCAAAATCATATTCACCTTT+TGG 0.241891 8_1:+22873376 None:intergenic
ACCATTGACTTGTGGTATAT+TGG 0.254521 8_1:+22873237 None:intergenic
GTGTAATTTAAGTTATTATA+TGG 0.267079 8_1:-22872941 Msa1185600:three_prime_UTR
TGCTTCACAAATTTCACTTT+TGG 0.284189 8_1:+22873754 None:intergenic
GAGGATAGTATAACAGTTAT+AGG 0.288464 8_1:-22875002 Msa1185600:five_prime_UTR
GACAGTTAGTGGTGAAATAT+TGG 0.300557 8_1:-22873449 Msa1185600:CDS
GGTATCAATCTCAGATAAAA+TGG 0.302019 8_1:+22873258 None:intergenic
TCACACATACATGATAGTTT+TGG 0.324075 8_1:-22874929 Msa1185600:five_prime_UTR
CATACAAGATTTCAACAGTA+TGG 0.367441 8_1:-22874043 Msa1185600:CDS
AACAGAATTTATGAGTCATT+AGG 0.374383 8_1:+22872890 None:intergenic
AAGTAATGTAATTTACCTTA+AGG 0.377254 8_1:-22873071 Msa1185600:three_prime_UTR
TATTCAAGTTTGACAGTTAG+TGG 0.383560 8_1:-22873460 Msa1185600:CDS
TGGCAGTTTGGTCCTTCAAT+TGG 0.387626 8_1:-22873824 Msa1185600:CDS
AGCAGGTGGTGTTGGCAGTT+TGG 0.390300 8_1:-22873836 Msa1185600:CDS
TATTGAAACTGGTAGAGCTT+GGG 0.391878 8_1:-22873329 Msa1185600:CDS
GCAAGAGGATAGAGCATTAG+AGG 0.404867 8_1:-22875021 Msa1185600:five_prime_UTR
ACCAAAATGTCTTTCATTTG+AGG 0.407754 8_1:-22873953 Msa1185600:CDS
GACTTTCTTTATGATACAAT+TGG 0.407929 8_1:-22873667 Msa1185600:intron
GTTGGTGGAGCAGGTGGTGT+TGG 0.408922 8_1:-22873844 Msa1185600:CDS
CAATTGGCTAAGCTTATGTT+TGG 0.410218 8_1:-22873808 Msa1185600:CDS
TAGGAAAGTCTCCTAGCTTA+AGG 0.413627 8_1:+22874747 None:intergenic
GTGAAATTTGTGAAGCAGTT+TGG 0.414239 8_1:-22873748 Msa1185600:CDS
ATGTTTGTGGTTGGTGGAGC+AGG 0.418587 8_1:-22873853 Msa1185600:CDS
GCCAATGTTCCAAGCTGCTT+TGG 0.435834 8_1:+22874015 None:intergenic
GTAGAGTGATGATGATGAAT+AGG 0.439245 8_1:+22873280 None:intergenic
GCAATTGAAGGATTCAAAAC+AGG 0.441078 8_1:-22873898 Msa1185600:CDS
GGAAGCTGCAAGTTTACCTT+TGG 0.441670 8_1:-22873932 Msa1185600:CDS
TCAAAGCAGCAGCATGAACT+TGG 0.447014 8_1:+22874693 None:intergenic
GCATTTGGTTATATTGAAAC+TGG 0.450862 8_1:-22873340 Msa1185600:CDS
GACATAGGTGATGAAGTCTA+TGG 0.451403 8_1:-22874069 Msa1185600:CDS
GAAAATGTGATTGATGCATT+TGG 0.451632 8_1:-22873355 Msa1185600:CDS
TTATGAAAATGCAGAAAGCT+TGG 0.470036 8_1:-22874799 Msa1185600:CDS
CATGAACTTGGACAAGTAGT+TGG 0.472618 8_1:+22874705 None:intergenic
GCTTGGTTCTATGAGGAGTA+TGG 0.473110 8_1:-22874782 Msa1185600:CDS
TTATGCAGAGGACATGGAAT+AGG 0.474727 8_1:+22874728 None:intergenic
GAGACAATGTTTGTGGTTGG+TGG 0.486435 8_1:-22873859 Msa1185600:CDS
ACATAAGCTTAGCCAATTGA+AGG 0.491472 8_1:+22873812 None:intergenic
GAGTGATGATGATGAATAGG+AGG 0.491561 8_1:+22873283 None:intergenic
CCTAGCTTAAGGACTTCCTT+AGG 0.494298 8_1:+22874758 None:intergenic
ATATTGAAACTGGTAGAGCT+TGG 0.498609 8_1:-22873330 Msa1185600:CDS
GCAGTTCAGACAGCAATTGA+AGG 0.503527 8_1:-22873910 Msa1185600:CDS
ACCAAAGCAGCTTGGAACAT+TGG 0.506549 8_1:-22874016 Msa1185600:CDS
CTATGAGGAGTATGGTCCTA+AGG 0.507415 8_1:-22874774 Msa1185600:CDS
AGTAAGTTGATGAGTGAGGA+AGG 0.519627 8_1:+22874894 None:intergenic
CTGAGACCATATTTGGAAAG+AGG 0.521526 8_1:-22873421 Msa1185600:CDS
AGCTGGTGACAATTTAATGT+TGG 0.524273 8_1:-22873170 Msa1185600:intron
GAAGGGTGAGACAATGTTTG+TGG 0.525326 8_1:-22873866 Msa1185600:CDS
ACTGCTCTTTGATGTGATGC+TGG 0.531182 8_1:+22873484 None:intergenic
TTACTATATTAATCACTAGC+TGG 0.536361 8_1:-22873187 Msa1185600:three_prime_UTR
TGATTGCAAGAAATCATTTG+TGG 0.538740 8_1:-22873545 Msa1185600:CDS
ATTGGCACAATTCATTGTTG+TGG 0.545838 8_1:-22873998 Msa1185600:CDS
CCTAAGGAAGTCCTTAAGCT+AGG 0.551849 8_1:-22874758 Msa1185600:CDS
