AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1186450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTGGGTGTTTGTGGATC+TGG 0.254382 8_1:+24747065 Msa1186450:CDS
AATCAACGTGTTTGAAGCTT+TGG 0.333073 8_1:+24747049 Msa1186450:CDS
ATCAACGTGTTTGAAGCTTT+GGG 0.394015 8_1:+24747050 Msa1186450:CDS
TATGAAGACTAGATACTTGA+TGG 0.414163 8_1:-24747204 None:intergenic
CTTTGGGTGTTTGTGGATCT+GGG 0.424338 8_1:+24747066 Msa1186450:CDS
TTCACTTCAAGAACGAAGCT+TGG 0.445881 8_1:+24747162 Msa1186450:CDS
TTTGAAGCTTTGGGTGTTTG+TGG 0.454771 8_1:+24747059 Msa1186450:CDS
GAAGACTAGATACTTGATGG+AGG 0.696684 8_1:-24747201 None:intergenic
ACTTGATGGAGGACACAGAG+AGG 0.696959 8_1:-24747190 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTTATTAAAAAAAAAT+TGG + chr8_1:24747273-24747292 Msa1186450:intron 0.0%
!! ATTTATTAAAAAAAAATTGG+TGG + chr8_1:24747276-24747295 Msa1186450:intron 10.0%
!! TTTATTAAAAAAAAATTGGT+GGG + chr8_1:24747277-24747296 Msa1186450:intron 10.0%
!!! AACCTTAATTTTTTTTTTTA+TGG + chr8_1:24748809-24748828 Msa1186450:intron 10.0%
!! AACCATAAAAAAAAAAATTA+AGG - chr8_1:24748814-24748833 None:intergenic 10.0%
!! AAATAAGAATGAAGTAAAAA+GGG - chr8_1:24747251-24747270 None:intergenic 15.0%
!!! AAACTTTGATTTTTTTTACT+AGG - chr8_1:24747550-24747569 None:intergenic 15.0%
!! ATTTATAATACCCAAATAAA+CGG - chr8_1:24747869-24747888 None:intergenic 15.0%
!!! TTGTATTTTTTAGTAGTTTT+TGG + chr8_1:24747941-24747960 Msa1186450:intron 15.0%
!! TATTTAATTATTTGTTTCCT+AGG - chr8_1:24747977-24747996 None:intergenic 15.0%
!! CAAAAAAATTTGAAAATTTC+AGG - chr8_1:24748001-24748020 None:intergenic 15.0%
!!! CTGAAATTTTCAAATTTTTT+TGG + chr8_1:24747999-24748018 Msa1186450:intron 15.0%
!! TTTACACAATTTATTCTAAT+AGG - chr8_1:24749080-24749099 None:intergenic 15.0%
!! AATATTCAAAATATAATGTG+AGG - chr8_1:24749603-24749622 None:intergenic 15.0%
!! GAAATAAGAATGAAGTAAAA+AGG - chr8_1:24747252-24747271 None:intergenic 20.0%
!!! AATATATTTGCCGTTTATTT+GGG + chr8_1:24747856-24747875 Msa1186450:intron 20.0%
!!! TATTTTTTAGTAGTTTTTGG+AGG + chr8_1:24747944-24747963 Msa1186450:intron 20.0%
!! GGCTCAATTAAAAAAAAAAT+TGG + chr8_1:24748020-24748039 Msa1186450:intron 20.0%
!! CAAAAACAAATAAATAGTCA+TGG - chr8_1:24748440-24748459 None:intergenic 20.0%
!!! CATGACTATTTATTTGTTTT+TGG + chr8_1:24748438-24748457 Msa1186450:intron 20.0%
!!! GAAATTATTTTAAGATCTCT+TGG + chr8_1:24748658-24748677 Msa1186450:intron 20.0%
