AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1280910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1280910 MtrunA17_Chr8g0351271 94.152 342 19 1 1 341 1 342 0.0 634
Msa1280910 MtrunA17_Chr8g0351481 93.275 342 19 3 1 341 1 339 0.0 622
Msa1280910 MtrunA17_Chr8g0365421 46.452 310 161 4 5 311 77 384 9.52e-91 276
Msa1280910 MtrunA17_Chr6g0453201 46.945 311 159 5 5 311 12 320 5.06e-84 257
Msa1280910 MtrunA17_Chr6g0466321 45.513 312 162 6 5 311 20 328 3.38e-80 247
Msa1280910 MtrunA17_Chr7g0228011 48.397 312 153 6 5 311 9 317 1.20e-79 246
Msa1280910 MtrunA17_Chr6g0465501 45.338 311 162 6 6 311 21 328 1.99e-79 245
Msa1280910 MtrunA17_Chr6g0453191 46.479 284 146 5 5 284 12 293 4.78e-72 226
Msa1280910 MtrunA17_Chr4g0015091 31.045 335 191 9 2 307 4 327 3.69e-39 141
Msa1280910 MtrunA17_Chr7g0235021 30.746 335 190 9 2 306 4 326 1.77e-38 139
Msa1280910 MtrunA17_Chr4g0015051 32.440 336 186 10 2 307 4 328 1.89e-38 139
Msa1280910 MtrunA17_Chr4g0014951 33.333 336 183 12 2 307 4 328 1.45e-37 137
Msa1280910 MtrunA17_Chr4g0014971 32.344 337 187 9 1 307 3 328 1.73e-37 137
Msa1280910 MtrunA17_Chr4g0014931 32.840 338 182 10 2 307 4 328 2.13e-36 134
Msa1280910 MtrunA17_Chr4g0014691 30.205 341 188 10 1 307 3 327 2.71e-36 134
Msa1280910 MtrunA17_Chr4g0014541 30.205 341 188 10 1 307 3 327 5.10e-36 133
Msa1280910 MtrunA17_Chr2g0281281 33.186 226 134 6 5 225 2 215 4.67e-22 95.1
Msa1280910 MtrunA17_Chr2g0281301 31.111 225 140 5 5 225 15 228 2.01e-19 87.8
Msa1280910 MtrunA17_Chr2g0316731 30.556 216 137 5 13 223 36 243 3.77e-19 87.4
Msa1280910 MtrunA17_Chr4g0015001 29.487 234 126 7 104 307 5 229 6.26e-16 76.3
Msa1280910 MtrunA17_Chr4g0045041 24.706 340 199 13 24 321 309 633 3.64e-15 76.6
Msa1280910 MtrunA17_Chr4g0045061 29.091 220 119 9 127 312 408 624 1.70e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1280910 AT1G23740.1 45.687 313 165 4 2 311 76 386 3.31e-87 268
Msa1280910 AT4G13010.1 30.060 336 193 10 2 307 4 327 2.53e-34 129
Msa1280910 AT3G15090.1 30.531 226 141 7 5 223 33 249 1.89e-19 88.6
Msa1280910 AT1G49670.1 26.786 336 193 14 24 321 309 629 1.92e-17 84.0
Msa1280910 AT1G49670.2 25.072 347 208 13 24 321 309 652 4.42e-15 76.6
Msa1280910 AT4G21580.3 25.877 228 142 6 5 223 2 211 2.26e-11 64.3

Find 83 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCAAATGAAAGACATTT+TGG 0.204648 8_3:-19897183 None:intergenic
GGAAAAGCTGAGACCATATT+TGG 0.216743 8_3:+19897708 Msa1280910:CDS
AACTCTCCTCTTTCCAAATA+TGG 0.217947 8_3:-19897721 None:intergenic
TCAAAATCATATTCACCTTT+TGG 0.241891 8_3:-19897760 None:intergenic
ACCATTGACTTGTGGTATAT+TGG 0.254521 8_3:-19897899 None:intergenic
GTGTAATTTAAGTTATTATA+TGG 0.267079 8_3:+19898195 Msa1280910:three_prime_UTR
GAGGATAGTATAACAGTTAT+AGG 0.271030 8_3:+19896136 Msa1280910:five_prime_UTR
TGCTTCACAAATTTCACTTT+TGG 0.284189 8_3:-19897381 None:intergenic
GACAGTTAGTGGTGAAATAT+TGG 0.300557 8_3:+19897687 Msa1280910:CDS
GGTATCAATCTCAGATAAAA+TGG 0.302019 8_3:-19897878 None:intergenic
TCACACATACATGATAGTTT+TGG 0.324075 8_3:+19896209 Msa1280910:five_prime_UTR
CAATTGGCTAAACTTATGTT+TGG 0.352740 8_3:+19897327 Msa1280910:CDS
TCAACTTACTATATATATCT+TGG 0.367060 8_3:+19896245 Msa1280910:five_prime_UTR
CATACAAGATTTCAACAGTA+TGG 0.367441 8_3:+19897092 Msa1280910:CDS
AACAGAATTTATGAGTCATT+AGG 0.374383 8_3:-19898246 None:intergenic
AAGTAATGTAATTTACCTTA+AGG 0.377254 8_3:+19898065 Msa1280910:three_prime_UTR
TGGAACTTTGGTCCTTCAAT+TGG 0.377358 8_3:+19897311 Msa1280910:CDS
TATTCAAGTTTGACAGTTAG+TGG 0.383560 8_3:+19897676 Msa1280910:CDS
GCATTTGGTTATATTGAAAC+TGG 0.385666 8_3:+19897796 Msa1280910:CDS
TATTGAAACTGGAAGAGCTT+GGG 0.391878 8_3:+19897807 Msa1280910:CDS