ACCAATATACCACAAGTCAA+TGG 0.553808 8_1:-22873238 Msa1185600:CDS
GACATGGCTGGTGTTGTGAT+AGG 0.556375 8_1:-22874117 Msa1185600:CDS
AGTTGGTTATGCAGAGGACA+TGG 0.556609 8_1:+22874722 None:intergenic
AAAGCTGTGATTGATCCAAA+AGG 0.557716 8_1:-22873391 Msa1185600:CDS
ACACCAGCCATGTCACATCC+AGG 0.560683 8_1:+22874126 None:intergenic
CCTATGTTGTGTCCTCATGT+AGG 0.560889 8_1:-22873780 Msa1185600:CDS
TTTGTGGTTGGTGGAGCAGG+TGG 0.565693 8_1:-22873850 Msa1185600:CDS
GTTCCTGGATGTGACATGGC+TGG 0.567085 8_1:-22874129 Msa1185600:CDS
ATTGAAACTGGTAGAGCTTG+GGG 0.571714 8_1:-22873328 Msa1185600:CDS
AGTGATGATGATGAATAGGA+GGG 0.573864 8_1:+22873284 None:intergenic
GCTGGTGTTGTGATAGGAAA+AGG 0.582500 8_1:-22874111 Msa1185600:CDS
GCAGAAAGCTTGGTTCTATG+AGG 0.584465 8_1:-22874789 Msa1185600:CDS
GCACTCACAGGAAACTCAGA+TGG 0.585262 8_1:+22874632 None:intergenic
TTTGCAAAACCATTGACTTG+TGG 0.585776 8_1:+22873229 None:intergenic
GGTGAGACAATGTTTGTGGT+TGG 0.593803 8_1:-22873862 Msa1185600:CDS
GACTAAATATGAAGACATTG+AGG 0.595248 8_1:-22873698 Msa1185600:CDS
GCAGTTTGGTGCTGATAAAG+TGG 0.596691 8_1:-22873734 Msa1185600:CDS
ATATAGTAAGTTGATGAGTG+AGG 0.597266 8_1:+22874890 None:intergenic
CCTACATGAGGACACAACAT+AGG 0.602500 8_1:+22873780 None:intergenic
TGAATCCTATCGATTCAAAG+AGG 0.603465 8_1:-22874673 Msa1185600:CDS
ATACGCCTCTTTGAATCGAT+AGG 0.608807 8_1:+22874668 None:intergenic
ATTGCTGTCTGAACTGCCAA+AGG 0.610038 8_1:+22873916 None:intergenic
GGTGGTTCCTGGATGTGACA+TGG 0.619307 8_1:-22874133 Msa1185600:CDS
TAGCATCTATTGTAAGCAAG+AGG 0.619398 8_1:-22875036 Msa1185600:five_prime_UTR
GAGCAATAGTTGACATAACA+TGG 0.621358 8_1:-22873507 Msa1185600:CDS
ATTAATATAGTAACTACTTG+AGG 0.628995 8_1:+22873197 None:intergenic
AAAACTATCATGTATGTGTG+AGG 0.639036 8_1:+22874931 None:intergenic
ACAAGTAGTTGGTTATGCAG+AGG 0.650558 8_1:+22874716 None:intergenic
TTTGTGGTAGCAAAGAAAGA+CGG 0.668002 8_1:-22873529 Msa1185600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGGATAAACATTTTTTTAA+AGG - chr8_1:22873438-22873457 Msa1185600:CDS 15.0%
!! GTGTAATTAGTAATTTATAA+TGG + chr8_1:22874320-22874339 None:intergenic 15.0%
!!! GTGTAATTTAAGTTATTATA+TGG - chr8_1:22875000-22875019 Msa1185600:five_prime_UTR 15.0%
!!! ATGGATAAACATTTTTTTAA+AGG - chr8_1:22873438-22873457 Msa1185600:CDS 15.0%
!! GTGTAATTAGTAATTTATAA+TGG + chr8_1:22874320-22874339 None:intergenic 15.0%
!!! GTGTAATTTAAGTTATTATA+TGG - chr8_1:22875000-22875019 Msa1185600:five_prime_UTR 15.0%
!!! TAGAACTTTTTTCTCTTAAA+TGG + chr8_1:22873114-22873133 None:intergenic 20.0%
!!! ATACATTGATTTTTATATGC+AGG - chr8_1:22873787-22873806 Msa1185600:CDS 20.0%
!! AATGTCAAAAAATTTGACAT+AGG - chr8_1:22873857-22873876 Msa1185600:CDS 20.0%
!!! GATGATCTTATAAAATTTTC+AGG - chr8_1:22874373-22874392 Msa1185600:intron 20.0%
!! ATTAATATAGTAACTACTTG+AGG + chr8_1:22874747-22874766 None:intergenic 20.0%
!!! TGTTGGTTTTTTTTTTTTGA+AGG - chr8_1:22874788-22874807 Msa1185600:CDS 20.0%
!!! TTTTGAAGGAATTTTAATGT+TGG - chr8_1:22874802-22874821 Msa1185600:exon 20.0%
!! AAGTAATGTAATTTACCTTA+AGG - chr8_1:22874870-22874889 Msa1185600:five_prime_UTR 20.0%
!!! TAGAACTTTTTTCTCTTAAA+TGG + chr8_1:22873114-22873133 None:intergenic 20.0%
!!! ATACATTGATTTTTATATGC+AGG - chr8_1:22873787-22873806 Msa1185600:CDS 20.0%
!! AATGTCAAAAAATTTGACAT+AGG - chr8_1:22873857-22873876 Msa1185600:CDS 20.0%
!!! GATGATCTTATAAAATTTTC+AGG - chr8_1:22874373-22874392 Msa1185600:intron 20.0%
