AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1187740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1187740 MtrunA17_Chr8g0354721 80.929 409 68 3 1 404 1093 1496 0.0 616
Msa1187740 MtrunA17_Chr8g0354671 62.698 378 128 6 32 398 7 382 2.87e-138 400
Msa1187740 MtrunA17_Chr8g0354711 50.120 415 150 9 1 398 1090 1464 3.04e-101 328
Msa1187740 MtrunA17_Chr8g0354701 48.309 414 156 10 1 398 1093 1464 2.83e-94 308
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 102 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACTCTTAATTGGAATTAT+TGG 0.146191 8_1:-27613554 None:intergenic
CAATGCAAGGATGGTCATTT+TGG 0.149849 8_1:-27613646 None:intergenic
CAATGCCTTGGAGCTGTTAA+TGG 0.207143 8_1:+27613982 Msa1187740:CDS
GCCATATAACCATATCTTCT+AGG 0.262623 8_1:+27613927 Msa1187740:CDS
GATAGAAAACCCTCCGTTAA+TGG 0.272984 8_1:+27613905 Msa1187740:CDS
TCCTCCAAAGGACTCTTAAT+TGG 0.283553 8_1:-27613563 None:intergenic
CTGAGGTGCAAGAGGCTATT+GGG 0.291403 8_1:+27614089 Msa1187740:CDS
CTCAATAAAAGAGATTTCTT+TGG 0.308348 8_1:+27614258 Msa1187740:CDS
GTTTGGACTTCATCAGCTAT+TGG 0.322325 8_1:+27614045 Msa1187740:CDS
AACACCGTATTCAGATCTTT+CGG 0.328820 8_1:-27613479 None:intergenic
CACCAGGAGATGTGGCTTCC+AGG 0.331072 8_1:-27613817 None:intergenic
CAACGGTGGATTGGAATTCT+TGG 0.334401 8_1:-27614141 None:intergenic
TCTTCTAGGGCTGCTTCTCT+TGG 0.335935 8_1:+27613941 Msa1187740:CDS
ACACCGTATTCAGATCTTTC+GGG 0.341505 8_1:-27613478 None:intergenic
TCTGATCCTGCTACACAGTT+TGG 0.350636 8_1:+27614028 Msa1187740:CDS
TTGTTCAAGGTCTCTTCCTT+TGG 0.354281 8_1:-27614353 None:intergenic
ATCATTCAAAGAAGCACAAT+TGG 0.371856 8_1:-27614425 None:intergenic
CTTGGAGCTGTTAATGGTTA+AGG 0.372486 8_1:+27613988 Msa1187740:CDS
GCTGAGGTGCAAGAGGCTAT+TGG 0.374843 8_1:+27614088 Msa1187740:CDS
TCACAAGAGGATACTGATTC+CGG 0.383766 8_1:-27613698 None:intergenic
TGTGTTGAATCTCAAGAATA+AGG 0.388931 8_1:+27614378 Msa1187740:CDS
CCCTAGAAGATATGGTTATA+TGG 0.395657 8_1:-27613928 None:intergenic
AATTCCAATTAAGAGTCCTT+TGG 0.398304 8_1:+27613559 Msa1187740:CDS
AGAAGCAGCCCTAGAAGATA+TGG 0.403368 8_1:-27613936 None:intergenic
CTGCCGAAGTAAAGCAGAAT+AGG 0.412534 8_1:+27614509 Msa1187740:CDS
TATTGGATGTATTGAATAGA+AGG 0.432373 8_1:+27614062 Msa1187740:CDS
AGTCTTCTTCCAACACTAAA+GGG 0.433805 8_1:+27613852 Msa1187740:CDS
AGAGATTTCTTTGGTCATCA+AGG 0.438878 8_1:+27614267 Msa1187740:CDS
GAATTATTGGTTGAGGGGCT+AGG 0.443809 8_1:-27613541 None:intergenic
TAATTGGAATTATTGGTTGA+GGG 0.445738 8_1:-27613547 None:intergenic
ATGTTTGTTCATATAATACA+TGG 0.452507 8_1:-27613097 None:intergenic
TTAATTGGAATTATTGGTTG+AGG 0.455696 8_1:-27613548 None:intergenic
GATTCAACACAAGTTGTTCA+AGG 0.459777 8_1:-27614366 None:intergenic
GAAAGTAGACGTAGACTCTT+CGG 0.460178 8_1:-27613729 None:intergenic
GAGTCTTCTTCCAACACTAA+AGG 0.462568 8_1:+27613851 Msa1187740:CDS
GGCCTGGAAGCCACATCTCC+TGG 0.462633 8_1:+27613815 Msa1187740:CDS
TTGGTGGTGGAGGATGGAAT+TGG 0.462698 8_1:-27613136 None:intergenic
AGTCCTTTGGAGGATGTCAT+AGG 0.473539 8_1:+27613572 Msa1187740:CDS
CAGAGTTATGTCAGAGGACC+AGG 0.475549 8_1:-27614542 None:intergenic
TATGGTTATATGGCCATTAA+CGG 0.478115 8_1:-27613918 None:intergenic
GTATTATATGAACAAACATC+TGG 0.481724 8_1:+27613101 Msa1187740:five_prime_UTR
TCCAATTAAGAGTCCTTTGG+AGG 0.488444 8_1:+27613562 Msa1187740:CDS
TCACAGACTCAACGGTGGAT+TGG 0.492138 8_1:-27614150 None:intergenic
TGAGCATGATGCTGTCCAAC+CGG 0.493265 8_1:+27613679 Msa1187740:CDS
GGTAGAAAACTCTCTATTAG+TGG 0.493725 8_1:+27613794 Msa1187740:CDS
AAACTCACATTTAGCATTGG+TGG 0.494777 8_1:-27613152 None:intergenic
ACGCAACCTCAACTTAATGA+TGG 0.496794 8_1:+27613509 Msa1187740:CDS
