AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1186200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1186200 MtrunA17_Chr1g0203051 47.690 606 314 1 35 640 19 621 0.0 598
Msa1186200 MtrunA17_Chr2g0287651 35.027 748 452 8 47 775 12 744 1.81e-151 465
Msa1186200 MtrunA17_Chr2g0308841 75.221 226 56 0 427 652 1 226 2.03e-126 379
Msa1186200 MtrunA17_Chr5g0448761 31.070 589 392 7 103 678 65 652 2.08e-101 331
Msa1186200 MtrunA17_Chr1g0202341 31.272 566 373 7 103 660 56 613 3.74e-98 320
Msa1186200 MtrunA17_Chr8g0363841 30.526 570 377 7 106 663 25 587 1.43e-85 286
Msa1186200 MtrunA17_Chr1g0198761 29.760 793 479 24 32 790 1 749 1.75e-85 288
Msa1186200 MtrunA17_Chr2g0324011 29.590 561 376 9 104 653 16 568 3.71e-83 277
Msa1186200 MtrunA17_Chr5g0441581 28.966 580 369 13 104 662 38 595 6.92e-74 254
Msa1186200 MtrunA17_Chr5g0440451 27.856 639 406 12 70 678 16 629 1.83e-73 258
Msa1186200 MtrunA17_Chr2g0296781 27.803 651 434 18 113 744 12 645 3.14e-71 248
Msa1186200 MtrunA17_Chr6g0458391 27.402 562 369 10 104 653 231 765 5.95e-71 248
Msa1186200 MtrunA17_Chr6g0458391 35.294 119 66 4 103 217 96 207 2.33e-11 67.8
Msa1186200 MtrunA17_Chr1g0203721 28.799 566 376 12 104 653 69 623 1.54e-70 249
Msa1186200 MtrunA17_Chr2g0287061 30.245 572 322 14 103 654 188 702 5.23e-70 246
Msa1186200 MtrunA17_Chr1g0207551 28.690 603 398 16 70 658 8 592 1.05e-66 237
Msa1186200 MtrunA17_Chr5g0448511 29.211 380 263 2 279 653 19 397 3.75e-66 230
Msa1186200 MtrunA17_Chr3g0118611 29.594 419 287 3 265 678 111 526 4.78e-66 234
Msa1186200 MtrunA17_Chr6g0477261 28.835 541 354 14 224 744 5 534 2.49e-65 230
Msa1186200 MtrunA17_Chr5g0431001 33.095 420 254 8 104 502 67 480 9.10e-64 222
Msa1186200 MtrunA17_Chr2g0276841 28.846 572 365 15 104 652 76 628 3.21e-63 225
Msa1186200 MtrunA17_Chr2g0290391 27.247 712 446 16 68 752 25 691 3.97e-63 225
Msa1186200 MtrunA17_Chr1g0196541 26.889 569 382 12 104 653 3 556 8.74e-61 219
Msa1186200 MtrunA17_Chr3g0082121 26.773 564 379 10 103 653 48 590 3.97e-60 216
Msa1186200 MtrunA17_Chr3g0080351 27.700 574 361 14 103 653 81 623 4.79e-59 216
Msa1186200 MtrunA17_Chr2g0291591 27.723 505 340 11 103 595 69 560 3.77e-57 205
Msa1186200 MtrunA17_Chr2g0329031 25.718 696 454 20 91 754 7 671 7.94e-56 205
Msa1186200 MtrunA17_Chr2g0291581 24.409 635 439 18 103 720 76 686 3.41e-55 202
Msa1186200 MtrunA17_Chr2g0294841 28.546 564 373 12 104 648 51 603 8.99e-54 199
Msa1186200 MtrunA17_Chr1g0164591 27.451 306 206 4 187 485 11 307 4.26e-36 139
Msa1186200 MtrunA17_Chr7g0258861 28.028 289 198 5 104 386 16 300 2.77e-31 124
Msa1186200 MtrunA17_Chr1g0171741 33.607 244 133 4 6 221 13 255 3.28e-30 120
Msa1186200 MtrunA17_Chr8g0389811 37.736 159 93 2 52 209 42 195 5.61e-29 115
Msa1186200 MtrunA17_Chr2g0331371 38.690 168 101 1 42 209 1 166 1.37e-27 111
Msa1186200 MtrunA17_Chr8g0340801 39.048 105 64 0 209 313 1 105 1.38e-22 93.2
Msa1186200 MtrunA17_Chr6g0473421 45.055 91 50 0 83 173 40 130 6.02e-21 90.1
Msa1186200 MtrunA17_Chr1g0172931 42.609 115 59 3 70 180 47 158 4.60e-19 85.1
Msa1186200 MtrunA17_Chr2g0277171 60.241 83 29 2 1 83 1 79 1.36e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1186200 AT4G38180.1 32.218 568 370 9 98 653 70 634 1.41e-96 319
Msa1186200 AT3G06250.2 31.720 558 326 13 103 653 195 704 3.30e-80 274
Msa1186200 AT3G06250.1 31.720 558 326 13 103 653 195 704 3.30e-80 274
Msa1186200 AT3G06250.3 31.720 558 326 13 103 653 195 704 3.30e-80 274
Msa1186200 AT5G18960.2 30.935 556 336 14 103 653 216 728 6.48e-76 263
Msa1186200 AT5G18960.1 30.935 556 336 14 103 653 216 728 6.48e-76 263
Msa1186200 AT4G15090.1 26.923 598 395 11 82 653 28 609 1.36e-69 246
Msa1186200 AT4G15090.5 26.923 598 395 11 82 653 28 609 1.36e-69 246
Msa1186200 AT4G15090.3 26.923 598 395 11 82 653 28 609 1.36e-69 246
Msa1186200 AT4G15090.2 26.923 598 395 11 82 653 28 609 1.36e-69 246
Msa1186200 AT4G15090.4 26.923 598 395 11 82 653 28 609 1.36e-69 246
Msa1186200 AT2G27110.2 26.916 561 371 10 103 656 54 582 5.25e-68 242
Msa1186200 AT2G27110.1 26.916 561 371 10 103 656 54 582 5.25e-68 242
Msa1186200 AT1G10240.2 29.912 565 368 14 104 652 54 606 4.44e-65 230
Msa1186200 AT1G10240.1 29.912 565 368 14 104 652 54 606 4.44e-65 230
Msa1186200 AT2G27110.3 28.535 396 275 3 266 656 45 437 8.39e-65 230
Msa1186200 AT3G22170.2 26.387 595 380 14 84 653 57 618 2.16e-62 226
Msa1186200 AT3G22170.1 26.387 595 380 14 84 653 57 618 2.16e-62 226
Msa1186200 AT1G80010.1 29.082 588 378 15 103 665 100 673 8.65e-62 222
Msa1186200 AT1G76320.3 28.342 561 376 10 103 654 2 545 4.77e-61 219
Msa1186200 AT1G76320.2 28.342 561 376 10 103 654 2 545 6.30e-61 220
Msa1186200 AT1G52520.1 26.087 667 456 15 70 720 57 702 7.31e-61 219
Msa1186200 AT1G76320.1 28.342 561 376 10 103 654 2 545 7.96e-61 219
Msa1186200 AT1G76320.4 28.342 561 376 10 103 654 2 545 7.96e-61 219
Msa1186200 AT2G32250.5 25.766 555 358 9 103 650 43 550 1.47e-58 214
Msa1186200 AT2G32250.8 25.766 555 358 9 103 650 2 509 1.55e-58 213
Msa1186200 AT2G32250.7 25.766 555 358 9 103 650 2 509 1.91e-58 213
Msa1186200 AT2G32250.6 25.766 555 358 9 103 650 43 550 2.02e-58 214
Msa1186200 AT2G32250.1 25.766 555 358 9 103 650 43 550 2.02e-58 214
Msa1186200 AT2G32250.3 25.766 555 358 9 103 650 43 550 2.02e-58 214