!! ATTAATATAGTAACTACTTG+AGG + chr8_1:22874747-22874766 None:intergenic 20.0%
!!! TGTTGGTTTTTTTTTTTTGA+AGG - chr8_1:22874788-22874807 Msa1185600:CDS 20.0%
!!! TTTTGAAGGAATTTTAATGT+TGG - chr8_1:22874802-22874821 Msa1185600:exon 20.0%
!! AAGTAATGTAATTTACCTTA+AGG - chr8_1:22874870-22874889 Msa1185600:five_prime_UTR 20.0%
!!! CTATCACATGATAATGTTTT+TGG - chr8_1:22872972-22872991 Msa1185600:intron 25.0%
!!! AACATTTTTTTAAAGGACCA+TGG - chr8_1:22873445-22873464 Msa1185600:CDS 25.0%
!! TTAATGGATATGTGAAGTTA+TGG - chr8_1:22873508-22873527 Msa1185600:CDS 25.0%
!!! AGAGAATTCTTTTCTGAAAA+AGG + chr8_1:22873671-22873690 None:intergenic 25.0%
!!! AAAACAGGAGATTTTAAGAA+GGG - chr8_1:22874058-22874077 Msa1185600:CDS 25.0%
! GACTTTCTTTATGATACAAT+TGG - chr8_1:22874274-22874293 Msa1185600:intron 25.0%
!!! AGTAATTTATAATGGATTCG+AGG + chr8_1:22874312-22874331 None:intergenic 25.0%
! TCGCATGTATTATAAAAAGT+TGG + chr8_1:22874345-22874364 None:intergenic 25.0%
! TTTATAATACATGCGAAAAG+TGG - chr8_1:22874349-22874368 Msa1185600:intron 25.0%
!! TCAAAATCATATTCACCTTT+TGG + chr8_1:22874568-22874587 None:intergenic 25.0%
! TTACTATATTAATCACTAGC+TGG - chr8_1:22874754-22874773 Msa1185600:CDS 25.0%
!!! GAATTTTAATGTTGGTTATG+AGG - chr8_1:22874810-22874829 Msa1185600:exon 25.0%
!! TACATTATTTTCACACCTTA+AGG + chr8_1:22874888-22874907 None:intergenic 25.0%
!!! CTATCACATGATAATGTTTT+TGG - chr8_1:22872972-22872991 Msa1185600:intron 25.0%
!!! AACATTTTTTTAAAGGACCA+TGG - chr8_1:22873445-22873464 Msa1185600:CDS 25.0%
!! TTAATGGATATGTGAAGTTA+TGG - chr8_1:22873508-22873527 Msa1185600:CDS 25.0%
!!! AGAGAATTCTTTTCTGAAAA+AGG + chr8_1:22873671-22873690 None:intergenic 25.0%
!!! AAAACAGGAGATTTTAAGAA+GGG - chr8_1:22874058-22874077 Msa1185600:CDS 25.0%
! GACTTTCTTTATGATACAAT+TGG - chr8_1:22874274-22874293 Msa1185600:intron 25.0%
!!! AGTAATTTATAATGGATTCG+AGG + chr8_1:22874312-22874331 None:intergenic 25.0%
! TCGCATGTATTATAAAAAGT+TGG + chr8_1:22874345-22874364 None:intergenic 25.0%
! TTTATAATACATGCGAAAAG+TGG - chr8_1:22874349-22874368 Msa1185600:intron 25.0%
!! TCAAAATCATATTCACCTTT+TGG + chr8_1:22874568-22874587 None:intergenic 25.0%
! TTACTATATTAATCACTAGC+TGG - chr8_1:22874754-22874773 Msa1185600:CDS 25.0%
!!! GAATTTTAATGTTGGTTATG+AGG - chr8_1:22874810-22874829 Msa1185600:exon 25.0%
!! TACATTATTTTCACACCTTA+AGG + chr8_1:22874888-22874907 None:intergenic 25.0%
GAGGATAGTATAACAGTTAT+AGG - chr8_1:22872939-22872958 Msa1185600:three_prime_UTR 30.0%
AAAACTATCATGTATGTGTG+AGG + chr8_1:22873013-22873032 None:intergenic 30.0%
! TCACACATACATGATAGTTT+TGG - chr8_1:22873012-22873031 Msa1185600:three_prime_UTR 30.0%
! CACACATACATGATAGTTTT+GGG - chr8_1:22873013-22873032 Msa1185600:three_prime_UTR 30.0%
!! ATATAGTAAGTTGATGAGTG+AGG + chr8_1:22873054-22873073 None:intergenic 30.0%
TTATGAAAATGCAGAAAGCT+TGG - chr8_1:22873142-22873161 Msa1185600:intron 30.0%
! TCCGTTTTATAAGTTTCGAA+AGG - chr8_1:22873411-22873430 Msa1185600:CDS 30.0%
CCCTTTCGAAACTTATAAAA+CGG + chr8_1:22873415-22873434 None:intergenic 30.0%
! CCGTTTTATAAGTTTCGAAA+GGG - chr8_1:22873412-22873431 Msa1185600:CDS 30.0%
ATAAGTTTCGAAAGGGATAA+TGG - chr8_1:22873419-22873438 Msa1185600:CDS 30.0%
TATAATATGCACTAGTTCCA+TGG + chr8_1:22873465-22873484 None:intergenic 30.0%
ATGGAACTAGTGCATATTAT+AGG - chr8_1:22873464-22873483 Msa1185600:CDS 30.0%
!!! CATTGATTTTTATATGCAGG+TGG - chr8_1:22873790-22873809 Msa1185600:CDS 30.0%