GCCAATGTTCCAAGCTGCTT+TGG 0.392306 8_3:-19897120 None:intergenic
ACCAAAATGTCTTTCATTTG+AGG 0.407754 8_3:+19897182 Msa1280910:CDS
TAGGAAAGTCTCCTAGCTTA+AGG 0.413627 8_3:-19896379 None:intergenic
GTGAAATTTGTGAAGCAGTT+TGG 0.414239 8_3:+19897387 Msa1280910:CDS
AAACTTGAATAAACTGCTCT+TGG 0.414663 8_3:-19897664 None:intergenic
ATGTTTGTGGTTGGTGGAGC+AGG 0.418587 8_3:+19897282 Msa1280910:CDS
GTTGGTGGAGCAGGTGGTGT+TGG 0.435659 8_3:+19897291 Msa1280910:CDS
GTAGAGTGATGATGATGAAT+AGG 0.439245 8_3:-19897856 None:intergenic
GCAATTGAAGGATTCAAAAC+AGG 0.441078 8_3:+19897237 Msa1280910:CDS
GGAAGCTGCAAGTTTACCTT+TGG 0.441670 8_3:+19897203 Msa1280910:CDS
TCAAAGCAGCAGCATGAACT+TGG 0.447014 8_3:-19896433 None:intergenic
GACATAGGTGATGAAGTCTA+TGG 0.451403 8_3:+19897066 Msa1280910:CDS
GAAAATGTGATTGATGCATT+TGG 0.451632 8_3:+19897781 Msa1280910:CDS
ACATAAGTTTAGCCAATTGA+AGG 0.458515 8_3:-19897323 None:intergenic
AGCAGGTGGTGTTGGAACTT+TGG 0.465960 8_3:+19897299 Msa1280910:CDS
CATGAACTTGGACAAGTAGT+TGG 0.472618 8_3:-19896421 None:intergenic
GCTTGGTTCTATGAGGAGTA+TGG 0.473110 8_3:+19896344 Msa1280910:CDS
TTATGCAGAGGACATGGAAT+AGG 0.474727 8_3:-19896398 None:intergenic
ACTGCTCTTGGATGTGATGC+TGG 0.478888 8_3:-19897652 None:intergenic
GAGACAATGTTTGTGGTTGG+TGG 0.486435 8_3:+19897276 Msa1280910:CDS
GAGTGATGATGATGAATAGG+AGG 0.491561 8_3:-19897853 None:intergenic
CCTAGCTTAAGGACTTCCTT+AGG 0.494298 8_3:-19896368 None:intergenic
AACTTTCTTTATGATACAGT+TGG 0.494702 8_3:+19897468 Msa1280910:CDS
GCAAGAGGATAGAACATAAG+AGG 0.496716 8_3:+19896117 Msa1280910:five_prime_UTR
GCAGTTCAGACAGCAATTGA+AGG 0.503527 8_3:+19897225 Msa1280910:CDS
ACCAAAGCAGCTTGGAACAT+TGG 0.506549 8_3:+19897119 Msa1280910:CDS
CTATGAGGAGTATGGTCCTA+AGG 0.507415 8_3:+19896352 Msa1280910:CDS
ATATGAAAATGCAGAAAGCT+TGG 0.507566 8_3:+19896327 Msa1280910:exon
GACTAAATATGAAGAAATTG+AGG 0.507833 8_3:+19897437 Msa1280910:CDS
CTGAGACCATATTTGGAAAG+AGG 0.521526 8_3:+19897715 Msa1280910:CDS
AGCTGGTGACAATTTAATGT+TGG 0.524273 8_3:+19897966 Msa1280910:three_prime_UTR
GAAGGGTGAGACAATGTTTG+TGG 0.525326 8_3:+19897269 Msa1280910:CDS
TTACTATATTAATCACTAGC+TGG 0.536361 8_3:+19897949 Msa1280910:three_prime_UTR
TGATTGCAAGAAATCATTTG+TGG 0.538740 8_3:+19897591 Msa1280910:CDS
ATATTGAAACTGGAAGAGCT+TGG 0.539768 8_3:+19897806 Msa1280910:CDS
AGTGATGATGATGAATAGGA+GGG 0.543361 8_3:-19897852 None:intergenic
AAAGCAGTGATTGATCCAAA+AGG 0.548470 8_3:+19897745 Msa1280910:CDS
CCTAAGGAAGTCCTTAAGCT+AGG 0.551849 8_3:+19896368 Msa1280910:CDS
ACCAATATACCACAAGTCAA+TGG 0.553808 8_3:+19897898 Msa1280910:CDS
GACATGGCTGGTGTTGTGAT+AGG 0.556375 8_3:+19897018 Msa1280910:CDS
AGTTGGTTATGCAGAGGACA+TGG 0.556609 8_3:-19896404 None:intergenic
ATTTGTGGTAGCAAAGAAAG+AGG 0.560011 8_3:+19897606 Msa1280910:CDS
ACACCAGCCATGTCACATCC+AGG 0.560683 8_3:-19897009 None:intergenic
TTTGTGGTTGGTGGAGCAGG+TGG 0.565693 8_3:+19897285 Msa1280910:CDS
GTTCCTGGATGTGACATGGC+TGG 0.567085 8_3:+19897006 Msa1280910:CDS
ATTGAAACTGGAAGAGCTTG+GGG 0.577051 8_3:+19897808 Msa1280910:CDS
GCTGGTGTTGTGATAGGAAA+AGG 0.582500 8_3:+19897024 Msa1280910:CDS
GCAGAAAGCTTGGTTCTATG+AGG 0.584465 8_3:+19896337 Msa1280910:CDS
GCACTCACAGGAAACTCAGA+TGG 0.585262 8_3:-19896494 None:intergenic
TTTGCAAAACCATTGACTTG+TGG 0.585776 8_3:-19897907 None:intergenic
GGTGAGACAATGTTTGTGGT+TGG 0.593803 8_3:+19897273 Msa1280910:CDS
GCAGTTTGGTGCTGATAAAG+TGG 0.596691 8_3:+19897401 Msa1280910:CDS
TGAATCCTATCGATTCAAAG+AGG 0.603465 8_3:+19896453 Msa1280910:CDS
ATACGCCTCTTTGAATCGAT+AGG 0.608807 8_3:-19896458 None:intergenic
GAGCAATAGTTGACATAACA+TGG 0.608940 8_3:+19897629 Msa1280910:CDS
ATTGCTGTCTGAACTGCCAA+AGG 0.610038 8_3:-19897219 None:intergenic
GGTGGTTCCTGGATGTGACA+TGG 0.619307 8_3:+19897002 Msa1280910:intron