AAAACTCTCTATTAGTGGCC+TGG 0.497251 8_1:+27613799 Msa1187740:CDS
GACTCTTTAGATTTCGCACC+AGG 0.497865 8_1:-27613833 None:intergenic
CAAAGAGGCTATCAATGCCT+TGG 0.500456 8_1:+27613970 Msa1187740:CDS
CCATATAACCATATCTTCTA+GGG 0.505734 8_1:+27613928 Msa1187740:CDS
ACATTTAGCATTGGTGGTGG+AGG 0.515386 8_1:-27613146 None:intergenic
TCAACTTAATGATGGAGATA+AGG 0.517306 8_1:+27613517 Msa1187740:CDS
TTAGCATTGGTGGTGGAGGA+TGG 0.521575 8_1:-27613142 None:intergenic
TCAAAACTCACATTTAGCAT+TGG 0.522400 8_1:-27613155 None:intergenic
TGAAGTCCAAACTGTGTAGC+AGG 0.522447 8_1:-27614034 None:intergenic
TCATCAAGGCTGAAAATAAG+AGG 0.522722 8_1:+27614281 Msa1187740:CDS
GCATCTAGAGAGAATTCAGC+AGG 0.536073 8_1:+27614195 Msa1187740:CDS
AATTGGAATTATTGGTTGAG+GGG 0.544651 8_1:-27613546 None:intergenic
CTCACATTTAGCATTGGTGG+TGG 0.548723 8_1:-27613149 None:intergenic
GAGCCTATTCTGCTTTACTT+CGG 0.551403 8_1:-27614512 None:intergenic
TTTCTACCAACTCGGTCGAA+GGG 0.556624 8_1:-27613779 None:intergenic
ATGAACAAACATCTGGTTCG+AGG 0.559976 8_1:+27613108 Msa1187740:five_prime_UTR
CAGTTTGTTCACAGACTCAA+CGG 0.561861 8_1:-27614158 None:intergenic
AAGGACAGCTGAGGTGCAAG+AGG 0.564052 8_1:+27614081 Msa1187740:CDS
GCAGGAGACTGTCACAAGCA+AGG 0.571100 8_1:+27614213 Msa1187740:CDS
AGATTTCGCACCAGGAGATG+TGG 0.572718 8_1:-27613825 None:intergenic
TTATCTCCATCATTAAGTTG+AGG 0.576994 8_1:-27613515 None:intergenic
AGAGAGGTGGCTAGCTATTG+CGG 0.578111 8_1:+27613071 Msa1187740:five_prime_UTR
CACCGTATTCAGATCTTTCG+GGG 0.580009 8_1:-27613477 None:intergenic
GGCTGAAAATAAGAGGAAGA+AGG 0.580244 8_1:+27614288 Msa1187740:CDS
TATTGGGGAGTTCAAAAGAA+AGG 0.583080 8_1:+27614105 Msa1187740:CDS
AGAATAGGCTCAAGTCAACC+TGG 0.583585 8_1:+27614524 Msa1187740:CDS
TTAACCTTGACTCTTTGTTG+TGG 0.587592 8_1:-27613197 None:intergenic
TGAGTGTGACCCTTTAGTGT+TGG 0.590265 8_1:-27613861 None:intergenic
CAGGAGACTGTCACAAGCAA+GGG 0.590683 8_1:+27614214 Msa1187740:CDS
GTTATATGGCCATTAACGGA+GGG 0.590992 8_1:-27613914 None:intergenic
CTGTCACAAGCAAGGGTCGA+CGG 0.593525 8_1:+27614221 Msa1187740:CDS
TCTGATAGAGTCCTCACTGT+TGG 0.597838 8_1:-27613051 None:intergenic
TCATGACGTCTTTCAATGCA+AGG 0.603409 8_1:-27613659 None:intergenic
GATGACCCCTTCGACCGAGT+TGG 0.608239 8_1:+27613773 Msa1187740:CDS
TTCTACCAACTCGGTCGAAG+GGG 0.610811 8_1:-27613778 None:intergenic
TAAGAGGAAGAAGGAGCTAG+AGG 0.612316 8_1:+27614297 Msa1187740:CDS
GGTTATATGGCCATTAACGG+AGG 0.612642 8_1:-27613915 None:intergenic
TTCGGATTGAATTTCACAAG+AGG 0.620095 8_1:-27613711 None:intergenic
GACGTCTTTCAATGCAAGGA+TGG 0.621427 8_1:-27613655 None:intergenic
AAGAGGATACTGATTCCGGT+TGG 0.630275 8_1:-27613694 None:intergenic
TGAGGTGCAAGAGGCTATTG+GGG 0.632113 8_1:+27614090 Msa1187740:CDS
TCTACTTGCTGCGAAAGACG+AGG 0.632742 8_1:+27614480 Msa1187740:CDS
CTACTTGCTGCGAAAGACGA+GGG 0.638243 8_1:+27614481 Msa1187740:CDS
AAAGAACAACAAGATGCCAA+AGG 0.643115 8_1:+27614337 Msa1187740:CDS
TCTCCTATGACATCCTCCAA+AGG 0.657640 8_1:-27613575 None:intergenic
TGAGGACTCTATCAGAGAGG+TGG 0.661053 8_1:+27613058 Msa1187740:five_prime_UTR
TTCTCTTGGATCAATCAAAG+AGG 0.663230 8_1:+27613955 Msa1187740:CDS
CTTAACCATTAACAGCTCCA+AGG 0.664954 8_1:-27613987 None:intergenic
GAAACCACAACAAAGAGTCA+AGG 0.674078 8_1:+27613193 Msa1187740:CDS
ACCGTATTCAGATCTTTCGG+GGG 0.694725 8_1:-27613476 None:intergenic
TTTGTTCACAGACTCAACGG+TGG 0.706237 8_1:-27614155 None:intergenic
AATCTGCAGAGTTATGTCAG+AGG 0.716737 8_1:-27614548 None:intergenic
CAGTGAGGACTCTATCAGAG+AGG 0.721755 8_1:+27613055 Msa1187740:five_prime_UTR
ATTGAATAGAAGGACAGCTG+AGG 0.726649 8_1:+27614072 Msa1187740:CDS
GGATCATATTACCAACAGTG+AGG 0.773300 8_1:+27613040 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTTGTAAAAAATTG+AGG - chr8_1:27613251-27613270 None:intergenic 10.0%