Msa1186200 AT2G32250.2 25.766 555 358 9 103 650 43 550 2.13e-58 214
Msa1186200 AT2G32250.4 25.766 555 358 9 103 650 43 550 2.13e-58 214
Msa1186200 AT4G38170.1 27.621 391 270 6 270 653 15 399 1.35e-54 198
Msa1186200 AT5G28530.3 27.417 569 376 11 104 650 61 614 2.13e-54 200
Msa1186200 AT5G28530.2 27.417 569 376 11 104 650 61 614 2.13e-54 200
Msa1186200 AT5G28530.1 27.592 569 379 11 104 650 61 618 2.25e-54 200
Msa1186200 AT4G19990.5 25.429 641 414 15 98 716 22 620 3.02e-51 192
Msa1186200 AT4G19990.3 25.429 641 414 15 98 716 22 620 3.23e-51 192
Msa1186200 AT4G19990.4 25.429 641 414 15 98 716 22 620 3.23e-51 192
Msa1186200 AT4G19990.7 25.429 641 414 15 98 716 36 634 3.49e-51 192
Msa1186200 AT4G19990.6 25.429 641 414 15 98 716 22 620 3.91e-51 192
Msa1186200 AT4G19990.2 25.429 641 414 15 98 716 22 620 3.91e-51 192
Msa1186200 AT4G19990.1 24.917 602 390 13 136 716 3 563 3.11e-48 182
Msa1186200 AT5G28530.5 24.910 558 341 13 104 650 61 551 2.02e-35 143
Msa1186200 AT5G28530.4 25.090 558 344 13 104 650 61 555 2.15e-35 143

Find 181 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTCTGGGTGCATCTTTA+AGG 0.099129 8_1:-24268173 Msa1186200:CDS
TTCTTAAACAAGAACATTTA+AGG 0.185418 8_1:+24268089 None:intergenic
GAACACCAATATTTAAATTT+AGG 0.214051 8_1:+24267585 None:intergenic
TGCATGCGTCTGCGCCTGCC+TGG 0.226208 8_1:+24267659 None:intergenic
TGTTGGTTTGCTAGAATGTC+TGG 0.244371 8_1:-24269573 Msa1186200:CDS
CCACGCCTAAATTTAAATAT+TGG 0.252621 8_1:-24267590 Msa1186200:CDS
AGCAATTGTGTTCAACGATA+TGG 0.256143 8_1:-24269688 Msa1186200:CDS
CTATGCTAAGGAGTCATTTC+TGG 0.258823 8_1:-24267350 Msa1186200:three_prime_UTR
TCATTTACCGTCTGCAAAAC+TGG 0.293721 8_1:+24267749 None:intergenic
TTAATGTGTTTGAAAGGTTA+TGG 0.298423 8_1:-24268597 Msa1186200:CDS
CTCTTCAGATTCGGTAGTTT+CGG 0.299776 8_1:+24269755 None:intergenic
GTGGACAACATCGTTTATTA+TGG 0.307273 8_1:+24267609 None:intergenic
GAATGTCAAGAAATGTCTTC+TGG 0.313620 8_1:-24268187 Msa1186200:CDS
CGAATGAACTACGAGATATT+TGG 0.314403 8_1:-24268979 Msa1186200:CDS
TGATGAGCTTCCCAATTGTT+TGG 0.327105 8_1:-24268008 Msa1186200:CDS
TGATATAATGAAGTTTGATA+TGG 0.333774 8_1:-24269664 Msa1186200:CDS
CATTACGTGGCTCACGCTTT+CGG 0.334984 8_1:+24269436 None:intergenic
TGCAGCATTTGTGGTGTAAT+AGG 0.340181 8_1:-24267632 Msa1186200:CDS
CATAGTCGGTGATGCGCATT+GGG 0.340887 8_1:+24268712 None:intergenic
TTAAGATAAGACATAACTTC+TGG 0.343770 8_1:+24268646 None:intergenic
CTGTACTATACCGGAATATC+TGG 0.344012 8_1:+24267898 None:intergenic
GCTGAGATGGTTCAGAAGTT+TGG 0.352261 8_1:-24268574 Msa1186200:CDS
TACGTAAGGGTCAAATGATT+AGG 0.360442 8_1:-24269542 Msa1186200:CDS
AAAAGCTTGAGAGATTGTTT+TGG 0.360617 8_1:-24269017 Msa1186200:CDS
TTGCCTACATGTTCTATTGT+TGG 0.362832 8_1:-24269590 Msa1186200:CDS
CAATCTGCAGCCAGATATTC+CGG 0.371485 8_1:-24267908 Msa1186200:CDS
TGGTGTTCAGTCACTTTCTC+CGG 0.372522 8_1:-24267570 Msa1186200:CDS
TTGGTTTAGAAGACAACAAT+TGG 0.378277 8_1:-24268555 Msa1186200:CDS
TAAATGTTCTTGTTTAAGAA+TGG 0.382832 8_1:-24268086 Msa1186200:CDS
CCTAGGCAAAACCAAACAAT+TGG 0.383097 8_1:+24267997 None:intergenic
GTAATAGGATGCTTGTGATT+TGG 0.390458 8_1:-24267105 Msa1186200:three_prime_UTR
GCATGCGTCTGCGCCTGCCT+GGG 0.395995 8_1:+24267660 None:intergenic
ACAAAAGGTTGTGGTTCAAT+TGG 0.406384 8_1:-24267692 Msa1186200:CDS
GTGAAACCCGCCTGCTGCAT+TGG 0.415584 8_1:+24269191 None:intergenic
ATTGTAGTGGTTCTGTGTAC+TGG 0.418264 8_1:-24267937 Msa1186200:CDS
GTCATTTCTGGAATGTTTCA+AGG 0.422882 8_1:-24267338 Msa1186200:three_prime_UTR
TGTGGGCCACTGCTCACATC+AGG 0.426152 8_1:-24268504 Msa1186200:CDS
AATGTTTCAAGGGTGGTCTT+AGG 0.426325 8_1:-24267327 Msa1186200:three_prime_UTR
ACGTAAGGGTCAAATGATTA+GGG 0.438100 8_1:-24269541 Msa1186200:CDS
ATAATAACTCACCGTCTGAC+TGG 0.438965 8_1:+24267499 None:intergenic
CCTGATATAGTTAGAACAAA+AGG 0.440652 8_1:-24267707 Msa1186200:CDS
TAATGTGTTTGAAAGGTTAT+GGG 0.443792 8_1:-24268596 Msa1186200:CDS
CAAGAACATTTAAGGTCAAT+CGG 0.446659 8_1:+24268097 None:intergenic
TGGCCATGGTACCATACATC+TGG 0.449552 8_1:+24269211 None:intergenic
CAACCATACATATGTCTCTT+CGG 0.455896 8_1:+24268831 None:intergenic
CGAAATACTGCGGTGATGGT+AGG 0.457326 8_1:-24268147 Msa1186200:CDS
CTCACGCTTTCGGTTCTCCC+TGG 0.457913 8_1:+24269446 None:intergenic
GTCAATCGGTTCTTCGCAAA+AGG 0.459105 8_1:+24268111 None:intergenic
ATTTGCCTCCTCTTCAGATT+CGG 0.459174 8_1:+24269746 None:intergenic
GCAATTGTGTTCAACGATAT+GGG 0.462867 8_1:-24269687 Msa1186200:CDS
ATGCAAACTTGCAGCATTTG+TGG 0.464177 8_1:-24267641 Msa1186200:CDS
TCTAGTAATGCAATCGTCTC+TGG 0.474124 8_1:-24269807 Msa1186200:CDS
TTCTAACTATATCAGGATCA+AGG 0.475647 8_1:+24267714 None:intergenic
CAGTCACTTTCTCCGGGGAA+TGG 0.477886 8_1:-24267563 Msa1186200:CDS
TATATTGTATTATAGGATGT+TGG 0.479289 8_1:-24267397 Msa1186200:intron
TCACTCATCTTTCTGTATGC+AGG 0.481214 8_1:+24269285 None:intergenic
TGAAACAAGATGTGGATGTA+AGG 0.482330 8_1:-24269415 Msa1186200:CDS
AGAGACATATGTATGGTTGT+TGG 0.487097 8_1:-24268827 Msa1186200:CDS
CAGATGTGCAAAGTTGTTGC+TGG 0.487488 8_1:-24267878 Msa1186200:CDS
AGAGCCTCAAACAAAAGATC+TGG 0.487748 8_1:+24270338 None:intergenic
ACATAGTCGGTGATGCGCAT+TGG 0.489812 8_1:+24268711 None:intergenic
TTGTTGGGTGGGACTTTGTG+TGG 0.489818 8_1:-24269315 Msa1186200:CDS
TGAAGGGTTTAGAGAGGATA+GGG 0.494161 8_1:-24269475 Msa1186200:CDS