TAGCATCTATTGTAAGCAAG+AGG 0.619398 8_3:+19896102 Msa1280910:five_prime_UTR
ATTAATATAGTAACTACTTG+AGG 0.628995 8_3:-19897939 None:intergenic
AAAACTATCATGTATGTGTG+AGG 0.639036 8_3:-19896207 None:intergenic
ACAAGTAGTTGGTTATGCAG+AGG 0.650558 8_3:-19896410 None:intergenic
GCTACATGAAGAGACAACAT+AGG 0.653491 8_3:-19897355 None:intergenic
TTTGTGGTAGCAAAGAAAGA+GGG 0.664217 8_3:+19897607 Msa1280910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTAATTAGTAATTAAAAA+TGG - chr8_3:19897514-19897533 None:intergenic 10.0%
!! ATGTAATTAGTAATTAAAAA+TGG - chr8_3:19897514-19897533 None:intergenic 10.0%
!!! ATGGATAAACATTTTTTTAA+AGG + chr8_3:19896623-19896642 Msa1280910:intron 15.0%
!!! ATGATCTTATAAAAATTTTC+AGG + chr8_3:19897568-19897587 Msa1280910:intron 15.0%
!!! GTGTAATTTAAGTTATTATA+TGG + chr8_3:19898195-19898214 Msa1280910:three_prime_UTR 15.0%
!!! ATGGATAAACATTTTTTTAA+AGG + chr8_3:19896623-19896642 Msa1280910:intron 15.0%
!!! ATGATCTTATAAAAATTTTC+AGG + chr8_3:19897568-19897587 Msa1280910:intron 15.0%
!!! GTGTAATTTAAGTTATTATA+TGG + chr8_3:19898195-19898214 Msa1280910:three_prime_UTR 15.0%
!! TCAACTTACTATATATATCT+TGG + chr8_3:19896245-19896264 Msa1280910:five_prime_UTR 20.0%
!!! ATACATTGATTTTTATATGC+AGG + chr8_3:19896981-19897000 Msa1280910:intron 20.0%
!! AATGTCAAAAAATTTGACAT+AGG + chr8_3:19897051-19897070 Msa1280910:CDS 20.0%
!! ATTAATATAGTAACTACTTG+AGG - chr8_3:19897942-19897961 None:intergenic 20.0%
!!! TGTTGGTTTTTTTTTTTTGA+AGG + chr8_3:19897983-19898002 Msa1280910:intron 20.0%
!!! TTTTGAAGGAATTTTAATGT+TGG + chr8_3:19897997-19898016 Msa1280910:intron 20.0%
!! AAGTAATGTAATTTACCTTA+AGG + chr8_3:19898065-19898084 Msa1280910:three_prime_UTR 20.0%
!! TCAACTTACTATATATATCT+TGG + chr8_3:19896245-19896264 Msa1280910:five_prime_UTR 20.0%
!!! ATACATTGATTTTTATATGC+AGG + chr8_3:19896981-19897000 Msa1280910:intron 20.0%
!! AATGTCAAAAAATTTGACAT+AGG + chr8_3:19897051-19897070 Msa1280910:CDS 20.0%
!! ATTAATATAGTAACTACTTG+AGG - chr8_3:19897942-19897961 None:intergenic 20.0%
!!! TGTTGGTTTTTTTTTTTTGA+AGG + chr8_3:19897983-19898002 Msa1280910:intron 20.0%
!!! TTTTGAAGGAATTTTAATGT+TGG + chr8_3:19897997-19898016 Msa1280910:intron 20.0%
!! AAGTAATGTAATTTACCTTA+AGG + chr8_3:19898065-19898084 Msa1280910:three_prime_UTR 20.0%
!!! CTATCACATGATAATGTTTT+TGG + chr8_3:19896169-19896188 Msa1280910:five_prime_UTR 25.0%
!!! TAGATCTTTTTTCTCTCAAA+TGG - chr8_3:19896299-19896318 None:intergenic 25.0%
!!! AACATTTTTTTAAAGGACCA+TGG + chr8_3:19896630-19896649 Msa1280910:intron 25.0%
!! TTAATGGATATGTGAAGTTA+TGG + chr8_3:19896693-19896712 Msa1280910:intron 25.0%
!!! AGAGAATTCTTTTCTGAAAA+AGG - chr8_3:19896865-19896884 None:intergenic 25.0%
!!! AAAACAGGAGATTTTAAGAA+GGG + chr8_3:19897252-19897271 Msa1280910:CDS 25.0%
! GACTAAATATGAAGAAATTG+AGG + chr8_3:19897437-19897456 Msa1280910:CDS 25.0%
! AACTTTCTTTATGATACAGT+TGG + chr8_3:19897468-19897487 Msa1280910:CDS 25.0%
!! AGTAATTAAAAATGGATTCG+AGG - chr8_3:19897506-19897525 None:intergenic 25.0%
! TCGCATGTATTATAAAAAGT+TGG - chr8_3:19897539-19897558 None:intergenic 25.0%
! TTTATAATACATGCGAAAAG+TGG + chr8_3:19897543-19897562 Msa1280910:intron 25.0%
!! TCAAAATCATATTCACCTTT+TGG - chr8_3:19897763-19897782 None:intergenic 25.0%
! TTACTATATTAATCACTAGC+TGG + chr8_3:19897949-19897968 Msa1280910:three_prime_UTR 25.0%
!!! GAATTTTAATGTTGGTTATG+AGG + chr8_3:19898005-19898024 Msa1280910:intron 25.0%
!! TACATTATTTTCACACCTTA+AGG - chr8_3:19898083-19898102 None:intergenic 25.0%
!!! CTATCACATGATAATGTTTT+TGG + chr8_3:19896169-19896188 Msa1280910:five_prime_UTR 25.0%
!!! TAGATCTTTTTTCTCTCAAA+TGG - chr8_3:19896299-19896318 None:intergenic 25.0%
!!! AACATTTTTTTAAAGGACCA+TGG + chr8_3:19896630-19896649 Msa1280910:intron 25.0%