!!! CAATTTTTTACAAAATAAAA+AGG + chr8_1:27613251-27613270 Msa1187740:intron 10.0%
!!! ATTTTTTACAAAATAAAAAG+GGG + chr8_1:27613253-27613272 Msa1187740:intron 10.0%
!!! TTTTATTTTGTAAAAAATTG+AGG - chr8_1:27613251-27613270 None:intergenic 10.0%
!!! CAATTTTTTACAAAATAAAA+AGG + chr8_1:27613251-27613270 Msa1187740:intron 10.0%
!!! ATTTTTTACAAAATAAAAAG+GGG + chr8_1:27613253-27613272 Msa1187740:intron 10.0%
!!! TTTTTTACAAAATAAAAAGG+GGG + chr8_1:27613254-27613273 Msa1187740:intron 15.0%
!!! TTTTTTACAAAATAAAAAGG+GGG + chr8_1:27613254-27613273 Msa1187740:intron 15.0%
!! ATGTTTGTTCATATAATACA+TGG - chr8_1:27613100-27613119 None:intergenic 20.0%
!!! AAAATCAAGCATAACATTTT+TGG + chr8_1:27614574-27614593 Msa1187740:CDS 20.0%
!! ATGTTTGTTCATATAATACA+TGG - chr8_1:27613100-27613119 None:intergenic 20.0%
!!! AAAATCAAGCATAACATTTT+TGG + chr8_1:27614574-27614593 Msa1187740:CDS 20.0%
! GTATTATATGAACAAACATC+TGG + chr8_1:27613101-27613120 Msa1187740:five_prime_UTR 25.0%
!!! GAGTTTTGATTCAAAATCAA+TGG + chr8_1:27613169-27613188 Msa1187740:CDS 25.0%
! AATTGAATATGAATCCAGAA+TGG - chr8_1:27613412-27613431 None:intergenic 25.0%
!! TAATTGGAATTATTGGTTGA+GGG - chr8_1:27613550-27613569 None:intergenic 25.0%
!! TTAATTGGAATTATTGGTTG+AGG - chr8_1:27613551-27613570 None:intergenic 25.0%
! ATAGGAGAAACTTCAAAAAA+TGG + chr8_1:27613590-27613609 Msa1187740:CDS 25.0%
! TATTGGATGTATTGAATAGA+AGG + chr8_1:27614062-27614081 Msa1187740:CDS 25.0%
! CTCAATAAAAGAGATTTCTT+TGG + chr8_1:27614258-27614277 Msa1187740:CDS 25.0%
! GTATTATATGAACAAACATC+TGG + chr8_1:27613101-27613120 Msa1187740:five_prime_UTR 25.0%
!!! GAGTTTTGATTCAAAATCAA+TGG + chr8_1:27613169-27613188 Msa1187740:CDS 25.0%
! AATTGAATATGAATCCAGAA+TGG - chr8_1:27613412-27613431 None:intergenic 25.0%
!! TAATTGGAATTATTGGTTGA+GGG - chr8_1:27613550-27613569 None:intergenic 25.0%
!! TTAATTGGAATTATTGGTTG+AGG - chr8_1:27613551-27613570 None:intergenic 25.0%
! ATAGGAGAAACTTCAAAAAA+TGG + chr8_1:27613590-27613609 Msa1187740:CDS 25.0%
! TATTGGATGTATTGAATAGA+AGG + chr8_1:27614062-27614081 Msa1187740:CDS 25.0%
! CTCAATAAAAGAGATTTCTT+TGG + chr8_1:27614258-27614277 Msa1187740:CDS 25.0%
TCAAAACTCACATTTAGCAT+TGG - chr8_1:27613158-27613177 None:intergenic 30.0%
! GTCAAGGTTAAATTTTCTTC+TGG + chr8_1:27613209-27613228 Msa1187740:intron 30.0%
TTACAAAATAAAAAGGGGGA+AGG + chr8_1:27613258-27613277 Msa1187740:intron 30.0%
TACAAAATAAAAAGGGGGAA+GGG + chr8_1:27613259-27613278 Msa1187740:intron 30.0%
! TTATCTCCATCATTAAGTTG+AGG - chr8_1:27613518-27613537 None:intergenic 30.0%
! TCAACTTAATGATGGAGATA+AGG + chr8_1:27613517-27613536 Msa1187740:CDS 30.0%
! AATTGGAATTATTGGTTGAG+GGG - chr8_1:27613549-27613568 None:intergenic 30.0%
GGACTCTTAATTGGAATTAT+TGG - chr8_1:27613557-27613576 None:intergenic 30.0%
AATTCCAATTAAGAGTCCTT+TGG + chr8_1:27613559-27613578 Msa1187740:CDS 30.0%
AACTTCAAAAAATGGTAGAG+AGG + chr8_1:27613598-27613617 Msa1187740:CDS 30.0%
TATGGTTATATGGCCATTAA+CGG - chr8_1:27613921-27613940 None:intergenic 30.0%
CCATATAACCATATCTTCTA+GGG + chr8_1:27613928-27613947 Msa1187740:CDS 30.0%
TGTGTTGAATCTCAAGAATA+AGG + chr8_1:27614378-27614397 Msa1187740:CDS 30.0%
! ATCATTCAAAGAAGCACAAT+TGG - chr8_1:27614428-27614447 None:intergenic 30.0%
TCAAAACTCACATTTAGCAT+TGG - chr8_1:27613158-27613177 None:intergenic 30.0%
! GTCAAGGTTAAATTTTCTTC+TGG + chr8_1:27613209-27613228 Msa1187740:intron 30.0%
TTACAAAATAAAAAGGGGGA+AGG + chr8_1:27613258-27613277 Msa1187740:intron 30.0%
TACAAAATAAAAAGGGGGAA+GGG + chr8_1:27613259-27613278 Msa1187740:intron 30.0%