!! CATAATACAAATTAGTCAAA+GGG - chr8_1:24748693-24748712 None:intergenic 20.0%
!! ATATCTCAAGAATAAAGTAT+CGG + chr8_1:24748915-24748934 Msa1186450:intron 20.0%
!! TTACCATATTTATAGACAAT+AGG - chr8_1:24749043-24749062 None:intergenic 20.0%
!!! CTTTTAAGTTAGATGAATTT+AGG + chr8_1:24749337-24749356 Msa1186450:intron 20.0%
!! CAAAATAAAACGAAATACAA+AGG - chr8_1:24749448-24749467 None:intergenic 20.0%
!!! ATAGTTGGGTTTTGTTTTTT+GGG + chr8_1:24747743-24747762 Msa1186450:intron 25.0%
!! AAGCAACAAAAAGTACAAAA+GGG - chr8_1:24747794-24747813 None:intergenic 25.0%
!! AAAGCAACAAAAAGTACAAA+AGG - chr8_1:24747795-24747814 None:intergenic 25.0%
!! GAATATATTTGCCGTTTATT+TGG + chr8_1:24747855-24747874 Msa1186450:intron 25.0%
! GTATATTTATAGTTGTTCCT+AGG - chr8_1:24748293-24748312 None:intergenic 25.0%
! GAACAACTATAAATATACCT+AGG + chr8_1:24748295-24748314 Msa1186450:intron 25.0%
!! GAAGACTTTAGATTTACTTT+TGG + chr8_1:24748467-24748486 Msa1186450:intron 25.0%
!! TTCTGTTATTTAGAATTGTG+TGG + chr8_1:24748512-24748531 Msa1186450:intron 25.0%
! ACATTTCCCACAAATTAAAT+TGG - chr8_1:24748584-24748603 None:intergenic 25.0%
! GCATAATACAAATTAGTCAA+AGG - chr8_1:24748694-24748713 None:intergenic 25.0%
! ACAAAAGTTTATTGAGTGAA+TGG + chr8_1:24748744-24748763 Msa1186450:intron 25.0%
! TTTATTGAGTGAATGGATAA+TGG + chr8_1:24748751-24748770 Msa1186450:intron 25.0%
! AAATTAAGGTTTACTGTCAT+CGG - chr8_1:24748800-24748819 None:intergenic 25.0%
!! GGCTGAATTTTTCTTAAAAA+TGG + chr8_1:24748936-24748955 Msa1186450:intron 25.0%
! CTTAACACAATTTATGTCTA+AGG - chr8_1:24748976-24748995 None:intergenic 25.0%
! AGTCCTATTGTCTATAAATA+TGG + chr8_1:24749037-24749056 Msa1186450:intron 25.0%
! CTAAAAATTTATTGTTGCGT+TGG + chr8_1:24749499-24749518 Msa1186450:intron 25.0%
! CGATAAAAAAATATTCTGCA+TGG - chr8_1:24749632-24749651 None:intergenic 25.0%
TTTGTGTAATTGGATGAAGT+TGG + chr8_1:24747137-24747156 Msa1186450:intron 30.0%
TATGAAGACTAGATACTTGA+TGG - chr8_1:24747207-24747226 None:intergenic 30.0%
TAAGAATGAAGTAAAAAGGG+TGG - chr8_1:24747248-24747267 None:intergenic 30.0%
!!! TGTGATTTTTAGGACGTTTT+TGG + chr8_1:24747302-24747321 Msa1186450:intron 30.0%
AATAAAAACTGCTCACACAT+AGG - chr8_1:24747328-24747347 None:intergenic 30.0%
! ATGTGTGAGCAGTTTTTATT+TGG + chr8_1:24747328-24747347 Msa1186450:intron 30.0%
!!! TATTTGGTTTTGCTACTATG+AGG + chr8_1:24747344-24747363 Msa1186450:intron 30.0%
! GCATAGTTACTGTCAATTTT+TGG + chr8_1:24747381-24747400 Msa1186450:intron 30.0%