!! TTAATGGATATGTGAAGTTA+TGG + chr8_3:19896693-19896712 Msa1280910:intron 25.0%
!!! AGAGAATTCTTTTCTGAAAA+AGG - chr8_3:19896865-19896884 None:intergenic 25.0%
!!! AAAACAGGAGATTTTAAGAA+GGG + chr8_3:19897252-19897271 Msa1280910:CDS 25.0%
! GACTAAATATGAAGAAATTG+AGG + chr8_3:19897437-19897456 Msa1280910:CDS 25.0%
! AACTTTCTTTATGATACAGT+TGG + chr8_3:19897468-19897487 Msa1280910:CDS 25.0%
!! AGTAATTAAAAATGGATTCG+AGG - chr8_3:19897506-19897525 None:intergenic 25.0%
! TCGCATGTATTATAAAAAGT+TGG - chr8_3:19897539-19897558 None:intergenic 25.0%
! TTTATAATACATGCGAAAAG+TGG + chr8_3:19897543-19897562 Msa1280910:intron 25.0%
!! TCAAAATCATATTCACCTTT+TGG - chr8_3:19897763-19897782 None:intergenic 25.0%
! TTACTATATTAATCACTAGC+TGG + chr8_3:19897949-19897968 Msa1280910:three_prime_UTR 25.0%
!!! GAATTTTAATGTTGGTTATG+AGG + chr8_3:19898005-19898024 Msa1280910:intron 25.0%
!! TACATTATTTTCACACCTTA+AGG - chr8_3:19898083-19898102 None:intergenic 25.0%
GAGGATAGTATAACAGTTAT+AGG + chr8_3:19896136-19896155 Msa1280910:five_prime_UTR 30.0%
AAAACTATCATGTATGTGTG+AGG - chr8_3:19896210-19896229 None:intergenic 30.0%
! TCACACATACATGATAGTTT+TGG + chr8_3:19896209-19896228 Msa1280910:five_prime_UTR 30.0%
! CACACATACATGATAGTTTT+GGG + chr8_3:19896210-19896229 Msa1280910:five_prime_UTR 30.0%
ATATGAAAATGCAGAAAGCT+TGG + chr8_3:19896327-19896346 Msa1280910:exon 30.0%
! TCCGTTTTATAAGTTTCGAA+AGG + chr8_3:19896596-19896615 Msa1280910:intron 30.0%
CCCTTTCGAAACTTATAAAA+CGG - chr8_3:19896600-19896619 None:intergenic 30.0%
! CCGTTTTATAAGTTTCGAAA+GGG + chr8_3:19896597-19896616 Msa1280910:intron 30.0%
ATAAGTTTCGAAAGGGATAA+TGG + chr8_3:19896604-19896623 Msa1280910:intron 30.0%
TATAATATGCACTAGTTCCA+TGG - chr8_3:19896650-19896669 None:intergenic 30.0%
ATGGAACTAGTGCATATTAT+AGG + chr8_3:19896649-19896668 Msa1280910:intron 30.0%
GTATACTACAAGAATGAGTT+AGG + chr8_3:19896792-19896811 Msa1280910:intron 30.0%
!!! CATTGATTTTTATATGCAGG+TGG + chr8_3:19896984-19897003 Msa1280910:intron 30.0%
CATACAAGATTTCAACAGTA+TGG + chr8_3:19897092-19897111 Msa1280910:CDS 30.0%
ACCAAAATGTCTTTCATTTG+AGG + chr8_3:19897182-19897201 Msa1280910:CDS 30.0%
! TCCTCAAATGAAAGACATTT+TGG - chr8_3:19897186-19897205 None:intergenic 30.0%
!! CAAAACAGGAGATTTTAAGA+AGG + chr8_3:19897251-19897270 Msa1280910:CDS 30.0%
ACATAAGTTTAGCCAATTGA+AGG - chr8_3:19897326-19897345 None:intergenic 30.0%
CAATTGGCTAAACTTATGTT+TGG + chr8_3:19897327-19897346 Msa1280910:CDS 30.0%
! TGCTTCACAAATTTCACTTT+TGG - chr8_3:19897384-19897403 None:intergenic 30.0%
TGATTGCAAGAAATCATTTG+TGG + chr8_3:19897591-19897610 Msa1280910:CDS 30.0%
AAACTTGAATAAACTGCTCT+TGG - chr8_3:19897667-19897686 None:intergenic 30.0%
! TATTCAAGTTTGACAGTTAG+TGG + chr8_3:19897676-19897695 Msa1280910:CDS 30.0%
GAAAATGTGATTGATGCATT+TGG + chr8_3:19897781-19897800 Msa1280910:CDS 30.0%
GCATTTGGTTATATTGAAAC+TGG + chr8_3:19897796-19897815 Msa1280910:CDS 30.0%
GGTATCAATCTCAGATAAAA+TGG - chr8_3:19897881-19897900 None:intergenic 30.0%
ACTTATATTCCTAATGACTC+AGG + chr8_3:19898237-19898256 Msa1280910:three_prime_UTR 30.0%
CTTATATTCCTAATGACTCA+GGG + chr8_3:19898238-19898257 Msa1280910:three_prime_UTR 30.0%
GAGGATAGTATAACAGTTAT+AGG + chr8_3:19896136-19896155 Msa1280910:five_prime_UTR 30.0%
AAAACTATCATGTATGTGTG+AGG - chr8_3:19896210-19896229 None:intergenic 30.0%
! TCACACATACATGATAGTTT+TGG + chr8_3:19896209-19896228 Msa1280910:five_prime_UTR 30.0%
! CACACATACATGATAGTTTT+GGG + chr8_3:19896210-19896229 Msa1280910:five_prime_UTR 30.0%
ATATGAAAATGCAGAAAGCT+TGG + chr8_3:19896327-19896346 Msa1280910:exon 30.0%
! TCCGTTTTATAAGTTTCGAA+AGG + chr8_3:19896596-19896615 Msa1280910:intron 30.0%
CCCTTTCGAAACTTATAAAA+CGG - chr8_3:19896600-19896619 None:intergenic 30.0%