CATACAAGATTTCAACAGTA+TGG - chr8_1:22873898-22873917 Msa1185600:CDS 30.0%
ACCAAAATGTCTTTCATTTG+AGG - chr8_1:22873988-22874007 Msa1185600:CDS 30.0%
! TCCTCAAATGAAAGACATTT+TGG + chr8_1:22873992-22874011 None:intergenic 30.0%
!! CAAAACAGGAGATTTTAAGA+AGG - chr8_1:22874057-22874076 Msa1185600:CDS 30.0%
! TGCTTCACAAATTTCACTTT+TGG + chr8_1:22874190-22874209 None:intergenic 30.0%
GACTAAATATGAAGACATTG+AGG - chr8_1:22874243-22874262 Msa1185600:intron 30.0%
TGATTGCAAGAAATCATTTG+TGG - chr8_1:22874396-22874415 Msa1185600:intron 30.0%
! TATTCAAGTTTGACAGTTAG+TGG - chr8_1:22874481-22874500 Msa1185600:intron 30.0%
GAAAATGTGATTGATGCATT+TGG - chr8_1:22874586-22874605 Msa1185600:intron 30.0%
GCATTTGGTTATATTGAAAC+TGG - chr8_1:22874601-22874620 Msa1185600:intron 30.0%
GGTATCAATCTCAGATAAAA+TGG + chr8_1:22874686-22874705 None:intergenic 30.0%
ACTTATATTCCTAATGACTC+AGG - chr8_1:22875042-22875061 Msa1185600:five_prime_UTR 30.0%
CTTATATTCCTAATGACTCA+GGG - chr8_1:22875043-22875062 Msa1185600:five_prime_UTR 30.0%
GAGGATAGTATAACAGTTAT+AGG - chr8_1:22872939-22872958 Msa1185600:three_prime_UTR 30.0%
AAAACTATCATGTATGTGTG+AGG + chr8_1:22873013-22873032 None:intergenic 30.0%
! TCACACATACATGATAGTTT+TGG - chr8_1:22873012-22873031 Msa1185600:three_prime_UTR 30.0%
! CACACATACATGATAGTTTT+GGG - chr8_1:22873013-22873032 Msa1185600:three_prime_UTR 30.0%
!! ATATAGTAAGTTGATGAGTG+AGG + chr8_1:22873054-22873073 None:intergenic 30.0%
TTATGAAAATGCAGAAAGCT+TGG - chr8_1:22873142-22873161 Msa1185600:intron 30.0%
! TCCGTTTTATAAGTTTCGAA+AGG - chr8_1:22873411-22873430 Msa1185600:CDS 30.0%
CCCTTTCGAAACTTATAAAA+CGG + chr8_1:22873415-22873434 None:intergenic 30.0%
! CCGTTTTATAAGTTTCGAAA+GGG - chr8_1:22873412-22873431 Msa1185600:CDS 30.0%
ATAAGTTTCGAAAGGGATAA+TGG - chr8_1:22873419-22873438 Msa1185600:CDS 30.0%
TATAATATGCACTAGTTCCA+TGG + chr8_1:22873465-22873484 None:intergenic 30.0%
ATGGAACTAGTGCATATTAT+AGG - chr8_1:22873464-22873483 Msa1185600:CDS 30.0%
!!! CATTGATTTTTATATGCAGG+TGG - chr8_1:22873790-22873809 Msa1185600:CDS 30.0%
CATACAAGATTTCAACAGTA+TGG - chr8_1:22873898-22873917 Msa1185600:CDS 30.0%
ACCAAAATGTCTTTCATTTG+AGG - chr8_1:22873988-22874007 Msa1185600:CDS 30.0%
! TCCTCAAATGAAAGACATTT+TGG + chr8_1:22873992-22874011 None:intergenic 30.0%
!! CAAAACAGGAGATTTTAAGA+AGG - chr8_1:22874057-22874076 Msa1185600:CDS 30.0%
! TGCTTCACAAATTTCACTTT+TGG + chr8_1:22874190-22874209 None:intergenic 30.0%
GACTAAATATGAAGACATTG+AGG - chr8_1:22874243-22874262 Msa1185600:intron 30.0%
TGATTGCAAGAAATCATTTG+TGG - chr8_1:22874396-22874415 Msa1185600:intron 30.0%
! TATTCAAGTTTGACAGTTAG+TGG - chr8_1:22874481-22874500 Msa1185600:intron 30.0%
GAAAATGTGATTGATGCATT+TGG - chr8_1:22874586-22874605 Msa1185600:intron 30.0%
GCATTTGGTTATATTGAAAC+TGG - chr8_1:22874601-22874620 Msa1185600:intron 30.0%
GGTATCAATCTCAGATAAAA+TGG + chr8_1:22874686-22874705 None:intergenic 30.0%
TAGCATCTATTGTAAGCAAG+AGG - chr8_1:22872905-22872924 Msa1185600:three_prime_UTR 35.0%
!! TGAATCCTATCGATTCAAAG+AGG - chr8_1:22873268-22873287 Msa1185600:CDS 35.0%
GGAAACTCAGATGGAAAAAT+AGG + chr8_1:22873303-22873322 None:intergenic 35.0%
GTCAATCATGTGACAGTTAA+TGG - chr8_1:22873492-22873511 Msa1185600:CDS 35.0%
ATTGGCACAATTCATTGTTG+TGG - chr8_1:22873943-22873962 Msa1185600:CDS 35.0%
GCAATTGAAGGATTCAAAAC+AGG - chr8_1:22874043-22874062 Msa1185600:CDS 35.0%
ACATAAGCTTAGCCAATTGA+AGG + chr8_1:22874132-22874151 None:intergenic 35.0%