!! TAGTTACTGTCAATTTTTGG+TGG + chr8_1:24747384-24747403 Msa1186450:intron 30.0%
!!! AGTTACTGTCAATTTTTGGT+GGG + chr8_1:24747385-24747404 Msa1186450:intron 30.0%
GTTTACAATTACACAAGAAG+TGG + chr8_1:24747566-24747585 Msa1186450:intron 30.0%
!! TTTGGGTTTAGATTCTGTTT+TGG + chr8_1:24747682-24747701 Msa1186450:intron 30.0%
!!! CATAGTTGGGTTTTGTTTTT+TGG + chr8_1:24747742-24747761 Msa1186450:intron 30.0%
AAATATATTCATCGACGAGA+AGG - chr8_1:24747845-24747864 None:intergenic 30.0%
!! TTTTTGGAGGAATATTTCCT+AGG + chr8_1:24747957-24747976 Msa1186450:intron 30.0%
AAGAAACATTGAAGTTAGCT+AGG - chr8_1:24748394-24748413 None:intergenic 30.0%
!! CTTTTGGTGTGAAATTATTG+TGG + chr8_1:24748483-24748502 Msa1186450:intron 30.0%
!! TGGTCAACCAATTTAATTTG+TGG + chr8_1:24748574-24748593 Msa1186450:intron 30.0%
!! GGTCAACCAATTTAATTTGT+GGG + chr8_1:24748575-24748594 Msa1186450:intron 30.0%
! TTGTGCTATCTACATTGATT+CGG + chr8_1:24748636-24748655 Msa1186450:intron 30.0%
!! ATGGATAATGGTTTTTGACA+TGG + chr8_1:24748763-24748782 Msa1186450:intron 30.0%
!! TGGATAATGGTTTTTGACAT+GGG + chr8_1:24748764-24748783 Msa1186450:intron 30.0%
TCTAAGAGAGTAAGAATACT+AGG + chr8_1:24749124-24749143 Msa1186450:intron 30.0%
! AAAACATGTTAGCTTCTTGA+AGG - chr8_1:24749158-24749177 None:intergenic 30.0%
TATCACGCTAGAAAATAACA+TGG - chr8_1:24749237-24749256 None:intergenic 30.0%
TCTGACATATTACAACATTG+TGG + chr8_1:24749390-24749409 Msa1186450:intron 30.0%
!! TTTGGGTTTTCGATTGAAGA+TGG + chr8_1:24747016-24747035 Msa1186450:CDS 35.0%
! TTGGGTTTTCGATTGAAGAT+GGG + chr8_1:24747017-24747036 Msa1186450:CDS 35.0%
! AATCAACGTGTTTGAAGCTT+TGG + chr8_1:24747049-24747068 Msa1186450:CDS 35.0%
! ATCAACGTGTTTGAAGCTTT+GGG + chr8_1:24747050-24747069 Msa1186450:CDS 35.0%
TGTGTTTGTGAGTAATCGAT+TGG + chr8_1:24747102-24747121 Msa1186450:intron 35.0%
AGTAATCGATTGGAGGATTT+GGG + chr8_1:24747112-24747131 Msa1186450:intron 35.0%
GATTTGGGTGTTTGTGTAAT+TGG + chr8_1:24747127-24747146 Msa1186450:intron 35.0%
GGAGTTTACAACATCAATCA+TGG - chr8_1:24747465-24747484 None:intergenic 35.0%
ATGTCTAGCCAAGAAGATAA+GGG + chr8_1:24747503-24747522 Msa1186450:intron 35.0%
! ATGCTGTAGTGACAGATTTT+AGG + chr8_1:24747604-24747623 Msa1186450:intron 35.0%
!!! ACAGATTTTAGGTTCTGTTC+TGG + chr8_1:24747615-24747634 Msa1186450:intron 35.0%
! TGTTGCTATTGCGTCATATT+TGG + chr8_1:24747664-24747683 Msa1186450:intron 35.0%
! GTTGCTATTGCGTCATATTT+GGG + chr8_1:24747665-24747684 Msa1186450:intron 35.0%