! CCGTTTTATAAGTTTCGAAA+GGG + chr8_3:19896597-19896616 Msa1280910:intron 30.0%
ATAAGTTTCGAAAGGGATAA+TGG + chr8_3:19896604-19896623 Msa1280910:intron 30.0%
TATAATATGCACTAGTTCCA+TGG - chr8_3:19896650-19896669 None:intergenic 30.0%
ATGGAACTAGTGCATATTAT+AGG + chr8_3:19896649-19896668 Msa1280910:intron 30.0%
GTATACTACAAGAATGAGTT+AGG + chr8_3:19896792-19896811 Msa1280910:intron 30.0%
!!! CATTGATTTTTATATGCAGG+TGG + chr8_3:19896984-19897003 Msa1280910:intron 30.0%
CATACAAGATTTCAACAGTA+TGG + chr8_3:19897092-19897111 Msa1280910:CDS 30.0%
ACCAAAATGTCTTTCATTTG+AGG + chr8_3:19897182-19897201 Msa1280910:CDS 30.0%
! TCCTCAAATGAAAGACATTT+TGG - chr8_3:19897186-19897205 None:intergenic 30.0%
!! CAAAACAGGAGATTTTAAGA+AGG + chr8_3:19897251-19897270 Msa1280910:CDS 30.0%
ACATAAGTTTAGCCAATTGA+AGG - chr8_3:19897326-19897345 None:intergenic 30.0%
CAATTGGCTAAACTTATGTT+TGG + chr8_3:19897327-19897346 Msa1280910:CDS 30.0%
! TGCTTCACAAATTTCACTTT+TGG - chr8_3:19897384-19897403 None:intergenic 30.0%
TGATTGCAAGAAATCATTTG+TGG + chr8_3:19897591-19897610 Msa1280910:CDS 30.0%
AAACTTGAATAAACTGCTCT+TGG - chr8_3:19897667-19897686 None:intergenic 30.0%
! TATTCAAGTTTGACAGTTAG+TGG + chr8_3:19897676-19897695 Msa1280910:CDS 30.0%
GAAAATGTGATTGATGCATT+TGG + chr8_3:19897781-19897800 Msa1280910:CDS 30.0%
GCATTTGGTTATATTGAAAC+TGG + chr8_3:19897796-19897815 Msa1280910:CDS 30.0%
GGTATCAATCTCAGATAAAA+TGG - chr8_3:19897881-19897900 None:intergenic 30.0%
TAGCATCTATTGTAAGCAAG+AGG + chr8_3:19896102-19896121 Msa1280910:five_prime_UTR 35.0%
!! TGAATCCTATCGATTCAAAG+AGG + chr8_3:19896453-19896472 Msa1280910:CDS 35.0%
GGAAACTCAGATGGAAAAAT+AGG - chr8_3:19896488-19896507 None:intergenic 35.0%
GTCAATCATGTGACAGTTAA+TGG + chr8_3:19896677-19896696 Msa1280910:intron 35.0%
GCAATTGAAGGATTCAAAAC+AGG + chr8_3:19897237-19897256 Msa1280910:CDS 35.0%
GTGAAATTTGTGAAGCAGTT+TGG + chr8_3:19897387-19897406 Msa1280910:CDS 35.0%
ATTTGTGGTAGCAAAGAAAG+AGG + chr8_3:19897606-19897625 Msa1280910:CDS 35.0%
TTTGTGGTAGCAAAGAAAGA+GGG + chr8_3:19897607-19897626 Msa1280910:CDS 35.0%
GAGCAATAGTTGACATAACA+TGG + chr8_3:19897629-19897648 Msa1280910:CDS 35.0%
GACAGTTAGTGGTGAAATAT+TGG + chr8_3:19897687-19897706 Msa1280910:CDS 35.0%
AACTCTCCTCTTTCCAAATA+TGG - chr8_3:19897724-19897743 None:intergenic 35.0%
AAAGCAGTGATTGATCCAAA+AGG + chr8_3:19897745-19897764 Msa1280910:CDS 35.0%
ATATTGAAACTGGAAGAGCT+TGG + chr8_3:19897806-19897825 Msa1280910:CDS 35.0%
TATTGAAACTGGAAGAGCTT+GGG + chr8_3:19897807-19897826 Msa1280910:CDS 35.0%
! AGTGATGATGATGAATAGGA+GGG - chr8_3:19897855-19897874 None:intergenic 35.0%
! GTAGAGTGATGATGATGAAT+AGG - chr8_3:19897859-19897878 None:intergenic 35.0%
ACCAATATACCACAAGTCAA+TGG + chr8_3:19897898-19897917 Msa1280910:CDS 35.0%
!! ACCATTGACTTGTGGTATAT+TGG - chr8_3:19897902-19897921 None:intergenic 35.0%
!! TTTGCAAAACCATTGACTTG+TGG - chr8_3:19897910-19897929 None:intergenic 35.0%
AGCTGGTGACAATTTAATGT+TGG + chr8_3:19897966-19897985 Msa1280910:three_prime_UTR 35.0%
TAGCATCTATTGTAAGCAAG+AGG + chr8_3:19896102-19896121 Msa1280910:five_prime_UTR 35.0%
!! TGAATCCTATCGATTCAAAG+AGG + chr8_3:19896453-19896472 Msa1280910:CDS 35.0%
GGAAACTCAGATGGAAAAAT+AGG - chr8_3:19896488-19896507 None:intergenic 35.0%
GTCAATCATGTGACAGTTAA+TGG + chr8_3:19896677-19896696 Msa1280910:intron 35.0%
GCAATTGAAGGATTCAAAAC+AGG + chr8_3:19897237-19897256 Msa1280910:CDS 35.0%
GTGAAATTTGTGAAGCAGTT+TGG + chr8_3:19897387-19897406 Msa1280910:CDS 35.0%
ATTTGTGGTAGCAAAGAAAG+AGG + chr8_3:19897606-19897625 Msa1280910:CDS 35.0%
TTTGTGGTAGCAAAGAAAGA+GGG + chr8_3:19897607-19897626 Msa1280910:CDS 35.0%
GAGCAATAGTTGACATAACA+TGG + chr8_3:19897629-19897648 Msa1280910:CDS 35.0%