! TTATCTCCATCATTAAGTTG+AGG - chr8_1:27613518-27613537 None:intergenic 30.0%
! TCAACTTAATGATGGAGATA+AGG + chr8_1:27613517-27613536 Msa1187740:CDS 30.0%
! AATTGGAATTATTGGTTGAG+GGG - chr8_1:27613549-27613568 None:intergenic 30.0%
GGACTCTTAATTGGAATTAT+TGG - chr8_1:27613557-27613576 None:intergenic 30.0%
AATTCCAATTAAGAGTCCTT+TGG + chr8_1:27613559-27613578 Msa1187740:CDS 30.0%
AACTTCAAAAAATGGTAGAG+AGG + chr8_1:27613598-27613617 Msa1187740:CDS 30.0%
TATGGTTATATGGCCATTAA+CGG - chr8_1:27613921-27613940 None:intergenic 30.0%
CCATATAACCATATCTTCTA+GGG + chr8_1:27613928-27613947 Msa1187740:CDS 30.0%
TGTGTTGAATCTCAAGAATA+AGG + chr8_1:27614378-27614397 Msa1187740:CDS 30.0%
! ATCATTCAAAGAAGCACAAT+TGG - chr8_1:27614428-27614447 None:intergenic 30.0%
! AAACTCACATTTAGCATTGG+TGG - chr8_1:27613155-27613174 None:intergenic 35.0%
TTAACCTTGACTCTTTGTTG+TGG - chr8_1:27613200-27613219 None:intergenic 35.0%
ACAAAATAAAAAGGGGGAAG+GGG + chr8_1:27613260-27613279 Msa1187740:intron 35.0%
CTTAGTGACGGTTTGTAAAA+TGG - chr8_1:27613324-27613343 None:intergenic 35.0%
! CATTTTACAAACCGTCACTA+AGG + chr8_1:27613322-27613341 Msa1187740:intron 35.0%
GTATCAAATCTCCTTAGTGA+CGG - chr8_1:27613336-27613355 None:intergenic 35.0%
!! TACAACTGAGTTGATACTTG+TGG + chr8_1:27613369-27613388 Msa1187740:intron 35.0%
GATACTTGTGGACAAATTCA+AGG + chr8_1:27613381-27613400 Msa1187740:intron 35.0%
CTCTGTTACAATCTGGAAAA+AGG - chr8_1:27613453-27613472 None:intergenic 35.0%
AACACCGTATTCAGATCTTT+CGG - chr8_1:27613482-27613501 None:intergenic 35.0%
TTCGGATTGAATTTCACAAG+AGG - chr8_1:27613714-27613733 None:intergenic 35.0%
! TAGAGAGTTTTCTACCAACT+CGG - chr8_1:27613790-27613809 None:intergenic 35.0%
GGTAGAAAACTCTCTATTAG+TGG + chr8_1:27613794-27613813 Msa1187740:CDS 35.0%
AGTCTTCTTCCAACACTAAA+GGG + chr8_1:27613852-27613871 Msa1187740:CDS 35.0%
GCCATATAACCATATCTTCT+AGG + chr8_1:27613927-27613946 Msa1187740:CDS 35.0%
CCCTAGAAGATATGGTTATA+TGG - chr8_1:27613931-27613950 None:intergenic 35.0%
TTCTCTTGGATCAATCAAAG+AGG + chr8_1:27613955-27613974 Msa1187740:CDS 35.0%
TATTGGGGAGTTCAAAAGAA+AGG + chr8_1:27614105-27614124 Msa1187740:CDS 35.0%
AGAGATTTCTTTGGTCATCA+AGG + chr8_1:27614267-27614286 Msa1187740:CDS 35.0%
TCATCAAGGCTGAAAATAAG+AGG + chr8_1:27614281-27614300 Msa1187740:CDS 35.0%
AAAGAACAACAAGATGCCAA+AGG + chr8_1:27614337-27614356 Msa1187740:CDS 35.0%
GATTCAACACAAGTTGTTCA+AGG - chr8_1:27614369-27614388 None:intergenic 35.0%
! AAACTCACATTTAGCATTGG+TGG - chr8_1:27613155-27613174 None:intergenic 35.0%
TTAACCTTGACTCTTTGTTG+TGG - chr8_1:27613200-27613219 None:intergenic 35.0%
ACAAAATAAAAAGGGGGAAG+GGG + chr8_1:27613260-27613279 Msa1187740:intron 35.0%
CTTAGTGACGGTTTGTAAAA+TGG - chr8_1:27613324-27613343 None:intergenic 35.0%
! CATTTTACAAACCGTCACTA+AGG + chr8_1:27613322-27613341 Msa1187740:intron 35.0%
GTATCAAATCTCCTTAGTGA+CGG - chr8_1:27613336-27613355 None:intergenic 35.0%
!! TACAACTGAGTTGATACTTG+TGG + chr8_1:27613369-27613388 Msa1187740:intron 35.0%
GATACTTGTGGACAAATTCA+AGG + chr8_1:27613381-27613400 Msa1187740:intron 35.0%
CTCTGTTACAATCTGGAAAA+AGG - chr8_1:27613453-27613472 None:intergenic 35.0%
AACACCGTATTCAGATCTTT+CGG - chr8_1:27613482-27613501 None:intergenic 35.0%
TTCGGATTGAATTTCACAAG+AGG - chr8_1:27613714-27613733 None:intergenic 35.0%
! TAGAGAGTTTTCTACCAACT+CGG - chr8_1:27613790-27613809 None:intergenic 35.0%
GGTAGAAAACTCTCTATTAG+TGG + chr8_1:27613794-27613813 Msa1187740:CDS 35.0%
AGTCTTCTTCCAACACTAAA+GGG + chr8_1:27613852-27613871 Msa1187740:CDS 35.0%
GCCATATAACCATATCTTCT+AGG + chr8_1:27613927-27613946 Msa1187740:CDS 35.0%
CCCTAGAAGATATGGTTATA+TGG - chr8_1:27613931-27613950 None:intergenic 35.0%