CAATTGGCTAAGCTTATGTT+TGG - chr8_1:22874133-22874152 Msa1185600:CDS 35.0%
GTGAAATTTGTGAAGCAGTT+TGG - chr8_1:22874193-22874212 Msa1185600:intron 35.0%
TTTGTGGTAGCAAAGAAAGA+CGG - chr8_1:22874412-22874431 Msa1185600:intron 35.0%
GAGCAATAGTTGACATAACA+TGG - chr8_1:22874434-22874453 Msa1185600:intron 35.0%
GACAGTTAGTGGTGAAATAT+TGG - chr8_1:22874492-22874511 Msa1185600:intron 35.0%
AACTCTCCTCTTTCCAAATA+TGG + chr8_1:22874529-22874548 None:intergenic 35.0%
AAAGCTGTGATTGATCCAAA+AGG - chr8_1:22874550-22874569 Msa1185600:intron 35.0%
ATATTGAAACTGGTAGAGCT+TGG - chr8_1:22874611-22874630 Msa1185600:intron 35.0%
TATTGAAACTGGTAGAGCTT+GGG - chr8_1:22874612-22874631 Msa1185600:intron 35.0%
! AGTGATGATGATGAATAGGA+GGG + chr8_1:22874660-22874679 None:intergenic 35.0%
! GTAGAGTGATGATGATGAAT+AGG + chr8_1:22874664-22874683 None:intergenic 35.0%
ACCAATATACCACAAGTCAA+TGG - chr8_1:22874703-22874722 Msa1185600:CDS 35.0%
!! ACCATTGACTTGTGGTATAT+TGG + chr8_1:22874707-22874726 None:intergenic 35.0%
!! TTTGCAAAACCATTGACTTG+TGG + chr8_1:22874715-22874734 None:intergenic 35.0%
AGCTGGTGACAATTTAATGT+TGG - chr8_1:22874771-22874790 Msa1185600:CDS 35.0%
TAGCATCTATTGTAAGCAAG+AGG - chr8_1:22872905-22872924 Msa1185600:three_prime_UTR 35.0%
!! TGAATCCTATCGATTCAAAG+AGG - chr8_1:22873268-22873287 Msa1185600:CDS 35.0%
GGAAACTCAGATGGAAAAAT+AGG + chr8_1:22873303-22873322 None:intergenic 35.0%
GTCAATCATGTGACAGTTAA+TGG - chr8_1:22873492-22873511 Msa1185600:CDS 35.0%
ATTGGCACAATTCATTGTTG+TGG - chr8_1:22873943-22873962 Msa1185600:CDS 35.0%
GCAATTGAAGGATTCAAAAC+AGG - chr8_1:22874043-22874062 Msa1185600:CDS 35.0%
ACATAAGCTTAGCCAATTGA+AGG + chr8_1:22874132-22874151 None:intergenic 35.0%
CAATTGGCTAAGCTTATGTT+TGG - chr8_1:22874133-22874152 Msa1185600:CDS 35.0%
GTGAAATTTGTGAAGCAGTT+TGG - chr8_1:22874193-22874212 Msa1185600:intron 35.0%
TTTGTGGTAGCAAAGAAAGA+CGG - chr8_1:22874412-22874431 Msa1185600:intron 35.0%
GAGCAATAGTTGACATAACA+TGG - chr8_1:22874434-22874453 Msa1185600:intron 35.0%
GACAGTTAGTGGTGAAATAT+TGG - chr8_1:22874492-22874511 Msa1185600:intron 35.0%
AACTCTCCTCTTTCCAAATA+TGG + chr8_1:22874529-22874548 None:intergenic 35.0%
AAAGCTGTGATTGATCCAAA+AGG - chr8_1:22874550-22874569 Msa1185600:intron 35.0%
ATATTGAAACTGGTAGAGCT+TGG - chr8_1:22874611-22874630 Msa1185600:intron 35.0%
TATTGAAACTGGTAGAGCTT+GGG - chr8_1:22874612-22874631 Msa1185600:intron 35.0%
! AGTGATGATGATGAATAGGA+GGG + chr8_1:22874660-22874679 None:intergenic 35.0%
! GTAGAGTGATGATGATGAAT+AGG + chr8_1:22874664-22874683 None:intergenic 35.0%
ACCAATATACCACAAGTCAA+TGG - chr8_1:22874703-22874722 Msa1185600:CDS 35.0%
!! ACCATTGACTTGTGGTATAT+TGG + chr8_1:22874707-22874726 None:intergenic 35.0%
!! TTTGCAAAACCATTGACTTG+TGG + chr8_1:22874715-22874734 None:intergenic 35.0%
AGCTGGTGACAATTTAATGT+TGG - chr8_1:22874771-22874790 Msa1185600:CDS 35.0%
!! AGTAAGTTGATGAGTGAGGA+AGG + chr8_1:22873050-22873069 None:intergenic 40.0%
TAGGAAAGTCTCCTAGCTTA+AGG + chr8_1:22873197-22873216 None:intergenic 40.0%
TTATGCAGAGGACATGGAAT+AGG + chr8_1:22873216-22873235 None:intergenic 40.0%
ACAAGTAGTTGGTTATGCAG+AGG + chr8_1:22873228-22873247 None:intergenic 40.0%
CATGAACTTGGACAAGTAGT+TGG + chr8_1:22873239-22873258 None:intergenic 40.0%
ATACGCCTCTTTGAATCGAT+AGG + chr8_1:22873276-22873295 None:intergenic 40.0%
TTAGAGTTTCAAGCACTCAC+AGG + chr8_1:22873324-22873343 None:intergenic 40.0%