GACAGTTAGTGGTGAAATAT+TGG + chr8_3:19897687-19897706 Msa1280910:CDS 35.0%
AACTCTCCTCTTTCCAAATA+TGG - chr8_3:19897724-19897743 None:intergenic 35.0%
AAAGCAGTGATTGATCCAAA+AGG + chr8_3:19897745-19897764 Msa1280910:CDS 35.0%
ATATTGAAACTGGAAGAGCT+TGG + chr8_3:19897806-19897825 Msa1280910:CDS 35.0%
TATTGAAACTGGAAGAGCTT+GGG + chr8_3:19897807-19897826 Msa1280910:CDS 35.0%
! AGTGATGATGATGAATAGGA+GGG - chr8_3:19897855-19897874 None:intergenic 35.0%
! GTAGAGTGATGATGATGAAT+AGG - chr8_3:19897859-19897878 None:intergenic 35.0%
ACCAATATACCACAAGTCAA+TGG + chr8_3:19897898-19897917 Msa1280910:CDS 35.0%
!! ACCATTGACTTGTGGTATAT+TGG - chr8_3:19897902-19897921 None:intergenic 35.0%
!! TTTGCAAAACCATTGACTTG+TGG - chr8_3:19897910-19897929 None:intergenic 35.0%
AGCTGGTGACAATTTAATGT+TGG + chr8_3:19897966-19897985 Msa1280910:three_prime_UTR 35.0%
GCAAGAGGATAGAACATAAG+AGG + chr8_3:19896117-19896136 Msa1280910:five_prime_UTR 40.0%
TAGGAAAGTCTCCTAGCTTA+AGG - chr8_3:19896382-19896401 None:intergenic 40.0%
TTATGCAGAGGACATGGAAT+AGG - chr8_3:19896401-19896420 None:intergenic 40.0%
ACAAGTAGTTGGTTATGCAG+AGG - chr8_3:19896413-19896432 None:intergenic 40.0%
CATGAACTTGGACAAGTAGT+TGG - chr8_3:19896424-19896443 None:intergenic 40.0%
ATACGCCTCTTTGAATCGAT+AGG - chr8_3:19896461-19896480 None:intergenic 40.0%
TTAGAGTTTCAAGCACTCAC+AGG - chr8_3:19896509-19896528 None:intergenic 40.0%
!! AAGCAACTTGCTAGGAATCT+TGG - chr8_3:19896829-19896848 None:intergenic 40.0%
! TATGCAACAAGCAACTTGCT+AGG - chr8_3:19896837-19896856 None:intergenic 40.0%
! AGACACACAATCAGAAGTGT+CGG + chr8_3:19896922-19896941 Msa1280910:intron 40.0%
! TTTTATATGCAGGTGGTTCC+TGG + chr8_3:19896991-19897010 Msa1280910:intron 40.0%
GACATAGGTGATGAAGTCTA+TGG + chr8_3:19897066-19897085 Msa1280910:CDS 40.0%
ATGGAAAAACCAAAGCAGCT+TGG + chr8_3:19897111-19897130 Msa1280910:CDS 40.0%
TGGAACTTTGGTCCTTCAAT+TGG + chr8_3:19897311-19897330 Msa1280910:CDS 40.0%
GCTACATGAAGAGACAACAT+AGG - chr8_3:19897358-19897377 None:intergenic 40.0%
GGAAAAGCTGAGACCATATT+TGG + chr8_3:19897708-19897727 Msa1280910:CDS 40.0%
CTGAGACCATATTTGGAAAG+AGG + chr8_3:19897715-19897734 Msa1280910:CDS 40.0%
ATTGAAACTGGAAGAGCTTG+GGG + chr8_3:19897808-19897827 Msa1280910:CDS 40.0%
! GAGTGATGATGATGAATAGG+AGG - chr8_3:19897856-19897875 None:intergenic 40.0%
GCAAGAGGATAGAACATAAG+AGG + chr8_3:19896117-19896136 Msa1280910:five_prime_UTR 40.0%
TAGGAAAGTCTCCTAGCTTA+AGG - chr8_3:19896382-19896401 None:intergenic 40.0%
TTATGCAGAGGACATGGAAT+AGG - chr8_3:19896401-19896420 None:intergenic 40.0%
ACAAGTAGTTGGTTATGCAG+AGG - chr8_3:19896413-19896432 None:intergenic 40.0%
CATGAACTTGGACAAGTAGT+TGG - chr8_3:19896424-19896443 None:intergenic 40.0%
ATACGCCTCTTTGAATCGAT+AGG - chr8_3:19896461-19896480 None:intergenic 40.0%
TTAGAGTTTCAAGCACTCAC+AGG - chr8_3:19896509-19896528 None:intergenic 40.0%
!! AAGCAACTTGCTAGGAATCT+TGG - chr8_3:19896829-19896848 None:intergenic 40.0%
! TATGCAACAAGCAACTTGCT+AGG - chr8_3:19896837-19896856 None:intergenic 40.0%
! AGACACACAATCAGAAGTGT+CGG + chr8_3:19896922-19896941 Msa1280910:intron 40.0%
! TTTTATATGCAGGTGGTTCC+TGG + chr8_3:19896991-19897010 Msa1280910:intron 40.0%
GACATAGGTGATGAAGTCTA+TGG + chr8_3:19897066-19897085 Msa1280910:CDS 40.0%
ATGGAAAAACCAAAGCAGCT+TGG + chr8_3:19897111-19897130 Msa1280910:CDS 40.0%
TGGAACTTTGGTCCTTCAAT+TGG + chr8_3:19897311-19897330 Msa1280910:CDS 40.0%
GCTACATGAAGAGACAACAT+AGG - chr8_3:19897358-19897377 None:intergenic 40.0%
GGAAAAGCTGAGACCATATT+TGG + chr8_3:19897708-19897727 Msa1280910:CDS 40.0%
CTGAGACCATATTTGGAAAG+AGG + chr8_3:19897715-19897734 Msa1280910:CDS 40.0%
ATTGAAACTGGAAGAGCTTG+GGG + chr8_3:19897808-19897827 Msa1280910:CDS 40.0%