ATAAGACCATTCCAGATGTA+TGG 0.497294 8_1:-24269222 Msa1186200:CDS
GATATAATGAAGTTTGATAT+GGG 0.498214 8_1:-24269663 Msa1186200:CDS
CCCGCCTGCTGCATTGGCCA+TGG 0.498998 8_1:+24269197 None:intergenic
TTGCCTTTGATGCAACCTAC+AGG 0.499410 8_1:-24268948 Msa1186200:CDS
GCAGGCGGGTTTCACAAGGT+TGG 0.500449 8_1:-24269183 Msa1186200:CDS
GGTGTTCAGTCACTTTCTCC+GGG 0.500522 8_1:-24267569 Msa1186200:CDS
CATGCGTCTGCGCCTGCCTG+GGG 0.501531 8_1:+24267661 None:intergenic
AATGTCAAGAAATGTCTTCT+GGG 0.501660 8_1:-24268186 Msa1186200:CDS
ACACCGGAGAAAATAACAAA+TGG 0.501771 8_1:+24268907 None:intergenic
GGTACCATGGCCAATGCAGC+AGG 0.502246 8_1:-24269201 Msa1186200:CDS
TTTCCTGTAGGTTGCATCAA+AGG 0.502863 8_1:+24268945 None:intergenic
TGATCATAACCATGAGTTGT+TGG 0.502949 8_1:-24269331 Msa1186200:CDS
CGTTTGTATGTTCATGTGAA+GGG 0.506489 8_1:-24269491 Msa1186200:CDS
AAGGATTTGTATAACCAAGT+TGG 0.513872 8_1:-24269150 Msa1186200:CDS
GATCATAACCATGAGTTGTT+GGG 0.514350 8_1:-24269330 Msa1186200:CDS
TAATAACTCACCGTCTGACT+GGG 0.525259 8_1:+24267500 None:intergenic
GTTGGTTTGCTAGAATGTCT+GGG 0.525952 8_1:-24269572 Msa1186200:CDS
TTCGGTAGTTTCGGCAGGCA+GGG 0.527195 8_1:+24269764 None:intergenic
ATTGAACGCAATAGAAAAGC+AGG 0.527313 8_1:-24269246 Msa1186200:CDS
TTTGCACATCTGTACTATAC+CGG 0.527550 8_1:+24267889 None:intergenic
CTTTGCGCTTGATTTACTCT+CGG 0.527629 8_1:+24270276 None:intergenic
ACGTTTGTATGTTCATGTGA+AGG 0.528222 8_1:-24269492 Msa1186200:CDS
GTTTCGAAATACTGCGGTGA+TGG 0.529413 8_1:-24268151 Msa1186200:CDS
GAAATACTGCGGTGATGGTA+GGG 0.530285 8_1:-24268146 Msa1186200:CDS
TAATAGCATTTCTCATTGCA+AGG 0.535165 8_1:+24268746 None:intergenic
CCATCGGTAATTACAGATAA+CGG 0.535350 8_1:+24268772 None:intergenic
ATTTCCTCTTCACCATTCCC+CGG 0.536043 8_1:+24267551 None:intergenic
CAAAATGTCCCATGTGGCTG+TGG 0.539256 8_1:+24268434 None:intergenic
AACTTCTGGAATGCCAATGT+TGG 0.540850 8_1:+24268660 None:intergenic
TTGTAGTGGTTCTGTGTACT+GGG 0.541523 8_1:-24267936 Msa1186200:CDS
CCACATCTTGTTTCATTACG+TGG 0.542170 8_1:+24269423 None:intergenic
TCGAGGTTAATGTGTTTGAA+AGG 0.542340 8_1:-24268603 Msa1186200:CDS
CGTAGACTGGTTTGTAACAC+AGG 0.543004 8_1:+24268304 None:intergenic
TCAGATTCGGTAGTTTCGGC+AGG 0.543494 8_1:+24269759 None:intergenic
AAACTTATTTCTGATGCAAG+TGG 0.544035 8_1:-24269114 Msa1186200:CDS
GTGTGTGACACAAACATAAG+TGG 0.545921 8_1:+24269054 None:intergenic
CCATGGCCAATGCAGCAGGC+GGG 0.549082 8_1:-24269197 Msa1186200:CDS
TAATACGACATTGCAGCGAA+TGG 0.549266 8_1:+24267279 None:intergenic
TAGACATTGTGTCGAAGCGT+TGG 0.549624 8_1:-24269374 Msa1186200:CDS
TCAATTGGTATCAGTACCCC+AGG 0.551710 8_1:-24267677 Msa1186200:CDS
TGAAAGGTTATGGGCTGAGA+TGG 0.552089 8_1:-24268587 Msa1186200:CDS
ACTTTGTTCAACAATTCCAC+AGG 0.555098 8_1:-24268387 Msa1186200:CDS
AGACCGAAGAGACATATGTA+TGG 0.558869 8_1:-24268834 Msa1186200:CDS
ACCATGGCCAATGCAGCAGG+CGG 0.562388 8_1:-24269198 Msa1186200:CDS
CACACAAGATTGAATGCAGC+GGG 0.562481 8_1:-24267374 Msa1186200:exon
TCATTTCTGGAATGTTTCAA+GGG 0.564420 8_1:-24267337 Msa1186200:three_prime_UTR
TTTCTGGAATGTTTCAAGGG+TGG 0.564566 8_1:-24267334 Msa1186200:three_prime_UTR
CTAGGCAAAACCAAACAATT+GGG 0.566282 8_1:+24267998 None:intergenic
GTGGGCCACTGCTCACATCA+GGG 0.566297 8_1:-24268503 Msa1186200:CDS
AAATGATTAGGGATCGTCAA+GGG 0.566337 8_1:-24269530 Msa1186200:CDS
CCGTTATCTGTAATTACCGA+TGG 0.566643 8_1:-24268772 Msa1186200:CDS
TGTTAGTGTGTTGCAATGTA+CGG 0.569919 8_1:-24267073 Msa1186200:three_prime_UTR
GAAGGAGAACATGGTTCAGA+AGG 0.570317 8_1:-24269714 Msa1186200:CDS
AAGTCCCACCCAACAACTCA+TGG 0.571261 8_1:+24269322 None:intergenic
TTGGTATCAGTACCCCAGGC+AGG 0.572307 8_1:-24267673 Msa1186200:CDS
AACCACTACAATATTTCTCA+CGG 0.574719 8_1:+24267948 None:intergenic
AAACAATCGTCTGGTTATGG+TGG 0.579159 8_1:+24268881 None:intergenic
GTGAAGGGTTTAGAGAGGAT+AGG 0.583533 8_1:-24269476 Msa1186200:CDS
TCTCATTGCAAGGTCTCCAT+CGG 0.585177 8_1:+24268756 None:intergenic
ATTTGTATAACCAAGTTGGT+AGG 0.585819 8_1:-24269146 Msa1186200:CDS
TTCATGTGAAGGGTTTAGAG+AGG 0.585894 8_1:-24269481 Msa1186200:CDS
TGCAGCAGGCGGGTTTCACA+AGG 0.587440 8_1:-24269187 Msa1186200:CDS
CGAAACTACCGAATCTGAAG+AGG 0.587554 8_1:-24269754 Msa1186200:CDS
TGTTTAAGAATGGAGTCACT+CGG 0.588048 8_1:-24268076 Msa1186200:CDS
TCTCTCAATAGAGCGTAGAC+TGG 0.590244 8_1:+24268291 None:intergenic
ATAGTTAGAACAAAAGGTTG+TGG 0.592906 8_1:-24267701 Msa1186200:CDS
ATGGTACCATACATCTGGAA+TGG 0.593554 8_1:+24269216 None:intergenic
AGTTATGGATCGTAGATCAG+AGG 0.597822 8_1:-24270025 Msa1186200:exon
ATCCGTGAGAAATATTGTAG+TGG 0.598126 8_1:-24267950 Msa1186200:CDS
AACTACCGAATCTGAAGAGG+AGG 0.598164 8_1:-24269751 Msa1186200:CDS
CGAAACGCATCGTCCAACAT+TGG 0.602477 8_1:-24268673 Msa1186200:CDS
CCAATATTTAAATTTAGGCG+TGG 0.603258 8_1:+24267590 None:intergenic
CTGGTTATGGTGGTTAACAC+CGG 0.604355 8_1:+24268891 None:intergenic
CGCATCACCGACTATGTGCG+TGG 0.607731 8_1:-24268705 Msa1186200:CDS
AATGCAGCGGGACTATGCTA+AGG 0.609893 8_1:-24267362 Msa1186200:three_prime_UTR
AAACCAACAATAGAACATGT+AGG 0.609954 8_1:+24269587 None:intergenic
TGAGGCGCTCCACAGCCACA+TGG 0.610207 8_1:-24268443 Msa1186200:CDS
ACTGCGGTGATGGTAGGGAA+TGG 0.612310 8_1:-24268141 Msa1186200:CDS
TTCTCCGGGGAATGGTGAAG+AGG 0.618817 8_1:-24267555 Msa1186200:CDS
GAGGCGCTCCACAGCCACAT+GGG 0.620737 8_1:-24268442 Msa1186200:CDS