! TGTTGTTGCTATTGCATAGT+TGG + chr8_1:24747728-24747747 Msa1186450:intron 35.0%
! GTTGTTGCTATTGCATAGTT+GGG + chr8_1:24747729-24747748 Msa1186450:intron 35.0%
!!! GGGTTTTGTTTTTTGGGTTT+AGG + chr8_1:24747749-24747768 Msa1186450:intron 35.0%
AAACTGTAATGTGTCAGTGA+TGG + chr8_1:24748182-24748201 Msa1186450:intron 35.0%
! TTTTTGCAGGAATGTTTCCT+AGG + chr8_1:24748219-24748238 Msa1186450:intron 35.0%
! GTTTCCTAGGAAATTTTCCT+AGG + chr8_1:24748232-24748251 Msa1186450:intron 35.0%
TCCTAGCTAACATATTTCCT+CGG + chr8_1:24748261-24748280 Msa1186450:intron 35.0%
TTAGCTAGGAAACATTTCCT+AGG - chr8_1:24748380-24748399 None:intergenic 35.0%
! TTTCCGTTTCATGTGAGTTT+TGG + chr8_1:24748554-24748573 Msa1186450:intron 35.0%
TGACCAAAACTCACATGAAA+CGG - chr8_1:24748560-24748579 None:intergenic 35.0%
AAACACCAGTAAGTACATGA+AGG - chr8_1:24748853-24748872 None:intergenic 35.0%
TTAGCTTCTTGAAGGAATGA+AGG - chr8_1:24749150-24749169 None:intergenic 35.0%
TCTAGCGTGATATGTAACTT+TGG + chr8_1:24749245-24749264 Msa1186450:intron 35.0%
TTGGATTATGAGTTTGAGAG+AGG + chr8_1:24749264-24749283 Msa1186450:intron 35.0%
ATTATGAGTTTGAGAGAGGT+TGG + chr8_1:24749268-24749287 Msa1186450:intron 35.0%
TACAAAGGACATTCGCAAAT+AGG - chr8_1:24749433-24749452 None:intergenic 35.0%
! TATTTTGTATACCTCCGATG+AGG + chr8_1:24749461-24749480 Msa1186450:intron 35.0%
!! CGATTGAAGATGGGTTTTAG+AGG + chr8_1:24747026-24747045 Msa1186450:CDS 40.0%
!! TTTGAAGCTTTGGGTGTTTG+TGG + chr8_1:24747059-24747078 Msa1186450:CDS 40.0%
GTTTGTGAGTAATCGATTGG+AGG + chr8_1:24747105-24747124 Msa1186450:intron 40.0%
GAGTAATCGATTGGAGGATT+TGG + chr8_1:24747111-24747130 Msa1186450:intron 40.0%
TTCACTTCAAGAACGAAGCT+TGG + chr8_1:24747162-24747181 Msa1186450:CDS 40.0%
GAAGACTAGATACTTGATGG+AGG - chr8_1:24747204-24747223 None:intergenic 40.0%
!! TTGGTGGGTCTGTGATTTTT+AGG + chr8_1:24747292-24747311 Msa1186450:intron 40.0%
CATGGCTGTCAATACAATGA+AGG - chr8_1:24747486-24747505 None:intergenic 40.0%
CTTATCTTCTTGGCTAGACA+TGG - chr8_1:24747504-24747523 None:intergenic 40.0%
CATGTCTAGCCAAGAAGATA+AGG + chr8_1:24747502-24747521 Msa1186450:intron 40.0%
CCTTCTTTCCCTTATCTTCT+TGG - chr8_1:24747514-24747533 None:intergenic 40.0%
CCAAGAAGATAAGGGAAAGA+AGG + chr8_1:24747511-24747530 Msa1186450:intron 40.0%
!!! ATTTTAGGTTCTGTTCTGGC+TGG + chr8_1:24747619-24747638 Msa1186450:intron 40.0%
!!! GGTTTAGATTCTGTTTTGGC+TGG + chr8_1:24747686-24747705 Msa1186450:intron 40.0%