TTCTCTTGGATCAATCAAAG+AGG + chr8_1:27613955-27613974 Msa1187740:CDS 35.0%
TATTGGGGAGTTCAAAAGAA+AGG + chr8_1:27614105-27614124 Msa1187740:CDS 35.0%
AGAGATTTCTTTGGTCATCA+AGG + chr8_1:27614267-27614286 Msa1187740:CDS 35.0%
TCATCAAGGCTGAAAATAAG+AGG + chr8_1:27614281-27614300 Msa1187740:CDS 35.0%
AAAGAACAACAAGATGCCAA+AGG + chr8_1:27614337-27614356 Msa1187740:CDS 35.0%
GATTCAACACAAGTTGTTCA+AGG - chr8_1:27614369-27614388 None:intergenic 35.0%
! ATGAACAAACATCTGGTTCG+AGG + chr8_1:27613108-27613127 Msa1187740:five_prime_UTR 40.0%
GAAACCACAACAAAGAGTCA+AGG + chr8_1:27613193-27613212 Msa1187740:CDS 40.0%
AATTCAAGGTTGCTCCATTC+TGG + chr8_1:27613395-27613414 Msa1187740:intron 40.0%
ACACCGTATTCAGATCTTTC+GGG - chr8_1:27613481-27613500 None:intergenic 40.0%
ACGCAACCTCAACTTAATGA+TGG + chr8_1:27613509-27613528 Msa1187740:CDS 40.0%
TCCAATTAAGAGTCCTTTGG+AGG + chr8_1:27613562-27613581 Msa1187740:CDS 40.0%
TCCTCCAAAGGACTCTTAAT+TGG - chr8_1:27613566-27613585 None:intergenic 40.0%
! CAATGCAAGGATGGTCATTT+TGG - chr8_1:27613649-27613668 None:intergenic 40.0%
TCATGACGTCTTTCAATGCA+AGG - chr8_1:27613662-27613681 None:intergenic 40.0%
!! TCACAAGAGGATACTGATTC+CGG - chr8_1:27613701-27613720 None:intergenic 40.0%
GAAAGTAGACGTAGACTCTT+CGG - chr8_1:27613732-27613751 None:intergenic 40.0%
AAAACTCTCTATTAGTGGCC+TGG + chr8_1:27613799-27613818 Msa1187740:CDS 40.0%
GAGTCTTCTTCCAACACTAA+AGG + chr8_1:27613851-27613870 Msa1187740:CDS 40.0%
GATAGAAAACCCTCCGTTAA+TGG + chr8_1:27613905-27613924 Msa1187740:CDS 40.0%
GTTATATGGCCATTAACGGA+GGG - chr8_1:27613917-27613936 None:intergenic 40.0%
CTTAACCATTAACAGCTCCA+AGG - chr8_1:27613990-27614009 None:intergenic 40.0%
CTTGGAGCTGTTAATGGTTA+AGG + chr8_1:27613988-27614007 Msa1187740:CDS 40.0%
GTTTGGACTTCATCAGCTAT+TGG + chr8_1:27614045-27614064 Msa1187740:CDS 40.0%
ATTGAATAGAAGGACAGCTG+AGG + chr8_1:27614072-27614091 Msa1187740:CDS 40.0%
! CAGTTTGTTCACAGACTCAA+CGG - chr8_1:27614161-27614180 None:intergenic 40.0%
GGCTGAAAATAAGAGGAAGA+AGG + chr8_1:27614288-27614307 Msa1187740:CDS 40.0%
TTGTTCAAGGTCTCTTCCTT+TGG - chr8_1:27614356-27614375 None:intergenic 40.0%
GAGCCTATTCTGCTTTACTT+CGG - chr8_1:27614515-27614534 None:intergenic 40.0%
AATCTGCAGAGTTATGTCAG+AGG - chr8_1:27614551-27614570 None:intergenic 40.0%
! ATGAACAAACATCTGGTTCG+AGG + chr8_1:27613108-27613127 Msa1187740:five_prime_UTR 40.0%
GAAACCACAACAAAGAGTCA+AGG + chr8_1:27613193-27613212 Msa1187740:CDS 40.0%
AATTCAAGGTTGCTCCATTC+TGG + chr8_1:27613395-27613414 Msa1187740:intron 40.0%
ACACCGTATTCAGATCTTTC+GGG - chr8_1:27613481-27613500 None:intergenic 40.0%
ACGCAACCTCAACTTAATGA+TGG + chr8_1:27613509-27613528 Msa1187740:CDS 40.0%
TCCAATTAAGAGTCCTTTGG+AGG + chr8_1:27613562-27613581 Msa1187740:CDS 40.0%
TCCTCCAAAGGACTCTTAAT+TGG - chr8_1:27613566-27613585 None:intergenic 40.0%
! CAATGCAAGGATGGTCATTT+TGG - chr8_1:27613649-27613668 None:intergenic 40.0%
TCATGACGTCTTTCAATGCA+AGG - chr8_1:27613662-27613681 None:intergenic 40.0%
!! TCACAAGAGGATACTGATTC+CGG - chr8_1:27613701-27613720 None:intergenic 40.0%
GAAAGTAGACGTAGACTCTT+CGG - chr8_1:27613732-27613751 None:intergenic 40.0%
AAAACTCTCTATTAGTGGCC+TGG + chr8_1:27613799-27613818 Msa1187740:CDS 40.0%
GAGTCTTCTTCCAACACTAA+AGG + chr8_1:27613851-27613870 Msa1187740:CDS 40.0%
GATAGAAAACCCTCCGTTAA+TGG + chr8_1:27613905-27613924 Msa1187740:CDS 40.0%
GTTATATGGCCATTAACGGA+GGG - chr8_1:27613917-27613936 None:intergenic 40.0%
CTTAACCATTAACAGCTCCA+AGG - chr8_1:27613990-27614009 None:intergenic 40.0%
CTTGGAGCTGTTAATGGTTA+AGG + chr8_1:27613988-27614007 Msa1187740:CDS 40.0%
GTTTGGACTTCATCAGCTAT+TGG + chr8_1:27614045-27614064 Msa1187740:CDS 40.0%