GAAGGGTTTAGAGAGGATAG+GGG 0.622672 8_1:-24269474 Msa1186200:CDS
ATCAAGCGCAAAGATTTCCA+AGG 0.623234 8_1:-24270266 Msa1186200:intron
ATTCCAGATGTATGGTACCA+TGG 0.623771 8_1:-24269214 Msa1186200:CDS
AAGGGTTTAGAGAGGATAGG+GGG 0.623970 8_1:-24269473 Msa1186200:CDS
AGATGTGCAAAGTTGTTGCT+GGG 0.628468 8_1:-24267877 Msa1186200:CDS
TCGAACGACCTCTCGTTGTG+AGG 0.629998 8_1:-24268461 Msa1186200:CDS
GCATACAGAAAGATGAGTGA+AGG 0.633024 8_1:-24269282 Msa1186200:CDS
TGTAGGTTGCATCAAAGGCA+AGG 0.633101 8_1:+24268950 None:intergenic
AGCAGCAAAGTTGTTGACGA+AGG 0.633585 8_1:-24268266 Msa1186200:CDS
TGTCTCACTGAGTCTAGTAG+TGG 0.634231 8_1:+24267182 None:intergenic
GAAAATAAGTTAGACACCTG+TGG 0.639120 8_1:+24268371 None:intergenic
CTACAACTTCCCCAGTCAGA+CGG 0.639517 8_1:-24267510 Msa1186200:intron
TTTGTGTGGATGATGTTGGA+AGG 0.640334 8_1:-24269635 Msa1186200:CDS
CATAACCATGAGTTGTTGGG+TGG 0.647455 8_1:-24269327 Msa1186200:CDS
GTTATGGATCGTAGATCAGA+GGG 0.651862 8_1:-24270024 Msa1186200:CDS
TGTGTGACACAAACATAAGT+GGG 0.652228 8_1:+24269055 None:intergenic
ACAAGTGCCACGCACATAGT+CGG 0.652480 8_1:+24268698 None:intergenic
TTTAAGGTTTCGAAATACTG+CGG 0.652628 8_1:-24268157 Msa1186200:CDS
TATTTCTGATGCAAGTGGTG+CGG 0.653017 8_1:-24269109 Msa1186200:CDS
TGGGCCACTGCTCACATCAG+GGG 0.653776 8_1:-24268502 Msa1186200:CDS
ACTTCCCCTGATGTGAGCAG+TGG 0.655038 8_1:+24268498 None:intergenic
GTGGAGCGCCTCACAACGAG+AGG 0.655392 8_1:+24268453 None:intergenic
CAATTCTTAAGTGCTATGAA+AGG 0.655915 8_1:-24268802 Msa1186200:CDS
TGTAGTAATATGTCAAGTGG+TGG 0.659670 8_1:-24269831 Msa1186200:CDS
TTTGCGAATTTCGACCACCT+AGG 0.664866 8_1:+24267980 None:intergenic
AATGATTAGGGATCGTCAAG+GGG 0.667751 8_1:-24269529 Msa1186200:CDS
ATAACCATGAGTTGTTGGGT+GGG 0.671128 8_1:-24269326 Msa1186200:CDS
GCACACAAGATTGAATGCAG+CGG 0.681885 8_1:-24267375 Msa1186200:exon
CAAATGATTAGGGATCGTCA+AGG 0.687921 8_1:-24269531 Msa1186200:CDS
AATAACTCACCGTCTGACTG+GGG 0.696426 8_1:+24267501 None:intergenic
GATTGTAGTAATATGTCAAG+TGG 0.697996 8_1:-24269834 Msa1186200:CDS
GTGTTCAGTCACTTTCTCCG+GGG 0.698911 8_1:-24267568 Msa1186200:CDS
CCACGTAATGAAACAAGATG+TGG 0.700849 8_1:-24269423 Msa1186200:CDS
TTTCAAACACATTAACCTCG+AGG 0.704126 8_1:+24268605 None:intergenic
ACAGATATGATATGATCACA+TGG 0.719879 8_1:+24268049 None:intergenic
ATGGATCGTAGATCAGAGGG+TGG 0.722528 8_1:-24270021 Msa1186200:CDS
ATAGTCGGTGATGCGCATTG+GGG 0.752339 8_1:+24268713 None:intergenic
AATGCACAAAATGTCCCATG+TGG 0.793030 8_1:+24268428 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTTTTAAAATAATGA+AGG - chr8_1:24267274-24267293 Msa1186200:three_prime_UTR 10.0%
!! AATGAATTATATTGTATTAT+AGG - chr8_1:24269995-24270014 Msa1186200:CDS 10.0%
!! AACAATAAATAAATAAGCTT+CGG + chr8_1:24267261-24267280 None:intergenic 15.0%
!!! GATTTAATTTGTATGTAAAA+TGG - chr8_1:24267494-24267513 Msa1186200:intron 15.0%
!! AATGATTAATCTAAACAGAT+CGG + chr8_1:24267200-24267219 None:intergenic 20.0%
!! TGATATAATGAAGTTTGATA+TGG - chr8_1:24267735-24267754 Msa1186200:CDS 20.0%
!! GATATAATGAAGTTTGATAT+GGG - chr8_1:24267736-24267755 Msa1186200:CDS 20.0%
!!! AAGCTTTTTATTACTATCAA+GGG + chr8_1:24268370-24268389 None:intergenic 20.0%
!! TTATCTTAAAAAATGCATGT+TGG - chr8_1:24268767-24268786 Msa1186200:CDS 20.0%
!! TATCTTAAAAAATGCATGTT+GGG - chr8_1:24268768-24268787 Msa1186200:CDS 20.0%
!! TTCTTAAACAAGAACATTTA+AGG + chr8_1:24269313-24269332 None:intergenic 20.0%
!!! TAAATGTTCTTGTTTAAGAA+TGG - chr8_1:24269313-24269332 Msa1186200:CDS 20.0%
!!! TGTTCTTTAATTTTAATCTC+TGG + chr8_1:24269607-24269626 None:intergenic 20.0%
!! GAACACCAATATTTAAATTT+AGG + chr8_1:24269817-24269836 None:intergenic 20.0%
!! TATATTGTATTATAGGATGT+TGG - chr8_1:24270002-24270021 Msa1186200:CDS 20.0%
!!! TATGTATCATGTTTTGTAAT+AGG - chr8_1:24270279-24270298 Msa1186200:five_prime_UTR 20.0%
! GGAAGAATAAAATGCAATAT+GGG + chr8_1:24267163-24267182 None:intergenic 25.0%
! AGGAAGAATAAAATGCAATA+TGG + chr8_1:24267164-24267183 None:intergenic 25.0%
! ATGATTAATCTAAACAGATC+GGG + chr8_1:24267199-24267218 None:intergenic 25.0%
! GATGAAATTTGATGTTCTTT+AGG - chr8_1:24267346-24267365 Msa1186200:three_prime_UTR 25.0%
! CAATTCAAATCATGTCAAAT+TGG + chr8_1:24267475-24267494 None:intergenic 25.0%
!! AAGGTATGATTTTGTTAGTT+TGG - chr8_1:24267783-24267802 Msa1186200:CDS 25.0%
!! CAAGCTTTTTATTACTATCA+AGG + chr8_1:24268371-24268390 None:intergenic 25.0%
!!! GTTATATTTGTTTTTCCTGT+AGG + chr8_1:24268469-24268488 None:intergenic 25.0%
! TTAAGATAAGACATAACTTC+TGG + chr8_1:24268756-24268775 None:intergenic 25.0%
! ATCTTAAAAAATGCATGTTG+GGG - chr8_1:24268769-24268788 Msa1186200:CDS 25.0%
! TTAATGTGTTTGAAAGGTTA+TGG - chr8_1:24268802-24268821 Msa1186200:CDS 25.0%
! TAATGTGTTTGAAAGGTTAT+GGG - chr8_1:24268803-24268822 Msa1186200:CDS 25.0%
! TGTATGAGAAAAAAAACATG+TGG - chr8_1:24268877-24268896 Msa1186200:CDS 25.0%
! GTATGAGAAAAAAAACATGT+GGG - chr8_1:24268878-24268897 Msa1186200:CDS 25.0%
!! TCTGATTTTCAATCAAACTA+TGG - chr8_1:24269068-24269087 Msa1186200:CDS 25.0%
!! AAAACCAGATCTTTTGTTTG+AGG - chr8_1:24267057-24267076 Msa1186200:three_prime_UTR 30.0%
!!! GTTCTTTAGGTTTTGAGTTA+TGG - chr8_1:24267359-24267378 Msa1186200:three_prime_UTR 30.0%
GATTGTAGTAATATGTCAAG+TGG - chr8_1:24267565-24267584 Msa1186200:CDS 30.0%
AAACCAACAATAGAACATGT+AGG + chr8_1:24267815-24267834 None:intergenic 30.0%