!! AAGCAACTTGCTAGGAATCT+TGG + chr8_1:22873635-22873654 None:intergenic 40.0%
! TATGCAACAAGCAACTTGCT+AGG + chr8_1:22873643-22873662 None:intergenic 40.0%
! AGACACACAATCAGAAGTGT+CGG - chr8_1:22873728-22873747 Msa1185600:CDS 40.0%
! TTTTATATGCAGGTGGTTCC+TGG - chr8_1:22873797-22873816 Msa1185600:CDS 40.0%
GACATAGGTGATGAAGTCTA+TGG - chr8_1:22873872-22873891 Msa1185600:CDS 40.0%
ATGGAAAAACCAAAGCAGCT+TGG - chr8_1:22873917-22873936 Msa1185600:CDS 40.0%
GGAAAAGCTGAGACCATATT+TGG - chr8_1:22874513-22874532 Msa1185600:intron 40.0%
CTGAGACCATATTTGGAAAG+AGG - chr8_1:22874520-22874539 Msa1185600:intron 40.0%
ATTGAAACTGGTAGAGCTTG+GGG - chr8_1:22874613-22874632 Msa1185600:intron 40.0%
! GAGTGATGATGATGAATAGG+AGG + chr8_1:22874661-22874680 None:intergenic 40.0%
!! AGTAAGTTGATGAGTGAGGA+AGG + chr8_1:22873050-22873069 None:intergenic 40.0%
TAGGAAAGTCTCCTAGCTTA+AGG + chr8_1:22873197-22873216 None:intergenic 40.0%
TTATGCAGAGGACATGGAAT+AGG + chr8_1:22873216-22873235 None:intergenic 40.0%
ACAAGTAGTTGGTTATGCAG+AGG + chr8_1:22873228-22873247 None:intergenic 40.0%
CATGAACTTGGACAAGTAGT+TGG + chr8_1:22873239-22873258 None:intergenic 40.0%
ATACGCCTCTTTGAATCGAT+AGG + chr8_1:22873276-22873295 None:intergenic 40.0%
TTAGAGTTTCAAGCACTCAC+AGG + chr8_1:22873324-22873343 None:intergenic 40.0%
!! AAGCAACTTGCTAGGAATCT+TGG + chr8_1:22873635-22873654 None:intergenic 40.0%
! TATGCAACAAGCAACTTGCT+AGG + chr8_1:22873643-22873662 None:intergenic 40.0%
! AGACACACAATCAGAAGTGT+CGG - chr8_1:22873728-22873747 Msa1185600:CDS 40.0%
! TTTTATATGCAGGTGGTTCC+TGG - chr8_1:22873797-22873816 Msa1185600:CDS 40.0%
GACATAGGTGATGAAGTCTA+TGG - chr8_1:22873872-22873891 Msa1185600:CDS 40.0%
ATGGAAAAACCAAAGCAGCT+TGG - chr8_1:22873917-22873936 Msa1185600:CDS 40.0%
GGAAAAGCTGAGACCATATT+TGG - chr8_1:22874513-22874532 Msa1185600:intron 40.0%
CTGAGACCATATTTGGAAAG+AGG - chr8_1:22874520-22874539 Msa1185600:intron 40.0%
ATTGAAACTGGTAGAGCTTG+GGG - chr8_1:22874613-22874632 Msa1185600:intron 40.0%
! GAGTGATGATGATGAATAGG+AGG + chr8_1:22874661-22874680 None:intergenic 40.0%
! GCAAGAGGATAGAGCATTAG+AGG - chr8_1:22872920-22872939 Msa1185600:three_prime_UTR 45.0%
!! GCAGAAAGCTTGGTTCTATG+AGG - chr8_1:22873152-22873171 Msa1185600:intron 45.0%
!! GCTTGGTTCTATGAGGAGTA+TGG - chr8_1:22873159-22873178 Msa1185600:intron 45.0%
! CTATGAGGAGTATGGTCCTA+AGG - chr8_1:22873167-22873186 Msa1185600:intron 45.0%
CCTAGCTTAAGGACTTCCTT+AGG + chr8_1:22873186-22873205 None:intergenic 45.0%
CCTAAGGAAGTCCTTAAGCT+AGG - chr8_1:22873183-22873202 Msa1185600:three_prime_UTR 45.0%
AGTTGGTTATGCAGAGGACA+TGG + chr8_1:22873222-22873241 None:intergenic 45.0%
TCAAAGCAGCAGCATGAACT+TGG + chr8_1:22873251-22873270 None:intergenic 45.0%
GCATACGAAAGTTACCACTG+TGG + chr8_1:22873756-22873775 None:intergenic 45.0%
!! GCTGGTGTTGTGATAGGAAA+AGG - chr8_1:22873830-22873849 Msa1185600:CDS 45.0%
ACCAAAGCAGCTTGGAACAT+TGG - chr8_1:22873925-22873944 Msa1185600:CDS 45.0%
GGAAGCTGCAAGTTTACCTT+TGG - chr8_1:22874009-22874028 Msa1185600:CDS 45.0%
ATTGCTGTCTGAACTGCCAA+AGG + chr8_1:22874028-22874047 None:intergenic 45.0%
GCAGTTCAGACAGCAATTGA+AGG - chr8_1:22874031-22874050 Msa1185600:CDS 45.0%
GAAGGGTGAGACAATGTTTG+TGG - chr8_1:22874075-22874094 Msa1185600:CDS 45.0%
GGTGAGACAATGTTTGTGGT+TGG - chr8_1:22874079-22874098 Msa1185600:CDS 45.0%
GAGACAATGTTTGTGGTTGG+TGG - chr8_1:22874082-22874101 Msa1185600:CDS 45.0%