ATTGAATAGAAGGACAGCTG+AGG + chr8_1:27614072-27614091 Msa1187740:CDS 40.0%
! CAGTTTGTTCACAGACTCAA+CGG - chr8_1:27614161-27614180 None:intergenic 40.0%
GGCTGAAAATAAGAGGAAGA+AGG + chr8_1:27614288-27614307 Msa1187740:CDS 40.0%
TTGTTCAAGGTCTCTTCCTT+TGG - chr8_1:27614356-27614375 None:intergenic 40.0%
GAGCCTATTCTGCTTTACTT+CGG - chr8_1:27614515-27614534 None:intergenic 40.0%
AATCTGCAGAGTTATGTCAG+AGG - chr8_1:27614551-27614570 None:intergenic 40.0%
TCTGATAGAGTCCTCACTGT+TGG - chr8_1:27613054-27613073 None:intergenic 45.0%
!! ACATTTAGCATTGGTGGTGG+AGG - chr8_1:27613149-27613168 None:intergenic 45.0%
!! CTCACATTTAGCATTGGTGG+TGG - chr8_1:27613152-27613171 None:intergenic 45.0%
GGGGTTTCTCTGTTACAATC+TGG - chr8_1:27613460-27613479 None:intergenic 45.0%
ACCCCCGAAAGATCTGAATA+CGG + chr8_1:27613475-27613494 Msa1187740:CDS 45.0%
ACCGTATTCAGATCTTTCGG+GGG - chr8_1:27613479-27613498 None:intergenic 45.0%
CACCGTATTCAGATCTTTCG+GGG - chr8_1:27613480-27613499 None:intergenic 45.0%
! GAATTATTGGTTGAGGGGCT+AGG - chr8_1:27613544-27613563 None:intergenic 45.0%
! AGTCCTTTGGAGGATGTCAT+AGG + chr8_1:27613572-27613591 Msa1187740:CDS 45.0%
TCTCCTATGACATCCTCCAA+AGG - chr8_1:27613578-27613597 None:intergenic 45.0%
GACGTCTTTCAATGCAAGGA+TGG - chr8_1:27613658-27613677 None:intergenic 45.0%
!! AAGAGGATACTGATTCCGGT+TGG - chr8_1:27613697-27613716 None:intergenic 45.0%
TTTCTACCAACTCGGTCGAA+GGG - chr8_1:27613782-27613801 None:intergenic 45.0%
! TTTTCTACCAACTCGGTCGA+AGG - chr8_1:27613783-27613802 None:intergenic 45.0%
GACTCTTTAGATTTCGCACC+AGG - chr8_1:27613836-27613855 None:intergenic 45.0%
TGAGTGTGACCCTTTAGTGT+TGG - chr8_1:27613864-27613883 None:intergenic 45.0%
GGTTATATGGCCATTAACGG+AGG - chr8_1:27613918-27613937 None:intergenic 45.0%
AGAAGCAGCCCTAGAAGATA+TGG - chr8_1:27613939-27613958 None:intergenic 45.0%
CAAAGAGGCTATCAATGCCT+TGG + chr8_1:27613970-27613989 Msa1187740:CDS 45.0%
CAATGCCTTGGAGCTGTTAA+TGG + chr8_1:27613982-27614001 Msa1187740:CDS 45.0%
TCTGATCCTGCTACACAGTT+TGG + chr8_1:27614028-27614047 Msa1187740:CDS 45.0%
TGAAGTCCAAACTGTGTAGC+AGG - chr8_1:27614037-27614056 None:intergenic 45.0%
!! CAACGGTGGATTGGAATTCT+TGG - chr8_1:27614144-27614163 None:intergenic 45.0%
TTTGTTCACAGACTCAACGG+TGG - chr8_1:27614158-27614177 None:intergenic 45.0%
GCATCTAGAGAGAATTCAGC+AGG + chr8_1:27614195-27614214 Msa1187740:CDS 45.0%
TAAGAGGAAGAAGGAGCTAG+AGG + chr8_1:27614297-27614316 Msa1187740:CDS 45.0%
CTGCCGAAGTAAAGCAGAAT+AGG + chr8_1:27614509-27614528 Msa1187740:CDS 45.0%
AGAATAGGCTCAAGTCAACC+TGG + chr8_1:27614524-27614543 Msa1187740:CDS 45.0%
TCTGATAGAGTCCTCACTGT+TGG - chr8_1:27613054-27613073 None:intergenic 45.0%
!! ACATTTAGCATTGGTGGTGG+AGG - chr8_1:27613149-27613168 None:intergenic 45.0%
!! CTCACATTTAGCATTGGTGG+TGG - chr8_1:27613152-27613171 None:intergenic 45.0%
GGGGTTTCTCTGTTACAATC+TGG - chr8_1:27613460-27613479 None:intergenic 45.0%
ACCCCCGAAAGATCTGAATA+CGG + chr8_1:27613475-27613494 Msa1187740:CDS 45.0%
ACCGTATTCAGATCTTTCGG+GGG - chr8_1:27613479-27613498 None:intergenic 45.0%
CACCGTATTCAGATCTTTCG+GGG - chr8_1:27613480-27613499 None:intergenic 45.0%
! GAATTATTGGTTGAGGGGCT+AGG - chr8_1:27613544-27613563 None:intergenic 45.0%
! AGTCCTTTGGAGGATGTCAT+AGG + chr8_1:27613572-27613591 Msa1187740:CDS 45.0%
TCTCCTATGACATCCTCCAA+AGG - chr8_1:27613578-27613597 None:intergenic 45.0%
GACGTCTTTCAATGCAAGGA+TGG - chr8_1:27613658-27613677 None:intergenic 45.0%
!! AAGAGGATACTGATTCCGGT+TGG - chr8_1:27613697-27613716 None:intergenic 45.0%
TTTCTACCAACTCGGTCGAA+GGG - chr8_1:27613782-27613801 None:intergenic 45.0%
! TTTTCTACCAACTCGGTCGA+AGG - chr8_1:27613783-27613802 None:intergenic 45.0%