AAGGATTTGTATAACCAAGT+TGG - chr8_1:24268249-24268268 Msa1186200:CDS 30.0%
!! ATTTGTATAACCAAGTTGGT+AGG - chr8_1:24268253-24268272 Msa1186200:CDS 30.0%
!! AAACTTATTTCTGATGCAAG+TGG - chr8_1:24268285-24268304 Msa1186200:CDS 30.0%
! AAAAGCTTGAGAGATTGTTT+TGG - chr8_1:24268382-24268401 Msa1186200:CDS 30.0%
!!! TGTCCATTTGTTATTTTCTC+CGG - chr8_1:24268489-24268508 Msa1186200:CDS 30.0%
CAATTCTTAAGTGCTATGAA+AGG - chr8_1:24268597-24268616 Msa1186200:CDS 30.0%
TAATAGCATTTCTCATTGCA+AGG + chr8_1:24268656-24268675 None:intergenic 30.0%
TCTTAAAAAATGCATGTTGG+GGG - chr8_1:24268770-24268789 Msa1186200:CDS 30.0%
TTGGTTTAGAAGACAACAAT+TGG - chr8_1:24268844-24268863 Msa1186200:CDS 30.0%
!! TCTAACTTATTTTCGCTTCA+GGG - chr8_1:24269037-24269056 Msa1186200:CDS 30.0%
! AGGAAATCTTTTTCTTGTTC+AGG - chr8_1:24269153-24269172 Msa1186200:CDS 30.0%
! AATCTTTTTCTTGTTCAGGT+CGG - chr8_1:24269157-24269176 Msa1186200:CDS 30.0%
AATGTCAAGAAATGTCTTCT+GGG - chr8_1:24269213-24269232 Msa1186200:CDS 30.0%
! TTTAAGGTTTCGAAATACTG+CGG - chr8_1:24269242-24269261 Msa1186200:CDS 30.0%
! CAAGAACATTTAAGGTCAAT+CGG + chr8_1:24269305-24269324 None:intergenic 30.0%
ACAGATATGATATGATCACA+TGG + chr8_1:24269353-24269372 None:intergenic 30.0%
AACCACTACAATATTTCTCA+CGG + chr8_1:24269454-24269473 None:intergenic 30.0%
TTCTAACTATATCAGGATCA+AGG + chr8_1:24269688-24269707 None:intergenic 30.0%
! CCTTTTGTTCTAACTATATC+AGG + chr8_1:24269695-24269714 None:intergenic 30.0%
CCTGATATAGTTAGAACAAA+AGG - chr8_1:24269692-24269711 Msa1186200:CDS 30.0%
ATAGTTAGAACAAAAGGTTG+TGG - chr8_1:24269698-24269717 Msa1186200:CDS 30.0%
CCAATATTTAAATTTAGGCG+TGG + chr8_1:24269812-24269831 None:intergenic 30.0%
CCACGCCTAAATTTAAATAT+TGG - chr8_1:24269809-24269828 Msa1186200:CDS 30.0%
!! TCATTTCTGGAATGTTTCAA+GGG - chr8_1:24270062-24270081 Msa1186200:intron 30.0%
AATGCAATATGGGATTACCT+TGG + chr8_1:24267153-24267172 None:intergenic 35.0%
!! AGATCGGGTTGATTTATTAC+AGG + chr8_1:24267184-24267203 None:intergenic 35.0%
TCAACAGATTTCAGAGTAGT+TGG + chr8_1:24267231-24267250 None:intergenic 35.0%
!!! AATCAGTTGTTTTTGGATGC+AGG - chr8_1:24267402-24267421 Msa1186200:intron 35.0%
ATCATGTCAAATTGGAAAGC+CGG + chr8_1:24267467-24267486 None:intergenic 35.0%
! TACAGCTTGCATACTTTTAC+AGG - chr8_1:24267525-24267544 Msa1186200:CDS 35.0%
TGTAGTAATATGTCAAGTGG+TGG - chr8_1:24267568-24267587 Msa1186200:CDS 35.0%
AGCAATTGTGTTCAACGATA+TGG - chr8_1:24267711-24267730 Msa1186200:CDS 35.0%
GCAATTGTGTTCAACGATAT+GGG - chr8_1:24267712-24267731 Msa1186200:CDS 35.0%
! TGATATGGGAAACTTTTGTG+TGG - chr8_1:24267750-24267769 Msa1186200:CDS 35.0%
! AACTTTTGTGTGGATGATGT+TGG - chr8_1:24267760-24267779 Msa1186200:CDS 35.0%
TTGCCTACATGTTCTATTGT+TGG - chr8_1:24267809-24267828 Msa1186200:CDS 35.0%
TACGTAAGGGTCAAATGATT+AGG - chr8_1:24267857-24267876 Msa1186200:CDS 35.0%
ACGTAAGGGTCAAATGATTA+GGG - chr8_1:24267858-24267877 Msa1186200:CDS 35.0%
AAATGATTAGGGATCGTCAA+GGG - chr8_1:24267869-24267888 Msa1186200:CDS 35.0%
ACGTTTGTATGTTCATGTGA+AGG - chr8_1:24267907-24267926 Msa1186200:CDS 35.0%
CGTTTGTATGTTCATGTGAA+GGG - chr8_1:24267908-24267927 Msa1186200:CDS 35.0%
TGAAACAAGATGTGGATGTA+AGG - chr8_1:24267984-24268003 Msa1186200:CDS 35.0%
TGATCATAACCATGAGTTGT+TGG - chr8_1:24268068-24268087 Msa1186200:CDS 35.0%
GATCATAACCATGAGTTGTT+GGG - chr8_1:24268069-24268088 Msa1186200:CDS 35.0%
ATTGAACGCAATAGAAAAGC+AGG - chr8_1:24268153-24268172 Msa1186200:CDS 35.0%
ATAAGACCATTCCAGATGTA+TGG - chr8_1:24268177-24268196 Msa1186200:CDS 35.0%
CAAGGTTGGATTTGCAAAAA+AGG - chr8_1:24268230-24268249 Msa1186200:CDS 35.0%
TGTGTGACACAAACATAAGT+GGG + chr8_1:24268347-24268366 None:intergenic 35.0%
!!! AGAGATTGTTTTGGAGTGAT+GGG - chr8_1:24268391-24268410 Msa1186200:CDS 35.0%
CGAATGAACTACGAGATATT+TGG - chr8_1:24268420-24268439 Msa1186200:CDS 35.0%
ACACCGGAGAAAATAACAAA+TGG + chr8_1:24268495-24268514 None:intergenic 35.0%
CAAAAACAATCGTCTGGTTA+TGG + chr8_1:24268524-24268543 None:intergenic 35.0%
! CATAACCAGACGATTGTTTT+TGG - chr8_1:24268522-24268541 Msa1186200:CDS 35.0%
CAACCATACATATGTCTCTT+CGG + chr8_1:24268571-24268590 None:intergenic 35.0%
AGAGACATATGTATGGTTGT+TGG - chr8_1:24268572-24268591 Msa1186200:CDS 35.0%
CCATCGGTAATTACAGATAA+CGG + chr8_1:24268630-24268649 None:intergenic 35.0%
TTTCAAACACATTAACCTCG+AGG + chr8_1:24268797-24268816 None:intergenic 35.0%
TCGAGGTTAATGTGTTTGAA+AGG - chr8_1:24268796-24268815 Msa1186200:CDS 35.0%
ACTTTGTTCAACAATTCCAC+AGG - chr8_1:24269012-24269031 Msa1186200:CDS 35.0%
GAAAATAAGTTAGACACCTG+TGG + chr8_1:24269031-24269050 None:intergenic 35.0%
!! GTCTAACTTATTTTCGCTTC+AGG - chr8_1:24269036-24269055 Msa1186200:CDS 35.0%
GAATGTCAAGAAATGTCTTC+TGG - chr8_1:24269212-24269231 Msa1186200:CDS 35.0%
TGTTTAAGAATGGAGTCACT+CGG - chr8_1:24269323-24269342 Msa1186200:CDS 35.0%
CTAGGCAAAACCAAACAATT+GGG + chr8_1:24269404-24269423 None:intergenic 35.0%
ATCCGTGAGAAATATTGTAG+TGG - chr8_1:24269449-24269468 Msa1186200:CDS 35.0%
TTTGCACATCTGTACTATAC+CGG + chr8_1:24269513-24269532 None:intergenic 35.0%
! ATTATCTCCAGTTTTGCAGA+CGG - chr8_1:24269643-24269662 Msa1186200:CDS 35.0%
ACAAAAGGTTGTGGTTCAAT+TGG - chr8_1:24269707-24269726 Msa1186200:CDS 35.0%
GTGGACAACATCGTTTATTA+TGG + chr8_1:24269793-24269812 None:intergenic 35.0%