TGGCAGTTTGGTCCTTCAAT+TGG - chr8_1:22874117-22874136 Msa1185600:CDS 45.0%
CCTACATGAGGACACAACAT+AGG + chr8_1:22874164-22874183 None:intergenic 45.0%
CCTATGTTGTGTCCTCATGT+AGG - chr8_1:22874161-22874180 Msa1185600:intron 45.0%
!!! CACTTTTGGAGTCCTACATG+AGG + chr8_1:22874176-22874195 None:intergenic 45.0%
!! GCAGTTTGGTGCTGATAAAG+TGG - chr8_1:22874207-22874226 Msa1185600:intron 45.0%
ACTGCTCTTTGATGTGATGC+TGG + chr8_1:22874460-22874479 None:intergenic 45.0%
! GCAAGAGGATAGAGCATTAG+AGG - chr8_1:22872920-22872939 Msa1185600:three_prime_UTR 45.0%
!! GCAGAAAGCTTGGTTCTATG+AGG - chr8_1:22873152-22873171 Msa1185600:intron 45.0%
!! GCTTGGTTCTATGAGGAGTA+TGG - chr8_1:22873159-22873178 Msa1185600:intron 45.0%
! CTATGAGGAGTATGGTCCTA+AGG - chr8_1:22873167-22873186 Msa1185600:intron 45.0%
CCTAGCTTAAGGACTTCCTT+AGG + chr8_1:22873186-22873205 None:intergenic 45.0%
CCTAAGGAAGTCCTTAAGCT+AGG - chr8_1:22873183-22873202 Msa1185600:three_prime_UTR 45.0%
AGTTGGTTATGCAGAGGACA+TGG + chr8_1:22873222-22873241 None:intergenic 45.0%
TCAAAGCAGCAGCATGAACT+TGG + chr8_1:22873251-22873270 None:intergenic 45.0%
GCATACGAAAGTTACCACTG+TGG + chr8_1:22873756-22873775 None:intergenic 45.0%
!! GCTGGTGTTGTGATAGGAAA+AGG - chr8_1:22873830-22873849 Msa1185600:CDS 45.0%
ACCAAAGCAGCTTGGAACAT+TGG - chr8_1:22873925-22873944 Msa1185600:CDS 45.0%
GGAAGCTGCAAGTTTACCTT+TGG - chr8_1:22874009-22874028 Msa1185600:CDS 45.0%
ATTGCTGTCTGAACTGCCAA+AGG + chr8_1:22874028-22874047 None:intergenic 45.0%
GCAGTTCAGACAGCAATTGA+AGG - chr8_1:22874031-22874050 Msa1185600:CDS 45.0%
GAAGGGTGAGACAATGTTTG+TGG - chr8_1:22874075-22874094 Msa1185600:CDS 45.0%
GGTGAGACAATGTTTGTGGT+TGG - chr8_1:22874079-22874098 Msa1185600:CDS 45.0%
GAGACAATGTTTGTGGTTGG+TGG - chr8_1:22874082-22874101 Msa1185600:CDS 45.0%
TGGCAGTTTGGTCCTTCAAT+TGG - chr8_1:22874117-22874136 Msa1185600:CDS 45.0%
CCTACATGAGGACACAACAT+AGG + chr8_1:22874164-22874183 None:intergenic 45.0%
CCTATGTTGTGTCCTCATGT+AGG - chr8_1:22874161-22874180 Msa1185600:intron 45.0%
!!! CACTTTTGGAGTCCTACATG+AGG + chr8_1:22874176-22874195 None:intergenic 45.0%
!! GCAGTTTGGTGCTGATAAAG+TGG - chr8_1:22874207-22874226 Msa1185600:intron 45.0%
ACTGCTCTTTGATGTGATGC+TGG + chr8_1:22874460-22874479 None:intergenic 45.0%
GCACTCACAGGAAACTCAGA+TGG + chr8_1:22873312-22873331 None:intergenic 50.0%
!! GACATGGCTGGTGTTGTGAT+AGG - chr8_1:22873824-22873843 Msa1185600:CDS 50.0%
GCCAATGTTCCAAGCTGCTT+TGG + chr8_1:22873929-22873948 None:intergenic 50.0%
!! ATGTTTGTGGTTGGTGGAGC+AGG - chr8_1:22874088-22874107 Msa1185600:CDS 50.0%
GCACTCACAGGAAACTCAGA+TGG + chr8_1:22873312-22873331 None:intergenic 50.0%
!! GACATGGCTGGTGTTGTGAT+AGG - chr8_1:22873824-22873843 Msa1185600:CDS 50.0%
GCCAATGTTCCAAGCTGCTT+TGG + chr8_1:22873929-22873948 None:intergenic 50.0%
!! ATGTTTGTGGTTGGTGGAGC+AGG - chr8_1:22874088-22874107 Msa1185600:CDS 50.0%
GGTGGTTCCTGGATGTGACA+TGG - chr8_1:22873808-22873827 Msa1185600:CDS 55.0%
GTTCCTGGATGTGACATGGC+TGG - chr8_1:22873812-22873831 Msa1185600:CDS 55.0%
ACACCAGCCATGTCACATCC+AGG + chr8_1:22873818-22873837 None:intergenic 55.0%
!! TTTGTGGTTGGTGGAGCAGG+TGG - chr8_1:22874091-22874110 Msa1185600:CDS 55.0%
!! AGCAGGTGGTGTTGGCAGTT+TGG - chr8_1:22874105-22874124 Msa1185600:CDS 55.0%
GGTGGTTCCTGGATGTGACA+TGG - chr8_1:22873808-22873827 Msa1185600:CDS 55.0%
GTTCCTGGATGTGACATGGC+TGG - chr8_1:22873812-22873831 Msa1185600:CDS 55.0%
ACACCAGCCATGTCACATCC+AGG + chr8_1:22873818-22873837 None:intergenic 55.0%