! GAGTGATGATGATGAATAGG+AGG - chr8_3:19897856-19897875 None:intergenic 40.0%
!! GCAGAAAGCTTGGTTCTATG+AGG + chr8_3:19896337-19896356 Msa1280910:CDS 45.0%
!! GCTTGGTTCTATGAGGAGTA+TGG + chr8_3:19896344-19896363 Msa1280910:CDS 45.0%
! CTATGAGGAGTATGGTCCTA+AGG + chr8_3:19896352-19896371 Msa1280910:CDS 45.0%
CCTAGCTTAAGGACTTCCTT+AGG - chr8_3:19896371-19896390 None:intergenic 45.0%
CCTAAGGAAGTCCTTAAGCT+AGG + chr8_3:19896368-19896387 Msa1280910:CDS 45.0%
AGTTGGTTATGCAGAGGACA+TGG - chr8_3:19896407-19896426 None:intergenic 45.0%
TCAAAGCAGCAGCATGAACT+TGG - chr8_3:19896436-19896455 None:intergenic 45.0%
GCATACGAAAGTTACCACTG+TGG - chr8_3:19896950-19896969 None:intergenic 45.0%
!! GCTGGTGTTGTGATAGGAAA+AGG + chr8_3:19897024-19897043 Msa1280910:CDS 45.0%
ACCAAAGCAGCTTGGAACAT+TGG + chr8_3:19897119-19897138 Msa1280910:CDS 45.0%
GGAAGCTGCAAGTTTACCTT+TGG + chr8_3:19897203-19897222 Msa1280910:CDS 45.0%
ATTGCTGTCTGAACTGCCAA+AGG - chr8_3:19897222-19897241 None:intergenic 45.0%
GCAGTTCAGACAGCAATTGA+AGG + chr8_3:19897225-19897244 Msa1280910:CDS 45.0%
GAAGGGTGAGACAATGTTTG+TGG + chr8_3:19897269-19897288 Msa1280910:CDS 45.0%
GGTGAGACAATGTTTGTGGT+TGG + chr8_3:19897273-19897292 Msa1280910:CDS 45.0%
GAGACAATGTTTGTGGTTGG+TGG + chr8_3:19897276-19897295 Msa1280910:CDS 45.0%
!! GCAGTTTGGTGCTGATAAAG+TGG + chr8_3:19897401-19897420 Msa1280910:CDS 45.0%
!! GCAGAAAGCTTGGTTCTATG+AGG + chr8_3:19896337-19896356 Msa1280910:CDS 45.0%
!! GCTTGGTTCTATGAGGAGTA+TGG + chr8_3:19896344-19896363 Msa1280910:CDS 45.0%
! CTATGAGGAGTATGGTCCTA+AGG + chr8_3:19896352-19896371 Msa1280910:CDS 45.0%
CCTAGCTTAAGGACTTCCTT+AGG - chr8_3:19896371-19896390 None:intergenic 45.0%
CCTAAGGAAGTCCTTAAGCT+AGG + chr8_3:19896368-19896387 Msa1280910:CDS 45.0%
AGTTGGTTATGCAGAGGACA+TGG - chr8_3:19896407-19896426 None:intergenic 45.0%
TCAAAGCAGCAGCATGAACT+TGG - chr8_3:19896436-19896455 None:intergenic 45.0%
GCATACGAAAGTTACCACTG+TGG - chr8_3:19896950-19896969 None:intergenic 45.0%
!! GCTGGTGTTGTGATAGGAAA+AGG + chr8_3:19897024-19897043 Msa1280910:CDS 45.0%
ACCAAAGCAGCTTGGAACAT+TGG + chr8_3:19897119-19897138 Msa1280910:CDS 45.0%
GGAAGCTGCAAGTTTACCTT+TGG + chr8_3:19897203-19897222 Msa1280910:CDS 45.0%
ATTGCTGTCTGAACTGCCAA+AGG - chr8_3:19897222-19897241 None:intergenic 45.0%
GCAGTTCAGACAGCAATTGA+AGG + chr8_3:19897225-19897244 Msa1280910:CDS 45.0%
GAAGGGTGAGACAATGTTTG+TGG + chr8_3:19897269-19897288 Msa1280910:CDS 45.0%
GGTGAGACAATGTTTGTGGT+TGG + chr8_3:19897273-19897292 Msa1280910:CDS 45.0%
GAGACAATGTTTGTGGTTGG+TGG + chr8_3:19897276-19897295 Msa1280910:CDS 45.0%
!! GCAGTTTGGTGCTGATAAAG+TGG + chr8_3:19897401-19897420 Msa1280910:CDS 45.0%
GCACTCACAGGAAACTCAGA+TGG - chr8_3:19896497-19896516 None:intergenic 50.0%
!! GACATGGCTGGTGTTGTGAT+AGG + chr8_3:19897018-19897037 Msa1280910:CDS 50.0%
GCCAATGTTCCAAGCTGCTT+TGG - chr8_3:19897123-19897142 None:intergenic 50.0%
!! ATGTTTGTGGTTGGTGGAGC+AGG + chr8_3:19897282-19897301 Msa1280910:CDS 50.0%
! AGCAGGTGGTGTTGGAACTT+TGG + chr8_3:19897299-19897318 Msa1280910:CDS 50.0%
ACTGCTCTTGGATGTGATGC+TGG - chr8_3:19897655-19897674 None:intergenic 50.0%
GCACTCACAGGAAACTCAGA+TGG - chr8_3:19896497-19896516 None:intergenic 50.0%
!! GACATGGCTGGTGTTGTGAT+AGG + chr8_3:19897018-19897037 Msa1280910:CDS 50.0%
GCCAATGTTCCAAGCTGCTT+TGG - chr8_3:19897123-19897142 None:intergenic 50.0%
!! ATGTTTGTGGTTGGTGGAGC+AGG + chr8_3:19897282-19897301 Msa1280910:CDS 50.0%
! AGCAGGTGGTGTTGGAACTT+TGG + chr8_3:19897299-19897318 Msa1280910:CDS 50.0%
ACTGCTCTTGGATGTGATGC+TGG - chr8_3:19897655-19897674 None:intergenic 50.0%
GGTGGTTCCTGGATGTGACA+TGG + chr8_3:19897002-19897021 Msa1280910:intron 55.0%
GTTCCTGGATGTGACATGGC+TGG + chr8_3:19897006-19897025 Msa1280910:CDS 55.0%