!! GTCATTTCTGGAATGTTTCA+AGG - chr8_1:24270061-24270080 Msa1186200:intron 35.0%
GTAATAGGATGCTTGTGATT+TGG - chr8_1:24270294-24270313 Msa1186200:five_prime_UTR 35.0%
TGTTAGTGTGTTGCAATGTA+CGG - chr8_1:24270326-24270345 Msa1186200:five_prime_UTR 35.0%
AGAGCCTCAAACAAAAGATC+TGG + chr8_1:24267064-24267083 None:intergenic 40.0%
!! TCTTTTGTTTGAGGCTCTTG+TGG - chr8_1:24267066-24267085 Msa1186200:three_prime_UTR 40.0%
!! CTTTTGTTTGAGGCTCTTGT+GGG - chr8_1:24267067-24267086 Msa1186200:three_prime_UTR 40.0%
CTTTGCGCTTGATTTACTCT+CGG + chr8_1:24267126-24267145 None:intergenic 40.0%
ATCAAGCGCAAAGATTTCCA+AGG - chr8_1:24267133-24267152 Msa1186200:three_prime_UTR 40.0%
AGTTATGGATCGTAGATCAG+AGG - chr8_1:24267374-24267393 Msa1186200:exon 40.0%
GTTATGGATCGTAGATCAGA+GGG - chr8_1:24267375-24267394 Msa1186200:exon 40.0%
!! GGGTGGAAATCAGTTGTTTT+TGG - chr8_1:24267395-24267414 Msa1186200:intron 40.0%
!!! TTGTTTTTGGATGCAGGTCT+GGG - chr8_1:24267408-24267427 Msa1186200:intron 40.0%
GCATCTAGTACCATTGAATC+CGG - chr8_1:24267445-24267464 Msa1186200:intron 40.0%
AATTGGAAAGCCGGATTCAA+TGG + chr8_1:24267458-24267477 None:intergenic 40.0%
! CATACTTTTACAGGAGACGA+TGG - chr8_1:24267534-24267553 Msa1186200:CDS 40.0%
TCTAGTAATGCAATCGTCTC+TGG - chr8_1:24267592-24267611 Msa1186200:CDS 40.0%
! CTCTTCAGATTCGGTAGTTT+CGG + chr8_1:24267647-24267666 None:intergenic 40.0%
ATTTGCCTCCTCTTCAGATT+CGG + chr8_1:24267656-24267675 None:intergenic 40.0%
! GAGGCAAATTCTTTTGACGA+AGG - chr8_1:24267667-24267686 Msa1186200:CDS 40.0%
! TCTTTTGACGAAGGAGAACA+TGG - chr8_1:24267676-24267695 Msa1186200:CDS 40.0%
TTTGTGTGGATGATGTTGGA+AGG - chr8_1:24267764-24267783 Msa1186200:CDS 40.0%
!! TGTTGGTTTGCTAGAATGTC+TGG - chr8_1:24267826-24267845 Msa1186200:CDS 40.0%
!! GTTGGTTTGCTAGAATGTCT+GGG - chr8_1:24267827-24267846 Msa1186200:CDS 40.0%
! TCTGGGTTTTCTGTACGTAA+GGG - chr8_1:24267844-24267863 Msa1186200:CDS 40.0%
CAAATGATTAGGGATCGTCA+AGG - chr8_1:24267868-24267887 Msa1186200:CDS 40.0%
AATGATTAGGGATCGTCAAG+GGG - chr8_1:24267870-24267889 Msa1186200:CDS 40.0%
TTCATGTGAAGGGTTTAGAG+AGG - chr8_1:24267918-24267937 Msa1186200:CDS 40.0%
TGAAGGGTTTAGAGAGGATA+GGG - chr8_1:24267924-24267943 Msa1186200:CDS 40.0%
! CCACATCTTGTTTCATTACG+TGG + chr8_1:24267979-24267998 None:intergenic 40.0%
CCACGTAATGAAACAAGATG+TGG - chr8_1:24267976-24267995 Msa1186200:CDS 40.0%
ATAACCATGAGTTGTTGGGT+GGG - chr8_1:24268073-24268092 Msa1186200:CDS 40.0%
TCACTCATCTTTCTGTATGC+AGG + chr8_1:24268117-24268136 None:intergenic 40.0%
GCATACAGAAAGATGAGTGA+AGG - chr8_1:24268117-24268136 Msa1186200:CDS 40.0%
ATGGTACCATACATCTGGAA+TGG + chr8_1:24268186-24268205 None:intergenic 40.0%
ATTCCAGATGTATGGTACCA+TGG - chr8_1:24268185-24268204 Msa1186200:CDS 40.0%
! TTTTACGTTGCCTACCAACT+TGG + chr8_1:24268266-24268285 None:intergenic 40.0%
! TATTTCTGATGCAAGTGGTG+CGG - chr8_1:24268290-24268309 Msa1186200:CDS 40.0%
GTGTGTGACACAAACATAAG+TGG + chr8_1:24268348-24268367 None:intergenic 40.0%
!!! GAGAGATTGTTTTGGAGTGA+TGG - chr8_1:24268390-24268409 Msa1186200:CDS 40.0%
!!! GAGATTGTTTTGGAGTGATG+GGG - chr8_1:24268392-24268411 Msa1186200:CDS 40.0%
TTTCCTGTAGGTTGCATCAA+AGG + chr8_1:24268457-24268476 None:intergenic 40.0%
AAACAATCGTCTGGTTATGG+TGG + chr8_1:24268521-24268540 None:intergenic 40.0%
CACTACCAAAAACAATCGTC+TGG + chr8_1:24268530-24268549 None:intergenic 40.0%
!!! CAGACGATTGTTTTTGGTAG+TGG - chr8_1:24268528-24268547 Msa1186200:CDS 40.0%
AGACCGAAGAGACATATGTA+TGG - chr8_1:24268565-24268584 Msa1186200:CDS 40.0%
CCGTTATCTGTAATTACCGA+TGG - chr8_1:24268627-24268646 Msa1186200:CDS 40.0%
!! AACTTCTGGAATGCCAATGT+TGG + chr8_1:24268742-24268761 None:intergenic 40.0%
!!! ATCAGGGGAAGTTTTTTTGC+TGG - chr8_1:24268912-24268931 Msa1186200:CDS 40.0%
AATGCACAAAATGTCCCATG+TGG + chr8_1:24268974-24268993 None:intergenic 40.0%
TGATGAGCTTCCCAATTGTT+TGG - chr8_1:24269391-24269410 Msa1186200:CDS 40.0%
CCTAGGCAAAACCAAACAAT+TGG + chr8_1:24269405-24269424 None:intergenic 40.0%
!!! CCAATTGTTTGGTTTTGCCT+AGG - chr8_1:24269402-24269421 Msa1186200:CDS 40.0%
!!! ATTGTTTGGTTTTGCCTAGG+TGG - chr8_1:24269405-24269424 Msa1186200:CDS 40.0%
! ATTGTAGTGGTTCTGTGTAC+TGG - chr8_1:24269462-24269481 Msa1186200:CDS 40.0%
! TTGTAGTGGTTCTGTGTACT+GGG - chr8_1:24269463-24269482 Msa1186200:CDS 40.0%
CTGTACTATACCGGAATATC+TGG + chr8_1:24269504-24269523 None:intergenic 40.0%
AGATGTGCAAAGTTGTTGCT+GGG - chr8_1:24269522-24269541 Msa1186200:CDS 40.0%
TCATTTACCGTCTGCAAAAC+TGG + chr8_1:24269653-24269672 None:intergenic 40.0%
! AGTTTTGCAGACGGTAAATG+AGG - chr8_1:24269652-24269671 Msa1186200:CDS 40.0%
ATGCAAACTTGCAGCATTTG+TGG - chr8_1:24269758-24269777 Msa1186200:CDS 40.0%
TGCAGCATTTGTGGTGTAAT+AGG - chr8_1:24269767-24269786 Msa1186200:CDS 40.0%
TAATAACTCACCGTCTGACT+GGG + chr8_1:24269902-24269921 None:intergenic 40.0%
ATAATAACTCACCGTCTGAC+TGG + chr8_1:24269903-24269922 None:intergenic 40.0%
CTATGCTAAGGAGTCATTTC+TGG - chr8_1:24270049-24270068 Msa1186200:intron 40.0%
!! TTTCTGGAATGTTTCAAGGG+TGG - chr8_1:24270065-24270084 Msa1186200:intron 40.0%
AATGTTTCAAGGGTGGTCTT+AGG - chr8_1:24270072-24270091 Msa1186200:intron 40.0%
TAATACGACATTGCAGCGAA+TGG + chr8_1:24270123-24270142 None:intergenic 40.0%
!!! GTTGTTTTTGGATGCAGGTC+TGG - chr8_1:24267407-24267426 Msa1186200:intron 45.0%