!! TTTGTGGTTGGTGGAGCAGG+TGG - chr8_1:22874091-22874110 Msa1185600:CDS 55.0%
!! AGCAGGTGGTGTTGGCAGTT+TGG - chr8_1:22874105-22874124 Msa1185600:CDS 55.0%
!! CAGAAGTGTCGGTGCCACAG+TGG - chr8_1:22873739-22873758 Msa1185600:CDS 60.0%
! GTTGGTGGAGCAGGTGGTGT+TGG - chr8_1:22874097-22874116 Msa1185600:CDS 60.0%
!! CAGAAGTGTCGGTGCCACAG+TGG - chr8_1:22873739-22873758 Msa1185600:CDS 60.0%
! GTTGGTGGAGCAGGTGGTGT+TGG - chr8_1:22874097-22874116 Msa1185600:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 22872901 22875062 22872901 ID=Msa1185600;Name=Msa1185600
chr8_1 mRNA 22872901 22875062 22872901 ID=Msa1185600-mRNA-1;Parent=Msa1185600;Name=Msa1185600-mRNA-1;_AED=0.05;_eAED=0.05;_QI=243|1|1|1|0.5|0.4|5|236|341
chr8_1 exon 22872901 22872976 22872901 ID=Msa1185600-mRNA-1:exon:8384;Parent=Msa1185600-mRNA-1
chr8_1 exon 22873011 22873131 22873011 ID=Msa1185600-mRNA-1:exon:8383;Parent=Msa1185600-mRNA-1
chr8_1 exon 22873171 22873568 22873171 ID=Msa1185600-mRNA-1:exon:8382;Parent=Msa1185600-mRNA-1
chr8_1 exon 22873668 22874154 22873668 ID=Msa1185600-mRNA-1:exon:8381;Parent=Msa1185600-mRNA-1
chr8_1 exon 22874640 22875062 22874640 ID=Msa1185600-mRNA-1:exon:8380;Parent=Msa1185600-mRNA-1
chr8_1 five_prime_UTR 22874820 22875062 22874820 ID=Msa1185600-mRNA-1:five_prime_utr;Parent=Msa1185600-mRNA-1
chr8_1 CDS 22874640 22874819 22874640 ID=Msa1185600-mRNA-1:cds;Parent=Msa1185600-mRNA-1
chr8_1 CDS 22873668 22874154 22873668 ID=Msa1185600-mRNA-1:cds;Parent=Msa1185600-mRNA-1
chr8_1 CDS 22873210 22873568 22873210 ID=Msa1185600-mRNA-1:cds;Parent=Msa1185600-mRNA-1
chr8_1 three_prime_UTR 22873171 22873209 22873171 ID=Msa1185600-mRNA-1:three_prime_utr;Parent=Msa1185600-mRNA-1
chr8_1 three_prime_UTR 22873011 22873131 22873011 ID=Msa1185600-mRNA-1:three_prime_utr;Parent=Msa1185600-mRNA-1
chr8_1 three_prime_UTR 22872901 22872976 22872901 ID=Msa1185600-mRNA-1:three_prime_utr;Parent=Msa1185600-mRNA-1
Gene Sequence

>Msa1185600

ATGAAAATGCAGAAAGCTTGGTTCTATGAGGAGTATGGTCCTAAGGAAGTCCTTAAGCTAGGAGACTTTCCTATTCCATGTCCTCTGCATAACCAACTACTTGTCCAAGTTCATGCTGCTGCTTTGAATCCTATCGATTCAAAGAGGCGTATGCGTCCTATTTTTCCATCTGAGTTTCCTGTGGTTCCTGGATGTGACATGGCTGGTGTTGTGATAGGAAAAGGAATTAATGTCAAAAAATTTGACATAGGTGATGAAGTCTATGGAAACATACAAGATTTCAACAGTATGGAAAAACCAAAGCAGCTTGGAACATTGGCACAATTCATTGTTGTGGAAGAGAAGTTAGTTGCAAGAAAACCAAAATGTCTTTCATTTGAGGAAGCTGCAAGTTTACCTTTGGCAGTTCAGACAGCAATTGAAGGATTCAAAACAGGAGATTTTAAGAAGGGTGAGACAATGTTTGTGGTTGGTGGAGCAGGTGGTGTTGGCAGTTTGGTCCTTCAATTGGCTAAGCTTATGTTTGGAGCATCCTATGTTGTGTCCTCATGTAGGACTCCAAAAGTGAAATTTGTGAAGCAGTTTGGTGCTGATAAAGTGGTTGACTATACTAAGACTAAATATGAAGACATTGAGGACAAATTTGACTTTCTTTATGATACAATTGGTGATTGCAAGAAATCATTTGTGGTAGCAAAGAAAGACGGAGCAATAGTTGACATAACATGGCCAGCATCACATCAAAGAGCAGTTTATTCAAGTTTGACAGTTAGTGGTGAAATATTGGAAAAGCTGAGACCATATTTGGAAAGAGGAGAGTTAAAAGCTGTGATTGATCCAAAAGGTGAATATGATTTTGAAAATGTGATTGATGCATTTGGTTATATTGAAACTGGTAGAGCTTGGGGTAAAGTTGTTGTCACATGTTTCCCTCCTATTCATCATCATCACTCTACCATTTTATCTGAGATTGATACCAATATACCACAAGTCAATGGTTTTGCAAAAGATCTTTGCCTCAAGTAG
Protein sequence

>Msa1185600

MKMQKAWFYEEYGPKEVLKLGDFPIPCPLHNQLLVQVHAAALNPIDSKRRMRPIFPSEFPVVPGCDMAGVVIGKGINVKKFDIGDEVYGNIQDFNSMEKPKQLGTLAQFIVVEEKLVARKPKCLSFEEAASLPLAVQTAIEGFKTGDFKKGETMFVVGGAGGVGSLVLQLAKLMFGASYVVSSCRTPKVKFVKQFGADKVVDYTKTKYEDIEDKFDFLYDTIGDCKKSFVVAKKDGAIVDITWPASHQRAVYSSLTVSGEILEKLRPYLERGELKAVIDPKGEYDFENVIDAFGYIETGRAWGKVVVTCFPPIHHHHSTILSEIDTNIPQVNGFAKDLCLK*