ACACCAGCCATGTCACATCC+AGG - chr8_3:19897012-19897031 None:intergenic 55.0%
!! TTTGTGGTTGGTGGAGCAGG+TGG + chr8_3:19897285-19897304 Msa1280910:CDS 55.0%
GGTGGTTCCTGGATGTGACA+TGG + chr8_3:19897002-19897021 Msa1280910:intron 55.0%
GTTCCTGGATGTGACATGGC+TGG + chr8_3:19897006-19897025 Msa1280910:CDS 55.0%
ACACCAGCCATGTCACATCC+AGG - chr8_3:19897012-19897031 None:intergenic 55.0%
!! TTTGTGGTTGGTGGAGCAGG+TGG + chr8_3:19897285-19897304 Msa1280910:CDS 55.0%
!! CAGAAGTGTCGGTGCCACAG+TGG + chr8_3:19896933-19896952 Msa1280910:intron 60.0%
! GTTGGTGGAGCAGGTGGTGT+TGG + chr8_3:19897291-19897310 Msa1280910:CDS 60.0%
!! CAGAAGTGTCGGTGCCACAG+TGG + chr8_3:19896933-19896952 Msa1280910:intron 60.0%
! GTTGGTGGAGCAGGTGGTGT+TGG + chr8_3:19897291-19897310 Msa1280910:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 19896098 19898257 19896098 ID=Msa1280910;Name=Msa1280910
chr8_3 mRNA 19896098 19898257 19896098 ID=Msa1280910-mRNA-1;Parent=Msa1280910;Name=Msa1280910-mRNA-1;_AED=0.02;_eAED=0.02;_QI=231|1|1|1|0.5|0.4|5|236|341
chr8_3 exon 19896098 19896508 19896098 ID=Msa1280910-mRNA-1:exon:7353;Parent=Msa1280910-mRNA-1
chr8_3 exon 19897003 19897489 19897003 ID=Msa1280910-mRNA-1:exon:7354;Parent=Msa1280910-mRNA-1
chr8_3 exon 19897590 19897987 19897590 ID=Msa1280910-mRNA-1:exon:7355;Parent=Msa1280910-mRNA-1
chr8_3 exon 19898027 19898147 19898027 ID=Msa1280910-mRNA-1:exon:7356;Parent=Msa1280910-mRNA-1
chr8_3 exon 19898182 19898257 19898182 ID=Msa1280910-mRNA-1:exon:7357;Parent=Msa1280910-mRNA-1
chr8_3 five_prime_UTR 19896098 19896328 19896098 ID=Msa1280910-mRNA-1:five_prime_utr;Parent=Msa1280910-mRNA-1
chr8_3 CDS 19896329 19896508 19896329 ID=Msa1280910-mRNA-1:cds;Parent=Msa1280910-mRNA-1
chr8_3 CDS 19897003 19897489 19897003 ID=Msa1280910-mRNA-1:cds;Parent=Msa1280910-mRNA-1
chr8_3 CDS 19897590 19897948 19897590 ID=Msa1280910-mRNA-1:cds;Parent=Msa1280910-mRNA-1
chr8_3 three_prime_UTR 19897949 19897987 19897949 ID=Msa1280910-mRNA-1:three_prime_utr;Parent=Msa1280910-mRNA-1
chr8_3 three_prime_UTR 19898027 19898147 19898027 ID=Msa1280910-mRNA-1:three_prime_utr;Parent=Msa1280910-mRNA-1
chr8_3 three_prime_UTR 19898182 19898257 19898182 ID=Msa1280910-mRNA-1:three_prime_utr;Parent=Msa1280910-mRNA-1
Gene Sequence

>Msa1280910

ATGAAAATGCAGAAAGCTTGGTTCTATGAGGAGTATGGTCCTAAGGAAGTCCTTAAGCTAGGAGACTTTCCTATTCCATGTCCTCTGCATAACCAACTACTTGTCCAAGTTCATGCTGCTGCTTTGAATCCTATCGATTCAAAGAGGCGTATGCGTCCTATTTTTCCATCTGAGTTTCCTGTGGTTCCTGGATGTGACATGGCTGGTGTTGTGATAGGAAAAGGAATTAATGTCAAAAAATTTGACATAGGTGATGAAGTCTATGGAAACATACAAGATTTCAACAGTATGGAAAAACCAAAGCAGCTTGGAACATTGGCACAGTTCATTGTTGTAGAAGAGAACTTAGTTGCAAGAAAACCAAAATGTCTTTCATTTGAGGAAGCTGCAAGTTTACCTTTGGCAGTTCAGACAGCAATTGAAGGATTCAAAACAGGAGATTTTAAGAAGGGTGAGACAATGTTTGTGGTTGGTGGAGCAGGTGGTGTTGGAACTTTGGTCCTTCAATTGGCTAAACTTATGTTTGGAGCTTCCTATGTTGTCTCTTCATGTAGCACTCCAAAAGTGAAATTTGTGAAGCAGTTTGGTGCTGATAAAGTGGTTGACTATACTAAGACTAAATATGAAGAAATTGAGGAGAAATTTAACTTTCTTTATGATACAGTTGGTGATTGCAAGAAATCATTTGTGGTAGCAAAGAAAGAGGGAGCAATAGTTGACATAACATGGCCAGCATCACATCCAAGAGCAGTTTATTCAAGTTTGACAGTTAGTGGTGAAATATTGGAAAAGCTGAGACCATATTTGGAAAGAGGAGAGTTGAAAGCAGTGATTGATCCAAAAGGTGAATATGATTTTGAAAATGTGATTGATGCATTTGGTTATATTGAAACTGGAAGAGCTTGGGGTAAAGTTGTTGTCACATGCTTCCCTCCTATTCATCATCATCACTCTACCATTTTATCTGAGATTGATACCAATATACCACAAGTCAATGGTTTTGCAAAAGATCTTTGCCTCAAGTAG
Protein sequence

>Msa1280910

MKMQKAWFYEEYGPKEVLKLGDFPIPCPLHNQLLVQVHAAALNPIDSKRRMRPIFPSEFPVVPGCDMAGVVIGKGINVKKFDIGDEVYGNIQDFNSMEKPKQLGTLAQFIVVEENLVARKPKCLSFEEAASLPLAVQTAIEGFKTGDFKKGETMFVVGGAGGVGTLVLQLAKLMFGASYVVSSCSTPKVKFVKQFGADKVVDYTKTKYEEIEEKFNFLYDTVGDCKKSFVVAKKEGAIVDITWPASHPRAVYSSLTVSGEILEKLRPYLERGELKAVIDPKGEYDFENVIDAFGYIETGRAWGKVVVTCFPPIHHHHSTILSEIDTNIPQVNGFAKDLCLK*