CGAAACTACCGAATCTGAAG+AGG - chr8_1:24267645-24267664 Msa1186200:CDS 45.0%
AACTACCGAATCTGAAGAGG+AGG - chr8_1:24267648-24267667 Msa1186200:CDS 45.0%
GAAGGAGAACATGGTTCAGA+AGG - chr8_1:24267685-24267704 Msa1186200:CDS 45.0%
! GTCTGGGTTTTCTGTACGTA+AGG - chr8_1:24267843-24267862 Msa1186200:CDS 45.0%
GTGAAGGGTTTAGAGAGGAT+AGG - chr8_1:24267923-24267942 Msa1186200:CDS 45.0%
GAAGGGTTTAGAGAGGATAG+GGG - chr8_1:24267925-24267944 Msa1186200:CDS 45.0%
AAGGGTTTAGAGAGGATAGG+GGG - chr8_1:24267926-24267945 Msa1186200:CDS 45.0%
TAGACATTGTGTCGAAGCGT+TGG - chr8_1:24268025-24268044 Msa1186200:CDS 45.0%
CATAACCATGAGTTGTTGGG+TGG - chr8_1:24268072-24268091 Msa1186200:CDS 45.0%
TGTAGGTTGCATCAAAGGCA+AGG + chr8_1:24268452-24268471 None:intergenic 45.0%
TTGCCTTTGATGCAACCTAC+AGG - chr8_1:24268451-24268470 Msa1186200:CDS 45.0%
CTGGTTATGGTGGTTAACAC+CGG + chr8_1:24268511-24268530 None:intergenic 45.0%
TCTCATTGCAAGGTCTCCAT+CGG + chr8_1:24268646-24268665 None:intergenic 45.0%
! TGAAAGGTTATGGGCTGAGA+TGG - chr8_1:24268812-24268831 Msa1186200:CDS 45.0%
GCTGAGATGGTTCAGAAGTT+TGG - chr8_1:24268825-24268844 Msa1186200:CDS 45.0%
!! CGTAGACTGGTTTGTAACAC+AGG + chr8_1:24269098-24269117 None:intergenic 45.0%
TCTCTCAATAGAGCGTAGAC+TGG + chr8_1:24269111-24269130 None:intergenic 45.0%
AGCAGCAAAGTTGTTGACGA+AGG - chr8_1:24269133-24269152 Msa1186200:CDS 45.0%
GTCTTCTGGGTGCATCTTTA+AGG - chr8_1:24269226-24269245 Msa1186200:CDS 45.0%
GTTTCGAAATACTGCGGTGA+TGG - chr8_1:24269248-24269267 Msa1186200:CDS 45.0%
! GAAATACTGCGGTGATGGTA+GGG - chr8_1:24269253-24269272 Msa1186200:CDS 45.0%
! GTCAATCGGTTCTTCGCAAA+AGG + chr8_1:24269291-24269310 None:intergenic 45.0%
TTTGCGAATTTCGACCACCT+AGG + chr8_1:24269422-24269441 None:intergenic 45.0%
CAATCTGCAGCCAGATATTC+CGG - chr8_1:24269491-24269510 Msa1186200:CDS 45.0%
CAGATGTGCAAAGTTGTTGC+TGG - chr8_1:24269521-24269540 Msa1186200:CDS 45.0%
TCAATTGGTATCAGTACCCC+AGG - chr8_1:24269722-24269741 Msa1186200:CDS 45.0%
! TGGTGTTCAGTCACTTTCTC+CGG - chr8_1:24269829-24269848 Msa1186200:CDS 45.0%
ATTTCCTCTTCACCATTCCC+CGG + chr8_1:24269851-24269870 None:intergenic 45.0%
AATAACTCACCGTCTGACTG+GGG + chr8_1:24269901-24269920 None:intergenic 45.0%
GCACACAAGATTGAATGCAG+CGG - chr8_1:24270024-24270043 Msa1186200:CDS 45.0%
CACACAAGATTGAATGCAGC+GGG - chr8_1:24270025-24270044 Msa1186200:exon 45.0%
TGTCTCACTGAGTCTAGTAG+TGG + chr8_1:24270220-24270239 None:intergenic 45.0%
ATGGATCGTAGATCAGAGGG+TGG - chr8_1:24267378-24267397 Msa1186200:exon 50.0%
! TCAGATTCGGTAGTTTCGGC+AGG + chr8_1:24267643-24267662 None:intergenic 50.0%
CATTACGTGGCTCACGCTTT+CGG + chr8_1:24267966-24267985 None:intergenic 50.0%
AAGTCCCACCCAACAACTCA+TGG + chr8_1:24268080-24268099 None:intergenic 50.0%
TTGTTGGGTGGGACTTTGTG+TGG - chr8_1:24268084-24268103 Msa1186200:CDS 50.0%
TGGCCATGGTACCATACATC+TGG + chr8_1:24268191-24268210 None:intergenic 50.0%
ATAGTCGGTGATGCGCATTG+GGG + chr8_1:24268689-24268708 None:intergenic 50.0%
CATAGTCGGTGATGCGCATT+GGG + chr8_1:24268690-24268709 None:intergenic 50.0%
ACATAGTCGGTGATGCGCAT+TGG + chr8_1:24268691-24268710 None:intergenic 50.0%
ACAAGTGCCACGCACATAGT+CGG + chr8_1:24268704-24268723 None:intergenic 50.0%
CGAAACGCATCGTCCAACAT+TGG - chr8_1:24268726-24268745 Msa1186200:CDS 50.0%
CAAAATGTCCCATGTGGCTG+TGG + chr8_1:24268968-24268987 None:intergenic 50.0%
! CGAAATACTGCGGTGATGGT+AGG - chr8_1:24269252-24269271 Msa1186200:CDS 50.0%
! GGTGTTCAGTCACTTTCTCC+GGG - chr8_1:24269830-24269849 Msa1186200:CDS 50.0%
! GTGTTCAGTCACTTTCTCCG+GGG - chr8_1:24269831-24269850 Msa1186200:CDS 50.0%
CTACAACTTCCCCAGTCAGA+CGG - chr8_1:24269889-24269908 Msa1186200:intron 50.0%
AATGCAGCGGGACTATGCTA+AGG - chr8_1:24270037-24270056 Msa1186200:intron 50.0%
TTCGGTAGTTTCGGCAGGCA+GGG + chr8_1:24267638-24267657 None:intergenic 55.0%
ATTCGGTAGTTTCGGCAGGC+AGG + chr8_1:24267639-24267658 None:intergenic 55.0%
AGAGGATAGGGGGCTAACCA+GGG - chr8_1:24267936-24267955 Msa1186200:CDS 55.0%
ACTTCCCCTGATGTGAGCAG+TGG + chr8_1:24268904-24268923 None:intergenic 55.0%
TCGAACGACCTCTCGTTGTG+AGG - chr8_1:24268938-24268957 Msa1186200:CDS 55.0%
! ACTGCGGTGATGGTAGGGAA+TGG - chr8_1:24269258-24269277 Msa1186200:CDS 55.0%
TTGGTATCAGTACCCCAGGC+AGG - chr8_1:24269726-24269745 Msa1186200:CDS 55.0%
! CAGTCACTTTCTCCGGGGAA+TGG - chr8_1:24269836-24269855 Msa1186200:CDS 55.0%
TTCTCCGGGGAATGGTGAAG+AGG - chr8_1:24269844-24269863 Msa1186200:CDS 55.0%
GAGAGGATAGGGGGCTAACC+AGG - chr8_1:24267935-24267954 Msa1186200:CDS 60.0%
CTCACGCTTTCGGTTCTCCC+TGG + chr8_1:24267956-24267975 None:intergenic 60.0%
GGTACCATGGCCAATGCAGC+AGG - chr8_1:24268198-24268217 Msa1186200:CDS 60.0%
ACCATGGCCAATGCAGCAGG+CGG - chr8_1:24268201-24268220 Msa1186200:CDS 60.0%
GTGAAACCCGCCTGCTGCAT+TGG + chr8_1:24268211-24268230 None:intergenic 60.0%
! TGCAGCAGGCGGGTTTCACA+AGG - chr8_1:24268212-24268231 Msa1186200:CDS 60.0%
! GCAGGCGGGTTTCACAAGGT+TGG - chr8_1:24268216-24268235 Msa1186200:CDS 60.0%
! CGCATCACCGACTATGTGCG+TGG - chr8_1:24268694-24268713 Msa1186200:CDS 60.0%
ATGCATGTTGGGGGACCTCG+AGG - chr8_1:24268779-24268798 Msa1186200:CDS 60.0%
TGTGGGCCACTGCTCACATC+AGG - chr8_1:24268895-24268914 Msa1186200:CDS 60.0%
GTGGGCCACTGCTCACATCA+GGG - chr8_1:24268896-24268915 Msa1186200:CDS 60.0%
TGGGCCACTGCTCACATCAG+GGG - chr8_1:24268897-24268916 Msa1186200:CDS 60.0%