!! TTAGGTGTGCCGCTTATATA+CGG + chr8_1:24747767-24747786 Msa1186450:intron 40.0%
CAAAAGGGACCGTATATAAG+CGG - chr8_1:24747779-24747798 None:intergenic 40.0%
CTCACCTAGGAAAATTTCCT+AGG - chr8_1:24748239-24748258 None:intergenic 40.0%
ACCGAGGAAATATGTTAGCT+AGG - chr8_1:24748265-24748284 None:intergenic 40.0%
TAGCTAACATATTTCCTCGG+TGG + chr8_1:24748264-24748283 Msa1186450:intron 40.0%
AGCTAACATATTTCCTCGGT+GGG + chr8_1:24748265-24748284 Msa1186450:intron 40.0%
!! ATACCTAGGAAGTGTTTCCT+CGG + chr8_1:24748309-24748328 Msa1186450:intron 40.0%
ACCATGCATACCTAGGAATT+TGG + chr8_1:24748346-24748365 Msa1186450:intron 40.0%
ACATGAAACGGAAACAGACA+AGG - chr8_1:24748548-24748567 None:intergenic 40.0%
! TGGTTTTTGACATGGGACTA+AGG + chr8_1:24748771-24748790 Msa1186450:intron 40.0%
! AAGAGCACGCTCTATTTCAT+GGG - chr8_1:24749310-24749329 None:intergenic 40.0%
AAAGGACATTCGCAAATAGG+AGG - chr8_1:24749430-24749449 None:intergenic 40.0%
CTTTGGGTGTTTGTGGATCT+GGG + chr8_1:24747066-24747085 Msa1186450:CDS 45.0%
TACACAAGAAGTGGAGCCAA+AGG + chr8_1:24747575-24747594 Msa1186450:intron 45.0%
ACTACAGCATCAGCAACCTT+TGG - chr8_1:24747594-24747613 None:intergenic 45.0%
!!! CTGGTTCTGATGCTGCATTT+TGG + chr8_1:24747638-24747657 Msa1186450:intron 45.0%
!!! TTGCTTTTTCCGCGAGTTGT+GGG + chr8_1:24747808-24747827 Msa1186450:intron 45.0%
CTGTAATGTGTCAGTGATGG+CGG + chr8_1:24748185-24748204 Msa1186450:intron 45.0%
!!! GGCTTGGTGTAGTTTTTTGC+AGG + chr8_1:24748206-24748225 Msa1186450:intron 45.0%
TTTCCGAGGAAACACTTCCT+AGG - chr8_1:24748315-24748334 None:intergenic 45.0%
TGTTTCCTCGGAAATGTCCT+AGG + chr8_1:24748321-24748340 Msa1186450:intron 45.0%
CTAGGTATGCATGGTTTCCT+AGG - chr8_1:24748341-24748360 None:intergenic 45.0%
CTAGGAAACCATGCATACCT+AGG + chr8_1:24748339-24748358 Msa1186450:intron 45.0%
GCCAAATTCCTAGGTATGCA+TGG - chr8_1:24748350-24748369 None:intergenic 45.0%
GGAAGAGAAGCCAAATTCCT+AGG - chr8_1:24748359-24748378 None:intergenic 45.0%
! GGAATTTGGCTTCTCTTCCT+AGG + chr8_1:24748360-24748379 Msa1186450:intron 45.0%
CATGAAGGACACGCATACTT+AGG - chr8_1:24748838-24748857 None:intergenic 45.0%
CGTGTCCTTCATGTACTTAC+TGG + chr8_1:24748845-24748864 Msa1186450:intron 45.0%
! CAAGAGCACGCTCTATTTCA+TGG - chr8_1:24749311-24749330 None:intergenic 45.0%
!!! TATTTAATTAACATAATTTT+AGG + chr8_1:24747911-24747930 Msa1186450:intron 5.0%
!!! ATTTAATTAACATAATTTTA+GGG + chr8_1:24747912-24747931 Msa1186450:intron 5.0%