GACTCTTTAGATTTCGCACC+AGG - chr8_1:27613836-27613855 None:intergenic 45.0%
TGAGTGTGACCCTTTAGTGT+TGG - chr8_1:27613864-27613883 None:intergenic 45.0%
GGTTATATGGCCATTAACGG+AGG - chr8_1:27613918-27613937 None:intergenic 45.0%
AGAAGCAGCCCTAGAAGATA+TGG - chr8_1:27613939-27613958 None:intergenic 45.0%
CAAAGAGGCTATCAATGCCT+TGG + chr8_1:27613970-27613989 Msa1187740:CDS 45.0%
CAATGCCTTGGAGCTGTTAA+TGG + chr8_1:27613982-27614001 Msa1187740:CDS 45.0%
TCTGATCCTGCTACACAGTT+TGG + chr8_1:27614028-27614047 Msa1187740:CDS 45.0%
TGAAGTCCAAACTGTGTAGC+AGG - chr8_1:27614037-27614056 None:intergenic 45.0%
!! CAACGGTGGATTGGAATTCT+TGG - chr8_1:27614144-27614163 None:intergenic 45.0%
TTTGTTCACAGACTCAACGG+TGG - chr8_1:27614158-27614177 None:intergenic 45.0%
GCATCTAGAGAGAATTCAGC+AGG + chr8_1:27614195-27614214 Msa1187740:CDS 45.0%
TAAGAGGAAGAAGGAGCTAG+AGG + chr8_1:27614297-27614316 Msa1187740:CDS 45.0%
CTGCCGAAGTAAAGCAGAAT+AGG + chr8_1:27614509-27614528 Msa1187740:CDS 45.0%
AGAATAGGCTCAAGTCAACC+TGG + chr8_1:27614524-27614543 Msa1187740:CDS 45.0%
!!! AATTTTTTACAAAATAAAAA+GGG + chr8_1:27613252-27613271 Msa1187740:intron 5.0%
!!! AATTTTTTACAAAATAAAAA+GGG + chr8_1:27613252-27613271 Msa1187740:intron 5.0%
CAGTGAGGACTCTATCAGAG+AGG + chr8_1:27613055-27613074 Msa1187740:five_prime_UTR 50.0%
TGAGGACTCTATCAGAGAGG+TGG + chr8_1:27613058-27613077 Msa1187740:five_prime_UTR 50.0%
! AGAGAGGTGGCTAGCTATTG+CGG + chr8_1:27613071-27613090 Msa1187740:five_prime_UTR 50.0%
!! TTGGTGGTGGAGGATGGAAT+TGG - chr8_1:27613139-27613158 None:intergenic 50.0%
!! TTAGCATTGGTGGTGGAGGA+TGG - chr8_1:27613145-27613164 None:intergenic 50.0%
TGAGCATGATGCTGTCCAAC+CGG + chr8_1:27613679-27613698 Msa1187740:CDS 50.0%
TTCTACCAACTCGGTCGAAG+GGG - chr8_1:27613781-27613800 None:intergenic 50.0%
AGATTTCGCACCAGGAGATG+TGG - chr8_1:27613828-27613847 None:intergenic 50.0%
!! TCTTCTAGGGCTGCTTCTCT+TGG + chr8_1:27613941-27613960 Msa1187740:CDS 50.0%
CTGAGGTGCAAGAGGCTATT+GGG + chr8_1:27614089-27614108 Msa1187740:CDS 50.0%
TGAGGTGCAAGAGGCTATTG+GGG + chr8_1:27614090-27614109 Msa1187740:CDS 50.0%
TCACAGACTCAACGGTGGAT+TGG - chr8_1:27614153-27614172 None:intergenic 50.0%
CAGGAGACTGTCACAAGCAA+GGG + chr8_1:27614214-27614233 Msa1187740:CDS 50.0%
! TCTACTTGCTGCGAAAGACG+AGG + chr8_1:27614480-27614499 Msa1187740:CDS 50.0%
! CTACTTGCTGCGAAAGACGA+GGG + chr8_1:27614481-27614500 Msa1187740:CDS 50.0%
CAGAGTTATGTCAGAGGACC+AGG - chr8_1:27614545-27614564 None:intergenic 50.0%
CAGTGAGGACTCTATCAGAG+AGG + chr8_1:27613055-27613074 Msa1187740:five_prime_UTR 50.0%
TGAGGACTCTATCAGAGAGG+TGG + chr8_1:27613058-27613077 Msa1187740:five_prime_UTR 50.0%
! AGAGAGGTGGCTAGCTATTG+CGG + chr8_1:27613071-27613090 Msa1187740:five_prime_UTR 50.0%
!! TTGGTGGTGGAGGATGGAAT+TGG - chr8_1:27613139-27613158 None:intergenic 50.0%
!! TTAGCATTGGTGGTGGAGGA+TGG - chr8_1:27613145-27613164 None:intergenic 50.0%
TGAGCATGATGCTGTCCAAC+CGG + chr8_1:27613679-27613698 Msa1187740:CDS 50.0%
TTCTACCAACTCGGTCGAAG+GGG - chr8_1:27613781-27613800 None:intergenic 50.0%
AGATTTCGCACCAGGAGATG+TGG - chr8_1:27613828-27613847 None:intergenic 50.0%
!! TCTTCTAGGGCTGCTTCTCT+TGG + chr8_1:27613941-27613960 Msa1187740:CDS 50.0%
CTGAGGTGCAAGAGGCTATT+GGG + chr8_1:27614089-27614108 Msa1187740:CDS 50.0%
TGAGGTGCAAGAGGCTATTG+GGG + chr8_1:27614090-27614109 Msa1187740:CDS 50.0%
TCACAGACTCAACGGTGGAT+TGG - chr8_1:27614153-27614172 None:intergenic 50.0%
CAGGAGACTGTCACAAGCAA+GGG + chr8_1:27614214-27614233 Msa1187740:CDS 50.0%
! TCTACTTGCTGCGAAAGACG+AGG + chr8_1:27614480-27614499 Msa1187740:CDS 50.0%