CCATGGCCAATGCAGCAGGC+GGG - chr8_1:24268202-24268221 Msa1186200:CDS 65.0%
! GTGGAGCGCCTCACAACGAG+AGG + chr8_1:24268949-24268968 None:intergenic 65.0%
! TGAGGCGCTCCACAGCCACA+TGG - chr8_1:24268956-24268975 Msa1186200:CDS 65.0%
! GAGGCGCTCCACAGCCACAT+GGG - chr8_1:24268957-24268976 Msa1186200:CDS 65.0%
! CCCGCCTGCTGCATTGGCCA+TGG + chr8_1:24268205-24268224 None:intergenic 70.0%
CATGCGTCTGCGCCTGCCTG+GGG + chr8_1:24269741-24269760 None:intergenic 70.0%
GCATGCGTCTGCGCCTGCCT+GGG + chr8_1:24269742-24269761 None:intergenic 70.0%
TGCATGCGTCTGCGCCTGCC+TGG + chr8_1:24269743-24269762 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 24267047 24270374 24267047 ID=Msa1186200;Name=Msa1186200
chr8_1 mRNA 24267047 24270374 24267047 ID=Msa1186200-mRNA-1;Parent=Msa1186200;Name=Msa1186200-mRNA-1;_AED=0.12;_eAED=0.00;_QI=118|1|0.75|1|0.66|0.5|4|337|815
chr8_1 exon 24267047 24267404 24267047 ID=Msa1186200-mRNA-1:exon:8836;Parent=Msa1186200-mRNA-1
chr8_1 exon 24267511 24269874 24267511 ID=Msa1186200-mRNA-1:exon:8835;Parent=Msa1186200-mRNA-1
chr8_1 exon 24269981 24270053 24269981 ID=Msa1186200-mRNA-1:exon:8834;Parent=Msa1186200-mRNA-1
chr8_1 exon 24270267 24270374 24270267 ID=Msa1186200-mRNA-1:exon:8833;Parent=Msa1186200-mRNA-1
chr8_1 five_prime_UTR 24270267 24270374 24270267 ID=Msa1186200-mRNA-1:five_prime_utr;Parent=Msa1186200-mRNA-1
chr8_1 five_prime_UTR 24270044 24270053 24270044 ID=Msa1186200-mRNA-1:five_prime_utr;Parent=Msa1186200-mRNA-1
chr8_1 CDS 24269981 24270043 24269981 ID=Msa1186200-mRNA-1:cds;Parent=Msa1186200-mRNA-1
chr8_1 CDS 24267511 24269874 24267511 ID=Msa1186200-mRNA-1:cds;Parent=Msa1186200-mRNA-1
chr8_1 CDS 24267384 24267404 24267384 ID=Msa1186200-mRNA-1:cds;Parent=Msa1186200-mRNA-1
chr8_1 three_prime_UTR 24267047 24267383 24267047 ID=Msa1186200-mRNA-1:three_prime_utr;Parent=Msa1186200-mRNA-1
Gene Sequence

>Msa1186200

ATGGATCGTAGATCAGAGGGTGGAAATCAGTTGTTTTTGGATGCAGGTCTGGGTATCACAACTGAGACGATGGAAGAAAGTGATTGTAGTAATATGTCAAGTGGTGGATCTAGTAATGCAATCGTCTCTGGTAGTTCAAACTCACAAGTATCCCTGCCTGCCGAAACTACCGAATCTGAAGAGGAGGCAAATTCTTTTGACGAAGGAGAACATGGTTCAGAAGGCGAAGCAATTGTGTTCAACGATATGGGTGATATAATGAAGTTTGATATGGGAAACTTTTGTGTGGATGATGTTGGAAGGTATGATTTTGTTAGTTTGGATGTTGCCTACATGTTCTATTGTTGGTTTGCTAGAATGTCTGGGTTTTCTGTACGTAAGGGTCAAATGATTAGGGATCGTCAAGGGGTAGTTTTGCAGCAAACGTTTGTATGTTCATGTGAAGGGTTTAGAGAGGATAGGGGGCTAACCAGGGAGAACCGAAAGCGTGAGCCACGTAATGAAACAAGATGTGGATGTAAGGCAAAATTTAGAGTGCACGTAGACATTGTGTCGAAGCGTTGGTATGTCACTATGTTTGAAATTGATCATAACCATGAGTTGTTGGGTGGGACTTTGTGTGGATTACTTCCTGCATACAGAAAGATGAGTGAAGGTGATATTGATGAGATTGAACGCAATAGAAAAGCAGGTATAAGACCATTCCAGATGTATGGTACCATGGCCAATGCAGCAGGCGGGTTTCACAAGGTTGGATTTGCAAAAAAGGATTTGTATAACCAAGTTGGTAGGCAACGTAAAAAACTTATTTCTGATGCAAGTGGTGCGGTAAAGTATCTTAGAGATCTGAACTTGAAAGACCCACTTATGTTTGTGTCACACACCCTTGATAGTAATAAAAAGCTTGAGAGATTGTTTTGGAGTGATGGGGAAAGTCGAATGAACTACGAGATATTTGGAGATGTCCTTGCCTTTGATGCAACCTACAGGAAAAACAAATATAACTGTCCATTTGTTATTTTCTCCGGTGTTAACCACCATAACCAGACGATTGTTTTTGGTAGTGGTATTGTAACACGAGAGACCGAAGAGACATATGTATGGTTGTTGGAGCAATTCTTAAGTGCTATGAAAGGCAAGCAGCCGTTATCTGTAATTACCGATGGAGACCTTGCAATGAGAAATGCTATTAAGAAAATATTCCCCAATGCGCATCACCGACTATGTGCGTGGCACTTGTTGCGAAACGCATCGTCCAACATTGGCATTCCAGAAGTTATGTCTTATCTTAAAAAATGCATGTTGGGGGACCTCGAGGTTAATGTGTTTGAAAGGTTATGGGCTGAGATGGTTCAGAAGTTTGGTTTAGAAGACAACAATTGGATCAATGAGATGTATGAGAAAAAAAACATGTGGGCCACTGCTCACATCAGGGGAAGTTTTTTTGCTGGTATTCGAACGACCTCTCGTTGTGAGGCGCTCCACAGCCACATGGGACATTTTGTGCATTCTCGAATAAGCATGACTGACTTTGTTCAACAATTCCACAGGTGTCTAACTTATTTTCGCTTCAGGGAAATTGAATCTGATTTTCAATCAAACTATGGTCAACCTGTGTTACAAACCAGTCTACGCTCTATTGAGAGATCAGCAGCAAAGTTGTTGACGAAGGAAATCTTTTTCTTGTTCAGGTCGGTACTAAAGAAAGCATTGCTTCTCAGAATAAATGAATGTCAAGAAATGTCTTCTGGGTGCATCTTTAAGGTTTCGAAATACTGCGGTGATGGTAGGGAATGGCATGTTTCCTTTTGCGAAGAACCGATTGACCTTAAATGTTCTTGTTTAAGAATGGAGTCACTCGGATTACCATGTGATCATATCATATCTGTGTTGTTGTATCTTGATTTTGATGAGCTTCCCAATTGTTTGGTTTTGCCTAGGTGGTCGAAATTCGCAAAAGATTTAATCCGTGAGAAATATTGTAGTGGTTCTGTGTACTGGGATTCGCAATCTGCAGCCAGATATTCCGGTATAGTACAGATGTGCAAAGTTGTTGCTGGGCTAGTTTACGAAGATCTTGATGAGTATAATCATTTTGTTGAAAGTTTAAGTGCTGAAATCCAGAGATTAAAATTAAAGAACAATGTTGAAGAACGCGAATTATCTCCAGTTTTGCAGACGGTAAATGAGGATGATGAACTCCTTGATCCTGATATAGTTAGAACAAAAGGTTGTGGTTCAATTGGTATCAGTACCCCAGGCAGGCGCAGACGCATGCAAACTTGCAGCATTTGTGGTGTAATAGGCCATAATAAACGATGTTGTCCACGCCTAAATTTAAATATTGGTGTTCAGTCACTTTCTCCGGGGAATGGTGAAGAGGAAATCGAAGAAGACGATGTCAACTACAACTTCCCCAGTCAGACGGATGTTGGCACACAAGATTGA
Protein sequence

>Msa1186200

MDRRSEGGNQLFLDAGLGITTETMEESDCSNMSSGGSSNAIVSGSSNSQVSLPAETTESEEEANSFDEGEHGSEGEAIVFNDMGDIMKFDMGNFCVDDVGRYDFVSLDVAYMFYCWFARMSGFSVRKGQMIRDRQGVVLQQTFVCSCEGFREDRGLTRENRKREPRNETRCGCKAKFRVHVDIVSKRWYVTMFEIDHNHELLGGTLCGLLPAYRKMSEGDIDEIERNRKAGIRPFQMYGTMANAAGGFHKVGFAKKDLYNQVGRQRKKLISDASGAVKYLRDLNLKDPLMFVSHTLDSNKKLERLFWSDGESRMNYEIFGDVLAFDATYRKNKYNCPFVIFSGVNHHNQTIVFGSGIVTRETEETYVWLLEQFLSAMKGKQPLSVITDGDLAMRNAIKKIFPNAHHRLCAWHLLRNASSNIGIPEVMSYLKKCMLGDLEVNVFERLWAEMVQKFGLEDNNWINEMYEKKNMWATAHIRGSFFAGIRTTSRCEALHSHMGHFVHSRISMTDFVQQFHRCLTYFRFREIESDFQSNYGQPVLQTSLRSIERSAAKLLTKEIFFLFRSVLKKALLLRINECQEMSSGCIFKVSKYCGDGREWHVSFCEEPIDLKCSCLRMESLGLPCDHIISVLLYLDFDELPNCLVLPRWSKFAKDLIREKYCSGSVYWDSQSAARYSGIVQMCKVVAGLVYEDLDEYNHFVESLSAEIQRLKLKNNVEERELSPVLQTVNEDDELLDPDIVRTKGCGSIGISTPGRRRRMQTCSICGVIGHNKRCCPRLNLNIGVQSLSPGNGEEEIEEDDVNYNFPSQTDVGTQD*