!!! TTTAAAATTTGAAAATTTTT+TGG + chr8_1:24748050-24748069 Msa1186450:intron 5.0%
! GCTTTGGGTGTTTGTGGATC+TGG + chr8_1:24747065-24747084 Msa1186450:CDS 50.0%
ACTTGATGGAGGACACAGAG+AGG - chr8_1:24747193-24747212 None:intergenic 50.0%
!! CTGGCACTGATGCTGCATTT+AGG + chr8_1:24747705-24747724 Msa1186450:intron 50.0%
!!! GTTGCTTTTTCCGCGAGTTG+TGG + chr8_1:24747807-24747826 Msa1186450:intron 50.0%
TAAGACACACCCACAACTCG+CGG - chr8_1:24747820-24747839 None:intergenic 50.0%
GTTAGCTAGGACTCTCACCT+AGG - chr8_1:24748252-24748271 None:intergenic 50.0%
! TATTTCCTCGGTGGGTTCCT+AGG + chr8_1:24748273-24748292 Msa1186450:intron 50.0%
GGTTTCCTAGGACATTTCCG+AGG - chr8_1:24748329-24748348 None:intergenic 50.0%
TGTATACCTCCGATGAGGCT+CGG + chr8_1:24749466-24749485 Msa1186450:intron 50.0%
CAACAACAACCGAGCCTCAT+CGG - chr8_1:24749478-24749497 None:intergenic 50.0%
!! ATGTGTCAGTGATGGCGGCT+TGG + chr8_1:24748190-24748209 Msa1186450:intron 55.0%
TTGTTCCTAGGAACCCACCG+AGG - chr8_1:24748281-24748300 None:intergenic 55.0%
CAACAACCGAGCCTCATCGG+AGG - chr8_1:24749475-24749494 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 24747014 24749850 24747014 ID=Msa1186450;Name=Msa1186450
chr8_1 mRNA 24747014 24749850 24747014 ID=Msa1186450-mRNA-1;Parent=Msa1186450;Name=Msa1186450-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0|0|0|0.5|0.33|0.5|4|0|59
chr8_1 exon 24747014 24747087 24747014 ID=Msa1186450-mRNA-1:exon:8951;Parent=Msa1186450-mRNA-1
chr8_1 exon 24747156 24747228 24747156 ID=Msa1186450-mRNA-1:exon:8952;Parent=Msa1186450-mRNA-1
chr8_1 exon 24748906 24748911 24748906 ID=Msa1186450-mRNA-1:exon:8953;Parent=Msa1186450-mRNA-1
chr8_1 exon 24749824 24749850 24749824 ID=Msa1186450-mRNA-1:exon:8954;Parent=Msa1186450-mRNA-1
chr8_1 CDS 24747014 24747087 24747014 ID=Msa1186450-mRNA-1:cds;Parent=Msa1186450-mRNA-1
chr8_1 CDS 24747156 24747228 24747156 ID=Msa1186450-mRNA-1:cds;Parent=Msa1186450-mRNA-1
chr8_1 CDS 24748906 24748911 24748906 ID=Msa1186450-mRNA-1:cds;Parent=Msa1186450-mRNA-1
chr8_1 CDS 24749824 24749850 24749824 ID=Msa1186450-mRNA-1:cds;Parent=Msa1186450-mRNA-1
Gene Sequence

>Msa1186450

GGTTTGGGTTTTCGATTGAAGATGGGTTTTAGAGGAATCAACGTGTTTGAAGCTTTGGGTGTTTGTGGATCTGGTTGGATTTCACTTCAAGAACGAAGCTTGGATGCGCCTCTCTGTGTCCTCCATCAAGTATCTAGTCTTCATATTTCTTATATCTGTTTTCATGAAGCAATAGTTTGA

Protein sequence

>Msa1186450

GLGFRLKMGFRGINVFEALGVCGSGWISLQERSLDAPLCVLHQVSSLHISYICFHEAIV*