! CTACTTGCTGCGAAAGACGA+GGG + chr8_1:27614481-27614500 Msa1187740:CDS 50.0%
CAGAGTTATGTCAGAGGACC+AGG - chr8_1:27614545-27614564 None:intergenic 50.0%
AAGGACAGCTGAGGTGCAAG+AGG + chr8_1:27614081-27614100 Msa1187740:CDS 55.0%
GCTGAGGTGCAAGAGGCTAT+TGG + chr8_1:27614088-27614107 Msa1187740:CDS 55.0%
GCAGGAGACTGTCACAAGCA+AGG + chr8_1:27614213-27614232 Msa1187740:CDS 55.0%
CTGTCACAAGCAAGGGTCGA+CGG + chr8_1:27614221-27614240 Msa1187740:CDS 55.0%
AAGGACAGCTGAGGTGCAAG+AGG + chr8_1:27614081-27614100 Msa1187740:CDS 55.0%
GCTGAGGTGCAAGAGGCTAT+TGG + chr8_1:27614088-27614107 Msa1187740:CDS 55.0%
GCAGGAGACTGTCACAAGCA+AGG + chr8_1:27614213-27614232 Msa1187740:CDS 55.0%
CTGTCACAAGCAAGGGTCGA+CGG + chr8_1:27614221-27614240 Msa1187740:CDS 55.0%
GATGACCCCTTCGACCGAGT+TGG + chr8_1:27613773-27613792 Msa1187740:CDS 60.0%
CACCAGGAGATGTGGCTTCC+AGG - chr8_1:27613820-27613839 None:intergenic 60.0%
GATGACCCCTTCGACCGAGT+TGG + chr8_1:27613773-27613792 Msa1187740:CDS 60.0%
CACCAGGAGATGTGGCTTCC+AGG - chr8_1:27613820-27613839 None:intergenic 60.0%
GGCCTGGAAGCCACATCTCC+TGG + chr8_1:27613815-27613834 Msa1187740:CDS 65.0%
GGCCTGGAAGCCACATCTCC+TGG + chr8_1:27613815-27613834 Msa1187740:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 27613044 27614618 27613044 ID=Msa1187740;Name=Msa1187740
chr8_1 mRNA 27613044 27614618 27613044 ID=Msa1187740-mRNA-1;Parent=Msa1187740;Name=Msa1187740-mRNA-1;_AED=0.43;_eAED=0.44;_QI=114|0|0.5|1|1|1|2|0|404
chr8_1 exon 27613044 27613214 27613044 ID=Msa1187740-mRNA-1:exon:9706;Parent=Msa1187740-mRNA-1
chr8_1 exon 27613461 27614618 27613461 ID=Msa1187740-mRNA-1:exon:9707;Parent=Msa1187740-mRNA-1
chr8_1 five_prime_UTR 27613044 27613157 27613044 ID=Msa1187740-mRNA-1:five_prime_utr;Parent=Msa1187740-mRNA-1
chr8_1 CDS 27613158 27613214 27613158 ID=Msa1187740-mRNA-1:cds;Parent=Msa1187740-mRNA-1
chr8_1 CDS 27613461 27614618 27613461 ID=Msa1187740-mRNA-1:cds;Parent=Msa1187740-mRNA-1
Gene Sequence

>Msa1187740

ATGCTAAATGTGAGTTTTGATTCAAAATCAATGGCGAAACCACAACAAAGAGTCAAGATTGTAACAGAGAAACCCCCGAAAGATCTGAATACGGTGTTATCTTTGACGCAACCTCAACTTAATGATGGAGATAAGGTCCTAGCCCCTCAACCAATAATTCCAATTAAGAGTCCTTTGGAGGATGTCATAGGAGAAACTTCAAAAAATGGTAGAGAGGATGAAGAAGCTTATGCTATTACAATCCAAAATGACCATCCTTGCATTGAAAGACGTCATGAGCATGATGCTGTCCAACCGGAATCAGTATCCTCTTGTGAAATTCAATCCGAAGAGTCTACGTCTACTTTCAACACTAAAGATTCTGACTCAGATGACCCCTTCGACCGAGTTGGTAGAAAACTCTCTATTAGTGGCCTGGAAGCCACATCTCCTGGTGCGAAATCTAAAGAGTCTTCTTCCAACACTAAAGGGTCACACTCAGATTATGTCATCGACTTAGTTGATAGAAAACCCTCCGTTAATGGCCATATAACCATATCTTCTAGGGCTGCTTCTCTTGGATCAATCAAAGAGGCTATCAATGCCTTGGAGCTGTTAATGGTTAAGGACTTATCTGAAGTCTCTTCTGATCCTGCTACACAGTTTGGACTTCATCAGCTATTGGATGTATTGAATAGAAGGACAGCTGAGGTGCAAGAGGCTATTGGGGAGTTCAAAAGAAAGGCAGTCGCATCATGCCAAGAATTCCAATCCACCGTTGAGTCTGTGAACAAACTGAAGAGTTATGAGAAGCATCTAGAGAGAATTCAGCAGGAGACTGTCACAAGCAAGGGTCGACGGAATGATCTCAAAAACTCAATAAAAGAGATTTCTTTGGTCATCAAGGCTGAAAATAAGAGGAAGAAGGAGCTAGAGGCAGAAATTGATAGTTTGAAAGAACAACAAGATGCCAAAGGAAGAGACCTTGAACAACTTGTGTTGAATCTCAAGAATAAGGAAGCAACACTCTCAACTTATTCAACCAATTGTGCTTCTTTGAATGATAAAGCTCAAAAGCTATTAGAAGAAGCTGATGTTCTACTTGCTGCGAAAGACGAGGGCGAAGCTGCCGAAGTAAAGCAGAATAGGCTCAAGTCAACCTGGTCCTCTGACATAACTCTGCAGATTAGTAAAATCAAGCATAACATTTTTGGTTCAGTTCACAATGAATGTTGA
Protein sequence

>Msa1187740

MLNVSFDSKSMAKPQQRVKIVTEKPPKDLNTVLSLTQPQLNDGDKVLAPQPIIPIKSPLEDVIGETSKNGREDEEAYAITIQNDHPCIERRHEHDAVQPESVSSCEIQSEESTSTFNTKDSDSDDPFDRVGRKLSISGLEATSPGAKSKESSSNTKGSHSDYVIDLVDRKPSVNGHITISSRAASLGSIKEAINALELLMVKDLSEVSSDPATQFGLHQLLDVLNRRTAEVQEAIGEFKRKAVASCQEFQSTVESVNKLKSYEKHLERIQQETVTSKGRRNDLKNSIKEISLVIKAENKRKKELEAEIDSLKEQQDAKGRDLEQLVLNLKNKEATLSTYSTNCASLNDKAQKLLEEADVLLAAKDEGEAAEVKQNRLKSTWSSDITLQISKIKHNIFGSVHNEC*